light sources
micropipette fabric
SUTTER INSTRUMENT
micromanipulatio
optical instruments
2 0 0 6
ropipette fabrication
optical instruments
croinjection
micropipett
cromanipulation
filter wheels
2006
microinjection
microscope accessories
2006_catalog.indd 2-3
PRICES
SERVICE & SUPPORT
Prices included herein are for items
sold in the USA or Canada, F.O.B.
Novato, California (exclusive of transportation, insurance, and applicable
taxes), and are subject to change
without notice. International prices
are 5% higher. Please contact Sutter
Instrument or your local representative for an overseas price quotation.
We hope that our instruments and
products continually meet your
needs. However, should a problem
arise, please contact our technical
support staff to discuss the problem.
If the instrument requires factory service, we will furnish shipping instructions. Items under warranty will be
repaired free of any costs, for parts
or service. Both delivery and return
shipping costs are the responsibility
of the owner.
SHIPPING
Shipping charges are prepaid and
added to our invoice. Unless otherwise specified when placing your
order, we will use our best judgement
in selecting a reliable and economical
shipper of our choice.
RETURNS
Items ordered in error may be returned within 30 days of receipt and
are subject to a 15% restocking fee.
You are urged to retain the original
shipping containers should there be
a need to return the item. Please
contact Sutter Instrument for a return
authorization number.
QUALITY CONTROL
WARRANTY INFORMATION
Sutter Instrument Company provides
a limited warranty for one year
from shipping date, on all Sutter
made products and labor (except
consumables and Uniblitz® shutter).
To be covered under warranty, the
instrument must have been operated
in accordance with the instructions
outlined in the instruction manual
and in a manner that would be
expected in the normal use of the
product. Extended warranties may be
purchased for an additional charge.
Please contact Sutter for a quotation.
Abuse, misuse, or unauthorized
repairs will void any warranty.
Sutter Instrument takes great pride PAYMENT METHODS
in meeting the highest possible stanPayments may be made in U.S. Dollars
dards of quality and reliability. Each in one of the following methods:
instrument undergoes a rigorous
electronic and/or mechanical testing
Major Credit Card: Master Card
protocol during the production proand VISA
cess. In the case of our micropipette
Bank Draft drawn on a
pullers, every instrument is tested to
U.S bank
assure its ability to consistently fabricate micropipettes with ultra-fine
International Money Order
tips. A series of pipettes are pulled
with each instrument and examined
Bank Wire Transfer
with our scanning electron microscope. No other manufacturer offers
Irrevocable Letter of Credit
this level of quality control.
(a processing fee will apply)
10/3/05 5:06:39 PM
INDEX
MICROPIPETTE FABRICATION
MICROMANIPULATION
Introduction
Introduction
4
PULLERS
P-2000 Laser-Based
Micropipette Puller
6
10
P-30 Vertical Puller
14
Filaments/Accessories
16
BEVELER
18
PRESSURE INJECTOR
Manual Microinjector
22
24
GLASS
Glass Capillary Tubing
34
MP-285 Robotic
Micromanipulator
40
MP-225 Motorized
Micromanipulator
46
MP-85 Huxley-Wall Style
Micromanipulator
50
MM-1 & MM-3
Micropositioners
26
80
Lambda DG-4/DG-5
MPC-200/MPC-385/MPC-325
Multimanipulator System
MM-33 Coarse
Micromanipulator
Introduction
MICROINJECTION
WAVELENGTH SWITCHER
54
Introduction
120
XenoWorks Micromanipulator 126
82
FILTER WHEEL SYSTEMS
Lambda 10-3
88
Lambda 10-2
94
Lambda 10-B
100
SHUTTERING
Digital Microinjector
130
Analog Injector
134
PMM-150FU
136
CUSTOM PRODUCTS
TM
SmartShutter
104
Lambda SC
108
OEM Solutions
138
LIGHT SOURCE
58
MOUNTING SYSTEMS
BOOKS
Advanced Techniques in
Micropipette Fabrication
32
MANIPULATORS
P-97 Flaming/Brown
Micropipette Puller
BV-10 Microelectrode
Beveler
OPTICAL INSTRUMENTS
MT-2000 Motorized
X-Y Translator
62
MT-1000 X-Y
Translation System
64
MT-70 Stands
68
MT-75 Gantry
72
MD-Series Platforms
76
Outdated
AutoMate Scientific
Lambda
LS prices. Please see the
DEALERS
store Light
for current
Xenon
Source prices. 110
International Dealer
MICROSCOPE ADAPTERS
Directory
Adapter list
web
140
114
800.998.MATE | www.autom8.com | 812 Page St, Berkeley, CA 94710 USA
tel 510.845.6283 | fax 510.280.3795 | e-mail [email protected]
2006_catalog.indd 2-3
10/3/05 5:06:39 PM
P-2000
LASER-BASED MICROPIPETTE PULLER
MICROMANIPULATION
new photo
Precise movement. Just what you
require in a micromanipulator. Sutter
Instrument, the same company that
refined the movements necessary to
repeatedly pull micropipettes with
sub-micron tips, has also harnessed
the technologies necessary to easily
move those same tips with sub- micron accuracy.
Micromanipulator design at Sutter
Instrument began in 1985 with the
MP-85, a refined and extended Huxley-style micromanipulator. Continuing from that time Sutter Instrument
has shown a desire to provide manipulator mechanisms with smooth,
ergonometric micro-movements that
are also adaptable to many different
experimental designs and platforms.
2006_catalog.indd 32-33
This design process includes two motorized manipulators, the industry
standard MP-285 and its new sibling, the MP-225. Both couple the
smooth mechanical micromotion and
microprocessor-based, stepper-motor
drive mechanisms users have come to
expect in Sutter products.
As time has shown, much of what
makes a scientific apparatus popular
is how easy it can be incorporated
into existing equipment. In the case
of micromanipulators, this process has
involved designing manipulator platforms that bolt directly to the frames
of the most popular microscopes. Alternatively, we also offer rock-steady,
free-standing platforms that support
our manipulators by clamping to the
table beside the microscope. Our
original stands, the MT-70 and MT75, originally designed to support
the fifteen- pound Huxley with zero
sag, also double as free-standing
platforms for the MP-225 and MP285. The motorized manipulators
are also supported by a number of
microscope-specific stands that bolt
directly to the bodies of the most
popular Leica, Nikon, Olympus, and
Zeiss inverted microscopes. These
platforms lend themselves to the
absolutely drift-free recording configuration required when using the
MP-225 and MP-285 for positioning
patch-electrodes onto attached cells
in culture.
The newly introduced MT-2000 uses
a motorized X-Y translator. In this
design, stepper-motor driven lead
screws provide smooth movement of
the microscope.
Rounding out our line of manipulators are the MM-33 micromanipulator and the MM-1 and MM-3
micropositioners. The MM-33 is an
economical micromanipulator of
sufficient resolution and control for
impaling xenopus oocytes and other
medium-fine manipulation tasks. The
MM-1 and the MM-3 are suitable for
positioning perfusion tubes, moving
a preparation, or for positioning reference electrodes in a bath.
A task specific platform for manipulators is the MT-1000 translation
system. When coupled with MP285s or MP-225s, the system forms
a slice-patch workstation. The MT1000 was born out of the technique
now common in slice recording that
moves the optical pathway while
keeping the slice and recording
electrodes fixed. The translation
system allows the user to move to
multiple locations on the tissue at
high resolution without disturbing
recording electrodes. The MT-1000
workstation encompasses: an X-Y
translator designed to move a microscope smoothly and accurately;
two MT-75 gantry-type stands for
positioning manipulators on one or
both sides of the microscope; and
a third gantry stand that becomes
the fixed stage for the microscope.
10/3/05 5:06:52 PM
MICROMANIPULATION
35
MPC-2OO/MPC-385/MPC-325
M U LT I - M I C R O M A N I P U L AT O R S Y S T E M S
(Shown: MPC-385-2)
Neurobiological experiments are becoming more complex. Many require
multiple manipulators with control
units that quickly become space and/
or cost prohibitive. The MPC-200 is
the solution you have been asking for.
A single controller capable of running
2 manipulators! Sutter Instrument
has taken the simplicity of the MP225 controller and expanded it to run
two manipulators from a single controller/ROE. The MPC-200 works with
one or two of our world-renowned
mechanicals, the MP-285(MPC-385)
or the MP-225 (MPC-325).
If two manipulators aren’t enough,
a second controller can be daisy
chained to allow the single ROE-200
to move up to four manipulators.
Thus the system can be easily expand-
ed to control highly sophisticated
experiments.
The Sutter MPC-200 is electrically
quiet. Unique to the MPC-200 is our
multi-unit controller which employs
linear output circuitry to minimize
electrical noise. Sutter adds additional manipulators to the same controller without requiring potentially
noisy chopper drives.
Faster automated pipette exchange.
The MPC-200 has faster “Home” and
“Work Position” moves for quicker pipette exchange. Automation is set up
and run via the same interface used in
the MP-225 controller; however, the
speeds of the automated movement
are much faster.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 34-35
Accelerated manual mode.
For users who prefer manual pipette
exchange we have added “Accelerated Mode” to the ROE. Using Accelerated Mode, the user can make
quick manual moves in and out of a
setup. Accelerated Mode amplifies
the speed attainable in a manual
move by smoothly accelerating to the
maximum speed during sustained,
fast turns of the ROE. Accelerated
movement ends as soon as the user
stops turning the knob. This mode
can be fully disabled for those who
feel the need for direct control of
the pipette.
