SelexION™ Technology
for TripleTOF® Systems
Site Planning Guide
RUO-IDV-01-0720-D | D5041245 D
March 2015
This document is provided to customers who have purchased AB Sciex equipment to use in the operation of such AB Sciex
equipment. This document is copyright protected and any reproduction of this document or any part of this document is
strictly prohibited, except as AB Sciex may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify,
or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the license
agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Warranties
are as stated therein.
Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whose
names are registered as trademarks and/or function as trademarks of their respective owners. Any such use is intended only
to designate those manufacturers' products as supplied by AB Sciex for incorporation into its equipment and does not imply
any right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks.
AB Sciex warranties are limited to those express warranties provided at the time of sale or license of its products and are
AB Sciex’s sole and exclusive representations, warranties, and obligations. AB Sciex makes no other warranty of any kind
whatsoever, expressed or implied, including without limitation, warranties of merchantability or fitness for a particular
purpose, whether arising from a statute or otherwise in law or from a course of dealing or usage of trade, all of which are
expressly disclaimed, and assumes no responsibility or contingent liability, including indirect or consequential damages, for
any use by the purchaser or for any adverse circumstances arising therefrom.
For research use only. Not for use in diagnostic procedures.
The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license.
© 2015 AB Sciex Pte. Ltd.
AB Sciex Pte. Ltd.
Blk 33, #04-06
Marsiling Ind Estate Road 3
Woodlands Central Indus. Estate.
SINGAPORE 739256
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Contents
Chapter 1 Introduction...............................................................................................................................4
Customer Site Planner Responsibilities..............................................................................................................................4
FSE Responsibilities............................................................................................................................................................5
Technical Support..............................................................................................................................................................5
Chapter 2 Site Planning Checklist..............................................................................................................6
Customer Information........................................................................................................................................................6
Requirements.....................................................................................................................................................................6
Site Layout Requirements............................................................................................................................................6
Electrical Requirements................................................................................................................................................7
Gas Supply Requirements............................................................................................................................................8
Ventilation and Waste-Collection Requirements..........................................................................................................9
Environmental Requirements.......................................................................................................................................9
Solutions and Equipment Requirements....................................................................................................................10
Product Familiarization..............................................................................................................................................10
Comments........................................................................................................................................................................11
Exceptions........................................................................................................................................................................11
Signoff.............................................................................................................................................................................11
Appendix A Site Requirements................................................................................................................12
Site Layout Requirements................................................................................................................................................12
Weights and Dimensions...........................................................................................................................................12
Installation Site Clearances........................................................................................................................................12
Electrical Requirements....................................................................................................................................................12
Mains Supply Connections.........................................................................................................................................13
Mains Supply Fluctuations.........................................................................................................................................13
Protective Earth Conductor........................................................................................................................................13
Uninterruptible Power Supply or Power Conditioner..................................................................................................14
System Electrical Specifications..................................................................................................................................14
Gas Supply Requirements................................................................................................................................................15
Optional Gas Generators...........................................................................................................................................18
Ventilation and Waste Collection Requirements..............................................................................................................18
Environmental Requirements...........................................................................................................................................20
BioSafety Requirements.............................................................................................................................................20
Sound Pressure Level.................................................................................................................................................20
Vibration....................................................................................................................................................................21
Customer Supplied Solutions and Equipment..................................................................................................................21
Appendix B Equipment Safety Categories..............................................................................................23
Revision History........................................................................................................................................24
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1
Introduction
This guide is for the site planner, the individual responsible for preparing the facility for the installation of the
TM
®
SelexION differential mobility separation technology device on the TripleTOF 5600+ or 6600 system .
The information in this guide supplements the information contained in the Site Planning Guide for the mass
spectrometer. The laboratory must meet the conditions detailed in both the mass spectrometer Site Planning
TM
Guide and the SelexION technology Site Planning Guide before the Field Service Employee (FSE) can install
the SelexION technology.
Customer Site Planner Responsibilities
Complete the Site Planning Checklist on page 6, in consultation with facilities services personnel (gas,
electrical, ventilation, and information technology [IT]), and return it to the FSE before the completion date. Refer
to Signoff on page 11.
