AQUACULTURE USING COLD OTEC WATER byThomas H. Daniel,LaboratoryDirector The Natural Energy Laboratory ofHawaii P.O. Box 1749 Kailua-Kona, Hawaii 96745 ABSTRACT The unique 2000 f t deep seawater pipeline installed a t theNatural Energy Laboratory ofHawaii (NELH) suppliestheonlycoldseawateravailableinlarge volumes anywhere i n thetropics. Not onlydoes the low temperature of thecold OTEC processpermitthe seawaterrequiredforthe tropicalculture of species normally found onlyin colder areas, i t a1 so allows inexpensive yet precisetemperaturecontrolthroughout even anopen high culture system. The deepwateralsocontains concentrations of the dissolved inorganic nutrients essential to p l a n t growth, whiletheneartotal absence of pathogens,plants and particulates makes the water particularly well suited for the culture of sensi tive organisms and also for the development of pure c u l t u r e s t r a i n s . THE NATURAL ENERGY LABORATORY OF HAWAII - A BRIEFHISTORY The Hawaii State Legislature founded theNatural Energy Laboratory ofHawaii ( N E L H ) in 1974. The enabling legislation set aside 322 a c r e s a t Keahole P o i n t on the Big Island ofHawaii (Fig. 1) forresearch i n t o renewableenergy resources and also established a Managing Board of S t a t e , County and Universityofficials t o oversee i t s development. b Lana: of<-% n HAWAII 1284 From 1974 t o 1979, theFederal DepartmentofEnergy (DOE) sponsored some experiments onOceanThermal EnergyConversion (OTEC) which wereperformed a t NELH by researchers from various universities. D u r i n g the same years, acomprehensive permitting program established all the permits necessary for performing researchonshore and offshore a t NELH. Mfni-CTEC, t h e w o r l d ' s f i r s t n e t power-producing OTEC plant, operated successfully under state and privateindustrysponsorship i n NELH's offshore researchcorridor i n l a t e 1979.In the same year, construction began on the Seacoast Test Facility (STF), a j o i n t state/DOE project aimed a t providing laboratory facilities for studying OTEC processes. Only asmall portion of thefederal money earmarked so the f o r STF construction ever materialized, state increased its contribution and applied "creativeengineering" t o construct an operating laboratoryforaboutone-halftheoriginal planned $16 millioncost.Thislaboratoryhas met o r exceeded a l l of the goals originally set for the full scale facil ity.1 The S t a t e o f Hawaii operates NELH t o encourage research , development and comnercial ization of a l t e r n a t e energy and relatedtechnologies.Since Hawaii by i t s e l f does n o t have sufficient financial or human resources t o perform the needed research, t h e s t a t e ' s goal i s t o provide a f a c i l i t y where others from b o t h thepublic and private sectors can perform theexperimentation. I t i s planned t h a t thefacilitywill soon become self-supporting from user fees. The DOE remains the major user of NELR f a c i l i t i e s , now providingabout 60%of theoperationalfunding. The s t a t e has made e f f o r t s t o broaden the NELH supportbase by encouragingthe i n i t i a t i o n of a range of new projects i n aquaculture and related f i e l d s sponsored by bothgovernment and industry. The s t a t e l e g i s l a t u r e hasrecognizedthepotential of these new p r o j e c t s , f i r s t by establishing "commercialization" as a legitimate goal of NELH and, most recently, by i n i t i a t i n g t h e development of the HawaiiOcean Science and Technology (HOST) P a r k adjacent t o NELH. Laboratoryusers