RIPARIAN PRAIRIE RESTORATION AND INTRODUCTION OF THE EASTERN SPADEFOOT TOAD April 24, 2014 A riparian restoration and introduction of an endangered amphibian species is proposed in detail at a site managed by the Athens Conservancy in Athens County, Ohio. (Photo credit: ODNR, 2012) Caleb Hawkins, MS Candidate in Environmental Studies;and Bill Rucker, MS Candidate, Department of Environmental and Plant Biology, Ohio University;Athens, OH 45701 For: Restoration Ecology with Dr. Jared DeForest Table of Contents Introduction ................................................................................................................................................. 2 Site Description ......................................................................................................................................... 4 Management Plan ....................................................................................................................................... 5 Measures of Success .................................................................................................................................. 13 Forecasting................................................................................................................................................. 14 References…………………………………………………………………………………………...……15 Timeline……………………………………………………………………………….......………………18 Budget……………………………………………………………………………………………….……20 Executive Summary: A 10 hectare riparian zone is restored to native tall grass prairie and two vernal ponds are constructed to introduce a breeding population of the endangered Eastern Spadefoot toad (Scaphiopus holbrooki). The restoration will commence on the Baker Easement (managed by the Athens Conservancy) southeast of Athens, OH in the spring of 2015, and will be done in collaboration with the OEPA, ODNR, and Ohio University. The objectives of this project are to: 1) introduce a breeding population of the Eastern Spadefoot toad to two constructed vernal pools and (2) increase biodiversity in a sand prairie riparian ecosystem. In order to achieve these goals, the restoration site is monitored to establish baseline amphibian and vegetative data, the site is plowed to prepare a seed bed, two vernal ponds (30 ft. x 40 ft.) are constructed with synthetic liners, prairie species are planted, and monitoring is performed to observe changes in species and community composition. The Eastern Spadefoot toad population is introduced from a known breeding site near Coolville, Ohio after heavy rainfall events occur. A portion of the reference population (in Coolville) is used to start a breeding population on the Baker Easement. Tadpoles will be transported in buckets and released into vernal pools accordingly. This project takes place from spring 2015 to spring 2018, depending on when breeding occurs. This restoration and introduction has numerous benefits to local stakeholders and the scientific community. The total cost of the project is $13,380.25. 1 Introduction: Riparian zones integrate terrestrial and aquatic ecosystems and contain a diverse array of landforms, communities, and microhabitats (Gregory et al., 1991). These dynamic ecosystems provide heterogeneous habitats dominated by high energy flood regimes for a wide diversity of plants and wildlife (Naiman, 2010). Riparian zones provide a variety of ecosystem services that include: relief of downstream flooding, restoring groundwater aquifers, promoting high primary and secondary productivity, exporting organic matter to streams, producing timber products, providing habitat and corridors for plants and animals, producing organic matter for aquatic food webs, increasing the capacity to recycle nutrients, providing buffer zones for water quality, sequestering metals and contaminants, and providing a sink for chemical compounds (Sharitz et al, 1992; Brinson et al. 1981). Moreover, riparian buffer zones of various widths and vegetation types are effective at removing dissolved nitrogen from surface waters (USEPA, 2007). Riparian zones throughout the world contain high levels of plant and animal diversity (Naiman, 2010). However, riparian ecosystems are threatened by a mosaic of factors that include: dams, pollution, grazing, land use change, timber harvesting, road construction, water diversion, recreation, mining, loss of native vegetation, fire suppression, beavers, insects, disease, salinity, invasive species, and climate change (Poff et al., 2011). Almost all riparian areas in the United States exhibit signs of degradation (Hoorman and McCutcheon, Ohio State University Extension, n.d.) and riparian areas in southeast Ohio are no different. Within these riparian