To provide the ultimate in flexibility, the MPC-200 can be purchased
separately or as basic systems such
as the MPC-385 and MPC-325. Additional component pieces can be
added at any time, allowing the user
to develop a system tailored to his or
her particular needs. The controller
is self-detecting so there is no need
for manual configuration of the
components.
F E AT U R E S
> MPC-200
Single controller and ROE will
run two stepper motor drive
manipulators
Self-detecting, daisy-chain
capability allows control of
four manipulators from one
ROE-200
MICROMANIPULATION
34
User-friendly interface: single
button access to all major functions
New Accelerated Mode for fast,
manual manipulator movement
Easy toggle selection of Mode
(speed/resolution, pulsed diagonal, Accelerated Mode)
Display indicates X, Y, Z coordinates, Mode, active
manipulator
Robotic Home and Work
Position moves for easy
automated pipette exchange
Faster robotic moves than previous versions
Definable 4th axis for coaxial
pipette movement, angle selected by DIP switches
Simple USB interface
Toggle switch selects which
manipulator is connected to
input device
LED and display indicate active
manipulator
Drift-free mechanical stability
(Patents pending)
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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10/3/05 5:06:56 PM
MICROMANIPULATION
37
US PRICES
> MPC-200
International prices are 5% higher.
Prices subject to change without notice.
MPC-325
One MP-225 manipulator mechanical, one MPC-200 controller and
one ROE-200. Also includes mounting adapter plate, rod holder, 4 inch
dovetail extension, manipulator and ROE connecting cables, power
cord and manual.*
$ 7,450
MPC-385
Same as above, but with one MP-285 manipulator mechanical
substituted for the MP-225 mechanical. *
$ 8,500
MPC-325-2
Same as the MPC-325, but with two MP-225 manipulator
mechanicals. *
$ 11,900
MPC-385-2
Same as the MPC-385, but with two MP-285 manipulator
mechanicals. *
$ 13,900
MPC-200-ROE
Consists of the MPC-200 controller and ROE-200. This “system” is made
available at a special price for those who wish to use previously purchased Sutter manipulators with the newer controller system or to use
the MPC-200-ROE to control other devices. Includes cable to connect
ROE to controller, power cord and manual.
$ 3,500
* Indicate right- or left-handed set-up when ordering
COMPONENTS
MPC-200
Controller box alone. Purchase this if you intend to daisy-chain two
controllers to one ROE-200. In this configuration, up to 4 manipulators can be controlled by a single ROE-200. NOTE: This is not a standalone device. In order to function, the MPC-200 must be connected to
a second MPC-200 with ROE-200 attached or be connected to its own
ROE-200. Includes daisy chain cable, power cord and manual.
$ 2,800
ROE-200
This is the ROE alone. Provides user input to one or more MPC-200
controllers. This component is intended for users who have an existing
four-manipulator MPC-200 system with a single ROE-200 and wish to
split it into two independent two-manipulator systems. Includes cable
to connect ROE to controller.
$1,250
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 36-37
US PRICES (C0NTINUED)
> MPC-200
MP-285/M
The MP-285 manipulator
mechanical alone. This is the
mechanical from the MP-285,
the manipulator system that
made Sutter the world leader
in motorized manipulators for
electrophysiology. Over 2000
units, many of which have
been in use over 5 years, allow
us to stand behind the reliability of this design. It is widely
known for its smooth movement and low drift. Includes
mounting adapter plate, rod
holder, 4in. dovetail extension,
hinged headstage mount and
cable to connect manipulator
to controller.* $ 5,500
MICROMANIPULATION
36
MP-225/M
The MP-225 manipulator
mechanical alone. This is the
mechanical unit from the
Sutter MP-225. It features
modular construction, lower
cost, smooth movement and
low drift. Includes mounting
adapter plate, rod holder,
4 inch dovetail extension,
hinged headstage mount and
cable to connect manipulator
to controller.* $ 4,750
* Indicate right- or left-handed
set-up when ordering
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:01 PM
MICROMANIPULATION
39
US PRICES
> MPC-200
International prices are 5% higher.
Prices subject to change without notice.
ACCESSORIES
X285204
X285210*
285RBI
225RBI
X285300
X285305
X285310
4 inch dovetail extension
Mounting adapter plate
Rotating base for MP-285
Rotating base for MP-225
Right angle adapter
Z-axis vertical extension
Z-axis horizontal extension
$
$
$
$
$
$
$
37
48
315
315
48
48
48
For detailed information on mounting our micromanipulators, refer to the
Mounting Systems section or phone for assistance.
* For use with MT and MD series stands and platforms, or any surface with
1 inch centered holes.
S P E C I F I C AT I O N S
> MPC-200
Resolution and Full Travel
Minimal microstep size is 62.5
nanometers per microstep.
Display has single micron resolution. Full travel is 25mm
in each axis.
MICROMANIPULATION
38
Maximum Speed
MP225
mechanical 3mm/sec.
MP285
mechanical 5mm/sec.
DRIFT
< 1 micron/hr
Drive Mechanism
DIMENSIONS
Controller
16in x11in x 3.5in
41cm x 28cm x 9cm
ROE
10in x 6in x 4in
25cm x 15cm x 23cm
WEIGHT
Controller
25lbs 11kg
ROE
3.5lbs 1.6kg
Electrical
115/230 Volts
50/60 Hertz power line
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 38-39
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:01 PM
MICROMANIPULATION
41
MP-285
M O T O R I Z E D M I C R O M A N I P U L AT O R
(Shown with ROE input device)
able for patch clamp experiments, extracellular recording, microinjection,
intra-cellular recording and precision
robotic positioning applications. An
outstanding feature of this system is
the unique definable 4th axis for diagonal advancement of the pipette.
You select the angle, then activate
the 4th axis. As with the other three
axes, you may move with adjustable
coarse or ultrafine resolution, select
the movement speed, and move continuously or in single step increments.
To quickly reposition the pipette,
simply select the Home function. Axes
positions are continuously shown in
relative and absolute scales, and are
easily readable on the vacuum fluoThe MP-285 was designed to meet a rescent display.
wide variety of positioning needs for
the scientific community, and is suit-
The flagship in our line of precision
micromanipulators, the motorized
MP-285 is affordable yet offers
advanced features found in manipulators costing thousands more.
Custom engineered stepping motors,
precision cross-roller bearing slides
and proprietary worm gear capstan
drives form the basis of the watch-like
mechanical system. The controller
provides power to the stage motors
with a quiet linear power supply to
minimize electrical noise radiation
in your setup. Pipette holders and
headstages are securely mounted to
the MP-285 with one of our several
unique and rigid mounting systems.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 40-41
The extremely low backlash of the
MP-285 removes traditional drawbacks of “open loop” technology and
eliminates drift. This allows submicron resolution down to 0.2 microns
in the coarse range and down to 40
nanometers in the fine range. With
over 1 inch of motorized travel on all
three axes, and a user designated 4th
axis, the MP-285 allows tremendous
range of motion while maximizing
resolution.
Available with a table-top or rack
mounted controller, our manipulator
fits in seamlessly with your other components while the compact design
and assignable axes of the MP-285
allows you to easily integrate it into
your setup at any orientation. To
add to its practicality, your choice of
one of two manual controls: joystick
or rotary optical encoder (ROE), assures a comfortable experimentation
environment, customized to the
scientist.
For users who require repeatable motion sequences, the MP-285 features
easily programmed robotic control
from the keypad, or via a remote
computer. The system can store up
to 500 position instructions, including pauses, and will execute the
instruction set once, continuously, or
in reverse.
F E AT U R E S
> MP-285
Highly stable for experiments
intolerant of pipette drift.
Submicron resolution and integrated coarse positioning.
MICROMANIPULATION
40
1 inch of motorized travel on
all three axes.
4th axis with user-selected
angle for axial drive.
Adjustable speed and resolution allows optimization for
your experimental setup.
Programmable robotics for
complex motion sequences.
Continuous display (in microns)
of axes positions.
Switch between continuous or
single step movement.
Absolute and relative origins.
Convenient Home function
allows pipettes to be quickly
repositioned.
Assignable axes permit any orientation of the manipulator.
Easy to read vacuum fluorescent display.
Remote computer control via
serial interface.
Compact design easily adaptable to your setup.
Universal mounting system for
headstage or pipette holder.
Optional mounting adapters
(see price list).
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:02 PM
MICROMANIPULATION
43
As always, our technical support team
is available to address your concerns
and answer all questions before, and
after your purchase.
CHOOSE ONE OF TWO INPUT
DEVICE OPTIONS
1) Rotary Optical Encoder (ROE).
Turning one of three 2-inch knobs
produces a movement along one
axis proportional to the amount and
speed of the turn. Buttons allow
the activation of “4th axis”, change
of movement resolution, “home”
return function, and toggle between
continuous pulse movements.
2) Joystick. Our joystick is a modified three-axis game controller. The
degree of handle deflection determines the velocity of movement of
each axis. In pulse mode, a button
commands the manipulator to take
a single step in the axis selected by
joystick deflection.
International prices are 5% higher.
Prices subject to change without notice.
