Farmers inspiring farmers Seasonal soil moisture and nitrogen availability Dr Cassandra Schefe1 and Stephen Lowe2 Riverine Plains Inc Stony Creek Vineyard 1 2 Key points • Soils in the Rutherglen region of Victoria are highly variable. • Most soils across the region increase in clay content in the subsoil, which has a limited capacity to supply water to plant roots. • The distribution of plant-available nitrogen (nitrate-nitrogen) throughout these soils is variable, with some soils having a high concentration of nitrogen (N) near the soil surface, while others store nitrogen at depth. • Sampling for deep soil nitrogen (DSN) by combining all soil from 0–60cm depth does not give a clear picture of where nitrogen is located throughout the profile. Background During June 2015 the North East Catchment Management Authority (NECMA) with funding from the Australian Government’s National Landcare Programme, enabled Riverine Plains Inc to install and monitor soil moisture probes in cropping paddocks at 11 sites across the Rutherglen region of Victoria through the Soil Moisture Probe Network Project. The objective of this project was for growers to understand how knowledge of stored soil moisture can inform their decisions about applying fertiliser. For example, if the soil profile has sufficient moisture, growers might decide to apply enough nitrogen during spring to satisfy the full crop requirement. However, if there is limited stored soil moisture, growers might only apply a smaller amount of fertiliser, as the crop would be entirely dependent on opportune rainfall events to take it through to harvest. In addition, measurements of DSN post-harvest and pre-sowing means the amount of nitrogen mineralised during summer can be accounted for when planning the nitrogen strategy for the following season. The Sustainable Agriculture Victoria: Fast-Tracking Innovation initiative made possible with the support of 66 RESEARCH FOR THE RIVERINE PLAINS 2016 the Foundation for Rural and Regional Renewal (FRRR) together with the William Buckland Foundation allowed the sampling of DSN (broken into incremental depth samples) at each of these sites. By connecting the results from soil nitrogen sampling to soil moisture status, growers can predict if the stored nitrogen will be available to the crop through the year, or if it will be lost through leaching (due to accumulation of nitrogen at depth under high soil moisture conditions). Aim The aim of this project was to increase our understanding of nitrogen availability and movement across, and between, seasons and to appreciate how nitrogen availability is intimately related to soil moisture status. Method Soil moisture probes were installed at 11 sites across the Rutherglen region during June 2015. Probes were removed during harvest (November 2015) and in preparation for sowing (late March 2016). Each probe measured up to four depth intervals (10, 30, 50 and 90cm below the soil surface), with values logged every two hours. The data was manually downloaded from each probe on a regular basis. Gaps in the dataset occurred if the probe was damaged. Deep soil nitrogen sampling was carried out at each of the soil moisture probe locations during June and December 2015, and April 2016 (Figure 1). The June sampling was carried out at a time when many growers across the region undertake DSN sampling to identify how much nitrogen they need to apply to meet crop demand through spring. The December sampling, post-harvest, provided a measure of post-crop residual nitrogen, and the April sampling provided information on the amount of nitrogen lost or mineralised (becoming more plant-available) during the summer months; ready for sowing. Sampling at each of the 11 soil moisture probe sites consisted of one core sample, which was split into increments (0–10, 10–20, 20–30, 30–60 and 60–100cm) before being submitted to the laboratory for analysis of mineral nitrogen (nitrate + ammonium) and total nitrogen (includes organic and inorganic forms — i.e. the total nitrogen soil bank). Corowa Gooramadda Wahgunyah Rutherglen Brimin Boorhaman North Norong Chiltern Valley Chiltern Peechelba East FIGURE 1 Locations of the 11 soil moisture probes installed across the Rutherglen area By measuring both mineral nitrogen and total nitrogen growers can appreciate the role of organic forms of nitrogen in cycling and mineralisation processes. As the nitrogen samples collected were not replicated, they cannot be statistically analysed. As such, the results presented provide an indication of nitrogen availability, however as they are sampled from one point in the paddock, there is the possibility the results are not representative of the rest of the paddock. Results Soil moisture and available nitrogen Please note the graphs included in this section have not been plotted on a common axis, so take care when comparing the values between sites. The laboratory results for the ammonium fraction were highly variable and potentially unreliable. As such, they have been excluded from the analysis, with just the nitrate-nitrogen fraction of mineral nitrogen being presented. The monthly rainfall data for Rutherglen is presented in Figure 2 to provide context to the soil moisture results presented. While there was plentiful rainfall during winter, spring was dry. The rainfall during December and January contributed to replenishing stored soil moisture, however there was little follow-on rainfall during February and March. Monthly rainfall (mm) 90 80 70 60 50 40 30 20 10 0 Jun 15 Jul 15 Aug 15 Sep 15 Oct 15 Nov 15 Dec 15 Jan 16 Feb 16 Mar 16 FIGURE 2 Monthy rainfall recorded at Rutherglen, June 2015–March 2016 RESEARCH AT WORK 67 East Rutherglen Carlyle Jun-15 50 