areas is habitat that is essential to amphibian and reptile populations (Robeiro et al, 2012; British Columbia Ministry of Forests, 1998). The loss and fragmentation of has contributed to the endangerment of the Eastern Spadefoot toad (Scaphiopus holbrookii) (Conservation Commission of Missouri, 2014; Dr. Scott Moody, Pers. Comm. April 2014), which is Ohio’s only endangered toad (ODNR, 2012). Inhabiting alluvial soils of the Hocking River Valley, the Eastern Spadefoot toad is a rare and poorly understood amphibian native to the southeast and mid-Atlantic (University of Georgia, n.d.). The most recent available documentation of Spadefoot toad populations have been reported in 7 counties in Ohio, mostly in the southeast and central east part of the state: Athens, Meigs, Morgan, Washington, Scioto, Coshocton and Tuscarawas (Davis, 2013). Dr. Scott Moody, a professor in amphibian taxonomy and ecology at Ohio University, has indicated that there is a correlation between occurrences of Spadefoot toad populations with floodplain substrates of the Chagrin loam. The primary focus of this restoration proposal is to introduce a breeding population of the 2 Spadefoot toad into its inferred favorite ecological habitat on Chagrin loam soils. We will accomplish this by constructing two large (~90 m2) vernal ponds on a 5 ha. area that is part of the Baker Easement (Figure 1). The Baker Easement is a conservation and trail easement bequeathed for public use by the Baker sisters, daughters of Dr. John C. Baker, a past president of Ohio University. However, it is largely managed by the Athens Conservancy and this project will be done in collaboration with the organization. Fig. 1: Map of the Baker Easement just east of the city of Athens and Strouds Run State Park. Note that the wedge of land between SR 50 and the Hocking River is where the proposed project site will be located (map prepared by John Knouse from the Athens Conservancy). The secondary goal of this restoration is to restore part of the historic vegetation type surrounding the constructed ponds. Habitat fragmentation and the attendant loss of vegetation caused by prior land use activities has undoubtedly impacted biodiversity in the area. Nonnative plants have evidently established at this site particularly since it is located south of SR50, especially around the edges near the roads. But it is not just the plant communities that have been affected. For example, it is likely that insect, bird and small mammal populations that utilize the riparian zone along this stretch of the river have been negatively impacted. To remedy this, we will plow a rectangular, 5 ha. area to surround the constructed vernal ponds and plant a native prairie seed mix. The restored prairie will serve 4 purposes - it may: 1) provide improved habitat for the Eastern Spadefoot toad since that may have been the vegetative type in which the toad evolved; 2) promote further diversification of pollinators and other insects, birds and other small mammals; 3) provide a potential educational area or land lab for 3 local schools, universities, and watershed groups; and 4) increase the property bequest value for the Athens Conservancy. Site Description: This project focuses on a 5 hectare riparian zone by the Hocking River east of Athens, Ohio located in the Western Allegheny Plateau Ecoregion (Figure 2 and 3). The riparian zone on the north side of the Hocking River begins just northeast of the bridge on South Canaan Road (SR24A). In general, soils along broad floodplains such as the Hocking River are silty loams of fluvial and alluvial origin. The soil unit within the proposed site is the Chagrin loam, which is the first terrace above the normal floodplain, and it is rarely flooded (designated Cd by ODNR, 1985). The Chagrin loam is friable with moderate permeability, high water capacity, slow runoff and good tilth (ODNR, 1985). Figures 2 and 3 are scaled aerial photographs of the terrain and approximate site locations for the vernal ponds and restored prairie area. Figure 2: The area inside the box is a zoomed out aerial photo of the Baker Easement property. 4 Figure 3: The triangular wedge is the area within which the vernal ponds and surrounding restored prairie are located. Project Description: The objectives of this project are two-fold: 1) Introduce a breeding population of Eastern Spadefoot toad (Scaphiopus holbrookii) into two constructed vernal pools in a riparian zone; and 2) Encourage biodiversity by planting a tall grass sand prairie riparian zone surrounding the vernal pond sites. Management Plan: The prairie restoration and reintroduction project will be conducted in seven phases: (1) Survey the amphibian community at the reference site that we collect tadpoles from and a at the restoration site to see if there are any Spadefoot toads already, (2) seed bed preparation, (3) vernal pond construction, (4) plant native prairie seed, (5) confirm breeding population at reference site (6) introduce Eastern Spadefoot toad and (7) monitor and evaluate (Figure 4)1. 