S P E C I F I C AT I O N S
> MP-285
US PRICES
> MP-285
Travel
1 inch / 25mm on all three axes
MP-285/R Includes manipulator, rack mount controller unit, cables,
rod holder, 4in dovetail extension, mounting adapter plate, choice of
input device, power cord, and manual
$ 7,900
Resolution
Low: 0.2µm/step
High: 0.04µm/step
Maximum Speed
2.9mm/sec
Long Term Stability
<10nm/hour at 24deg C.
Drive Mechanism
Precision worm gear
capstandrive
Serial Interface
RS-232, 9600 baud
(1 start bit, 8 data bits,
1 stop bit)
Dimensions
Mechanical: 4.5in x 6in x 6.25in
11cm x 15cm x 16cm
Controller: 4in x 16in x 12.25in
10cm x 40.5cm x 31cm
MP-285/T Includes manipulator, table top controller unit, cables,
rod holder, 4in dovetail extension, mounting adapter plate, choice
of input device, power cord, and manual
$ 7,900
MP-285/3Z Includes 3 independant Z axes with extra load carrying
capacity. 22mm travel in each axis. Paired with standard MP-285
controller and ROE. Designed to provide 3 axes of movement in a
moveable objective microscope. Not intended to be used as a micromanipulator!
$ 9,900
MICROMANIPULATION
42
When ordering the MP-285, the following items must be specified.
Please list as seperate items with no cost.
Choice of one input device
Rotary Optical Encoder
Joystick
285ROE
285JOY
The handedness of the manipulator
Right handed setup
Left handed setup
RIGHT
LEFT
If you have any questions regarding the part numbering system, please
contact Sutter Instrument Company directly.
ACCESSORIES
Weight
Manipulator: 3.85lb/1.7kg
Controller: 10lb 11oz/4.5kg
Electrical
115/230 Volts
50/60 Hertz power line
X285204
X285210*
285RBI
X285300
X285305
X285310
4 inch dovetail extension
Mounting adapter plate
Rotating base for MP-285
Right angle adapter
Z-axis vertical extension
Z-axis horizontal extension
$
$
$
$
$
$
37
48
315
48
48
48
For detailed information on mounting our micromanipulators, refer to the
Mounting Systems section or phone for assistance.
* For use with MT and MD series stands and platforms, or any surface with
1 inch centered holes.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 42-43
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:03 PM
MICROMANIPULATION
45
MECHANICAL DRAWINGS MP-285
MECHANICAL DRAWINGS OPTIONAL ROTATING BASE
(285RBI OR 225RBI)
723
723
MICROMANIPULATION
44
$//0($685(0(176$5(,1,1&+(6
$//0($685(0(176$5(,1,1&+(6
)5217
6,'(
6,'(
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 44-45
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:03 PM
MICROMANIPULATION
47
MP-225
M O T O R I Z E D M I C R O M A N I P U L AT O R
The MP-225 is one of the newer
products in the Sutter Instrument
Company micromanipulator line.
Production and design changes have
allowed us to produce a motorized
manipulator that is a more affordable
alternative to the industry standard
MP-285. While the MP-225 feature
set is less comprehensive than the
MP-285, it includes the most popular
features with a simpler user interface.
The mechanical design utilizes a miniature stepper motor and integral
anti-backlash gear head. Pre-loaded
ball bearing slides provide smooth
movement throughout the 25mm of
travel. The controller uses low-noise,
linear-drive output circuitry identical
to that found in the MP-285. The
methodology for mounting pipette
holders and headstages used with
the controller can be moved to a less
accessible area of your setup and does
not need to occupy prime space in an
equipment rack.
As always, our technical support team
is available to address your particular
needs and answer all questions before and after your purchase.
the MP-285 has been maintained in
the MP-225 to allow for cross compatibility.
The MP-225 is designed primarily for
positioning patch and intracellular
recording pipettes. We have retained
and refined the features most desired
for this type of work. An extended
version of the popular rotary optical encoder (ROE) is the sole input
device available with the MP-225.
Like the MP-285, the manipulator
has a synthetic 4th axis for diagonal
advancement of the pipette; 16 different angles are selectable via DIP
switches. Speed and resolution of
movement are easily selected with
a multiple position thumbwheel,
allowing fast/course movement and
slow/ultra-fine movement in 10 incre-
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2006_catalog.indd 46-47
ments. Two commonly used robotic
movements have been incorporated
for user convenience. A single button press can initiate a move to a
Home position for pipette exchange
or to a user defined Work position
for quick location of the pipette near
the recording location. A display on
the ROE gives position location. As
all controls are located on the ROE,
MICROMANIPULATION
46
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
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F E AT U R E S
> MP-225
S P E C I F I C AT I O N S
> MP-225
US PRICES
> MP-225
Highly stable for experiments
intolerant of pipette drift.
Travel
25mm on all three axes
U.S. PRICES
Submicron (62.5nm) minimal
resolution for fine movement.
Resolution
Six microstep sizes selectable
(µm/ustep): 0.0625, 0.125,
0.25, 0.5, 1.0 and 2.0.
International prices are 5% higher.
Prices subject to change without notice.
Motorized Micromanipulator
Convenient thumbwheel selects
resolution/speed of movement.
25mm of motorized travel on
all three axes.
4th axis for coaxial movement
of pipette, angle selected by
DIP switches on ROE.
ROE button press actuates
move to Home position for
pipette exchange.
ROE button press actuates
move to Work position near
recording location.
Continuous display (in microns)
of axes positions located on
ROE.
DIP switches on ROE select direction of movement produced
by turn of ROE knob.
Modularized, compact design
easily adaptable to your setup.
Universal mounting system for
headstage or pipette holder.
Mounting adapters included
with manipulator.
Finer movement settings use
the 62.5nm microstep size but
fewer microsteps are commanded per encoder knob
turn
Maximum Speed
2.0mm/sec
Long Term Stability
1-2 µm/hour maximum
Drive Mechanism
Integral miniature stepper motor anti-backlash gearhead
Dimensions
Mechanical: 4in x5.5in x 6in
10cm x 15cm x 15.5cm
For detailed information on mounting our micromanipulators,
refer to the Mounting Systems section or phone for assistance.
* Indicate right- or left-handed set-up when ordering.
ACCESSORIES
X285204
X285210*
225RBI
X285300
X285305
X285310
4 inch dovetail extension
Mounting adapter plate
Rotating base for MP-225
Right angle adapter
Z-axis vertical extension
Z-axis horizontal extension
$
$
$
$
$
$
37
48
315
48
48
48
* For use with MT and MD stands and platforms, or any surface with one inch
centered holes.
Controller: 4in x 16in x 12.25in
10cm x 40.5cm x 31cm
Weight
Manipulator: 2.95lbs /1.3kg
Controller: 10lb 11oz/4.5kg
Electrical
115/230 Volts
50/60 Hertz power line
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2006_catalog.indd 48-49
MP-225 Includes manipulator, table top controller unit, extended
ROE, cables, rod holder, 4in dovetail extension, mounting adapter
plate, screws, hex wrench, power cord and manual*
$ 6,500
MICROMANIPULATION
48
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
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MP-85
H U X L E Y- W A L L T Y P E M I C R O M A N I P U L AT O R
F E AT U R E S
> MP-85
OPTIONS
> MP-85
Dual springing of the moment
arms to insure zero backlash
and zero torsion.
Fifteen degree stackable wedges for tilting the manipulator.
The micromanipulator is
mounted on a precision rotating base featuring a positive
stop and lock.
A set of three magnetic feet for
increased stability.
MICROMANIPULATION
50
One inch thick, chrome plated,
solid brass riser blocks.
The coarse movement is an
all cross-roller bearing design
consisting of coarse X, Y, and Z
with an additional fine X motion.
The micromanipulator can be
ordered in either a right or left
handed version.
(Shown with optional spacer, wedge and magnetic feet)
The classic micromanipulator developed by Sir Huxley many years ago is
still considered by many investigators
to be the finest manual micromanipulator available. The MP-85 offers
the advantage of a very large range
of movement with its built-in coarse
manipulator and precise submicron
movement with the fine controls.
Coarse positioning is achieved with
a three axis manipulator mounted
on top. The ultrafine positioning is
accomplished with the large micrometers mounted on the base. These
micrometers provide smooth, precise
movement through a 10:1 reduction
mechanism.
with excellent damping properties.
This exceptionally stable design is
ideal for patch clamp recording, intracellular recording, or any other application that is intolerant of drift.
The MP-85 is chrome-plated and anodized to prevent corrosion. The J.R.
Wall designed rotating base is machined from solid stainless steel with
PTFE
a brass spindle; a thin Tefl
on washer
provides for the bearing surface.
Please contact Sutter Instrument
for any additional information on
the suitability and use of the MP-85
micromanipulator for your specific
application.
The brass and stainless steel construction of the MP-85 makes for a very
heavy and solid micromanipulator
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2006_catalog.indd 50-51
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
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S P E C I F I C AT I O N S
> MP-85
US PRICES
> MP-85
Coarse X-axis tilt
0 to 45 degrees
in 15 degree increments
M I C R O M A N I P U L AT O R
Coarse resolution
0.1mm on all axes
Fine X resolution
0.01mm
Ultrafine (Huxley)
resolution
0.2µm
Huxley excursion
~2mm maximum in each axis
Dimensions
10in x 10in x 12in
25cm x 25cm x 28cm
International prices are 5% higher.