Dec-15 Apr-16 Nitrate-N (kg/ha) Nitrate-N (kg/ha) Farmers inspiring farmers 40 30 20 10 0 Location:0–10 Springhurst/Lilliput 10–20 20–30 Soil depth (cm) 30–60 60–100 Dec-15 Apr-16 150 100 50 0 Location: Rutherglen 0–10 East 10–20 20–30 30–60 60–100 Soil depthCanola, (cm) stubble burnt 2014 crop and stubble practice: 2014 crop and stubble practice: Wheat, stubble burnt 2015 nitrogen applied: 9kg N/ha MAP, 37kg N/ha urea 2015 nitrogen applied: 8kg N/ha MAP, 101kg N/ha urea Timing of 2015 in-crop nitrogen application: Mid-June Timing of 2015 in-crop nitrogen application: Late July, early August 2015 stubble management (post-harvest): Stubble burnt 2015 stubble management (post-harvest): Stubble burnt Plains throughout most of the Soil moisture was Browns non-limiting 2015 cropping season at Springhurst/Lilliput, Victoria, Dec-15 Apr-16 until 80the season dried off duringJun-15 September, at which point60soil moisture started to withdraw down to 50cm. Rainfall 40 during November wet the profile somewhat, to a Norong depth of 30cm, however the soil Central had dried out in the top 20 10cm0 layer by sowing (Figure 3). Cornishtown The East Rutherglen site maintained high soil moisture levels through the season, until mid-September 2015 Jun-15 Dec-15 Apr-16 50 when the season turned dry (Figure 5). While the 40 10cm soil layer showed strong extraction of stored soil 30 moisture, only a small change is seen at 30cm, due to the 20 Dugays Bridge high subsoil clay content at this site. However, the large 10 decrease in plant available soil nitrogen (nitrate-nitrogen) 0 0–10 to December 10–20 20–30 30–60 60–100indicates Jun-15 Dec-15 Apr-16 from 70 June 2015 (post-harvest) Soil depth (cm) 60 plants were extracting nutrients (and therefore water) to 50 a depth 40 of at least 60cm. 30–60 Jun-15 60–100 Dec-15 Apr-16 Nitrate-N (kg/ha) 2 2 2 2 Nitrate-N (kg/ha) Nitrate-N (kg/ha) Soil moisture (mm) 6/0 il moisture6/0(mm) Soil moisture (mm)6/0 Soil moisture (mm)6/0 Soil moisture (mm) 5/ 5/ 5/ 5/ 20 2 20 20 01 Nitrate-N (kg/ha) Nitrate-N 15 15 15(kg/ha) 5 7/ 7/ 7/ 7/ 07 07 07 07 /2 /2 /2 /2 01 01 01 01 5 5 5 5 18 18 18 18 /0 /0 /0 /0 8/ 8/ 8/ 8/ 20 20 20 20 15 15 15 15 29 29 29 29 /0 /0 /0 /0 9/ 9/ 9/ 9/ 20 20 20 20 15 15 15 15 10 10 10 10 /1 / / / 1 11 11 1/ 1/ /2 /2 20 20 01 01 15 15 5 5 22 22 22 22 /1 /1 /1 /1 2/ 2/ 2/ 2/ 20 20 20 20 15 15 15 15 2/ 2/ 2/ 2/ 02 02 02 02 /2 /2 /2 /2 01 01 01 01 6 6 6 6 15 15 15 15 /0 /0 /0 /0 3/ 3 3/ 3/ /2 20 20 20 01 16 16 16 6 20 15 10cm 30cm 50cm 90cm 1030 South West Rutherglen 25 5 20 015 10–20 20–30 30–60 60–100 Jun-15 Dec-15 Apr-16 10 300–10 Soil depth (cm) 5 25 0 20 Springhurst/Lilliput 15 10 10cm 30cm 50cm 90cm 5 30 0 FIGURE levels at 20–30 Springhurst/Lilliput, Victoria 25 3 Soil moisture Springhurst/Lilliput 0–10 10–20 30–60 60–100 20 June 2015–March 2016 Soil depth (cm) 15 10 Jun-15 Dec-15 Apr-16 60 5 50 0 40 Dugays Bridge 30 20 10cm 30cm 50cm 90cm 1030 025 20 0–10 10–20 20–30 30–60 60–100 Carlyle 15 Soil depth (cm) 10 5 FIGURE 4 Plant-available (nitrate) soil nitrogen levels, at Jun-15 Dec-15 Apr-16 0 50 /Lilliput, Victoria June 2015–April 2016 Springhurst Dugays Bridge 40 30 30 20 25 20 10 15 0 10 5 0 40 30 10cm 0–10 30cm 10–20 20–30 30–60 Norong SoilCentral depth (cm) 10cm 30cm RESEARCH FOR THE RIVERINE PLAINS 2016 20 10 50cm 50cm 90cm 60–100 90cm Nitrate-N (kg/ha) 20–30 30 20 The June 2015 nitrate-nitrogen sampling showed a large 10 amount 0 of nitrogen (170kg N/ha) at the 0–10cm depth 0–10 20–30 30–60 before 101kg N/ha 10–20 was applied as urea during60–100 late July/ Norong depth (cm) early August (Figure 6). Soil This indicates plants potentially had access to 271kg N/ha during late winter. As it was quite Jun-15 Dec-15 Apr-16 35 at this time, it is likely East Rutherglen wet some of this nitrogen was lost as 30 25 gaseous-nitrogen due to denitrification (gaseous-nitrogen 20 30cm 90cm loss), while the rest was taken up10cm by the crop.50cm Conversely, 15 30 Wahgunyah 10 25 the lack of change in DSN (60–100cm) indicates that while 5 20 the 0 crop 15 may not have used this nitrogen, it is unlikely to 0–10 10–20 20–30 30–60 60–100 Apr-16 Jun-15 have10 leached deeperSoil due to the high clayDec-15 content at this 35 depth (cm) 5 site. 30 Summer mineralisation resulted in almost 40kg N/ha 0 25 East Rutherglen 20 to the following crop. available Nitrate-N (kg/ha) 10–20 26 26 26 26 /0 /0 /0 Nitrate-N Nitrate-N (kg/ha) Soil moisture (mm)(kg/ha) Soil moisture (mm) il moisture (mm) Soil moisture (mm) /05 Soil moisture (mm) 5/ 5/ 5/ /2 20 20 20 01 15 15 1 5 5 7/ 7/ 7/ 7/ 07 07 07 07 /2 /2 /2 /2 01 01 01 01 5 5 5 5 18 1 18 18 8 /0 /0 /0 /0 8/ 8/ 8/ 8/ 20 20 20 20 15 15 15 15 29 29 29 29 /0 / /0 /0 09 9/ 9/ 9/ /2 20 20 20 01 15 15 15 5 10 10 10 10 /1 /1 / / 1 1 1/ 1/ 1/ 1/ 20 20 20 20 15 15 15 15 22 22 22 22 /1 /1 /1 /1 2/ 2/ 2/ 2/ 20 20 20 20 15 15 15 15 2/ 2/ 2/ 2/ 02 02 02 02 /2 /2 /2 /2 01 01 01 01 6 6 6 6 15 15 15 15 /0 /0 /0 /0 3/ 3/ 3/ 3/ 20 20 20 20 16 16 16 16 0–10 Nitrate-N (kg/ha) 2015 crop and yield: Wheat, 5t/ha Nitrate-N (kg/ha) 2015 crop and yield: Wheat, 3.9t/ha The June352015 nitrogenSoil sampling showed a large bulge depth (cm) 30 of nitrate25(plant-available nitrogen) at 30–100cm depth, which is 20 likely due to accumulation of nitrogen over time, 15 (Figure 4). 10 This bulge largely disappeared by the post5 harvest sampling, with limited nitrogen remaining by the 0 0–10 10–20 20–30 60–100 pre-sowing sampling. While the crop 30–60 may have used Indigo depth (cm) some of this DSN, it is likelySoil at least some of the nitrogen in the 60–100cm layer was lost through leaching while Jun-15 Dec-15 Apr-16 25 Springhurst/Lilliput soil moisture levels were high throughout the season. 