Blue Heron, Your Gene Synthesis Partner
You Design it—We Build it
Simple to Complex Sequences
Codon Optimization
Any species
Variants
Single or pooled clone libraries
Antibody Affinity Optimization
Whole Genome Synthesis
Custom Vector Construction
Blue Heron
Gene Synthesis
A Pioneer and provider of the highest quality and
most comprehensive custom gene synthesis services
to researchers worldwide since 1999.
Gene synthesis provides customers with the most cost
effective and reliable source to obtain genes that are
not already available from cloned libraries. It also
allows researchers to build novel variant libraries or
entire genomes that can lead to new discoveries for
the protein, antibody discovery, synthetic biology and
biofuels industries.
By working with Blue Heron you save money and
valuable time by leaving the synthesis work to
the experts. You receive all your genes — from the
simplest to the most complex — in your vector of
choice, on time, and with no surprises.
Unlike approaches that rely on adaptations of
standard PCR, our GeneMaker® platforms include
exclusive design software that considers multiple
characteristic of each section of the target DNA
sequence, and optimally builds each section using
multiple, proprietary process technologies. This way,
Blue Heron successfully completes projects where our
competitors' processes often fail.
Researchers from the top 20 pharmaceutical and
biotechnology companies, as well as emerging
synthetic biology and biofuels companies like The
Venter Institute; choose Blue Heron for our unmatched
technological capability, quality of customer service,
and long record of success and industry leadership.
RESEARCH BENEFITS
n Get
any gene or synthetic DNA sequence
codon optimization- any species
n Expression vector cloning
n Secure and confidential data transfer
n No claims to your IP
n Free
QUALITY
n Automated
ordering process to minimize errors
technology platforms
n 2x sequence verified data confirmation
n Technical customer service
n Unmatched
TECHNOLOGY LEADER
PARTNERS IN DISCOVERY
n 52kb
length gene delivered
megabase a month capacity
n First synthetic genome partnership
n Most complex sequences synthesized
n First
RESEARCH ARTICLE
Creation of a Bacterial Cell Controlled by a Chemically
Synthesized Genome
Daniel G. Gibson1, John I. Glass1, Carole Lartigue1, Vladimir N. Noskov1,
Ray-Yuan Chuang1, Mikkel A. Algire1, Gwynedd A. Benders2, Michael
G. Montague1, Li Ma1, Monzia M. Moodie1, Chuck Merryman1, Sanjay
Vashee1, Radha Krishnakumar1, Nacyra Assad-Garcia1, Cynthia
Andrews-Pfannkoch1, Evgeniya A. Denisova1, Lei Young1, Zhi-Qing
Qi1, Thomas H. Segall-Shapiro1, Christopher H. Calvey1, Prashanth
P. Parmar1, Clyde A. Hutchison III2, Hamilton O. Smith2 and J. Craig
Venter1,2,*
Science 329, 52-56. July, 2010.
www.blueheronbio.com
UNMATCHED SERVICE
n Confidentiality
guaranteed
time delivery
n Customer service ready to help
n Automated quoting tools
n Quotes to orders with a simple click
n On
Cloning Services
Once a gene or synthetic DNA fragment has been
synthesized, Blue Heron offers standard vector as
well as custom expression vector cloning. Using
our custom expression vector services provides you
with a finished, expression ready product for your
downstream projects.
RESEARCH BENEFITS
n Cloned
Plasmid Prep Services
MIDI AND MAXI-PREP
n Ion
exchange column purified
transfection-grade DNA
n Sequence verified
n Receive the quantity you need
• 100ug
• 500ug
• 1000ug
n Receive
and ready for the next step
verified in the final vector
n Sequence
APPLICATIONS
n Protein
expression
n Antibody development
n shRNA or rare RNA template
n Transgenics studies
CUSTOM VECTOR DESIGN
n Reshuffled
vector sites
vector
n Specialized cassette drop-in vectors
n Custom designed vectors
n Multi-resistance
ORIGENE EXPRESSION VECTORS
n Mammalian
expression
n E.coli expression
n Epitope and fluorescent tags
425-368-5000
Generating DiversitySynthetic Variants
Antibody Engineering to
Increase Affinity
Antigen
Creating diversity through random mutagenesis,
recombination or degenerate oligo methods can be
cumbersome as well as highly inaccurate. Using Blue
Heron’s gene synthesis technology to create synthetic
variants enables a researcher the design flexibility
and guarantee of sequence needed.
