MagNA Pure 96 System
Nucleic Acid Purification with Real High Throughput
The MagNA Pure 96 System purifies DNA, RNA, and viral nucleic acids from
a wide range of starting materials using proven magnetic glass particle technology.
Preprogrammed software protocols are tailored to your sample requirements.
Resulting nucleic acids are ideal for all possible downstream applications.
This industry-first solution for nucleic acid sample purification, the most
demanding step in gene detection and quantification studies, effortlessly processes
up to 96 samples in less than one hour using barcoded, prefilled trays with
ready-to-use reagents. Experience seamless data management using intuitive
features, such as the link to a laboratory information management system (LIMS).
MagNA Pure 96 System
4
MagNA Pure 96 Instrument 5
MagNA Pure 96 Software
6
MagNA Pure 96 Reagent Kits
8
System
Instrument
Software
Reagent Kits
Kits and Applications 10
Applications
Ordering Information 14
Ordering
Information
2
Discover Real High Throughput
Treat all your samples like urgent samples
The MagNA Pure 96 System takes nucleic acid purification to a new dimension:
J
Speed
Increase your productivity and process up to 96 samples fully automated in less than one hour – including instrument setup.
J
Versatility
Be flexible with the isolation of high quality DNA, RNA or viral nucleic acids from a broad variety
of sample materials and volumes of up to 1.0 ml.
J
Performance
Rely on our more than 10 year expertise in the development and application of magnetic-particle based
nucleic acid purification systems.
J
Convenience
Reduce hands on time by using just a few barcoded, prefilled reagent trays and disposables.
Save time with intuitive software and optimized, preprogrammed purification protocols.
J
Connectivity
Manage data easily linked to a networked file server or LIMS. Ensure data integrity by avoiding
redundant data handling.
Visit us at www.magnapure96.roche.com
to learn more!
3
MagNA Pure 96 System
Discover what high throughput is all about
Treat all your samples like urgent
samples
Process 1 to 96 standard volume samples in less
than one hour including the handling time that is
needed for the setup of a run. Larger volumes
require typically up to 80 to 90 minutes for a run.
The MagNA Pure 96 Instrument uses two
pipetting arms, handling all 96 samples absolutely
standardized at the same time.
System
Convenient instrument loading using
racks and automated vortexing
The few barcoded, prefilled ready-to-use reagent
trays and consumables are loaded into racks for
increased ease and user safety. Simply use a
second set of racks to exchange reagent trays and
purify cellular RNA after isolating DNA.
The onboard magnetic glass particle (MGP) shaker
eliminates manual MGP vortexing.
G Figure 1: A reagent rack in the process of being
loaded with reagent trays
Barcode-supported reagent and
consumables verification
Barcode reading is integrated throughout the entire
purification procedure using a barcode scanner,
minimizing the risk of user errors during loading
and enabling full process documentation.
Flexible elution formats
Purified DNA, RNA or viral nucleic acids are eluted
into the actively cooled MagNA Pure 96 Output
Plate. A passively cooled custom plate can also be
selected for the 96-format, e.g., for archiving or
further downstream use.
4
G Figure 2: Example of a reagent tray containing a
unique barcode identifier
MagNA Pure 96 Instrument
Discover how effortless nucleic acid isolation can be
Advanced process and user safety
Exceptional process safety during sample
preparation is guaranteed by the following
features:
liquid presence sensor
drop protection device
specially designed processing cartridge
integrated UV lamp
Roche offers installation and operational qualification (IQ/OQ) services for the MagNA Pure 96
Instrument.
Instrument
The MagNA Pure 96 Instrument is designed
and manufactured according to the strict regulations
of the Underwriter Laboratories (UL) for product
safety, thus providing a maximum certainty to avoid
any potential harm to users.