Note: The FSE will follow up if the checklist is not received prior to the scheduled installation date.
• Verify that adequate space and the required shipping facilities are available. Refer to Site Layout
Requirements on page 12.
• Provide all required electrical receptacles. Refer to Electrical Requirements on page 12.
• Provide all required regulators, shut off valves, and gases. Refer to Gas Supply Requirements on page
15.
• Provide and install all required vents and ventilation devices. Refer to Ventilation and Waste Collection
Requirements on page 18.
• Verify that the requirements for the operating environment are met. Refer to Environmental Requirements
on page 20.
• Provide all required solutions and laboratory equipment, including all fittings, and sample tubing for the liquid
chromatography (LC) equipment, unless purchased from AB SCIEX . Refer to Customer Supplied Solutions
and Equipment on page 21.
• When the shipment arrives, inspect the packaging for damage and then contact AB SCIEX Customer Service
or the local FSE to schedule the installation.
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Introduction
FSE Responsibilities
• Review the checklist and discuss outstanding issues with the site planner.
• Test and qualify the system to the specifications in the Installation Checklist and Data Log.
Technical Support
AB SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout
the world. They can answer questions about the system or any technical issues that might arise. For more
information, visit the Web site at www.absciex.com.
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2
Site Planning Checklist
Customer Information
Organization
Address
City
Country
Telephone
Zip/Postal code
Site planner contact
name
Fax
E-mail address
Requirements
Site Layout Requirements
Refer to Site Layout Requirements on page 12.
Requirement
Complete
The measured building clearances can accommodate the equipment and crate dimensions.
If the requirements cannot be met, then contact the FSE.
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Site Planning Checklist
Electrical Requirements
Refer to Electrical Requirements on page 12.
Requirement
Complete
N/A
Installation of electrical supplies and fixtures complies with local regulations and safety standards.
TM
One branch circuit is provided for the SelexION technology. The SelexION
technology can be connected to the same branch circuit as the acquisition
computer.
The AC mains supply voltage source does not fluctuate more than ±10% from
the nominal voltage.
The mains supply includes a correctly installed protective earth conductor.
A qualified electrician has determined the appropriate AC mains supply
configuration based on the system electrical specifications found in System
Electrical Specifications on page 14.
Electrical Requirements (International)
Refer to Electrical Requirements on page 12.
Requirement
Electrical installations use locally approved standard connections and cables.
Complete
N/A
Complete
N/A
Electrical Requirements (North America)
Refer to Electrical Requirements on page 12.
Requirement
TM
The branch circuit for the SelexION technology is 15 A, 200 VAC to 240 VAC
(208 VAC typical), 50 Hz or 60 Hz. Receptacles for these branch circuits are
equipped with CSA/NEMA 6-15R straight-blade receptacles.
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Site Planning Checklist
Gas Supply Requirements
Refer to Gas Supply Requirements on page 15.
Requirement
Complete
N/A
Installation of gas supplies and connections complies with local regulations and safety standards.
Gas 1/Gas 2 is available:
• Zero-grade air or an AB SCIEX-recommended gas generator
• Delivery pressure of 105 psi (7.25 bar) maximum with flows up to 26 L/min
Source exhaust gas is available:
• Clean, dry, and oil-free air or UHP nitrogen (99.999%) or an
AB SCIEX-recommended gas generator
• Delivery pressure of 55 psi (3.79 bar) minimum with flows up to 25 L/min
TM
Curtain Gas supply is available:
• UHP nitrogen (99.999%) or an AB SCIEX-recommended gas generator
• Delivery pressure of 55 psi (3.79 bar) to 60 psi (4.14 bar) with flows up to
18 L/min
CAD gas is available:
• Clean, dry, and oil-free air or UHP nitrogen (99.999%) or an
AB SCIEX-recommended gas generator
• Delivery pressure of 60 psi (4.14 bar) maximum with flows up to 1 L/min
DMS Resolution gas supply is available:
• UHP nitrogen (99.999%) or an AB SCIEX-recommended gas generator
• Delivery pressure of 55 psi (3.79 bar) to 60 psi (4.14 bar) with flows up to
3 L/min
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Site Planning Checklist
Ventilation and Waste-Collection Requirements
Refer to Ventilation and Waste Collection Requirements on page 18.
