Triple Science case study Biology Name of centre Bodmin College: a Specialist Science College Name of teacher Katie Block Subject Biology Specification Edexcel Brief description of case study Students use practical and enquiry skills to collect data in order to find out whether starch is always found in leaves. Disclaimer It is important that the content of this case study is your own work and cannot be attributed to any other source (eg a published scheme of work). Please confirm that this is your own work by signing below. Signed: K Block Date: 26 September 2008 Page 1 of 9 Topic Range and content Storage of starch in plants and the use of starch Æ glucose in respiration How Science Works 1.2 Practical and enquiry skills Pupils should be taught to: a plan to test a scientific idea, answer a scientific question, or solve a scientific problem b collect data from primary or secondary sources, including using ICT sources and tools c work accurately and safely, individually and with others, when collecting first-hand data Extract from Key stage 4 programme of study: http://curriculum.qca.org.uk/key-stages-3-and4/subjects/science/keystage4/index.aspx Context of the lesson within scheme of learning In previous lessons students investigated plant cells and photosynthesis to make predictions about where starch may be found in a plant. They drew and labelled diagrams and considered the function of all of the parts. In this lesson students use their knowledge to make a prediction about starch and where it might be found in plants, and then carry out an investigation to answer their question. Learning objectives • Apply knowledge about plant cells and photosynthesis to predict where starch may be found in a plant. • Work safely and accurately to obtain reliable results to answer the question ‘Where will starch be found in plants?’ Learning outcomes Students will be able to: • reflect on their knowledge of plant cells, photosynthesis and respiration to predict where starch will be found in a plant • work safely to gain accurate results about where starch is present • discuss and develop an explanation for their results. Page 2 of 9 Key concepts/knowledge/skills/understanding addressed by this lesson Plants photosynthesise to produce glucose, which is converted into starch. Plants kept in the dark de-starch, in order to use glucose for respiration. Plants kept in the light should have starch present in their leaves. Safe use of practical equipment. Curriculum opportunities X a Research, experiment, discuss and develop arguments. b Pursue an independent enquiry into an aspect of science of personal interest. c Use real-life examples as a basis for finding out about science. d Study science in local, national and global contexts, and appreciate the connections between these. e Experience science outside the school environment, including in the workplace, where possible. f Use creativity and innovation in science, and appreciate their importance in enterprise. g Recognise the importance of sustainability in scientific and technological developments. h Explore contemporary and historical scientific developments and how they have been communicated. Other curriculum opportunity from specification (give details): Extract from Key stage 3 programme of study: http://curriculum.qca.org.uk/key-stages-3-and4/subjects/science/keystage3/index.aspx?return=/key-stages-3-and-4/subjects/science/index.aspx Page 3 of 9 How Science Works progression in this lesson The aspect of How Science Works being developed is: • Enquiry skills: collecting first-hand data to answer a scientific question • Using investigative approaches: assessing risk and working safely How are students making progress? • This practical includes the use of ethanol and naked flames, and is potentially dangerous. • Students are asked to use and apply risk assessments in carrying out this practical. They will include an explanation about how the risks have been identified and controlled in their recording of the practical. Assessment opportunities in this lesson An understanding of photosynthesis and respiration will be assessed via questioning, particularly when students discover no starch in the leaves of the plant that has been kept in the dark. Practical skills in risk assessment, working safely and accuracy of working can be assessed throughout the investigation. How ICT is used to support learning A data projector is used to display information during the practical to support students. Overview of lesson Lesson title: Kept in the dark Approximate length of lesson: 80–85 minutes Starter Individual activity: Choose 10 foods, link food back to the plant from which it originated, identify the main nutrient and state the place of origin Main 1 In small groups students create a concept map of the uses of plants and discuss 2 Introduce the question ‘Do you expect plant leaves to contain starch?’ Discuss. Students predict where they will find starch present 3 Introduce the practical. Students carry out a risk assessment 4 Students complete the practical Plenary Page 4 of 9 Discuss findings and answer the original question. Students suggest reasons for their results Lesson in detail Lesson title Kept in the dark Lesson structure Learning episode and timing Detail Starter (10 minutes) Project partially filled simple table on to the whiteboard. For example: Food Plant Nutrient (mainly) Place of origin Peanut butter Peanut Fat/protein Brazil Chips Starch UK Potato Students should complete the table for 10 foods, identifying the plant the food is linked to, what the main nutrient is that it contains, and where the plant comes from. Main 1 (10 minutes) Students should work as small groups to categorise the ways in which plants are used and construct a concept map. The ideas are discussed and shared as a class. Main 2 (10–15 minutes) Introduce the make-up of plants by a series of questions. • What is made in photosynthesis and how do we know? • What do plants use for energy and how do we know? • Which parts of the plant are most likely to have starch present? Introduce the key question: ‘Do you expect plant leaves to contain starch?’ Discuss in pairs and then in groups of four. Feed back to class. Ask the students to make a justified prediction about what they will find, referring to ideas about respiration and photosynthesis. Page 5 of 9 Main 3 (10 minutes) Introduce the practical work. Ask students to carry out a risk assessment of the practical before they begin.