Type
Standalone tabletop instrument
Hardware footprint
dimensions
137 cm W x 80 cm D x 100 cm H
(53.9 in W x 31.5 in D x 39.4 in H)
Weight
approx. 235 kg/518 lbs (instrument)
Power supply
100 to 125 V (-15%, +10%)
200 to 240 V (-15%, +10%)
Sample number
1 to 96 reactions per run
Sample volumes
50 to 500 µl with 96 reactions per run
1000 µl with 48 reactions per run
Run time
approx. 50 minutes for 200 µl sample volumes
approx. 80-90 minutes for 500 µl sample volumes
Setup time
less than 5 minutes
G Table 1: MagNA Pure 96 Instrument specifications
5
MagNA Pure 96 System
Onboard preprogramming for small and large volumes
Intuitive, easy-to-use software
Run setup is quick and easy, guided by user-friendly
effortless software:
Program a purification run with just a few
mouse clicks.
Future runs can be programmed while the
instrument is in the process of performing a run.
Customize your templates for an entire run or
parts of a run.
During a run, an overview screen clearly displays
the instrument status that can be seen from a
distance.
Programmed runs can be queued to perform
sequential runs.
Button bar
Run summary
Stage status
Software
G Figure 3: Overview screen of the MagNA Pure 96 Software
6
Instrument status
MagNA Pure 96 Software
Workflow continuity with LIMS data management
Intelligent features
Networked data workflow
Automatic rollover of reagents, by which a second
set of reagent trays is automatically used when the
first set is used up in the same run, is an integral
part of the MagNA Pure 96 System. Automated load
check saves time by detecting an error and checking
only the rack(s) that had been corrected by the user.
Discover further intelligent features during a demo
of a MagNA Pure 96 System.
Exchange data bidirectionally with the Laboratory
Information Management System (LIMS) and
share files in *.xls format.
Entire run data files, or selected run data are
configurable outside the MagNA Pure 96
Software environment for loading before the
run is started.
Export selective or complete run-results for easy
post-purification run documentation or transfer
to another instrument in the workflow.
Consistent data workflow from sample to result
maintains data integrity, avoiding redundant
data handling.
Software
Optimized application protocols
Preprogrammed purification protocols with flexible
parameter options eliminate the time-consuming
steps typically required to program a fully optimized
application.
Full traceability and easy data backups
All barcoded parameters are fully traceable with
an available audit track and CFR21.11 compliant.
Take advantage of seamless experimental data
workflow in combination with Roche Instrument
Systems, such as the LightCycler® 480 RealTime PCR System, featuring direct sample name
uploading to LightCycler® Software.
The MagNA Pure 96 System is provided with a
second hard disc for secure backup of sample data
and purification run information.
G Figure 4: The MagNA Pure 96 Instrument together
with a LightCycler ® 480 Instrument
7
MagNA Pure 96 Reagent Kits
Just a few barcoded prefilled trays and consumables
Broad user application options
The precisely engineered kits are designed using
our more than ten year expertise in magnetic
glass particle technology. We have streamlined the
purification process to just the minimum number
of reagents, consumables and steps. Rely on Roche
manufacturing quality that sets standards as a
supplier for the whole industry.
Kit Name
Pack
Size
Target NA
Optimized preprogrammed purification protocols
make it a routine handling samples for high quality,
reproducible nucleic acid yields. Resulting nucleic
acids are ideal for life science research applications
in genomics, gene expression, and for high sensitivity
testing in virology and microbiology.
