Table of Contents 2013 Acknowledgements ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ 2 2012/2013 Steering Committee Members ~ ~ ~ ~ ~ 3 Keynote Speaker (9:00 am Centennial Hall Auditorium) ~ ~ ~ ~ ~ 6 Schedule of Events ~ back cover Student Presenter Abstracts ~ ~ ~ ~ ~ ~ ~ ~ ~ ~ 7-87 Oral Session I (10:00 – 11:00 am) ~ 7 Poster Session I (11:00 am – 12:30 pm) ~20 Oral Session II (1:00 – 2:00 pm) ~44 Poster Session II (2:00 – 3:30 pm) ~53 Oral Session III (3:00 – 4:00 pm) ~77 The Colorado Springs Undergraduate Research Forum (CSURF) is a collaborative venture designed to highlight the accomplishments of undergraduate students from Colorado College, the United States Air Force Academy, and the University of Colorado Colorado Springs. In its tenth year, the conference has grown to over 350 participants and continues to reflect the commitment and dedication of our faculty and staff to our students from all disciplines. Participation in the CSURF is open to currently enrolled undergraduates of CC, USAFA, and UCCS. Complete information on the CSURF may be found at www.uccs.edu/csurf . Thank You for your continued support of the Colorado Springs Undergraduate Research Forum University of Colorado Colorado Springs (2013 Host) Colorado College and United States Air Force Academy ~2~ ~ CSURF Steering Committee Members ~ University of Colorado Colorado Springs Matt Barton Assistant Professor of Sculpture Visual Art Program Co-Director Department of Visual and Performing Arts University of Colorado Colorado Springs Cory Drieth Co-Director/Assistant Professor of Visual Art Department of Visual and Performing Arts University of Colorado Colorado Springs Cerian Gibbes Assistant Professor Department of Geography and Environmental Studies University of Colorado Colorado Springs K. Alex Ilyasova Assistant Professor Department of English University of Colorado Colorado Springs Aditi Mitra Assistant Professor Department of Sociology University of Colorado Colorado Springs Margie Oldham Director of Community Relations College of Letters, Arts, and Sciences University of Colorado Colorado Springs Robert (Rex) Welshon Associate Dean Professor Department of Philosophy University of Colorado Colorado Springs Thomas Wolkow, Chair CSURF Committee Associate Professor Department of Biology University of Colorado Colorado Springs ~3~ Colorado College Pedro de Araujo Assistant Professor Department of Economics and Business Colorado College Murphy Brasuel Associate Professor Department of Chemistry and Biochemistry Colorado College Scott Krzych Assistant Professor Department of Drama/Dance Colorado College ~ United States Air Force Academy Dr. Devin Della-Rose Associate Professor Department of Physics United States Air Force Academy Julie Imada Research Publicist Office of Research United States Air Force Academy Colonel Robert Kraus, USAF Chief Scientist and Director of Research Office of Research United States Air Force Academy Erin Miller Research Programs Manager Office of Research United States Air Force Academy ~4~ Lieutenant Colonel Brian Paeth, USAF Assistant Professor Department of Physics United States Air Force Academy Charity Peak Assistant Professor Student Academic Affairs and Academy Registrar United States Air Force Academy Lieutenant Colonel Mario Serna, USAF Assistant Professor Department of Physics United States Air Force Academy ~5~ Keynote Speaker 9:00 am ~ Centennial Hall Auditorium Collective Creativity: A Model for Responsive Structures and Research Thomas Wilson The performing arts, and especially music, provide unique models of organizational structure. By understanding and adapting music’s unique modes of communication, we can break through the usual boundaries of hierarchies and linear research models to create more responsive systems that foster individual and collective creativity. ~6~ Oral Session I (10:00-11:20 am) Polymers, Air Drops, & Dorm Food Room: University Center 309 ~ SUPPLY CHAIN MAPPING AND INVENTORY MODELING FOR RMB PRODUCTS Dave Dunkel, Jeremy Fabling, Greg Kidd, Greg Renner with Lt Col Tim Pettit and Capt Brian Lemay (Advisors) Department of Management United States Air Force Academy RMB Products is a company that specializes in engineered polymers that meet the needs of business sectors such as aerospace, chemical processing, semiconductors, and biopharmaceuticals. RMB Products is growing and is looking to expand their supply-side analyses and management. This project is a unique inventory management analysis that provides RMB Products with two tools to help them better understand their supply chain and more efficiently stock inventory. The first tool is a supply chain map that displays suppliers and customers and then graphically represents multiple characteristics of each of these suppliers and customers, including characteristics of the relationships between them. The second tool is a simulation program that performs inventory analyses by modeling the effects of various inventory policies. In addition, this model allows simulation of operations under various conditions, including partial and complete failure of the supply chain. The goal of this project is to decrease average inventory by $500,000 by better balancing supply chain resilience. ~ ANALYSIS AND IMPROVEMENT OF THE JOINT PRECISION AIR DROP SYSTEM Joseph Abakunda and Cameron Igawa with Lt Col Tim Pettit (Advisor) Department of Management United States Air Force Academy Currently, precision air drops are too costly. With a desired end-state of 25-yard accuracy from drop altitudes of up to 25,000 feet, payload weights from 200 to 10,000 pounds for just under $5,000 per load, there are several improvements still needed ~7~ before the current Joint Precision Air Drop Systems (JPADS) can meet these requirements. An additional requirement is the elimination of the weather acquisition over-flight. This project, using a systems engineering mindset, examines the systems integrating into the Precision Air Drop solution and identifies different variables from multiple disciplines to integrate into the system. Using various US Air Force sources to collect and categorize existing data and identifying missing data, we intend to integrate systems requirements into modeling and development efforts. ~ MITCHELL HALL ANTHROPOMETRIC ANALYSIS Blake Abrecht with Capt James Walliser (Advisor) Department of Engineering United States Air Force Academy An often overlooked aspect of workplace safety and an underlying cause of employee injury is the design of the physical workspace. The research team sought to identify the ergonomic issues that may have been present in the United States Air Force Academy’s dining facility, Mitchell Hall. The project evaluated the appropriate amount of weight that Mitchell Hall workers can safely lift, suggested modifications to the organization of the dining facility, and introduced a compliance plan to ensure safe lifting standards. The National Institute for Occupational Safety and Health (NIOSH) lifting equation was utilized to determine recommended weight limits as well as lifting indexes for various tool/storage location combinations within Mitchell Hall. A virtual model of the dining facility was programmed with the JACK/JILL software suite in order to analyze collected data, recommend improvements to the workspace, and enhance occupational safety and health. Overall, this project found that Mitchell Hall adheres to the highest of safety standards; however, multiple recommended weight limits flagged concern regarding various current lifting and storage practices. While not egregious safety hazards, the storage of food trays and the loading of sauce into cooking pots have potential for safety hazards if not handled properly. This project supported previous workspace safety analyses that have been performed in Mitchell Hall and through redundancy and continual knowledge regarding workplace safety, ultimately increased workplace safety at the United States Air Force Academy. ~8~ _____________________ Human Factors I: Professor Calkin’s Class Room: Centennial Hall 106 ~ THE DIFFERENCES BETWEEN READING COMPREHENSION TEST SCORES USING DIFFERENT MEDIUMS – KINDLE, COMPUTER, PAPER Erin Doran, Holley Macpherson, Carlo Mancini, Tyler Vincent with Maj Bryan A. Calkin (Advisor) Department of Behavioral Sciences and Leadership United States Air Force Academy As the world becomes increasingly digitally-oriented USAFA students still frequently print out class reading materials so they do not have to read from a computer screen, or use a Kindle ® for their assignments. The research team asserts this is because it provides the student better reading comprehension. The present study will investigate whether this perceived preference in reading mediums manifests itself in reading comprehension differences between mediums. The mediums that will be tested are paper, Amazon Kindle ® and laptop computer. The study will also utilize measures in the form of a post-reading comprehension quiz and a post study survey. ~ THE EFFECTS OF CALCULATOR TYPE ON TEST PERFORMANCE Haley Holcombe, Marcela Leaño, Jack Miller, Joel Parritt with Major Bryan A. Calkin Department of Behavioral Sciences and Leadership United States Air Force Academy All students at the United States Air Force Academy are subject to a frustrating rule throughout freshmen and sophomore year that forces the use of only one calculator, the TI-30, in all math based core classes. While this seems like a standard simple rule, it has been the basis of grief for many students over the years. Many cadets enter the academy with a preference for the TI-84, a graphing calculator commonly seen in high schools across the country. Having to switch from a calculator with a large screen and familiar buttons to a small, cheap calculator can be very frustrating especially when the difficulty level of math has gone up. The present study will investigate performance and mental workload when students use both the TI-30 and the TI-84 to see if the type of calculator affects performance when test taking. The study will focus on a student’s ability to complete a timed arithmetic test with each of the two types of calculators. Completion rate and a survey of mental demand will be the determining factors. ~9~ ~ THE EFFECTS OF USING NAVIGATION AIDS ON A DRIVER’S VISUAL SCAN AND PERFORMANCE Stephen Katrein, William Peterson, Jared Rillings, Prittany White with Maj Bryan A. Calkin (Advisor) Department of Behavioral Sciences and Leadership United States Air Force Academy The present study investigates how different combinations of external stimuli affect a person’s ability to effectively operate a motor vehicle in a simulated driving task. More specifically, this study will examine how auditory and visual stimuli affect a driver’s performance. We hypothesize that directions delivered audibly will affect a driver’s performance less than directions that are delivered visually. In order to compare these delivery methods, several dependent variables will be analyzed. The conditions will include a baseline condition, with no auditory or visual aids, along with two experimental conditions, one with an auditory aid and one with a visual aid. These data will be analyzed to determine which combination of aids had the greatest effect on the driver’s visual scan pattern and ability to navigate through the driving simulation. _______________ Better Living through Biology Room: Centennial Hall 186 ~ THE ANALYSIS OF OBSERVED X-RAY CRYSTALLOGRAPHIC PROTEIN STRUCTURES Christopher Brandon with Dr. Sonja Braun-Sand (Advisor) University of Colorado Colorado Springs The ongoing research being performed aims to identify structural mistakes in published X-ray crystallographic structures of proteins in order to help generate more precise 2º, 3º, and 4º protein structures. The structural data is gathered from the Protein Data Bank and a commercial software package, MOPAC, is being used to find inconsistencies in atomic charge, bond length, atomic interactions, and other structural variations which would affect the proteins accepted 3-dimensional shape. Protein structure is integral to the function of that protein and with more accurate data we can better analyze how it functions. Once structural inconsistencies have been identified, the protein structure will be optimized to the lowest heat of formation (Hf) by MOPAC and that structure will be used as a starting point for calculating reaction barriers in the enzyme. ~ 10 ~ ~ THE EFFECT OF RALSTONIA PICKETII ON CORROSION FATIGUE IN AEROSPACE ALUMINUM ALLOYS Sarah E. Collins Department of Engineering Mechanics United States Air Force Academy The Center for Aircraft Structural Life Extension (CAStLE) at the USAF Academy has been investigating corrosion fatigue in these 7xxx series aluminum alloys. During corrosion fatigue testing of these alloys in 0.06 M NaCl, Ralstonia pickettii bacteria growth was noted which slowed the corrosion fatigue crack growth rate. The presence of R. pickettii in the salt water solution has been shown to decrease the fatigue crack growth rate from 3.4 x10-4 mm/cycle to as low as 9.0x 10-5 mm/cycle at a stress intensity (∆K) of 6 MPa√m, stress ratio (R) of 0.65 and loading frequency (f) of 0.05 Hz. The mechanism for how the bacteria are able to lower the fatigue crack growth rate is being investigated. Current research is investigating metal sequestration, desalination and conductivity effects of the bacteria to determine how the corrosion fatigue crack growth rate is being slowed. ~ INVESTIGATING THE TEMPERATURE STABILITY OF THE REAL-TIME PCR DYE EVAGREEN Jason W David with Melanie LM Grogger*, Daniel H Atchleyǂ, Donald V Veverka* (Advisors) *Department of Biology United States Air Force Academy ǂ College of Pharmacy Harding University Real-time PCR (qPCR) is a widely utilized molecular biology technique necessary to many modern research applications. Arguably, the most important reagent used in qPCR is the fluorescent dye. Virtually all intercalating qPCR dyes, including EvaGreen (Biotium, Hayward, CA), require cold storage. This limitation is a major factor in the versatility of qPCR, especially concerning field use. Previous testing of EvaGreen in our laboratory has shown that EvaGreen remains stable at storage temperatures of 25°C, 45°C and 65°C. Dye taken on deployment to the Middle East without cold storage has demonstrated similar stability. This research conducts a more robust stability analysis of EvaGreen. Dye samples were temperature stressed at 25°C, 45°C and 65°C and compared to controls stored at 4°C. These samples were used with GE Ready-to-Go beads (GE Healthcare, Pittsburgh, PA), over a series of time points to detect the InvA gene of Salmonella typhimurium. Investigation of reported Ct and fluorescence values was used to determine relative stability. These results strongly suggest that EvaGreen may be stable at much higher storage temperatures than recommended by the ~ 11 ~ manufacturer and when employed, may allow for qPCR use in many versatile applications, including deployment in the field without cold storage. _____________________ Telescopes, Criminals and AI Room: Centennial Hall 188 ~ RULES BASED PRIORITIZATION FOR THE FALCON TELESCOPE NETWORK Peter Jackson with Dr. Francis Chun (Advisor) Department of Physics United States Air Force Academy A common problem for the processing of tasks is to decide what task is run first while still attempting to make sure that everyone gets a turn. This is one problem that must be overcome in order to develop the U. S. Air Force Academy Falcon Telescope Network (FTN). The purpose of this project is to develop and implement a policy in order to weight or prioritize user observation task requests. These tasks contain a set of requirements that define an image or set of images to be taken by a telescope. Rules were developed by discussing the important observational criteria with experts and by examining the requirements that had to be fulfilled. Furthermore, we analyzed how well different prioritization methods worked first for a large sample size and then for a smaller, more realistic sample size. As a result of this work, a way to determine the weight or priority of a task was developed for use in the FTN. These rules are specific to the application of the FTN as each application has different priorities and priorities may change over time. ~ CRIMINAL BEHAVIOR ANALYSIS: IDENTIFYING REPEAT OFFENDERS Aaron Burns, Zebulon Hanley, Rebekah McKenna, and Zachary Smith with Lt Col Chris Solo and Capt Brian Lemay (Advisors) Departments of Mathematical Sciences and Management United States Air Force Academy As the idea of “Intelligence Led Policing” grows in popularity throughout the country, police departments are looking to utilize analytics to most effectively utilize their finite policing resources. Currently, the Colorado Springs Police Department (CSPD) has a simplistic Repeat Offender Model (ROM) that aims to identify criminals who are likely to commit additional crimes. This model helps prioritize policing activity and highlights individuals whose criminal behaviors might normally escape the attention of police. This ~ 12 ~ study addresses the limitations of CSPD’s current ROM and provides data-driven recommendations to improve the ROMs effectiveness. Specifically, this study provides CSPD with a more complex ROM that uses the types of offenses committed by an individual when determining the propensity of the person to commit additional crimes. Statistical analysis of historical criminal data and computer programming is used to automate model updates and provide a useable interface to CSPD. Model verification and validation is emphasized to ensure potential use of the ROM in criminal courts. ~ Jessica Gronski Department of Mathematics University of Colorado Colorado Springs An artificial neural network (ANN) is a mathematical/ computational model inspired by the structure and functional aspects of biological neural networks: it is an interconnected group of artificial neurons that finds the relationship of certain inputs and their corresponding outputs. Artificial Neural Networks are trained through a trial and error process in which the network learns how to reproduce the correct outputs from the given inputs by adjusting the statistical weights on the connections between different layers of neurons. For the specific problem, we will examine the relationship within the complex dynamics of a medical ward, given an ample amount of various input data sets (consisting of positive and negative events, number of medical staff present and admittance and discharge of patients in the ward) and their corresponding outputs (the ward atmosphere ranging from calm to chaotic). The goal of an ANN-based mathematical modeling approach is to produce a quantitative analysis of the system, which at the same time has a chance to be integrated with the more qualitative psychosocial information, thus gaining the attention of both fields as a useful tool of investigation. _____________________ Studies in Humanities Room: University Center 302 ~ FATE AND THE GODS AS ANALYZED THROUGH GREEK LITERATURE Irene Joyce University of Colorado Colorado Springs The purpose of this paper is to analyze the role of fate in the lives of the Ancient Greeks through its traditional foundation in the works of the poets Hesiod and Homer. The poets’ writings on fate describe its role in a perplexing manner, which leads to confusion among the Greek citizens and their concept of fate. Fate is portrayed as something ~ 13 ~ determined above the gods, and yet the gods have power to alter shortcomings in fate. The different roles fate plays in the lives of the Greeks influences how they strive to live well, appeasing the gods and dealing with imminent death. ~ JUSTICE WITHIN PROMETHEUS BOUND AND ANTIGONE AND THE PLATONIC ALTERNATIVE Kyle Singletary with Dr. Andrea Van Nort and Lt Col Rouven Steeves (Advisors) Department of English and Fine Arts United States Air Force Academy The conception of justice within Greek tragedy that “Dike will have her due” is keenly articulated within Aeschylus’ Prometheus and Sophocles’ Antigone. This paper will explore how the aforementioned conception of justice is displayed in these tragedies, and how Plato offers an alternative to the tragic fates of the characters in these works. Within Prometheus, we will see how Prometheus’ attempt to save man while disregarding the laws of the gods, namely the law of Zeus, causes justice to exact its toll on Prometheus. Interestingly, Zeus also suffers, in large part as a result of his hubris. Due to his own injustice done to Io, he is faced with the prospect of punishment in that his own son will depose him, just as he deposed his own father. The same retributive aspect of justice also applies to Antigone. She upholds the laws of the gods but disregards man’s law, which leads to her own demise. Yet as with Zeus, we see that the creator of the laws, Creon, also suffers due to his hubris. All four of these characters display an excessive amount of hubris and immoderation. In the final section of the paper, the Platonic conception of the soul will be discussed as a corrective to the hubris evinced in the plays. The conclusion will be that proper ordering of the soul, with moderation in action and temperament, leads to justice both in the individual soul and within the soul of the polis. ~ WOMEN’S ROLE IN THE MEXICAN REVOLUTION IN THE NOVEL LIKE WATER FOR CHOCOLATE Elizabeth Maldonado Department of Foreign Languages United