* • Students should set up a water bath and then turn off the gas once the water is boiling. • Place a leaf from the plant kept in the light into the boiling water for 1 minute. • Remove the leaf and dry it using paper towel. Then place the leaf in a boiling tube with ethanol. Leave the leaf in the boiling tube with the ethanol in the water bath for 10 minutes. • Remove the leaf and rinse it in the warm water. Dry the leaf using paper towel, place it on a tile and add a few drops of iodine solution. • Repeat for a leaf from a plant that has been kept in the dark for 24–48 hours. *It is essential that students do not use ethanol when there are naked flames in the room. I suggest that the teacher keeps the ethanol and does not distribute it until all flames are extinguished, as different groups are likely to work at different rates. Main 4 (30 minutes) Students complete the practical investigation, considering safety as they work. During the 10-minute wait for the leaves to soak, students draw a diagram in their books and annotate it with details of what is happening. For example, ‘The boiling water helps to break down the plant cell walls; the ethanol causes the chlorophyll to be extracted from the leaf.’ When the practical is complete, students draw diagrams of the leaf from the plant that was kept in the light and the one from the de-starched plant. Plenary (10 minutes) Page 6 of 9 Discuss whether the students’ findings are consistent with their prediction. In the light of the evidence collected in this practical, students are asked to write an explanation of their results, using key ideas about photosynthesis and respiration. Reflection and evaluation Starter Students enjoyed completing the table by choosing the foods they ate regularly and linking them to plants. There was some discussion and reflection on where their food comes from. Students seemed knowledgeable about what types of foods were produced locally. Main Main 1: The concept mapping was a useful activity to find out what students knew already and it generated further discussion about plants and why they are so important. Students carried out discussions in small groups. There were many different ideas and these were shared with the whole class. Main 2: The introduction of the main concept of the lesson provided the students with the opportunity to reflect on what they could remember about photosynthesis and respiration. Questions about starch and leaves were generated by students and other students tried to answer them. Students all thought that the leaves would contain starch, and few predicted that the leaves in the dark would be different. We did not discuss this very much; students tended to construct fairly ‘shallow’ explanations about why they predicted that all the leaves would contain starch, and struggled to make the links between photosynthesis and respiration that could affect the result. Main 3: The practical investigation was introduced. Students were already familiar with the apparatus. I chose to use an enquiry inductive approach through this practical work to help to reinforce the point about how photosynthesis and respiration are inter-related. I felt that this would help the students to remember it and really develop their thinking. Main 4: The students dealt effectively with the practical and were clear about how to minimise the risks. It was useful for them to have a task to complete during the ‘waiting time’, and there were useful conversations during this time as some students began to revise their thinking in the light of the results obtained. Plenary The discussion following the practical was the most valuable. It gave the students a chance to reflect on their findings and try to come up with a conclusion that matched the results they had obtained. We discussed why there was no starch found in the leaves that had been in the dark, and all students could explain that there could have been no photosynthesis in these leaves. Most students could go further and explain that respiration was still occurring in the leaves of the plant stored in the dark and that the starch would have been used up by this process. Some students suggested investigations to explore this further. One example was to investigate the amount of starch in leaves from plants that were stored in the dark for differing amounts of time between 2 and 24 hours, and to find out where in the leaf the starch was used from first. Page 7 of 9 Risk assessment Main hazards identified Who would be affected? Control measures to reduce risk Ethanol near naked flames Students and staff No naked flames while the ethanol is being used Ethanol or iodine in eye Students Wear eye protection Broken glass Students Take care when using glassware Report any breakages immediately Burns from Bunsen burner or hot water Students Take care and use a test tube holder for test tubes Use tweezers to remove the leaves from the hot water Opportunities to take this further Students will go on to develop their understanding and skill at collecting and interpreting primary evidence further by devising a series of experiments to investigate limiting factors in photosynthesis. Page 8 of 9 Resources Data projector Two plants (we used geraniums), one kept in the light and one kept in a cupboard for 24–48 hours. You may need more plants, depending on the size of the groups that will work with a leaf Ethanol Glassware Tweezers Test tube holders Stopclocks Bunsen burners Iodine White tiles Dropping pipettes Paper towels A further investigation into starch in variegated leaves can be found on the Science and Plants for Schools website at www-saps.plantsci.cam.ac.uk/worksheets/activ/prac5.htm Bibliography National Strategies Framework for Teaching Secondary Science: Overview and Learning Objectives (2008) www.standards.dcsf.gov.uk/secondary/frameworks Science Programme of Study for KS3 (Extract from National Curriculum) (QCA 2008) http://curriculum.qca.org.uk/key-stages-3-and4/subjects/science/keystage3/index.aspx?return=/key-stages-3-and4/subjects/science/index.aspx Science Programme of Study for KS4 (QCA 2008) http://curriculum.qca.org.uk/keystages-3-and-4/subjects/science/keystage4/index.aspx Page 9 of 9
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