Sample Material/Volume
Elution
Volume
Up to 5x105 culture cells
Genomic DNA
DNA and Viral NA
Small Volume Kit
100 or 200 µl whole blood
3x192
(Catalog
No. 05467497001)
Viral DNA
and RNA
Genomic DNA
3x48
(Catalog
No. 05467454001) 3x96
3x48
Cellular RNA
Large Volume Kit
Viral DNA and
RNA
Viral DNA and
RNA, bacterial
and fungal DNA
53
DNA Blood ext lys SV
49
Viral NA Universal ext lys SV
OR Viral NA Plasma ext lys SV
47
Pathogen Universal 200
58
Up to 1x10 6 culture cells
DNA Cells LV
80
500 µl whole blood
DNA Blood LV 500
72
Lysed sample from 100 or 200 µl plasma, serum or whole blood
Plasma, serum, whole blood, urine, bronchoalveolar lavage
(BAL), sputum, cerebrospinal fluid (CSF), swabs, stool and
bacterial cultures. Sample Volume 200 µl
Lysed sample from 500 µl whole blood
DNA Blood ext lys LV 500
75
DNA Tissue LV
80
1000 µl whole blood (containing up to 1x107 blood cells)
DNA Blood LV 1000
75
500 µl plasma or serum
Viral NA Universal LV 500 OR
Viral NA Plasma LV 500
77
Lysed sample from 500 µl plasma or serum
Viral NA Universal ext lys LV
500 OR Viral NA Plasma ext
lys LV 500
69
Pathogen Universal 500
90
Pathogen Universal 1000
96
RNA Cells
80
Plasma, serum, whole blood, urine, bronchoalveolar lavage
(BAL), sputum, cerebrospinal fluid (CSF), swabs, stool and
bacterial cultures. Sample Volume 500 µl
100 or 200
50 or 100
Plasma, serum, whole blood, urine, bronchoalveolar lavage
(BAL), cerebrospinal fluid (CSF), swabs, and bacterial cultures.
Sample Volume 1000 µl
3x96
Up to 1x10 6 culture cells
50, 100,
200
3x48
The complete WBC sediment of one Paxgene tube
(2,5 ml Whole Blood)
100 or 200 RNA PaxGene Half Tube LV
(Catalog
3x96
No. 05467535001)
3x48
Total RNA
Half the WBC sediment of one Paxgene tube
(2,5 ml Whole Blood)
Lysed sample from 400 µl whole blood
50 or 100
Lysed sample from 800 µl whole blood
86
RNA PaxGene LV
86
RNA Blood 400
90
RNA Blood 800
92
Up to 20 mg fresh frozen tissue or RNAlater stabilized tissue
50,100,
200
RNA Tissue FF Standard LV
93
1 FFPE tissue section (5-20 µm thickness)
50 or 100
RNA Tissue FFPE LV
87
1x96
G Table 2: Selected applications for the MagNA Pure 96 System. Further applications will be added and are
downloadable from www.magnapure96.roche.com
8
60
55
Up to 25 mg fresh tissue; 3-10 FFPE tissue slices, depending on
DNA content
DNA and Viral NA
Large Volume Kit
55
Viral NA Universal SV OR
Viral NA Plasma SV
50 or 100
3x96
Min.
DNA Blood SV
100 or 200 DNA Tissue SV
100 or 200 µl plasma, serum or whole blood
Viral DNA and
RNA, bacterial
and fungal DNA
Reagent Kits
DNA Cells SV
50 or 100
Lysed sample from 100 or 200 µl whole blood
Up to 10 mg fresh tissue, single FFPE tissue slices (10 µm)
Protocol Name
MagNA Pure 96 Reagent Kits
Experience how easy MagNA Pure 96 Kits are to use
Reagent convenience minimizes
user handling
Experience how easy MagNA Pure 96 Kits are to
use. Simply position the magnetic glass particles
(MGP) and trays prefilled with reagents on the
instrument stage*. MGP vortexing is automated by
the MagNA Pure 96 System’s integrated shaker.
Purification run setup is possible in less than
5 minutes. Reagent trays are automatically pierced by
metal needles of the MagNA Pure 96 Instrument’s
robotic dispensing head.
Sample numbers are selectable from 1 to 96.
Reagent tray reuse is very convenient. Evaporationproof sealing foils are easily applied, and MGP
bottles are self sealing.
