M OLTOX® is the world’s leading manufacturer of prepared Minimal Glucose Agars for use in the Salmonella mutagenicity assay (Ames Assay) and observes the highest standards in the production and packaging of prepared media. MOLTOX® prepared bacteriological media includes nutrient, selective and enrichment media used in industrial, environmental and molecular microbiology. MOLTOX® media are manufactured with the best materials: • A.C.S. “Certified” or better chemicals are employed. Impurities in commodity grade material can effect the performance of many media. • Petri dishes are highest quality polystyrene. Dishes stack well and exhibit excellent optical clarity and dimensional uniformity. • Only Type II borosilicate glass, PETG, natural polypropylene or polycarbonate containers with PTFE or white rubber closure liners are used. All containers are noncontaminating. • Solid media are formulated with industry standard Difco (BBL) Bacto and BD Agars. The MOLTOX® production facility is equipped with modern manufacturing equipment including: • O n-Line Engineering™ plate pouring machines • Systec™ media preparators • Automated liquid pumping assemblies MOLTOX® will manufacture media formulated and packaged to your specifications. Please contact us if you need media with non-standard sugars, special salts or buffers, mixtures of antibiotics, chemically defined amino acid compositions or a particular compound of interest. We will be happy to supply you with a quote corresponding to your Custom Media requirement. • Each lot of media is tested for sterility and its ability to support the growth of the appropriate microorganism. • All media are accompanied by GLP-compliant formulation and QC certificates. CONTENTS PG # Distributors 2 Ordering 3 Terms & Conditions 3 Plated & Bottled Media 4 Multi-Section Plates 15 Reagents/Buffers 16 MOLTOX Distributors ® EUROPE Trinova Biochem GmbH www.trinova.de INDIA Krishgen Biosystems www.krishgen.com Puerto rico Instrumed www.instrumed.net SOUTH KOREA Woo Jung BSC, Inc. www.woojungbsc.co.kr china Shanghai Bioplus Biotech Col, Ltd. www.bioplus-biotech.com brazil Interlab www.interlabdist.com.br north america Brand-Nu Laboratories www.brandnu.com Fisher Scientific www.fishersci.com VWR Scientific www.vwrsp.com 2 MOLTOX® Bacteriological Media To Place an Order: TERMS Net 30. Freight prepaid. Packing charges apply to dry and blue ice shipments. Payment in USD only; drafts drawn against US banks. Please contact our office for wire transfer instructions. Expenses pursuant to international monetary transactions are the responsibility of the buyer. Additional shipping and processing costs may apply. RETURN POLICY Items sent to you due to our error are immediately returnable at our expense. Items found to be unsatisfactory will be credited to your account or replaced free of charge. Shipments damaged in transit should be retained and reported within 7 days of receipt to the Customer Service Department at 1.800.536.7232. Spoiled or damaged products will be replaced or a credit issued. Your satisfaction is guaranteed. GENERAL CONDITIONS OF SALES AND WARRANTY Call M-F 8:30am- 5pm (EST) US/CAN: 828.264.9099 or 800.536.7232 INTL: 001.828.264.9099 fax US/CAN/INTL: 828.264.0103 EMAIL SALES: [email protected] INFO: [email protected] customer service [email protected] www.MOLTOX.com Molecular Toxicology, Inc. PO Box 1189 Boone NC 28607 USA Products purchased directly from Molecular Toxicology, Inc (MOLTOX®) are