Requirement
Complete
Installation of plumbing and ventilation fixtures complies with local biohazard regulations
and safety standards.
Ventilation of the laboratory environment in which the mass spectrometer will be used complies with local regulations and the air exchange rate is appropriate for the work performed.
A minimum of 10 air exchanges/hour is required.
A negative flow vent is provided with a total flow rate capacity of 283 L/min (10 cfm), as
measured at the inlet to the laboratory ventilation system.
A smooth fitting is provided for the roughing pump, with an outside diameter (o.d.) of 3.2 cm (1.25 inches). The vent is within 1.5 m (60 inches) of the exhaust port on the roughing pump
and at least 1 m (40 inches) above the floor.
A fitting is provided for the source exhaust drain bottle with an o.d. of 2.5 cm (1.0 inch). The vent is within 1.5 m (60 inches) of the source exhaust drain bottle.
Environmental Requirements
Refer to Environmental Requirements on page 20.
Requirement
Complete
The size of the laboratory is at least 31.7 cubic meters (1120 cubic feet).
N/A
An ambient temperature of 18°C to 25°C (64°F to 77°F) is maintained.
Over time, the temperature remains within a range of with the rate of the change
in temperature not exceeding 2°C (3.6°F) per hour. Ambient temperature
fluctuations exceeding the limits might result in mass shifts in spectra.
Note: Peripheral devices might have different operating environment
requirements. Confirm the operating environment requirements with the vendor
of each peripheral device that will be used with the system.
Relative humidity is 20% to 80%, non-condensing.
Air conditioning provides a minimum of 16 000 Btu/hr (mass spectrometer and roughing pump only).
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Site Planning Checklist
BioSafety Requirements
Refer to BioSafety Requirements on page 20.
Requirement
Complete
The site is not designated as BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).
Solutions and Equipment Requirements
Refer to Customer Supplied Solutions and Equipment on page 21.
Requirement
Complete
All required solutions and bottles are available.
Product Familiarization
Requirement
Complete
N/A
An account has been obtained on the AB SCIEX Learning Portal, and the online learning modules have been completed.
Go to www.training.absciex.com and click the link to request an account. When
you receive your account information, complete the online learning modules.
System documentation has been obtained and reviewed. Go to
www.absciex.com/userguides.
(Optional) A qualified maintenance person (QMP) who is suitably aware of the
electrical and chemical risks associated with servicing laboratory equipment is available for review of the service procedures with the FSE.
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Site Planning Checklist
Comments
Exceptions
Signoff
Site planner contact name
Completion date
(yyyy-mm-dd)
I acknowledge that all of the installation requirements, as specified in this document, have
been met.
FSE name
FSE e-mail
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Return date
(yyyy-mm-dd)
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A
Site Requirements
Site Layout Requirements
Return to checklist.
Weights and Dimensions
Equipment
Mass spectrometer
TM
SelexION
technology
Height
Width
Length
Weight
135.9 cm
(53.57 inches)
82.5 cm (32.5 inches)
140.8 cm
(56.5 inches)
458 kg (1010 lbs)
19 cm (8 inches)
66 cm (26 inches)
53 cm (21 inches)
13 kg (29 lbs)
Installation Site Clearances
Side
Clearance Required
Front, left, and right
1 m (39 inches) for service access
Back
0.7 m (28 inches) for service access
Electrical Requirements
Return to checklist.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the installation
of all electrical supplies and fixtures, and make sure that all installations adhere to
local regulations and safety standards.
TM
WARNING! Electrical Shock Hazard. Do not connect the SelexION technology to
the same branch circuit as either the mass spectrometer or the roughing pump.
TM
The total combined system (mass spectrometer, roughing pump, and SelexION
is 6960 VA (50 Hz or 60 Hz) at 240 VAC.
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technology) power consumption
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Site Requirements
Mains Supply Connections
TM
• Provide a branch circuit for the SelexION technology. The SelexION technology can be connected to the
same branch circuit as the acquisition computer.
• For installations outside North America, use locally approved standard connections and cables.