Sealed reagent trays can be stored at room temperature (+15 to +25°C) for 2 to 4 weeks, depending on
kit type, for later use. Opened reagent trays remain
stable unsealed on the instrument overnight.
Reagent Kits
* As an exception for maximal shelf life, only
DNase for cellular RNA applications is provided as
a lyophilisate in a bottle.
9
Kits and Applications
MagNA Pure 96 DNA and Viral NA Small Volume Kit
This kit is ideal for isolation of genomic DNA or
viral DNA or RNA from sample volumes of up
to 200 µl. Obtain nucleic acids of the highest quality
for your downstream applications. Typical yields
for genomic DNA purification from whole blood
samples are 5–8 µg and 11.5 µg for 5 x 105
cultured HeLa cells.
Highly intact genomic DNA
H Figure 5: Genomic DNA was isolated from mammalian
whole blood samples with the MagNA Pure 96 DNA and
Viral NA Kit, using the DNA Blood SV Protocol (200 µl sample
volume). Twenty replicates were applied to an agarose gel,
showing that purified genomic DNA is high molecular weight
and intact.
Applications
10
Linearity of recovery
CP
Isolate high quality genomic DNA or
viral DNA and RNA for PCR or Realtime PCR
CP
40
38
36
34
32
30
28
26
24
22
20
10
40
38
36
34
32
30
28
26
24
22
20
10
EBV – Scalability EDTA Plasma
100
1,000
10,000
Spike (copies)
100,000 1,000,000
HAV – Scalability EDTA Plasma
100
1,000
10,000
Spike (copies)
100,000 1,000,000
G Figure 6a and b: Viral DNA and RNA was isolated from
spiked EDTA-plasma (200 µl) in eightfold replicates. Plasma
was spiked with different amounts of DNA Virus (EBV) and
an RNA Virus (Hepatitis A Virus). The recovery of the viral
nucleic acids was checked using an EBV specific PCR and an
HAV specific RT-PCR with the LightCycler ® 480 Instrument.
Kits and Applications
MagNA Pure 96 DNA and Viral NA Large Volume Kit
Isolate high quality genomic DNA or
viral DNA and RNA for PCR or Real-time
PCR of sample volume up to 1000 µl
This kit is recommended for highly sensitive
applications where large sample volumes are used.
Typical yields for genomic DNA purification
from whole blood are 11–17 µg, and 22 µg from
culture cells (106 K-562 cells).
Reproducibility
Prevention of Cross Contamination
In this experiment, 96 plasma samples of
500 µl sample volume were processed on the
MagNA Pure 96 Instrument in an alternating
(checkerboard) pattern in which samples in
one well were spiked with a high concentration of
Parvo B19 plasmid, and the neighboring wells were
not spiked. All isolates were analyzed for the presence
of Parvovirus B19 DNA using the LightCycler® 480
Instrument and a 384-well PCR plate:
Amplification Curves
0.651
Fluorescence (483-640)
Fluorescence (483-640)
Amplification Curves
1.061
0.961
0.861
0.761
0.661
0.561
0.461
0.361
0.261
0.161
0.061
10
20
Cycles
30
40
E Figure 7: Viral DNA (Parvo B19) was isolated from spiked
horse plasma (500µl) in 96-fold replicates. The recovery of
the viral nucleic acids was checked by a Parvo B19 specific
PCR on the LightCycler ® 480 Instrument. All spiked samples
(light blue) gave reproducible signals in the LightCycler ® test.
Negative samples in red, standards in black.
0.551
0.451
0.351
0.251
0.151
0.051
0.001
10
20
Cycles
30
40
G Figure 8a: All spiked samples (red lines) were found to
have a clear positive amplification signal in the LightCycler®
Real-time PCR, and all negative samples showed negative
result (light blue), demonstrating that there is no cross
sample contamination between neighboring wells. Parvo B19
standards are shown in black.
Applications
G Figure 8b: Positive and negative samples (as described
above) were pipetted into the MagNA Pure 96 Processing
Cartridge in an alternating checkerboard-like pattern.
