Natural Selection at Work Brigitte Steinmetz John Adams Middle School Santa Monica – Malibu Unified Charles Darwin Famous Naturalist – Did many things… things… Discovery of the Galapagos Finches during a 5 year voyage on the H.M.S. Beagle (1831(1831-1836). 1809 -1882 http://anthro.palomar.edu/evolve/evolve_2.htm - 8/24/2004 Darwin’s Observations Identified 13 species of finches on the islands, but only one on the mainland! Each species had a beak that differed slightly from the other. http://anthro.palomar.edu/evolve/evolve_2.htm - 8/24/2004 1 What do you think? Make a hypothesis as to why you think the Galapagos Finches had different types of beaks? http://anthro.palomar.edu/evolve/evolve_2.htm - 8/24/2004 Predator Prey Lab Predator (5 morphs) Prey (5 morphs) Knife Spoon Fork Forceps Hand Green Black Brown Red Green Environment – Green grass next to Volleyball courts. Predator Prey Lab Rules Utensil = mouth Cup = stomach 40 seconds to feed per round Grab food with utensil and place into cup. NO SCOOPING! GOOD SPORTSMANSHIP – no knocking cups over or utensils! No hunting after hours! Stop on teacher’ teacher’s signal. 2 What do you think? Which predator do you hypothesize will be the most successful? Least successful? Which prey do you hypothesize will be the most successful? Least successful? Let’s try to prove our hypothesis wrong! Post Lab Generation vs. # of Surving Predators 3.5 Students graph their data. 3 # of Surviving Predators 2.5 Hand 2 Spoon Forceps Fork 1.5 Knife 1 0.5 Generation vs. # of Surviving Prey 0 450 Generation 1 Generation 2 Generation 3 Generation 400 350 # of Surviving Prey 300 White 250 Red Brow n Black 200 Green 150 100 50 Students answer analysis/conclusion questions. 0 Generation 1 Generation 2 Generation 3 Generation Concepts Individuals in a species are different in that their characteristics will be varied from one another; happens randomly via genetic mutations. Some characteristics help the individual survive and reproduce (adaptation (adaptation)) passing on those traits to the next generation (natural selection). selection). 3 Tying Everything Together In addition to beak differences, Darwin noticed that the environment (food source) for each bird was different. His conclusion… conclusion…mainland finches adapted to new environment – those that had the best beak for a particular environment survived/reproduced making more offspring with their characteristics. Those that didn’ didn’t died! We have NATURAL SELECTION! Peppered Moth Example Today – Environmental controls in place (less pollution); Lichen growing back; abundance of moths light in color increasing. 1895 – 98% of moths were dark. Pre – Industrial Revolution Post – Industrial Revolution Lichen on trees. No lichen (killed by soot) on trees. http://anthro.palomar.edu/evolve/evolve_2.htm - 8/24/2004 4 Predator Prey Lab Lesson Plan Objectives: Students will be able to explain how natural selection leads to evolution. CA State Standards: Middle School Life Science Standards: 3a-b, 4f, 7c, 7e High School Biology Standards: 7a-d Materials: Predators: 5 morphs (your choice) – some examples = fork, spoon, hand, forceps, knife Prey: 5 morphs (your choice – some examples = different colored beans, different colored string Mouths: cups (1 per student) Stopwatch Calculator (optional) Plan: - - - This lab can be done in the classroom and outside of the classroom. Divide the students into 5 equal groups. Assign each group a particular predator. Spread out the prey on the feeding ground (i.e. classroom floor, grassy area). Explain the following rules to students: o They will have 40 seconds to feed. Students must not begin early. o They must grab the food using their utensil and place the food in the cup with the utensil. No SCOOPING. o They must show good sportsmanship. Knocking other students’ cups over or utensils is grounds for disqualification. o No hunting after hours. Students must stop on teacher signal. Optional: Introduce a secondary predator during feeding time. These secondary predators hunt the primary predators. Upon tagging individual they must empty contents of cup (stomach) into secondary predator’s cup and leave the feeding ground. When the feeding time is up, have students stop and count the number of each type of prey they caught. They should write down these numbers on their data sheet. Ask each student for their total prey caught for each type. Add all students’ totals together for each type of prey. Fill in the data chart on the board. Have the students help you calculate and fill in the chart on the board. Calculate the following with the students help: o Total prey killed by each predator o Average kills per predator o # of surviving predators o total prey killed for each type o number of surviving prey o population of next prey generation Brigitte Steinmetz (John Adams Middle School, Santa Monica – Malibu Unified School District) Page 1 8/15/2004 - - - Determine the next generation of predators based on the number of students who went