can now plan on b o t h pilot scale comercial operations on land leased from NELH and eventualexpansion t o l a r g e r f a c i l i t i e s i n the HOST Park. THENELHCOLDMATER PIPE The i n i t i a l STF d e s i g n c a l l e d f o r i n s t a l l a t i o n o f t h r e e3 2 in. p o l y e t h y l e n ep i p e s : a m i l e - l o n gd e e p p i p e f o r c o l d w a t e r , a n e a r s h o r e p i p e f o r warm s u r f a c ew a t e ra n da 7 d i s c h a r g ep i p ef o ro f f s h o r e d i s c h a r g ea f t e re x p e r i m e n t s .T h e s ep i p e sw e r e t o r u n t h e 380 f t f r o m t h e s h o r e l i n e t o a pump s t a t i o na tt h el a b o r a t o r yt h r o u g h at r e n c he x c a v a t e d t o 8 f t b e l o ws e a l e v e l .T h et r e n c h was n e c e s s a r y s i n c e a s t a t i c head i s r e q u i r e d o v e r t h e i n t a k e p i p e sa n dt h e pumps t o p r e v e n t pump c a v i t a t i o n c a u s e db yt h es u c t i o nh e a dd e v e l o p e di ns u c k i n g t h r o u g h a m i l e - 1 ong p i p e . 2 When f u n d i n g was reduced,engineersdeveloped a c h e a p e r" i n t e r i m "s y s t e m ,w h e r et h ep i p e sr u nu p o v e rt h es e a - c l i f fa tt h es h o r e l i n e .T h i sd e s i g n n e c e s s i t a t e st h ei n s t a l l a t i o no ft h ed e e pw a t e r pumps o f f s h o r e t o p r o v i d e t h e r e q u i r e d s t a t i c h e a d . The s u r f a c e w a t e r p i p e f o r t h i s i n t e r i m s y s t e m was i n s t a l l e d as p a r t o f t h e Phase I c o n s t r u c t i o n o f 500 GPM pumps t h e STF i n 1980. I t sf o u ro n s h o r e provide a totalcapacityof 2000 GPM, and w a t e rh a s been pumped t h r o u g ht h es y s t e mc o n t i n u o u s l ys i n c e J u l y o f 1981. Nhen i t became c l e a r t h a t DOE f u n d i n g f o r t h e r e m a i n d e r o f t h e STF w o u l d n o t m a t e r i a l i z e , t h e s t a t et o o kt h ei n i t i a t i v ea n di n s t a l l e dt h ei n t e r i m c o l dw a t e rs u p p l ys y s t e m ,u s i n gC a p i t a lI m p r o v e m e n tP r o . i e c tf u n d se a r m a r k e df o ra a u a c u lt u r e .P i D e __ .. \ * b o t t o ma n c h o r i n s t a l l a t i o n was c o m p l e t e d i n December1981,and thesystembeganpumpingcoldseawater i n February 1982.Deepseawaterhasflowedonshorecontinuousl y s i n c e i n i t i a l pumpingproblemswere rectifiedin A u g u s t 1982. F i g u r e 2 shows t h ed e s i g no ft h e 12 i n .d i a m e t e r J. van NELH i n t e r i m c o l d w a t e r p i p e l i n e p r e p a r e d b y R y t i n o f Makai Ocean E n g i n e e r i n g i n Waimanalo, H a w a i i .T h eo f f s h o r ep o r t i o n , made f r o ms l i g h t l y b u o y a n th i g hd e n s i t yp o l y e t h y l e n e , i s h e l d on t h e b o t t o mw i t hc o n c r e t ea n c h o r s down t o t h e 500 f t d e p t h ,b e l o ww h i c ht h eb o t t o md r o p so f fm o r es t e e p l y . From t h a t 500 f t t r a n s i t i o np o i n t ,t h ep i p e f l o a t s i n a b u o y a n tc a t e n a r y down t o t h e 2000 f t d e p t hw h e r el a r g eb a t t l e s h i pa n c h o r sh o l dt h ei n The pump s t a t a k ea b o u t 100 f t o f ft h eb o t t o m . tion,locatedinabout 30 f t o fw a t e ra p p r o x i m a t e l y 100 f t o f f s h o r e , c o n t a i n s t h r e e h o r i z o n t a l l y - m o u n t e di n - l i n es u b m e r s i b l e pumps a r r a n g e d i n p a r a l l e l . Power f o r t h e pumps comes througharmoredcables f r o ms h o r e . E x p e r i m e n t a lr e q u i r e m e n t sd i c