Mendel and His Peas Lecture Learning Objectives: You will be able to: LO1 – To describe Mendel’s work and the hypotheses it generated LO2– To explain how Mendel’s results lead to the principles of particulate inheritance and independent inheritance. 2/29/2012 2 LO1 – To describe Mendel’s work and the hypotheses it generated 2/29/2012 3 Observations Driving Mendel’s Work Plant and Animal Breeders noticed that offspring (hybrids) of 2 different individuals often displayed an intermediate like a mix of parental traits. Hypothesis: Blending inheritance predicts…. 2/29/2012 4 Controlling Fertilization of the Flowers 2/29/2012 5 Prediction of Blending Hypothesis Predict that F1 generation have slightly wrinkled seeds 2/29/2012 6 Mendel’s Monohybrid Cross Results Parental Generation= pure breeding lines Offspring – F1 generation– phenotype of male parent Phenotype of female parent not present Does this support the Blending Hypothesis? Why or Why not? 2/29/2012 7 Where did the Wrinkled Trait Go ? Critical Experiment –female phenotype in F1 is not lost, so “blending “did not occur 2/29/2012 8 Your Turn Work the monohybrid cross problems in the handout. 2/29/2012 9 Think About It…… Thanks to digital photography and programs like Photoshop, photography studios can claim to predict what a couple’s child would look like based upon merging images of the putative parents. Explain in genetic terms why this is nonsense. 2/29/2012 10 Mendel’s Unfortunate Naming Conventions What is the nature of the dominant allele? What is the nature of the recessive allele? Dominate and recessive represent alternative phenotypes. Dominant does not imply presence, Recessive does not imply absence 2/29/2012 11 Does Gender Influence Trait Inheritance? The Reciprocal Cross 2/29/2012 12 Results of Mendel’s 8 Years of Experiments What do you notice about the ratio of dominant to recessive phenotype in each cross? Is the distribution of offspring dependent on the trait studied? 2/29/2012 13 The Dihybrid Cross and Independent Assortment Do the parental alleles remain together when gametes are formed? 2/29/2012 14 Your Turn: Predict the Gametes for Dependent Assortment Female Parent: rryy ( green seeds, wrinkled) Male parent: RRYY (yellow seeds, smooth Gametes produced by Female Parent: ry Gametes produced by Male Parent: RY RrYy Phenotypes of F2 Generation: ¼ RYRY, ½ Ryry, ¼ ryry Phenotypes of F1 Generation: 2/29/2012 15 Mendel’s result of Dihybrid Cross Which of Mendel’s hypotheses is consistent with these results? 2/29/2012 16 Your Turn Work the dihybrid cross problems in your handout 2/29/2012 17 But What If You Know The Phenotype But Not The Genotype: The Test Cross A test cross reveals an unknown genotype Why do we care? Contribution of homozygous parent known. Allows determination of 2nd parental phenotype. 2/29/2012 18 Lecture Summary How did Mendel’s work discredit the hypothesis of Blended Inheritance? What evidence did Mendel use to support the claim that traits are particulate? What evidence supports the principle of independent segregation? 2/29/2012 19
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