11
Kits and Applications
MagNA Pure 96 Cellular RNA Large Volume Kit
Using our kit for RNA isolation from cellular samples results in purified RNA of the highest quality for
your downstream applications. Typical yields for cultured cells (1x106 K-562 cells) are 8–10 µg.
Integrity
F Figure 9: RNA from 1x106 K-562
28S rRNA
cells was isolated using the MagNA
Pure 96 Cellular RNA Large Volume Kit.
Analysis using agarose gel electrophoresis demonstrates the high integrity and
quality of the isolated nucleic acids.
18S rRNA
Very low levels of contaminating DNA
F Figure 10: RNA from 1x10 6, 1x105,
1x10 4 K-562 cells was isolated using
the MagNA Pure 96 Cellular RNA
Large Volume Kit. RT-PCR analysis
using the LightCycler® 480 Instrument
shows the high purity of isolated RNA
nearly free of contaminating DNA. During
real-time PCR targeting the housekeeping
gene cyclophilin A, only very small traces
of DNA were found in the isolates.
1.2 % –
1.0 % –
0.8 % –
% DNA
Applications
0.6 % –
0.4 % –
0.2 % –
0.0 % –
0
8
10 6 K-562 cells
12
Sample
10 5 K-562 cells
16
24
10 4 K-562 cells
Kits and Applications
MagNA Pure 96 Instrument, Kits, Consumables and Accessories
Download Application Notes and technical information highlighting the versatility of the MagNA Pure 96
System from www.magnapure96.roche.com:
MagNA Pure 96 System Application
Notes:
MagNA Pure 96 System Technical
Information:
High-throughput detection of bacterial, fungal
The New MagNA Pure 96 DNA and Viral NA
and viral nucleic acids in routine microbiological
Kits: Maximum Speed Combined with High
sample types using one generic Pathogen Universal
Recovery.
Protocol on the MagNA Pure 96 System.
MagNA Pure 96 Cellular RNA Kits:
RNA Isolation on the Fast Lane.
Use of MagNA Pure 96 and LightCycler® Systems
for High Throughput Testing of Stool Samples
for enterohemorrhagic E. coli (EHEC) in an
Outbreak.
Efficient Extraction of Viral DNA and RNA from a
Variety of Patient Sample Materials in Routine
Diagnostics using a Single Standardized Protocol
with the MagNA Pure 96 System.
Comparison of Automated MagNA Pure 96 and
Manual PAXgene RNA Isolation from Stabilized
Peripheral Blood Samples for Expression Analysis
of Individuals with Colorectal Cancer.
Use of MagNA Pure 96 and LightCycler® Systems
for High Throughput Testing of Nail Samples for
Dermatophytes Causing Onychomycosis.*
Utility of the MagNA Pure 96 and LightCycler® 480
Systems for Testing of Stool Samples for Toxinproducing Clostridium difficile in Routine Use.*
Applications
*in preparation
13
Ordering Information
MagNA Pure 96 Instrument, Kits, Consumables and Accessories
1. Instrument
Product Name
Cat No.
Package Contents
MagNA Pure 96 Instrument
05 195 322 001
instrument, control unit, software, accessories
Product Name
Cat No.
Kit Contents
MagNA Pure 96
DNA and Viral NA Small Volume Kit
05 467 497 001
3 x 192 purifications
MagNA Pure 96
DNA and Viral NA Large Volume Kit
05 467 454 001
3 x 96 purifications
MagNA Pure 96
Cellular RNA Large Volume Kit
05 467 535 001
3 x 96 purifications
MagNA Pure 96
System Fluid (Internal)
05 467 098 001
2 containers
MagNA Pure 96
System Fluid (External)
05 467 578 001
1 container
MagNA Pure LC
Total Nucleic Acid Isolation Kit –
Lysis Binding Buffer Refill
03 246 779 001
1 bottle
RNA/DNA Stabilization Reagent for
Blood/Bone Marrow
11 934 317 001
500 ml, for up to 50 ml sample material
MagNA Pure Bacteria Lysis Buffer
04 659 180 001
20 ml
Product Name
Cat No.