shipped in accordance with the specifics of your purchase order. It is the responsibility of the customer to determine the suitability of the product or products for their application: MOLTOX® products are sold for research purposes only. MOLTOX.com 800.536.7232 3 Plated & Bottled Media PRODUCT SIZE PART # 2X YT Broth 1 L/bottle 26-597 Rich growth media used for cultivating recombinant strains of Escherichia coli – also used in culture of E. coli strains for bacteriophage propagation. Ampicillin Master Plates 5 plates/sleeve 21-201 Minimal Glucose Agar with excess histidine and biotin plus ampicillin. For use with pKM101 containing Ames Salmonella strains. Bacmid Selection Agar 30 ml/plate; 10/sleeve 21-40S125 Used for isolation and selection of recombinant organisms containing bacmid DNA (a baculovirus shuttle vector). Bile Esculin Azide Agar 20 ml/plate; 10/sleeve For the selective isolation and differentiation of group D streptococci. 51-40S006 Bismuth Sulfite Agar 20 ml/plate; 10/sleeve 51-40S007 A highly selective medium used for isolating Salmonella spp., particularly Salmonella typhi, from food and clinical specimens. Conforms with specifications of The United States Pharmacopeia (USP). Blood Agar 20 ml/plate; 10/sleeve 51-40S005 Used for the isolation, cultivation and detection of hemolytic activity of streptococci and other fastidious microorganisms. Brain Heart Infusion Agar 20 ml/plate; 20/sleeve 21-40S62 General-purpose medium suitable for the cultivation of a wide variety of organism types, including many bacteria, yeasts and molds. Brain Heart Infusion Agar w/ 5% Sheeps Blood General-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. The addition of sheep’s blood facilitates the growth of fastidious fungal specimens. 20 ml/plate; 20/sleeve 21-40S85 Brilliant Green Agar 20 ml/plate; 10/sleeve For the isolation of Salmonella other than S. typhi. 51-40S015 Casein Agar Plates 20 ml/plate; 10/sleeve 21-40S189 Used in qualitative procedures for the differentiation of Nocardia species. Part of a set of Nocardia Differentiation Media (Casein Agar, Tyrosine Agar, Xanthine Agar, & Starch Agar). Cetrimide Agar 20 ml/plate; 10/sleeve 51-40S014 Also known as Pseudosel Agar. Used for the selective isolation and identification of Pseudomonas aeruginosa. Conforms with specifications of The United States Pharmacopeia (USP). Chapman Stone Agar 20 ml/plate; 10/sleeve 51-40S041 For isolating and differentiating staphylococci based on gelatinase activity and mannitol fermentation. Columbia Agar (w/o Sheep’s Blood) 20 ml/plate; 10/sleeve 21-40S111 General-purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms. Dextrose Starch Agar 20 ml/plate; 10/sleeve 51-40S021 Used for cultivating cultures of Neisseria gonorrhoeae and other fastidious microorganisms. 5 plates/sleeve 21-199 EC TRI PCTM Plates For phenotypic confirmation of WP2 strains. Tests for tryptophan auxotrophy and pKM101. 4 MOLTOX® Bacteriological Media PRODUCT SIZE PART # EE Broth Mossel 100 ml/bottle 26-554 Also known as Enterobacteriaceae Enrichment Broth. Used for selectively enriching and detecting Enterobacteriaceae. Should be used as an enrichment broth, followed by a selective medium. Enterococcosel Agar 20 ml/plate; 20/sleeve Used for the rapid, selective detection and enumeration of enterococci. 