,Ill/sro-tocu
unl
2015
( 3rd Semester )
BIOTECHNOLOGY
Paper No. :
Bf,-III
( Mlcroblologr I
FlaLlt
.
Ma*s
Time
:
z 55
2Yz lr'ottrs
( Pent : B-DESCRIPTIVE
( Marks : 35
)
)
tle margin ildiute full
.for tle qtestions
Tte figares in
'
Answer
marks
all questions
1. (c) Write an account on germ theory.
7
(b) Describe the contribution of
John
Tyndall on spontaneous generation and
Alocarder Fleming in penicillin
development.
3Yz+3lz=7
2. (a) W}:at is binomial nomenclature?
Write
the concept of binomial nomenclature of
l+6=7
microbes.
G16/63a
( TDn Ouer )
t2t
a1
fb,l
Illustrate the distinguishing features of
archeal membrane system with suitable
diagrams.
7
t. (a) Define potato
dextrose agar
(PDA)
medium. Discuss the dillerence between .
I+3+3=7
solid and liquid medium.
Or
chemical method of
sterilization? Discuss dry heat and
l+3+3=7
Iiltration sterilization,
Ib,l What is
4. [a/
Write short notes on the following
:
?ta+3%=7
(,
Streak plate method
(a/ Flagela staining
(b) Vlhat is
grams staining? Explain the
procedure and principle involved in
grams staining,
1+3+3=7
5. (a) What is endospore? Write an ac@unt on
sporulation process in bacteria. 1+6=7
Or
b)
Wnt.c short notes on the following :
3%+3Y2=7
(i,
(ii)
Concept of exponential growth
Dry weight ceU count
***
G16-roo/63a
III/BIo-TECH (iii)
III/rro-tEcH
(iiit
2015
( 3rd Semester
)
BIOTECHNOLOGY
Paper No. : BT-III
(
Microbtology
I
( PART : A-oBJECTIVE
( Marks: 20
The
)
\
figures in t?w margin indicate futl marks for the questions
Answer all questions
SECTToN-A
(
Marks:5)
Tick (/) the correct answer in the brackets provided
1. Which scientist
first disproved
:
1x5=5
spontaneous
generation by showing that maggots only appear on
decaying meat that has been exposed to flies?
Redi (
(b/ Joseph Lister
(
(c) Louis Pasteur (
/d/ Robert Hooke (
/a,) Francesco
163
)
)
)
)
t2l
2.
The following statements are correct with respect to
nomenclature of microbes, excepf
/a/
Names of all taxa are printed in italics and
( )
should be underlined if hand written.
(b) 'Sp.'is used for any of the species
whereas 'Spp.'
(
species.
is used for a particular
)
(c) The genus name is always capitalized,
species name
capitalized. (
is never
the
)
(d) Binary nomenclature (invented by Linnaeus), is
consisting of a generic name and a species
epithet.
(
)
3. The medium which is used to isolate and
differentiate members of the enterobacteriaceae is
called
/a/
Eosin Methylene Blue
1bl MacConkey agar
(
/d/ Mannitol salt agar
(c)
Blood
agar
(EMB)
( )
(
)
)
(
)
4. The stain that is used for capsule staining is
(
dye
( )
@/ Congo red
(c) malachite green (
ldJ safrosin ( )
/a/
nigrosine
rrr/Bro-TEcH (iii)/53
)
)
(3)
Exponential phase of growth curve of bacteria is of
limited duration because of
density ( )
accumulation of toxic products (
/c/ exhaustion of nutrients ( )
(d) All of the above ( )
[a/
fb/
raise in cell
rrr/Bro-rEcH (iii)/63
)
(41
SEcTIoN-B
(Marks:
Write short notes on the
15
)
following
1, Louis Pasteur's contribution
generation
3x5=15
in
spontaneous
3
rrrlBro-TEcH (in)/63
t,
,!1
(5)
2.
Mycelium
III/BIO_TECH (iii)/63
3
l6l
3. Principle of autoclave
rr/Bro-TECH (iii)/63
J
t7t
4. (a) Rrre culture
1
(bJ Advantages of acid-fast staining
1
(c)
1
Negative staining
rrrlBro-TECH (iii)/63
,!1
(81
5. Bacterial turbidity
3
***
Gi6-100/63
rrr/Bro-TECH {iii)