States Air Force Academy Women’s participation in the Mexican Revolution (1910-1920) opened the doors for gradual change in Mexican women’s rights and social change. The novel, Like Water for Chocolate (1989), by contemporary Mexican novelist, Laura Esquivel (1950), demonstrates several parallels between the female characters in the domestic life during the Mexican Revolution and the real women who bravely participated in the Revolution. This is demonstrated through Esquivel’s extended metaphor of Mamá ~ 14 ~ Elena, the novel’s antagonist, and the Mexican dictator and primary cause of the Revolution, Porfirio Diaz (1830-1915). The relationship between the novel and the Mexican Revolution extends to a comparison between the Mexican people who rose in rebellion for freedom of oppression and the protagonist, Tita, who rose up against her mother and tradition for oppressing her as well. The women in the novel and the real women who partook in the Revolution shared traits (i.e. courageous and tenacious) that proved vital to the movement towards women’s rights in Mexico. _____________________ Insights from Shakespeare Professor van Nort’s class Room: University Center 303A ~ SELF-KNOWLEDGE IN OTHELLO AND MACBETH Taylor Warren with Andrea Van Nort (Advisor) Department of English and Fine Arts United States Air Force Academy The protagonists Othello and Macbeth each have a confident self-identity in the beginning of their respective dramas. They define themselves early and are described by other high-minded characters in a like manner, which adds depth and balance to their personas. Equally, their successful military backgrounds bring them an additional dimension, a significant component to the ways in which they view themselves. However, over the course of the plays, others’ readings of them as characters shift and not only distort the way the audience sees them, but also alter the way Othello and Macbeth see themselves. This paper will discuss how, in response to these metamorphosing perspectives of the self, Othello and Macbeth no longer believe in their identities. We will consider as well how their subsequent loss of a firm sense of self will provide the catalyst that enables them to be capable of evil. ~ 15 ~ ~ ANALYZING FORMS OF LOVE IN OTHELLO AND KING LEAR Ashley Christ with Andrea Van Nort (Advisor) Department of English and Fine Arts United States Air Force Academy Shakespeare presents paternal love and romantic love in the two tragedies Othello and King Lear in order to highlight corresponding forms of love illustrated in the Bible. For both plays, love becomes an analogy for several forms of love found in the Bible. Biblical exegesis in this case is also critically interesting because Shakespeare’s world view, rooted in Biblical knowledge, shapes many of the underlying assumptions throughout his works. For this paper we will discuss the paternal relationships that both Brabantio and Lear share with their daughters, Desdemona and Cordelia, and God’s relationship with his son. Then we will illustrate how the romantic relationships between Desdemona and Othello as well as Cordelia and France differ from other Shakespearian marriages, and the way in which their relationships are analogous to Christ’s relationship with the church. ~ THE TWO BODIES OF THE KING EXPLORED Jessica Adams with Andrea Van Nort (Advisor) Department of English and Fine Arts United States Air Force Academy Act III scene ii of Shakespeare’s Richard II, is a moment of twofold importance for the king. He has recently returned from Ireland with confidence in his ability to handle any conflicts which may have arisen in his absence. However, his life-long kingship has bred within his being a false sense of confidence. When he learns of Bolingbroke’s easilystirred mutiny, the willingness of his country to dispose of him, and the loss of the majority of his trusted friends, Richard undergoes a series of realizations which lead to humble confessions. This paper will consider how, in this speech, Shakespeare crafts a distinct idiolect for Richard, resulting in a speech expressing his changing mindset, his eloquent reasoning to lead him towards anagnoresis, metaphor to cause him despair over Death’s control of life, and shifting tone to detail Richard’s altering perspective. ~ 16 ~ _____________________ Revelation Room: University Center 303B ~ THE REVELATION SERIES Patrick Bohnen with Claire Rau (Advisor) Department of Visual and Performing Arts University of Colorado Colorado Springs This talk will cover a recent series of steel sculptures entitled “The Revelation Series”. These sculptures are an exploration of minimalist form, color, and interaction. Presented in this talk will be the process of creating the works, the subtle spirituality within the works, and the influences of the works from Whiteread to Kapoor. ~ FROM AUTOMATA TO ANDROIDS: KARAKURI NINGYO AND THE ART OF JAPANESE ROBOTICS Arianna Gentile Polese Colorado College At first glance, karakuri ningyo may look like simple Japanese dolls, but their charming appearance masks their complexity. Dressed in colorful traditional robes, these diminutive wooden figures are actually small automatons. Powered by a system of handmade gears and springs, these Edo periods clockwork machines are able to perform feats such as carrying tea or painting calligraphy. Karakuri ningyo were expertly built without a single nail, with a precision that is impossible to achieve today. Karakurilike figures have existed in Japan since roughly the ninth century, due to the influence of ancient Chinese technology. But the craft really became more technologically complex during the Edo period, when Portuguese Jesuits introduced clockwork devices to the Japanese. The resulting tradition of karakuri ningyo has influenced the evolution of Japanese robotics up to the modern day, and the interplay between Shinto beliefs and mechanical creatures lies at the root of the Japanese attitude towards robots. ~ 17 ~ ~ THE LIFE OF ARTWORK OF DIEGO VELÁZQUEZ Emily Olson with Dr. Maria Steen (Advisor) Department of Languages and Cultures University of Colorado Colorado Springs Renowned artist Diego Velázquez is one of Spain’s most famous painters. His artistic talent shone through at an early age, giving him the background and experience he needed to become one of the most important artists of the Baroque era. Velázquez introduced many new techniques in his paintings that helped to define his unique, naturalistic style. In addition, he used these techniques to create many masterpieces that are still admired today. This presentation gives a brief biography of Velázquez, explaining his training, education and experience in the court of King Phillip IV. It also examines his naturalistic style and use of revolutionary painting techniques that greatly influenced the world of art. Examples of these techniques are shown in a discussion of Velázquez’s most famous paintings. ~ PICK THAT SHIT UP John Slye Department of Visual and Performing Arts University of Colorado Colorado Springs For my talk, I will be discussing my two major series of work, along with several individual pieces that could turn into series themselves. My first major series of work is titled Quotes from a Memorable Night, and it involves the telling of a short story that helps provide a context for the work. The second series is an examination of the port-apotty graffiti documented at the Construction site that I worked at for several months. I particularly look forward to the question and answer section because I feel that hearing other people’s opinions will help grow my perspective, and my art. ~ 18 ~ Poster Session I (11:00 am-12:30 pm) Room: Berger Hall ~1~ HYDRAULIC FRACTURING: DEVELOPING AN EFFIIENT TECHNIQUE FOR ANALYSIS OF POSSIBLE CHEMICALS FOUND IN HYDRAULIC FRACTURING FLUIDES Eun Hee Kim with Professor Janel Owens (Advisor) Department of Chemistry University of Colorado Colorado Springs Hydraulic fracturing, or fracking, is a method of extracting natural gas from rock by applying pressurized fluid. The hydraulic fracturing is known to contain some chemicals that can have an impact on the environment. The objective of this experiment was to create an efficient technique utilizing green chemistry for analyzing possible chemicals found in the hydraulic fracturing fluids. Headspace liquid phase microextraction (HS LPME) was used to extract benzene, toluene, ethylbenzene, xylenes, naphthalene, bronopol, 2-butoxyethanol and gasoline in water by using 1-octanol as solvent. The experimental conditions were dependent on the concentration of analyte, volume of water used, amount of sodium chloride added to sample, the temperature of the sample, distance of solvent from the sample in headspace, and the amount of 1-octanol utilized. The samples were analyzed by gas chromatography-mass spectrometry (GC/MS) and the data analyzed using AMDIS (automated mass spectral deconvolution and identification software). ~2~ A PSYCHOLOGICAL PROFILE OF ROBERT MUGABE, PRESIDENT OF ZIMBABWE Tessa Holme with Professor Fred Coolidge (Advisor) Department of Psychology University of Colorado Colorado Springs Mayer (1998) suggested a new form of disorder known as ‘Dangerous Leader Disorder’ as a way to differentiate dictators from leaders without pathology. The present study attempted to determine whether Robert Mugabe, President of Zimbabwe, met Mayer’s ~ 19 ~ criteria. Eight educated adult Zimbabwean citizens anonymously completed an online survey of 250-items, the Coolidge Axis II Inventory (CATI), about Mugabe. A consensus profile was compiled and compared to Coolidge et al.’s previous studies of Adolf Hitler, Saddam Hussein, and Kim Jong Il. The results showed that Mugabe’s overall personality disorder profile was the most similar to Saddam Hussein (Pearson ProductMoment Correlation r = .88), which reflected strong sadistic traits, followed by narcissistic, antisocial, and paranoid traits. ~3~ ANALYSIS OF PHARMACEUTICALS BY DISPERSED LIQUID-LIQUID MICROEXTRACTION Cynthia Ortega with Professor Janel Owens (Advisor) Department of Chemistry University of Colorado Colorado Springs The presence of pharmaceuticals in the environment is of growing concern. Thus, a method for determining the amounts of bioaccumulation of carbamazepine, 17α-ethynyl estradiol, and diclofenace was developed. Dispersed liquid-liquid microextraction (DLLME), which is environmentally friendly and experimentally effective, was utilized to extraction the pharmaceutical components prior to instrumental analysis. The following parameters were tested to determine extraction efficiency: salt levels, varying pH, varying dispersive solvents, varying amounts of chosen dispersive solvent, varying extraction solvents, varying amounts of chosen extraction solvent, and varying the sonication time. Analysis by high performance liquid chromatography (HPLC) with UVVis detection was then performed. The percent recoveries were improved, with the following parameters optimized: no salt addition, pH adjustment to 2, ethanol (50 μL) used as dispersive solvent, chloroform (200 μL) as the extraction solvent, and 6 min sonication time. ~4~ METHOD DEVELOPMENT FOR DISPERSIVE LIQUID-LIQUID MICROEXTRACTION OF FENTANYL FROM AQUEOUS SOLUTIONS Michael Gardner with Professor Janel Owens (Advisor) Department of Chemistry University of Colorado Colorado Springs A method for the detection of fentanyl from an aqueous solution using dispersive liquidliquid microextraction (DLLME) has been developed. Fentanyl is a synthetic opiate that is about 80× more powerful than morphine. Fentanyl has proven to be useful for the ~ 20 ~ treatment of extreme chronic pain, such as the pain experienced by terminal cancer patients. Unfortunately, like other opiates, fentanyl also possesses a high potential for abuse. This creates a need for fast, precise, and accurate measurements of fentanyl in a forensic setting. Traditional liquid-liquid extraction and solid-phase extraction can take hours to complete. The method developed here, DLLME, takes approximately 15 minutes to complete. In addition to the time saved through this method, it is also a green method. Traditional extraction methods use large amounts of organic solvents but this method is done on a microliter scale, drastically reducing the amount of organic solvent used. ~5~ PEBBLE NANOSENSORS FOR THE MEASUREMENT OF INTRACELLULAR MAGNESIUM ION CONCENTRATION IN VIVO Kyle Buchwalder, Merritt Logan with Professor Murphy Brasuel (Advisor) Colorado College Magnesium ion is essential for cellular function. This divalent cation plays an important role in enzymatic reactions, maintenance of ribosomal and cytoskeletal structural integrity, energy metabolism and the synthesis of proteins and nucleic acids. Mg2+ PEBBLE nanosensors provide a noninvasive means of monitoring intracellular magnesium ion concentration in vivo. These nanosensors are composed of modified Coumarin 343 magnesium ion sensitive fluorophores and Quantum dot reference fluorophores entrapped within a polyacrylamide matrix. Research in professor Murphy Brasuel’s lab has focused on the development of new synthesis procedures aimed at optimizing real time, dynamic PEBBLE nanosensor measurements of magnesium ion concentration in the model organism D. discoideum. Problems involving Quantum dot reference fluorophore fluorescence and PEBBLE nanosensor delivery to D. discoideum led to multiple modifications of the synthesis procedure. Specifically, Quantum dot reference fluorophores were replaced with Alexa fluor 555 fluorophores and a functionalization procedure was developed which attaches yeast extract molecules to the nanosensors. The addition of new reference fluorophores has allowed us to produce PEBBLE nanosensors capable of providing accurate measurements of Mg2+ in free solution. Furthermore, the addition of a functionalization procedure has produced nanosensors that can be effectively delivered to D. discoideum via phagocytosis. ~ 21 ~ ~6~ INVESTIGATIONS OF DYSBIRINOGENS: MEASUREMENT OF SIALIC ACID ON FIBRINOGEN MOLECULES Nicole Fazio Colorado College Fibrinogen is activated to fibrin to form the blood clot in the blood coagulation cascade. Decreased blood clotting function, in cases of liver disease such as cirrhosis, is associated with fibrinogens with altered structures, called dysfibrinogens. Dysfibrinogens often have increased amounts of sialic acid per fibrinogen molecules. This hypersialylation causes increased clotting time and decreased clot lysis time. We used the thiobarbituric acid assay (Warren 1959) to determine the number of sialic acids per fibrinogen molecule. We hypothesized that cirrhosis patient fibrinogen samples will have higher amounts of sialic acid than normal human fibrinogen. Our results showed variation in the amount of hypersialylation of dysfibrinogens. Determination of the extent of hypersialylation if dysfibrinogens may lead to increased understanding of the altered structure of dysfibrinogens and how this affects interactions with other blood coagulation factors as well as with clot formation. ~7~ THE IMPACT OF DEMOCRACY ON INDUSTRIAL GROWTH IN POST-COMMUNIST RUSSIA Emily Freeh, Jessica Nichols with Professor Cerian Gibbes (Advisor) Department of Geography and Environmental Studies University of Colorado Colorado Springs With the end of the Cold War and the fall of the Iron Curtain in 1989, communist Russia was ripe for economic, political, and social revolutions. The dissolution of the USSR allowed for Russia to be able to internalize these problems and find the means to fix them. Russia declared itself a democracy in December, 1993 which helped the country return to power after the stigma of the Cold War. With the political change in government, from communism to a democracy, how has the industrial landscape been impacted? To address this question, Landsat imagery will be used to analyze the industrial changes in Moscow from the end of the Cold War to present day. The primary focus of this examination will be the temporal and spatial relationships in the industrial arena, and its effects on the changes of the landscape. It will also provide clues to Russia’s and Moscow’s true economic and political health through continued progress and develop. Ultimately, this report will help determine whether the transition to a democratic government has an effect on the economic landscape of this already welldeveloped country. ~ 22 ~ ~8~ APPLICATION OF METEOROLOGICAL INSTRUMENTS ON REMOTELY PILOTED AIRCRAFT Gregory W. Hanson with Lt. Col. Christian S. Wohlwend (Advisor) United States Air Force Academy The ultimate goal of this research project is to provide real-time weather data collected from aircraft to ground based or airborne users. This data will be outputted in a user friendly manner that allows for ease of use. This project is a continuation of an earlier project that demonstrated small weather sensors could easily be built and placed on Remotely Piloted Aircraft (RPA). The team will now be focusing on using the wealth of data produced daily from preexisting manned aircraft and RPAs to explore the applicability and utility of using this data in real-time to accurately plan military operations. It will be crucial to output the data in an easy to read format that also allows for a graphical representation of the atmosphere to aid in operations such as precision air drop. The output application will also be compatible with various Air Force aircraft sensor configurations. Future research will take the same data and input it into high resolution numerical models that will aid in forecasting in complex terrain such as the Colorado Front Range. ~9~ MALWARE IDENTIFICATION THROUGH BASIC BLOCK ANALYSIS Frank Adkins, Luke Jones United States Air Force Academy Each day, antivirus companies are flooded with more malware than they have the ability to handle. Modern antivirus programs generate a new signature for every one of these samples and signature databases have grown at an exorbitant rate. However, most of this “new” malware is just a slight change from other samples that have been observed before. Both the old and new samples have essentially the same functionality. Thus, it makes sense to stop detecting malware by signatures, and start weeding them out by functionality. This is known as heuristics-based detection. The purpose of this research is to introduce an algorithm that improves on existing heuristics-based malware detection techniques. The improvements described in this paper act by identifying specific malicious functionality in binary executables. This analysis is done through a tool called BBOB. This tool can be used to classify binary executables as malware based on the functionality they contain. The experimental results show that BBOB is able to detect shared functionality between executables and has the potential to serve as an innovative model in malware detection. ~ 23 ~ ~ 10 ~ MODELING AND REEVALUATING PERFORMANCE METRICS AND THE DETOX SYSTEM IN DENVER EMERGENCY MEDICAL RESPONSE SYSTEM DJ Cherf, Joe Han, Dan Pickett, Jeff Williams with Capt. Brian Lemay and Capt. Matt Wroten (Advisors) United States Air Force Academy The City of Denver uses National Fire Protection Agency measures to assess the performance of its emergency medical response system (EMRS). The current standard only uses response time—the time from when a call is received to when help arrives on the scene. Many experts question the validity of the standard since only a small portion of the medical calls require the immediate attention on which the standard is based on. Also, the current standard encourages medical personnel to drive at potentially unsafe speeds. This study analyzes the current system and recommends a comprehensive performance metric to better measure the performance of the emergency medical personnel. Additionally, this study addressed the resource allocation problem where the emergency medical response personnel spend a lot of time transporting intoxicated patients. Denver does have detox vans that can transport those patients to Denver Care, the detox center, but not enough to arrive on scene in 15 minutes, which is the legal threshold for the paramedics to transport them to the ER room. This project’s goal is to model the system and come up with the optimal number of detox vans during various times of the day. ~ 11 ~ DESIGN CONSIDERATIONS IN A HYBRID MOBILE ENERGY STORAGE AND GENERATION SYSTEM Ryan DeCarlis, Wes Cobb, Zane Dydasco United States Air Force Academy Mobile energy systems employing renewable sources are not a new idea. Many companies have developed and sold them for several years. Most use solar panels while a few utilize small wind turbines as primary generation sources. Our senior capstone design team has investigated and developed a mobile hybrid energy storage and generation system capable of supplying 18kwh of electricity and that integrates multiple generation sources including wind, solar, and human powered mechanical. Our system is capable of storing and delivering approximately18kwh at various voltages including 120 and 240VAC, 3-phase AC, and can even charge a cell phone via a USB port. This flexibility makes our system unique highly desirable in a field deployed location. This focus of this paper, however, is on the systems engineering aspect of the design. We describe our design process including false starts, problems encountered, and how our schedule, budget, and resource management practices evolved over the ~ 24 ~ two semesters of the course. Our