68 Jun-15 200 15 10 305 250 20 15 10 5 0 10cm 0–10 10–20 20–30 30cm 30–60 50cm 90cm 60–100 Soil depth (cm) Carlyle 10cm 30cm 50cm 90cm 10cm 30cm 50cm 90cm 30 25 FIGURE 5 Soil moisture levels at East Rutherglen, Victoria 20 June 15 2015–March 2016 East Rutherglen * The sharp 10 drop in soil moisture in late February is due to a damaged probe. 5 0 Jun-15 Dec-15 Apr-16 200 Carlyle 150 30 100 25 50 20 15 0 10 5 0 0–10 10–20 20–30 30–60 SoilNorong depth (cm) 60–100 30cm levels, 50cm at 90cm FIGURE 6 Plant-available soil10cm nitrogen East 40 Rutherglen, Victoria June 2015–April 2016 30 20 10 Soil moisture (mm) 10 0 16 /2 0 /2 0 26 /0 5 /0 3 15 22 2/ 02 /2 0 /2 0 /1 2 /1 1/ 20 10 /2 0 29 /0 9 /2 0 /0 8 18 /2 0 Soil moisture (mm) 20 10 /0 5/ 20 0 26 200 150 100 50 6 Soil moisture (mm) 30 0 25 20 15 10 5 0 0 01 26 / 05 /2 3/ 2 15 /0 02 /2 01 01 6 5 60–100 2/ 2/ 2 /1 90cm Nitrate-N (kg/ha) 50cm 30–60 22 10 /1 1/ 20 15 15 Soil depth (cm) 20 15 20–30 /0 9/ 29 20 15 18 /0 8/ 20 15 20 15 10 5 0 10–20 Nitrate-N (kg/ha) /2 0 /0 3 15 22 2/ 02 /2 0 /2 0 /1 2 /1 1/ 20 10 /2 0 29 /0 9 /2 0 /0 8 18 /2 0 0–10 35 30 25 20 15 10 5 40 0 30 16 16 15 15 15 15 15 15 30cm FIGURE 9 Soil moisture levels at Browns Plains, Victoria June 2015–March 2016 60–100 6 01 15 / 03 /2 6 01 2/ 02 /2 5 01 22 / 12 /2 15 10 /1 1/ 20 5 01 29 / 09 /2 5 01 18 / 08 /2 5 7/ 07 / 20 1 5 01 Dec-15 Apr-16 60 40 20 0 0–10 10–20 20–30 30–60 Soil depth (cm) 60–100 FIGURE 10 Plant-available soil nitrogen levels, at Browns Plains, Victoria June 2015–April 2016 50 40 30 20 25 20 0 15 10 5 0 10 5/ /0 26 Apr-16 Indigo Nitrate-N (kg/ha) Dec-15 Jun-15 80 20 6 15 / 03 / 20 1 6 2/ 02 / 20 1 5 20 1 12 / 20 1 22 / 29 10 /1 1/ 15 /0 9/ 20 15 20 18 7/ /0 07 8/ /2 01 5 15 5/ 5 Soil depth (cm) Soil moisture (mm) 30–60 Nitrate-N (kg/ha) 20–30 Jun-15 25 Dec-15 Apr-16 20 15 10 5 0 RESEARCH AT WORK 0–10 10–20 20–30 Soil depth (cm) 30–60 60–100 Nitrate-N (kg/ha) 10–20 Jun-15 /2 05 7/ 07 10 30 0 25 Nitrate-N (kg/ha) 0–10 35 30 25 20 Cornishtown 15 10 5 10cm 30cm 50cm 90cm 0 30 0–10 10–20 20–30 30–60 60–100 25 20 Soil depth (cm) 15 10 FIGURE 8 Plant-available soil nitrogen levels, at Wahgunyah, 5 Victoria0 June 2015–April 2016 26 / 7/ 07 Nitrate-N (kg/ha) Soil moisture (mm) 20 07 / 15 90cm Apr-16 Figure 10 shows nitrogen distributed to depth during June, with the highest concentration in the 10–20cm soil layer. It appears plants were extracting nutrients through the profile, based on the low levels of nitrateCarlyle nitrogen present during December, and may have almost depleted all nutrient reserves by harvest. Mineralisation Jun-15 Dec-15 Apr-16 during 50 the summer months has provided some nitratenitrogen prior to sowing, although this is primarily only in 40 Browns Plains 30 the 0–10cm layer, as would be expected. 7/ 20 3/ /0 02 2/ 50cm Nitrate-N (kg/ha) 2 15 16 15 15 15 15 15 15 /2 0 /0 5 26 16 6 /2 20 2/ 01 15 15 /1 10 29 22 30cm 20 Browns Plains 0 /0 /1 1/ 9/ /0 /0 18 10cm 10 26 20 20 20 8/ /2 07 7/ 15 15 5 01 15 5/ /0 26 50 40 30 40 20 30 10 0 20 Dec-15 25 South West Rutherglen 20 The 15 10cm soil moisture sensor at Browns Plains was 10 10cm 30cm remaining 50cm 90cm faulty,30 so is not shown in Figure 9. The data 5 shows25 0 plants were extracting moisture down to a depth 0–10 10–20 20–30 30–60 60–100 20 of at least 50cm and likely a bit deeper, while the 90cm 15 Soil depth (cm) layer 10 shows a heavy clay layer with minimal potential to 5 extract0water (Figure 9). /2 0 16 /2 0 15 /0 3 /2 0 16 15 /2 0 22 2/ 02 /1 2 /1 1/ 20 15 15 /2 0 10 /0 9 15 /2 0 29 /0 8 18 7/ 07 /2 0 15 15 /2 0 26 Soil moisture (mm) 30 The June 20 nitrate-nitrogen samples show an accumulation in the100–10cm soil layer, with little nitrogen at depth (Figure0 8). As plants can freely extract water down to at least 50cm, it is likely the crop extracted nutrients from that layer also, which is supported by the absence of detectable nitrate-nitrogen in the 30–60cm soil layer. The lack of change in nitrate-nitrogen levels at the 60–100cm Dugays Bridge layer indicates plants are not extracting from that layer, which is supported by Figure 6, indicating the deep soil Jun-15 Dec-15 extraction Apr-16 layer may be high in clay, Wahgunyah with little penetration 70 60 or nutrients by plant roots. of water Jun-15 26 /0 5 90cm Nitrate-N (kg/ha) Soil moisture (mm) 50cm soil moisture at which point the crop started to run the soil moisture levels down (Figure 7). Although a soil pit was not available at this site, it appears to be free draining and lighter textured down to at least 50cm, with plants accessing moisture down to 50cm with ease. The fact the 50cm layer had a large range between the upper and lower limits Norong (field capacity and permanent wilting point) indicates a lighter texture, compared with 10cm the previous site at90cm East 30cm 50cm 40 Rutherglen, which is known to have a high clay content. 6 3/ 20 1 /0 20 16 15 2/ 90cm 60–100 60–100 30 2015 stubble management (post-harvest): Stubble burnt 20 30cm 30 2015 stubble management (post-harvest): Stubble burnt Soil moisture (mm)Nitrate-N (kg/ha) 50cm 60 Apr-16 30–60 70 60 50 40 30 20 40 10 0 30 Timing of 2015 in-crop nitrogen application: June, September /0 5/ 10cm 20 3/ 2 2/ 2 15 /0 0 Location: Browns Plains FIGURE 7 Soil moisture levels at Wahgunyah, Victoria June 2015–March 2016 Wahgunyah Dec-15 20–30 90cm Soil depth (cm) 10 26 Timing of 2015 in-crop nitrogen application: Granular urea during June, liquid during August 01 6 60–100 01 6 60 90cm 10–20 50cm West Rutherglen 2015 nitrogen applied: South 87kg N/ha urea Nitrate-N Soil moisture (mm) (kg/ha) 50cm Apr-16 0–10 30cm 2015 nitrogen applied: 46kg N/ha urea, 27L N/ha Sulsa (liquid urea — 100L/ha of product applied) 20 16 20 3/ /0 15 Dec-15 10cm 2015 crop and