Prices subject to change without notice.
MP-85/R -Right handed- Includes manipulator, coarse movement
MM-33 micromanipulator, and rotating base
$ 4,500
MICROMANIPULATION
52
MP-85/L -Left handed- Includes manipulator, coarse movement
MM-33 micromanipulator, and rotating base
$ 4,500
ACCESSORIES
Holder/MP85
RBI
T363410
85AX200
X850600
X850700
X700115
Universal headstage adapter
Rotating base forMP-85
Magnetic feet (set of 3)
Axiopatch 200/Heka EPC-10 adapter
15 degree wedges (each)
1 inch increment spacer
(No more than three recommended)
MP-85 to MT stand adapter plate
$
$
$
$
$
$
141
450
33
215
64
64
$ 37
Weight
15lbs
7kg
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
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2006_catalog.indd 52-53
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
55
fine axis to be tilted up to 45 degrees.
An optional vertical lock is also available for locking the position of the
vertical axis. These options should
be ordered at the time of purchase
since the modifications are factory
installed.
MM-33
M I C R O M A N I P U L AT O R
INDIVIDUAL EXCURSIONS
ARE AS FOLLOWS:
X-axis coarse movement:
37mm
X-axis fine movement:
10mm
MICROMANIPULATION
54
Y-axis horizontal movement:
20mm
Z-axis vertical movement:
20mm
(Shown: MM-33A/R)
Successfully used in conjunction with
our other micromanipulators, the
MM-33 is available separately for
those wishing a small, practical instrument in applications not requiring
submicron accuracy.
stable, drift-free movement with
minimal backlash. The controls are
placed one above the other which
makes for less hand movement and
easier positioning. The scales for
all axes are calibrated in 0.10mm
increments and the fine micrometer
The MM-33 is the right choice for adjustment for the X-axis thrust is
tight environments which require calibrated at 0.01mm with estimates
maximum versatility within a small to 5um.
space. The optional rotating base and
tiltable X-axis provide even further The optional rotating base extends
flexibility.
the utility of the MM-33 by providing two additional rotating planes.
Stainless steel cross-loaded roller The rotation in the horizontal plane
bearings are designed to offer low (approximately 120 degrees) has a
friction and smooth linear motion positive stop, quick release mechawith a minimum of side play. The pre- nism for fast insertion and removal
loaded rollers are set at 90 degrees to of pipettes. The second rotating axis
the hardened steel guides insuring allows the MM-33 to be tilted in a
constant contact and accuracy. This vertical plane from 0 to 70 degrees.
precise rack and pinion drive gives The optional tiltable X-axis allows the
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2006_catalog.indd 54-55
F E AT U R E S
> MM-33
OPTIONS
> MM-33
Compact design allows use in
tight environments.
Rotating base allows horizontal
rotation and vertical tilting.
Cross-loaded roller bearings for
smooth, low friction movement.
Rotating base allows horizontal
rotation and vertical tilting.
Rack and pinion drive gives
stable movement with minimal
backlash.
Vertical axis lock.
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
57
International prices are 5% higher.
Prices subject to change without notice.
US PRICES
> MM-33
MICROMANIPULATOR
MM-33/R Right handed micromanipulator
(with 1/2 inch rod clamps*)
$ 950
MM-33/L Left handed micromanipulator
(with 1/2 inch rod clamps*)
$ 950
MICROMANIPULATION
56
MM-33A/R Right handed micromanipulator with tilting mount for
fine X-axis, vertical lock, rotating base, and two base clamps
$ 1,695
MM-33A/L Left handed micromanipulator with tilting mount for
fine X-axis, vertical lock, rotating base, and two base clamps
$ 1,695
(*12mm rod clamps available upon request)
ACCESSORIES
MM-330001
Right-handed tilting mount for fine x-axis $ 136
MM-330011
Left-handed tilting mount for fine x-axis
$ 136
MM-33002
Rotating base with two base clamps
$ 370
X8504451
Vertical lock
$ 137
X330105
Base clamps (2) for clamping
rotating base
$ 32
Stationary mounting base
$ 105
X3301031
1
Must be ordered with the MM-33 since this option is factory installed only.
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2006_catalog.indd 56-57
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
59
MM-1 & MM-3
MICROPOSITIONERS
loaded stages move on stainless steel
tracks for smooth motion and precision; maximum wobble and straightness on the MM-1 and MM-3 is less
than 1µm and 2µm, respectively.
Both models are available in single,
double, or triple stages for X, Y, and Z
axes; adding stages is an easy job and
requires no special tools or adaptors.
The travel of the stages is 1/8 inch
(3mm) for the MM-1 and 1/2 inch
The black anodized aluminum body (13mm) for the MM-3. The base is
is made with a one-piece base and easily adapted to most surfaces.
three piece construction. The springThese unique micropositioners are
precision instruments designed to
eliminate many research and design
problems at a justifiable cost, especially in the case of space limitations.
The MM-3 is roughly 1 3/4 inches
(4.4cm) in height and less than 1 inch
(2.5cm) in width and depth. The MM1 is even smaller at a height of only
1 inch (2.5cm).
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2006_catalog.indd 58-59
S P E C I F I C AT I O N S
> MM-1
S P E C I F I C AT I O N S
> MM-3
Dimensions
0.20in x 0.44in x 1.03in
5mm x 11mm x 26mm
Dimensions
0.28in x 0.66in x 1.75in
7mm x 17mm x 44mm
Travel
1/8in
3mm
Travel
1/2in
13mm
Straightness
less than 1µm
Straightness
less than 2µm
Wobble
less than 1µm
Wobble
less than 2µm
Screw adjustments
64 TPI
Screw adjustments
40 TPI
Thermal stability
60deg F to 165deg F
Thermal stability
60deg F to 165deg F
MICROMANIPULATION
58
International prices are 5% higher.
Prices subject to change without notice.
>U SMPMR -I 1C E S
US PRICES
> MM-1
MM-1 X
Single stage
$ 131
MM-1 XY
Double stage
$ 273
MM-1 XYZ
Triple stage
$ 430
US PRICES
> MM-3
International prices are 5% higher.
Prices subject to change without notice.
MM-3 X
Single stage
$ 136
MM-3 XY
Double stage
$ 292
MM-3 XYZ
Triple stage
$ 452
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
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MECHANICAL DRAWINGS MM-1 TRIPLE STAGE
MECHANICAL DRAWINGS MM-3 TRIPLE STAGE
723
MICROMANIPULATION
60
723
$//',0(16,216$5(,1,1&+(6
$//',0(16,216$5(,1,1&+(6
)5217
6,'(
)5217
6,'(
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2006_catalog.indd 60-61
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
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MICROMANIPULATION
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S P E C I F I C AT I O N S
> M T- 2 0 0 0 X - Y T R A N S L A T O R
M T- 2 0 0 0
M O T O R I Z E D X - Y T R A N S L AT O R S Y S T E M F O R
F I X E D - S TA G E M I C R O S C O P E S
Baseplate Dimensions
8in x 15.25in x 2.25in
200mm x 375mm x 55mm
Maximum Travel
22-23mm
US PRICES
> M T- 1 0 0 0
The MT-2000 is a motorized microscope translator system including
fixed-stage and manipulator supports. The modular design of the
MT-2000 features free-standing columns that provide rock solid mounting for the tissue recording chamber
and multiple micromanipulators. A
two-axis rotary optical encoder (ROE)
accepts user input to the motorized
translator and can be placed in any
convenient location in your setup.
addition, the MT-800/2000 controller gives the user robotic control of
movement and a serial computer
interface.
MT-800 motorized translators are
currently available for the Olympus
BX-51WI, Nikon E600FN, Zeiss Axioskop 2 FS and the Leica DMLFS.
In the complete system, MT-2000, the
recording chamber and micromanipulators are mounted on independent
The core of the MT-2000 system is columns firmly affixed to the users vithe MT-800 stepper-motor driven bration isolation table. The chamber
X-Y translation table. The motors column options are also available.
are powered by a two-axis version
of the popular MP-285 manipulator
controller. As in the MP-285, the controller and ROE give the user smooth,
high-resolution control of motion. In
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2006_catalog.indd 62-63
Resolution
0.250µm course/0.050µm fine
Maximum Load
110lbs /50kg
Weight
35 lbs/16kg
MICROMANIPULATION
62
Mechanical controller
11 lbs/4.5Kg
International prices are 5% higher.
Prices subject to change without notice.
MT-2000/Y51* Complete system for the Olympus BX51WI
includes: Motorized X-Y translator, chamber
column, two micromanipulator columns $ 9,900
MT-2000/Z25* Complete system for the Zeiss Axioskop 2 FS
includes: Motorized X-Y translator, chamber
column, two micromanipulator columns $ 9,900
MT-2000/N60* Complete system for the Nikon E600FN
includes: Motorized X-Y translator, chamber
column, two micromanipulator columns $ 9,900
MT-2000/L30* Complete system for the Leica DMLFS includes:
Motorized X-Y translator, chamber column,
two micromanipulator columns
$ 9,900
*Please specify chamber type when ordering.
