runview
NEW
For combs and
accessories,
see page 8
Base unit with
integrated power supply
runVIEW is an innovative new system which can
be used to maximise the efficiency of DNA
recovery from runSAFE, EtBr and SYBR stained
gels by minimising the number of steps involved
in post electrophoretic purification. runVIEW
consists of an multiSUB CHOICE gel chamber with special
bluVIEW lid, containing an orange spectral emission filter
within its viewing pane, plus a base unit with integrated
power supply and blue LED gel illuminator.
Simple DNA recovery
Using runVIEW for gel-free DNA recovery is simple:
1. Cast a gel with two identical rows of wells using one of
the matching pairs of 3mm combs supplied. Transfer to
the tank, remove the combs, and add buffer just to cover
the gel and fill the wells. Load DNA (‘loading tier’).
2. Replace the lid and apply the power. Turn on the blue
LED and see the DNA samples migrate in real-time.
3. When the DNA bands of interest enter the second row of
wells (‘extraction tier’), stop the power, remove lid and
harvest the DNA by pipette.
Once the DNA bands of interest enter the ‘extraction’ tier,
simply stop the power supply, remove the lid and harvest
the DNA by pipette.
Extraction fan
ensures clear
viewing area,
free from
condensation
runVIEW tank
with bluVIEW lid
Track DNA without harmful UV
Biocom “run”
Blue light is safe and has none of the detrimental effects of
UV transillumination, including mutagenesis which can
compromise cloning efficiency, while runVIEW’s capacity to
provide real-time visualisation of electrophoresis enables
DNA to be tracked as it migrates through the gel. This
allows the user to judge precisely when the band of interest
is ready for extraction.
series represents
Save on time-consuming gel elution techniques
Running DNA into a well containing buffer effectively
purifies the DNA of agarose, eliminating the need for timeconsuming gel excision and purification techniques that
also result in sample loss. Once harvested, the DNA needs
only to be cleaned by a straightforward ethanol precipitation
when it is ready for further digestion or ligation.
No expensive commercial gels
runVIEW works with standard EtBr, SYBR Green and SYBR
Safe gels cast within the 15x7, 15x10 or 15x15cm CHOICE
gel trays, and therefore does not require expensive precast
gels and accessories.
a safer
alternative to the
use of UV
irradiation and
ethidium
bromide, both of
which are known
to have harmful
mutagenic
effects.
A self-contained system
The base unit, which houses the in-built power supply and
blue LED gel illuminator, is compact, dual-voltage and
portable, and allows electrophoresis, gel visualisation and
extraction to be performed at the bench, without the
inconvenience of having to transport gels to a darkroom
elsewhere within the laboratory.
REAL-TIME GEL ELECTROPHORESIS
Blue LED gel
illuminator
Typical Applications
Real-time size fractionation and
recovery of nucleic acids
runVIEW and runDOC,
see pages 16, 17
15
REAL-TIME GEL ELECTROPHORESIS
runview
• Power supply integrated
within the base unit –
adjustable in precise 1V or 1mA
increments to a maximum 150V
or 300mA constant voltage or
current output; timer function to
999 minutes for extended runs
• Specialist combs for
specialist applications double-sided 1mm preparatory
combs (1-/2-sample and 4-/16sample standard) included for
nucleic acid recovery, plus four
multichannel compatible 20/28-sample combs for rapid
screening of nucleic acids from
96-well thermal cycler blocks
and microtitre plates. Extra
thick 3mm preparatory combs
also included for enhanced DNA
recovery
• Upgrade to real-time existing MSCHOICE users may
customise their system by
purchasing the base station and
bluVIEW lid
• Versatile - power supply and
gel illuminator may be used with
other gels and electrophoresis
systems
After gel pouring and sample loading, simply lift and position the runVIEW tank
with bluVIEW lid within the base unit
• COMPREHENSIVE –
INCLUDES EVERYTHING*
REQUIRED TO PERFORM
HORIZONTAL REAL-TIME GEL
ELECTROPHORESIS
• CONVENIENCE –
COMBINES
THE HIGH RESOLUTION
CAPABILITY WITHIN A SINGLE
COMPACT BENCH TOP UNIT
• VISIBILITY –
EXTRACTOR
FAN IN LID ELIMINATES
CONDENSATION, TO ALLOW
• OPTIONAL
GEL
DOCUMENTATION SYSTEM
FITS DIRECTLY OVER THE
BASE UNIT AND GEL TANK
FOR IMAGING AT THE END
OF THE ELECTROPHORESIS
RUN
• VALUE –
COSTS
30-50%
LESS THAN INDIVIDUAL
COMPONENTS; GEL TANK,
POWER SUPPLY AND
TRANSILLUMINATOR
REAL-TIME OBSERVATION OF
BAND MOVEMENT
runVIEW Viewing Hood
Technical Specifications
runVIEW Viewing Base Unit
Blue Light Wavelength
470nm
Voltage / Resolution
25-150V / 1V
Current / Resolution
300mA / 1mA
Power
30W
Operating Mode
Constant Voltage or Current
Timer
Safety Device
Operating Temperature
Dimensions
Rated Voltage
1-999 minutes with alarm
No load detection
Ambient to 40°C
29 x 22 x 8cm
100-240V, 50/60Hz
runVIEW Gel System
Gel Dimensions (W x L)
runVIEW Lid Design
15 x 7, 15 x 10 & 15 x 15cm
VLID1 - bluVIEW lid - bluVIEW lid – Amber
(runSAFE & SYBR stains); Built-in extractor fan powered
by base unit
Unit Dimensions (W x D x H)
Combs
500ml
Comb Thickness
26.5 x 17.5 x 9cm
2 x extra thick 3mm preparatory combs double sided,
4 & 16MC and also 20/28MC.
