“ a world of learning”
Stentor
We supply:
Living materials
Catalogue Code: L 5.40
Culture media
Frozen materials
Preserved materials
Skeletons
Microscopes
Wall charts
Stains and Indicators
Seeds
Books
Chemistry Models
Anatomical Models
Earth Science
Environmental
Forensic Equipment
and lots more on our website
www.southernbiological.com
Immediately on receipt of your Stentor culture loosen the cap
and place the culture in a dark cupboard.
Stentor is a single celled motile ciliate, member of the SubKingdom Protozoa, Phylum Ciliophora, Order Heterotnohida,
Family Stentoridae.
Measuring approximately 0.1-0.3 mm in size, Stentor are
barely visible to the naked eye. Morphology varies from
species to species, but the organisms generally are ciliated,
rounded at the rear, with a prominent peristome (mouth).
Typical organelles include a beaded macronucleus, a waterexpelling vesicle, and numerous food vesicles - these
organelles are not readily visible in wet preparations of live
specimens. A thin bluish-green pellicle (membrane) also
surrounds the organism and this is the reason for the greyblue colour of the cultures.
MICROSCOPIC EXAMINATION
We assess our own cultures by examining them in petri dishes
under a stereo-microscope, using 10x or 20x magnification, a
white-plate background, and bright natural light. Look for
pale blue oval or trumpet shaped, organisms.
If you use a compound (monocular) microscope with
transmitted electric light, under 40x magnification, the
Stentor can look dark grey/black in colour,which is totally
different to the image when using incident daylight.
CULTURE MEDIUM
Stentor can be readily maintained in clean glass petri dishes
set in a dark cupboard at room temperature (18-23°C). The
culture medium can be aged pond/stream water (nonpolluted), or pre-made Stentor Culture Medium (CM11),
supplemented with 4-5 grains of pasteurized wheat. Small
ciliated Chilomonas are also normally present in these
cultures and act as a food source for the larger Stentor, and
approx. 2ml of this food supply needs to be added once a
week for the Stentor to flourish.
Supporting Products for
Stentor:
Prepared Microscope Slides
Code: PMS 13.60 Stentor
Posters
Code: WC32 Pond I, Smaller
Microlife
Petri Dishes
Code: E 4.30 Glass Circular
Petri Dish (10)
Code: E 4.10 Plastic Circular
Petri Dish (20)
Ref: L 5.40
23/3/09
1/44 Rushdale Street, Knoxfield 3180 or PO Box 9227,
Scoresby Victoria
3179,
Australia
Phone: +61 3 9753 3896 Fax: +61 3 9753 3959 Email: [email protected]
A Division of Cogitamus Pty Ltd
ACN 097 468 954 ABN 25 838 926 933