DATA
A SHEET
Rat monoclo
m
onal an
nti mou
use Gr-11 (Ly-66G)
(A
Azide-free & Low en
ndotoxin)
ORD
DERING IN
NFORMATION
Catalog
C
Num
mber:
Size:
Storage:
Im
mmunogen::
Ig
g Type:
Clone
C
Endotoxin
E
Level
L
Applications
A
s:
gA
AP-0059
1.0
00 mg
< -20°
C
Mo
ouse sponta
aneous T lym
mphoma lin
ne TK1
Ra
at IgG2
AP
P-MAB0866
6
IgG*
< 0.002EU/μg
0
FC
C, IHC (Frozen/paraffin
n), and Dep
pletion
Desccription: Gr--1 is a 21-25
5 kD protein
n also known
n as Ly-6G/L
Ly-6C. Thiss myeloid diifferentiationn antigen is a
glycoosylphosphaatidylinositol (GPI)-link
ked protein expressed on granuloocytes and macrophagges. In bonee
marrrow, the expression levels of Gr-1 diirectly correelate with graanulocyte diifferentiationn and maturaation; Gr-1 is
also transiently expressed on
o bone maarrow cells in the monnocyte lineage. Gr-1+ ccells have reecently been
n
impllicated in thee developmeent of antitum
mor responsees.
Prepparation: Th
his antibody was produceed from a hy
ybridoma (m
mouse myelom
ma fused wiith spleen cells from a raat
imm
munized with Mouse spontaneous T lymphoma
a line TK1.
Form
mulation: The IgG fracttion of cultu
ure superna
atant was puurified by P
Protein A/G affinity chroomatography
y
and llyophilized from
f
a 0.2 µm
µ filtered so
olution in ph
hosphate-bufffered salinee (PBS, Azid
de Free).
Recoonstitution: Reconstitutee the antibod
dy with steriile PBS and the reconstiituted antiboody can be aaliquoted and
d
storeed frozen at < -20 for at
a least for six
s months without
w
deteectable loss of activity. Avoid repeeated freezethaw
w cycles. Lyo
ophilized sam
mples are stable for 2 yeears from daate of receiptt when storedd at -70°C.
* En
ndotoxin Levvel:
Extreemely low leevel of LPS (<
( 0.002EU//μg IgG)
Appllication(s):
1.
2.
3.
FC
F and IP
IH
HC (Frozen
n and Parafffin)
In
n vivo depleetion of PMN
N and Myelloid suppre ssor cells
* The antibody is produced by in
n vitro culturee and For Reseearch Use Onlly