[CV SARA FAHLÉN ] 1
PhD in Molecular Genetics
-with a special interest in proteins and polysaccharides
Personal data
Sara Fahlén(former Johansson)
Born: 1976 in Luleå
Married, three children
070-3359476, [email protected]
Swedish Medical Nanoscience Center
Department of Neuroscience
Karolinska Institute
171 77 Stockholm
Laborative expertise
Biochemical/protein methods; protein preparation from mammalian, plant and bacterial cells,
membrane protein isolation, antibody production, antibody purification, chromatography
methods, co-immunoprecipitation, SDS-PAGE, western blot, GTPase activation, GST-protein
purification, GST-pulldown, in vitro translation, in vitro interaction methods, S35-labelling,
pulse-chase, cycloheximide chase, ubiquitylation assays
Chemical methods; freeze drying, fourier transform infrared spectroscopy (FTIR), NMR and
ion chromatography of polysaccharides prepared from biofilm
Microbiology methods; biofilm growth, extraction of extracellular matrix from biofilm,
growth curves, single streak of colonies, invasion of eukaryotic cells
Molecular methods; transformation of bacteria, plasmid preparation, PCR, cloning,
mutagenesis, RNA extraction, cDNA assay, RT-qPCR, northern blot, reporter-constructmethods
Mammalian cell methods; culturing of mammalian suspension and adherent cells,
construction of stable cell lines, cell culturing in hypoxic chamber, viral transduction,
transfection, siRNA, cell cycle synchronisation assays, inducible protein expression assays,
cell proliferation assays, immunohistochemistry, cell stress assays
Plant cell methods; induction and culturing of tobacco pollen tubes, culturing of Nicotiana
tabaccum plants
Project management skills
Organizational experience:
Organization and management of routines for daily lab work 2013-2014
Initiation and organization of a new cell bank 2013-2014
Organization of two annual retreats (2012, 2011) for 35 people, tasks including inviting
speakers for seminars, organizing group tasks, social activities, transports and housing
Organizing monthly seminar series 2011-2013
Organizing monthly/second monthly project meetings 2011-2013
Courses:
Pedagogics (9hp) 2003, Swedish University of Agricultural Sciences, SLU
Leadership (1.5hp) 2004, Swedish University of Agricultural Sciences, SLU
2 [CV SARA FAHLÉN ] Communication skills
Courses:
Popular science writing (one week full time) 2012, Karolinska Institute
How to present research (1,5hp) 2002, Swedish University of Agricultural Sciences, SLU
Informera på www (4,5hp) 1998, Swedish University of Agricultural Sciences, SLU
Scientific writing: doctoral thesis http://pub.epsilon.slu.se/1897/, main author on one
manuscript, co-author on three published papers (see “publications” section) and three
manuscripts.
Other activities: writing popular science summaries for grant applications, writing
applications to Arbetsmiljöverket, writing SOPs and risk assessments, writing
recommendation letters, proofreading of applications, manuscripts and reports, national and
international poster presentations, national and international oral presentations, responsible for
webpage layout and administration for one course web page and for two consortium web
pages (see “employment” section)
Employment
2013 OctResearch engineer, Swedish Medical Nanoscience Center, Karolinska Institute
present
www.medicalnanoscience.se
Research project: extraction and characterization of polysaccharides from
bacterial biofilm (including design of experiments, bench work, analysis of
results)
Other tasks: responsibility for implement and transition to electronic laboratory
notebooks for the research group, popular science writing and proof reading of
applications and reports, laboratory equipment responsibility (maintainance
and new purchases including procurement), set up and organization of cell
bank, responsibility for cell culturing facility, chemical-, biological- and
isotope- responsibility (risk assessments, inventory, permissions from
Arbetsmiljöverket), administration of students, data storage responsibility,
database management
2013 Feb2013 Sept
Lab manager, Swedish Medical Nanoscience Center, Karolinska Institute
Tasks: organization and management of routines for daily lab work (e.g.
making SOPs and risk assessments), laboratory equipment responsibility
(maintainance and new purchases including procurement), chemical-,
biological- and isotope- responsibility (risk assessments, inventory,
permissions from Arbetsmiljöverket), work environment responsibility,
introduction of new staff members, set up and organization of cell bank,
popular science writing and proof reading of applications and reports, webpage
layout and development, data storage responsibility, database management
2011 May2013 Jan
Project coordinator ACT! (Advanced Cancer Therapies) network, Karolinska
Institute, http://ki.se/en/research/the-centre-for-advanced-cancer-therapies-act
Tasks: Management of a network comprising twelve professors and their
research groups respectively, e.g. organizing conferences and seminars,
maintaining cooperations, webpage administration, English-Swedish
translation of documents, proof reading of documents, other administrative
tasks
[CV SARA FAHLÉN ] 3
2011 May2013 Jan
Post doc, Karolinska Institute
Project title: Regulation of c-Myc Function by a Tumour Supressor Protein
Project contents: Identification and characterization of the biological function
of c-Myc interaction with a tumour suppressor protein.