TRANSLATORS
MT-800/Y51
MT-800/Z25
MT-800/N60
MT-800/L30
Motorized X-Y translator for
Olympus BX51WI
Motorized X-Y translator for
Zeiss Axioskop 2 FS
Motorized X-Y translator for
Nikon E600FN
Motorized X-Y translator for
Leica DMLFS
$ 7,900
$ 7,900
$ 7,900
$ 7,900
CHAMBER COLUMN
MT-150/W20
Chamber column for Warner Instruments
Series 20 recording chamber
$ 730
MT-150/CUST
Chamber column for non-standard
recording chamber
$ 830
MANIPULATOR COLUMNS
MT-75
Tall gantry micromanipulator stand
MT-75S
Short gantry micromanipulator stand
$ 730
$ 730
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
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M T- 1 0 0 0
X - Y T R A N S L AT I O N S Y S T E M F O R
F I X E D - S TA G E U P R I G H T M I C R O S C O P E S
The MT-1000 is a microscope translator, fixed-stage and manipulator platform for moving optical
pathway electrophysiology. The
modular design of the MT-1000
features free-standing columns that
provide rock solid mounting for the
tissue recording chamber and multiple micromanipulators, such as the
MP-285 or MP-225. The columns are
positioned around a movable table
that provides X-Y translation of the
optical pathway in order to view multiple locations within the chamber at
high resolution.
The column-mounted recording
chamber is relatively compact and
serves as an alternative to bulky
stages. The modular columns allow
easy access to the microscope and
provide a very flexible design plat-
form for customizing your rig.
The recording chamber and micromanipulators are mounted on
independent columns firmly affixed
to the user’s vibration isolation
table. The manipulator columns
are identical to the popular MT-75
gantry style stands. The recording
chamber fits into a dovetail slide on
a similar column. While the chamber
column shown conforms to a Warner
Instruments Series 20 design, other
chambers can be accommodated. A
dovetail slide with a generic opening is available for users who wish to
develop custom chambers.
S P E C I F I C AT I O N S
> M T- 5 0 0 X - Y
T R A N S L AT O R
Baseplate Dimensions
8in x 15.25in x 2.25in
200mm x 375mm x 55mm
Maximum Travel
1in
25mm
Resolution
0.0002in
5µm
Maximum Load
110lbs
50kg
Weight
35 lbs
16kg
At the core of the system is the X-Y
translation table. It is designed to
allow movement of a microscope’s
optics with respect to the fixed stage.
Such an arrangement is currently in
use by electrophysiologists recording
from tissue slice preparations. The
translator allows visualization and/or
imaging of multiple tissue locations
while maintaining multiple stable recordings from the preparation. Such a
system is also useful for experiments
on cells in culture where one wishes
to monitor several cells not in the
same field of view, e.g., recording
from pre and post synaptic neurons
in culture and/or imaging one cell
while recording from or stimulating
another.
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2006_catalog.indd 64-65
Translators are available for the Olympus BX-51WI, Nikon E600FN, Zeiss
Axioskop 2 FS and the Leica DMLFS
as well as older fixed stage models.
A generic rectangular table (295mm
x 340mm) which can accommodate
most standard, upright microscopes
as well as a number of inverted microscopes is also available.
MICROMANIPULATION
64
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
67
US PRICES
> M T- 1 0 0 0
International prices are 5% higher.
Prices subject to change without notice.
MT-1000/Y50* Complete system for the Olympus BX50WI includes:
X-Y translator, chamber column, two micromanipulator columns.
$ 5,900
MT-1000/Y51* Complete system for the Olympus BX51 includes:
X-Y translator, chamber column, two micromanipulator columns
$ 5,900
MT-1000/N60* Complete system for the Nikon E600FN includes:
X-Y translator, chamber column, two micromanipulator columns.
$ 5,900
MT-1000/L30* Complete system for the Leica DMLFS includes:
X-Y translator, chamber column, two micromanipulator columns
$ 5,900
MT-1000/Z20* Complete system for the Zeiss Axioskop FS includes:
X-Y translator, chamber column, two micromanipulator columns
$ 5,900
MT-1000/Z25* Complete system for the Zeiss Axioskop 2 FS
includes: X-Y translator, chamber column, two
micromanipulator columns
$ 5,900
MICROMANIPULATION
66
*Please specify chamber type when ordering.
COMPONENTS
TRANSLATORS
MT-500/Y50
MT-500/Y51
MT-500/N60
MT-500/L30
MT-500/Z20
MT-500/Z25
X-Y translator for Olympus BX50WI
X-Y translator for Olympus BX51
X-Y translator for Nikon E600FN
X-Y translator for Leica DMLFS
X-Y translator for Zeiss Axioskop FS
X-Y translator for Zeiss Axioskop 2 FS
$
$
$
$
$
$
3,900
3,900
3,900
3,900
3,900
3,900
CHAMBER COLUMN
MT-150/W20
Chamber column for Warner Instruments Series
recording chamber
$ 730
MT-150/CUST
Chamber column for non-standard
recording chamber
MANIPULATOR COLUMNS
MT-75
Tall gantry micromanipulator stand
MT-75S
Short gantry micromanipulator stand
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
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2006_catalog.indd 66-67
$ 830
$ 730
$ 730
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
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MICROMANIPULATION
69
M T- 7 0 S E R I E S
M I C R O M A N I P U L AT O R S TA N D S
adapter plate at the time of purchase.
Larger baseplates or mounting plates
are available upon special order.
S P E C I F I C AT I O N S
> M T- 7 0 S E R I E S
Dimensions of Baseplate
6in x 6in x 3/4in
153mm x 153mm x 19mm
MICROMANIPULATION
68
Dimensions of
Mounting Plate
5in x 5in x 5/16in
128mm x 128mm x 8mm
Total Stand Height
4 1/16in to 13 1/16in
104mm to 333mm
depending on configuration
Weight
3lb 11oz to 8lb 5oz
1.7kg to 3.8kg
depending on configuration
The MT-70 series of stands are sturdy,
stable, and extremely versatile for
mounting components for a variety
of research applications. They were
designed for placing our heavy Huxley-style micromanipulators adjacent
to any microscope. We found them to
be so useful and adaptable that we
also use them for mounting other
items such as optical components.
The stands are based on a rigid
extruded aluminum tower which is
available in 3 inch increments up to
12 inches. The tower is mounted on
a sturdy 3/4in thick aluminum baseplate and then fitted with a 5/16in
thick aluminum mounting platform.
These systems are configurable as
fixed height stands or, in the MT-71
series, have an adjustable height
cantilevered side platform for added
versatility. The tower can be center
or edge mounted to the baseplate
for accommodating various spatial
layouts. Rows of 1 inch and 25mm
centered clearance holes are provided in the baseplate for attachment
to tables. The mounting platforms
have tapped 1/4-20 mounting holes
on 1 inch centers.
The stands are available in sets, or
you can order individual components
separately to meet your specific requirements. Custom heights can be
made to order for an additional fee.
To securely fasten our MP-85 Huxley
style manipulator to the mounting
plates, please order the manipulator
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
WEB: WW W .S U TTER .C OM
2006_catalog.indd 68-69
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
71
MECHANICAL DRAWINGS MT-70-3
US PRICES
> M I C R O M A N I P U L AT O R
S TA N D S
International prices are 5% higher.
Prices subject to change without notice.
M T- 7 0 S E R I E S
MT-70-3
3in tower, baseplate, mounting platform
and fastening hardware
Same as above with 6in tower
Same as above with 9in tower
Same as above with 12in tower
MT-70-6
MT-70-9
MT-70-12
$
$
$
$
280
310
340
370
MICROMANIPULATION
70
$//0($685(0(176$5(,1,1&+(6
(Custom tower lengths available: $20 per inch, $25 per cut)
M T- 7 1 S E R I E S
MT-71-3
3in tower, baseplate, cantilever assembly
with mounting platform,tower endcap and
fastening hardware
$
Same as above with 6in tower
$
Same as above with 9in tower
$
Same as above with 12in tower
$
MT-71-6
MT-71-9
MT-71-12
340
370
400
430
(Custom tower lengths available: $20 per inch, $25 per cut)
MECHANICAL DRAWINGS MT-71-6
ACCESSORIES
MT-7001
T363400
X700100
X700102
X700115
Cantilever mounting assembly
Magnetic feet (set of 4)
Baseplate—6in x 6in x 3/4in thick
Mounting plate—5in x 5in x 5/16in thick
MP-85 adapter plate
$
$
$
$
$
145
44
110
80
37
$//0($685(0(176$5(,1,1&+(6
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
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2006_catalog.indd 70-71
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
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MICROMANIPULATION
73
M T- 7 5 S E R I E S
G A N T R Y S T Y L E M I C R O M A N I P U L AT O R S TA N D
microelectrodes from headstages).
Remove the positive stop and you
have a full 360 degree rotation. A
PTFE washer contributes to the
Teflon
smooth rotation of the system.
S P E C I F I C AT I O N S
> M T- 7 5 S E R I E S
The solid aluminum baseplate has
through-holes on 1inch (25mm) centers for mounting onto tables. The
cantilever has eighteen 1in spaced
10-32 holes along the edges.