21-40S83 Eosin Methylene Blue (EMB) Agar, Modified 20 ml/plate; 20/sleeve 21-40S87 A slightly selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric bacilli. Esculin Agar Plates 20 ml/plate; 20/sleeve 21-40S193 A differential medium for demonstrating esculin hydrolysis by various microorganisms. Fluid Thioglycollate Broth Used for the sterility testing of biologics and for the cultivation of anaerobes, aerobes and microaerophiles. 200 ml/bottle 26-606 1 L/bottle 51-40S051 10 ml/tube; 10 or 100/box 22-133 Haemophilus Test Medium 500 ml/bottle 26-616 For use in dilution antimicrobial susceptibility testing of Haemophilus influenza. Hektoen Enteric Agar 20 ml/plate; 10/sleeve 51-40S008 A moderately selective medium for the isolation and cultivation of gram-negative enteric microorganisms, especially Shigella. Histidine/Biotin Master Plates 10 plates/sleeve 21-203 Minimal Glucose Agar with excess histidine and biotin. For culture of his- Salmonella strains Histidine/Biotin Top Agar (0.05mM) For use with S. typhimurium strains in the Ames assay. 100 ml/bottle 300 ml/bottle 500 ml/bottle 26-503.1 26-503.3 26-503.5 Hypoxanthine Agar 20 ml/plate; 20/sleeve 21-40S190 Used in qualitative procedures for the differentiation of Nocardia species. Part of a set of Nocardia Differentiation Media (Casein Agar, Tyrosine Agar, Xanthine Agar, & Starch Agar). KF Streptococcus Agar w/ TTC Used for isolating fecal streptococci. 20 ml/plate; 10/sleeve 21-40S82 Lactobacilli MRS Media Used for the isolation, enumeration and cultivation of Lactobacillus species. Plates 20 ml/plate; 10/sleeve 51-40S020 Broth 100 ml/bottle 26-619 Broth 500 ml/bottle 51-40S071 LB Agar (Lennox) 20 ml/plate; 10/sleeve 51-40S075 Used for maintaining and cultivating recombinant strains of Escherichia coli. LB Agar (Miller) 20 ml/plate; 10/sleeve 21-40S102 Used for maintaining and propagating Escherichia coli in molecular microbiology procedures. LB Broth (Miller) 1 L/bottle 26-598 Used for maintaining and propagating Escherichia coli in molecular microbiology procedures. www.MOLTOX.com 800.536.7232 5 MOLTOX® LB Agar Matrix X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 6 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 60 60 20 20 20 20 20 20 20 60 60 60 60 60 25 25 60 20 20 25 100 50 100 100 119 80 12.5 25 25 12.5 150 25 12.5 12.5 12.5 12.5 12.5 100 25 100 100 25 100 12.5 75 34 34 25 X 12.5 150 50 25 100 12.5 50 100 12.5 100 13.6 34 34 25 25 25 1 0.5 20 34 100 50 MOLTOX® Bacteriological Media 10 10 10 10 Rifamp. Gent. Tetra. Glucose Puro. Trimeth. Glycerol Zeocin Spect. Hygro. Chlor. X-Gal IPTG Carben. Amp. Kan. Lennox X X X X X X X Antibiotic/Additive (Concentration μg/mL) Fill size per plate (mL) Miller LB Type Part # 21-40S30 21-40S31 21-40S96 21-40S97.140 21-40S98.140 21-40S99 21-40S102.140 21-40S115 21-40S116.140 21-40S117 21-40S118.140 21-40S119.140 21-40S120 21-40S121 21-40S122 21-40S123 21-40S124 21-40S130 21-40S134 21-40S136 21-40S140 21-40S141 21-40S143 21-40S148 21-40S149 21-40S150 21-40S151 21-40S152 21-40S153 21-40S155 21-40S157 21-40S158 21-40S159 21-40S160 21-40S165 21-40S166 21-40S167 21-40S168.140 21-40S169 MOLTOX® LB Agar Matrix (cont.) X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X 20 20 20 20 20 30 30 30 30 30 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 12.5 100 80 12.5 150 150 25 80 100 100 50 100 100 99 80 X 75 50 5 5 100 15 15 40 5 15 12.5 100 100 50 75 25 12.5 25 25 75 75 25 100 12.5 25 25 15 10 5 40 35 20 100 100 20 20 20 5 20 100 0.5 5 5 20 100 35 100 35 100 20 20 Rifamp. Gent. Tetra. Glucose Puro. Trimeth. Glycerol Zeocin Spect. Hygro. Chlor. X-Gal IPTG