NEW Blue Duo™ Markers
from AMRESCO
Prestained colored markers for
easy protein visualization
Blue Duo™ Protein Marker, Mid-Range
contains 6 colored proteins ranging from
20 to 120 kDa. Blue Duo™ Protein Marker,
High Range contains 6 colored proteins
ranging from 35 to 250 kDa. These proteins
are visualized on a standard SDS-PAGE and can
be seen as the gel is running. Blue Duo™ Protein
Marker contains proteins of two different
colors, so that you can easily determine protein
molecular weight. Blue Duo™ markers are for
use with any SDS-PAGE gel system and can be
transferred to nitrocellulose or PVDF membranes
for Western blotting applications.
AMRESCO's Protein Molecular Weight Markers
AMRESCO offers a broad range of molecular weight markers
specially engineered for protein electrophoresis.
Wide Range,
Protein MW
Marker. 8 bands
ranging from
14.0 to 212.0 kDa.
High Range,
Protein MW
Marker. 5 bands
ranging from 40.0
to 212.0 kDa.
Low Range,
Protein MW
Marker. 6 bands
ranging from 3.5
to 31.0 kDa.
Description
Blue Duo™ Protein Marker, Mid-Range
Sufficient for 50 - 100 lanes.
Blue Duo™ Protein Marker, High Range
Sufficient for 50 - 100 lanes.
Prism Protein Marker
Sufficient for 50 - 100 lanes.
Wide Range, Protein Molecular Weight Marker
Sufficient for 100 - 150 lanes.
High Range, Protein Molecular Weight Marker
Sufficient for 40 lanes.
Sufficient for 160 lanes.
Low Range, Protein Molecular Weight Marker
Sufficient for 40 lanes.
Precise™ Protein Molecular Weight Marker
Sufficient for 40 lanes.
Precise™
Protein MW
Marker. 7 bands
ranging from
15.0 to 150.0 kDa.
Prism Protein
Marker. 6
colored proteins
ranging from
19.0 to 130.0 kDa.
Product
Code
Pack
Size
1B1527-500UL
500 µl
1B1584-500UL
500 µl
1B1349-500UL
500 μl
K494-500UL
500 μl
J449-200UL
J449-0.8ML
K880-200UL
200 μl
0.8 ml
200 μl
J383-200UL
200 μl
TO ORDER: www.amresco-inc.com • Tel 800.448.4442 • Fax 440.349.3255 • [email protected]
Protein EZ-Vision®, 4X
Non-toxic, non-mutagenic protein
visualization dye in loading buffer
Protein Electrophoresis Convenience Products
Product
Code
Pack Size
Protein EZ-Vision®, 4X
Non-hazardous, fluorescent dye that produces instant visualization of protein
bands upon UV illumination of SDS-PAGE gels. Supplied in a 4X loading buffer.
N836-KIT
2 x 1 ml
Laemmli Loading Buffer, 4X
Formulated for use in Laemmli SDS-PAGE.
M337-5ML
5 ml
M337-25ML
25 ml
E270-1ML
1 ml
E270-5ML
5 ml
Protein Gel Loading Buffer, 2X, with Pyronin Y
Contains Pyronin Y. Used for denaturing protein electrophoresis.
M266-5ML
5 ml
Loading Dye Base, 25X
For custom dye preparation.
E271-1ML
1 ml
E271-5ML
5 ml
M217-1ML
1 ml
Description
LOADING BUFFERS & DYES
Protein Gel Loading Buffer, 2X
Contains glycerol and SDS. Used for denaturing protein electrophoresis.
Tracking Dye, 2D
Used for 2D protein electrophoresis.
Protein EZ-Vision® is compatible with
downstream Western Blotting Applications. Protein EZ-Vision® (A) and Coomassie®
Blue (B) staining of protein molecular weight
markers and cellular lysate. A 12.5% Laemmli
SDS-PAGE gel was run using Protein EZ-Vision®,
4X as the sample loading buffer. The following
were loaded in each lane: Lanes 1 and 3-10 µL
of AMRESCO’s Protein Molecular Weight Markers,
Wide Range (K494) Lanes 2 and 4-10 µg of K562
cell lysate. Immediate fluorescent visualization of
protein post-electrophoresis in lanes 1 and 2 was
achieved with a 2 sec UV exposure using a Sybr
Green filter with the Syngene GBox-HR Gel Doc
System (A). Lanes 3 and 4 were post-stained with
Coomassie® Blue protein stain for one hour and
destained one hour (B). AMRESCO’s Rapid Western Blotting Kit was used to detect Vimentin (C).
Proteins from Lanes 1 and 2, which were detected
using Protein EZ-Vision® (A), were transferred
onto a PVDF membrane with a semi-dry transfer unit for 10 minutes in Rapid Transfer Buffer
(N789). The blot was blocked and probed with
anti-vimentin and Rapid Western Blotting Kit.
AMRESCO’s VisiGlo PLUS™ HRP Chemiluminescent
Substrate Kit (N219-KIT) was used for detection.
ACRYLAMIDES
Colored Laemmli Stacking Gel Solution
Premixed acrylamide buffer forms a colored stacking gel for easy sample
loading. Sufficient for 10 gels (50 ml) or 20 gels (100 ml).
Acryl/Bis™ 37.5:1 Solution
40% solution containing 38.96% (w/v) acrylamide and 1.04% (w/v) bisacrylamide for monomer to cross-linker ratio of 37.5:1.
Acryl/Bis™ 37.5:1 Premixed Powder
Blend of ultra-pure acrylamide and bis-acrylamide at a ratio of 37.5:1.
1B1401-50ML
50 ml
1B1401-100ML
100 ml
0254-500ML
500 ml
0907-40G
40 g
0907-200G
200 g
NEXT GEL® POLYACRYLAMIDE GEL SOLUTIONS
NEXT GEL®, 10% Solution
Ready-to-pour SDS polyacrylamide solution allows faster gel casting with no
stacking gel required.
M256-100ML
100 ml
M256-500ML
500 ml
NEXT GEL®, 12.5% Solution
M257-100ML
100 ml
M257-500ML
500 ml
M258-100ML
100 ml
M258-500ML
500 ml
NEXT GEL®, 15% Solution
SPRINT NEXT GEL®, 10% Solution
Ready-to-pour SDS polyacrylamide solution offers reduced electrophoresis
time because gel can be run at high voltage without overheating.
M312-100ML
100 ml
M312-500ML
500 ml
SPRINT NEXT GEL®, 12.5% Solution
M311-100ML
100 ml
M311-500ML
500 ml
M291-500ML-KIT
500 ml
Fluorescent NEXT GEL®, 12.5% Solution
Ready-to-pour SDS polyacrylamide solution with convenient fluorescent dye
stains proteins during electrophoresis. Reliably separates proteins and fully
compatible with 1D and 2D SDS-PAGE and downstream applications. Kit includes
Fluorescent NEXT GEL®, 12.5% Solution and NEXT GEL® Running Buffer, 20X.
TO ORDER: www.amresco-inc.com • Tel 800.448.4442 • Fax 440.349.3255 • [email protected]