1 Monitoring and Evaluation is discussed in a separate section. 5 Survey of Reference and restoration site Seed bed preparation Spring 2014 Vernal pond construction Prarie seed planting Fall 2014 Sping 2015 Confirmation of breeding population at reference site Springsummer (2016-2018) Spadefoot Introduction at Restoration Site Springsummer (2016-2018) Monitoring and Evaluation (post Eastern spadefoot introduction) Figure 4: Project timeline Phase I: Surveys of Amphibian populations2 In order to determine baseline Amphibian community data at the restoration site and the reference Eastern Spadefoot population site, passive trap monitoring techniques will be employed during one-month period before seedbed preparation. Passive traps are very effective at determining distributions and abundances of amphibians temporally and spatially (Dodd, 2010). In some cases, drift fences are used to intercept breeding population as they migrate from terrestrial habitats to aquatic breeding locations (Dodd, 2010). As this would disturb breeding populations at both the reference site and restoration, this method will not be employed. Instead, the drift fences will be deployed throughout the riparian zone in terrestrial habitats (Figure 5). Deploying the drift nets and passive traps will enable project managers to compare amphibian abundance between the reference site and the restoration site (Dodd, 2010) and possibly to test other hypotheses about Spadefoot toad ecology, physiology, or any other testable feature of the site. Drift fences will be used in conjunction with pitfall traps that can be constructed from large plastic buckets (Figure 6). Drift fences and pitfall traps will be comparable between sites in order to eliminate sampling bias. We will employ drift fences and bucket traps at both sites for 1-month in order to establish baseline data and measure success. Post-restoration monitoring will also occur for one month in the spring for three years. The traps will need to be monitored daily and some water should be kept in the buckets in order to limit mortality in the traps (Dodd, 2010). Traps in both locations will be distributed in spatial arrays that are appropriate to the geographic area and representative of habitat. 2 Full schedule of activities shown in Table 2 6 Figure 5: Example of drift fence array and placement in two types of habitat (Dodd, 2012). This example shows 12 pitfall traps with 8 funnel traps. We will only use pitfall traps because they are effective and less expensive than funnel traps. Figure 6: Basic example of pitfall and drift fence design (http://www.yoal.net/cv/herp/imagens/figura4.jpg) Phase II: Seed Bed Preparation The proposed site on the Baker Easement is flat and only has small trees of the genus Prunus, Pyrus, and the native persimmon, Diospyros virginiana. We will keep any existing trees there and it will be easy to situate the ponds in areas where these species are not present. When we disk the remainder of the field, we will steer around the trees (Figure 7) (See Appendix 1 for two site photos). 7 Figure 7: 5 hectare area around vernal pools that will be plowed We expect a heavy seed bank from existing vegetation. We will not be able to control the germination of this seed bank, many of which are likely to be non-native invasive species. One of the most abundant ones is a non-native species of Lespedeza, a bush clover (Dr. Phil Cantino, Pers. Comm. April 2014), without the use of herbicide or burning. However, because the Athens Conservancy is opposed to herbicide use and because of the possible difficulties of acquiring permits for prescribed burning, we are opting to cultivate the site to prepare the seedbed for planting with the native prairie seed mix. In addition to the benefits outlined in the introduction regarding restoring native vegetation, disking and planting with the native prairie mix will enable direct competition with the non-native invasive species and, consequently, reduce their spread. Phase III: Vernal pond construction Vernal pond construction will be performed following instruction from the USDA Forest Service guide entitled “A guide to creating vernal ponds” (2002). Proper permits will be obtained from the US Army Corps of Engineers, the Ohio Environmental Protection Area, and the Ohio Department of Natural Resources. The ponds will be