Carben. Amp. Kan. Lennox Antibiotic/Additive (Concentration μg/mL) Fill size per plate (mL) Miller LB Type Part # 21-40S175 21-40S179 21-40S182 21-40S184 21-40S188 21-40S194 21-40S195 21-40S196 21-40S197 21-40S198 21-40S205 21-40S219 21-40S220 21-40S221 21-40S222 21-40S223 21-40S224 21-40S226.140 21-40S227.140 21-40S228.140 21-40S229.140 21-40S230 21-40S231.140 21-40S233 21-40S237.140 21-40S238.140 21-40S240.140 21-40S246.140 21-40S247.140 21-40S248.140 21-40S249.140 21-40S250.140 21-40S251.140 21-40S252.140 21-40S253.140 21-40S254.140 21-40S255.140 21-40S268.140 21-40S269.140 www.MOLTOX.com 800.536.7232 7 MOLTOX® LB Agar Matrix (cont.) 20 50 35 5 5 300 30 100 40 40 40 100 5 20 20 50 MOLTOX® Bacteriological Media Rifamp. Gent. Tetra. Glucose Puro. Trimeth. Glycerol Zeocin Spect. Hygro. Chlor. X-Gal IPTG Carben. Amp. Kan. Lennox X X X X X X X X X X 20 20 20 20 20 20 20 20 20 20 20 20 20 X X X 8 Antibiotic/Additive (Concentration μg/mL) Fill size per plate (mL) Miller LB Type Part # 21-40S270 21-40S280 21-40S282 51-40S037 51-40S075 51-40S087 51-40S088 51-40S114 51-40S115 51-40S116 51-40S117 51-40S133 51-40S136 PRODUCT SIZE PART # m-Enterococcus Agar 20 ml/plate; 10/sleeve 51-40S009 Also known as m-Azide Agar. Used for the rapid, selective detection and enumeration of enterococci. MacConkey Agar Selective and differential plating media mainly used for the detection and isolation of gram-negative organisms. Also used for isolating and differentiating lactose-fermenting/ lactose- non-fermenting gram-negative enteric bacilli. Conforms with specifications of The United States Pharmacopeia (USP). Plates 20 ml/plate; 10/sleeve 51-40S012 Broth 100 ml/bottle 26-556 Malt Extract Agar 20 ml/plate; 20/sleeve Used for isolating, cultivating and enumerating molds and yeasts. 21-40S60 Mannitol Salt Agar Used for the selective isolation and enumeration of staphylococci. Conforms with specifications of The United States Pharmacopeia (USP). 20 ml/plate; 10/sleeve 51-40S010 20 ml/plate; 20/sleeve 21-40S64 mEI Agar 60 mm plates; 10/sleeve 21-40S203 A selective culture medium used for the chromogenic detection and enumeration of enterococci in water by the single-step membrane filtration technique. Conforms with USEPA Approved Method 1600: Enterococci in Water by Membrane Filtration Using membraneEnterococcus Indosyl-β-Glucoside Agar (mEI). m Endo Agar LES Used for enumerating coliforms in water by membrane filtration. 60 mm plates; 10/sleeve 20 ml/plate; 10/sleeve 21-40S218 21-40S80 mFC Media mFC Agar and mFC Broth Base are used with Rosolic Acid in cultivation and enumerating fecal coliforms by the membrane filter technique at elevated temperatures. Plates 50 mm plates; 10/sleeve 21-40S206 Broth 10.5 ml /tube; 10 or 100/box 26-635 Middlebrook 7H10 Agar Used for the isolation and cultivation of mycobacteria. Plates 20 ml/plate; 20/sleeve Bi-plates 20/sleeve Tri-plates 20/sleeve Slant 10/box 21-40S67 21-40S285 21-40S234 22-105 Minimal Glucose Agar For use in the Ames Salmonella mutagenicity assay. Prepared as described by Maron and Ames (Mutat. Res., 113: 173, 1983); VBE, 2% glucose. Plates 30 ml/plate; 20/sleeve 21-400.2 Plates 30 ml/plate; 500/case 21-400.5 6 Well Plates 5 ml/well, 2 plates/sleeve 21-40S19 12 Well Plates 3 ml/well, 2 plates/sleeve 21-40S18 24 Well Plates1 ml/well, 0.4% glucose, 2 plates/sleeve 21-40S72 Custom Plates to your specification 21-40SXX www.MOLTOX.com 800.536.7232 9 MOLTOX® Minimal Glucose Agars 1.5 25 0.2 Oxoid No. 1 