team consisted of 8 cadets with 5 different engineering related majors: Electrical(2), Computer(1), Systems(2), Management(1), and Human Factors(2). Each cadet was assigned specific areas of the design beginning with current practices research in their area of expertise and ultimately each contributed to the project in a meaningful way. ~ 12 ~ QUENCHING OF FLUORESCENT PROTEINS BY DRUGS OF ABUSE Alexa M. Gingras with Barry W. Hicks (Advisor) Department of Chemistry United States Air Force Academy Previous research has confirmed ultra-violet excitation of fluorescent proteins leads to visible emission by Fluorescence Energy Transfer (FRET). It has also been proven that collisional quenching of the surface tryptophan residues and dynamic quenching reagents can interrupt this FRET pathway and inhibit fluorescence within the protein. This has opened the door to use of fluorescent proteins as biosensors for collisional quenching agents. In this study, the quenching effectiveness of drugs of abuse on fluorescent protein eCGP123 was determined through triplicate analysis. Drug titrations were completed from concentrations of 7.9 to 500 μM to determine the lowest concentration need to achieve complete fluorescence quenching. Our results indicate that 500 μM of morphine and methadone can quench fluorescent protein eCGP123. Further testing of THC, methamphetamine, cocaine, nicotine, and ecstasy will determine if they can achieve the same collisional quenching. ~ 13 ~ PURSUIT OF AN IMPROVED ASSAY FOR HIB-PROTEASE INHIBITORS via FRET FROM A YFP-HIV-1-PR FUSION PROTEAIN Steven Freund with Barry W. Hicks (Advisor) Department of Chemistry United States Air Force Academy HIV-1 Protease has been targeted by antiviral inhibitor drugs in order to slow HIV’s progression to AIDS. Because the PR can be mutated leading to viral resistance, there is a need for new protease inhibitors. Current screens for new PR inhibiting drug candidates are expensive and time-consuming. The purpose of this research is to try and create a less expensive and more efficient method. Recombinant HIV protease will be produced by Chinese Hamster Ovarian (CHO) cells. These are being transiently ~ 25 ~ transfected with pDNA encoding for a YFP-HIV-1 PR fusion protein provided by Dr. Badley at the Mayo Clinic. The cells will be grown in the presence of one of six PIs (Atazanavir sulfate, Indinavir sulfate, Amprenavir, Nelfinavir, Tipranavir, & Darunavir) provided by NIH and Bristol-Meyers Squibb. Following isolation of the PR, a binding assay with each of the PIs will be done using Fluorescence Resonance Energy Transfer (FRET). The extent of quenching by the inhibitors may indicate their binding affinity and the overall efficacy of the drugs. ~ 14 ~ THE EFFECT OF DIMISHED PRR15 EXPRESSION ON SELECTED GENES Ciani M. Ellison with Ryan W. Maresh (Advisor) Department of Biology United States Air Force Academy The objective of this study was to evaluate the expression of cingulin following the knock-down of PRR15. PRR15 encodes proline-rich 15, a nuclear protein that is important in early embryonic development of most mammals. The expression of PRR15 is dependent upon the availability of nutrients to the placenta during fetal development, and has an epistatic effect on the expression of other genes including cingulin, which is associated with breast cancer and developing cell density. Using realtime PCR, PRR15 knock down cells were compared to control cells with normal functioning PRR15. Upon quantifying the gene expression of cingulin in response to normal and under expression of PRR15, we conclude that there is no correlation between the knock down of PRR15 and the expression of cingulin. Elucidating the relationship between gene expression and placental insufficiency will help facilitate understanding of how the fetal environment influences the onset of certain diseases in adulthood. ~ 15 ~ METALLIZED PERFLUOROPOLYETHER BLENDED URETHANE COMPOSITES Nicholas A. Clayton with Sharon C. Kettwich and Scott T. Iacono (Advisors) Department of Chemistry United States Air Force Academy Poly(urethane)s (PUs) are a versatile class of tailorable, step-growth polymers used for a plethora of material applications. PUs backbones can be modified to possess soft and/or hard block segments to adjust processing and bulk mechanical properties. This work utilizes PUs as a host matrix for blending perfluoropolyether (PFPE) coated aluminum core-shell particles. PFPEs have been shown to undergo metal-mediated ~ 26 ~ degradation producing thermodynamically favorable oxidized aluminum species, in this case, aluminum fluoride. Processing and characterization of the PU blended composites using TGA, DSC, and ATR-IR will be presented. ~ 16 ~ NETWORK SECURITY AND ITS RELATION TO SOCIO-ECONOMIC STANDING Taylor Watson, Matthew Howard United States Air Force Academy With the rise of wireless communications, a need for wireless security has been created. Along with this new attempt to secure wireless networks, an activity known as war driving has been gaining popularity. The purpose of war driving is generally to gain information and statistics about wireless local area networks (WLANs), but recent years have also seen a rise in nefarious activities using war driving. War driving can be used to gain information about the security of a WLAN, which can then be compared to the socio-economic status of the household or business. The purpose of this study is to provide a detailed analysis of the correlation between socio-economic status and wireless security which could lead to new insights about network security. This information could be potentially useful in future efforts to educate the general populace in basic network security principles and prevent a number of cybercrimes from occurring. ~ 17 ~ ANALYSIS OF RETAINED AUSTENITE IN 440C STEEL BY X-RAY DIFFRACTION Ralph W. Bush, Jarred W. Teakell Department of Chemistry United States Air Force Academy It has been shown that high alloy martensitic steels such as 440C stainless steel retain substantial amounts of austenite within the matrix following quenching which reduce the strength and ductility of the steel after tempering. Several heat treatment techniques can be used to reduce the amount of retained austenite present, improving mechanical properties such as hardness and ultimate strength. The standard method used to measure retained austenite content is X-ray diffraction. However, stainless steel alloys such as 440C contain a significant volume percentage of chromium carbide phases whose X-ray peaks can overlap with the retained austenite peaks and make interpretation of the X-ray diffraction data very difficult. This investigation was conducted to validate the use of X-ray diffraction techniques to determine the amount of retained ~ 27 ~ austenite in high alloy steels with substantial carbide content, and to determine the most effective techniques to reduce the amount of retained austenite in 440C steel. In addition, analysis was conducted via scanning electron microscopy and energydispersive X-ray spectroscopy to determine the effects of austenizing temperature and heat treatment techniques on both the chromium and carbide content of the matrix. ~ 18 ~ EUTECTIC IONIC LIQUID MIXTURES USED FROM FORENSIC SAMPLING OF EXPLOSIVES Yasmin I. Sarmiento with Hannah A. Miller, Joseph A. Levisky, Scott T. Iacono (Advisors) Department of Chemistry United States Air Force Academy Homogenous eutectic ionic liquid mixtures consisting of a hydrophobic and hydrophilic component were created in an effort to maximize the range of analytes to be collected, stored, and analyzed quantitatively and qualitatively. The approach of this study included tests for homogenous eutectic ionic liquid mixtures and solubility tests with explosives, which were then analyzed via direct analysis in real time—mass spectrometry (DART-MS). Analyte peaks were well-defined and had no observed matrix interference from the collection swab, suggesting that the ionic liquid mixtures were able to not only retain the analyte, but also preserve the sample located on the swab. These preliminary findings show potential for new forensic sampling processes that optimize the collection, preservation, and recovery of field evidence. ~ 19 ~ PREPARATION OF PERFLUOROPOLYETHER PASSIVATED ALUMINUM BLENDED EPOXY RESINS James W. Neat II with Hannah A. Miller, Scott T. Iacono, Sharon C. Kettwich (Advisors) Department of Chemistry United States Air Force Academy Epoxy resins possess robust mechanical properties and are desirable for preparing blended composites for high performance applications. Oligomeric perfluoropolyethers (PFPE) have been employed as bulk surface property modifiers when blended in hydrocarbon polymer systems. In this work, fluorinated epoxy resin formulations were prepared by mechanically blending PFPE coated nanometer-sized aluminum particles ~ 28 ~ with bisphenol-epoxy prepolymers as the host matrix. The methodology development, cure kinetics, thermal activation, and surface properties of these composites will be presented. ~ 20 ~ ANTIBACTERIAL PROPERTIES OF HIPPOPOTAMUS AMPHIBIUS GLANDULAR SKIN SECRETION Katrina Litchford, Amanda Marshall, Keeley McCann with Ilea Eskildsen (Advisor) Department of Biology United States Air Force Academy Secretion of "hippo sweat" from the skin is believed to be effective as a sunblock, insect repellent, and antibiotic for the hippo Hippopotamus amphibius. The purpose of the study was to test the antibacterial properties of this secretion by using the Kirby-Bauer method for antimicrobial susceptibility. The samples of the "hippo sweat" that were used during testing were collected by the zoo keepers at the Cheyenne Mountain Zoo. Different microbes were used to test the range of effectiveness of the Hippopotamus amphibius secretion. After testing on Mueller-Hinton agar plates, the zones of clearance were examined to evaluate antibacterial activity. Integumentary secretions of the Hippopotamus amphibius were found to have antibacterial effects against the Gram positive bacteria Staphylococcus aureus and Streptococcus pyogenes (p <0.001), but not against the Gram negative bacteria Kleibsiella pneumoniae and Serratia marcescens. Although the exact mechanism of action is unknown, these data suggest that the antibacterial properties may be due to interactions with bacterial components specific to Gram positive bacteria, potentially the peptidoglycan cell wall, or the antibacterial properties are rendered ineffective by properties of Gram negative bacteria, such as the lipopolysaccharide layer. Identification and characterization of the antibacterial components of "hippo sweat" may provide insights for the development of new antibiotics. ~ 29 ~ ~ 21 ~ SYNTHESIS OF A TNT BIOSENSOR: EVALUTION OF THE APPLICABILITY OF A SHORT-CHAIN PEPTIDE THROUGH FRET QUENCHING Taeyoung Lee with Barry W. Hicks (Advisor) Department of Chemistry United States Air Force Academy Detection of illegally transported explosives has been a critical aspect of national security since the September 11th attacks on American soil. Trinitrotoluene, TNT, is the most common explosive used by arms producers. Despite the need to have real time TNT detection, existing detecting technology is slow, expensive and cumbersome. Recent research demonstrated that nitroaromatic explosives and degradation products can be detected by quenching of Förster or fluorescent resonance energy transfer (FRET) from endogenous tryptophan to the chromophore of fluorescent proteins (FPs). However, selectivity of the FP sensors is not very good as any nitrotoluene (NT) leads to quenching. In this work, TNT selective peptides will be modified with an allyl moiety in order to synthesize an acrylamide co-polymer coating to be used as a highly selective and sensitive TNT biosensor. Quenching of the protein will be tested step-wise throughout the modification and synthesis processes through fluorescence spectroscopy and quantified by Stern-Volmer plots. Our results will indicate fluorescence quenching will increase as higher concentration of TNT solution or vapor is exposed to the peptide; in fact, the mean Stern-Volmer constant (KSV) for TNT was 23100 M-1, indicating much better quenching than free tryptophan with 6000 M -1. ~ 22 ~ COMPARATIVE MORPHOLOGY OF CEREBELLAR CORTEX NEURONS IN CLOUDED LEOPARD, SIBERIAN TIGER, HUMPBACK WHALE, AND CHIMPANZEE Nicholas Johnson with Chet Sherwood, Paul Manger, Bob Jacobs (Advisors) Laboratory of Quantitative Neuromorphology, PsychologY Colorado College Despite extensive investigations of the gross anatomy of the cerebellum, its connections, and qualitative neuromorphology, little quantitative neuromorphological information exists, particularly on species outside rodentia. To that end, the present study quantitatively investigates, for the first time, the cerebellar neuronal morphology of the clouded leopard (Neofelis nebulosa), Siberian tiger (Panthera tigris altaica) humpback whale (Megaptera novaengliae), and chimpanzee (Pan troglodytes). Neurolucida tracing software (MBF Bioscience) was used to three-dimensionally trace ~ 30 ~ cerebellar neurons (N=198) stained by a modified rapid Golgi technique: stellate (n=50), basket (n=40), Golgi (n=31), Lugaro (n=35), and granule neurons (n=42). Qualitatively, traced neurons appeared similar to those described in other mammals, although they varied in size across species (Palay & Chan-Palay, 1964). Across species, basket and stellate neurons generally increased in size (e.g. total dendritic length and number of dendritic segments) in the following order: clouded leopard < Siberian tiger < humpback whale < chimpanzee. In contrast, Golgi, Lugaro, and granule cells increased in size across species in the following order: chimpanzee < clouded leopard < humpback whale < Siberian tiger. Several molecular layer neuronal measures were also positively correlated with cerebellar volume (e.g. total dendritic length, average length of dendritic segment), whereas granular layer neuronal measures were not significantly correlated with cerebellar volume. Although there were no consistent species-specific quantitative patterns in the present results, the basic morphology of neurons in examined species suggests that cerebellar cortical neurons are evolutionarily conserved within mammals. ~ 23 ~ SYNTHESIS OF CF₂H ANALOGUES OF 4-AMINOQUINOLINES Kameron Montana Colorado College Malaria is a deadly tropical disease currently infecting an estimated 216 million people worldwide with an annual mortality rate between 700,000 – 1,000,000. Many of the current antimalarial drugs have adverse side effects. Therefore, the search for new and effective antimalarial compounds is vital. Amodiaquine, an antimalarial belonging to the class of 4-amino quinolines, exhibits harmful side effects that are associated with metabolic oxidation of the para aminophenol group. In an attempt to eliminate this oxidation, a CF2H analogue of Amodiaquine has been proposed. This analogue is expected to eliminate the metabolic oxidation pathway while retaining anti-malarial efficacy. The first four steps of an eight step synthesis have been performed successfully. ~ 31 ~ ~ 24 ~ A NOVEL MUTATION IN THE daf-19 GENE AFFECTS CILIATED NEURON DEVELOPMENT IN C. ELEGANS Kristen Wells with Dr. Darrell Killian (Advisor) Colorado College The Caenorhabditis elegans male and hermaphrodite nervous systems display sexually dimorphic development characterized, in part, by the presence of 8 hermaphroditespecific neurons and 89 male-specific neurons. We are interested in identifying the genes and molecular mechanisms that govern sex-specific neural development in C. elegans. Through a mutagenesis screen using a pkd-2::GFP reporter to label malespecific neurons, we recovered several mutants that display defects in sex-specific neural development. Males carrying the sm129 mutation lack pkd-2::GFP expression in the male-specific CEM neurons that are involved in mate finding. Genetic epistasis experiments suggest that CEM neurons are improperly specified or differentiated. We cloned the sm129 mutation and determined that it is an allele daf-19 based on three pieces of evidence: (1) RNAi of daf-19 phenocopies sm129, (2) sm129 fails to complement a daf-19 null mutation, and (3) we found a mutation in daf-19 that likely affects splicing. We are also testing to see if sm129 mutants can be rescued by adding a wild type copy of daf-19. daf-19 encodes an RFX transcription factor that activates genes required for sensory cilia function in ciliated neurons such as the CEMs. daf-19 null mutants lack all sensory cilia, have sensory defects, and display a constitutive dauer phenotype (worms enter an alternative part of the lifecycle associated with starvation survival). We are currently investigating how this mutation affects ciliated neurons such as CEMs but does not affect dauer formation. ~ 25 ~ THE GIFT OF GIVING: PHILANTHROPY AS A PATH TO SELF-ACTUALIZATION Alexander Glassman with Tomi-Ann Roberts (Advisor) Colorado College Giving is generally understood as a personal sacrifice that benefactors endure for the good of society. However, a growing amount of research suggests that the relationship between givers and recipients is much more mutually beneficial than is generally believed. This paper examines literature that illustrates how incredibly rewarding giving can be, how this joy is distinct from other experiences such as compassion, and how giving is deeply rooted in the human psyche. The evolutionary basis for this ~ 32 ~ phenomenon may be primarily based on Triver's (1971) conception of reciprocal altruism, which suggest that prosocial giving promotes reciprocal relationships which ultimately make both parties more evolutionarily fit. This paper then proceeds to use affective forecasting literature to draw conclusions about why we generally fail to predict that giving will be emotionally rewarding. Finally, and perhaps most importantly, the paper references literature which suggests that giving offers much more than a simple hedonic boost; it provides the opportunity for people to fulfill their desire to reach their full potential, to self-actualize. ~ 26 ~ SERVICE LEARNING: FRIENDS OF FOUNTAIN FAIRVIEW CEMETERY PROJECT Kim Sweetwood, Maria Tobin with Barb Headle (Advisor) Department of History University of Colorado Colorado Springs In summer of 2012, the UCCS department of history held a class, “Cemeteries, Legacies, and American History,” which focused on getting Historians out of libraries and into the field using cemetery markers as primary source documents. With permission from the City of Fountain, Colorado the class was able to use the Fairview Cemetery in Fountain as a lab in which to map the cemetery with a collaborative guest lecture from the Geography Department. A month after the class ended, vandals destroyed several of the oldest and most irreplaceable headstones. In response, a student initiated committee, including UCCS Faculty and students from both the history and geography departments, and civic organizations and community leaders from Fountain, teamed up to raise awareness against cemetery vandalism and funds for headstone restoration and a security system. The committee chose to host the First Annual Friends of Fountain Fairview Cemetery “Crawl” and Silent Auction which successfully raised more than double the original goal. Thus began an odyssey in service learning that has, at the request of the City of Fountain, become a highly anticipated annual event. This service learning event was the perfect blend of faculty, student, and community cooperation for a most worthy cause. ~ 33 ~ ~ 27 ~ A COMPARISON OF RUN-AWAY AND NON-RUNAWAY ADOLESCENTS’ SELF-ESTEEN, TIME PERSPECTIVE, AND GENDER Anne Marie Saxon, Isaac C. Wiggers, Frank C. Worrell with Professor Zena R. Mello (Advisor) Department of Psychology University of Colorado Colorado Springs Little is known about adolescents who run away from home (i.e. runaways) and the risk factors associated with them. Runaway adolescents are an under-researched and vulnerable population. Every year, between 1.6 and 2.8 million adolescents leave their home (National Runaway Switchboard, 2010). They have been found to have higher anxiety, depression, suicide rates, as well as lower health, nutrition, and self-esteem (National Conference of State Legislation, 2010). Studies also suggest that when compared to their non-runaway counterparts, runaway adolescents are at a significantly greater risk for medical problems and health compromising behaviors that include HIV and other sexually transmitted and infectious diseases; substance abuse; psychotic behavior, depression, suicide attempts, prostitution, and trauma (Greene et al., 2003). Adolescents who run away from home face a multitude of challenges to healthy development. It is important to identify psychological factors that may impact these adolescents so that effective interventions can be implemented. Recently, Mello (2013) described how time perspective—thoughts about the past, the present, and the future— was associated with numerous indicators of health, such as self-esteem and substance use. Thus, in this study we sought to bridge the literatures on runaways with time perspective by addressing the following two research questions: (a) how do runaway and non-runaway adolescents differ in time perspective? (b) how does variation in time perspective predict engagement in risky-behaviors for runaways and non-runaways? ~ 28 ~ VARIATION IN BRAIN RESPONSES TO EMOTIONAL