constructed using a synthetic liner method and will have dimensions of 30 ft. x 40 ft. The synthetic liner method was chosen because the substrate at the site is too permeable to hold water for sufficient amounts of time to create vernal pools. A dozer will be used to remove topsoil and make a depression in the soil that is no more than 2 ft. deep. Geotextile fabric will be placed above and below the synthetic liner in order to protect the liner from being punctured. After the liner is secured with stakes, a dozer will be used to cover the liner with soil. Within two-years the vernal ponds will look like natural features of 8 the landscape (Figure 8). Figure 8: Example of possible vernal pond sites and drift fence array placement. Phase IV: Planting of prairie seed mix The strategy to develop the planting plan was to first compile a list of native indicator species for sandy loam prairies in southeast Ohio. Fortunately, there is a blog post by an Athens County naturalist and professor, who has identified 10’s of species of grasses and wildflowers from the area that are moderately dry sandy soils. The web site can be explored at https://www.blogger.com/profile/07482657904308866348) Referencing this list, a mix was chosen that had as many species in common with this recommended list as possible. The seed mix also was recommended for relatively dry, sandy soils and near areas that are prone to disturbance, like this site is – both infrequent but intermittent flooding and weed seed drifting into the site by road traffic. Phase V: Confirmation of breeding at reference site During the spring of 2015, 2016, and/or 2017, a reference site where a known population of Eastern Spadefoot toad exists near Coolville, Ohio will be monitored after heavy rainfall events to see if breeding is taking place. If breeding is confirmed, then the sixth phase of the project will be initiated. 9 Phase VI: Eastern Spadefoot Introduction There are two important characteristics of the Eastern Spadefoot toad lifecycle that we must take into consideration to implement this project successfully. First, Spadefoot toad is an ‘explosive breeder’ and breeding behavior is triggered by both a drop in barometric pressure and heavy rainfall that creates temporary pools of water (pools need to last at least 3-4 weeks until fertilization, hatching, tadpole stage and metamorphosis into the adult toads occurs) (Davis, 2013 and Dr. Scott Moody, Pers. Comm. April 2014). It is for this reason that we included a prolonged, indefinite phase during which monitoring of the reference site will take place. Considering that the Spadefoot toad breeds sporadically depending on rainfall events, a threeyear time frame has been determined to confirm breeding at the reference site. If a sufficient population of tadpoles is found, then 10,000-20,000 tadpoles will be captured, transported in 5gallon buckets, and then introduced to the constructed ponds. Eastern Spadefoot has a Type I survivorship curve (Moody, Pers. Com.), therefore, of the population taken from the reference only about 2% are expected to reach maturity. The mature frogs will then establish themselves in the terrestrial habitat surrounding the vernal ponds, where they will dig backwards into relatively loose, friable sandy loam soils with their ‘spade’ foot and thickened webbing between their hind leg toes (Figures 9 and 10 from Davis, 2013). It is postulated that this is how they are able to maintain stable populations in floodplains during storm events (Moody, Pers. Comm. April 2014). Therefore, intermittent flooding should not disrupt the existing population. 10 Table 1: Species list for prairie planting Grasses Andropogon gerardii Big Blue Stem Schizochyrium scoparium Little Blue Stem Sorghastrum nutans Indian Grass Panicum virgatum Switchgrass Wildflowers Echinaceae purpurea Purple Coneflower Ratibinia pinnata Gray-headed Coneflower Asclepias hirtella Prairie Milkweed Asclepias tuberosa Butterfly Milkweed Liatris spicata Spiked Blazing Star Eurphorbia corollata Flowering Spurge Baptisia australis False Indigo Baptisia alba White False Indigo Lobelia spicata Spiked Lobelia Allium cernuum Nodding Wild Onion Delphinium carolinianum Tall Larkspur Delphinium tricomer Dwarf Larkspur 11 Figure 9: Spadefoot toad is named for the metatarsal ‘spade’ at the base of the first toe. Also note the thick webbing between the toes (Davis, 2013). Figure 10: Spadefoot Toad burrowing backward into the soil (Davis, 2013). 