1.5 Bacto 1.5 BD Granulated 1.5 30 2 30 0.2 30 0.2 X Bacto 1.5 30 0.4 X X Noble 1.5 25 2 X X Bacto 1.5 3 2 X * * Bacto 1.5 5 2 X * * Bacto 1.5 25 2 X X Oxoid 1.5 30 2 BBL Select 1.5 25 0.25 Bacto 1.5 20 0.4 Bacto 1.5 25 0.25 BD Granulated 1.5 25 Bacto 1.5 5 Bacto 1.5 Bacto 1.5 GelRite 1.0 30 0.2 X Bacto 1.5 25 2 X Oxoid No.1 1.5 Bacto 1.5 30 2 X X 30 2 X * Bacto 1.5 28 0.4 X Bacto 1.5 20 1 X Bacto 1.5 1 0.4 X BBL Select 1.5 25 0.25 X Bacto 1.5 5 0.5 Bacto 1.5 30 2 X X Bacto 1.5 30 0.5 X X Bacto 1.5 2 0.4 X * * Bacto 1.5 5 0.4 X * * Bacto/BBL 10 Yes X No AC 2 Bacto Filt. % 1.5 30 Type Volume (ml) Plate Stacking Ring % Conc Glucose X X X X X X X X Salts Agar Comments Part # VBE Standard Ames recipe 21-400 21-40S1C VBE 50% each agar VBE 21-40S2 Davis 21-40S3 VBE 21-40S5 VBE 21-40S10 VBE 21-40S17 VBE *12 well plates 21-40S18 VBE *6 well plates 21-40S19 VBE 21-40S21 VBE 21-40S23 X VBE 21-40S26 X VBE 21-40S29 X X VBE 21-40S33 0.25 X X VBE 21-40S34 2 X * * 21-40S41 7 2 X * * VBE *6 well plates VBE *6 well plates 30 0.4 X VBE 21-40S44 X VBE 21-40S47 VBE 21-40S51 VBE 21-40S58 21-40S59 X VBE *glass Petri plate VBE X VBE 21-40S71 * VBE *24 well plates VBE 21-40S72 VBE *6 well plates VBE 21-40S258 VBE 21-40S276 VBE *24 well plates VBE *6 well plates 21-40S283 X X X X * X * X MOLTOX® Bacteriological Media * 21-40S42 21-40S65 21-40S113 21-40S262 21-40S284 PRODUCT SIZE PART # Mitus Salivarius Agar 20 ml/plate; 10/sleeve 51-40S013 For isolating Streptococcus mitis, S. salivarius and enterococci, particularly from grossly contaminated specimens. Mueller Hinton Agar 20ml/plate; 10/sleeve 51-40S055 Recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardized by the Clinical and Laboratory Standards Institute™(CLSI). Complies with requirements of the World Health Organization and is specified in the FDA Bacteriological Analytical Manual for food testing. Mueller Hinton II Broth (Cation adjusted) 500 ml/bottle 26-617 Used in quantitative procedures for susceptibility testing of aerobic and facultatively anaerobic bacteria isolated from clinical specimens. Middlebrook 7H10 w/OADC Enrichment Agar 20 ml/plate; 20/sleeve 21-40S67 Used in qualitative procedures for the isolation and cultivation of mycobacteria. Modified Clostridial Broth 100 ml/bottle 26-577 A semi-solid medium for the enumeration and cultivation of clostridia and other anaerobes occurring in food and pathological specimens. Nutrient Agar For the cultivation of bacteria and for the enumeration of organisms in water, sewage, feces and other materials. Plates 20 ml/plate; 10/sleeve 21-40S76 Plates (Oxoid No. 2) 30 ml/plate; 10/sleeve 21-100 Plates (Heavy Fill) 45 ml/plate; 10/sleeve 21-40S129 Slants 10 ml/tube; 10 or 100/box 22-132 Nutrient Broth (BD) For the cultivation of nonfastidious microorganisms. Tubes 9 ml/tube; 10 or 100/box 22-121 Nutrient Broth, Oxoid No. 2 For culture of S. typhimurium and E. coli strains. Prepared as described by Maron and Ames (Mutat. Res., 113: 173, 1983) 100 ml/bottle 26-505.1 300 ml/bottle 26-505.3 Nutrient Broth, Oxoid No. 2. with VBE salts For culture growth of S. typhimurium Ames strains and E. coli WP2. 300 ml/bottle 500 ml/bottle 26-555.3 26-555.5 Phytone Yeast Extract Agar Used for the selective isolation of dermatophytes, particularly Trichophyton verrucosum, and other pathogenic fungi from routine clinical specimens. 