STIMULI AS A FUNCTION OF REPRODUCTIVE HORMONES: COMPARISON OF NATURALLY CYCLING WOMEN TO THOSE TAKING ORAL CONTRACEPTIVES Polina Reynolds with Mike Kisley (Advisor) Department of Psychology University of Colorado Colorado Springs Perceptual differences, particularly those related to courtship and mating, have been discovered across the menstrual cycle. Oral contraceptives (OCs) are used by a significant portion of childbearing age women to alter their cycles. This study assessed ~ 34 ~ event related potentials (ERPs) and evaluative categorization responses to, and subjective ratings of images with and without reproductive significance in women who were naturally cycling and those on monophasic formulations of hormonal birth control. Both groups were tested during days 13-20 (ovulation and luteal) of their cycle and previous 3 months of menstrual cycle data was used to estimate day of ovulation for study cycle and control for progesterone level. The IAPS database was used for all images; neutral people served as context images and three categories of images with positive valence were chosen as targets (men, babies, and romance). Images were presented to participants to categorize as not positive, somewhat positive, or very positive with targets presented pseudo-randomly among neutral people images. Quantitative SAM ratings of valence and arousal were obtained for each of the target images at the conclusion of the ERP measurements. P300 amplitudes were compared across groups and no effect of OC use on processing of reproductive stimuli was found. There was a trend toward reduced ERP amplitude in response to images of men with OC use, and this should be further investigated with a larger sample size and more rigorous control of menstrual cycle day. ~ 29 ~ HEXOKINASE II INHIBITOR DETERMINATION AND ENZYME KINETICS USING ULTRAVIOLET-VISIBLE SPECTROSCOPY Liz Lata, Mary Bennes with Professor Sonja Braun-Sand (Advisor) Department of Chemistry University of Colorado Colorado Springs The rate of cancer cell growth exceeds the rate at which blood vessels can supply cells with oxygen, rendering an oxygen-deficient environment. Therefore cell growth and reproduction relies heavily upon glycolysis as the prime resource for adenosine triphosphate (ATP). Hexokinase catalyzes phosphorylation of glucose, the first irreversible step in the glycolytic pathway. The discovery of a competitive or noncompetitive inhibitor to hexokinase could theoretically restrict supply of energy to cancer cells, resulting in apoptosis or necrosis. More specifically, the over-expression of human hexokinase II in several cancers emphasizes the need to find an effective inhibitor of this particular isoenzyme. Understanding differences between the yeast isoenzymes is an initiation point for comprehending variances in isoenzyme function. Enzyme kinematic studies were performed using Hexokinase PII (HKPII) from Saccharomyces cerevisiae, and the maximum velocity (Vmax) as well as the Michaelis constant, Km, were determined for the enzyme using a UV-vis spectrophotometer. ~ 35 ~ ~ 30 ~ DIFFERENTIAL SCANNING CALORIMETRY INVESTIGATION OF YEAST HEXOKINASE TYPE II Malia Malihi University of Colorado Colorado Springs A doubled-peak endotherm with maxima in the heat capacity curve, T m, located at 45.5 ±0.1 and 50.0 ±0.1 °C was observed for hexokinase type II (HKII) at pH 8.5 in 5 mM Tris buffer that was similar to the endotherm with maxima at 41 and 48 °C reported by Takahashi et al.[1]. The ratio of the calorimetric enthalpy change, ∆Hc, to van’t Hoff enthalpy change, ∆HvH, was 2.0 ± 0.1 for the lower temperature transition. However, the van’t Hoff enthalpy change was up to 10 times larger than the calorimetric value for the higher temperature transition. These results suggest that the two domains denature independently followed by an endothermic self- association. A slightly asymmetric endotherm with a small shoulder located on the high temperature side of the T m, ∆Hc/∆HvH = 0.9 + 0.2, was observed at pH 7.5 – 8.5 in 50 mM HEPPSO. The Tm ranged from 46.7 °C at the lower pH to 43.1 °C at pH 8.5. However, an endotherm with a pronounced shoulder located on the low temperature side of the transition, T m = 46.7 °C, was reported by Catanzano et al. for HKII in the same buffer, with ∆Hc/∆HvH = 2:1[2]. A different mechanism for denaturation was proposed than the one cited above. ~ 31 ~ GENETIC REGULATION OF CLASS IV DENTRITIC ABORIZATION NEURONS IN DROSOPHILA MELANOGASTER Mary Morton with Professor Euenia Olesnicky-Killian (Advisor) University of Colorado Colorado Springs Class IV dentritic aborization (da) neurons are pain sensing neurons that are a part of the Drosophila melanogaster larval peripheral nervous system. Class IV da neurons generate ~800 branches termed dendrites that are dedicated to sensing pain. Once a larvae perceives pain, the animal rolls in order to escape from the painful stimuli. The Killian lab has found that by inactivating the function of several genes including cg11505, shep, bancal, caper, pitchoune, and meicp26, it results in significantly decreased dendrite branching. Since dendrites mediate pain sensation, this may suggest that pain sensation is compromised in animals lacking functional cg11505, shep, bancal, caper, pitchoune and meip26. A series of behavioral analysis experiments were conducted in which various lines of animals with each gene inactivated separately were poked and the response was recorded as ether a roll or no roll. A roll indicated that a sufficient amount of dendrites were present in order to react to the pain sensation; ~ 36 ~ conversely, no roll indicated that an insufficient amount of dendrites were present and the larvae failed to escape from the stimuli. My results show that each mutant showed a decreased roll percentage when compared to a control thus indicating that each gene plays a role in both the development of dendrites and facilitating escaping behavior. ~ 32 ~ JUMPSTARTING STEM EDUCATION: SPARKING ATTITUDES AND INTENTIONS IN UNDERSERVED STUDENT POPULATIONS Jonathan G. Pugh, Peter D. Marle, Lisa L. Decker with Dave H. Khaliqi (Advisor) Center for STEM Education University of Colorado Colorado Springs The UCCS Center for Science, Technology, Engineering, and Mathematics (STEM) Education offers engaging, hands-on STEM programs to primary and secondary school students. As new science standards assert increased diversity in the STEM pipeline, CSTEME programs try to gain increased representation in STEM from historically underserved students. This study examined the equitability of a weeklong STEM program, Jumpstart STEM: The Crooked Circuit (JSCC). JSCC included 11 workshops of varying STEM fields, and each workshop was integrated into the overall scenario, thereby offering relevance to the subject-matter. Thirty-nine students (19 males, 16 females; 4 unreported) completed the post-event survey which contained items measuring science attitudes and future STEM intentions (significant, positive retrospective pre/post increases). JSCC outcomes were then compared to a previous Jumpstart program which was highly equitable and successful. Results suggest the camp garnered equitable outcomes for all students, thus lending to the possibility for increasing diversity in the STEM pipeline. ~ 33 ~ DO ADVERSE CHILDHOOD EXPERIENCES AFFECT THE WAY PEOPLE RESPOND TO DEATH THOUGHTS? Lisa Starcher University of Colorado Colorado Springs Terror Management Theory claims that humans and other living creatures share certain characteristics, including the strong desire to live. According to the theory what sets humans apart is awareness of their mortality. The tension caused by the desire to live and the knowledge of their eventual death causes terror for humans that must be managed. The theory states that we use what is called an Anxiety Buffer to do just that. ~ 37 ~ The Anxiety Buffer is comprised of one’s cultural world view, self-esteem, and close interpersonal relationships. This anxiety buffer begins to form as soon as one is born in relation to the interactions of the infant and their parents or caregivers. Previous research revealed a correlation between adverse childhood experiences and issues in adulthood that are physical, behavioral, and psychological in nature. The purpose of the present study was to determine if the reason people with adverse childhood experiences are more likely to die from cancer, use illicit drugs, or be diagnosed with PTSD is a direct result of an Anxiety Buffer that did not form properly, because of those childhood experiences. It was hypothesized that adverse childhood experiences would alter the way people responded to a death reminder. ~ 34 ~ CHARACTERIZATION OF A GENETIC MUTANT IN DROSOPHILA MELANOGASTER Hannah Steinert with Professor Jeremy M. Bono, Professor Eugenia Olesnicky-Killian (Advisors) Department of Biology University of Colorado Colorado Springs Although very little is known about the conserved RNA binding protein Cg11505, work in the Killian lab at UCCS has demonstrated that the mRNA is expressed in the central nervous system during development of Drosophila melanogaster and is required for formation of dendritic and axonal structures and may function in stem cell development. RNA interference (RNAi) has been used to disrupt expression of cg11505, but is not effective enough to eliminate all expression. The purpose of our work was to generate a mutant allele of cg11505 using a technique termed imprecise p-element excision. Over 100 potential mutant lines were initially generated, 2 of which were ultimately characterized in depth to determine whether or not a bona fide cg11505 mutant was generated. To determine if these two lines of mutant D. melanogaster, cg11505 del 69A and cg11505 del 69B, had disrupted the cg11505 gene, polymerase chain reaction (PCR) and gel electrophoresis were performed and the mutation was verified through sequencing. We found that cg11505 del 69B experienced no disruption, while the mutation incurred in cg11505 del 69A completely removed the cg11505 gene and as well as two genes cg17746 and cg12078. ~ 38 ~ ~ 35 ~ DISPERSED LIQUID-LIQUID MICROEXTRACTON (DLLME) OF CHLOROGENIC ACID FROM PEACH JUICE Santiago Bukovsky-Reyes with Professor Janel Owens (Advisor) Department of Chemistry and Biochemistry University of Colorado Colorado Springs Although very little is known about the conserved RNA binding protein Cg11505, work in the Killian lab at UCCS has demonstrated that the mRNA is expressed in the central nervous system during development of Drosophila melanogaster and is required for formation of dendritic and axonal structures and may function in stem cell development. RNA interference (RNAi) has been used to disrupt expression of cg11505, but is not effective enough to eliminate all expression. The purpose of our work was to generate a mutant allele of cg11505 using a technique termed imprecise p-element excision. Over 100 potential mutant lines were initially generated, 2 of which were ultimately characterized in depth to determine whether or not a bona fide cg11505 mutant was generated. To determine if these two lines of mutant D. melanogaster, cg11505 del 69A and cg11505 del 69B, had disrupted the cg11505 gene, polymerase chain reaction (PCR) and gel electrophoresis were performed and the mutation was verified through sequencing. We found that cg11505 del 69B experienced no disruption, while the mutation incurred in cg11505 del 69A completely removed the cg11505 gene and as well as two genes cg17746 and cg12078. ~ 36 ~ Melissa Crowner University of Colorado Colorado Springs In internally fertilizing organisms, mating involves a series of coordinated molecular and chemical reactions that occur within the female reproductive tract. Male accessorygland proteins (Acps) are notably important as they are involved in a multitude of physiological responses in females. Novel discovery of full mRNA transcripts within D. arizonae female reproductive tracts after heterospecific matings with D. mojavensis has led to research into the function of these transcripts. It has been verified that these transcripts are also observed within D. arizonae female reproductive tracts after conspecific matings as well. The fates of the mRNA transcripts once inside the reproductive tract of a con- or heterospecifically mated female will be analyzed via qPCR at two different time points (zero and five hours) post copulation of D. arizonae males crossed with D. arizonae females and D. mojavensis females. Differences in quantity of the transcripts in con-verses heterospecific matings may indicate possible mismatches in the heterospecific crosses and provide insights concerning reproductive isolation and speciation. ~ 39 ~ ~ 37 ~ RELATIONSHIPS BETWEEN VEGETATION INDICES AND IMPERVIOUS EXPANSION TO IDENTIFY VEGETATION BEHAVIOR TRENDS IN COLORADO SPRINGS Rebecca Long, Kami McFall, Jennifer Phillips University of Colorado Colorado Springs Increased population tends to result in increased urbanization over previously natural lands. Expansion of impervious surfaces physically results in a general loss of local vegetation with a negative impact on urban environmental conditions. Urban vegetation planning has often been thought to mitigate this problem. This study will evaluate the impact that urban growth in Colorado Springs has on its local vegetation. Using Landsat Thematic Mapper (TM) imagery over twenty years, impervious classification and Normalized Difference Vegetation Index (NDVI) trends will be analyzed statically using ANOVA to compare and evaluate their relationship. WorldView-2 high-resolution imagery over a defined area of Colorado Springs will be used specifically to target variability in vegetation composition. We anticipate this study will show that NDVI ratios and impervious trends will reveal a relationship between urban growth and vegetation behavior. In particular, we anticipate temporal variability in urban sprawl, and vegetation distribution; we anticipate there will also be a dominance of invasive species in older urban development. Invasive species can overrun and impede local native vegetation growth, thereby, working against the natural progression of native vegetation and undermine urban vegetation planning. Awareness of invasive trend factors can improve future urban vegetation planning. ~ 38 ~ THE EFFICACY OF CREATIVE WRITING INSTRUCTION Sean Beedle with Professor Michelle Neely (Adisor) Department of English University of Colorado Colorado Springs This project endeavors to discover the real, tangible benefits of creative writing coursework and instruction to creative writers. By surveying a variety of creative writing classes, creative writing instructors, and published writers I hope to understand the relationship between the study of creative writing and the ability to produce engaging, entertaining, publishable works of modern literature. ~ 40 ~ ~ 39 ~ BRIDGING THE GAP BETWEEN THE ACADEMIC AND ATHLETIC ENVIRONMENTS FOR STUDENT-ATHLETES Heather Sanders with Professor Michelle Neely (Adisor) Department of English University of Colorado Colorado Springs My study explores how female collegiate athletes manage their time between academic, athletic, and personal focuses. To understand what kind of adjustments have to take place for a female student athlete to be successful in all environments, surveys have been evaluated for patterns, similarities and differences. Recognizing the extensive gap in research surrounding the student-athlete academic experience, I was intrigued to explore the collegiate athlete experience and how academia plays a role in their mindsets, perceptions of themselves, their college experiences, priorities, and the relationships they cultivate. ~ 40 ~ PROCRASTINATION AND THE COLLEGE STUDENT’S MIND Lauren Newman with Professor Michelle Neely (Adisor) Department of English University of Colorado Colorado Springs Procrastination is a common habit among college students. One typical area this is seen in is essay writing. Procrastination is frowned upon, for the most part. However, how much damage does procrastination actually do? Can there be any benefits to students? This study is to provide insight on the actual causes of procrastination, the effects, and the mental strain students experience. This information is collected from anonymous survey answers from students across three different college campuses. ~ 41 ~ ~ 41 ~ FOSTERING INTRINSIC GROWTH IN THE SECONDARY ENGLISH CLASSROOM: KINDLING THE FIRES OF LANGUAGE ARTS LEARNING Joe Konik with Professor Michelle Neely (Adisor) Department of English University of Colorado Colorado Springs Literacy, as the keystone of scholastic and social success, poses an instructional challenge in public education. This project, based upon in-class observations and instructor interviews, is targeted at building upon the existing research regarding Language Arts instruction in the secondary education classroom. Contemporary research outlines pitfalls as well as strengths of traditional Language Arts instruction. The aim of this study is to determine and highlight some of the best practice methods for encouraging student growth and engagement in the Language Arts classroom. ~ 42 ~ THE EFFECT OF JOURNALING ON ACADEMIA Marybeth McBain, Shya Hess with Professor Michelle Neely (Adisor) Department of English University of Colorado Colorado Springs Our research will be looking at how journaling affects students’ learning throughout their academic careers. We will be surveying about 40 college students and 10 high school or college teachers. The survey will help us to understand if journaling, personal and educational, is positive for the learning process. Our hypothesis is that journaling will produce better comprehension and organization skills in the reading and writing process. ~ 42 ~ Oral Session II (1:00-2:00 pm) Technology, Social Media, Money Room: Centennial Hall 102 ~ FLIGHT LINE OF THE FUTURE-AUGMENTED REALITY Foster Meier, Kyle Lenz, Eric Millen, Christopher Ruiz, Jack Taute with Major Daniel White (Advisor) Department of Management United States Air Force Academy The Air Force has spent millions of dollars on high level aircraft maintenance in the past year alone. Lockheed Martin is currently developing high fidelity virtual mapping technology called 3D SME (Subject Matter Expert) that could revolutionize the way maintenance is performed on aircraft platforms. Coupled with augmented reality hardware to overlay instructions, diagnose potential problems, and document maintenance processes, Lockheed Martin has the potential to completely redesign Air Force maintenance procedures. These technologies are in preliminary stages with limited current capabilities. Our Air Force Academy team has been tasked to determine the value of pursuing this technological endeavor and to quantify exactly how this new equipment could save money for the Air Force, reduce aircraft downtime, and increase maintainer quality of life. Using our recommendations, Lockheed Martin will utilize our research as a benchmark to determine how much they will invest in continuing their 3D SME and Augmented Reality Systems development. ~ DEVELOPMENT OF MARKETING STRATEGIES FOR NEW MILITARY SOCIAL MEDIA SERVICES Adam Carr and Matthew Schuetz with Maj Abizer Tyabji (Advisor) Department of Management United States Air Force Academy, CO 80840 As the number of personal and professional social networks increase, there lies a need for a networking tool for use by military members. In an occupation where many do not feel in control of their career or ready to transition to civilian life, this social media company seeks to put military members back in charge of their lives and network. This ~ 43 ~ project examines the process by which people discover, join, and return to social networking websites. Using basic marketing principles and newer online marketing techniques, Data collected leads to the development of marketing ideas and opportunities to increase membership for the website. Final findings will likely boost membership and total awareness of these services available to military members. ~ REARRANGING DISTRIBUTION CENTERS TO ALIGN WITH THE STORES THEY SERVE Adam Carr, Ryan Kunce, Mike Leland, Connor McKown with Lt Col Tim Pettit and Capt Brian Lemay (Advisors) Department of Management United States Air Force Academy Walmart is an organization whose success is dependent largely on their logistical efficiency. Currently, Walmart is looking to improve how their distribution centers arrange pallets of dry groceries in order to reduce the labor required to stock those items in store aisles. This project focuses on the refinement of this process through the development of a computer-based tool that provides Walmart with the optimal distribution center profile based on the stores each center services. The tool allows the user to scale distribution center profiles at the national, regional, and store-specific levels. The project consists of three phases: problem definition and research, development of a user-friendly computer program, and cost/benefit analysis. Findings from this project provide insight into rearrangement of each distribution center’s layout based on potential savings from the restocking process at each of Walmart’s 3942 retail stores. _____________________ Human Factors II Professor Calkin’s Class Room: Centennial Hall 106 ~ THE EFFECTS OF PHYSICAL EXERCISE ON COGNITIVE PERFORMANCE Amanda Caudill, Frederick Dellecker, Brad Rajchel with Major Bryan A. Calkin Department of