™
www.peptest.co.uk
™
www.peptest.co.uk
RD Biomed Limited
Daisy Building (2nd Floor),
Castle Hill Hospital, Castle Road,
Hull, HU16 5JQ
Tel: +44 (0)1482 461877
Email: [email protected]
www.rdbiomed.com
Pepsin can be detected rapidly and
non-invasively using Peptest™
Peptest™ is an innovative test for quickly and easily identifying patients who
reflux gastric juice.
Gastric juice comprises ingested food
and drink, acid and the digestive
enzyme pepsin. All these components
can be refluxed from the stomach into
the
oesophagus,
larynx
and
respiratory system. Reflux of gastric
juice is usually identified by detecting
refluxed acid (H+ions) using 24 hr
pHmetry or detecting refluxed fluid
using 24 hr impedance monitoring.
Both these techniques are highly
invasive and in the case of pHmetry
require the gastric fluid to be <pH 4.
Peptest™ permits the non-invasive, pH
independent identification of patients
suffering from gastric reflux. It detects
pepsin in clinical samples obtained
from the upper digestive tract and the
respiratory system. The presence of
pepsin in these samples is a reliable
marker for the reflux of gastric juice.
Pepsin is the enzyme responsible for
the crude digestion of protein in the
diet. It is also the main aggressive
agent responsible for damaging
oesophageal and laryngeal tissue
during reflux. Detection of pepsin in
the refluxate is an appropriate
objective clinical measurement tool
because it specifically detects the
damaging component of gastric
refluxate.
Furthermore, detection of pepsin and
the identification of a reflux event is
independent of the pH of gastric fluid.
Peptest™ can detect
pepsin in a wide
range of clinical
samples including:
›› Saliva
›› Sputum
›› Tracheal aspirate
›› Oesophageal aspirate
›› Gastric juice (requires dilution)
›› Exhaled breath condensate
›› Bronchoalveolar lavage fluid
Peptest™ can be used to identify
the presence of pepsin, which
can contribute to the pathology
of a wide spectrum of conditions:
›› GORD
›› EOR
›› LPR
›› Chronic cough
›› Cystic fibrosis
›› Lung allograft rejection
›› Otitis media with effusion
›› Asthma
›› Sinusitis
Peptest™ detection limit 16ng/ml pepsin,
sensitivity 88% and specificity 87%.
™
Application
80µl (clinical sample + buffer)
added to well of LFD. Pepsin
detected by latex bead
labelled pepsin detection
monoclonal antibody.
Lateral Flow Device
(LFD)
Latex
Beads
Detection
monoclonal
antibody directly
labelled with blue
latex beads
Pepsin
Pepsin
‘T’ line visible after
5 minutes if pepsin
present in clinical sample.
Capture monoclonal
antibody immobilized
on membrane of
LFD (’T’ line)
Visualisation
Capture pepsin monoclonal
antibody present at ‘T’ line and
control secondary antibody
present at ‘C’ line.
MHRA Registered
Case Studies
›› Acid Suppression
›› Pepsin has been detected in saliva ››
samples of patients experiencing
›› persistent symptoms whilst on PPI
›› therapy.
›› There is no difference in prevalence ››
of pepsin between GORD patients
››on or off PPI therapy.
››Provides some evidence for a
›potential role of ‘weak-acid’ reflux
››in causing symptoms.
›› Paediatrics
›› Peptest™ can be used in children.
›› Pepsin has been detected in
›› regurgitation samples of children
›› experiencing paediatric reflux.
›› Cystic Fibrosis
›› A saline lung lavage from a cystic
›› fibrosis patient was positive for
›› pepsin using Peptest™.
Peptest™ provides important information to aid the diagnosis of certain diseases. Test results should be used with other clinical
and diagnostic findings to make a diagnosis.