extinct. The successful predators will reproduce and their offspring will be the students who became extinct in the first round. For example, if 8 students became extinct then you will want to assign these 8 students a new type of predator (the offspring). Repeat the above steps for a total of 3-4 rounds. Back in the classroom, graph the results. You could graph the following: o Predators: Generation (x-axis) vs. # of individuals (y-axis) o Prey: Generation (x-axis) vs. # of individuals (y-axis) o Predators: Type of predator (x-axis) vs. Total # of prey killed (y-axis) o Prey: Type of prey (x-axis) vs. Total # of prey survived (y-axis) Ask students to analyze the graph. Possible questions: o Which type of predator was the most successful? Why? o Which type of prey had the best survival rate? Why? o What factors do you think could change the outcome of this experiment? o What characteristics made it difficult for predators to feed? Easy? o What characteristics made it easy for prey to survive? Difficult? References/Resources: Internet: http://www.csun.edu/science/ - Loads of information and links to several other sites. http://www.pbs.org/wgbh/evolution/ - PBS website http://www.bbc.co.uk/education/darwin/index.shtml - BBC education http://anthro.palomar.edu/evolve/evolve_2.htm http://anthro.palomar.edu/synthetic/synth_7.htm http://www.mc.maricopa.edu/~reffland/anthropology/origins/asm97.html Books: Prentice Hall Life Science Text Book, Prentice Hall Inc., New Jersey, 2001. Laboratory Manual: Biological Sciences 112/113 (Spring 1998). University of Southern California Department of Biological Sciences, 1995. Brigitte Steinmetz (John Adams Middle School, Santa Monica – Malibu Unified School District) Page 2 8/15/2004 PREDATOR AND PREY (From Biological Sciences 112/113 Laboratory Manual (Spring 1998), University of Southern California) Purpose: 1. To study a simulation of a predator-prey relationship between two populations each with a considerable amount of variation. 2. To study what is meant by natural selection. 3. To explain why changing environments change population characteristics. 4. To show what is meant by "the pressures of the predator populations 'mold' prey populations." 5. To demonstrate how the prey populations also "mold" the predator populations. 6. To explain why variation within a species is the "raw material" of natural selection. Materials: Predators: 5 morphs -fork, spoon, knife, forceps, and hand Prey: 5 morphs -black, brown, white, red, green beans Mouths: Styrofoam cups Stopwatch Clipboard and pencil Calculator optional Introduction: Variations occur spontaneously within a species in a given population. Certain individuals in any species are better equipped or adapted in some ways to obtain food, escape predators, or physiologically utilize food more efficiently. Some members of a species are more successful than others in building nests or attracting mates or in the case of flowering plants, in attracting pollinators. The individuals with characteristics that increase their capacity to obtain nutrients, escape predators, withstand adverse climatic conditions, or attract mates, will have more of a chance of surviving and reproducing. Inheritable variations that give any organism improved chances of surviving until reproductively mature and then improve chances of leaving viable offspring are called adaptations. In this exercise, the predators and the prey have selective forces on each other and therefore each influences the survival of the other. Well adapted species will survive and reproduce and the poorly adapted species will die, perhaps without leaving any offspring. The students are the predators using the 5 aforementioned morphs of feeding tools to identify your particular species. The prey are the 5 morphs of beans which are scattered in the environment (grass). The predators who capture less than the mean number of prey captured are considered extinct (natural selection at work). Some of the remaining students whose species become extinct will be reborn as another species with a different feeding mechanism, representing the offspring of the successful predators of the previous generation. The number of surviving prey are calculated and doubled for the next generation. The hunt continues for up to four generations if time permits. Procedure: a. Divide the students into 5 equal groups. Example: 15 students divide into 5 groups with 3 students in each. b. Give all the students in each group a particular predator. Ex: 3 students get a fork, 3 a knife, 3 a pair of forceps and 3 use their hands. Also give each student a cup. c. Count out 100 beans of each color. You can save time by doing this before lab. d. Arm the T.A. with a watch with a second hand or a stopwatch. e. Line the students up around the periphery of the area to be the "hunting grounds". Use an area of lawn (or artificial grass) around 10’ x 10’. f. Mix the beans and spread them out on the ground. g. Tell the students to start and time them. Give them approximately 