12 Measures of Success: Establishing Baseline Amphibian Data In order to establish baseline data for the Eastern Spadefoot toad populations for the project, pitfall traps will be installed for passive capture of reptiles and amphibians in the riparian zone where vernal pools have been installed in 2014. Within the reintroduction area auditory monitoring systems will also be installed. Pitfall traps, when used in conjunction with drift fences, is identified as the most effective method to capture many species (Anderson, 2011; Willson and Gibbons, 2010). These traps will allow field staff to determine species richness and abundance of amphibian biodiversity (and Eastern Spadefoot Toad populations) at the site prior to, and after, restoration is completed. Four drift fences will be constructed with 8 traps for each fence. A total of 32 pitfall traps will be used. Two drift fences will be located around the breeding ponds and two drift fences will be located at habitat transition zones. Species diversity indices will be calculated using the standard formulas for both the Simpson’s Index and the Shannon-Wiener Index. Restoration will be considered a success if the indices are higher post restoration. In order to measure project success, monitoring and on-site field investigation will take place annually for 3 years following the restoration and translocation project. This project will be considered a success if the following criteria are met: 1. Abundance and species richness of anuran communities is observed at the restored site every year for three years. This will be done by comparing baseline initial site data to restored site data. If the richness of Eastern Spadefoot toad increases by 30%, then the project will be considered a success. Furthermore, an increase of 2 or more in richness of other anuran communities will be an indicator of improved amphibian habitat within the riparian zone. Drift fences, pitfall traps, and funnel traps will be used to assess species that passively fall into the traps. The project will be considered a success if 300 adult Eastern Spadefoot are found at the restoration site per year. 2. A 25% observed increase in vegetation cover around the vernal pools. An automated recording system (ARS) will be used to assess amphibian populations as well. A Song Meter (model SM3, Wildlife Acoustics) will be used in the spring, summer, and fall to evaluate frog and toad calls during a three-year period. The Song Meter will be installed by a technician at randomly generated sites within the site from May 2015 to May 2016. Data will be collected 5 nights a week (excluding holidays). Field parameters such as temperature and precipitation will also be measured. Vegetative Assessments In addition to amphibian monitoring, we will measure the success of our vegetative planting in the tall grass prairie plantings.. For the tall grass prairie, we will measure % cover for each species that is identified. This will be done by setting up 3 parallel transects within the tall grass prairie. We will use a random sampling plot design along the 50 m length, setting out a 1 m square frame every 10 m along the transect; using a coin toss to randomize which side of the transect line the sample plot lays on. We will identify all species and quantify abundance in 4 13 ways: percent frequency in all quadrats (the sum of quadrats a species was in), relative frequency (% frequency for species a / sum of all species), % cover in all quadrats (sum of all covers for each quadrat it was in), relative cover (% cover for species a / sum of all species covers). From these sample plot datum, we will calculate relative importance values (RIV) calculated as one half the sum of relative frequency and relative cover for each species. In order to track annual changes in vegetation structure and composition for the field and vernal pool areas, a graph will be made for the ten most important species (by RIV) and the mean percent cover for those species plotted. Mean % cover is the total percent cover for each species divided by the total number of quadrats. The other graphical representation that we will use is to construct a dominance diversity curve (i.e. species abundance curve) for all species in the field by ranking each species along the ordinate according to its relative importance value (RIV). Forecasting: Vernal Ponds Vernal ponds will remain a fairly permanent feature since they are lined with ‘aquatic safe’ material. Although they will eventually deteriorate after some time, it is not critical that they remain indefinitely. The large excavated depressions will still remain for quite some time and this substrate is of the type that the Spadefoot toad normally inhabits. If we realize success with the introduction, then hopefully future generations of people will want to ensure that vernal ponds are maintained on the site. But even in the worst case scenario in which the