t a t e dt h a tt h ec o l d w a t e r pumps n o t d i s s o l v e i r o n i o n s i n t o t h e w a t e r . S i n c ef i b e r g l a s so rp l a s t i cl i n e ds u b m e r s i b l e pumps w e r eu n a v a i l a b l e , 316 s t a i n l e s s s t e e l was s p e c i f i e d f o ra l w l e t t e dc o m p o n e n t s . Pump performancehas n o ty e tm e ts p e c i f i c a t i o n s ,d u et om a n u f a c t u r e r i n e x p e r i e n c ew i t h SS c a s t i n g s . Non-316 SS compone n t sh a v ea l s ob e e nr e p e a t e d l yd i s c o v e r e d ,i ns p i t e o fc e r t i f i c a t i o n st ot h ec o n t r a r y .W i t ht h et h r e e pumps now i n s t a l l e d ,t h es y s t e mp r o d u c e su pt o 1100 .. 200 ELEVATION dnn 7"" '\ Concrete anchors 600 800 1001 L 1200 a aJ 1400 n 2000 F i g u r e2 .T h e 'm a x .c u r r e n t location P i p eM a t e r i a l s : No V e r t i c aEl x a g g e r a t i o nO f f s h o r e - H i g Dh e n s i t Py o l y e t h y l e n e Onshore - PVC D i a m e t e r - 12 i n . NELH C o l d w a t e rP i p e l i n e 1285 COLD WATER PROPERTIES GPM. Two new pumps planned f o ri n s t a l l a t i o n i n September1985 willtogetherprovidethe 1300 GPM designcapacity of the system. One of thepresent pumps will remain installed as a backup. A regular sampling program has been maintainedfor the three years that cold water has beenpumped, as part of the DOE-sponsored OTEC experiments. Weekly surface and deepwater measurements of s a l i n i t y , a l k a l i n i t y , pH, dissolved oxygen, dissolved nut r i e n t s (NO3,PO4,& SiO3),totaldissolvednitrogen and phosphorus, totalorganiccarbon, and total suspendedsolidssupplement daily measurements of temperature and hypochlorite concentration. for instalThe NELH s t a f f hasdevelopedtechniques ling and removing thecoldwater pumps in most wave and currentconditions from asmallboat.Maintenance becomes impossible, however, d u r i n g extreme wave conditions which occur 20 t o 30 daysperyear. In s p i t e of t h e f a c t t h a t system f a i l u r e s tend t o coincide w i t h extreme conditions, hard workand a generous amount o f good luck have permitted nearly continuous operation for more than three years. TableI summarizes t h e r e s u l t s of these measurefrom traditional oceanoments. As waswe11-known graphic data, the deep water i s s i g n i f i c a n t l y enrichedoverthetropicalsurfacewater i n the dissolvedinorganicnutrients which areessential t o plant growth. The intaketemperature of thecold waterstayswithintherange of 5.5OC - 6.5"C The temper(42.4"F - 43.7"F) throughouttheyear. ature delivered on shore is a function of the flow a t 500 GPM thetemperature is rateinthepipe: about 10°C (50°F) , a t 1000 GPM i t i s approximately 7.5OC (45.5"F). The polyethylenepipessurvivedthelarge waves generated by Hurricane Iwa i n November 1982, b u t damage t o the armored electricalcablesresulted in severalshort-duration pump outagesoverthe ensuing two months. No flow interruptions exceeding two hoursoccurred a f t e r t h a t u n t i l l a r g e waves againbrokethe armored power cablesinJanuary 1985. A 37 houroutage a t t h a t timepermitted i n s t a l l a t i o n of new specially designedcaged-armor cables which shouldensurecontinuousoperationas l o n g as the pumps operate and the pipeline remains intact. Three properties of thecoldwater ularlyusefulforaquaculture: 1) Coldness - Not onlydoes thecoldwaterpermit the culture of plants and animals which couldnot otherwisetoleratetropicaltemperatures, i t also provides a simple,accurate and cost-effective