yield: Wheat, 5t/ha /0 5 16 /0 3 /2 0 16 2/ 20 15 16 20 2/ 90cm Norong Central 2014 crop and stubble practice: Canola, stubble burnt in windrows 25 20 15 The 10 Wahgunyah site maintained high 5 the season until mid-September, through 0 50cm Apr-16 2015 crop and yield: Wheat, 4.25t/ha Soil moisture (mm) 90cm Dec-15 2014 crop and stubble practice: Canola, stubble burnt Carlyle 50cm Jun-15 35 30 25 20 15 40 10 5 30 0 20 (kg/ha) Soil moistureNitrate-N (mm) 16 /2 0 15 /0 3 /2 0 /2 0 2/ 02 22 90cm 16 50cm 15 15 30cm /1 2 15 10 29 18 26 /1 1/ 20 /2 0 15 /0 9 /2 0 15 /0 8 7/ 07 /2 0 15 /2 0 /0 5 /0 3 /2 0 16 10cm 30 25 20 15 10 5 0 Location: Wahgunyah 15 2/ 20 90cm 16 50cm Soil moisture (mm) East Rutherglen 2 22 1 29 18 7 26 Norong Central 35 30 25 20 15 10 5 0 69 10cm 0–10 10 10–20 20–30 60–100 16 /2 0 16 /0 3 2015 crop and yield: Wheat, cut for hay 5t/ha 70 0–10 10–20 20–30 30–60 Nitrate-N (kg/ha) 16 6 20 01 /0 02 3/ /2 20 15 22 2/ /1 2/ /1 1/ 20 15 15 15 20 29 10 /0 8/ /0 9/ 20 15 5 01 /2 07 18 35 30 25 20 15 10 5 0 0–10 20 16 /0 3/ /2 01 6 02 15 2/ /1 2/ 20 15 20 15 22 10 /1 1/ 20 15 9/ /0 26 7/ /0 5/ 20 15 Nitrate-N (kg/ha) Soil moisture (mm) Dec-15 6 01 /2 03 5 01 /2 90cm 60–100 6 30–60 20 1 Soil depth (cm) Apr-16 50cm 02 / 20–30 5 10–20 12 60–100 Wahgunyah Browns Plains 15 / FIGURE 14 Plant-available soil nitrogen levels, at Carlyle, Victoria June 2015–April 2016 2/ Apr-16 0–10 20 1 Dec-15 90cm 60–100 30cm 22 / Jun-15 RESEARCH FOR THE RIVERINE PLAINS 2016 South Rutherglen SoilWest depth (cm) ) 0–100 35 30 25 20 15 10 5 0 /0 Nitrate-N (kg/ha) 26 Apr-16 0 11 / Norong 0 10cm 10 / 10 40 40 30 30 20 20 10 10 0 5 20 50cm 30–60 Norong Jun-15 50 9/ 20 1 Victoria30June 2015–April 2016 0–10 FIGURE 13 Soil moisture levels at Carlyle, Victoria June Carlyle 2015–March 2016 /0 0–100 30 10cm 30cm 50cm 90cm 25 20 20 10 15 0 0–10 10–20 20–30 30–60 60–100 10 Norong Central 5 Soil depth (cm) 0 10cm 30cm 50cm 90cm FIGURE 4012 Plant-available soil nitrogen levels, at Cornishtown, 20–30 29 40 29 Apr-16 20 15 Dec-15 8/ Jun-15 5 Indigo 30cm 70 60 50 40 30 20 10 0 Soil depth (cm) 8/ 20 1 5 0 10–20 /0 50 0–10 /0 Apr-16 June 10cm 18 90cm 18 50cm /2 01 5 30cm 5 10cm 30 FIGURE 2511 Soil moisture levels at Cornishtown, Victoria 20 2015–March 2016 15 * Soil moisture sensor not working during early March 2016. Cornishtown 10 Carlyle 15 10 5 30 0 25 20 15 10 5 0 07 3/ 20 16 Dugays Bridge 7/ 60–100 07 /2 01 20–30 30–60 Soil depth (cm) 90cm 7/ 10–20 50cm Nitrate-N (kg/ha) Soil moisture (mm) 0–10 30cm 20 15 10cm The crop at the Carlyle site appears to have had limited access to soil moisture (Figure 13), with the 0–10cm Norong Central sensor being the only one to show clear change with rainfall or crop water extraction. This indicates the site Apr-16 is located on a heavy clay, and/orJun-15 has a Dec-15 high amount of 35 30 run-off — both of which would limit the amount of rainfall 25 Rutherglen is supported by the infiltrating to depth. ThisEast suggestion 20 15 nitrate-nitrogen values shown in Figure 14, which reveal 10 30cm and 50cm 90cm 5 the nitrogen is mostly stored in10cm the 0–10 10–30cm 30 0 25 layers, with0–10 only minimal to 60cm. 10–20 nitrogen 20–30 moving 30–60 down 60–100 20 As most of the nitrate-nitrogen is in the topsoil layers, Soil depth (cm) 15 10 this, with additional in-crop nitrogen, are likely being 5 utilised0 by the crop. However it is likely that significant denitrification occurred on this site during the wet winter, which may have been subject to prolonged waterlogging due to its inability toSouth drain water. The amount of nitrateWest Rutherglen nitrogen mineralised during summer was surprisingly Jun-15 have Dec-15 Apr-16 low, suggesting the soil sample may been taken 30 from an 25 area of bare ground, from which there was limited 20 matter (OM) to drive the mineralisation process. organic 5/ 100 30 50 25 200 15 10 5 0 2015 stubble management (post-harvest): Stubble burnt /0 16 /0 3 /2 0 16 /2 0 15 22 10 29 2/ 02 /2 0 15 15 /1 2 /1 1/ 20 15 /2 0 /0 9 15 /2 0 18 /0 8 15 /2 0 15 7/ 07 /0 5 Springhurst/Lilliput This correlates well with the nitrate-nitrogen values (Figure 12). While there was measurable nitrate-nitrogen 10cm 30cm 50cm 90cm to depth 30 during June, East although probably less than optimum Rutherglen 25 in the 0–10cm layer, the crop depleted the nutrient stores 20 15 to depth by harvest. However, the stores of nitrogen in 10 Jun-15 Dec-15 Apr-16 the topsoil were replenished with summer mineralisation 2005 0 Cornishtown of nitrogen. 150 Timing of 2015 in-crop nitrogen application: Late June, August 26 Nitrate-N Soil moisture (mm) (kg/ha) content with depth, based on the staged decrease in soil Soil depth (cm) 10 moisture at each depth from late September to the start of 0 November, with the November rain event only impacting on the 10cm layer. 2015 nitrogen applied: 74kg N/ha urea Nitrate-N(mm) (kg/ha) Soil moisture 5/ 20 15 Apr-16 /0 Dec-15 26 Jun-15 Timing35of 2015 in-crop nitrogenWahgunyah application: Late June 30 25 2015 stubble management (post-harvest): Stubble burnt 20 15 10cm 30cm 50cm 90cm 40 10 The soil moisture graph from the Cornishtown site (Figure 5 30 0 11) indicates a soil profile that gradually increases in clay 0–10 10–20 20–30 30–60 60–100 20 ) 2015 nitrogen applied: 58kg N/ha urea Nitrate-N (kg/ha) 15 2014 crop and stubble practice: Canola, stubble burnt in windrows Nitrate-N (kg/ha) /2 0 2/ 02 /2 0 15 15 /1 2 /1 1/ 20 22 29 18 Location: Carlyle Wahgunyah 2015 crop and yield: Wheat, 5t/ha 26 0–100 30–60 90cm 26 26 (kg/ha) Nitrate-N 26 /0 (mm)Soil moisture (mm)2(kg/ha) Soil moistureNitrate-N (mm) 6/ Soil moisture Soil moisture (mm) /05 Soil moisture (mm) /0 5/ 0 /2 5/ 5/ 5/ 20 0 20 20 20 15 15 15 15 15 7/ 7/ 7/ 