200 ml/bottle 51-40S048 500 ml/bottle 51-40S049 Plate Count/Standard Methods Agar Also known as Tryptone Glucose Yeast Agar. Used for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance. Plates 25 ml/plate; 20/sleeve 21-40S46 Bottles 400 ml/bottle 26-521 www.MOLTOX.com 800.536.7232 11 PRODUCT SIZE Potassium Tellurite Agar For the cultivation and differentiation of Enterococcus faecalis. 20 ml/plate; 20/sleeve Tubes; 10/box PART # 21-40S91 22-113 Potato Dextrose Agar Used for the cultivation and enumeration of yeasts and molds. Conforms with specification of The United States Pharmacopeia (USP). Recommended by the American Public Health Association for plate counts of yeasts and molds in the examination of foods and dairy products. Recommended in the USP for use in the performance of Microbial Limit Tests. 20 ml/plate; 10/sleeve 51-40S036 20 ml/plate; 20/sleeve 21-40S52 Contact plates; 10/sleeve 51-40S131 Potato Dextrose Agar, low pH (3.5) 20 ml/plate; 20/sleeve 21-40S32 Used for the cultivation and enumeration of yeasts and molds. The low pH inhibits bacterial growth. Conforms with specification of The United States Pharmacopeia (USP). Recommended by the American Public Health Association for plate counts of yeasts and molds in the examination of foods and dairy products. Recommended in the USP for use in the performance of Microbial Limit Tests. Potato Dextrose Agar w/ Chlortetracycline & Chloramphenicol 20 ml/plate; 10/sleeve 21-40S74 Used for the cultivation and enumeration of yeasts and molds. The low pH, chlortetracycline and chloramphenicol inhibit microbial growth. Potato Starch Agar 20 ml/plate; 20/sleeve 21-40S192 Used in qualitative procedures for the differentiation of Nocardia species. Part of a set of Nocardia Differentiation Media (Casein Agar, Tyrosine Agar, Xanthine Agar, & Starch Agar). Pseudomonas Agar F 10 plates/sleeve 21-40S77 Used for the differentiation of fluorescein and pyocyanin producing Pseudomonas. Pseudomonas Agar P 10 plates/sleeve 21-40S75 Used for the differentiation of fluorescein and pyocyanin producing Pseudomonas. R2A Agar Used for enumerating heterotrophic organisms in treated potable water. 20 ml/plate; 10/sleeve 20 ml/plate; 20/sleeve 51-40S081.140 21-40S13 Rappaport-Vassiliadis R10 Broth 10 ml/tube; 10/box 22-138.010 Used in the cultivation and recovery of Salmonella from various samples. Reinforced Clostridial Agar Used for cultivating and enumerating clostridia, other anaerobes, and other species of bacteria. A non-selective enrichment medium that grows various anaerobic and facultative bacteria when incubated anaerobically. 20 ml/plate;10/sleeve 21-40S145 100 ml/bottle 26-612 Rose Bengal Agar Used to isolate and enumerate yeasts and molds. Recommended for the enumeration of yeasts and molds. Contains Chloramphenicol (also known as Antimicrobic Supplement C). Contact plates; 10/sleeve 21-40S68 20 ml/plate; 10/sleeve 21-40S48 20 ml/plate; 20/sleeve 21-40S63 12 MOLTOX® Bacteriological Media PRODUCT SIZE PART # Sabouraud Dextrose Agar General-purpose medium for the cultivation of pathogenic and nonpathogenic fungi. Recommended in the USP for use in performing total combined mold and yeast counts (Microbial Limit Tests). Plates 20 ml/plate; 20/sleeve 21-40S53 Slants 10 ml/tube; 10 or 100/box 22-106 w/ Chloramphenicol - Plates 27 ml/plate; 10/sleeve 21-40S277.140 w/ Chloramphenicol - Contact Plates 10/sleeve 21-40S48 w/ Chloramphenicol - Slants 10 ml/tube; 10 or 100/box 22-112 w/ Lecithin & Tween 80 - Plates 20 ml/plate; 10/sleeve 21-40S101 Sabouraud Dextrose Broth w/ Chloramphenicol 100 ml/bottle 26-708 General-purpose medium for the cultivation of pathogenic and nonpathogenic fungi. Salmonella Shigella Agar 20 ml/plate; 10/sleeve 51-40S011 Moderately selective media used in the isolation of pathogenic enteric Salmonella. Skim Milk 15% 50 ml/bottle 21-40S50 Used for preparing microbiological cultures and differentiation of microorganisms based on coagulation and proteolysis of casein. 