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BC-GLA-NHS
This product was discontinued on 01/05/2015
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Properties and Usage
References
Description
BC-GLA-NHS is an amine-reactive building block for the one-step synthesis of CLIP-tag™ substrates from amine-containing precursors
including proteins, peptides or oligonucleotides. This building block allows you to make custom CLIP-tag substrates for labeling CLIP-tag
fusion proteins.
The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is
a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In
the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a
stable thioether link. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class
of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and
complementary labeling of two proteins simultaneously in the same cells.
There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the
fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIPtag plasmids. The labeling of the fusion proteins with the CLIP-tag substrate is described in this document.
Figure 1. Structure of BC-GLA-NHS (MW 441.4 g/mol)
Properties and Usage
Storage Temperature
-20°C
Notes
1. BC-GLA-NHS is supplied in lyophilized form and packaged under argon. The dry CLIP-tag building block substrate should be stored at 20°C.
2. BC-GLA-NHS is soluble in anhydrous DMF or DMSO. Choose a solvent volume that will be compatible with the final use. Vortex and
centrifuge briefly to remove any non-dissolved solids.
3. NHS esters readily hydrolyze in moist environments. Experiments should be designed to maximize the usage of BC-GLA-NHS.
Storage of unused BC-GLA-NHS solubilized in DMF or DMSO should be avoided. If unavoidable, we recommend storing at -20°C for the
minimum time period.
References
1. Gauthier, A. et al. (2008). Chemistry & Biology. 5, 128-136.
FAQs
FAQs
1. Cellular Imaging and Analysis FAQs
Protocols
Datacards
Protocols
1. Reaction Conditions for Chemical Coupling (S9237)
2. Labeling CLIP-tag Fusion Proteins with BC-substrates (S9237)
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
S9237Datasheet-Lot0031210
Selection Charts
Interactive Tools
Troubleshooting Guides
Selection Charts
Building Blocks
Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
Troubleshooting Guides
Labeling with SNAP-tag® Technology Troubleshooting Guide
Interactive Tools
NEBioCalculator
Quality Control
Datacards
Safety Data Sheet
Quality Control
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product.
Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting
Documents section of this page. Further information regarding NEB product quality can be found here.
Physical Purity (HPLC):
The purity of the product is determined by HPLC analysis.
Safety Data Sheet
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
BC-GLA-NHS
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
S9237Datasheet-Lot0031210
Legal and Disclaimers
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc
(NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain
additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes
in humans or animals.
Licenses
Cellular Imaging and Analysis (i.e., SNAP and CLIP products)
Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH
AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England
Biolabs, Inc., 240 County Road, Ipswich, MA 01938. For detailed information see our Terms of Use .
The products and/or their use may be covered by one or more of the following patents and patent applications:
7,939,284 (Methods for Using O6-Alkylguanine-DNA-Alkyltransferases)
7,888,090 (Mutants of O6-Alkylguanine-DNA-Alkyltransferases)
8,163,479 Specific Substrates for O6-Alkylguanine-DNA-Alkyltransferases)
8,178,314 (Pyrimidines Reacting With O6-Alkylguanine-DNA-Alkyltransferases)
PCT/EP2007/057597 (Labeling of Fusion Proteins with Synthetic Probes)
EP07117800 (Drug Delivery)
EP07117802 (Drug Delivery)
EP07120288 (GTPase-Transient Protein Protein Interactions)
These patents and patent applications are owned by Covalys, or owned by the Ecole Polytechnique Fédérale de Lausanne (EPFL)
and exclusively licensed to Covalys and NEB.