Contents
MagNA Pure 96
Filter Tips (1000 µl)
05 392 900 001
8 blisters with 5 trays à 96 tips
MagNA Pure 96
Processing Cartridge
05 435 315 001
36
MagNA Pure 96
Sealing Foil
05 435 307 001
100
MagNA Pure 96
Output Plate
05 435 285 001
60
MagNA Pure 96
Internal Control Tube
05 435 293 001
150 (15 x 10)
2. Reagent Kits
3. Consumables
Ordering
Information
14
Ordering Information
MagNA Pure 96 Instrument, Kits, Consumables and Accessories
5. MagNA Pure 96 Related Workflow Products
4. Accessories
Product Name
Cat No.
Contents
MagNA Pure 96 Drop Catcher
05 532 086 001
10
05 449 634 001
1
MagNA Pure 96 Barcode Scanner
05 449 804 001
1
MagNA Pure 96 Tip Rack
05 449 855 001
1
MagNA Pure 96 Bottle Rack
05 449 936 001
1
MagNA Pure 96 Needle Set of 4
05 450 039 001
Set of 4
MagNA Pure 96 Purification Rack
05 450 047 001
1
MagNA Pure 96 Reagent Rack
05 450 055 001
1
MagNA Pure 96 Waste Rack
05 450 063 001
1
05 523 940 001
1
05 523 931 001
1
05 532 124 001
10
05 679 435 001
1
MagNA Pure 96 Reagent
Loop Unit
MagNA Pure 96 External Waste
Container
MagNA Pure 96 Internal Waste
Container
MagNA Pure 96 Waste Cover
MagNA Pure 96 Cooling Block
Custom
MagNA Lyser Instrument
For homogenizing or prelysis of up to 16 tissue samples, optimized for use with MagNA Pure and High Pure nucleic acid
purification reagents
Product Name
Cat No.
Contents
1 MagNA Lyser
Instrument (230 Volt)
03 358 976 001
2 rotors, 1 rotor stand,
1 rotor cooling block
1 MagNA Lyser
Instrument (110 Volt)
03 358 968 001 2 rotors, 1 rotor stand,
1 rotor cooling block
MagNA Lyser
Green Beads
03 358 941 001 100 tubes, pre-filled
with ceramic beads
LightCycler ® 480 Instrument
For real-time PCR with 96 or 384 PCR plates
Product Name
Cat No.
Contents
LightCycler ® 480 Instrument II
(96-well version)
05 015 278 001
1 instrument
LightCycler® 480 Instrument II
(384-well version)
05 015 243 001
1 instrument
Roche Applied Science Service and Support
At Roche Applied Science we are committed to
providing innovative, high-quality instruments and
reagents combined with excellent customer service
offering powerful tools to address the evolving
needs of life science researchers worldwide.
For more information, visit
Whether you need expert technical support, online
access to comprehensive product information,
convenient on-site product supply service and
online ordering, or outstanding customer service to
ensure accurate and timely product delivery, we
provide a wealth of resources to help you achieve
your research goals.
Trademarks and disclaimers:
www.roche-applied-science.com to explore
our products and services or to find a local
representative.
For life science research only.
Not for use in diagnostic procedures.
Ordering
Information
HIGH PURE, MAGNA LYSER, MAGNA PURE and LIGHTCYCLER
are trademarks of Roche.
All other product names and trademarks are the property of their
respective owners.
15
Published by:
Roche Diagnostics GmbH
Sandhofer Straße 116
68305 Mannheim
Germany
© 2012 Roche Diagnostics.
All rights reserved.
www.roche-applied-science.com
05928613001 2 0212