throughout meansof year-roundtemperaturecontrol a culturing system. Control of therate of water The NELH coldseawater system i s believedto be the only one i n the world which continuouslybrings pureocean water from 2000 f t deep i n the sea to shoreforexperimentation and comercialventures. Planning i s underway foradditionalpipelinesto be i n s t a l l e d a t NELH by b o t h industry and government. PARAMETER Table 1. WATER QUALITY DATA - NELH WARM AND COLD SEAWATER SYSTEMS (Weekly Sampl es 1982-84) Ut4 IT WARM SEAWATER SYSTEM COLD SEAWATER SYSTEM RATIO WARM:COLD max . mean min. mean - 28.0 82.4 24.3 75.7 - - - 35.05 34.33 34.71 34.29 - 8.35 8.02 8.24 7.55 - NO3 + NO2 u w t / '1* 0.50 0.05 0.17 39.62 1:230 NH4 wat/l 0.76 0.20 0.47 0.34 - p04 Si1 icon ug-at/l 0.34 0.03 0.15 3.00 1:20 vg-at/l 11.52 1.80 3.46 77.58 1: 22 Total Diss. Nitrogen ug-at/l 7.84 2.51 4.12 42.16 1: 10 Total D i ss. Phosphorus ug-atll 0.66 0.19 0.35 3.03 1:9 1.20 0.51 0.91 0.58 - Temperature ( i n Lab) Salinity PH "C O F O/OO Total Org. Carbon *microgram-atoms/liter 1286 make i t partic- - f l o w ,m e r e l yb yo p e n i n go rc l o s i n gv a l v e s ,p e r m i t s maintenance o f a n yt e m p e r a u r eb e t w e e nt h ei n p u t c o l dw a t e r( a l w a y sl e s st h a n 10°C)and t h ea m b i e n t a i rt e m p e r a t u r e( a l w a y sa tl e a s t 22°C).Temperai n t h i s way even i n open o r t u r e sc a nb em a i n t a i n e d s h a d e - c l o t h - c o v e r e dt a n k so r a c e w a y s N . o t et h a t b ym i x i n gt h ed e e pw a t e rw i t ha p p r o p r i a t ea m o u n t s oftheflowingsurfacewateralsoavailableat NELH, l a r g ev o l u m e so fs e a w a t e ra ta n yt e m p e r a t u r e between 10°C and 24°C (50°Fand75°F)canbeobtainedyear-round. 2 ) N u t r i e n t s - The h i g h n u t r i e n t l e v e l s o f t h e c o l d d e e pw a t e rp r o v i d et h eo p p o r t u n i t yf o rr a p i dg r o w t h r a t e s i n m a r i n ep l a n t s - e i t h e r f o r p l a n t p r o d u c t i o n o r as f o o d f o r d e s i r a b l e a n i m a l s . - 3) Purity S i n c et h ed e e pw a t e r comes f r o m w e l l below tine p h o t i c z o n e , v i a b l e p l a n t c e l l s a r e s c a r c e ,a l l o w i n gt h ec u l t u r eo fp u r es t r a i n so f a l g a ew i t h o u tc o s t l yf i l t r a t i o n .B a c t e r i aa n d o t h e rp a t h o g e nl e v e l s i n t h ed e e pw a t e ra r ea l s o e x t r e m e l yl o w ,p e r m i t t i n gc u l t u r eo fv e r ys e n s i t i v e a n i m a l a r v a ls t a g e s i n therawseawater. AQUACULTURE RESEARCH PROJECTS FOR OTEC BY-PRODUCTS B o t hr e s e a r c ha n dc o m m e r c i a la q u a c u l t u r ep r o ' e c t s havebeenconductedusingNELH'scoldwater.jThis s e c t i o nd e s c r i b e sr e s e a r c hp r o j e c t s ,i n c l u d i n g t h o s ew h i c hr e q u i r ev e r yl a r g ev o l u m e so fw a t e r , s u c ha sw o u l db ed i s c h a r g e df r o ma no p e r a t i n g OTEC p l a n t . Such p r o j e c t sm i g h tp r o v ev e r yp r o f i t a b l e i nc o n j u n c t i o nw i t ha n OTEC p l a n t evenenough t o s i g n i f i c a n t l yi m p r o v et h eo v e r a l le c o n o m i cv i a b i l i ty o f t h e OTEC process - b u t a l o n e t h e y m i g h t w e l l beunable t os u p p o r tt h et r e m e n d o u sc a p i t a li n v e s t m e n tr e q u i r e df o ri n s t a l l a t i o no ft h er e q u i r e dc o l d watersupplysystem. - T h en e x ts e c t i o n w i l d e s c r i b ec o m m e r c i a lp r o j e c t s w h i c hg e n e r a l l yu s el e s sw a t e