7/ 7/ 07 07 07 07 07 /2 /2 /2 /2 /2 01 01 01 01 01 5 5 5 5 5 1 1 8/ 8/ 18 18 18 08 08 /0 /0 /0 /2 /2 8/ 8/ 8/ 01 01 20 20 20 5 5 15 15 15 29 29 29 2 2 9/ /0 /0 9/ /0 0 9 9 0 9/ 9/ /2 /2 9/ 20 20 01 01 20 15 15 5 5 15 1 1 10 10 10 0/ 0/ 11 11 /1 /1 /1 1/ 1/ 1/ /2 /2 20 20 20 01 01 15 15 15 5 5 22 22 22 22 22 /1 /1 /1 /1 /1 2 2 2/ 2/ 2/ /2 /2 20 20 20 01 01 15 15 15 5 5 2/ 2/ 2/ 2 2 02 02 /0 /0 02 2/ 2/ /2 /2 /2 20 20 01 01 01 16 16 6 6 6 15 15 15 15 15 / / /0 /0 /0 03 03 3/ 3/ 3/ /2 /2 20 20 20 01 01 16 16 16 6 6 90cm 50cm 2014 crop and stubble practice: Wheat, stubble burnt /2 0 3/ 20 Apr-16 10 /2 0 15 15 /0 9 /0 8 /2 0 15 15 7/ 07 /0 5 26 16 0–100 30cm Soil depth (cm) 0 /2 0 3/ 20 90cm Apr-16 Norong Location: Cornishtown Apr-16 Dec-15 Farmers inspiring farmers 16 0–100 Jun-15 70 60 50 40 30 40 20 30 10 0 20 /2 0 90cm Nitrate-N Soil moisture (mm) (kg/ha) Apr-16 200 150 100 50 0 0–10 0 60–100 0–10 10–20 16 /2 0 16 15 30 20 10 16 30 25 /0 5 26 Soil moisture (mm) 16 5/ 2 20 26 /0 3/ /0 15 16 26 /0 5/ 2 20 03 / 15 / Nitrate-N (kg/ha) Soil moisture (mm) 1 2 26 /0 5/ /0 15 Dec-15 70 60 50 40 30 4 20 3 10 0 2 20 3/ /2 2/ 02 20 2/ /1 20 /1 1/ 10 20 9/ /0 29 22 Jun-15 71 Apr-16 ha) 60 Apr-16 ha) RESEARCH AT WORK Dec-15 1 16 15 15 15 15 20 8/ /0 18 /2 7/ 07 20 South West Rutherglen Springhurst/Lilliput Jun-15 /2 /2 0 15 /0 3 /2 0 16 2/ 02 / Apr-16 FIGURE 18 Plant-available soil nitrogen levels, at Norong Central, Victoria June 2015–April 2016 5/ FIGURE 16 Plant-available soil nitrogen levels, at Indigo, Victoria June 2015–April 2016 01 5 35 30 25 20 15 10 5 0 15 60–100 Dec-15 35 South West Rutherglen 30 Jun-15 Dec-15 Apr-16 25 10cm 30cm 50cm 90cm 20 30 15 25 10 20 5 15 0 10 0–10 10–20 20–30 30–60 60–100 5 0–10 10–20 20–30 30–60 60–100 Soil depth (cm) 0 Soil depth (cm) /0 Soil depth (cm) 30–60 2 Norong Nitrate-N Soil moisture (mm)(kg/ha) 5 20–30 20 15 20 12 / 15 20 22 / 10 60–100 3 Norong Central *Soil moisture sensors not working in early March 2016. Nitrate-N (kg/ha) 10 10–20 6 /2 02 2/ 22 30–60 /1 1/ 9/ 29 /0 18 /0 8/ /2 07 7/ 20–30 Soil depth (cm) 01 6 /0 15 15 0–10 01 15 /1 2/ 20 15 20 /1 1/ 10 29 18 /0 /0 9/ 8/ 20 20 15 15 5 10–20 01 5 01 5 0 0–10 20 15 0 26 16 01 /2 10 4 FIGURE 17 Soil moisture levels at Norong Central, Victoria June 2015–March 2016 Apr-16 20 0 90cm 20 26 25 50cm Soil moisture (mm) 30 Apr-16 30cm Jun-15 Dec-15 16 15 22 2/ 02 /1 2 /2 0 15 15 /1 1/ 20 10 /2 0 15 29 /0 9 /2 0 15 18 /0 8 /2 0 15 7/ 07 15 40 3 2 2 1 1 20 3/ /2 02 2/ /1 2/ 20 01 6 15 15 20 22 10 /1 1/ 01 5 9/ 2 29 /0 01 5 18 /0 8/ 2 5 01 /2 07 7/ FIGURE 15 Soil moisture levels at Indigo, Victoria June 2015– March 2016 Indigo Jun-15 Soil moisture (mm) Soil moisture (mm) /2 0 /0 5 60–100 07 /0 15 Nitrate-N (kg/ha) 7/ 20 3/ /2 02 20–30 30–60 Soil depth (cm) 90cm Soil moisture (mm) 16 6 01 15 2/ /1 22 2/ 50cm 40 Dec-15 10cm 5/ 2 10–20 30cm Jun-15 50 /0 0–10 01 5 5/ 2 20 20 /1 1/ 10cm 25 40 20 20 15 0 10 5 0 26 /0 15 15 20 10 9/ /0 29 18 7/ /0 07 8/ /2 20 15 5 01 15 20 5/ /0 26 Nitrate-N (kg/ha) Soil moisture (mm) 60 20 60–100 3 2 2 1 1 Dugays Bridge 100 5/ 30–60 Apr-16 While the soil moisture results indicate that the plant 10cm 30cm 50cm 90cm 50 30 roots may not have gone much past the 30cm layer, due 25 0 to the wet conditions, the nitrate-nitrogen at depth was 0–10 10–20 20–30 30–60 60–100 20 15 decreased by harvest, by plants Soil depth (cm) or through losses to 10 depth (Figure 18). Moreover, although nitrogen supply 5 0 was replenished during summer, presumably at depth through mineralisation, the amount measured at depth is surprising given most mineralisation processes occur in the top 0–10cm layer of soil, with limited movement to depth. One possibility may be that the location for the April samplingCornishtown was a stock camp during the summer Norong Central months. /0 20–30 15 22 10 29 18 10–20 Dec-15 most of the winter, as shown by the 10cm depth layer having a higher soil moisture content than the deeper layers (Figure 17). This can only occur if the topsoil has East Rutherglen a greater clay content than the subsoil, and so can hold more water (which is unlikely), or if the soil is saturated, Jun-15 Dec-15 Apr-16 200 free-standing water. Saturation is likely, as the 30cm with 150 also held more water than it would otherwise. layer Soil moisture (mm) 16 /0 3 /2 0 16 /2 0 2/ 02 /2 0 15 15 /1 2 /1 1/ 20 15 /0 9 /2 0 15 /0 8 /2 0 15 7/ 07 /2 0 15 /0 5 26 Nitrate-N (kg/ha) Soil moisture (mm) 0–10 15 10 nitrate-nitrogen Soilvalues depth (cm) The soil shown in Figure 16 5 support0 this observation. The even distribution of nitrogen through the profile during June indicates easy movement of nutrients down the profile, while the lack of measurable nitrogen post-harvest indicates the crop used this nitrogen within season, with leaching of nutrients below the measured depth also possible. Brownsnitrogen Plains Movement of mineralised over summer was also measured, with the 10–20cm depth recording as much IndigoJun-15 Dec-15 Apr-16 nitrate-nitrogen as is in the surface 0–10cm layer. 80 Nitrate-N (kg/ha) /0 15 The Indigo site looks to be quite free draining down to at least 30cm, as shown by the 10cm and 30cm depths in Figure 15, showing similar soil moisture storage. The range in soil moisture storage Carlyle(difference from wet to dry) in the 10cm and 30cm layers, and the rapidity with which the profile wet and drained, indicates thisDec-15 soil isApr-16 likely to Jun-15 have 50 a high gravel content.Cornishtown While infiltration decreased 40 down to 50cm, there were still some plant roots at depth, 30 as seen by water extraction by the 30cm crop during early 10cm 50cm 90cm 20 30 October 2015 after the 10cm and 30cm layers were 10 25 20 depleted of soil moisture. 