PAT H O L O G Y
Dako Antibodies Dako Antibodies to
Mismatch Repair Proteins
The mismatch
repair antibodies
Introducing antibodies to MSH2, MSH6 and PMS2
Dako mismatch repair portfolio includes
MSH2, MSH6, PMS2 and MLH1 antibodies
and are used for the diagnosis of colorectal
cancer and Lynch syndrome.
The mismatch repair
antibodies
For the diagnosis of colorectal cancer
and Lynch syndrome
The mismatch repair proteins MSH2, MSH6, PMS2 and MLH1 are nuclear
proteins involved in the DNA mismatch repair (MMR) pathway. The MMR
pathway is utilized by normal proliferating cells to repair mutations that
may occur during DNA replication.
Deficiency or loss of MMR function in the gastrointestinal tract results in an
increased mutation rate and contributes to the development of sporadic
colorectal carcinoma as well as hereditary non-polyposis colorectal
carcinoma (HNPCC) also called Lynch syndrome. The four antibodies
are useful for identifying mismatch repair deficiencies in tumors of the
gastrointestinal tract including HNPCC, and associated extra-colonic
cancers using immunohistochemistry (1,2).
Monoclonal Mouse Anti-Human MutS
Protein Homolog 2 (MSH2), Clone FE11
Colon adenocarcinoma (FFPE) with loss of
MSH2 protein. Stained with FLEX Anti-MSH2,
Code IR085, on Autostainer Link 48.
2
Monoclonal Rabbit Anti-Human MutS
Protein Homolog 6 (MSH6), Clone EP49
Colon adenocarcinoma (FFPE) with loss of
MSH6 protein. Stained with FLEX Anti-MSH6,
Code IR086, on Autostainer Link 48.
Loss of MMR activity
may result from:
„„A
hereditary mutation in a
gene(s) coding for a MMR
protein(s)
„„A spontaneous loss of MMR
activity, in some cases caused
by methylation-induced gene
silencing
Monoclonal Rabbit Anti-Human Postmeiotic Segregation Increased 2 (PMS2), Clone EP51
Colon adenocarcinoma (FFPE) with loss of
PMS2 protein. Stained with FLEX Anti-PMS2,
Code IR087, on Autostainer Link 48.
Dako introduces three high quality rabbit and mouse
monoclonal antibodies to mismatch repair proteins
„„Monoclonal
MLH1
Mouse Anti-Human MutS Protein Homolog 2,
Clone FE11 NEW
„„Monoclonal Rabbit Anti-Human MutS Protein Homolog 6,
Clone EP49 NEW
„„Monoclonal Rabbit Anti-Human Postmeiotic Segregation Increased 2,
Clone EP51 NEW
„„Monoclonal Mouse Anti-Human MutL Protein Homolog 1,
Clone ES05
PMS2
MSH6
MSH2
A
G
MMR and DNA string showing how the
MMR proteins work in pairs.
Loss of one or more mismatch repair proteins
is often associated with the loss of a coupled
protein. MLH1 and PMS2 loss are often paired.
MSH2 and MSH6 loss are often paired.
All four in-vitro diagnostic antibodies are available as concentrates and
FLEX Ready-To-Use (RTU) primary antibodies for use on formalin-fixed,
paraffin-embedded tissue sections.
FLEX RTU antibodies are optimized for the Autostainer Link platform.
Dako EnVisionTM FLEX validated protocol information is retrievable through
bar-coded labeling. Together, the Dako FLEX RTU antibodies
and EnVisionTM FLEX visualization system provide:
„„Consistently
high diagnostic certainty
validated protocol
„„Efficient workflow
„„Optimized,
Monoclonal Mouse Anti-Human MutL
Protein Homolog 1 (MLH1), Clone ES05
Colon adenocarcinoma (FFPE) with loss of
MLH1 protein. Stained with FLEX Anti-MLH1,
Code IR079, on Autostainer Link 48.
3
Ordering information
The mismatch repair antibodies are available both as FLEX RTU antibodies and as concentrates.
Antibody
Description
Platform
Order Number
Monoclonal Mouse Anti-Human MutS
Protein Homolog 2 , Clone FE11
FLEX RTU 60 tests, 12 mL
Autostainer Link 48
IR08561
Concentrate 1 mL
-
M363901
Concentrate 0.2 mL
-
M363929
FLEX RTU 60 tests, 12 mL
Autostainer Link 48
IR08661
Concentrate 1 mL
-
M364601
Concentrate 0.2 mL
-
M364629
FLEX RTU 60 tests, 12 mL
Autostainer Link 48
IR08761
Concentrate 1 mL
-
M364701
Concentrate 0.2 mL
-
M364729
FLEX RTU 60 tests, 12 mL
Autostainer Link 48
IR07961
FLEX RTU 30 tets, 12 mL
Dako Autostainer
instruments
IS07930
Concentrate 1 mL
-
M364001
Concentrate 0.2 mL
-
M364029
Monoclonal Rabbit Anti-Human MutS
Protein Homolog 6, Clone EP49
Monoclonal Rabbit Anti-Human Postmeiotic
Segregation Increased 2, Clone EP51
Monoclonal Mouse Anti-Human MutL
Protein Homolog 1, clone ES05
References:
1. Peltomäki P. Role of DNA mismatch repair defects in the pathogenesis of human cancer. J Clin Oncol 2003;21:1174-79.
2. Lynch H. Smyrk, T. Hereditary nonpolyposis colorectal cancer (Lynch syndrome). An updated review. Cancer 1996; 78:1149-67.
Australia
+61 3 9357 0892
Canada
+1 905 335 3256
France
+33 1 30 50 00 50
Japan
+81 3 5802 7211
Spain
+34 93 499 05 06
Austria
+43 1 408 43 34 0
China
+86 21 6327 1122
Germany
+49 40 69 69 470
The Netherlands
+31 20 42 11 100
Sweden
+46 8 556 20 600
www.dako.com
Belgium
+32 (0) 16 38 72 20
Denmark
+45 44 85 97 56
Ireland
+353 1 479 0568
Norway
+47 23 14 05 40
Switzerland
+41 41 760 11 66
Represented in more
than 80 countries
Brazil
+55 11 50708300
Finland
+358 9 348 73 950
Italy
+39 02 58 078 1
Poland
+48 58 661 1879
United Kingdom
+44 (0)1 353 66 99 11
United States of America
+1 805 566 6655
29044 21AUG12
Corporate Headquarters
Denmark
+45 44 85 95 00