Behavioral Sciences and Leadership United States Air Force Academy Physical exhaustion often becomes a significant factor in high activity jobs. Real world examples of the effects of physical exhaustion of the visual-motor abilities can be supported through the observation of both athletes and soldiers alike. Soldiers and ~ 44 ~ athletes alike often face scenarios where their success or failure depends on their visual-motor abilities. This study will investigate how physical activity affects an individual’s ability to visually scan and find a target. The study will analyze how individual’s cognitive performance is affected by undergoing physical activity. Several variables are being manipulated to include the physical level of difficulty. Participants will undergo physical activity followed by a visual search task in which completion time will be recorded. ~ EFFECTS OF WORKLOAD ON DRIVING PERFORMANCE James Africano, Danielle Griffith, Daniel Neal, John Rush with Maj Bryan A. Calkin Department of Behavioral Sciences and Leadership United States Air Force Academy Driving is a daily activity that requires a lot from the driver. With new mobile technologies becoming available to the everyday driver, the workload is increased on an already demanding task. This study investigates driving performance when an operator maneuvers through a set driving course with varying levels of workload. Participants will be put through a closed driving course during which they will be sending a number of text messages of varying complexity. Their performance on the course will be measured through a number of variables including text response time, cones hit, and speed limit deviations. _____________________ Birds & Deer Room: Centennial Hall 186 ~ UTILIZATION OF AIRBORNE SOUND AND LIGHT COUNTERMEASURES TO REDUCE BIRDSTRIKES Carlo Mancini and Trent Grabowski with Capt Jeff Newcamp Department of Aeronautics United States Air Force Academy This cadet project sought to understand the possible utility of employing airborne countermeasures to prevent bird strikes. U.S. Airways Flight 1549, which was struck by a flock of Canada geese (Branta Canadensis) in 2009, resulting in a water landing on the Hudson River in New York, was taken as a baseline event with a negative outcome. ~ 45 ~ Position data were taken from that incident to model the audio and visual stimuli incident on the geese to design an experimental countermeasure for bird strike reduction. This effort utilized modeling of the visual environment and a ground test of a system capable of approximately mimicking the history of the engine noise and visual irradiance of the Airbus 320 involved in the accident. During testing, the response times of geese movement were recorded for the baseline Airbus stimuli and a combination of specific acoustic and visual countermeasures. The acoustic countermeasure was the addition of a proprietary Canada goose distress call. The second variable was flashing the simulated Airbus landing lights at 0.75 Hz. The testing was conducted using a ground-based system on Canada goose populations on fields at and near the Air Force Academy. This cadet-run experiment is currently underway and is scheduled to run through late April. The expected finding is that the addition of a flashing landing light and a distress call will cause the geese to respond significantly earlier than the stimuli without the countermeasures. The ability of the test to determine optimum acoustic and visual stimuli was limited by the number of wild geese flocks available to be tested. _____________________ Scheduling for Fun & Profit Room: Centennial Hall 188 ~ RESOURCE-CONSTRAINED SCHEDULING TO INCREASE TISSUE PROCESSING THROUGHPUT Eric Amos, Chancellor Johnstone, Patrick Morgan, Kevin Rossillon with Lt Col Tim Pettit, Maj Michael Chiaramonte, Capt Brian Lemay Department of Management United States Air Force As one of the leading bone and tissue processing banks in Colorado, AlloSource is committed to honoring the gift of each and every donor by maximizing the utilization of usable bone and tissue to meet hospital needs. In order to improve the overall efficiency and throughput provided by AlloSource, this study developed the AlloSource Matrix Optimization Scheduler (AMOS), a simple tool which utilizes genetic algorithm scheduling heuristics on integer programming with an intuitive user interface to optimize scheduling based on constrained resources and scheduling priorities. These resources include processing technicians, room capabilities, equipment capabilities, and donation expiration times. AMOS links the requirements into a collective schedule and then outputs the results to the user, providing a quick solution to the timely task of deriving a schedule by hand and ultimately delivering an optimized schedule for the upcoming shift. Utilization of the tool allows AlloSource to optimize their scheduling process with ~ 46 ~ sensitivity analysis providing avenues for short and long-term improvement of scheduling procedures. ~ ENHANCING RMB PRODUCTS’ PRODUCTION SCHEDULING Jacob A. Forbes, Patrick M. Harrison, Wrendy K. Rayhill, and Daniel R. Schonfeld with Capt Brian Lemay (Advisor) Department of Management United States Air Force Academy RMB Products is a premier manufacturer of highly engineered, high performance, thermoplastic solutions for demanding industrial applications. Currently, RMB Products generates a dispatch list at the beginning of each day outlining the tasks that must be completed. Given the variation and uncertainty of work times and fluctuating priorities of work orders, workers often complete tasks out of order or are unable to complete their tasks on schedule. This study examines RMB Products’ scheduling system and recommends improved work-time standards to enhance delivery performance. ~ VALIDATING EQUIPMENT INVESTORIES AND CREATING EFFICIENT SCHEDULES FOR TEST EQUIPMENT Ian Butler, Tyler Meirose, Nathan Mitchener, and Stephen Savonne with Dr. Dale Peterson (Advisor) Department of Operations Research United States Air Force Academy Presented is a project is to aid a large defense contractor manufacturer more efficiently utilize test equipment. The manufacturing process requires that materials, components, and systems be tested with specialized test equipment that replicates operational conditions. The contractor possesses test equipment of different capabilities with slightly differing specializations spread across multiple campuses around the country. Differences make it difficult to efficiently schedule testing jobs, and without an efficient schedule the company may maintain more test assets than necessary. The research team contacted 13 labs to confirm the capabilities of 44 different pieces of test equipment, creating a centralized database of these capabilities as a resource for test schedulers. Then, a forecasting program was developed to analyze test equipment usage. By analyzing usage, the sponsor will be able to: 1) Determine which test equipment (if any) should be eliminated from inventory, 2) provide data to management in regards to purchase of additional equipment, and 3) effectively execute testing operations. ~ 47 ~ _____________________ Examinations of War & Society Room: University Center 302 ~ POLARIZATION AND THE ARGENTINE DIRTY WAR IN LA HISTORIA OFICIAL AND LOS CENSORES Daniel Barbera Department of Foreign Languages United States Air Force Academy Native Argentines Luis Puenzo (b. 1946) and Luisa Valenzuela (b. 1948) used artistic license to present their points of view about Argentine society during and after the period associated with the Dirty War (1955-1983). Puenzo, an award winning film director, and Valenzuela, an acclaimed international writer, used their work to represent the complexity of the atrocities that occurred in their homeland. The violence, fear, and censorship depicted in both Puenzo’s film, La historia official, and Valenzuela’s short story, Los censores, divided the Argentine population into two groups: those who conformed and those who became oppressed. The extent of interpersonal violence and insensitivity is disturbing, but it can be understood in the context of the polarization and depersonalization in Argentine society of the day. Censorship limited the voices of the oppressed by preventing them from speaking out against the authoritarian government. Cornered by censorship and fear of kidnapping, the daily lives of Argentines were shaped and defined by those in control. The societal conflicts of the Argentines represented in La historia official and Los censores depict the proclivity of the human race to polarize society and illustrate the consequences of such polarization. ~ DISILLUSIONMENT AND LANGUAGE: PERCEPTIONS BEYOND WAR Nathaniel Hall, Drew Pineda, Alannah Staver Department of English United States Air Force Academy The following three presentations will address conventional notions of war and examine the authenticity of these perceptions. These presentations do not aim to discourage the actions of soldiers in war. Rather, they serve to elucidate repercussions of the established narrative of rationalization found in the works visited. Furthermore, the presentations offer insight for the maxims and provide, perhaps beneficial, alternative. ~ 48 ~ Cadet Hall’s presentation examines the ways in which humans perceive the idea of time and how these perceptions are challenged in the midst of war. C1C Hall posits that in preserving one’s authenticity, the conventional notions of freedom and democracy may be stripped away. By taking ideas of time and being and putting them within the context of war, Cadet Hall’s presentation seeks to find truth the “truth” about war. Cadet Pineda’s presentation explores the psychological processes and phenomena that affect societies when they are engaged in war. The presentation begins with a broad scope of psychological factors and narrows from a societal lens to a personal lens - for example, the ways in which war is justified by a nation, and then by an individual. One will find fresh insight into war, which is an entity that validates the lives of millions of people. Cadet Staver’s presentation deals with rationalization in war, and calls it into question. Cadet Staver says that the human capability to rationalize controversial behaviors and thoughts is disturbingly infinite. During war, the soldier’s ability to make sound moral decisions is frequently lost in the bedlam of mortar fire, guerilla attacks, and insurgencies, making it easy to justify even the most horrific actions. She explores some examples of rationalization through the scope of war in “The Forest of the Laughing Elephant,” by Philip Caputo, March by Geraldine Brooks, and Black Hawk Down by Mark Bowden. _____________________ Cinema Room: University Center 303A ~ TRAVERSING THE FALSE SEXUALITY AND POWER OF THE FEMALE IN POSTMODERN AMERICAN POPULAR FILM Vanessa Los with Professor Robert von Dassanowsky (Advisor) Feminist Cinema University of Colorado Colorado Springs This presentation will through close reading examine the continued traditional fetishization of the female image in post-modern American popular film of the twentyfirst century. The re-visioned stereotyping of politically correct "humanization" or "differentiation" of the female role is revealed to reflect the instability of social structures in the U.S. and the West. I will focus on a representation of films that differ in genre and production modes (The Dark Knight, Black Swan, and The Core). ~ 49 ~ ~ THE ZOMBIE IS UNTRUTH: AN EXISTENTIAL ANALYSIS OF THE ZOMBIE FILM THROUGH ASPECTS OF KIERKEGAARD, HEIDEGGER, NIETZSCHE, AND ORTEGA Y GASSET Jon Gillin University of Colorado Colorado Springs This paper seeks to answer what the dramatic rise in global production and popularity of zombie cinema suggests on a philosophical basis. It will attempt to answer this question through a representative analysis of the genre using existential philosophy’s driving force: the struggle for the individual to rise over the crowd. Kierkegaard famously stated, “there is no place, not even one most disgustingly dedicated to lust and vice, where a human being is more easily corrupted – than in the crowd.” As existentialist thought is primarily concerned with the development of the individual over and against the threatening crowd or mob culture. It serves as an ideal theoretical mechanism for analyzing and understanding archetypes that exist within the genre of zombie film. ~ ARBITRARY DEATHS: THE ANTI-TRAGEDY OF JEAN-LUC GODARD’S À bout de souffle and Vivre sa vie Sean Purcell University of Colorado Colorado Springs The tragic elements of Jean-Luc Godard’s À bout de souffle and Vivre sa vie can be seen as anti-tragic. Both À bout de souffle and Vivre sa vie appear as classically aligned tragic narratives, and while both films depict the death of the main characters, the films employ a web of inflated tragic signifiers, Brechtian techniques and a tension between Bazian long takes to disrupt the surface appearance of the films. These films fall under meta-tragedy or anti-tragedy. They both bare the signifiers of the tragic genre, but ultimately they erode the significance of the deaths in their film, questioning the whole notion of tragedy. ~ 50 ~ Poster Session II (2:00-3:30 pm) Room: Berger Hall ~1~ EFFECTS OF BARNACLE AND ALGAE GROWTH ON BOAT PERFORMANCE James E. MacAndrew, Travis C. Miller, Matthew D Underwood, Kyle A Casci, Dariel R Baker with Maj Daniel White (Advisor) Department of Management United States Air Force Academy Every year the 75 million boat owners in America pay thousands of dollars to rid their boat of algae and barnacles in order to mitigate damage, increase performance, and sustain the resale value. There are only a few processes today to help alleviate this problem; paint-based materials that have proven ineffective, a scrape technique to remove these unwanted organisms, and an acid-based chemical product application to clean the hull. The problem with these processes is that they are time consuming, expensive, and potentially harmful to the boat and environment. ShieldTec is researching a state-of-the-art product developed to provide a way for boat owners to keep their boat hulls clear and free of buildup while reducing the amount of manual labor required in the cleaning and repair process. This multifunctional polymer delivers an inexpensive, easy to use, high quality product that effectively repels organisms with its anticoagulant properties. ~2~ DISTINGUISHING MALWARE AND SOFTWARE BASED ON STRUCTURE Andre Brito, Ramon Vilanueva United States Air Force Academy Malware is an enormous threat to the internet and the multitude of computer systems attached to it. The rate of new malware has been rapidly increasing in recent years due to the practice of making malware variants. A malware variant is new malware created by making slight modifications that are enough to bypass malware detection software. Due to the fact that new malware is frequently a variant of other known malware, the structures of malware are similar. Just like non-malicious software, the creation of new malware contains commonalties of the previous malware design. This paper aims to ~ 51 ~ distinguish between the structure of malware and software. By accomplishing this, one should be able to identify the properties of an unknown piece of software to find if it is malicious. This paper will try to find the commonalities and differences malware contains compared to legitimate software using various techniques such as byte frequencies and system call comparisons. ~3~ WEIGH AND WIN: RETENTION ANALYSIS Nolan Bet, Steven Chon, Kyle Cranston, Jack Lepird, Megan Muniz with Dr. Nathan Wozny, Capt Brian Lemay (Advisors) Department of Management United States Air Force Academy Weigh and Win is a free community program, funded by Kaiser Permanente and administered by incentaHEALTH. It is a public weight loss program for the state of Colorado. The goal of this project is to improve the program’s quality by helping incentaHEALTH better understand the Weigh and Win participants. On the administrative data available covering almost 13,000 participants over the course of a year and a half, we used statistical and econometric methods to analyze factors that led to people staying in or quitting the program. These methods yielded key demographics of participants who stuck with the program versus participants who did not. For example, at the 99% significance level, participants in the 18-24 age group are 34% likely to not be retained, while people in the 50-54 age group are 22% likely to be retained. Other characteristics found to be significant include BMI, gender, enrollment date, and whether or not the participants were on a team. This study also identifies groups of participants with low retention and allows Weigh and Win to focus their efforts accordingly as well as identify promising practices that might improve future retention. ~4~ DNA EXTRACTION METHODS AND DNA BARCODING OF LICHENIZED FUNGI Trenton G. Grabowski with Katherine L. Bates (Advisor) Department of Biology United States Air Force Academy Lichens are abundant and present almost anywhere, composed of roughly 13,500 accepted species that account for nearly 18% of fungal species worldwide (Kelly et al., 2011). The study of lichens is very broad and shallow because accurate identification requires taxonomic expertise involving nontrivial tasks; even though lichenized fungi represent one of the best known components of the diversity of fungi, some samples ~ 52 ~ lack certain characteristics that easily distinguish between various species of lichens (Kelly et al., 2011). Therefore, we developed a protocol to molecularly identify lichen species using DNA barcoding. Various DNA extraction techniques, to include both homogenization buffer and nuclei lysis solution, were tested using both archived and fresh samples to determine the most effective protocol. Next, we optimized the PCR protocol using both plant and fungal primers for high quality sequencing. The goal of this project is to create an easy-to-follow protocol that other investigators can use to easily identify lichen species, and ultimately catalog the lichen diversity of the Pikes Peak region. ~5~ ENERGETIC COMPOSITE FILLER SEQUESTRATION IN GEL-BASED NANO-COMPOSITES David Alvarado with Lt Col Royce Beal (Advisor) Department of Chemistry United States Air Force Academy Recent experimentation with the use of cryogels to contain energetic materials has shown promise as a method of controlling morphology and combustion/detonation characteristics. Hydrazinium nitroformate (HNF), monomethyl hydrazinium nitroformate (MMHNF), and similar energetic materials show significant promise in solid rocket propulsion but have suffered stability and rheology problems. Here a variety of sol-gel to cryogel techniques and combinations were employed to prepare a series of resorcinolformaldehyde (RF) and hydroxyl-terminated polybutadiene (HTPB) cryogels infiltrated with inert nano-crystalline salts like ammonium sulfate to pave the way for infusion with HNF. Experimentation with layering optimization and the sol-gel to cryogel method while using scanning electron microscopy for verification set the foundation for the synthesis of a cross-linked nitrocellulose (NC) matrix infused with the nano-crystalline HNF. Analysis of the various propellant-infused cryogels included elevated temperature aging and thermal/combustion property assessments. ~ 53 ~ ~6~ FINDING COMMON GROUND IN DIFFERENT APPROACHES TO THE GEOMETRICAL FOUNDATIONSL OF ELECTROMAGNETISM Scott Alsid With Lt Col Mario Serna (Advisor) Department of Physics United States Air Force Academy Traditionally, gauge theory has focused on the matter fields and gauge fields as the main object of study and how they transform. While alternative approaches to studying gauge theory have emphasized a geometric perspective, there are several different camps, each with their own tools. This paper seeks a common ground with two camps, those who use an embedding for their geometry to obtain a metric and those who use tetrads to replace the gauge fields. This paper shows that the ideas expressed in each of these lines of thought are pursuing the same lines of thought, just with different mathematical techniques and notation. Examples for electric and magnetic fields, U(1), gauge theory, are shown for each camp with visualizations from the embedding camp. ~7~ PREPARATION OF FULVENE-BASED DYE SENSITIZED SOLAR CELLS Michael Duff with Hongshan He, Gary J. Balaich, and Endrit Shurdha (Advisors) Department of Chemistry United States Air Force Academy The title work describes the synthesis, characterization and testing of donor-π-acceptor fulvenes for possible applications in dye sensitized solar cells (DSSC). The focus of this research is on the use of a synthetic strategy for the selective and stepwise attachment of appropriate donor and acceptor groups around 1,3,6-tri(substituted) fulvene core. A model synthesis was carried out using 1,3-diphenylcyclopentadiene and 9.10bromoanthracenecarboxaldehyde to give the 6-(bromanthracene) substituted fulvene. Sonogashira coupling with 4-ethynylbenzaldehyde and subsequent attachment of the cyanoacetic acid group gave the desired substitutiedfulven dye. This methodology produced analogues of a series of new fulvene dyes that were tested using photophysical measurements (UV-VIS and fluorescence) as well as DSSC efficiency. ~ 54 ~ ~8~ THE IMPACT OF CHILD PORNOGRAPHY ANALYSIS PERFORMED AT