0 a) 20 16 90cm 3/ 16 20 2/ 0 2015 stubble management (post-harvest): Stubble burnt /2 0 16 /2 0 /0 3 15 2/ 20 16 Soil depth (cm) July Timing10 of 2015 in-crop nitrogen application: 26 0 20 2015 nitrogen applied:10–20 10kg N/ha MAP, 46kg N/ha urea 0–10 20–30 30–60 60–100 Nitrate-N (kg/ha) 90cm Jun-15 35 30 Location: Norong Central 25 20 2014 15 crop and stubble practice: Wheat, stubble burnt Springhurst/Lilliput 10 2015 5 crop and yield: Wheat, 3t/ha 0 10cm 30cm 50cm 90cm 2015 nitrogen MAP, 92kg N/ha 60–100 urea 0–10 applied: 10–208kg N/ha 20–30 30–60 30 Soil depth (cm) Timing 25 of 2015 in-crop nitrogen application: Late July, late August 20 2015 stubble management (post-harvest): Stubble burnt 15 10 5 0 The Norong Central site appeared to be waterlogged 26 Apr-16 Nitrate-N (kg/ha) Dec-15 2015 crop 5 and yield: Feed wheat, 3.5t/ha 30 50cm 60–100 /0 3 /2 0 2/ 02 /2 0 15 15 /1 2 /1 1/ 20 Jun-15 30 Wahgunyah 25 Location: Indigo 20 15 and stubble practice: Wheat, 10cm 30cmburnt 50cm 2014 crop stubble 40 10 Nitrate-N (kg/ha) Soil moisture (mm) 90cm 30–60 Wahgunyah South West Rutherglen 50cm 20–30 Soil depth (cm) 22 29 10 /2 0 15 15 /0 9 /0 8 /2 0 15 /2 0 7/ 07 /2 0 /0 5 30–60 Soil depth (cm) 0 26 20–30 20 15 10–20 18 Soil moi 0–10 10 15 16 /2 0 16 /0 3 15 2/ 20 20 0 35 30 Nitrate-N ( 25 20 15 10 5 0 Farmers inspiring farmers 100 0–10 10–20 20–30 30–60 60–100 Soil depth (cm) East Rutherglen 90cm 16 2014 crop and stubble practice: Lucerne, two-way disc before cropping 16 /2 0 /0 3 Dec-15 Apr-16 Jun-15 10cm 30cm Dec-15 50cm 90cm 40 30 /2 01 0 Apr-16 re (mm) 35 3030 2525 2020 re (mm) 20 20 15 15 N (kg/ha) isture (mm) 30 30 07 10 7/ 07 /2 Soil moisture (mm) 20 20 15 03 / 15 / 30 Wahgunyah 10cm 30cm Dec-15 50cm Apr-16 90cm Jun-15 RESEARCH FOR THE RIVERINE PLAINS 2016 25 25 N (kg/ha) 72 01 7/ 26 6 6 02 / 2/ 22 / 10 / 29 / 18 / 7/ 26 FIGURE 22 Plant-available soil nitrogen levels, at Dugays Bridge, Victoria June 2015–April 2016 40 26 /0 5/ 90cm 60–100 20 1 5 12 / 20 1 5 11 / 20 1 5 50cm 30–60 Soil depth (cm) 20 1 5 20 1 20–30 09 / 07 / 20 5/ /0 10–20 30cm Apr-16 20 1 10cm Dec-15 South West Rutherglen 100 07 /2 01 5 Soil moisture (mm) 5/ 2 3/ 15 /0 30 25 20 15 10 5 0 /0 90cm 20 16 6 01 2/ Jun-15 0 0–10 Cornishtown South West Rutherglen 90cm 01 Soil moisture (mm) 15 20 5/ 7/ /0 15 /0 20 3/ /2 02 2/ 02 /2 20 15 2/ 20 15 /1 1/ 10 29 22 /1 5 /0 9/ 2 01 5 01 8/ 2 50cm Norong Central 70 60 5040 40 30 30 2020 10 10 0 15 Soil depth (cm) Apr-16 26 16 6 01 15 20 2/ /1 22 10 /1 1/ 20 15 15 20 9/ 29 /0 18 /0 8/ /2 20 15 5 01 15 07 7/ 5 7/ 07 /2 01 5/ 2 /0 26 Nitrate-N (kg/ha) 16 20 3/ /0 15 30cm FIGURE 21 Soil moisture levels at Dugays Bridge, Victoria Dugays Bridge June 2015–March 2016 Soil moisture (mm) Apr-16 6 2/ 02 /2 01 5 12 / 20 1 15 20 10 /1 1/ 22 / 5 29 / 09 / 20 1 5 18 / 08 / 20 1 5 01 7/ 07 /2 5 20 1 26 / 05 / 3/ 2 01 6 100 Dec-15 Soil moisture (mm) 90cm Jun-15 35 Wahgunyah 30 Norong Central 25 20 10cm 30cm 50cm 90cm 1540 10 Jun-15 Dec-15 Apr-16 35530 0 30 20 0–10 10–20 20–30 30–60 60–100 25 Soil depth (cm) 20 10 15 0 FIGURE 20 Plant-available soil nitrogen levels, at Norong, 10 Victoria 5 June 2015–April 2016 0 0–10 10–20 20–30 30–60 60–100 Nitrate-N (kg/ha) Nitrate-N (kg/ha) Apr-16 /0 6 01 /2 03 FIGURE 19 Soil moisture levels at Norong, Victoria June 2015–March 2016 Norong 10cm 30 25 20 15 10 5 0 08 / 2/ 15 / 02 12 /2 /2 01 01 6 5 15 20 10 22 / 9/ 29 /0 /1 1/ 20 15 15 20 8/ 20 /0 26 7/ 0 7/ 5/ /0 15 15 0 30 25 20 15 10 5 0 Dugays Bridge 18 10 5 60–100 Soil moisture (mm) 15 30–60 Soil depth (cm) 01 20–30 90cm 5 10–20 50cm 5 0 The nitrate-nitrogen results (Figure 22) show the crop accessed almost all available nitrogen reserves by harvest, with little nitrogen remaining in the profile at the end of the season. The poor mineralisation of nitratenitrogen during summer indicates most of the ‘potentially mineralisable nitrogen’ fraction in the total soil nitrogen bucket was accessed. This means the 2016 crop would require larger nitrogen inputs to meet its yield potential. 20 20 3/ /0 02 2/ 22 16 6 01 /2 20 /1 2/ /1 1/ 20 15 15 15 20 29 10 /0 9/ 20 8/ /0 18 0–10 20 30cm The Dugays Bridge soil moisture probe indicates this site is likely to be free draining, which would be expected as it is close to the Murray River (Figure 21). As such, it is unknown if there is any lateral water movement from the Springhurst/Lilliput river at the 50cm and 90cm deep soil layers. However, 10cm 30cm 50cm drawing 90cm when the 10cm and 30cm sensors started 30 moisture 25 during September, the 50cm sensor showed slight 20 drawdown, as did the 90cm sensor. This indicates 15 roots were accessing water at depth. 10 20 1 30 0 20 20 3/ 15 5 01 /2 07 10cm 40 10 16 100 20 18 90cm Norong 30 Soil moisture (mm) Apr-16 Nitrate-N (kg/ha) 26 7/ /0 3/ 5/ 20 20 16 15 Soil moisture (mm) The 50 soil moisture profile at Norong showed a high capacity to store and release moisture from the 10cm 0 Carlyle 0–10 20–30 30–60 60–100 depth, due to a 10–20 light-textured topsoil (Figure 19). Soil depth (cm) 10cm texture 30cm 50cm 90cm However, there was likely a large change into 30 the subsoil, with a large increase in clay content. This 25 would20 explain why the moisture release range of the 15 30cm 10 and 50cm soil layers is similar, and relatively small 5 (small 0difference between wet and dry moisture levels). The decline in soil moisture in the 30cm and 50cm depth during mid-October indicates plants were accessing to Cornishtown this depth, with the nitrate-nitrogen values supporting the soil moisture results (Figure 20). While some nitrogen Jun-15 Dec-15 Apr-16 may50be lost to depth, it is likely plants used most of the 40 nitrogen. 