0.10
GC3 (%)
D
92.5
87.5
82.5
77.5
72.5
67.5
62.5
97.5
97.5
92.5
87.5
82.5
77.5
72.5
67.5
0.10
GC3 (%)
97.5
92.5
87.5
82.5
77.5
72.5
67.5
62.5
57.5
52.5
47.5
0
42.5
0.05
37.5
97.5
92.5
87.5
82.5
77.5
72.5
67.5
62.5
57.5
52.5
47.5
42.5
37.5
32.5
0
27.5
0.05
0.15
32.5
0.10
0.20
27.5
0.15
n = 153, GC3 46.4±9.2%
0.25
22.5
Fraction of genes
0.20
Xenopus
0.30
n = 183, GC3 56.9±12.3%
0.25
22.5
Fraction of genes
F
mouse
0.30
57.5
GC3 (%)
GC3 (%)
E
62.5
97.5
92.5
87.5
82.5
77.5
72.5
67.5
62.5
57.5
52.5
47.5
42.5
37.5
32.5
27.5
22.5
0
57.5
0
0.05
52.5
0.05
0.10
47.5
0.10
0.15
42.5
0.15
0.20
37.5
Fraction of genes
0.20
n = 183, GC3 53.9±16.8%
0.25
32.5
n = 175, GC3 46.5±12.6%
0.25
human
0.30
27.5
turtle
0.30
Fraction of genes
GC3 (%)
22.5
C
52.5
47.5
42.5
0.05
0
97.5
92.5
87.5
82.5
77.5
72.5
67.5
62.5
57.5
52.5
47.5
42.5
37.5
32.5
0
27.5
0.05
0.15
37.5
0.10
0.20
32.5
0.15
n = 176, GC3 51.4±13.0%
0.25
27.5
Fraction of genes
0.20
chicken
0.30
n = 175, GC3 49.7±13.0%
0.25
22.5
Fraction of genes
B
anole
0.30
22.5
A
GC3 (%)
Additional file 4. GC3 distribution of the orthologs in other vertebrates. Histograms show
frequency distributions of orthologs in green anole lizard (A), chicken (B), Chinese soft-shelled
turtle (C), human (D), mouse (E) and Xenopus tropicalis (F).