2 x 1mm doubled sided combs, 4 & 16MC and 4 x double
sided combs 20 & 28MC
1mm, 3mm
Buffer volume
ORDERING INFORMATION
RVMSCHOICE7
RVMSCHOICE10
RVMSCHOICE15
RVMSCHOICETRIO
RVSTATION
RVBSBVLID
RVLID1
RVLID2
RVHOOD
16
runVIEW System complete with 15 x 7cm gel tray, one set of casting dams and 8 combs
runVIEW System complete with 15 x 10cm gel tray, one set of casting dams and 8 combs
runVIEW System complete with 15 x 15cm gel tray, one set of casting dams and 8 combs
runVIEW System complete with 15x7cm, 15x10 & 15x15 gel trays, one set of casting dams and 8 combs
runSTATION complete with RVGELDOC and RVMSCHOICETRIO
MS15-1/2-1
Comb 1 & 2 sample, 1mm thick
runVIEW Base Station & bluVIEW Lid
MS15-4/10MC-1
Comb 4 & 10MC sample, 1mm thick
runVIEW bluVIEW lid - Orange (EtBr), optional
runVIEW bluVIEW lid - Amber (runSAFE), standard
MS15-20/28MC-1 Comb 20 & 28MC sample, 1mm thick
MS15-4/2M-3
Comb 4 sample 2 Marker, 3mm thick
runVIEW Viewing Hood
MS15-6/2M-3
Comb 6 sample 2 Marker, 3mm thick
*except chemicals and reagents
GEL DOCUMENTATION
runDOC
runDOC is a portable, lightweight gel documentation system designed
exclusively for use with runVIEW. runDOC comprises a darkroom
hood and a high resolution digital camera to capture images of
nucleic acid gels stained with EtBr, SYBR and runSAFE. Its
12.1 megapixel camera with 2GB memory card allows
storage of images in RAW and JPEG formats.
Hood is positioned over the
runVIEW tank and base unit
to create a light-tight
environment suitable for
image capture
The runDOC darkroom is placed quickly and easily
over the runVIEW gel tank located on the runVIEW
base unit, which includes an integrated power supply
and blue LED gel illuminator. The blue LED
illuminator provides the excitation source for the DNA
gel located within the tank, and following excitation the
light emitted is then visualised using either one of the
bluVIEW lid options (supplied and used with the runVIEW
gel tank) or the runDOC filter slide. The interchangeable
filter slide and bluVIEW filter options offer full flexibility:
amber filter for runSAFE, SYBR and green fluorescence;
and orange filter for EtBr and red fluorescence.
runDOC may be also used like an ordinary gel documentation
system - by placing the gel tray directly on the runVIEW base station, which
acts as a transilluminator. runDOC is available as a complete system with laptop and software.