5/ 22 29 18 15 2015 stubble management (post-harvest): Stubble burnt 100 90cm 2015 nitrogen applied: 18kg N/ha DAP Timing of 2015 in-crop nitrogen application: None applied 2015150 stubble management (post-harvest): Stubble burnt 100 2015 crop and yield: Wheat, 2.5t/ha /0 /2 0 2/ 02 /2 0 /1 2 /1 1/ 20 /2 0 10 /0 9 /2 0 /0 8 /2 0 7/ 07 6/ 05 Jun-15 26 15 15 15 15 15 Location: Dugays Bridge 200of 2015 in-crop nitrogen application: June, August Timing Nitrate-N (kg/ha)2 3/ 20 50cm 2015 nitrogen applied: 9kg N/ha MAP, 74kg N/ha Urea /2 0 16 15 2015 crop and yield: Wheat, 4t/ha Apr-16 30cm 30 25 20 Location: Norong 15 10 2014 crop practice: Wheat, stubble burnt 5 and stubbleEast Rutherglen 0 Soil moisture (mm) 10cm Soil moisture (mm) 90cm Apr-16 40 20 10 0–10 10–20 20–30 30–60 60–100 / 15 2/ 22 16 /2 0 /0 3 15 2/ 02 /2 0 16 15 /2 0 /1 2 22 29 10 /1 1/ 20 15 15 /2 0 15 /0 9 /2 0 18 /0 8 /2 0 15 15 7/ 07 16 /2 0 16 15 /0 3 22 2/ 02 /2 0 /2 0 /1 2 10 /1 1/ 20 15 15 15 /2 0 15 29 /0 9 /2 0 15 18 /0 8 /2 0 15 7/ 07 16 6 20 01 15 2/ /0 02 3/ /2 20 2/ /1 22 10 /1 1/ 20 15 15 15 9/ 20 15 /0 29 18 /0 8/ /2 20 01 5 15 07 7/ Example of the value of incremental soil sampling Rutherglen, 2015 20–30 12.7 30–60 18.9 200 100 25 20 50 15 0 10 6 20 1 6 03 / 15 / 2/ 02 / 20 1 12 / 22 / 20 1 5 5 20 1 5 11 / 20 1 09 / 10 / 18 168.0 29 / /0 8/ 20 15 Nitrate-nitrogen Average soil nitrate-nitrogen (kg/ha) levels across incremental depths 67.2 150 60–100 30 0 Soil moisture (mm) /0 26 0–10 Soil depth (cm) 10–20 Nitrate-N (kg/ha) Dec-15 Depth (cm) isture (mm) Nitrate-N (kg/ha) Jun-15 20 15 10 1 TABLE 5 from East 0 01 5 01 6 3/ 2 15 /0 2/ 2 01 6 at South-west 10 5 30cm 50cmof nitrate90cm The Springhurst/Lilliput site has 10cm a larger amount 30 0 0–10 10–20 20–30 30–60 60–100 25 nitrogen at depth than the east Rutherglen (Table 2). /2 60–100 Carlyle 50 /2 0 /0 90cm 15 50cm 2/ 0 01 5 2/ 2 01 5 22 /1 10 /1 1/ 2 01 5 9/ 2 29 /0 01 5 8/ 2 01 5 18 /0 7/ 2 7/ 0 01 5 5/ 2 26 /0 Apr-16 Browns Plains 10–20 20–30 30–60 25 0–10 20 Soil depth (cm) 15 10 24 Plant-available soil nitrogen levels, FIGURE 5 Rutherglen, Victoria June 2015–April 2016 0 0 Based on the 0–100cm depth average of 56kg N/ha at East Rutherglen in Table 1, a grower may decide to apply more nitrogen to the crop soon after sowing. However, based on the 0–30 and 30–100cm averages, which show Wahgunyah plentiful nitrogen in the surface soil available for plant uptake, the grower may decide to apply less nitrogen Dec-15 Apr-16 early35in the season, reserving someJun-15 for topdressing later 30 the season if the conditions (and soil moisture during 25 availability) allow, as thereCornishtown is little nitrogen available at 20 15 depth for plants to access through spring. 26 15 Dec-15 30cm 40 10 07 16 3/ /0 02 /2 20 01 6 15 22 2/ /1 2/ 20 15 20 /1 1/ 15 29 10 /0 9/ 20 18 /0 8/ /2 07 7/ 20 15 5 01 15 Jun-15 Soil moisture (mm) Nitrate-N (kg/ha) FIGURE 23 Soil moisture levels at South-west Rutherglen, South 2016 West Rutherglen Victoria June 2015–March 30 25 20 15 10 5 030 Soil moisture (mm) 26 60–100 Soil depth (cm) 90cm Two 0examples from the June 2015 dataset are shown 30 0–10 10–20 20–30 30–60 60–100 in Table 1 and Table 2 to demonstrate the value of 20 incremental sampling. Soil depth (cm) 10 7/ 30–60 90cm 50cm 10 /0 5 16 /2 0 15 50cm 30cm Splitting cores into more than two increments would Jun-15 Dec-15 Apr-16 70 become cost-prohibitive, however even the simple 60 change can provide useful information 50 to two incrementsWahgunyah on 40 nutrient availability, resulting in more informed 30 decision-making and greater environmental stewardship. 10cm 30cm 50cm 90cm 20 Nitrate-N (kg/ha) 20–30 /0 3 /2 0 2/ 02 30cm 90cm Feedback to date from this project is that some growers 0 are already changing the way they carry out their sampling, with the soil core being broken up into at least two increments (0–30cm and 30–60cm) to better Dugays Bridge understand where the nitrogen is stored. Soil moisture (mm) 10–20 16 15 /2 0 22 /1 2 15 15 10 /1 1/ 20 /2 0 29 /0 9 15 /2 0 /0 8 15 0–10 20 5/ /0 18 7/ 07 10cm 15 10 5 0 26 /2 0 15 /2 0 26 /0 5 Nitrate-N (kg/ha) Soil moisture (mm) 20 15 1030 525 020 50cm incremental depth sampling 10cm 26 Soil moisture (mm) 0 The increase in nitrate-nitrogen down to 60cm indicates nitrogen can be easily leached in this soil (Figure 24). While plants are likely to take up most of this nitrogen, as roots accessed this depth, there is potential for nitrogen Norong Central to be lost deeper in the soil profile. This is seen from the April sampling, where the nitrate-nitrogen values Jun-15 Dec-15 Apr-16 35 increased at the 60–100cm soil depth. This demonstrates 30 the ability of this soil to move nutrients to depth. 25 South West Rutherglen 30cm 40 The incremental depth sampling was carried out to help 30 growers appreciate the averaged deep nitrogen sample 20 has limitations. Nitrate-N (kg/ha) Norong Central The soil moisture profile of the South-west (SW) 10cmhad 30cm 50cm capacity 90cm Rutherglen site indicates this soil plentiful 40 to store and release water for plant growth, with plants 30 accessing moisture down to at least 50cm, if not deeper 20 (Figure 10 23). Soil moisture (mm) 2015 stubble management (post-harvest): Stubble burnt 20 Timing of 2015 in-crop nitrogen application: July 10cm practice for sampling DSN is to take a core soil sample down to 60cm or 100cm, homogenise the sample and take a subsample from that for laboratory analysis. This effectively provides an average nitrogen sample for the whole core. While this provides an indicator of stored nitrogen across the whole season, it doesn’t provide any information on when the plants will Norong be able to access this nitrogen. /2 0 16 /0 3 15 2/ 02 /1 2 22 10 29 /0 9 /0 8 18 7/ 07 /2 0 16 /2 0 