Honey as a Biomolecule Name _________________________ Part 1: Introduction Inordertostayhealthy,peopleneedtoeatavarietyofnutritiousfoods.Foodcontainssixnutrients thatarenecessaryforgoodhealth.Thesenutrientsincludecarbohydrates,proteins,lipids, minerals,vitamins,andwater. Carbohydrates,lipids,andproteinsarecalledorganicmolecules.Thesemoleculesarecalledorganic moleculesbecausetheyarecarbonbasedandshouldnotbeconfusedwithcertifiedorganic products.TheUSDAdefinesorganicagricultureasusingmethodsthatpreservetheenvironment andavoidmostsyntheticmaterialaspesticidesandantibiotics. Readeachstatementandchecktheappropriateboxindicatingifyouthinkthatthestatementis trueorfalse. True False Statement Ateaspoonofhoneyandateaspoonofsugarhavethesamenumberof calories. Ateaspoonofhoneyandateaspoonofsugarhavethesamenumberof carbohydrates. Ateaspoonofhoneyandateaspoonofsugarhavethesameeffectona person’sglycemicindex(GI).GIisameasureofhowcarbohydrates affectaperson’sbloodsugarlevel.HighGIfoods“spike”aperson’s bloodsugarlevelwhichmayincreaseaperson’sriskofcoronaryheart diseaseandType2diabetes. Honeyisaddedtocertainbakedgoodstoextendshelflife. Honeyisnotrecommendedforinfantsunderoneyearofage. Divideintogroupsof2-3students.Readthefollowingarticles.Discusswithyourgroupifyou learnedanythingnewabouthoneyortablesugar. • “CarbohydratesandtheSweetnessofHoney” http://www.honey.com/images/downloads/carb.pdf • “IsHoneytheSameasSugar?” http://extension.arizona.edu/sites/extension.arizona.edu/files/pubs/az1577.pdf 1 Honey as a Biomolecule (continued) Part 2: Comparing Carbs Carbohydratesarethemainsourceofenergyforlivingorganisms.Glucose,fructose,andgalactose arethethreemonosaccharidesorsinglesugarmolecules.Disaccharidesareformedbytwo monosaccharidesbondedtogetherandincludesucrose,alsoknownastablesugar,andlactose,the sugarfoundinmilk.Polysaccharidesarelargemoleculesformedbymanymonosaccharidesbonded together.Animalsstorecarbohydratesinthepolysaccharideglycogen.Plantsstorecarbohydrates inthepolysaccharides,starchandincellulose,whichisusedforstructureandsupport.Whether simpleorcomplex,allcarbohydratesaremadeofsugar,butnotallsugarsareequallysweet. Foodscientistshaveratedtherelativesweetnessofcarbohydratesasseeninthetable. RelativeSweetnessScale Carbohydrate Glucose Rating 70-80 Galactose 35 Fructose 140 Sucrose(glucose+fructose) 100 Lactose(glucose+galactose) 20 HighFructoseCornSyrup 120-160 1. Whichmonosaccharideisthesweetest? Digestion:Whenadisaccharidebreaksdownintoitscomponentmonosaccharides,thesweetness changesaswell.Theresultingsweetnesscanbedeterminedbyaveragingthesweetnessofthetwo monosaccharides. Lactoseisthedisaccharidefoundinmilk.Beforelactosecanbeusedbythebody,itmustbebroken downintomonosaccharides.Theenzyme,lactase,breaksdownlactoseintothemonosaccharides, glucoseandgalactose.Whenlactoseisdigesteditssweetnesschanges,too.Thenewsweetnesscan becalculatedbyaveragingthesweetnessratingofthetwomonosaccharides;glucose(75)and galactose(35).Theaveragevalueforglucoseisshownintheexample. Example: !"#$" % = ''( % = 55 2. Howdoesthesweetnessofmilkcomparetothesweetnessofmilkthathasbeendigestedby lactase? 2 Honey as a Biomolecule (continued) 3. Sucroseortablesugarisalsoadisaccharide.Whattwomonosaccharidescomposesucrose? 4. Usingtheaveragevalueforglucose,calculatethesweetnessofsucroseafterithasbeen brokendownintoitscomponentmonosaccharides. 5. Howdoesthesweetnessofsucrosecomparetothesweetnessofsucrosethathasbeen digestedbyenzymes? 6. Breakingdowndisaccharidesintomonosaccharidesisnecessaryfordigestionbutfood scientistsseeanotherbenefit.Ofwhatadvantageisproducingsucrosethathasalreadybeen brokendownbyenzymes? HowBeesProcessNectarintoHoney:Beesmakehoneyby gatheringnectarfromcertainfloweringplants.Nectaris sucrose.Foragingbeescarrythenectartotheirhivewhere receivingbeesunloadthenectarloadandbegintoprocessthe nectarintohoney.Duringtheprocessing,beesaddinvertase,an enzymethatbreaksdownsucrose. 7. Howdoyouthinksweetnessofhoneycomparestothesweetnessofnectar?Explainyour thinking. ExaminetheingredientsonthelabelforJuniorMintstoanswerthequestionsbelow. 8. Whatcarbohydrateshavebeenused? 9. Whatenzymehasbeenaddedandhowwillthataffectthesweetnessofthecarbohydrates? 3 Honey as a Biomolecule (continued) Part 3: Developing a Food Product with Honey Foodscientistsusetheirknowledgeofbiology,chemistry,andchemicalengineeringtobetter understandfoodprocessesandtoimprovefoodproductsforconsumers.Imagineyouareafood scientistdevelopinganewfoodproductusinghoneyasaningredient. 10. Whatisthenameofyourproduct? 11. Whoisthecoreaudienceforyourproduct? 12. Whatwillyourpackaginglooklike? 13. Whatingredientswillbeusedinyourproduct? 14. Drawaquicksketchofwhatyourproductwilllooklike? 15. Listthreereasonswhyafoodscientistmightusehoneyinsteadofsugarinthisrecipe. (Youmaywanttorefertopage4inHoney:AReferenceGuidetoNature’sSweetenerfound here:http://www.honey.com/images/uploads/general/refguide.pdftoreadaboutsomeof theapplicationsforhoneyinfoodproducts.) 4
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