Complementary
Determining
Region (CDR)
Antigen-binding site
VH
Heavy Chain (H)
VL
CH
CL
Light Chain (L)
C = Constant Region
V = Constant Region
BENEFITS
n Study
multiple variants in parallel
ready for protein expression
n Available as individual clones or as a pool
n High quality pools decrease downstream
screening costs
n Receive
QUALITY
n Sequence
verified
n Cost/time line defined
APPLICATIONS
n Mutation
analysis
discovery
n Gene/protein function studies types
n Simple to complex base variants
n Amino acid substitutions
n Domain swaps
n Heavily mutated promoter regions
n Fusion or tagged proteins
n Knockout constructs
n Deletions/insertions
n Alternative splice forms
n Antibody
PROCESS
n You
submit the full CDR plus variable region
DNA sequence and the expression vector.
n We create a custom vector containing the
constant regions specific for your project.
n We build the variants, insert the variable region
cassettes into the custom vector.
n Deliverable: Full-length, sequence-verified,
individual antibody clones in your expression
vector.
ADVANTAGES
n Flexibility
to make any amino acid change
within the variable region cassette.
n Delivery reliability
n Faster than using traditional mutagenesis
methods
Cassette Variant Design
New Vector Containing
Constant Region(s)
Constant Region 1
Constant Region 2
Variant 1
Variant 2
Variant 3
Variant 4
Your variants in the New Vector containing constant region(s)
www.blueheronbio.com
Codon Optimization- Free Tool
Blue Heron provides customers with a free Codon
Optimization tool to assist in sequence design. In
many cases researchers have found improved protein
expression results after optimization (see below).
Each optimization request generates an optimized
sequence posted electronically to the customer's
dedicated, secure website.
RESEARCH BENEFITS
n Improve
protein expression
expression improvement
n Remove specific DNA sequences or motifs
n Diminish complexity at the DNA level
n Heterologous
LGR4 AND LGR6 PROTEIN EXPRESSION
IMPROVED USING BLUE HERON'S CODON
OPTIMIZATION ALGORITHM
3 4
1 2
116
116
66
66
45
45
35
LGR4
35
25
25
18
14
18
14
LGR6
Lane 1: construct with a wild type LGR4 DNA sequence
Lane 2: construct with optimized LGR4 DNA sequence
Lane 3: construct with a wild type LGR6 DNA sequence
Lane 4: construct with optimized LGR6 DNA sequence
Protocol: LGR4 and LGR6 proteins were codon optimized using Blue Heron’s
proprietary algorithm. Wild type and optimized sequences were cloned separately
into OriGene’s spCMV-Entry vector. Each DNA was transiently transfected to
HEK293T cells and incubated for 72 hrs. Proteins were purified using anti-DDK
affinitive column and run on SDS PAGE, then stained with Coomassie Blue.