Dimensions of Baseplate
6in x 6in x 3/4in
153mm x153mm x 19mm
Dimensions of Cantilever
2 1/2in x 8in x 3/4in
64mm x 204 x 19mm
MICROMANIPULATION
72
Total Stand Height
MT-75
Adjustable 8 5/8in to 12 1/2in
220mm to 319mm
MT-75S
Adjustable 6 5/8in to 9 1/2in
169mm to 242mm
Weight
MT-75 11lb 7oz
(5.2kg)
MT-75S 9lb 14oz
(4.5kg)
For ultimate flexibility in positioning
micromanipulators or other devices
adjacent to your microscope, look to
the MT-75 series stands. These unique
gantry systems offer adjustable vertical and horizontal axes and up to 360
degrees of rotation. Your manipulator or other device will mount to the
3/4 inch thick aluminum cantilever
which is dovetailed for secure positioning. The cantilever assembly is
mounted on a heavy 2 inch diameter
stainless steel post. The post is supported within the same extruded
aluminum tower used in our MT-70
stands. The tower can be center
mounted or edge mounted to the
base-plate for an additional degree
of positioning freedom.
an aluminum collar which securely
locks onto the steel post to fix the
height of the system. The MT-75 is
suitable for use with most inverted
microscopes, and can be adjusted
from a minimum height of 8 5/8
inches to a maximum height of 12
1/2 inches The MT-75S is suitable
for most upright scopes, and can
be adjusted from 6 5/8in to 9 1/2in.
These systems are very stiff, deflecting approximately 2µm per 25mm
rise with a 1.5 kg load at the end of
the cantilever.
US PRICES
> M T- 7 5 S E R I E S
International prices are 5% higher.
Prices subject to change without notice.
MT-75
Tall gantry micromanipulator stand
$ 730
MT-75S*
Short gantry micromanipulator stand
$ 730
MT-75/LS
Tall gantry with linear slide
$ 1,295
MT-75S/LS*
Short gantry with linear slide
$ 1,295
MT-7004
Linear slide cantilever (no stand)
$ 700
*Useful for most upright scopes and the Axiovert 25 inverted scope with
low stage plate.
A quick lock mechanism allows easy
unlocking and rotation of the post/
cantilever assembly up to 320 degrees, then rotation back to the same
positive stop position and locking in
Vertical positioning is achieved with place (e.g., insertion and removal of
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
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2006_catalog.indd 72-73
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:17 PM
MICROMANIPULATION
75
MECHANICAL DRAWINGS MT-75
MECHANICAL DRAWINGS MT-75S
$//0($685(0(176$5(,1,1&+(6
MICROMANIPULATION
74
$//0($6(0(176$5(,1,1&+(6
$//0($685(0(176$5(,1,1&+(6
$//0($685(0(176$5(,1,1&+(6
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
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2006_catalog.indd 74-75
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:18 PM
MICROMANIPULATION
77
US PRICES
> MD-SERIES
MD SERIES
M I C R O M A N I P U L AT O R P L AT F O R M S
Series Microscope Model
International prices are 5% higher.
Prices subject to change without notice.
Part Number
Price
The last digit of the MD part number identifies a single sided platform (-1), or a double sided platform (-2).
MICROMANIPULATION
76
Nikon
MD-50 series
Nikon Diaphot 200/300
and Nikon TE 200/300
MD-50-1
MD-50-2
$ 456
$ 912
MD-51-1
MD-51-2
$ 456
$ 912
MD-52-1/UP
MD-52-2/UP
$ 456
$ 912
MD-53-1
MD-53-2
$ 456
$ 912
MD-60-1/L
MD-60-1/R
MD-60-2
$ 456
$ 456
$ 912
MD-61-1
MD-61-2
$ 456
$ 912
MD-80-1
MD-80-2
$ 456
$ 912
MD-81-1
MD-81-2
$ 456
$ 912
MD-90-1
MD-90-2
$ 456
$ 912
Axiovert 200
MD-91-1
MD-91-2
$ 456
$ 912
Axiovert 25
MD-92-1
MD-92-2
$ 456
$ 912
Nikon TMD
Nikon TE-2000
with Stage UP
Nikon TS-100
Leica
MD-60 series
Leica DMIRB*
For those who wish to use the
MP-285 or MP-225 manipulator
in conjunction with an inverted
microscope, Sutter Instrument has
designed a series of Microscope
Dependent (MD) stands. The typical
MD stand is a manipulator platform
that bolts directly to the frame of
an inverted microscope. Stands are
available in either single-sided or
double-sided versions for the most
common inverted microscopes made
by Nikon, Olympus, Leica, and Zeiss.
These platforms lend themselves to
the absolutely drift-free recording
configuration required when using
the manipulators to position patch-
electrodes to record from attached
cells in culture. To mount the MP285 to an MD stand you also need
either the 285210 mounting adapter
plate (for orthogonal mounting) or
the 285RBI rotating base that allows
for non-orthogonal mounting and
rotation of the entire manipulator
(note: the 285210 mounting plate
is included with each MP-285 and
MP-225). New mounting options
and adapters for new microscopes
are being added frequently. Please
see our web site for new additions,
current mechanical drawings, and
dimensions.
Leica DMIL
Olympus
MD-80 series
Olympus IX 50/70
Olympus IX 51/71/81
Zeiss
MD-90 series
Axiovert 100/135
* Please phone Sutter prior to ordering.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
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2006_catalog.indd 76-77
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
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MICROMANIPULATION
79
MECHANICAL DRAWINGS MD-SERIES
MICROMANIPULATION
78
)5217
$//0($685(0(176$5(,1,1&+(6
6,'(
3/$7)250
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
EM AIL: [email protected] |
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2006_catalog.indd 78-79
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
10/3/05 5:07:20 PM
MICROINJECTION
When Sutter Instrument set out to with air, oil or water, and that was
develop a brand new microinjection simple to dismantle and troubleshoot,
system, we were presented with a and,
very specific set of challenges:
4. To be able to offer these products
1. To create a micromanipulator with at a competitive price while maintainhighly-responsive movement that ing the quality expected of a Sutter
was intuitive and simple to use, yet product.
complete with time- and labor-saving
Using our 30 years of experience and
features,
expertise in motorized microposition2. To devise a pneumatic microin- ing, air pressure control and electrojector with an integral compressor mechanical design, we have created
capable of injecting at high pressures, the XenoWorks™ Microinjection
but also of gentle suction for holding System.
and transfer operations,
The new XenoWorks Microinjection
3. To offer a syringe-based microm- System has been designed to meet
eter-style microinjector that was the needs of a wide variety of appliextremely responsive, could be used cations for the manipulation of cells
2006_catalog.indd 120-121
and embryonic tissues. By combining
micromanipulators, microinjectors
and microscope mounting adapters,
systems can be configured for a wide
variety of applications including:
- Zygote pronuclear microinjection
- Embryonic stem cell blastocyst
transfer
- Nuclear and cytoplasmic transfer
- Piezo impact microinjection
- Intracytoplasmic sperm injection
- Adherent cell microinjection
traditional microinjection systems,
whether mechanical, hydraulic or
electronic, to be able to begin using
XenoWorks with as little learning
time as possible. It is, in a word,
intuitive.
On the next page are listed some of
the more common applications for
which XenoWorks is ideal. Please
contact us to discuss your specific
needs.
Real, tactile keys have been used
rather than membrane switches,
rotary controls turn smoothly and
responsively, and high-visibility LED
displays are used. Our intent is for
an investigator already familiar with
10/3/05 5:07:47 PM
MICROINJECTION
123
The configuration of any
microinjection system will depend
upon the application for which it is
used. Some common applications and
system configurations are:
Zygote pronuclear DNA
microinjection
The microinjection of DNA into the
pronucleus of a newly-fertilized
mammalian egg is now a common and highly efficient method
of creating transgenic offspring.
Pronuclear microinjection was first
described in the mouse, but now
many different transgenic animals
have been created in this way. Because the micropipette used for injection has an internal diameter typically
less than one micron, relatively high
pressure (>3000 hPa) is required to
inject the DNA solution. Two micromanipulators are required, one to
hold the zygote and one to inject
the DNA. Gentle negative pressure
is used on the holding side, while
pulses of high pressure are used to
inject 1–2 picoliters of DNA solution
into the pronucleus. The XenoWorks
Digital microinjector is ideal for
this application, having simultaneous
holding and high-pressure injecting
capabilities. Suggested system configuration:
1 x Micromanipulator (Right) BRMR
1 x Micromanipulator (Left) BRML
1 x Digital Microinjector BRE110/
BRE220
2 x Microscope Adapter
Embryonic Stem Cell Transfer
into Blastocysts
Animals, usually mice, can be engineered with a specific gene function
reduced or knocked out altogether
by introducing genetically altered
embryonic stem cells into the cavity of a blastocyst so that the stem
cells contribute to the embryo. The
resulting live animal is a chimera of
both genotypes. Subsequent selective interbreeding of manipulated
animals results in pure-bred gene
“knock-outs” or “knock-downs” and
can be used for subsequent gene
function studies. This operation requires two micromanipulators, one
for holding the blastocyst and one
for transferring the cells. Both holding and transfer functions require
gentle positive and negative pressure
for which the Digital Microinjector
is ideal. Alternatively, two Analog
Microinjectors may be substituted.
