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Cell Line Specification Sheet
Walker-PY
Rat carcinoma cells expressing PhiYFP
SPECIFICATIONS:
Cell Line: Walker 256 cells expressing PhiYFP
Cells/vial: approximately 5 x 105
Subculturing: 1:3 to 1:6
Medium Renewal: 2 to 3 times per week
Growth Medium: RPMI 1640 + 10 % fetal calf serum (FCS), 2 mM L-Alanyl-L-Glutamine, 1
% Non Essential Amino Acids (NEA)
Freeze Medium: complete growth medium + 10 % FCS, 10 % DMSO
mycoplasma-free
Shipping: frozen
Storage Recommendation: Liquid nitrogen
Biosafety Level: 1
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Tel.: +49-6221-71415-16 Fax: +49-6221-71415-29 E-mail: [email protected] Internet www.biocat.com
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Information:
Organism: Rattus norvegicus (rat)
Source:
Organ: breast
Disease: carcinoma
Gender:
female
Growth properties: suspension
Tumorigenic:
yes
Morphology:
spherical
Conditions:
37 °C, 5 % CO2
Plasmid:
pPhiYFP
(Evrogen, Moscow, Russia)
Walker 256 cells expressing PhiYFP
Walker 256 (rat breast carcinoma) cells were stably transfected with a
plasmid coding for PhiYFP to establish this bright yellow fluorescent cell
line.
Walker is a rapidly growing transplantable rat tumor. It resembles a
carcinoma in young transplants and a sarcoma in older transplants.
(Stedman, 25th ed)
The Walker tumor originally appeared spontaneously in the mammary
gland of a pregnant albino rat. This suspension cell line constitutes a wellknown metastasizing carcinoma model and can be used for screening of
putative anti-tumor agents.
Sold by BioCat GmbH Technologiepark Heidelberg
Im Neuenheimer Feld 584 - 69120 Heidelberg - Germany
Tel.: +49-6221-71415-16 Fax: +49-6221-71415-29 E-mail: [email protected] Internet www.biocat.com
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References
Pavlaki M, Giannopoulou E, Niarakis A, Ravazoula P, Aletras AJ. Walker 256 cancer cells secrete
tissue inhibitor of metalloproteinase-free metalloproteinase-9.Mol Cell Biochem. 2009 Aug;328(12):189-99. Epub 2009 Mar 29. PMID: 19330523.
Tandon VK et al. Design, synthesis and evaluation of novel 1,4-naphtoquinone derivatives as
antifungal and anticancer agents. Bioorg Med Chem Lett. 14:1079-83, 2004 PMID: 14980639.
Shetlar MR et al. Serum polysaccharide levels in rats bearing the Walker 256 tumor. Cancer Res 10:
445-7, 1950 PMID: 15427084.
Arvelo F, Yabrudi A, Delgado ME, González-Cadavid N. Establishment and characterization of cell
lines from the Walker carcinoma 256 able to grow in suspension culture and deficient in thymidine
kinase. In Vitro. 1984 Jul;20(7):549-65. PMID: 6469274
Hull RN et al. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor
cells on glass. Science 117: 223-5, 1953 PMID: 13038481.
Fearon KC et al. Reduction of the growth rate of the Walker 256 tumor in rats by rhodamine 6G
together with hypoglycemia. Cancer Res 47: 3684-7, 1987 PMID: 3594433.
Zaletok S et al. Role of polyamines in the function of nuclear transcription factor NF-kappaB in
breast cancer cells. Exp Oncol. 26:221-5, 2004 PMID: 15494691.
Blouin S et al. Relations between radiograph texture analysis and microcomputed tomography in
two rat models of bone metastases. Cells Tissues Organs 182: 182-92, 2006 PMID: 16914920.
FOR NON-HUMAN INVESTIGATIONAL RESEARCH ONLY.
TO HANDLE UNDER AT LEAST BIOSAFETY LEVEL 1 CONTAINMENT.
Information derived from ECACC, cell-lines-service and Stedman, 25th ed.
Sold by BioCat GmbH Technologiepark Heidelberg
Im Neuenheimer Feld 584 - 69120 Heidelberg - Germany
Tel.: +49-6221-71415-16 Fax: +49-6221-71415-29 E-mail: [email protected] Internet www.biocat.com