Abbreviation (3 Letter)
Full Name
Codon options
Alanine
Ala
GCT
GCC
GCA
GCG
Arginine
Arg
CGT
CGC
CGA
CGG
Asparagine
Asn
AAT
AAC
Aspartate
Asp
GAT
GAC
Cysteine
Cys
TGT
TGC
Glutamate
Glu
GAA
GAG
CAA
CAG
Glycine
Gly
GGT
GGC
GGA
GGG
Histidine
His
CAT
CAC
Isoleucine
Ile
ATT
ATC
ATA
Leucine
Leu
TTA
TTG
CTT
CTC
Lysine
Lys
AAA
AAG
Methionine
Met
ATG
Phenylalanine
Phe
TTT
TTC
Proline
Pro
CCT
CCC
CCA
CCG
Serine
Ser
TCT
TCC
TCA
TCG
Threonine
Thr
ACT
ACC
ACA
ACG
Tryptophan
Trp
TGG
Tyrosine
Tyr
TAT
TAC
Valine
Val
GTT
GTC
GTA
GTG
AGA
AGG
CTA
CTG
AGT
AGC
425-368-5000
Automated Design Tools
n Project
design tool—NEW!
n Automated quote generation
n Automated quotes to orders—NEW!
n Free codon optimization & design
n Secure, confidential ordering web site
n Set up expression vectors for cloning
n Choose to have a midi or maxi prep prepared
n Sequence and trace data automatically posted
DELIVERABLES:
n 3-5
μg purified plasmid DNA
Bacterial strain containing the plasmid
n Sequencing data and trace data posted
electronically to your secure, dedicated
online account
PROJECT DESIGN TOOL
Ordering is fast and simple:
Step 1
Step 2
Step 3
Enter the DNA Sequence you need
Choose your vector (Standard or Expression)
Check your shipping/billing details
DONE!
n
www.blueheronbio.com
PROJECT VERIFICATION PAGE
Molecular and Protein Biology Solutions
TrueORF/Gold
Human/mouse
Expression vectors
5,000 humn
Custom purification
TrueMAB
TruePlex
Clones/genes
Proteins
Antibodies
Assays
Any species
Any sequence
Any variant
Optimized
expression
Antibody engineering
As of August, 2010, Blue Heron became a wholly
owned subsidiary of OriGene Technologies, Inc.
Combining OriGene’s complete collection of human
cDNA clones with Blue Heron’s gene synthesis
capacity, we can provide a whole product solution for
the molecular biology research community. We are
100% committed to serving our customers with high
quality, strict confidentiality standards, and improved
business efficiency
GENE EXPRESSION
TissueScan cancer qPCR arrays
wide qPCR primer pairs
n qPCR template standards
n microRNA qPCR detection system
n
n Genome
PROTEINS AND ANTIBODIES
n
n
OriGene Product Line
n
n
5,000 purified human proteins
Heavy-labeled proteins as MS standard
12,000 transfected over-expression lysates
TrueMAB™ monoclonal antibodies
CDNA CLONES: OVER-EXPRESSION
n TrueORF®:
tagged cDNA clones
gold clones: expression validated
n Over 50 organelle markers
n TrueClone®: native protein expression
n microRNA expression plasmids
n TrueORF®
RNAi: DOWN-REGULATION
n Pre-designed
HuSH-29 shRNA
human, mouse and rat genes
n Guaranteed knock down
n microRNA expression plasmids
n Every
425-368-5000
OriGene, Your Partner in Gene Research and Beyond
KEY TECHNOLOGIES AND PRODUCTS
n Full-length
cDNA clones, ORF clones in expression-ready vectors
n Gene
synthesis: any gene, any variant, any vector
n RNAi
research reagents: shRNA, siRNA, and miRNA function and detection
n SYBR
Green qPCR assays for mRNA and miRNA detection, primer panels
n Recombinant
n TrueMAB™
n Luminex
n Cancer
human proteins
monoclonal antibodies
multiplex immunoassays
tissue biorepository, TMA, RPPA, and TissueScan qPCR arrays
Blue Heron-Gene Synthesis
Headquarters:
Bothell, WA USA
Contact Us: www.blueheronbio.com
1-425-368-5000
Sales:
[email protected]
Customer Service:
[email protected]
OriGene Technologies, Inc.:
Rockville, MD USA
Contact Us: www.origene.com
1-301-340-3188
1-888-267-4436 (U.S. only)
[email protected]
[email protected]