BioResearch
Lonza Cells on Demand™ Custom Services
Expand Your Research Options
Custom Cell Services
Let Us Satisfy Your Unique
Research Needs
Lonza is the market leader in primary cell research solutions. Supported by decades of cell isolation and transfection experience, Lonza
has built an extensive catalog offering consisting of quality primary
cells, media, reagents and devices to assist in drug development and
basic research. Cells on Demand™ Custom Services was established to
expand this offering and meet each customer’s unique research applications.
Expand Your Research Options
Modifications to Existing Catalog Products
Cells on Demand™ provides the option to manipulate our standard catalog products to suit your experimental needs. Examples include:
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Additional donor characteristics
Donor-matched cell sets
Pooled donors
Cell expansions
Additional testing
Cell Isolations from Various Tissues
Through our extensive tissue sourcing, Cells on Demand™ goes
beyond our standard offering to provide cell types of interest from a
variety of tissues. Each project adheres to the same ethical sourcing
procedures and quality systems of Clonetics™ and Poietics™ Primary
Cell Systems. We can provide cell isolations from a variety of species
including (but not limited to) human, primate, porcine, canine, feline
and murine tissue. We have successfully isolated many cell types, including, but not limited to:
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Human retinal pericytes
Human renal podocytes
Human renal pericytes
Cynomolgus monkey lung microvascular endothelial cells
Feline and Porcine renal cells
BioResearch – Lonza Cells on Demand™ Custom Services
Podocytes at P7, podocin overlay (10x).
RAFT™ 3D Culture Services
Cell Characterization
Lonza’s patented RAFT™ 3D Culture System is designed to closely mimic the in vivo environment of cells within 96-well, or 24-well plate formats. Utilizing Lonza’s RAFT™ System, we can establish 3D Cell Culture
Systems tailored to your needs, such as:
The Cells on Demand™ Team has a broad experience in many areas of
cell biology. Harness this experience and let us develop a cell-based
assay for your functional testing of recombinant proteins and small
molecules. This can be performed on the recombinant cell lines we
have generated, or a specific primary cell type in either 2D or 3D Cell
Culture Systems. We offer:
– Develop RAFT™ System protocols for your specific cell type
– Generate co-cultures and barrier models
Ratio Firefly / Renilla
– Development of custom cell-based assays with assay optimization and SOP generation
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IC50 = 0.04627 μg/mL
R2 = 0.9931
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Dermal fibroblast fixed and stained after 11 days in RAFT™ System.
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Generation of Recombinant Cells
Cells on Demand™ offers fast and convenient creation of recombinant
cell lines. Due to our focus and extensive experience using difficult and
hard to transfect cell lines, we have successfully generated single-cell
derived clones of cell lines such as sCHO, J774.A1, Jurkat, HL-60, THP1, U937, K562, BJAB, MCF-7, SHSY-5Y and SW480. We can generate
specific cell lines overexpressing your gene of interest also including:
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Clone 24
GFP
Clone 28
Clone 34
Clone 39
Clone 51
RLU
Mean Fluorescence Intensity
– Stably transfected pools and single-cell clone formats
(cryopreserved)
– Low, medium, and/or high level expressing cell lines including
functional characterization in accordance to your requirements
– Data sheets including expression characterization and CoA
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Log Conc. Compound [μg/mL]
Compound treatment of sarcoma cells stably expressing an inducible luciferase reporter construct.
Cell Line Expansion and Banking
Use Lonza as an extension of your cell culture facility. Lonza´s Cells
on Demand™ Team can expand your cell line according to specific standards and cryopreserve the cells in your preferred format. This will allow you to focus on your assays and results. We can provide:
– Expansions of your specific single-cell derived clone or cell line up
to 10e10
– Generation of master cell banks
– Generation of working cell banks
– QA and delivery of CoA
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Luciferase
Jurkat E6-1 cells were stably transfected with GFP and luciferase reporter constructs.
Selected Jurkat E6-1 single-cell derived clones showed diverse co-expression levels of
GFP and luciferase.
Lonza Cells on Demand™ Custom Services – BioResearch
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www.lonza.com/research
www.lonza.com/cellsondemand
Immortalization Services
Modify your primary cell to have cell line proliferative capacity. Cell lines
are a common screening model because they are easily bulked up to
large, homogenous quantities. They often, however, lack the relevant
phenotype of the primary cell. In contrast, senescence of primary cells
can cause undue frustration for researchers. We overcome the limited
lifespan of primary cells with cell immortalization. Lonza has developed
a catalog of Clonetics™ Conditionally Immortalized Cells. If your cell type
of interest is not in our offering, the Cells on Demand™ Team can produce
an immortalized cell line to your requirements. Our offering includes
stable immortalization and conditional immortalization. While a stably
immortalized cell will regain its proliferative capacity, a conditionally immortalized cell can be switched back to the status of a primary cell after
proliferation. We understand that developing these cell lines is not without technical risk, so we have implemented a risk-sharing, milestone approach. On completion of the project we provide a biologically relevant,
quality tested screening model.
To learn more about Cells on Demand™, email cellsondemand@lonza.
com or visit http://www.lonza.com/cellsondemand
Primary dermal fibroblasts were immortalized using hTERT.
Contact Information
North America
International Offices
Customer Service: +1 800 638 8174 (toll free)
[email protected]
Scientific Support: +1 800 521 0390 (toll free)
[email protected]
Australia
Belgium
Brazil
France
Germany
India
Japan
Luxemburg
Singapore
The Netherlands
United Kingdom
Europe
Customer Service: +32 87 321 611
[email protected]
Scientific Support: +32 87 321 611
[email protected]
International
Contact your local Lonza distributor
Customer Service: +1 301 898 7025
Fax:
+1 301 845 8291
[email protected]
+ 61 3 9550 0883
+ 32 87 321 611
+ 55 11 2069 8800
0800 91 19 81 (toll free)
0800 182 52 87 (toll free)
+91 22 4342 4000
+ 81 3 6264 0660
+32 87 321 611
+ 65 6521 4379
0800 022 4525 (toll free)
0808 234 97 88 (toll free)
Lonza Cologne GmbH – 50829 Cologne Germany
For research use only. Not for use in diagnostic procedures.
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and technical knowledge. However, no warranty is made, either
expressed or implied, regarding its accuracy or the results to be
obtained from the use of such information and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. Any user must make
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recommendations given by Lonza Group Ltd or its affiliates are (i)
suitable for intended process or purpose, (ii) in compliance with
environmental, health and safety regulations, and (iii) will not infringe any third party’s intellectual property rights.
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