introduction is not successful for unknown reasons, then the ponds would still be there for other anuran species. Prairie The site will be mowed every year after planting for the first three years in order to ensure reduced competition from non-native plants within the restored prairie habitat. The exception to this is that if we are able to introduce the toad within this 3-year window because of favorable breeding events at our reference location, then no mowing will be done because it might kill the toads. Due to the fact that we will not use herbicide treatments nor prescribed fire as an adjunct to mowing, and because the site is located close to a freeway (a major pathway for weedy seed sources) we do not expect complete replacement of existing vegetation with native prairie vegetation. However, native tall grass prairie species are deep rooting and, once established, they constitute very productive and vigorous ecosystems. 14 References Cited : Anderson, CJ. 2011. The role of riparian wetland restoration in amphibian conservation in the North Carolina Piedmont. Master’s Project. Duke University. http://dukespace.lib.duke.edu/dspace/bitstream/handle/10161/4653/AndersonCatherine_MP_Sept11.pdf?s equence=1 Beatty, E.G and Stone, M.S. Getting to Know Athens County. The Stone House. Athens, OH 1984. Biebighauser, TR. 2002. A Guide to Creating Vernal Ponds. USDA Forest Service. Brinson, M. M., B.L. Swift, R.C. Plantico, and J.S. Barclay, 1981. Riparian ecosystems: their ecology and status. FWS/OBS-81/17. United States Fish and Wildlife Service, Biological Services Program, Washington, D.C., USA. Davis, JG. 2013. Book Chapter “Eastern Spadefoot” in Amphibians of Ohio, eds. Pfingsten, R.A et al. Ohio Biological Survey. pp.685-707. Dodd, C. K. (2010). Amphibian ecology and conservation. A Handbook of Techniques (2010). Gregory, K., & Davis, R. (1992). Coarse woody debris in stream channels in relation to river channel management in woodland areas. Regulated Rivers: Research & Management, 7(2), 117-136. Gregory, S. V., Swanson, F. J., McKee, W. A., & Cummins, K. W. (1991). An ecosystem perspective of riparian zones. Bioscience, 41(8), 540-551. Gregory, Stanley V., et al. "An ecosystem perspective of riparian zones." BioScience 41(8), pp.540-551. Karr, J. R. (1981). Assessment of biotic integrity using fish communities. Fisheries, 6(6), 21-27. Lester, R. E., Wright, W., & Jones-Lennon, M. (2007). Does adding wood to agricultural streams enhance biodiversity? an experimental approach. Marine and Freshwater Research, 58(8), 687-698. Naiman, R. J. (2010) "Riparian Zones." River Ecosystem Ecology: A Global Perspective: 182. Ohio Environmental Protection Agency. 2006. Methods for Assessing Habitat in Flowing Waters: Using the Qualitative Habitat Evaluation Index (QHEI). Ohio EPA Division of Surface Water, Columbus, Ohio. 26 pp. http://www.epa.ohio.gov/portals/35/documents/QHEIManualJune2006.pdf Ohio Environmental Protection Agency (2009). Hocking River Watershed TMDL Report. Division of Surface Water, Columbus, OH. Pennsylvania Stormwater Best Management Practice. Chapter 6. http://www.elibrary.dep.state.pa.us Osborne, L. L., & Kovacic, D. A. (1993). Riparian vegetated buffer strips in water‐quality restoration and stream management. Freshwater Biology, 29(2), 243-258. 15 Ohio State University Extension http://ohioline.osu.edu/ls-fact/0001.html http://srelherp.uga.edu/anurans/scahol.htm Poff, B., Koestner, K. A., Neary, D. G., & Henderson, V. (2011). Threats to riparian ecosystems in western north america: An analysis of existing literature1. JAWRA Journal of the American Water Resources Association, 47(6), 1241-1254. Riparian Areas: Providing Landscape Habitat Diversity (1998). Extension Note. Ministry of Forest Research Program. British Columbia Ministry of forests. http://www.for.gov.bc.ca/hfd/pubs/docs/en/en17.pdf Ribeiro, J. W., Lima, A. P., & Magnusson, W. E. (2012). The effect of riparian zones on species diversity of frogs in amazonian forests. Copeia, 2012(3), 375-381. Sharitz, R. R., Boring, L. R., Lear, D. H. V., & Pinder III, J. E. (1992). Integrating ecological concepts with natural resource management of southern forests. Ecological Applications, 2(3), 226-237. Soman, S. et al. 2007. Ecosystem Services from Riparian Areas: A Brief Summary of the Literature. University of Illinois (unpublished draft). http://www2.illinois.gov/ltgov/documents/sac/sacriprarianservices.pdf Sweeney, B. W., Bott, T. L., Jackson, J. K., Kaplan, L. A., Newbold, J. D., Standley, L. J., et al. (2004). Riparian deforestation, stream narrowing, and loss of stream ecosystem services. Proceedings of the National Academy of Sciences of the United States of America, 101(39), 14132-14137. United States Environmental Protection Agency. 