5 plates/sleeve 21-200 ST QUAD PC™ For phenotypic confirmation of S. typhimurium Ames strains. Tests for histidine auxotrophy, pKM101, pAQ1 and rfa. Tetrathionate Brilliant Green 10 ml/tube; 10/box 22-104 A selective enrichment medium used in the isolation of Salmonella from food, feces, and other materials of sanitary importance. Tomato Juice Agar 20 ml/plate; 20/sleeve Used for cultivating and enumerating Lactobacillus species. 21-40S90.141 Tomato Juice Broth 100 ml/bottle Used for cultivating yeasts and other aciduric microorganisms. 26-584 Top Agar Used to overlay materials onto plated agar. Requires supplementation with histidine/biotin if used with Minimal Glucose Agar and S. typhimurium Ames strains. 100 ml/bottle 26-501.1 250 ml/bottle 26-501.25 300 ml/bottle 26-501.3 600 ml/bottle 26-501.6 Triple Sugar Iron Agar Used to differentiate gram-negative enteric bacilli based on the fermentation of carbohydrates and hydrogen sulfide production. Slants 10 ml/tube; 10/box 22-109.010H Tryptic Soy Agar Specified as Soybean-Casein Digest Agar Medium in the USP. Used for the isolation and cultivation of nonfastidious and fastidious microorganisms. Plates Contact plates; 10/sleeve 51-40S078 20 ml/plate; 20/sleeve 21-40S54 30 ml/plate; 10/sleeve 21-40S259.140 Tubes 10 ml/tube; 10 or 100/box 22-107 45 ml/tube; 10/box 22-144.031 www.MOLTOX.com 800.536.7232 13 PRODUCT SIZE PART # Tryptic Soy Agar w/Lecithin and Tween 80 For the detection and enumeration of microorganisms present on surfaces of sanitary importance. Contact plates; 10/sleeve 21-40S108 20 ml/plate; 10/sleeve 21-40S100 Tryptic Soy Agar w/ 5% Sheep’s Blood Used for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. 20 ml/plate; 10/sleeve 51-40S003 20 ml/plate; 20/sleeve 21-40S61 10 ml/tube; 10 or 100/box 22-110 Tryptic Soy Broth General Purpose medium for the cultivation of fastidious and nonfastidious microorganisms. Tubes 3 ml/tube; 10 or 100/box 22-114 10 ml/tube; 10 or 100/box 22-127 20 ml/tube; 10/box or 100/box 22-125 25 ml/tube; 10 or 100/box 22-126 30 ml/tube; 10 or 100/box 22-135 Bottles 100 ml/bottle 26-607 1000 ml/bottle 26-531 w/ 0.5% Lecithin & 4% Tween 20 90 ml/bottle 26-625 Tryptophan Top Agar For use with Minimal Glucose Agar and E. coli WP2 strains. 100 ml/bottle 300 ml/bottle 26-502.1 26-502.3 Tryptose Phosphate Broth Used for cultivating fastidious microorganisms. 100 ml/bottle 500 ml/bottle 26-615 26-618 Tyrosine Agar 20 ml/plate; 20/sleeve 21-40S191 Used in qualitative procedures for the differentiation of Nocardia species. Part of a set of Nocardia Differentiation Media (Casein Agar, Tyrosine Agar, Xanthine Agar, & Starch Agar). V8 Agar Used for the sporulation of various fungi. 