Suggested system configuration:
1 x Micromanipulator (Right) BRMR
1 x Micromanipulator (Left) BRML
1 x Digital Microinjector BRE110/
BRE220 or
2 x Analog Microinjector BRI
2 x Microscope Adapter
Intracytoplasmic Sperm
Injection
Intracytoplasmic sperm injection
(ICSI) can be employed for veterinary
in-vitro fertilization during rare species preservation or for any veterinary
assisted conception. ICSI may also be
used as a gene transfer technique
when sperm are co-injected with exogenous DNA (see also Piezo-assisted
ICSI, below). Typically, two micromanipulators are used, one for oocyte
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
INTL PHONE : 1- 415- 883- 0128 | INTL FA X : 1- 415- 883- 0572
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WEB: WW W .S U TTER .C OM
2006_catalog.indd 122-123
holding and one for sperm aspiration
and injection. Each micromanipulator
grips a single micropipette holder
with a microinjector attached. Since
low positive and negative pressures
are required for the delicate task
of oocyte holding and sperm injection, an ICSI workstation should
be configured with two Analog
Microinjectors. Suggested system
configuration:
1 x Micromanipulator (Right) BRMR
1 x Micromanipulator (Left) BRML
2 x Analog Microinjector BRI
2 x Microscope Adapter
of mercury inside injecting micropipette. Please note that mercury
should not be used in conjunction
with the Digital Microinjector,
though the Analog Microinjector
is ideal for this purpose. Suggested
system configuration:
MICROINJECTION
122
1 x Micromanipulator (Right) BRMR
1 x Micromanipulator (Left) BRML
2 x Analog Microinjector BRI
2 x Microscope Adapter
Microinjection of Cultured,
Adherent Cells
Cultured cell lines such as 3T3, CHO
and HeLa can be microinjected while
attached to a Petri dish. The procedure is best viewed through phasecontrast optics; a single micromanipulator and a single high-pressure
microinjection channel are required.
The tip of a sharp (inner diameter
less than 1 micron) micropipette is
brought down on top of a single
cell and a pulse of high (100–1000
hPa) pressure applied. The cell membrane is ruptured and the cell can
be seen to inflate slightly. Volumes
injected are typically less than 5%
of the cell volume. Success rates vary
widely depending upon the type and
volume of compound injected, the
culture conditions and the cell line
used. The high-pressure function of
the Digital Microinjector and the
smooth, fine control of the micromanipulator are particularly well-suited
to this application. Suggested system
configuration:
Piezo-assisted ICSI
This relatively new technique can be
employed for assisted conception
in animals in which standard ICSI
fails, such as mice. Piezo-assisted
microinjection has also been employed as a gene transfer method,
where sperm are coated in exogenous
DNA and injected into oocytes. The
microinjection workstation required
for this technique is very similar to
standard ICSI, but with the addition
of a piezo impact drive attached to
the injecting micropipette holder. The
device vibrates the injecting micropipette axially and drills its way into the
oocyte. This method has been shown
to increase success rates. Because the
micropipette is vibrating at minute
amplitude but high frequency, it is vital to use a mechanically stable micromanipulator, which will not vibrate
in sympathy. The more stable the
micromanipulator, the more efficient
the energy transfer from the piezo 1 x Micromanipulator (Right) BRMR
impact drive to the micropipette tip. 1 x Digital Microinjector
BRE110/BRE220
Some piezo-assisted microinjection
protocols currently require a bead 1 x Microscope Adapter
P H O N E : 1 -8 8 8 -8 8 3 -0 1 2 8 | F A X : 1 -8 8 8 -8 8 3 -0 9 0 0
I N T L P H O N E : 1 -4 1 5 -8 8 3 -0 1 2 8 | I N T L F A X : 1 -4 1 5 -8 8 3 -0 5 7 2
E M A I L : I N F O @ S U T T E R. CO M |
W E B: W W W . S U T T E R. CO M
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MICROINJECTION
125
Somatic Cell Nuclear Transfer
The enucleation of an oocyte followed by the transplantation of a
somatic cell is a method of producing
genetically identical copies (clones)
of the animal from which the donor cell was taken. Generally, two
micromanipulators are required,
one for holding the oocyte and one
for the enucleating and injecting
procedures. Each micromanipulator
grips a single micropipette holder
with a microinjector attached. Oocyte
holding, enucleation and somatic cell
transplantation require gentle, low
positive and negative pressure, making the Analog Microinjector ideal.
Suggested system configuration:
MICROINJECTION
124
1 x Micromanipulator (Right) BRMR
1 x Micromanipulator (Left) BRML
2 x Analog Microinjector BRI
2 x Microscope Adapter
Please contact Sutter Instrument for
help and advice with these and any
other microinjection needs.
The enucleation of an oocyte followed by the transplantation of a
somatic cell is a method of producing
genetically identical copies (clones)
of the animal from which the donor
cell was taken. Generally, two micromanipulators are required, one
for holding the oocyte and one for
the enucleating and injecting procedures. Each micromanipulator grips
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
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2006_catalog.indd 124-125
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MICROINJECTION
127
projecting into the microscope’s optical path, reducing the time taken to
find the pipette tip down the microscope. This also ensures that there is
adequate travel range in all axes of
motion prior to starting work.
XENOWORKS
M I C R O M A N I P U L AT O R
The manipulator uses a pre-programmed “Home” position for fast
micropipette exchange, and the ability to determine a “Work” position
which can be returned to with the
press of a button.
The manipulator requires no programming and next to no learning
time once set up and configured for
a particular microscope.
The XenoWorks Micromanipulator has been designed around our
hugely-successful MP-285 electrophysiology micromanipulator, with
the addition of a smooth-moving
adjustable inverted joystick. Because the motor drive is based on
an electrophysiology design, the
XenoWorks micromanipulator
is extremely stable and resistant to
ambient vibration. This stability
also makes this manipulator an ideal
platform for use in conjunction with
the PrimeTech PMM-150FU piezo
impact drive (see page 136), for
applications such as mouse nuclear
transfer and intracytoplasmic sperm
injection.
tion. The use of an inverted joystick
in conjunction with an almost zeroprofile base allows the operator to
rest their hands and forearms on the
bench surface, reducing the risk of
repetitive strain injuries. The function keys can be located and activated
by touch, obviating the need to look
away from the microscope, saving
time.
Another time saving feature is the
“Setup” function. Activating the
setup function places the micromanipulator in the optimum position
prior to beginning work (halfway on
the Y-axis, three-quarters down on
the Z-axis and three-quarters inwards
on the X-axis). Once the manipulaThe joystick offers an unprecedented tor is in the default setup position,
level of user comfort during opera- the micropipette tip will already be
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2006_catalog.indd 126-127
F E AT U R E S
> XENOWORKS
M I C R O M A N I P U L AT O R
Height and tension adjustable
inverted joystick for optimal
ergonomics
Exceptionally smooth and
responsive micropipette movement
Variable movement range –
6 settings from coarse to
ultra-fine
User-definable memory positions, including “Work” and
Z-axis lower limit
A wide 25mm of travel in
three axes
S P E C I F I C AT I O N S
> XENOWORKS
MICROMANIPULATOR
Travel
25mm on all three axes
MICROINJECTION
126
Maximum Resolution
62.5nm/microstep
Maximum Speed
5.5mm/sec
Range
X & Y Axis
Z Axis
(1 joystick swing) (1 rotation)
C
1
2
3
4
5
12,700 µm
3,300 µm
880 µm
400 µm
200 µm
100 µm
3,200 µm
800 µm
400 µm
200 µm
100 µm
50 µm
Joystick
Inverted joystick with integral
declutch
Height and resistance adjustable
Independent axis-polarity
inversion
6-position rotary range control
‘Setup’ function
1 user-defined set point (‘Work’),
1 user-defined axis limit
(‘Z-floor’),
2 preset points (‘Home’ and
‘Setup’)
Dimensions
Motor drive:
112mm x 185mm x 145mm
Joystick:
265mm x 240mm x 226mm
Control:
407mm x 280mm x 101mm
Electrical
115/230 Volts AC, 50/60Hertz
powerline
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MICROINJECTION
129
International prices are 5% higher.
US PRICES
> X E N O W O R K S M I C R O M A N I P U L AT O R
BRML
XenoWorks Micromanipulator (Left). Includes: 3 axis motor drive
(left-hand configuration), motor drive base plate, controller, joystick
user-interface, connecting cables, and manual.
$ 11,900
MICROINJECTION
128
BRMR
XenoWorks Micromanipulator (Right). Includes: 3 axis motor drive
(right-hand configuration), motor drive base plate, controller, joystick user-interface, connecting cables, and manual.
$ 11,900
XENOWORKS MICROSCOPE ADAPTERS
BR-L20 Leica DM-IRB/E/IRE2 adapter
BR-L30 Leica DM-IL adapter
$ 475
$ 475
BR-N10 Nikon TMD adapter
BR-N25 Nikon TE200/300 adapter
BR-N27 Nikon TE2000 adapter
$ 475
$ 475
$ 475
BR-Y50 Olympus IX50/70 adapter
BR-Y51 Olympus IX51/71/81 adapter
$ 475
$ 475
BR-Z30 Zeiss Axiovert 100/135 adapter
BR-Z35 Zeiss Axiovert 200 adapter
$ 475
$ 475
Prices subject to change without notice.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
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2006_catalog.indd 128-129
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MICROINJECTION
131
XENOWORKS
D I G I TA L M I C R O I N J E C T O R
Optimal ergonomics are again a
prime consideration; the remote
control unit provides access to all
keys and rotary controls necessary
to manipulate the parameters in the
two pressure channels, while the
separate compressor module can be
shelf- or floor-mounted away from
the microscope to eliminate vibration
interference.