40 seconds to gather a good amount of beans. h. The students should pick up the beans with their utensil and drop the beans into their cups (mouths). They shouldn't shovel or scoop the beans into the cup. A reasonable amount of jostling among the students is considered normal competition and is to be expected. Knocking other students' utensils or upsetting their cups (making them vomit) is not normal competition and should be discouraged! i. When time is up, have the students stop; discourage hunting after hours. j. Have the students count the number of beans of each color (and the total number of beans.) Have the students tell the T .A. how many beans they have. The T .A. should keep track of the beans on the score sheet. When all the students have reported their bean counts the T.A. totals the number of beans of each color that each type of predator “ate." Then total the number of each color of bean that survived, that is, (total beans of each color) - (number of beans captured) = number of survivors. Ex: If 23 green beans are captured in generation 1, 100 -23 = 77 green survivors. k. Have the surviving beans "reproduce" by adding 1 bean to the population for every surviving bean of each morph. Ex: As in the above example, 77 green beans survived so 77 new green beans are added to the population for a total of 154 green beans in generation 2. Keep track of the populations; you need the numbers to calculate survivorship in the following rounds. Get the students to count out the new beans that you remembered to bring with you for reproduction purposes. l. Calculate the average number of beans captured per predator. Add the total number of beans captured and divide by the number of predators. m. Announce the average (round off to the nearest whole bean.) Any predator who had less than the average becomes extinct. Have these students turn in his/her utensil. n. Allow the "successful" predators to reproduce proportionally (which isn't always easy with 15 -20 students.) Ex. 1: 4 hands, 3 forceps and 1 spoon survived. There are 8 students who just became extinct and can be used as the reproduced predators. Hand out 4 new hands, 3 new forceps and 1 new spoon. Certain morphs will become extinct rather quickly. In either case, keep track of the new total of each predator morph. o. Do a second round by repeating steps h -n. p. Try to go 4 rounds so that distinct trends will emerge. Certain predators will do well while others will become extinct. Beans generally won't become extinct although some will do better than others. In the lawn, the green beans will of course do better than the other morphs. Artificial grass can give different results. If the beans are the same size, shape and texture, color will be the important factor in survival. Lentils have an extra advantage as do peas (flat and round respectively). Pinto beans might do well in dirt. q. Graphs should be generated for the post lab. First Generation A. Predator survivorship Number of prey killed per individual predator, number of surviving predators, and population of next generation. White Number of Prey Killed Red Brown Black Green Total Prey Killed Average kills per individual # of Surviving Predators Population of next generation Population Hand Spoon Forceps Fork Knife B. Prey Survivorship Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green Population Total # Killed Number of Survivors Population of next generation Second Generation A. Predator survivorship Number of prey killed per individual predator, by prey type. White Population Hand Spoon Forceps Fork Knife Number of Prey Killed Red Brown Black Green Total Prey Killed Average kills per individual # of Surviving Predators Population of next generation B. Prey Survivorship Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green Population Total # Killed Number of Survivors Population of next generation Third Generation A. Predator survivorship Number of prey killed per individual predator, by prey type. White Number of Prey Killed Red Brown Black Green Total Prey Killed Average kills per individual # of Surviving Predators Population of next generation Population Hand Spoon Forceps Fork Knife B. Prey Survivorship Number of prey killed, number of survivors, and population of next generation, by prey type. White Population Total # Killed Number of Survivors Population of next generation Red Brown Black Green Addendum to Predator and Prey Lab First Generation A. Predator survivorship Number of prey killed per individual predator, number of surviving predators, and population of next generation. Number of participating students = 25 (5 for each type of predator). Below is an example of a chart I would use to collect data from the students (on board). Explanation of table: Total # of prey killed is total number of each type of prey killed by all individuals. Average kills per individual = Total Prey Killed ÷ # of predators For Hand: 91 total prey killed ÷ 5 hand predators = 18.2 (we round to nearest whole # to get 18) Total