20 ml/plate; 20/sleeve 10 ml/tube; 10/box 21-40S49 22-103 Violet Red Bile Agar Used for enumerating coliform organisms in dairy products. Contact Plates; 10/sleeve 20 ml/plate; 10/sleeve 21-40S260 51-40S090 Violet Red Bile Glucose Agar 20 ml/plate; 20/sleeve 21-40S56 Used in the detection and enumeration of Enterobacteriaceae in dairy and other foods. XLD Agar 20 ml/plate; 10/sleeve 51-40S016 A moderately selective medium used in the isolation and differentiation of enteric pathogens, mainly Shigella species. Yeast Malt Agar Used to cultivate yeasts, molds, and other aciduric microorganisms. Contact Plates; 10/sleeve 20 ml/plate; 20/sleeve 14 MOLTOX® Bacteriological Media 21-40S114 21-40S88 Multi-Section Plates PRODUCT SIZE PART # Bismuth Sulfite/Brilliant Green/XLD Tri Plate 10 plates/sleeve 21-40S73 Used for the differentiation and isolation of Enterobacteriaceae. Bismuth Sulfite – Used for isolating Salmonella spp., particularly S. typhi. Brilliant Green – Used for isolating Salmonella. XLD – Used for isolating and differentiation of enteric pathogens, especially Shigella species Pseudomonas P & F Agar Bi Plates 10 plates/sleeve 21-40S79 Used for the differentiation of fluorescin and pyocyanin producing Pseudomonas. Pseudomonas P – Also known as Tech Agar; used for enhancement of pyocyanin production by Pseudomonas. Pseudomonas F – Also known as Flo Agar; used for the enhancement of fluorescin production. Pseudomonas P, Pseudomonas F, Cetrimide Agar Tri Plates 10 plates/sleeve 21-40S94 Used for the selective isolation, differentiation, and identification of Pseudomonas sp. Pseudomonas P – Also known as Tech Agar; used for enhancement of pyocyanin production by Pseudomonas. Pseudomonas F – Also known as Flo Agar; used for the enhancement of fluorescin production. Cetrimide – Also known as Pseudosel™ Agar. Used for the selective isolation and identification of Pseudomonas aeruginosa. Cetrimide/Mannitol Salt/MacConkey Tri Plates 10/sleeve 21-40S78 Cetrimide – Used in the selective isolation and identification of Pseudomonas aeruginosa. Mannitol Salt – Used for the selective isolation and enumeration of staphylococci. MacConkey – Used for isolating and differentiating lactose-fermenting from lactosenonfermenting gram-negative enteric bacilli. www.MOLTOX.com 800.536.7232 15 Reagents/Buffers PRODUCT SIZE PART # Butterfields Phosphate Buffer, pH 7.2 9 ml/tube; 10/box and 100/box 22-120 1000 ml/bottle 26-622 Glucose, 3% 500 ml/bottle 26-643.047 Glucose, 20% 500 ml/bottle 26-538 700 ml/bottle 26-542 Glycine Buffer, 0.2 M, pH 2.8 500 ml/bottle 26-533 Histidine/Biotin, 0.5 mM 100 ml/bottle 26-700 L-Tryptophan, 0.5 mM 100 ml/bottle 26-701.1 Magnesium Chloride, 0.25 M 50 ml/bottle 26-646 10 ml/bottle 26-512.010 20 ml/bottle 26-512.020 MgCl2, 0.4 M /KCl, 1.65 M Magnesium Sulfate, 10 mM 1000 ml/bottle 26-601 NaCl, 1.0 M, pH 8.3 1000 ml/bottle 26-574 NaCl, 2.0 M 1000 ml/bottle 26-568 NaHCO3, 0.2 M /0.5 M NaCl Buffer, pH 8.3 1000 ml/bottle 26-535 Potassium Chloride, 0.8 M 5 ml/tube; 10/box 22-111 10 ml/tube; 10/box and 100/box 22-128 Potassium Chloride, 1 M 50 ml/bottle 26-645 Saline Solution, Sterile, 0.9% 20 ml/tube; 100/box 22-143 1000 ml/bottle 51-40S022 Sodium Acetate Buffer, 0.1 M, pH 4.0 1000 ml/bottle 26-573 Sodium Butyrate, 500 mM 1000 ml/bottle 26-578 Sodium Phosphate Buffer, 0.1 M, pH 7.4 100 ml/bottle 26-510.039 500 ml/bottle 26-510.047 Sodium Phosphate Buffer, 1 M, pH 7.4 50 ml/bottle 26-588 Sodium Phosphate Buffer, 2.0 M, pH 7.4 500 ml/bottle 26-516.5 Sterile Distilled Water, pH 5.5-7.0 15 ml/tube; 10/box 22-140 Sucrose, 20% 1000 ml/bottle 26-585 Tris-HCl, 20 mM, pH 8.6 1000 ml/bottle 26-566 Tris-HCl, 40 mM, pH 8.6 1000 ml/bottle 26-569 Tris-HCl, 1.0 M, pH 9.0 1000 ml/bottle 26-534 16 MOLTOX® Bacteriological Media www.MOLTOX.com 1.800.536.7232
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