THE US ARMY CRIMINAL INVESTIGATIONS LABORATOR (USACIL) ON LEGAL PROCEEDINGS Victoria Wyler with Dr. Candice Bridge, Mr. Levi White (USACIL, Forest Park, GA), Capt Hanna Yang (Advisors) Department of Law United States Air Force Academy Criminal investigations of child pornography currently require digital examiners to review all evidence submitted by the government for forensic review. This process is not only time consuming, but also difficult despite the capabilities of modern technology. Specifically, in child pornography investigations involving military service members, limitations must be created to maintain timely case output notwithstanding the increasing amount of data that examiners must review. This study analyzed sixty-six U.S. Army case files dating from 2009 to 2011, all of which involved child pornography. The amount of child pornography on each exhibit was collected to determine if a correlation between the amount of child pornography and the overall judicial outcome of a case existed. Findings show that the amount of child pornography found on exhibits did not highlight a direct relationship with the judicial outcome of an individual case; therefore, supporting the need for new more timely methods of examination. ~9~ INJUSTICE IN THE BALANCE: AN ANALYSIS OF JUDICIAL CORRUPTION IN LIBERIA AND SOLUTIONS FOR IMPROVING TRANSPARENCY AND EFFICIENCY Nathanael I. Tousley with Capt Hanna Yang (Advisor) Department of Law United States Air Force Academy This project’s purpose is to provide the government of the City of Monrovia with recommendations for addressing the crippling effects of judicial corruption and inefficiency in Monrovia, Liberia and surrounding areas. These findings and recommendations will be compiled in the book, “Rule of Law’s Evolution in Liberia” slated for publication in 2015. All of the research for this project was accomplished firsthand through case studies and interviews in and around Monrovia. Interviews were conducted with the Liberian Anti-Corruption Commission, the Liberian National Police Division of Professional Standards, the National Democratic Institute, the Carter Center, the Monrovia Central Prison, and several judges and prosecutors at levels from the ~ 55 ~ magisterial court to the circuit court. The recommendations from this research include utilizing and training community leaders to serve as judges for misdemeanor offenses and offering legal education classes to the public to improve their trust and understanding of the legal system. ~ 10 ~ A COMPARISON OF THE MAJOR POLPHENOLIC AND ANTI-OXIDANT CHARACTERISTICS OF Cola nitida ssp. Alba, Cola nitida ssp. Rubra, and Cola acuminate Evan K. Shawler, Steve Freund, Alexa Gingras, Taeyoung Lee, Joseph Sarette with Dr. Barry W. Hicks (Advisor) Department of Chemistry United States Air Force Academy In sub-Saharan Africa, Cola nuts are commonly eaten as stimulants because of their high caffeine content, but interestingly, the food has also become an integral component of almost every social function. Most cultural ceremonies cannot be completed without the distribution of Cola nuts. In addition to the caffeine, the nuts contain elevated levels of polyphenols and anti-oxidants, which can provide various health benefits. The compounds of interest are being extracted from ground Cola nut then analyzed by HPLC-MS, GC, and GC/MS. In this study, three sub-species of Cola are being examined, Cola nitida ssp. alba, Cola nitida ssp. rubra, and Cola acuminata. By identifying and quantifying the compounds of interest, they can be compared between the three sub-species. The gas chromatograph of the hexanes extract from Cola acuminata displayed no noticeable peaks. The results from HPLC have indicated poor separation between compounds of interest. Future work will include percent weight of caffeine, theobromine, and catechin and a comparative analysis that delineates their quantitative contents. ~ 11 ~ POROSITY AND PHASE TRANSITION CHARACTERIZATION OF ALUMINA XERO AND AEROGELS Hilary M. Bowen With Dr. Margaret H. Rakowsky (Advisor) Department of Chemistry United States Air Force Academy Xero and aerogels were prepared through sol-gel synthesis using AlCl3•6H2O and a 50/50 % vol solution of ethanol/H2O as solvent. The porosities of the gels--macropore, ~ 56 ~ mesopore, or micropore-- were characterized by scanning electron microscopy. Fractional phase compositions were analyzed by powder x-ray diffraction. Differing α/γ phase compositions were found to be directly related to the temperatures at which the sol-gels underwent calcination. The ability to control pore size in alumina sol-gels offers potential synthetic methods for the production of size selective catalysts, catalytic supports, and scaffolding for biosensors, while the ability to control the alpha/gamma phase selectivity in these gels offers a way to directly influence their specific material properties. ~ 12 ~ PREPARATION OF POLYAMIDES FROM COMMON DIAMINES AND PENDANTVARIABLE DIACYL HALIDES Ryan P. Aceves with Drs. John D. Peak, Timm A. Knoerzer, Scott Iacono (Advisors) Department of Chemistry United States Air Force Academy Considerable interest exists within synthetic polymer chemistry for the development of novel materials for a variety of applications including microelectronics, catalysts, energy systems, environmental applications, nanofabrication, and sensors. In our laboratory, we are interested in developing functionalized polymeric materials constructed around readily exploitable reactive handles that afford on-demand orthogonal manipulation. To achieve this goal, we present a tripodal monomer that when polymerized using Schotten-Baumann reaction conditions , leaves latent functionalities that possess a cross-linking capacity or more attractively for the incorporation of modular functionalization that can be tuned to service the desired application. The tripodal monomer with reactive acyl halide groups will be subjected to a two -layer solvent system with a diamine to create an alternating copolymer system. Polymerization efficiency is demonstrated via GPC and MALDI -TOF mass spectrometry. The diamine may also be a tripodal monomer to allow for increased abundance of reactive handles and further manipulation servicing the desired application. ~ 13 ~ THE EFFECT OF PASSWORD CONSTRAINTS ON MEMORABILITY Carl Morgan and Kenneth Sample United States Air Force Academy Because being unable to remember passwords well leads to compromising actions such as writing down or reusing passwords, the memorability of a password is an important aspect to password security. A survey was conducted to find how different ~ 57 ~ lengths and constraints on passwords affect memorability, and also to gather information regarding how often passwords are reused or written down. The study found that long passwords without constraints are similar in memorability to all short passwords, and that long passwords with constraints hinder memorability. ~ 14 ~ DEVELOPMENT OF TAILORABLE PFCB ARYL EITHER POLYMERS FOR HIGH PERFORMANCE APPLICATIONS Olawale Lawal with Sharon C. Kettwich, Scott T. Iacono (Advisors) United States Air Force Academy Semifluorinated perfluorcyclobutyl (PFCB) aryl ether polymers are a class of processable, step growth polymers that are easily tailorable for a multitude of high performance applications. Recently, it has been shown that PFCB aryl ether oligomers possessing reactive aryl trifluorovinyl ethers (TFVE) undergo block copolymerization with polyols/polybisphenols employing an aryloxylation methodology. In this work, PFCB-segmented silicone polymers were prepared in high yield via the step-growth addition polymerization of discreetly sized PFCB prepolymers and carbinol silicones. These hybrid fluorosilicone polymer systems displayed a high degree of thermal stability as determined by the decomposition temperatures found using TGA analysis and were structurally characterized using 1H/19F NMR and GPC analysis. Thermal analysis by DSC proved the polymers were entirely amorphous with tunable glass transition temperatures. Film preparation employing spin casting demonstrated excellent solution processability. Work continues to post-functionalize these polymers for development of proton exchange membranes for fuel cell applications. ~ 58 ~ ~ 15 ~ METASTABLE ALUMINIZED PERFLUOROPOLYETHER EPOXY BASED COMPOSITES Eryn K. Avjian with Hannah A. Miller, Sharon C. Kettwich, Scott T. Iacono (Advisors) United States Air Force Academy Epoxy resins are used widely as host matrix materials for blending particulates in order to tailor bulk properties for commercial markets such as coatings, adhesives, and laminates. Fomblins,a class of oligometic perfluoropolyethers, are used to coat nanometer-sized aluminum (nAl) particles to prepare a core-shell motif. The Fomblin/nAl particle formulations at varying weight percents were entrained in poly(ethylene glycol)-based epoxy resins. The discussion of these systems will include processing methodology and bulk mechanical/thermal properties of these systems. ~ 16 ~ NEOCORTICAL NEURONAL MORPHOLOGY IN THE INFANT GIRAFFE (GIRAFFA CAMELOPARDALIS TIPPELSKIRCHI) AND INFANT ELEPHANT (LOXODONTA AFRICANA) Larua Lee with Bob Jacobs (Advisor) Colorado College Although neocortical neuronal morphology has been recently documented in the adult African elephant (Loxodonta africana; Jacobs et al., 2011) and giraffe (Giraffa camelopardalis tippelskirchi; Jacobs et al., submitted), nothing is known about cortical architecture in the infant elephant or giraffe. Thus, the current study explores motor and visual cortices in the infant giraffe, and frontal, motor, and visual cortices in the infant elephant. Cortical neurons stained with a modified Golgi technique (N = 153) were quantified on a Neurolucida system (MBF Bioscience, Inc.). Qualitatively, neurons in the infant giraffe appeared similar to those in adult giraffes and were consistent with those observed in rodent and primate species. In contrast, neurons in the infant elephant, like those in adult elephants, varied greatly from the “typical” (i.e., like the murid rodent or primate) cortical morphology, particular with regards to pyramidal neurons. In general, projection neurons were observed in both species (i.e., pyramidal neurons, n = 46; magnopyramidal neurons, n = 5; horizontal pyramidal neurons, n = 5), with a much greater variety documented in the elephant (e.g., flattened pyramidal neurons, n = 10; multipolar neurons, n = 18; inverted pyramidal neurons, n = 3; crab-like neuron, n = 1). Although local circuit neurons in both species (i.e., interneurons, n = 40; neurogliaform neurons, n = 4) resembled those observed in other eutherian mammals, ~ 59 ~ these were usually spiny, which contrasts with their adult, aspiny-equivalents. Quantitatively, infant elephant neurons were less spiny but had longer dendritic branches than those in the giraffe, perhaps because of widely diverging apical dendrites in elephant projection neurons. In conclusion, cortical neuromorphology in the infant giraffe is consistent with what has been observed in other cetartiodactyls, whereas infant and adult elephant morphology appears to deviate considerably from what is commonly seen in other mammals. ~ 17 ~ RECRUITMENT MECHANISM OF HISTONE DEACETYLASE 3 TO REPLICAITON SITES AND ROLE FOR HISTONE DEACETYLASES IN DNA DAMAGE RESPONSE Hannah Wellman Colorado College Histone Deacetylases (HDACs) are enzymes that modulate chromatin structure, mediate transcriptional repression and contribute to DNA damage repair. HDAC inhibitor compounds show promise as chemotherapeutic cancer treatments through disruption of chromatin structure and interference with cancer cell replication mechanisms. HDACs are divided into four classes according to function, location and expression patterns. Class I HDACs include HDACs 1, 2 and 3, and are recruited to DNA replication sites through chromatin-associated proteins. HDACs 1 and 2 are known to be recruited to DNA via the Chromatin Associated Factor 1 (CAF-1) complex, but the recruitment mechanism of HDAC3 is not well understood. To investigate the recruitment complex associated with HDAC3, we performed co-immunoprecipitation experiments. We found that unlike HDACs 1 and 2, HDAC3 is not associated with CAF-1. Instead, HDAC3 interacts with RbAp48 and msin3a, transcriptional corepressor proteins. These results suggest that the HDAC3 recruitment complex is different than those of other Class I HDAC molecules. Previous research has confirmed the role of HDACs in DNA damage repair. Inhibition of HDAC function induces chromatin defects, indicating disrupted DNA repair pathways. To investigate the mechanism of this involvement, we first performed immunofluorescent analysis in HDAC-inhibited and control cells. Cells with inhibited HDAC activity displayed a significant increase in gamma-H2AX foci, an indicator of double-strand breaks. Further immunofluorescent experiments showed increased CAF-1 foci, indicators of the Chromatin Associated Factor 1 protein complex, in HDAC-inhibited cells with induced DNA damage. This provides a possible mechanism through which HDACs are recruited to damage sites. A more thorough understanding of both HDAC recruitment mechanisms and role in DNA damage repair could lead to better, more targeted cancer chemotherapies. ~ 60 ~ ~ 18 ~ THE DEVELOPMENT RELATIONSHIP BETWEEN THEORY OF MIND AND THE UNDERSTANDING OF EMOTIONS IN CHILDREN WITH HIGH FUNCTIONING AUTISM Elizabeth Leona Crow Burrus Colorado College Prior research has indicated that Theory of Mind (ToM) and the understanding of selfconscious emotions are impaired in children who have High Functioning Autism (HFA) and Asperger’s Syndrome (AS) relative to typically developing children, which may account for the disparity between these groups in social competency. This study examined both capacities in neurotypical (N = 22) and HFA/AS (N = 20) male children between the ages of 6.5 and 13 years old. Participants were asked to define both basic emotions (happy, sad) and complex emotions (pride, embarrassment, shame, guilt) and to provide a personal narrative describing a time when they experienced each emotion. Participants also completed a modified version of the Strange Stories ToM Task (Happe, 1994). As predicted, ToM and understanding of self-conscious emotions were significantly lower in both the younger age group relative to the older age group and the HFA/AS group relative to neurotypical group. ToM mediated the relationship between group and age and the understanding of self-conscious emotions, suggesting that ToM is the mechanism through which self-conscious emotions come to be understood as one develops. Implications for future research and treating the social deficits of HFA/AS children based on these findings were discussed. ~ 19 ~ LiDAR AS A TOOL FOR ARCHEOLOGICAL RESEARCH Jim Schindling, George Rinell University of Colorado Colorado Springs Traditional archaeological site surveys are expensive endeavors with cost increasing in proportion to the difficulty of access to a given site and the remoteness of the site. Surveys are also somewhat hit-or-miss in their ability to identify all features at a given site. Field work is further complicated by wooded and mountainous terrain where the establishment of a survey grid can be very difficult. In these types of environments, even the identification of surface features can be difficult due to low brush, debris and other floor cover. In addition to the physical constraints working against traditional archaeological techniques, there have been recently created regulations that limit the amount of disturbance that can be caused to areas of potential historic or cultural significance. This research investigates the use of Light Detection and Ranging ~ 61 ~ (LiDAR), sometimes called Airborne Laser Scanning (ALS), as a technology that can be implemented to address the above mentioned obstacles to archaeological research. ~ 20 ~ FISSION YEAST Rad26ATRIP AND Ase1PPRC1 PARTICIPATE IN A CHECKPOINTRESPONSE TO INTERPHASE MICROTUBULE DAMAGE Molly Cameron, Raul Perez, Chris Richey with Professor Tom Wolkow (Advisor) Department of Biology University of Colorado Colorado Springs Fission yeast Rad26ATRIP is a regulatory subunit of Rad3ATR that occupies central positions within DNA damage response pathways. This interphase microtubule damage checkpoint operates in the cytoplasm, since mutation of a nuclear export sequence in Rad26ATRIP disrupts this microtubule damage response without affecting the DNA damage response. The nes-rad26ATRIP allele also compromises cellular morphology and mini-chromosome stability, suggesting that this checkpoint safeguards morphology and chromosome number. ~ 21 ~ EXPRESSION OF DETOXIFICAITON GENES IN DROSOPHILA METTLERI REARED ON NATIVE AND NONNATIVE CACTUS HOSTS Kim Hoang with Professor Jeremy Bono (Advisor) Department of Biology University of Colorado Colorado Springs Adaptation is the force that creates the incredible range of diversity found on earth: organisms adapt to their unique environment and become unique themselves. While the concept of adaptation has been understood for some time, the molecular basis that underlies adaptive traits is still poorly understood. Here we investigate gene expression in Drosophila mettleri, a fruit fly that utilizes soil soaked in rotting cactus (its host plant) for breeding, to elucidate the molecular mechanisms involved in its host-plant adaptation. Specifically, we are comparing the level of detoxification gene expression between two populations of D. mettleri reared on different cactus hosts, each of which has a unique chemical composition, to identify genes contributing to the fly’s ability to survive in the toxic environment of its host plant. ~ 62 ~ ~ 22 ~ PERCEIVED DISCRIMINATION, STEREOTYPE THREAT, AND OPTIMISM AMONG NATIVE AMERICAN ADOLESCENTS Jamie Jaramillo with Professor Zena R. Mello (Advisor) Department of Psychology University of Colorado Colorado Springs In this study, perceived racial/ethnic discrimination, stereotype threat, and optimism were examined in a sample of 129 Native American adolescents aged 14-19. The first research question under investigation is, how much do Native American adolescents report experiencing racial/ethnic group discrimination and stereotype threat? The second research question to be explored is, how does racial/ethnic discrimination and stereotype threat predict optimism among Native American adolescents? Participants were recruited from several public high schools and an academic summer program. A self-report survey measuring the variables of interest was completed by participants. ~ 23 ~ BRAIN RESPONSES TO EMOTIONAL WORDS IN PEOPLE WITH DIFFERENT ADULT ATTACHMENT STYLES Laura M. Lathrop with Professor Michael A. Kisley (Advisor) Department of Psychology University of Colorado Colorado Springs Attachment theory provides a foundation for understanding adult relationships. Research indicates that attachment styles established in childhood continue to influence individuals into adulthood, affecting the way adults interpret and respond to emotional and interpersonal information and events. These patterns of responding can be maladaptive and negatively affect interpersonal relationships. Part of this influence comes from differences in the way anxious and avoidant individuals pay attention to emotional information. Theory indicates that anxious individuals are overly concerned about their relationships and fear abandonment. They tend to be hyper-vigilant toward attachment-related threats, giving extra attention to relationship cues. Theory proposes that avoidant individuals maintain distance in intimate relationships and tend to suppress attention toward attachment-related information, meaning they tend to ignore relationship cues. Until recently, the allocation of attention to emotional information has been studied using indirect, behavioral methods. In contrast, this project looks directly at the manner in which individuals with anxious and avoidant attachment styles allocate their attention to emotional information by measuring brainwave responses to positive ~ 63 ~ and negative, general and attachment-related emotional words presented in a background of neutral words. This project is novel in that emotional information is divided into general and relationship-oriented themes, and also into the positive and negative aspects within those themes; additionally, it includes a measure of brain responses to track attention allocation. Combining these elements promises to allow a more nuanced understanding of attention allocation in people with differing attachment styles. ~ 24 ~ MALE ACCESSOR-GLAND PROTEIN TRANSCIPTS PRESENT IN THE REPRODUCTIVE TRACTS OF MATED DROSOPHILIA MOJAVENSIS FEMALES MAY HAVE IMPORTANT IMPLICATIONS FOR UNDERSTANDING POST MATING MOLECULAR INTERACTIONS Alexis Mascarenas with Professors Eugenia Olesnicky-Killian, Professor Jeremy M. Bono (Advisors) Department of Biology University of Colorado Colorado Springs In internally fertilizing organisms, post-mating molecular interactions between the sexes occur within the female reproductive tracts that are necessary for successful reproduction. In many species, male accessory gland proteins (ACPs) are known to interact with components of the female reproductive tract, triggering physiological processes in mated females including immune response, sperm transfer and female receptivity. A previous study has shown that Acp mRNA transcripts are detected in the reproductive tracts of