Average kills per individual for all = Added avg. kills per individual (18+20+13+8+4 = 63) ÷ total# of types of predators (5); so, 63 ÷ 5 = 12.6…nearest whole # = 13. Predators who had less than 13 average kills go extinct. For this example, we will say that 1 Hand, 1 Spoon, 2 Forceps, 3 Forks, 4 Knives went extinct. If this is the case, then the following # of survivors are: 4 Hands, 4 Spoons, 3 Forceps, 2 Forks, 1 Knife. To get the population of the next generation we include the surviving predators + their offspring (to keep the students involved we reproduce proportionately). For example, in this case 11 students became extinct. So, we need to assign those students offspring. For this example, I will assign 4 new hands, 4 new spoons, 2 forceps, 1 fork. I explain that the knife cannot have an offspring because only one survived. There must be a minimum of two survivors to mate. Population White Number of Prey Killed Red Brown Black Green Total Prey Killed Hand 18 21 22 20 10 91 Average kills per individual 18 Spoon 21 18 23 22 15 99 20 4 8 Forceps 16 11 17 15 8 67 13 3 5 Fork 12 9 6 10 2 39 8 2 3 Knife 4 5 3 5 2 19 4 1 1 # of Surviving Predators 4 Population of next generation 8 B. Prey Survivorship Number of prey killed, number of survivors, and population of next generation, by prey type. For this example, I used 100 of each color of bean. Total # Killed = Total # killed by ALL predators # of Survivors = Population – Total # Killed Population of next generation = 2 x # of survivors Population White 100 Red 100 Brown 100 Black 100 Green 100 Total # Killed 71 64 71 72 37 Number of Survivors Population of next generation 29 36 29 28 63 58 72 58 56 126 Brigitte Steinmetz (John Adams Middle School, Santa Monica-Malibu Unified School District) Page 1 8/15/2004 Predator Prey Lab Background: Some individuals in an ecosystem and species are better at surviving in their environment. Those that are survive long enough to have babies. The characteristics that make them better at surviving are passed on to the next generation (the next set of babies). Investigation: Adaptations and Predator-Prey interactions. Purpose: To discover the characteristics which help predators and prey survive the best in their environment. Materials: Prey (beans) – white, red, brown, black, green. Predator – Hand, Spoon, Forceps, Fork, Knife Data: First Generation A. Predator Survivorship (Ind = individual data; Grp = Group data) Number of prey killed per individual predator, number of surviving predators, and population of next generation. Number of Prey Killed (individual & group data) White Ind Grp Red Ind Grp Brown Ind Grp Black Ind Green Grp Ind Grp Total Prey Killed Group Data Average # of kills per Surviving individual Predators #Population of next generation Population Hand Spoon Forceps Fork Knife B. Prey Survivorship (group data) Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green # Population Total # Killed Number of Survivors Population of next generation Second Generation (with secondary predator introduced): A. Predator Survivorship (Ind = individual data; Grp = Group data) Number of prey killed per individual predator, by prey type. Number of Prey Killed (individual & group data) White Ind Population Hand Spoon Forceps Fork Knife Grp Red Ind Grp Brown Ind Grp Black Ind Grp Green Ind Grp Total Prey Killed Group Data Average # of kills per Surviving individual Predators #Population of next generation B. Prey Survivorship (group data) Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green Population Total # Killed Number of Survivors Population of next generation Third Generation A. Predator Survivorship (Ind = individual data; Grp = Group data) Number of prey killed per individual predator, by prey type. Number of Prey Killed (individual & group data) White Ind Grp Red Ind Grp Brown Ind Grp Black Ind Green Grp Ind Grp Total Prey Killed Group Data Average # of kills per Surviving individual Predators #Population of next generation Population Hand Spoon Forceps Fork Knife B. Prey Survivorship (group data) Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green Population Total # Killed Number of Survivors Population of next generation Analysis/Conclusion Questions (answer questions on a separate piece of paper): 1) As a class, graph your data (each student should do this on graph paper) showing the number of survivors for each type of predator and each type of prey for all three generations. There should be two graphs (one for predators and one for prey). 2) Which predator was better at surviving (had the most # of survivors)? Predators not good at surviving? 3) What characteristics made it hard for the predators to feed on the prey? Easy? 4) Which prey was better at surviving (had the most # of survivors)? Prey not good at surviving? 5) What characteristics made it hard for the prey to survive? Easy? 6) What happened when a certain type of predator or prey survived? 7) What happened when a certain type of predator or prey didn’t get enough food or got eaten and died? 8) What changed the number of predator survivors in the second round? 