S P E C I F I C AT I O N S
> XENOWORKS
MICROINJECTOR
Hold Function
Hold pressure:
-350 to +350 hPa
MICROINJECTION
130
Inject Function
Inject modes: Transient or timed
Inject pressure: 0 to 5600 hPa in
70 hPa increments
Injection time: 0 to 10 seconds
in 0.1sec increments
Inject activation: Transient;
hand or foot switch
Clear pressure: Ramps from
current Inject value to
5600 hPa at 1000 hPa/sec
Transfer Function
Transfer pressure:
-175 to +175 hPa
Maximum Pressure
5600 hPa (Inject channel)
Designed primarily for transgenic
mammal workstations, the XenoWorks Digital Microinjector is
capable of holding oocytes and early
embryos immobile while simultaneously providing a separate pressure
channel for pronuclear microinjection
of DNA or the gentle control of
embryonic stem cells as they are
transferred into blastocysts. The microinjector employs two independent
pressure control channels.
The self-contained vacuum channel
for gentle suspension-cell holding
applications can be switched on and
off as needed, and a responsive rotary
control is used to dial the pressure up
or down according to the degree of
hold required.
A second channel is available which
is capable of delivering both highpressure for solution injection, and
gentle positive/negative pressure for
transfer of embryonic stem cells. Two
modes of injection are available on
this channel; in Constant Flow mode,
the inject pressure set is applied to
the micropipette tip for as long as the
hand- or foot-switch is held down.
In Pulse mode, the inject pressure is
applied for a precisely timed duration, which can be set in 0.1 second
increments. Both injection duration
and pressure are easily selectable
using rotary controls on the remote
interface.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
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2006_catalog.indd 130-131
Controls
Tactile keys, rotary optical
encoders 3 x 7-segment LED
display
Tubing 2m ETFE
F E AT U R E S
> XENOWORKS
MICROINJECTOR
Micropipette Holder
Diameter: 4mm
Accepts 1mm capillary glass
(other sizes can be accommodated upon request)
Four injection modes:
Hold, Transfer, Inject, Clear
Electrical
BRE110: 100-120 Volts, 60Hertz
BRE220: 220-240 Volts, 50Hertz
Two independent pressure
channels
Rotary controls for continuous
pressure adjustment
Integral compressor with
remote control pad
Dimensions
Size:
Compressor module
407mm x 440mm x 150mm,
User interface module
164mm x 123mm x 70mm
Weight:
Compressor module: 1504g,
User interface module: 42g
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MICROINJECTION
133
International prices are 5% higher.
US PRICES
> X E N O W O R K S D I G I TA L M I C R O I N J E C T O R
BRE110
XenoWorks Digital Microinjector (110V). Includes: compressor module, user-interface module, 2 micropipette holders with 2m ETFE
tubing and fittings, spare tubing, connecting cables, and manual.
$ 6,900
MICROINJECTION
132
BRE220
XenoWorks Digital Microinjector (220V). Includes: compressor module, user-interface module, 2 micropipette holders with 2m ETFE tubing and fittings, spare tubing, connecting cables, and manual.
$ 6,900
BR-DT
XenoWorks digital tubing kit
$ 80
BR-MH
XenoWorks micropipette holder
$ 175
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
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2006_catalog.indd 132-133
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MICROINJECTION
135
XENOWORKS
ANALOG MICROINJECTOR
F E AT U R E S
> XENOWORKS ANALOG
MICROINJECTOR
S P E C I F I C AT I O N S
> XENOWORKS
MICROINJECTOR
Highly stable, drift-free
Coarse:Fine Ratio
10:1
Coaxial coarse and fine displacement (10:1 ratio) for fast
filling and precision control
Adjustable height control
Interchangeable syringes (500µl
standard, 100µl and 1000µl
options)
Effective with air, oil, and water
MICROINJECTION
134
Displacement/Turn
500µl syringe:
5.2µl coarse, 0.5µl fine
1000µl syringe:
10.4µl coarse, 1.04µl fine
Tubing
1m ETFE
Micropipette Holder
Diameter: 4mm
Accepts: 1mm capillary glass
(other sizes can be
accommodated upon request)
US PRICES
> XENOWORKS ANALOG MICROINJECTOR
The Xenoworks Analog Microinjector has been designed with ease
of troubleshooting in mind. While
fluid-filled hydraulic microinjectors
are the most responsive method of
manipulating cells and cellular components, over time they can develop
leaks and air bubbles which need to
be sealed or purged. The design of
the Analog Microinjector allows
the tubing to be easily separated
from the syringe and main injector
body and stripped to its components;
tubing and connectors. Parts such
as spare tubing are readily available
from Sutter (spare tubing and fittings are supplied with each injector)
and are quick and simple to fit using
commonly available Luer-lock and
ferrule connectors. Tubing length can
be altered according to users’ needs
and ancillary devices such as threeway valves may be fitted so that, for
example, a single injector can control
two micropipettes (for applications
such as blastomere biopsy).
BRI
XenoWorks Analog Microinjector. Includes: microinjector drive,
1 micropipette holder with tubing and fittings, spare tubing,
500 microliter syringe (100 or 1000 microliter syringe available
upon request).
$ 1,650
The Analog Microinjector comes
equipped with either a 100µl, 500µl
or 1000µl syringe as requested.
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2006_catalog.indd 134-135
BR-MH
XenoWorks micropipette holder
$ 175
BR-AT
XenoWorks analog tubing kit
$ 55
V001183
100µl gas-tight syringe
$ 110
V001185
500µl gas-tight syringe
$ 110
V001186
1000µl gas-tight syringe
$ 110
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MICROINJECTION
137
US PRICES
> PMM-150FU
PRIMETECH PMM-150FU
PIEZO IMPACT DRIVE
International prices are 5% higher.
Prices subject to change without notice.
PMM-150FU piezo impact drive.
Includes: Controller, operation box, piezo impact drive unit,
micropipette holder, tubing and pressure fittings, XenoWorks
Micromanipulator adapter
$ 13,600
BR-BXW
Replacement XenoWorks micromanipulator adapter*
$ 250
V010200
PrimeTech injection holder IH-3
$ 25
V010210
Spacer set for IH-3, Includes steel collar,
10 x 6mm silicon seal
$ 25
V200240
Tubing set for IH-3, Includes 1m Tefl
on tube,
PTFE
2 x Luer connector
$ 25
MICROINJECTION
136
*Adapters for other brands of micromanipulator are also available.
Sutter Instrument offers the PrimeTech PMM-150FU piezo impact drive
for challenging microinjection applications such as mouse nuclear transfer, intracytoplasmic sperm injection
(ICSI) and blastocyst injection. Oocyte
plasma membranes, particularly
those of the mouse, are particularly
sensitive to microinjection, which
tends to result in oocyte death. Initially designed for porcine oocyte
injection, the PrimeTech PMM was
adapted for use in the mouse in 1995
(Kimura and Yanagimachi, 1995),
and since then has become the gold
standard for mouse nuclear transfer
and ICSI.
The PMM-150FU harnesses the
“piezoelectric effect”: the tiny, but
extremely rapid distortion of a
piezoelectric crystal lattice when
an electrical current is applied to it.
This causes the micropipette holder,
which is placed in direct contact
with the piezo element to vibrate.
Two modes of piezo “drilling” are
available with the PMM-150FU: A
continuous train of pulses of desired
frequency and amplitude are used
to vibrate the micropipette, gently
penetrating the zona pellucida of an
oocyte or embryo, then a single pulse,
again of desired amplitude, is used
to break the oolemma and inject the
oocyte. This approach makes it pos-
sible to achieve up to 100% survival
of injected oocytes.
Included
XenoWorks Micromanipulator-specific micropipette holder clamp
PrimeTech factory warranty
Full technical support for piezo
impact applications
Kimura, Y. and R. Yanagimachi, 1995. Intracytoplasmic sperm injection in the mouse.
Biol. Reprod. 52, 709-720
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CUSTOM PRODUCTS
139
SAMPLE OEM PROJECT
OEM PRODUCTS
Customization of our optical product line for unique applications
has become a specialty for Sutter
Instrument. Our production facilities
include in-house electronic design,
circuit board layout, embedded
systems programming, and state-ofthe-art CNC machining and turning
centers for volume production of
precision mechanical components.
This expertise gives Sutter the ability to provide timely, cost-effective
engineering and manufacturing
solutions for Original Equipment
Manufacturers (OEMs). (The proven
performance and reliability of our
optical filter wheel systems has made
them a desirable choice for a range
of OEM applications. Examples of
custom designs include 5 position
and 10 position wheels with 2 inch
filters, 12 position arrays, compact
belt-driven filterwheels, cryogenic
and high vacuum filterwheels, rotating polarizers, an RS-485 serial bus allowing 16 controllers to share a single
serial port on a host computer, and
mounting adapters for a wide range
of microscopes. Sutter currently has
several significant OEM contracts and
will continue to establish new OEM
relationships. Please for more information about custom filter changing
devices.
PHONE: 1- 888- 883- 0128 | FAX: 1- 888- 883- 0900
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CUSTOM PRODUCTS
138
(A sample design project shown above: A cryogenic
dual wheel with twin adjustable rotating polarizers
for use in infrared astronomy)
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