rp e rp o u n do fp r o d u c t so t h a t , e s p e c i a l l y f o r i n t r i n s i c a l l y v a l u a b l e speon t h e i r c i e s , t h e y may p r o v e e c o n o m i c a l l y v i a b l e own. Such p r o j e c t se i t h e ru s es u c hs m a l l amounts o fw a t e rt h a tt h e yc a np r o d u c ec o m m e r c i a la m o u n t s o fp r o d u c tu s i n go n l yr e s e a r c hv o l u m e so fw a t e r ,o r t h ep r o d u c tv a l u ei sg r e a t enough so t h a t p i p e l i n e i n s t a l l a t i o n c o s t s become a c c e p t a b l e . SALMON AND TROUT - When t h e c o l d s e a w a t e r f i r s t became a v a i l a b l e e a r l y i n 1982, D r . A. F a s t o f t h e H a w a i iI n s t i t u t eo fM a r i n eB i o l o g y( H I M & )o b t a i n e d f u n d i n gf r o mt h e UH SeaGrant (UHSG) programandthe s t a t eD e p a r t m e n to fP l a n n i n ga n dE c o n o m i cD e v e l o p ment (DPED) t oc o n d u c t an i n v e s t i g a t i o n i n t o t h e p o s s i b i l i t yo fg r o w i n gs a l m o n a n dr a i n b o wt r o u ti n t h ec o l dd e e pw a t e r .T h i sp r o j e c tc o n t i n u e d t h r o u g ht h ee n do f1 9 8 4 ,a n di n d i c a t e ds i g n i f i c a n t p o t e n t i a lf o rg r o w i n gt h e s ef i n f i s hi nt h et r o p i c s . Rainbow t r o u t ( S a l m ok i s u t c h )w e r ea l r e a d yb e i n g g r o w ns u c c e s s f u l l yi nf r e s h w a t e ri nH a w a i i , and t h e ya d a p t e dw e l lt ot h ec o l ds e a w a t e ra t NELH. By m a i n t a i n i n gh i g hf l o w so fa e r a t e dw a t e r ,t h er e s e a r c h e r sw e r ea b l et ok e e pn e a r l y 400 l b s . o f f i s h a1 i v e i n each600-gal 1o n f i b e r g l a s s t a n k . Eggs werehatched i n f r e s h w a t e r a n d t h e fry r a i s e d t o thesmoltificationstage,afterwhichtheywere t r a n s f e r r e dt oc o l ds e a w a t e rw h e r et h e yg r e wr a p i d l y as " s t e e l h e a d " . E x p e r i m e n t sw i t hs a l m o n ,b o t h" K i n g "( C h i n o o k O n c o r h y n c u st s h a w y t s c h a )a n ds i l v e r( c o h o k i s u t c h ) , i n i t i a l l y g a v el o ws u r v i v a lr a t e s when thefishweretransferredfromfreshtosaltwater. R e c o g n i z i n gt h a te n v i r o n m e n t a li n f l u e n c e s on t h e smol t i f i c a t i o n p r o c e s s w e r e p o o r l y u n d e r s t o o d , D r . GordonGrau i n i t i a t e d a s e p a r a t ee x p e r i m e n tt o on s t u d yt h ee f f e c t so fp h o t o p e r i o da n dt e m p e r t u r e t h e smol t i f i c a t i o np r o c e s s .I n i t i a lr e s u l t si n d i c a t et h a tp h o t o p e r i o dh a sl i t t l ee f f e c t ,b u tt h a t lowertemeraturesdecreasethetimebefore smol tification. 0. 4 I nf u r t h e re x p e r i m e n t sw i t hm a t u r eb r o o dt r o u t ,t h e r e s e a r c h e r sm a n i p u l a t e dt h et e m p e r a t u r ea n dp h o t o p e r i o dt oc o n v i n c et h ef i s ht o spawn i n seawater. Theeggsandspermwerethen"milked"fromthe femalesandmalesandmixedtogether i nf r e s h w a t e r . These f e r t i l i z e d eggs e x h i b i t e d h i g h e r s u r v i v a l r a t e st h r o u g ht h es m o l t i f i c a t i o ns t a g et h a nh a d been o b t a i n e di np r e v i o u se x p e r i m e n t se l s e ~ h e r e . ~ NOR1 - A n o t h e r p r o j e c t i n i t i a t e d i n t h e s p r i n g of 1982 w i t h UHSG and DPED f u n d i n gg r e wt h e seaweed " n o r i "( P o r p h y r a %) u n d e rt h ed i r e c t i o no f D r . R. Spencerand F. Mencher.The n o r i ,w i d e l yu s e di n