Outcome: Two manuscripts, establishment and development of a cell based
stress assay, construction of stable siRNA knock down cell lines, development
of coIP interaction assay with a wide panel of mutant proteins
2009 March- Post doc, Swedish University of Agricultural Sciences, SLU
2011 May
Project title: Mechanism and Regulation of Polar Cell Growth
Project contents: Biochemical identification and characterization of Rho
signaling complexes in tobacco pollen tubes.
Outcome: Two manuscripts, establishment and development of the following
tools (not previously existing in the lab); membrane protein isolation method,
membrane protein identification and characterization method, protein
production and purification for antibody production, SDS-PAGE and western
blot
2002 April2009 Feb
Education
2002 Apr2008 Dec
Teaching assistant, Swedish University of Agricultural Sciences, SLU
Tasks: teaching, including laboratory practicals (development and organization
of 1-3 weeks long practicals for 10-20 students), tutorials and supervision of
undergraduate students
Laboratory practicals: Protein expression in yeast (main responsible 2006),
Apoptosis (main responsible 2006), Gene expression and regulation in
mammalian cells (main responsible 2005, 2004, co-responsible 2003, 2002),
Cell culturing (main responsible 2005, 2004, 2003)
Supervision of individual undergraduate student projects:
2009 Jan-Feb
Amareswar Vangala, master student
2006 June-Sept
Linda Sooman, project student
2006 Sept, 2005 June-July Anna Svensson, project student
PhD in Molecular Genetics at Swedish University of Agricultural Sciences,
SLU
Thesis title: Regulation of Myc Oncoprotein Function by E3 Ubiquitin Ligases
Thesis content: Identification of protein binding partners, post-translational
modifications, cofactors and degradation mechanisms for the transcription
factor/oncoprotein Myc.
1997 Aug2002 March
MSc in Biology at the Biotechnology programme at Swedish University of
Agricultural Sciences, SLU
Master thesis: Studies of in vivo and in vitro Interactions between the
Oncoprotein c-Myc and the E3 Ligase Degradation Complex Skp2, 30hp
2000 JanJune
Erasmus scholarship within the Biotechnology programme, University of Gent,
Gent, Belgium
1999 SeptDec
Exchange studies within the Biotechnology programme, University of New
South Wales, Sydney, Australia
4 [CV SARA FAHLÉN ] 1996 Aug1997 Jan
Mathematics A (30 hp) at Luleå University of Technology
1995 SeptDec
Cambridge First Certificate in English at Queensland College of English
Brisbane, Australia
Popular science publications
Fahlén S, Projektledaren som blev toppchef. Naturvetare. 2013 May; 3: p46-48
Scientific publications
Stephan O; Cottier S; Fahlén S; Montes-Rodriguez A; Sun J; Klahre U; Kost B, Nt-Risap is a
TGN associated Nt-Rac5 effector regulating membrane traffic during polar cell growth in
tobacco, submitted to Plant Cell, March 2014.
Stephan O; Cottier S; Fahlén S; Klahre U; Kost B, The N. tabacum RIPA1 gene encodes for a
novel pollen tube protein involved in Rac5 GTPase dependent control of polarized cell growth
Conference: 22nd IUBMB Congress/37th FEBS Congress Location: Seville, SPAIN Date:
SEP 04-09, 2012. FEBS J. 2012 Sep; 279(Issue Supplement S1): 70-70. DOI: 10.1111/j.17424658.2010.08705.x
Arabi A; Wu S; Ridderstråle K; Bierhoff H; Shiue C; Fatyol K; Fahlén S; Hydbring P;
Söderberg O; Grummt I; Larsson LG; Wright AP. c-Myc associates with ribosomal DNA and
activates RNA polymerase I transcription. Nat Cell Biol. 2005 Mar; 7(3): 303-10
von der Lehr N; Johansson S; Larsson LG. Implication of the ubiquitin/proteasome system in
Myc-regulated transcription. Cell Cycle. 2003 Sep-Oct; 2(5): 403-7
von der Lehr N; Johansson S; Wu S; Bahram F; Castell A; Cetinkaya C; Hydbring P;
Weidung I; Nakayama K; Nakayama KI; Söderberg O; Kerppola TK; Larsson LG. The F-box
protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for cMyc-regulated transcription. Mol Cell. 2003 May; 11(5): 1189-200
International employment
2000 July
Database manager, Crop Design, Belgium
Tasks: classification of scientific papers and making databases
1997 JanApril
Voluntary work, Kibbutz Sde Nehemia, Israel
1996 FebJune
Bartender, Scottish and Newcastle, England
Language skills
Swedish – mother thong
English – fluent in speech and writing
Dutch – beginner
Computer skills
Office – experienced
Adobe Photoshop – experienced
[CV SARA FAHLÉN ] 5
Adobe Illustrator - good
Joomla – experienced
X-mind – experienced
GraphPad - experienced
Parental leave
Aug 2005-March 2006, Aug 2007-March 2008 and March 2010-Dec 2010