Solubility of Luciferin Free Acid
O
HO
N
N
S
S
OH
D-Luciferin Free Acid
C11H8N2O3S2
280.32 g/mol
Solvent System
Soluble/Insoluble
Comments
DMSO
DMSO/water
Methanol
Methanol/water
Methanol/water
Ethanol
Ethanol/water
Acetone
Acetone/water
DMF
DMF/water
THF
THF/water
ACN
ACN/water
1,4-Dioxane
1,4-Dioxane/water
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Soluble
Insoluble
Soluble
Soluble
Soluble
Solubility = 10 mg in 50 µL DMSO
Solubility = 10 mg in 0.50 mL DMSO/water (1:1, v/v)
Solubility = 10 mg in 2.0 mL Methanol
Solubility = 10 mg in 15.0 mL Methanol/water (1:1, v/v)
Solubility = 10 mg in 4.0 mL Methanol and 0.5 mL water
Solubility = 10 mg in 5.0 mL Ethanol
Solubility = 10 mg in 2.0 mL Ethanol and 0.5 mL water
Solubility = 10 mg in 4.0 mL Acetone
Solubility = 10 mg in 2.0 mL Acetone and 0.5 mL water
Solubility = 10 mg in 0.1 mL DMF
Solubility = 10 mg in 0.1 mL DMF and 0.1 mL water
Solubility = 10 mg in 2.0 mL THF
Solubility = 10 mg in 1.0 mL THF and 50 µL water
10 mg is not soluble in 10.0 mL ACN
Solubility = 10 mg in 5.0 mL ACN and 0.5 mL water
Solubility = 10 mg in 8.0 mL 1,4-Dioxane
Solubility = 10 mg in 1.0 mL 1,4-Dioxane and 0.25 mL water
To solubilize D-Luciferin free acid in water
1) Sparge distilled water with an inert gas (nitrogen or argon) for 15-30 minutes at ambient
temperature. Add D-Luciferin free acid (10 mg) to 0.50-1.0 mL of oxygen-free water (sparge
water).
2) Add either 2.95 mg of sodium bicarbonate or 3.50 mg of potassium bicarbonate.
3) Allow the mixture to stir for 30 minutes under an inert atmosphere. The mixture should be a
yellow solution. Caution: If too much sodium or potassium bicarbonate is added to D-Luciferin
free acid, the mixture will become oxidized and the chiral purity may be affected.
Regis Technologies Inc.
8210 Austin Avenue Morton Grove, IL 60053
www.regislucifern.com