Drosophila mojavensis females after copulation. Although it is know that ACP proteins are transferred from males to females, the discovery that mRNAs are as well is novel, and may have important implications for understanding post mating molecular interactions. Once transferred, these mRNAs are not rapidly degraded and persist in the females for at least six hours, indicating they may serve a functional purpose. Here, I will use in situ hybridization to investigate where in the lower reproductive tract the Acp transcripts are present following copulation. ~ 64 ~ ~ 25 ~ RAPID DETERMINATION OF A PANEL OF NEUROTRANSMITTERS IN HUMAN URINE USING CAPILLARY ELECTROPHORESIS Kathryn a. Prescott, Char Brecevic, Morgan Pinto with Professor David J. Weiss (Advisor) Department of Chemistry University of Colorado Colorado Springs Serotonin (5-HT), dopamine (DA), norepinephrine (NE), and epinephrine (E) have been linked to many neurological conditions. However, the relationship between these neurotransmitter concentrations and diseases is not well understood. A technique that offers rapid, non-invasive quantitative analysis of neurotransmitters could aid physicians and researchers when studying neurological diseases. Capillary electrophoresis (CE) is an effective method for small, charged molecules, like neurotransmitters. CE yields high peak efficiencies, and is environmentally friendly due to its minimal use of organic solvents. Presented here is a rapid method to identify and quantitate four neurotransmitters simultaneously in human urine. Solid-phase extraction (SPE) was used for sample clean-up and preconcentration. UV/Vis detection was utilized for analysis of samples with elution times of approximately 10 minutes. ~ 26 ~ EXPRESSION ANALYSES INDICATE A ROLE FOR THE GENE shep IN THE DEVELOPMENT OF THE CENTRAL NERVOUS SYSTEM, MIDLINE, AND TRACHEA Ismail E. Sola with Professor Eugenia Olesnicky-Killian (Advisor) Department of Biology University of Colorado Colorado Springs Shep encodes a RNA binding protein conserved across multiple species including humans (RBMS1,2,3), zebrafish (RBMS 1a,3), mouse (RBMS 2,3), as well as many other species. Analysis of shep mRNA distribution in Drosophila embryos indicate cell specific expression starting at stage 11 in the central nervous system midline cells and later, at stage 15 in the ventral nerve cord, brain, and trachea. Interestingly, tracheal expression of shep indicates a role for shep in regulating cell movement or migration in the embryo. We hypothesize shep is involved in cell migration pathways in both the nervous system and the trachea. ~ 65 ~ ~ 27 ~ THE UTILITY OF BEHAVIORAL INTERVENTIONS AMONG INDIVIDUALS WITH DOWN’S SYNDROME AND DEMENTIA Sandra Garcia, Mary Steers with Professor Leilani Feliciano (Advisor) CU Aging Center University of Colorado Colorado Springs Individuals with developmental disabilities are living longer than ever before, with an average life expectancy near 60 years old (Torr & Davis, 2007). As the population of aging adults with developmental disabilities increases, health care professionals must be prepared to manage the consequences of the cognitive impairments that develop in later adulthood, including dementia. Individuals with Down’s syndrome (DS) have an increased risk of Alzheimer’s disease (AD), with prevalence rates of 50-75% by the age of 65 (Torr & Davis, 2007). As individuals with DS endure the stages of a progressive dementia, they may exhibit disruptive behaviors, such as agitation (e.g., verbally and/or physically nonaggressive behaviors, verbally and/or physically aggressive behaviors), which can negatively affect the individuals quality of life and increase caregiver burden. Interventions to reduce agitation among individuals with developmental disabilities have been effective, and many have been successfully applied to people with AD. Unfortunately, there is little research focusing on whether these interventions will have the same efficacy with individuals with both DS and AD. The present study will utilize single case design methodology to evaluate the utility of function-based behavioral interventions for reducing agitation in individuals with comorbid DS and AD. Additionally, interventions will aim to increase quality of life among these individuals and decrease caregiver burden. Implications for behavior management in this population will be discussed. ~ 28 ~ SELF-EFFICACY AND OTHER INFLUENCES IN STEM CAREERS; INVESTIGATING DIFFERENCES AMONG UNDERREPRESENTED STUDENTS Audrey K. Szarka with Peter D. Marle, Lisa L. Decker, Dave H. Khaliqi (Advisors) CSTEME/PEPES University of Colorado Colorado Springs A shortage exists among college graduates entering STEM (science, technology, engineering and mathematics) fields, thereby threatening United States’ STEM competitiveness. Bandura (1977) presumed self-efficacy largely impacts performance and thus future career choices. Consequently, CSTEME (Center for STEM Education) programs endeavor to increase self-efficacy in STEM and inspire students to pursue ~ 66 ~ STEM careers. Students (N = 600; grades 5-12) completed pre-surveys prior to CSTEME workshop participation, and qualitative responses to the question “Please list 3 jobs you would like to have when you grow up” were coded (i.e., Science, Technology, Engineering, Mathematics, and non-STEM careers) for analysis. Results showed various levels of predictability between self-efficacy and attitude variables and interest in STEM and non-STEM careers. Further, differences between school level, first-generation status, gender, and race/ethnicity were explored. Understanding variable predictions of STEM career matriculation may lead to effective STEM program practices thereby boosting the number of college graduates pursuing STEM careers. ~ 29 ~ IMPACT OF BACTERIAL INFECTION ON EXPRESSION OF TWO PATHWAYS OF THE DROSOPHILA INNATE IMMUNE SYSTEM, TOLL AND IMD, FOLLOWING EXPOSURE TO ENVIRONMENTAL STRESS Kim Cox, Kathryn Gray, Martha Bales University of Colorado Colorado Springs The maintenance and deployment of the innate immune system is assumed to be energetically costly to organisms, by using resources that could otherwise have been allocated to other life history processes. To search for potential trade-offs, we will investigate the impacts of environmental stress on the operation of two pathways that produce antimicrobial peptides as part of the innate immune response in Drosophila melanogaster. Flies will be assigned to one of three stress treatments (cold shock, heat shock, or starvation), or to a non-stressed control group. Flies from each of these four stress treatments will be placed in one of three infection treatment groups: uninfected, infected with Gram-positive bacteria (Staphylococcus aureus) or infected with Gramnegative bacteria (Escherichia coli). We will use quantitative real-time PCR to quantify expression of the antimicrobial peptides Diptericin, produced by the Imd pathway in response to Gram-negative infection, and Defensin, produced by the Toll pathway in response to Gram-positive infection. With this experiment we attempt to discover any trade-offs within the Toll and Imd pathways of the innate immune system resulting from environmental stress. ~ 67 ~ ~ 30 ~ CHILDREN’S PERCEPTIONS OF WRITING BEFORE ENTERING KINDERGARTEN Lena Liedtke with Professor Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs Educators are well aware that writing does not just start when a child enters a Kindergarten classroom. The theory of emergent writing would suggest that writing skills emerge as children develop. Through exposure at home, pre-school and in everyday surroundings children have usually developed opinions about writing. This study presents how 5 and 6-year-olds view writing before entering the Kindergarten classroom and formal education. I hope to give the children interviewed a sense of power and ownership in their educational process, give parents an idea of where their child is in regards to thoughts on writing, and serve as a reminder to educators of the beginnings – where children are coming from. ~ 31 ~ LOS SAN PATRICIOS: VICTIMS OR SOLDIERS OF FORTUNE IN THE WAR WITH MEXICO Kimberly Sweetwood University of Colorado Colorado Springs Los San Patricios, an assembly of deserters from the United States army who turned and fought for the Mexican army during the Mexican-American War, were captured after the Battle of Churubusco and tried by general court martial for desertion. What caused these men to desert? By analyzing contemporary military documents, contemporary accounts of the Mexican-American War, along with immigration, naturalization, and census data, and current scholarship this investigation will demonstrate that los San Patricios did not desert the United States army strictly because of religious discrimination, but were in fact influenced by the active recruitment of the Mexican army promising social and economic opportunities in addition to religious freedom. ~ 68 ~ ~ 32 ~ A COMPARISON OF THREE MODIFICATIONS OF THE VAN DER WAALS EQUATION OF STATE Dustin DiSalle with Professor James Eberhart (Advisor) Department of Chemistry and Biochemistry University of Colorado Colorado Springs The equation of state (EOS) of a fluid not only provides the dependence of fluid density on temperature and pressure, but it is also a useful thermodynamic tool that enables the prediction of certain physical properties from the measurement of other properties. A popular starting point for the creation of new EsOS is the modification of the famous, two-parameter, van der Waals EOS. One approach is to replace one or both of the parameters with a function of temperature. In this study we have explored three different temperature-dependent attractive parameters inspired by the work of Berthelot, Prausnitz, and Soave. In each case a third parameter is introduced in the resulting EOS. The three parameters are evaluated using the experimental values of the critical temperature and pressure, as well as the Riedel factor (which is the tangent to the vapor pressure curve at the critical point). The accuracy of these three EsOS (as well as the original van der Waals EOS) are evaluated via the prediction of three different properties for simple fluids, namely, the second virial coefficient at the critical temperature, the Boyle temperature, and the zero-pressure inversion temperature. On the basis of these predictions, all three modified EsOS provide significant improvement over the original van der Waals equation. The best of the three modifications is the one that employs the temperature-dependent attractive term suggested by the hybrid of the van der Waals and the Berthelot EsOS. ~ 33 ~ THE WRITING PROCESSES OF CREATIVE WRITERS IN CREATIVE AND ACADEMIC WRITING Ashley Stuard with Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs This study investigated whether creative writers, at all levels of the writing process, approach writing differently when they write creatively as opposed to academically. Through three interviews with creative writers who are also college students, this study analyzed which processes writers used, which processes were emphasized, and how similar or different the processes were between the two genres of writing. Additionally, creative writers were asked about their conceptions of writing in order to discern whether or not their conceptions informed their writing processes. ~ 69 ~ ~ 34 ~ SERMON PREPARATION Kacee Saxe with Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs This study reports on interviews of adults who have participated in Christian discourse via Sermons. Transcripts were analyzed for emotional and rhetorical components of the sermon preparation. ~ 35 ~ WRITING INSTRUCTION IN CHARTER SCHOOLS Amy Renfro with Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs The purpose of this study is to understand writing instruction in charter schools, using a local charter school as a case study. It is the aim of the study to learn details such as the amount of time spent on writing instruction, the effects of teaching cursive, and what factors are most likely to promote students’ success in writing. ~ 36 ~ THE LIMITS OF STRUCTURE AND THE FREEDOM OF EMOTION: THE CREATIVE WRITING PROCESS OF LYRICISTS Matthew Denning with Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs Music is a universal pleasure that encompasses almost every aspect of our daily lives. There is an inherent feature within an individual’s particular choice of music that connects the pleasure received from the music to the actual medium of art. As lyrics often represent this feature, there are questions that arise when addressing this unique ~ 70 ~ form of individual creativity. As both external factors and internal factors dictate the composition of music, the writing process of lyrics begins to illuminate this unique writing process. This project explores the question: How is the writing process of lyrics implemented in a musical group and how are the external and internal factors relevant to the composition of lyrics? Through the analysis of written artifacts and interviews, this study addresses these questions as they are relevant to lyricists. ~ 37 ~ UNDERSTANDING TEACHING METHODS OF FOREIGN LANGUAGES Alecia M. Johnson with Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs “Understanding Teaching Methods of Foreign Languages” is a study about the effects on students’ learning of English through different teaching methods. The goal of this study is to identify the benefits and disadvantages of different methods in order to find commonalities that could help professors enhance their curriculum and students better their study habits. The information for this study is gathered through interviews with college/university students and professors as well as through survey results from SurveyMonkey.com. ~ 38 ~ USING FLOW CYTOMETRY TO ANALYZE NEUTRAL LIPIDS IN CHLAMYDOMONAS REINHARDTII Polina Reynolds with Professor Sandy Berry-Lowe (Advisor) Department of Biology University of Colorado Colorado Springs Due to the rising costs of fossil fuels, renewable biofuels from a variety of sources are being explored as viable alternatives. Chlamydomonas reinhardtii is a unicellular green alga that is a useful model organism for studying biofuel production because it can be cultivated in many environments, variegated mutants are available, and it is amenable to analysis by flow cytometry. Previous research has suggested that abiotic stress, such as nitrogen starvation and various chemical compounds, can induce increased neutral lipid (triaglycerol/TAG) accumulation in lipid droplets. Oxamate is a pyruvate analog, known to be an inhibitor of lactate dehydrogenase and glycolate dehydrogenase. Due to ~ 71 ~ its potential to inhibit glycolysis, oxamate may lead to metabolism disruption that is sufficient to cause lipid accumulation. Wild type, wall-less, and light sensitive algae strains were exposed to nitrogen deficient media, varying oxamate levels, and combinations of the two for 4-48 hours before being assessed for lipid content. Nile Red, a lipophilic stain, was used to fluorescently stain intracellular lipids and flow cytometry was used for high throughput detection and screening of live individual cells. We thank Bill Townend and UCCS Biofrontiers for use of the flow cytometer. ~ 39 ~ DIFFERENCES BETWEEN CREATIVE AND ACADEMIC WRITING: WRITER’S ATTITUDES, TECHNIQUES, AND BEHAVIORS David King with Michelle Neely (Advisor) Department of English University of Colorado Colorado Springs As the title suggests, this study attempts to determine some of the differences between writers’ approaches to creative and academic writing. In order to achieve this, the study includes two sets of data. The first set consists of several in-depth interviews with people who write in both creative and academic spheres. The second set analyses creative and academic writing samples taken from the same group. These data sets are then cross examined to find correlations between the interview responses and writing choices of the participants. ~ 40 ~ EXPLORING PARAMETERS IN MICROWAVE ASSISTED ORGANIC SYNTHESIS (MAOS) Christ Butler, Brayden Hamill with Allen M. Schoffstall (Advisor) Department of Chemistry and Biochemistry University of Colorado Colorado Springs Microwave (MW) assisted organic synthesis (MAOS) requires use of scientific microwave instrumentation. This equipment is much more sophisticated than the $200 MW ovens sold in department stores. The instrumentation provides for reaction vessels that withstand pressures in excess of 50 bar (725 psi). The high pressure vessels allow heating of volatile solutions to temperatures of 100-200°C, higher than can be achieved at ordinary reflux temperatures. Our project is a study to maximize the quality and quantity of product formed in an ordered three-pronged MAOS reaction involving SN2 ~ 72 ~ substitution, decarboxylation and then cycloaddition using click chemistry. 2’Bromoacetophenone undergoes the SN2 reaction with sodium azide while decarboxylation of 3-phenyl-2-propynoic acid occurs releasing CO2 and exposing a terminal alkyne. The resulting organic azide and alkyne condense in the presence of copper sulfate pentahydrate and sodium ascorbate (Vitamin C) in a 1:1 tert-butyl alcohol/water solution. Our theme is varying parameters to achieve the best outcome. ~ 41 ~ CADET PACKAGE INSTANT NOTIFICATION SYSTEM (CPINS) Benjamin Kram, Nathan Lebens, Max Robertson, Julie Ahn, Bamboo Hewitt with Maj Daniel White, Lt Col Charles Murnieks (Advisors) Department of Management United States Air Force Academy CPINS is an information technology project that will reduce service and wait times in the cadet mailroom by sending instant notifications via email to cadets when they have a package available for pick-up. CPINS will minimize the need for the use of the current yellow slip notification system which requires personal travel to the mailroom by creating faster package lock box turnaround and freeing up more lock boxes for use each day. The current lock box availability is only 150-200 out of 500 lock boxes with approximately 500-1000 new packages arriving each day. Minimizing the use of yellow slips will reduce the time it takes for package clerks to deliver packages by an estimated 2 hours per day and save the service window clear 1 hour per day, saving the USPS $10,560 per academic year by reducing the number of packages that must be handed out, and save cadets approximately 1600 hours per academic year excluding weekends. ~ 42 ~ NEUMIMIC Chad Moore, Melissa Fuerst, Haley Holcombe, Colton Beuchel, Ryan Wallway, Gage Owens, Marcela Leano, Daniel Neal, Ralph Tatum, Nico Gigante with Capt Ryan Silva (Advisor) Department of Electrical and Computer Engineering United States Air Force Academy More than 7 million Americans have survived a stroke, yet only 10% of stroke victims recover completely. The remaining 90% of stroke victims are left to live altered lifestyles. The purpose of NeuMimic is to give stroke victims a cost effective in-home rehabilitation system to help re-establish the connection between the arm and the brain. NeuMimic allows the therapist to enter select exercises for the patient to complete at home via Microsoft Kinect. The system will provide the services of a portable, on-call ~ 73 ~ Occupational Therapist by delivering instant feedback to the patient and tracking his/her improvement. NeuMimic is a revolutionary rehabilitation system that will significantly increase the quality of life and reduce the recovery time for stroke victims. ~ 43 ~ WEST NILE VIRUS IN YELLOWSTONE NATIONAL PARK Chris Jordan, Keith Blount University of Colorado Colorado Springs United States Air Force Academy The purpose of this research was to identify resident mosquitoes and to determine the prevalence of West Nile Virus in Yellowstone National Park. Such data can then be used to develop a disease-risk assessment for parking personnel and visitors. Mosquitoes were collected from various locations in Yellowstone National Park (YNP) during June and July 2011. Mosquitoes (N=641) were identified through comparison of morphological features using dichotomous keys and identification texts. The mosquitoes were then pooled by area and date, and tested for potential infectivity of West Nile virus (WNV) using the VecTest assay. All assays returned negative results for WNV. The results of this research project indicate that WNV, if present, is uncommon at YNP. Thus, the current disease risk for YNP from WNV would be very low. ~ 74 ~ Oral Session III (3:00-4:00 pm) Autonomous Systems Room: Centennial Hall 102 ~ DYNAMICS MODELING OF ELECTROMAGNETIC FORMATION FLIGHT Andrew R. Hilton United States Air Force Academy Electromagnetic formation flight (EMFF) is a method of holding satellite arrays in a formation without the use of propellant. A formation of smaller satellites that work together can be more effective and cheaper than one larger satellite performing a similar mission. EMFF will enable the United States Air Force to develop flexible, robust space systems by splitting different systems and payloads into modules that link together on orbit and fly in a formation. Such systems will reduce the complexity of design as well as increase the ability to respond to unforeseen occurrences during mission operations. The concept of EMFF relies on the fact that the spacecraft in the formation are flying relative to each other and uses attraction and repulsion forces to actuate the system. The research presented