BIO-Ångström 2008
Sensitive and high throughput multiplexed
immunoassays for biomarker discovery in
biobanked samples using proximity ligation assays
Simon Fredriksson, PhD
Chief Scientific Officer
Olink Bioscience, Uppsala Sweden
Why early detection of cancer?
Graph copied from: www.canaryfoundation.org
Diagnostic challenge in early cancer detection (screening)
- detection specificity (low false positive rate)
- detection senstivity (low false negative rate)
Solution...
•- the use of multiple biomarkers combined in a panel can improve
diagnostic accuracy
Research challenge in finding more biomarkers and biomarker panels
•- Limited supply of precious biobanked samples
•- Especially with repeat analyses
•- Large number of candidate biomarkers
•- Tens or even hundreds
Solution...
•- high throughput and multiplexed protein detection tools.
in solution PROXIMITY LIGATION
Multiplexed detection of proteins in plasma & serum samples
for biomarker research
Multiplexed in solution PLA
target proteins are converted into nucleic acid sequences
detected by real-time PCR
Fredriksson et al Nature Methods 2007
Features of PLA
-high senstivity
- low sample consumption
- wide dynamic range
in solution PLA
RnD systems ELISA
Multiplexed Proximity Ligation Assays
Pilot biomarker study in ovarian cancer
Samples from the POCRC
Clinical Chemistry 2008
PROACTIVE
Project coordinator
Olink Bioscience (Simon Fredriksson, Björn Ekström, Bonnie Tran, Erika Assarsson)
Assay development and reagents
Olink Bioscience and Innova Biosciences ltd. (Nick Gee)
Statistics and data management
Uppsala University dept of Medical Sciences (Mats Gustafsson)
Integromics S.L. (Marco Rodriguez)
Diagnostics expertise
Fujirebio Diagnostics (Olle Nilsson)
Samples and clinical expertise
Copenhagen University (Nils Brünner)
Awknowledgements
Uppsala University
Ulf Landegren's group at Rudbeck Laboratory
Stanford University
Ron Davis, Albert Koong, Rob Tibshirani