9) List at least two other things that we could do that would change the number of surviving predators and prey. Predator Prey Lab Answer Key from SDAIE science 2002-2003. Background: Some individuals in an ecosystem and species are better at surviving in their environment. Those that are survive long enough to have babies. The characteristics that make them better at surviving are passed on to the next generation (the next set of babies). Investigation: Adaptations and Predator-Prey interactions. Purpose: To discover the characteristics which help predators and prey survive the best in their environment. Materials: Prey (beans) – white, red, brown, black, green. Predator – Hand, Spoon, Forceps, Fork, Knife Data: First Generation A. Predator Survivorship (Ind = individual data; Grp = Group data) Number of prey killed per individual predator, number of surviving predators, and population of next generation. Number of Prey Killed (individual & group data) White Ind Ind 23 8 26 12 12 Hand Population Grp Red Spoon Forceps Fork Knife Grp Brown Ind Grp 2 10 0 9 14 Black Ind 6 0 3 1 0 Grp Total Prey Killed 0 0 0 0 0 34 19 29 23 27 Green Grp Ind 3 1 0 1 1 Group Data # of Average Surviving kills per individual Predators 17 10 10 8 9 1 1 2 1 0 #Population of next generation 2 2 4 2 0 B. Prey Survivorship (group data) Number of prey killed, number of survivors, and population of next generation, by prey type. White # Population Total # Killed (population – killed) Number of Survivors Population of next generation (survivors x 2) Brown Black Green 100 100 Red 100 100 100 81 26 10 6 0 19 74 90 94 100 38 148 180 188 200 Second Generation (with secondary predator introduced): A. Predator Survivorship (Ind = individual data; Grp = Group data) Number of prey killed per individual predator, by prey type. Number of Prey Killed (individual & group data) White Population Ind Hand Spoon Forceps Fork Grp 9 0 15 5 Red Ind Grp 8 0 2 10 Brown Ind Grp 8 0 0 2 Black Ind Grp 3 0 0 0 Grp Total Prey Killed 0 0 0 0 48 0 17 17 Green Ind Group Data Average # of kills per Surviving individual Predators 24 0 4 9 1 0 2 1 #Population of next generation 3 0 6 4 0 Knife 0 0 0 0 0 0 0 0 B. Prey Survivorship (group data) Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green Population 38 148 180 188 200 Total # Killed 29 20 10 3 0 Number of Survivors (population – killed) Population of next generation (survivors x 2) 8 128 170 185 200 16 256 340 370 400 Third Generation A. Predator Survivorship (Ind = individual data; Grp = Group data) Number of prey killed per individual predator, by prey type. Number of Prey Killed (individual & group data) White Ind Population Hand Spoon Forceps Fork Knife Grp 6 0 14 2 0 Red Ind Grp Brown Ind 31 0 33 8 0 Grp Black Ind 12 0 12 4 0 Grp Total Prey Killed 2 0 2 14 0 63 0 68 36 0 Green Grp Ind 12 0 7 8 0 Group Data # of Average Surviving kills per individual Predators 21 0 12 12 0 #Population of next generation 1 0 3 2 0 B. Prey Survivorship (group data) Number of prey killed, number of survivors, and population of next generation, by prey type. White Red Brown Black Green Population 16 256 340 370 400 Total # Killed 22 72 28 27 18 Number of Survivors Population of next generation 0 184 312 343 382 Analysis/Conclusion Questions (answer questions on a separate piece of paper): 1) As a class, graph your data (each student should do this on graph paper) showing the number of survivors for each type of predator and each type of prey for all three generations. There should be two graphs (one for predators and one for prey). 2) Which predator was better at surviving (had the most # of survivors)? Forceps Predators not good a surviving? Knife. 3) What characteristics made it hard for the predators to feed on the prey? Camouflage – green been was in the green grass. Size was small. Didn’t have anything to catch and keep the bean in its mouth. Easy? Size – big. Other colors (i.e. white) were easy to see and so the predator could catch them. Hand, forceps had more grip and could hold onto the bean. 4) Which prey was better at surviving (had the most # of survivors)? Green. Prey not good at surviving? White 5) What characteristics made it hard for the prey to survive? Different colors than the grass. Easy? Green grass is same color as green bean. 6) What happened when a certain type of predator or prey survived? They reproduced (made babies). 7) What happened when a certain type of predator or prey didn’t get enough food or got eaten and died? Can’t reproduce…they can’t make more babies for next generation. 8) What changed the number of predator survivors in the second round? 2nd predator that ate the 1st predator. 9) List at least two other things that we could do that would change the number of surviving predators and prey. 1) less of one species and more of the others. 2) change the prey’s color. 3) change the environment.
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