JapanandHawaii f o rw r a p p i n g" s u s h i " ,g r e ww e l l u s i n g a m i x t u r e o f warm s u r f a c ea n dc o l dd e e ps e a w a t e rR . e s e a r c h e r se x p e r i m e n t e dw i t hv a r i o u s temp e r a t u r e sa n dp h o t o p e r i o d s ,a n dc o n c l u d e dt h a t g r o w t hi sb e s tv e r yn e a rt h ec o n d i t i o n s i n the i n JapanG . r o w t hr a t e so f h a r b o r sw h e r en o r gi r o w s a b o u t 35% i n c r e a s e i n w e i g h t p e r d a y w e r e a c h i e v e d forthefirst 15days i n a t u m b l e - c u l t u r eu s i n g r a p i dw a t e rf l o wa n db u b b l e da i r .M o r et h a n 600gms drywgightpercubicmeterwereharvestedafter39 days.3Japaneseresearchersare now i n v e s t i g a t i n g c o m m e r c i a ln o r ic u l t u r ep r o s p e c t sa t NELH. MAINE LOBSTER - BeginninginSeptember1982, Sand e r sA s s o c i a t e s ,a ne l e c t r o n i c s companyheadquart e r e d i n Nashua, New Hampshire,performed a one yearpilottestoftheirproprietaryprocessfor g r o w i n gM a i n eL o b s t e r (Homarus a m e r i c a n u s )w i t h p a r t i a sl p o n s o r s h i pb y DPED. U s i n gm i x t u r e so f d e e pa n ds u r f a c es e a w a t e rt oc o n t r o lt e m p e r a t u r ea t d i f f e r e n ts t a g e so fh a t c h i n ga n dg r o w t h ,t h e ys u c c e s s f u l l yg r e wt h ea n i m a l sf r o m eggshatched i n H a w a i ti oa d u l t s .S a n d e r sd e t e r m i n e dt h a t h eh i g h costofthematerialsandthe manpower r e q u i r e d make t h e p r o c e s s u n p r o f i t a b l e a t t h e p r e s e n t l e v e l o fw h o l e s a l el o b s t e rp r i c e si nH o n o l u l u , so t h e p r o j e c t was t e r m i n a t e d a f t e r oneyear.6 OYSTERS - O y s t e r s( C r a s o s t r e a sp.) i m p o r t e d f o r a d e f u n c t p r o j e c t a r e now beinggrownby NELH s t a f f . I n i t i a la t t e m p t st oc u l t u r ev a r i o u ss t r a i n so f a l g a et of e e dt h eo y s t e r sm e tw i t hm i x e ds u c c e s s . R e c e n t l y ,r a p i dg r o w t hh a sb e e na c h i e v e db yf e e d i n g t h eo y s t e r sw i t hd i a t o m sb e i n gf i l t e r e df r o mt h e a b a l o n ef a r m ' sk e l pt a n k s .T h i ss u c c e s sh a s 1 ed t o t h ei m p o r t a t i o no fo t h e ro y s t e ra n dc l a ms p e c i e s f o rs i m i l a re x p e r i m e n t s . Though c o m m e r c i a l - s c a l e 1287 growoutof thesemollusks i n the tropics may require too much water and i n f r a s t r u c t u r e t o be economicallyviable, i t doesappear that the purity of the deep water may provideidealconditionsfor commercial hatcheryoperations. - Dr. Murray Dailey,incooFeration GIANT CLAMS w i t h the WaikikiAquarium, has begun a project w i t h private and grant funds u s i n g the deep water a t NELH to grow giant clams (Tridacna %) imported from Palau.Theseclams haveeconomic potential both f o r their meat and f o r t h e i r s h e l l s which, a t various stages of growth, have usesranging from earringstobathtubs.Sincetheysubsist on algae whichgrow symbiotically w i t h i n their bodies, they require no food beyond the sunlight and nutrients necessaryforplant growth. The i n i t i a l phase of t h i s p r o j e c t , beginning i n August 1985, includes research t o determine the e f f e c t s of thefollowing variables:(1)thenutrient-richwater, ( 2 ) colder temperatures,(3)flowrate,(4) l i g h t spectrum and ( 5 )s p a t i a ld i s t r i b u t i o n . CURRENT COMMERCIAL DEEP WATER AQUACULTURE PROJECTS ABALONE - In May 1984, HawaiianAbalone Farms (an a f f i l i a t e of MontereyAbalone Farmsof California) signedalong-termlease f o r 21.3 acres of land adjacent t o the NELH compound andbegan development of a "Commercial Demonstration Module" of an abalone(Haliotusreferens)productionfacility.This culminated more than two years of research a t NELH which indicated the suitability of the deep cold waterforabaloneculture. TheCompany has since moved i t s productionoperations fromMonterey to Kona and clearedthelandforthedemonstrationmodule. They arepresentlyinstallinga new coldwaterpipewith t h e i r own funding, and plan t o marketabalonesoon. Thiscompany's research hasdemonstratedtheuseful properties of the deep cold water for aquaculture of marinemollusks. The purity of thewaterpermits successfullarval growout w i t h o u t expensive waterpurification. The high l e v e l s of dissolved inorganic nutrients i n the deep waterproviderapid growth r a t e s and high proteincontent i n b o t h the microscopicdiatomsthemollusks consume as larvae which and i n the g i a n t kelp(Macrocystispyrifera) they eatasadults.Sincethe deep wateris always colderthanrequiredforthevarious growth stages, temperature can be maintained i n a l l systems by control1 ing water flow. MICROALGAE - Cyanotech Corporati on ofWoodinvi 11e , Washington has recently constructed large raceways on land leased a t NELH f o r t h e c u l t u r e of various marinemicroalgae. Initialproductionincludes Spirulina,aprotein-richmicroalga in g r e a t demand asahealth food supplement. Though Spirulina is normally grown i n freshwater, Cyanotech s c i e n t i s t s havedevelopedtechniques f o r growing i t i n NELH's pure nutrient-rich deep seawater. Thecompany is a l s o growing other microalgae w i t h very h i g h concentrations ofBeta carotene, a vitamin A precursor w i t h a recently-discovered ability t o preventcertaintypes of cancer.Futureplansincludedevel- 1288 opmentof other pharmaceuticalproducts croalgae. from m i - The microalqae grow i n oval raceways, some u p t o 550 f t 1 ong by50 f t wide, w i t h the water circulated by large paddlewheels.. The deepseawater i s intentionally warmed to the higher temperatures needed f o r optimum algal growth during i t s slow passagethrough thepipestotheraceways. Once production i n eachraceway i s i n i t i a t e d , t h e o n l y waterrequired is toreplaceevaporationlosses. The high nutrient content of thewater, i t s extreme purity and the h i g h sun1 i g h t a t Ke-ahole Point (the highest solar insolation measured a t sealevel i n the U.S.), combine toprovideideal growth condit i o n s f o r t h e s e rnicroalgae. COLD SEANATER AGRICULTURE! A t the instigation of NELH Board ChairmanJohn Craven, UH investigators have begun research on the possible use of the cold freshwater which condenses on thepipescarryingcoldseawatertoirrigate agriculturalcrops such asstrawberries. With funding from UHSG, several bedswere constructed a t NELH and plumbed withvariouscominations of coldwater p i p i n g r u n n i n g bothabove andbelow t h e s o i l . I n i t i a l r e s u l t s show t h a t s u f f i c i e n t w a t e r condenses from the ambient a i r a t Ke-ahole Point to provide adequate irrigation for strawberry growth. grown u s i n g this cold In addition,thestrawberries condensedwater consistently have about five times with the sugar content of control plants watered ambienttemperaturetapwater. Varying theflow r a t e of thecoldseawateralsoallowscontrol of the r o o t temperature of theplants,providingthe p o s s i b i l i t y of rapidseasonalcyclingforincreased