here analyzes these relative forces while detailing the development and verification of a Simulink dynamics model for an electromagnetic formation flight project at the Space Systems Laboratory. Biot-Savart’s law is used to characterize the magnetic fields from each coil and model the resulting forces and torques. The model uses finite element analysis to compute the forces and torques exchanged between different segments on the two coils. The simulation has been accurate in modeling the forces and torques induced by resonant coils as a result of their relative position and orientation thereby allowing future researchers to develop and test formation-flying control algorithms before using the valuable on-orbit time allocated for hardware testing. ~ 75 ~ ~ ANDROID-BASED PAYLOAD FOR AUTONOMOUS UNMANNED AERIAL SYSTEMS Craig Stan, Joshua Sprang, Brennan Sweeney, Austin Sena with Capt Michael Tanner (Advisor) Department of Electrical and Computer Engineering United States Air Force Academy Unmanned Aerial Systems’ payloads play a major role in the strategic employment of air power; however, currently payload systems are created from custom (read: expensive) parts which can be difficult to integrate while meeting ever-changing mission requirements. Our project focuses on creating a payload with commercial-off-the-shelf parts to improve the cost, time-to-market, and performance of the payload. Furthermore, the payload provides capabilities on-board image processing, autopilot integration, GPS waypoint system, and target tracking/detection. ~ ROBOTIC PACE SETTER Mark Richardson with Dr. John Wood (Advisor) Department of Engineering Mechanics United States Air Force Academy, Colorado Springs This project will design, develop and test an autonomous robotic device to act as a pace setter to aid athletes in running US Air Force Academy Physical Fitness Test (PFT) and Aerobic Fitness Test (AFT). The PFT consists of a 600 yard run at an approximate pace of 14 miles per hour around an indoor track and the AFT consists of a 1.5 mile run on an outdoor track. An autonomous flying multicopter was selected as the robotic platform to accomplish the design task. Multicopters can be programmed and flown using an ArduPilotMega (APM) autopilot with open source Mission Planner (MP) software. This software allows for setting waypoints on a map that the multicopter will follow using GPS and altitude sensors. An indoor navigating system must be developed to replicate the GPS information that is unavailable indoors using sensors such as sonars, optical flow sensors or even a triangulation system. ~ 76 ~ ____________________ Social Ties Room: Centennial Hall 106 ~ COLORBLINDNESS REFLECTED IN THE LAW Norbert Grover with Dean Peter Braza (Advisor) College of Letters, Arts, and Sciences Dean’s Office University of Colorado Colorado Springs Sociologist Donald Black once theorized that the law is primarily a mechanism that perpetuates and preserves the influence of those who hold the societal reins of power. In support of this, Black quotes Rousseau when he states that The universal spirit of laws, in all countries, is to favor the strong in opposition to the weak, and to assist those who have possessions against those who have none. This inconveniency is inevitable, and without exception. (p. 12) I will argue that contemporary “colorblind” legislation facilitates societal stratification along racial lines, ensuring the existence of the status quo with regard to education, employment and politics. Academics like Cheryl I. Harris (2003) propose that throughout American history and into the present day, “colorblind” legislation or laws that covertly marginalize people of color continue to act as barriers to an even distribution of societal resources. For example, legislation such as SB 1070 targets and demonizes the existence of the Mexican immigrant just as the Wagner Act disproportionately benefitted the poor White in contrast to his Black counterpart during the inception of a growing American middle class. In the context of Donald Black’s theory, law operates as a social control mechanism. Accordingly, often the dictates of law make certain behavior normative. I intend to argue that certain “colorblind” initiatives in law create a legal marginalization of people along racial lines. Furthermore, I will argue that contemporary “colorblind” law is just as detrimental to the Black in America as Jim Crow was to the Black of the 1950s and 60s. ~ 77 ~ ~ OF WAR AND WORDS: THE SPANISH CIVIL WAR REPRESENTED THROUGH HER SONGS Molly Engle with Dr. Sheri Long (Advisor) Department of Foreign Languages United States Air Force Academy This research project presents the notable role of music as a unifier, morale builder, and inspirational motivator for soldiers of both sides during the Spanish Civil War (19361939). An analysis of the lyrics and melodies from this time period focuses on three major aspects of the Spanish Civil War. They mark the war’s progress, the ideologies of the Nationalists and Republicans, and the authors and their motives. A comparison of 13 well-known war songs reveals the distinct motivation behind each side. The Nationalists incorporated simple, solid themes that created a unified voice behind the idea that they were fighting to preserve: their traditional Spanish identity that revered loyalty to the Catholic Church, the family, and the state. On the contrary, since the Republicans varied greatly in political ideologies (i.e. anarchists, communists, socialists) within their own group, their songs express more universal calls to liberty and equality that were values necessary in establishing a new, free Democratic Spain. ~ PAINT, PANTIES, AND PIN-UPS: THE SOCIAL AND PSYCHOLOGICAL EFFECTS OF THE SEXUALIZATION OF THE AMERICAN PIN-UP Maria Tobin with Barbara Headle (Advisor) Department of History University of Colorado Colorado Springs Throughout the 1930s, 40s and 50s, American pop-culture in conjunction with the social media transformed the female figure into a highly sexualized image while ensuring its ability to maintain the innocence and vulnerability expected of the female gender. The establishment of the “All American Pin-Up Girl” revolutionized the pop-culture and social media of the era as it was combined with patriotic slogans to support the war effort both at home and overseas. By focusing on the works of great Pin-Up artists including Earl Moran, Zoë Mozert, and Art Frahm, as well as secondary arguments presented by modern American and art historians alike, the American Pin-Up became an artistic phenomenon that affected American society socially, psychologically and aesthetically by establishing itself in modern United States history as an iconic style of American art. ~ 78 ~ _____________________ Understanding Our Environment Room: Centennial Hall 186 ~ EFFECTS ON WATER QUALITY IN SOUTHEASTERN UNITED STATES Matthew Cahalan University of Colorado Colorado Springs Agriculture, urban land use, and climatic events have increased the fluxes of nutrients, sediments, and different organic/inorganic chemicals into surface waters and ground waters. As a result, watersheds are under various levels of environmental pressures due to diminished water quality. Specifically, the increased nitrogen flux to rivers, estuaries, and coastal waters has enhanced phytoplankton blooms as part of the overall eutrophication process. Discharge rates of nitrate in streams are not commonly matched to different types of land use or to field application rates. To promote the longterm sustainability of natural and managed watersheds and to develop successful remediation strategies, fundamental processes that control water quality on a watershed scale must be investigated. This research is designed to understand nitrogen fluxes in watersheds with different land uses, and then eventually engineer cost effective solutions to reduce and mitigate the nutrient footprint of businesses, towns, and municipalities. ~ AN EXPERIMENT WITH LOW COST, SUSTAINABLE DRINKING WATER PURIFICATION TECHNIQUES Andrew Munoz Department of Civil Engineering United States Air Force Academy The lack of clean drinking water is the fifth leading cause of death for adults and the second leading cause of death for children under the age of five (World Health Organization, 2002). This project studied low cost, sustainable means of purifying drinking water for use in developing countries. Specifically, we studied how grain size effects biological sand filtration since very small sand grains (<0.7mm) that have been proven effective for use in biosand water filters can be difficult to locate and isolate in developing countries. Additionally, we studied how important post-filtration ultraviolet [solar] disinfection is because, while recommended by the Center for Affordable Water and Sanitation Technology (CAWST), it is not always practiced in developing countries due to the additional effort and time required. Escherichia coli (e. coli) and turbidity reduction were two measures of effectiveness used in this study. Two biosand water ~ 79 ~ filters were built: a control filter (d10 = 0.19, UC = 2.1) in accordance with CAWST specifications that limits grain size to 0.7mm and an experimental filter (d10 = 0.80, UC = 1.5) with grain sizes up to 2.0mm. Jenkins, et al. (2011) concluded that smaller grained biosand filters significantly or near significantly improved bacteria removal from water; however our experimental filter’s grain size more drastically differed from the control than theirs and we compared our results to international standards to determine if larger grains could still meet them. CAWST recommends disinfection after biosand filtration. Wilson (2010) found that water filtration using a roughing filter can help increase the effectiveness of solar disinfection; our filters employed a biological layer which used predation and natural death in addition to mechanical trapping to potentially enhance the reduction of bacteria and turbidity. The data show e. coli and turbidity removal characteristics of both filters were not significantly different, and both met international drinking water standards. Additionally, ultraviolet disinfection eliminated all e. coli from both filters’ effluent. The results of this project indicate the grain size requirements in biosand water filters may be relaxed, and disinfection may not be required to meet international drinking water standards. ~ THE SYNTHESIS OF A NOVEL ORGANOMETALLIC LIGAND FOR ALKLYTHIOL REMOVAL IN NATURAL GAS Bethany Neighbors with Dr. Renee Henry (Advisor) Department of Chemistry and Biochemistry University of Colorado Colorado Springs Abstract: A common method of H2S and alkylthiol removal from raw natural gas is by the amine process. These processes require large volumes of volatile amines, elevated pressures and temperatures. An organometallic complex would replace the need to use large volumes of amines, elevated pressure and temperatures currently used to remove acid gas contaminants. The primary research goal seeks to produce a structure suitable for removal of H2S and alkylthiols. The ligand of interest is N, N’, N’’, N’’’ 1,4,8,11 tetrakis (N-isopropyl acetamide) tetraazacyclotetradecane (TIAC) which is formed from the reaction between 2-bromo-N-isopropyl acetamide and 1,4,8,11tetraazacyclotetradecane (cyclam). The methods of synthesis and characterization of TIAC will be presented. ~ 80 ~ _____________________ Systems Optimization Room: Centennial Hall 188 ~ IMPROVING THE PERFORMANCE OF INTERNATIONAL SHIPPING INFORMATION SYSTEMS Brian Hwang, Royd Lim, Paul Gaski, and Jon Jones with Lt Col Tim Pettit, Capt Brian Lemay, and Capt John Miller (Advisors) Department of Management United States Air Force Academy FedEx is an international shipping company that processes more than 3.4 million packages daily. Due to frequent changes in regulations and requirements of each country, FedEx has supplemented their existing systems that process individual packages. This study focuses on FedEx’s international shipping operations and provides FedEx with a detailed map of their system interfaces and associated traffic from system calls. By analyzing the thousands of system calls that occur each day, the study aims to understand how each of FedEx’s systems interacts and to identify opportunities to reduce system complexity. Additionally, by identifying various bottlenecks that occur between systems, FedEx is able to identify and address specific issues and reevaluate their system workloads in order to avoid system failures and manual intervention. As FedEx increases its international shipping volume and continues to grow, analysis of its error-prone systems is crucial for preventing critical and costly errors in the future. With this study’s results and recommendations, FedEx is able to more efficiently manage and operate its international shipment system. ~ OPTIMIZING RETAILER COUPON DISTRIBUTION Shayne Boyd, Colby Browning, Raymond Hill, Austin Howard, and Katy Dunkelberger with Capt Brian Lemay, Capt Peter Furseth and Capt Nick Mastronardi (Advisors) Departments of Management, Economics & Geosciences United States Air Force Academy In January 2012, Sports Authority started utilizing the services of Catalina Marketing Corporation. Catalina Marketing Corporation distributes coupons at grocery and convenience store cash registers based on consumer spending patterns and specifications given by Sports Authority. The goal of this study is to maximize Sports Authority’s gross margin by determining the optimal markets in which to distribute Catalina coupons during a national campaign. To accomplish this, the benefits and costs of coupon redemption in a particular market must be balanced. Regression ~ 81 ~ analysis on coupon redemption forecasts the benefits of distributing coupons in a particular market. A linear program then balances forecasted benefits with operating and promotional budgets to determine in which markets to print coupons. With these results, Sports Authority can more effectively utilize Catalina’s services to increase profits. ~ INVENTORY ANALYSIS FOR WAREHOUSE COST REDUCTIONS Maria Phillips, Hunter Hamer, Stephen Welling, Kathleen Vardell with Lt Col Tim Pettit, Maj Justin Hill, and Capt Brian Lemay (Advisors) Department of Management United States Air Force Academy Currently, the Black Hills Corporation’s warehouse in Pueblo, CO orders material based on intuitively chosen reorder points and quantities. They also face an inventory problem because delays with buyers in Rapid City, SD and numerous vendors create long and unpredictable lead times. Using EOQ (Economic Order Quantity) and Reorder Point equations to determine these reorder levels and amounts can minimize the cost of inventory for black Hills Corporation. This project calculates these reorder points and quantities using past data. A total cost comparison between their current process and the proposed one will be presented. Results will be integrated with PeopleSoft software currently employed by the customer. In addition, process improvements to reduce lead time variability in the ordering systems will also be presented. This project concludes with results of potential inventory costs savings under various services levels. _____________________ Hitchcock & Shakespeare University Center 302 ~ THE WIVES OF VILLAINS: COMPLICITY IN EVIL Canyon Chambers with Advised Andrea Van Nort (Advisor) Department of English and Fine Arts United States Air Force Academy Shakespeare criticism of two of Shakespeare’s most notorious villains, Iago and Macbeth, typically focuses on Iago as a single agent and Macbeth as driven by Lady Macbeth. It is true that, most often, when mentioning Macbeth, the architect of his earliest murder, Lady Macbeth, comes quickly to mind. With regard to Iago, however, ~ 82 ~ his wife, Emilia, barely earns a side note in critical queries. This paper will attempt to rectify the imbalance that exists with regard to these two key adjuvant characters. I propose to examine the roles of Emilia and Lady Macbeth as active agents in their husbands’ machinations, one unwitting, one emphatically intentionally. In doing so, I hope to challenge common perceptions and provide them both some well-deserved credit. ~ HACKING HITCHCOCK: A PRESENTATION ON THE ‘TRINITY’ OF HIS FILMS Andrew H. Drescher with Professor Robert Von Dassanowsky (Advisor) Department of Film University of Colorado Colorado Springs This presentation offers a theory on the legendary Alfred Hitchcock: his focus on the elderly, the female and their binding to the dogmatic “MacGuffin”-- a plot device designed to motivate the psychological emergence of the central characters. By analyzing three films, I will show how the "MacGuffin” also defines the woman as a reductive device serving the narrative's development of male identity while the elderly are used as metaphors for entrapment in archaic social norms. _____________________ Disharmonic Spirituality Room: University Center 303A ~ AN EXPLORATION OF NARRATIVE THROUGH BINAURAL SOUNDSCAPE Britton Ciampa with Dr. Glen Whitehead (Advisor) Department of Visual and Performing Arts/Music University of Colorado Colorado Springs This project is a collaboration between Dr. Glen Whitehead and Britton Ciamps to create a concept recording exploring and reinventing the idea of the narrative within improvised music. With Ciampa on a range of percussion instruments and Whitehead with several trumpets and trumpet like instruments, this project will employ specialized inter-spatial binaural recording techniques. These techniques, in conjunction with the specific elements of the narrative (revolving around the rabies virus), combine to create a holistic and innovative new recording and listening experience. This recording will aim to tell the story of a dog infected by the rabies virus. This is both a literal and figurative ~ 83 ~ example of disharmonic variation, societal dislocation, and our internal response systems to environmental change, personal and cultural disruption. A binaural recording allows the listener to experience recorded sound in such a relation to their own spatial sense that they feel as though they’re able to pinpoint the actual location of the sound source, even though the sound exists only in the headphones. This phenomenon has also been described as 3D stereo sound (2D stereo sound means simply hearing sound either from the left or right, while binaural 3D stereo adds the new dimension of ‘front’ and ‘back’ to sound.) ~ MEME – AN ORIGINAL MUSIC COMPOSITION Brent Wollman with Glen Whitehead (Advisor) Department of Visual and Performing Arts/Music University of Colorado Colorado Springs This piece was inspired by a short documentary video I saw a long time ago that described a parasitic organism that uses biomechanical mechanisms to modify the neurological structure of its host to the extent of dramatically altering its behavior. This particular parasite caused an ant to climb up a blade of grass, making itself vulnerable to grazing cattle, which are the parasite’s ultimate destination. I liked the idea of natural selection harnessing the power of an idea (a pattern of neural activity) to create this remarkable relationship between two distantly related organisms. What I was trying to express here was the blossoming of an ominous and unsettlingly beautiful natural phenomenon. The ant climbs absurdly to the tip of its destruction, unknowingly at the mercy of a deceitful parasite. Despite the cruelty of this process, there is something captivating about the way the insect patiently waits, seemingly by its own will, for its own death. I wanted the unfolding of this piece to evoke feelings of a dark realization gilded with vicarious wonderment. ~ KIERKEGAARDIAN DIALECTIC IN MODERNIST CLOTHING: GOD, EXISTENCE AND MUSIC IN SCHOENBERG’S OPERA MOSES UND ARON Jonathan Gillin with Glen Whitehead (Advisor) Department of Visual and Performing Arts/Music University of Colorado Colorado Springs Arnold Schoenberg’s opera Moses and Aron is work that is imbued with philosophy and theology. This work speaks volumes about Schoenberg’s own relationship to the Divine as well as his view of his musical development. Moses in the opera has a direct encounter with God and is consequently defined by this experience. He knows the ~ 84 ~ purpose of his existence through the transparent interaction he has had with God and is thereby a distinct self, separated from the crowd and called by God. For Schoenberg, the self is identified through the separation between the transcendent and immanent. This separation, and consequent definition of self are common themes in the opera. It is also the central theme in Kierkegaard’s philosophy. Kierkegaard believed that the development of the self was defined in a dialect leading from the immanent to the transcendent. In Kierkegaard’s thought the ability for an individual to become a self rests in his ability to move from the most basic experience of life, the aesthetic, through the ethical and finally, to a relationship experience with God. It is only here, in an encounter with God, that Kierkegaard believed a man could truly become a self. The parallel between Schoenberg’s opera and Kierkegaard’s philosophy of becoming a self through the transparent God relationship is obvious. This paper seeks to explore the relationship between the writings of Kierkegaard and Schoenberg’s philosophy of the individual’s relationship to the Divine as manifest in Moses und Aron. ~ 85 ~
© Copyright 2026 Paperzz