15 /2 0 15 /1 1/ 20 /2 0 15 15 /2 0 /2 0 15 15 /2 0 26 /0 5 2015 nitrogen applied: 10kg N/ha MAP, 46kg N/ha urea 30 25 20 15 Importance of 10 5 The accepted 0 /0 5 90cm 5/ 50cm 20 30cm 5/ Soil moisture (mm) 10cm 30 25 20 15 Location: South-west Rutherglen 10 5 2014 crop and stubble practice: Canola, stubble burnt in windrows 0 2015 crop and yield: Wheat, 4.9t/ha / / 10 / 29 / 18 7/ / 26 / 15 2/ / / 10 / 29 / 18 7/ / 26 22 Carlyle Dugays Bridge Average 0–30cm = 82kg/ha East Rutherglen Average 0–100cm = 56kg/ha Average 30– Jun-15 Indigo 100cm = 17kg/ha Dec-15 10cm 50cm Apr-16 15.1 30cm 90cm RESEARCH AT WORK 73 Farmers inspiring farmers TABLE 2 Examples of the value of incremental soil sampling from Springhurst/Lilliput, 2015 Depth (cm) Nitrate-nitrogen (kg/ha) 0–10 21.0 10–20 15.4 20–30 13.4 30–60 42.0 60–100 49.3 Average soil nitrate-nitrogen levels across incremental depths Average 0–30cm = 17kg/ha Average 0–100cm = 28kg/ha Average 30–100cm = 46kg/ha If a grower used the results from the 0–100cm sample (28kg N/ha), a decision could be made to apply a lot of nitrogen early during the season due to a perceived nitrogen deficit through the profile. However, if the core was split into the 0–30cm and 30–100cm layers, the grower would identify the shortfall of nitrogen in the top layers, but also see there is some nitrogen sitting at depth, which could contribute to finishing the crop off in a favourable season without additional nitrogen being applied. Total nitrogen vs available nitrogen The results presented to date mostly focus on the available soil nitrogen (measured as nitrate-nitrogen), which is the form of nitrogen crops access during the growing season. Another form of plant-available nitrogen is ‘mineral nitrogen’, which includes both nitrate (NO3) and ammonium (NH4) forms of nitrogen, which is a more complete measure of available nitrogen. However, as some of the ammonium results received during this project were questionable, the ammonium results were omitted. A key part of the nitrogen story for crop production is the cycling of nitrogen through organic (plant matter, decaying roots, microbial biomass) and inorganic (nitrate, ammonium) forms. To this end, we measured the total soil nitrogen in addition to the available/nitratenitrogen to understand the size of the complete soil nitrogen ‘bucket’, which is already in the soil, additional to any fertiliser applied. The real challenge lies in estimating the nitrogen mineralisation rate — the rate at which the organic (plant unavailable) nitrogen is transformed into plant-available forms. The mineralisation process depends heavily upon environmental factors including temperature and moisture, and also varies according to the type and amount of plant residue present on the ground. A comparison of total nitrogen vs plant-available nitrogen is presented in Figure 25. Figure 25 illustrates an accumulation of organic nitrogen (represented as totalnitrogen minus nitrate-nitrogen) in the 0–10cm layer, which is expected as this is where most of the nutrientcycling microbes are located. To provide context as to the size of this organic nitrogen ‘bucket’, nitrate-nitrogen is plotted alongside, and barely registers on the scale of this graph. Nitrate-nitrogen (%) 0.20 Total nitrogen (%) 0.18 0.16 Nitrogen (%) 0.14 0.12 0.10 0.08 0.06 0.04 0 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0–10 10–20 20–30 30–60 60–100 0.02 Norong Central Dugays Bridge South west Wahgunyah Rutherglen Carlyle East Rutherglen Browns Plains FIGURE 25 A comparison of total nitrogen vs plant-available (nitrate) nitrogen 74 RESEARCH FOR THE RIVERINE PLAINS 2016 Indigo Cornishtown Springhurst Lilliput Norong Observations and comments Although all of the sites for this project lie within the Rutherglen region, there is a range of soil types. However, the duplex, texture-contrast soil type dominates, with a sharp increase in clay content in the subsoil. This means water is strongly held in this zone and is only extracted for plant use after the topsoil layers are depleted of moisture. The sites vary in both the amount of nitrate-nitrogen stored in the soil, and the depth at which it is stored. As the traditional DSN measure doesn’t account for the distribution of nitrogen, it is worth considering splitting DSN samples into two increments — a 0–30cm and a 30+cm depth sample. This will provide more accurate information on the availability of nitrogen as the season progresses, supporting better and more timely fertiliser application decisions. The nitrate-nitrogen component is only a small proportion of the total soil nitrogen, with the large pool of soil organic nitrogen being responsible for the ongoing cycling and mineralisation throughout the season and during summer. While the soil moisture results presented here show a dry soil profile going into the 2016 season, as this report is being written the ground outside has turned to mud, thanks to the fantastic rain received during early May. With between 60–80mm rainfall across the region, it certainly will go a long way towards refilling the soil profile! Acknowledgements This project was carried out in partnership with the North East Catchment Management Authority (NECMA) with funding from the Australian Government’s National Landcare Programme and Sustainable Agriculture Victoria: Fast-Tracking Innovation, an initiative made possible with the support of the Foundation for Rural and Regional Renewal (FRRR) together with the William Buckland Foundation. Many thanks to the growers who participated in this project. Contact Dr Cassandra Schefe, Riverine Plains Inc T:(03) 5744 1713 E:[email protected] RESEARCH AT WORK 75
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