f r u i t production. The i n i t i a l six-month p i l o t project conducted i n 1984 demonstrated the usefulness of the coldwater agriculture concept, and f u r t h e r work i s plannedsoon. FUTURE PLANS Recognizing the significant conunercial possibil it i e s developing from thecoldwaterresearch at NELH, t h e s t a t e government has begun developmentof the Hawaii Ocean Science and Technology (HOST) Park on 547 acres of land adjacent to the NELH property. Park s i t e s , ranging i n s i z e from three acres to 100 or more acres, will be leased to companies w i s h i n g t o takeadvantageoftheuniquenaturalattributes of Ke-ahole P o i n t for production of aquacultural, energy or related products. 7 I n i t i a l l y proposed by the HawaiiHigh Technology Development Corporation as an appropriate area of "High Tech" f o r Hawaii, the HOST Park has now receivedsupport from GovernorAriyoshi and the l e g i s l a t u r e . Design work is underway, and the infrastructure (including warm and coldseawater systems) will be completed in 1986. In addi tion t o the new p i pel ines planned f o r t h e HOST Park and f o r HawaiianAbaloneFarms, DOE i s presently contracting for final design of a new 30 i n . p i p e l i n ef o r OTEC research. Each of these pipe1 ines will give some measureofredundancy the NELH seawatersystem,providingtheincreased r e l i a b i l i t y which i s p a r t i c u l a r l y d e s i r a b l e f o r aquacultureresearch and development. to NELH willcontinuetoprovideresearch, development and pilot-scale "incubator" facilities for projects which may eventually expand i n t o t h e HOST Park f a c i l i t i e s . The capability of combining research with pilotscaleproduction makes NELH a unique site for important stages of commercial development. New ventures can now utilizetheseunique advantages, knowing t h a t f u l l - s c a l e devel oprnent may follow a t t h e HOST Park. ACKNOWLEDGEMENTS Chairman John Craven and the other unpaid members of the NELH Board are largely responsible for the success of thelaboratory. The work reportedhere hasbeenperformed bymany researchers andcompan i e s , a l l of whom d e s e r v e c r e d i t f o r t h e i r e f f o r t s . REFERENCES ILarsen-Basse, J . , e t a1 . , "ClosedCycle OTEC Corrosion and Heat Transfer Studies at the Seacoast Test Facility - Current Status", Oceans 85Proceedings, This volume. 2ResearchCorporation of the University of Hawaii, "OTEC SeacoastTestFacility - Preliminary System Design Description", Preparea for Argonne National LaboratoryContract No. 31-109-38-4406, February, 1980. 3Fast, A.W., e t a1 . , .'Xquacul t u r e Research a t NELH - 1981-1984",inpreparation by U H SeaGrant. 4Grau, E . Gordon, e t a l . , "VariationsinThyroid Hormone Levels and in Performancein theSeawater ChallengeTest AccompanyingDevelopment in Coho Salmon Raised inHawaii",presented atInternational Smol t i f i c a t i o n Workshop, sponsored by E.O.C., Stirling,Scotland, 3-6 July 1984, t o be published inAquaculture i n 1985. (Porphyra SMencher, F.M. , e t a l . , "GrowthofNori tenera) in an Experimental OTEC-Aquacul t u r e System inHawaii", J . World Maricul.SOC.,14:458-470, 1983. 6SandersAssociates,Inc/ResearchCorporation Univ e r s i t y of Hawaii,"LobsterAquaculture DemonstrationProject:FinalReport", December 1983. 7High Technology Development Corporation, "Hawaii Ocean Science and TechnologyPark:Marketing and FeasibilityStudy",Presentedtothe Hawaii S t a t e Legislature,January 28,1985. 1289
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