2007a. Riparian Zone and Stream Restoration. Assessed at. http://www.epa.gov/ada/eco/riparian.html United States Environmental Protection Agency. 2007b. Research shows importance of riparian buffers for aquatic health. Science in ACTION: Ecological Research Program. Assessed at http://www.epa.gov/nrmrl/pubs/riparianbuffer.pdf. Wilson, J.D. and J.W. Gibbons. 2010. Drift fences, coverboards, and other traps. In Dodd, C.K. 2010. Amphibian Ecology and Conservation: A Handbook of Techniques. New York: Oxford University Press. Available at: http://comp.uark.edu/~jwillson/publications/2009%20-%20Willson%20&%20Gibbons%20%20Amphibian%20traps%20-%20BOOK%20CHAPTER.pdf 16 Table 2: Timeline Timeline 2014 Activity JanApr MayAug 2015 SepDec JanApr MayAug 2016 SepDec JanApr Requisition of permits Preliminary surveys of restoration and reference sites Seed bed preparation Vernal pool construction Planting of prairie seed Eastern Spadefoot Introduction Monitoring End of Project 17 MayAug 2017 SepDec JanApr MayAug 2018 SepDec JanApr MayAug SepDec Budget: Total costs of the project amount to $13,380.25 (Figure 11 and Table 2). Budget Breakdown 12% 2% 28% Vernal Pond Construction Prarie Restoration Monitoring Spadefoot relocation 58% Figure 11: Budget breakdown. The majority of costs for this project involve the prairie planting and vernal pool construction. 18 Table 3: Itemized Budget Item Vernal Pond Construction Dozer Synthetic liner Geo-textile fabric wire stakes straw for mulch Unit Cost hours ft Quantity Total $78.81 10 $788.10 2 $0.50 2400 $1,200.00 2 $0.33 $0.33 $4.93 4800 100 30 $1,584.00 $33.00 $147.90 ft each bales Sub total Prairie Restoration Prairie seed mix Tilling hire Sand Hitch Seed spreader lbs hours 50-lb bag each $54.50 $80.00 $4.48 $278.00 $3,753.00 125 8 5 1 Sub total Spadefoot Relocation Small fish nets (16") gas for vehicle bucket (5 gallons) truck rental each each each day $3.67 $20.00 $12.00 $100.00 $7,752.90 5 1 15 1 Sub total Monitoring SM3 Acoustic Monitor Plastic for drift fences Buckets (5 gallons) Hula hoops each 2 ft each each $13,380.25 19 $18.35 $20.00 $180.00 $100.00 $318.35 $900.00 1 $900.00 $0.50 $12.00 $4.00 800 20 4 $400.00 $240.00 $16.00 Sub total TOTAL $6,812.50 $640.00 $22.40 $278.00 $1,556.00 Budget Justification Vernal Pond Construction: The construction of vernal ponds is absolutely integral to the success of the Spadefoot relocation portion of this project. Eastern Spadefoot toad is a prolific breeder that uses vernal ponds to spawn. Two vernal ponds will be constructed. The budget was calculated using “A guide to creating vernal pools” (USDA, 2002) and prices were adjusted for inflation. The dozer will be needed to create a depression for the vernal pools. The synthetic liner is needed in order to ensure that vernal ponds stay filled with water during wet periods. The geotextile fabric will protect the synthetic liner from being punctured. Wire stakes will be used to tack down the synthetic liner and the straw will be used to protect the newly sown seed from erosion around the ponds. Prairie restoration: The prairie restoration of the project is needed to restore biodiversity and habitat for the Eastern Spadefoot toad. Prairie seed mix will ensure that some native prairie plants establish after seeding. A till will be needed to prepare the seed beds for seeding. Sand will be used as a matrix to distribute seed. A spreader will enable seed to be sown easily and quickly. Spadefoot relocation: Fish nets and buckets are needed in order to capture and transport tadpoles to the restoration site. The truck rental and gas are needed in order to transport tadpoles. Monitoring: An SM3 acoustic monitor from Wildlife Acoustics will be used to vocal amphibian species after the restoration portion of the project is completed. Buckets and plastic are needed in order to create traps for amphibian sampling that will establish population counts at the restoration site. Hula hoops will be used to vegetation monitoring assessments. 20 Appendix 1: Photo 1 and 2: Two views of the proposed site standing approximately 100 meters from SR 50 and facing southeast and southwest, respectively, toward the Hocking River. Note that there are no large trees, it is nearly flat, and the vegetative cover is mostly herbaceous perennials. Both photos taken by Bill Rucker on 4/23/14. 21 Appendix 2: The map in this appendix was prepared by John Knouse, courtesy of the Athens Conservancy. It shows the extent of the Baker Tract. The triangular section adjacent to the Hocking River is the portion of the tract that we will restore. 22
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