NE W
runSAFE
• runSAFE – general purpose stain for DNA ranging
from 50bp markers to large super-coiled plasmid
• runSAFE-PLUS500 – for DNA larger than 500bp in size;
CAMERA
TYPE
5X OPTICAL / 4X DIGITAL ZOOM
12.1MP
EFFECTIVE PIXELS
640X480 UP TO 4000X3000
IMAGE SIZE
SENSOR
1/1.7” TYPE HIGH SENSITIVITY CMOS
RAW, JPEG
FILE FORMAT
COMPUTER INTERFACE USB 2.0 HI-SPEED (MINI- B-JACK)
VIDEO OUT
NTSC / PAL
8GB SD MEMORY CARD
STORAGE MEDIA
LENS
6.1-30.5 MM
FOCAL LENGTH
MAXIMUM APERTURE F/1.8(W)-F/2.9(T)
15-1/4000S
SHUTTER SPEED
EMISSION FILTER OPTIONS
ORANGE FILTER FOR ETBR;
RUNDOC FILTER SLIDE
AMBER FILTER FOR SYBR AND RUNSAFE
BLUVIEW-ORANGE FOR ETBR;
BLUVIEW LID OPTIONS* BLUVIEW-AMBER FOR SYBR
AND RUNSAFE
DARK ROOM
CAMERA HOLDER MATERIAL POLYOXYMETHYLENE (POM)
DARKROOM MATERIAL EBONY ACRYLIC
DIMENSIONS / WEIGHT 40X34X19.5CM (HXWXD) / 0.8KG
POWER
RECHARGEABLE LI-ION BATTERY AND
PLUG-IN CHARGER
OPTIONAL MAINS CABLE CHARGER
*ONLY WITH RUNVIEW
runSAFE Range - Less DNA damage, improved cloning efficiency
Plasmid
PCR
Products
• SAFE –
ALL FOUR RUNSAFE
STAINS HAVE ULTRA-LOW TOXICITY
(LC>5000MG/KG) AND LACK
CELL PERMEABILITY
10000
1000
• CONVENIENT –
CFU
The runSAFE Range comprises four non-mutagenic
stains that produce instant visualisation of DNA
bands upon irradiation with blue light or UV in
agarose or polyacrylamide gels. Each stain is
conveniently supplied in a 6x loading dye which is
mixed with 5 parts double-stranded DNA before
loading onto an agarose or polyacrylamide gel. The
individual colour constituents within each loading dye
track electrophoresis run progression, and indicate the
extent of band separation and resolution by comigrating with DNA bands of known molecular size,
which varies according to the gel percentage. All four
stains are non-toxic, safe for the environment and can
be disposed of in the regular laboratory waste without
using expensive decontamination methods. Each stain
is sensitive and binds DNA to detect as little as 0.2ng
DNA per band within a gel; while gel imaging is best
performed using the amber emission filter found of the
bluVIEW lid or runDOC filter slide. The runSAFE Range
comprises the following:
Technical Specifications
100
Blue LED System
10
0
15
30
45
Exposure Time (sec)
60
120
Slower migrating species, indicative of a linear or relaxed circular vector, resulting from
DNA nicking or strand breaks, are significantly reduced in DNA plasmid mixed with runSAFE and exposed to blue light. The concentration of nicked DNA plasmid increases
significantly after 8’ of exposure to EtBR and UV irradiation.
• runSAFE-2000 – for DNA vectors and inserts ranging from 500-2000bp;
• runSAFE-500 – for small DNA fragments, PCR products, sequence tracts
and primers less than 500bp.
EACH STAIN IS
SUPPLIED AS A READY TO USE 6X
LOADING DYE; SIMPLY ADD 1 PART
STAIN TO 5 PARTS DNA, MIX AND
LOAD YOUR GEL
• FAST – NO TIME-CONSUMING
POST-STAINING OR DE- STAINING
OF GELS IS REQUIRED.
• SENSITIVE –
VERY LOW
BACKGROUND STAINING OF THE
GEL; DETECTS AS LITTLE AS 0.2NG
DNA PER BAND
• FLEXIBLE –
USED WITH
EACH STAIN MAY BE
OR UV LIGHT
BLUE
ORDERING INFORMATION
RVGELDOC-F2
Amber Filter for runDOC (runSAFE and SYBR stains)
runVIEW Gel Documentation Hood with 12.1MP camera
CAMCHARGER
Optional Mains Charger Cable for 12.1MP camera
runVIEW Gel Documentation Hood with camera, laptop
RVSTATION
runSTATION complete with RVGELDOC and RVMSCHOICETRIO
& 1D Analysis Software
RVGELDOC-F1
Orange Filter for runDOC (Ethidium Bromide)
RVGDCOMPLETE
runVIEW Package including RVGELDOCSYS and RVMSCHOICETRIO
runSAFE
Description
Tracking Dyes
Size Range
RUNSAFE
runSAFE Stain, 1ml
Bromophenol Blue, Xylene Cyanol FF, Orange G
50bp – 20Kb
RUNSAFE-PLUS500 runSAFE-PLUS500 Stain, 1ml
Bromophenol Blue, Xylene Cyanol Blue
>500bp
RUNSAFE-2000
runSAFE- 2000, 1ml
Xylene Cyanol Blue, Orange G
500-2000bp
RUNSAFE- 00
runSAFE-500, 1ml
Orange G
<500bp
RVGELDOC
RVGELDOCSYS
17