Arthropod Management Tests 2014, Vol. 39 doi: 10.4182/amt.2014.G5 (G5) ROSE: Rosa ‘Belinda’s Dream’ and ‘Caldwell Pink’ FRANKLINIELLA TRITICI (FITCH) CONTROL WITH SEVERAL BIOINSECTICIDES, 2012 O. Milo Lewis Department of Entomology Texas A&M University 412 Minnie Belle Heep TAMU 2475 College Station, TX 77843-2475 Phone: 979-845-6305 Fax: 979.845.6305 Email: [email protected] Peter C. Krauter Email: [email protected] Kevin M. Heinz Email: [email protected] Eastern Flower Thrips (EFT): Frankliniella tritici (Fitch) The objective of the study was to determine the efficacy of bioinsecticide formulations to control EFT infestations of potted commercial roses. Tests were conducted in the Antique Rose Emporium’s outdoor potted rose nursery in Independence, TX. There were a total of 12 treatments, defined by trade name and application rate (Tables 1 and 2). There were thirty-six roses per plot, arranged six roses by six roses placed side by side, each in two gallon liners. Treatments were replicated six times with four replications in cv. ‘Belinda’s Dream’ and two replications in cv. ‘Caldwell Pink’. Data from the two cultivars were pooled. To sample for EFT, three fully expanded rose flowers from each plot were arbitrarily removed, inserted into plastic containers, and placed into cooler for transport to the laboratory. Since plots were side-by-side, rose flowers that were near the edge of the plot were not selected. Within two days of sample collection, the petals from the three flowers from each plot were pulled apart and inserted into another plastic container. The plastic container was vigorously shaken four separate times, after each vigorous shaking, EFT were extracted (via aspiration) into glass alcohol vials from the container walls until no EFT were found. Immature (larvae and pupae) and adult EFT were counted separately under a stereomicroscope. A pretreatment census was completed 8 days before making the initial treatment applications, and treatments designations were assigned using a stratified random process resulting in no statistical differences in mean thrips densities among treatments. Insecticides were applied using a two-gallon hand pump sprayer with a flat jet nozzle. Foliage and flowers were sprayed until runoff was achieved. When sampling and spray applications were made on the same day, sampling preceded spray applications. The minimum, maximum and average daily temperature sampled at thirty minute intervals during the trial was 5.40, 31.52, and 16.98°C (n=48), respectively, with no unusual weather events. Application and sampling dates are detailed in Tables 1 and 2. Since raw data were non-normally distributed (Shapiro-Wilk Test, P < 0.05) and the square root(x) nor the log(x) data transformation resulted in a normalized distribution of data, the non-parametric Kruskal-Wallis test was used to determine significance. The Kruskal-Wallis test indicated no significant differences in mean number of immature and adult EFT per plot when comparing treatments within sample dates and the overall mean across sample dates, excluding precounts. All funding receives for the project was provided by the IR-4 Program. 1 Arthropod Management Tests 2014, Vol. 39 doi: 10.4182/amt.2014.G5 Table 1. Mean number of immature EFT per plot Name Rate A16901B A16901B Azaguard MBI203 1 MBI203 1 MBI206 1 MBI206 1 Proud 3 Avid Conserve 2 Water Untreated 6.7 oz/100 gal solution 13.4 oz/100 gal solution 16 fl oz/100 gal solution 2 lb/100 gal solution 4 lb/100 gal solution 1 gal/100 gal solution 2 gal/100 gal solution 1 gal/100 gal solution 16 fl oz/100 gal solution 8 fl oz/100 gal solution ----- Application date 26 Oct, 8 Nov, 26 Nov 26 Oct, 8 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov 26 Oct, 8 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov --- 18 Oct 3 4 2 Nov 8 Nov 16 Nov 28 Nov 5 Dec Mean 1.67 6.50 2.33 3.00 10.67 3.00 5.10 2.33 3.67 3.33 2.17 3.67 1.83 2.93 0.50 4.00 1.83 5.00 12.33 3.80 5.45 0.67 3.67 3.17 4.50 8.67 3.67 4.73 0.17 6.17 3.67 3.67 8.83 2.00 4.87 0.17 5.50 5.00 4.67 7.00 5.00 5.45 1.00 5.67 10.00 7.83 4.83 2.83 6.23 1.67 4.67 2.83 2.50 8.50 1.17 3.93 0.83 3.67 3.50 3.33 5.50 3.83 3.97 0.33 1.67 2.83 2.83 7.17 3.50 3.60 0.50 10.50 5.17 3.67 3.17 2.33 4.97 0.83 3.33 4.33 12.17 3.80 5.34 2.83 1 2 3 4 Hyper-Active spray adjuvant used at 2 pt/100 gal solution. Grower’s tap water. Precount. Mean across weeks, excluding 18 Oct. No significant differences were found within columns (Kruskal-Wallis Test, P < 0.05). Table 2. Mean number of adult EFT per plot Name Rate A16901B 6.7 oz/100 gal solution A16901B 13.4 oz/100 gal solution Azaguard 16 fl oz/100 gal solution 1 2 lb/100 MBI203 gal solution 1 4 lb/100 MBI203 gal solution 1 1 gal/100 MBI206 gal solution 1 2 gal/100 MBI206 gal solution Proud 3 1 gal/100 gal solution Avid 16 fl oz/100 gal solution Conserve 8 fl oz/100 gal solution 2 Water Untreated 1 Application date 26 Oct, 8 Nov, 26 Nov 26 Oct, 8 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov, 8 Nov, 16 Nov, 26 Nov 26 Oct, 2 Nov 18 Oct 3 4 2 Nov 8 Nov 16 Nov 28 Nov 5 Dec Mean 19.17 31.50 34.50 47.67 78.83 51.17 46.60 18.17 40.50 37.67 44.83 61.50 44.33 45.03 16.50 23.50 17.17 56.50 64.17 44.20 41.00 17.50 29.17 32.67 66.33 87.67 71.50 55.73 16.83 34.83 43.33 63.67 88.50 55.17 55.33 19.17 31.17 25.50 64.67 54.50 50.00 43.55 16.17 28.67 58.17 59.83 49.67 49.50 48.20 16.50 42.00 35.33 78.33 62.17 46.33 51.13 15.67 27.17 30.83 31.83 58.33 56.67 39.87 26 Oct, 8 Nov, 15.83 40.17 21.50 26 Nov 26 Oct, 2 Nov, 8 Nov, 16.17 45.17 28.67 16 Nov, 26 Nov --15.67 34.50 35.83 39.17 63.50 85.17 48.47 37.67 74.00 87.33 53.93 38.33 79.83 65.20 47.72 2 3 4 Hyper-Active spray adjuvant used at 2 pt/100 gal solution. Grower's tap water. Precount. Mean across weeks, excluding 18 Oct. No significant differences were found within columns (Kruskal-Wallis Test, P < 0.05). 2
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