• For installation in North America, use a 15 A branch circuit equipped with NEMA 6-15R straight blade receptacles
(208 VAC or 240 VAC).
An external line transformer is not required.
Mains Supply Fluctuations
Return to checklist.
In areas where the mains supply is subject to voltage fluctuations exceeding ±10% of the nominal value (200 VAC
to 240 VAC), a power conditioner is required. High or low voltages can adversely affect the electronic components
of the mass spectrometer. Refer to Uninterruptible Power Supply or Power Conditioner on page
14.
Note: Peripheral devices might have different mains supply fluctuation limits. Confirm the mains supply fluctuation
limit with the vendor of each peripheral device that will be used with the mass spectrometer.
Protective Earth Conductor
Return to checklist.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protective
earth conductor. Any interruption of the protective earth conductor is likely to make
the installation dangerous.
The mains supply must include a correctly installed protective earth conductor that must be installed or checked
by a qualified electrician before connecting the mass spectrometer.
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Site Requirements
Uninterruptible Power Supply or Power Conditioner
Return to checklist.
TM
If the SelexION
specifications.
technology is powered by a UPS or power conditioner, then it must meet the following
Table A-1 UPS and Power Conditioner Requirements
Specification
Requirement
Output voltage
200 VAC to 240 VAC True on line (double conversion)
Frequency
50 Hz or 60 Hz
Waveform
Pure sine-wave
Minimum peak current
3 × nominal current
Output voltage distortion
< 3%
Output protection
Circuit breaker
Minimum power requirement
8000 VA
System Electrical Specifications
Specification
Value
Nominal input voltage
200 VAC to 240 VAC
Input voltage fluctuation
+/– 10% of nominal
Frequency
50 Hz or 60 Hz
Maximum input current
5A
Maximum input power
1200 VA
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Site Requirements
Gas Supply Requirements
Return to checklist.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use only
qualified personnel for the installation of all gas supplies and connections,
and make sure that all installations adhere to local regulations and safety
standards.
CAUTION: Potential System Damage. Regulate each supply separately at the mass
spectrometer to prevent over pressure damage to the mass spectrometer.
CAUTION: Potential System Contamination. Use compression fittings for gas line connections.
Do not use liquid pipe sealant. If threaded fittings must be used, then Teflon tape may be
used to seal the threads. Do not use soldered fittings unless the tubing is thoroughly
cleaned afterwards.
®
TM
The TripleTOF system equipped with SelexION technology requires specific gas flows. Use high-pressure liquid
nitrogen dewars or AB SCIEX-recommended gas generators.
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Site Requirements
Figure A-1 Recommended Gas Connections
Item
Gas
Description
Pressure
1
Gas 1/Gas 2
Zero grade air
Maximum 105 psi 26 L/min
(7.25 bar)
1/4 inch (0.64 cm)
2
Source exhaust
House air,
zero-grade air, or
N2
55 psi (3.79 bar) to 25 L/min
105 psi (7.25 bar)
1/4 inch (0.64 cm)
3
Curtain Gas
supply
N2
60 psi (4.14 bar)
18 L/min
1/4 inch (0.64 cm)
4
CAD gas
N2
60 psi (4.14 bar)
1 mL/min
1/4 inch (0.64 cm)
5
DMS Resolution N2
gas
TM
Flow Rate
(max)
55 psi (3.79 bar) to 3 L/min
60 psi (4.14 bar)
Tubing
1/8 inch (0.32 cm)
Note: Gas pressures and flow rates must be measured at the inlet to the mass spectrometer.
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Site Requirements
Note: Use UHP nitrogen (99.999%) or an AB SCIEX-recommended gas generator.
Note: Under some conditions, using air instead of nitrogen for source exhaust gas might improve sensitivity
and signal-to-noise.
®
Note: When using the NanoSpray ion source, do not use UHP N2 for Gas 1/Gas 2, as there is an increased
risk of corona discharge, which can damage the emitter tip.
Note: For CAD gas and the Curtain Gas supply, the input fitting on the gas and vacuum bulkhead is 1/4-inch
Swagelok, shown in Figure A-3. For Gas 1/Gas 2 and source exhaust gas, the input fitting is quick-connect,
shown in Figure A-2.
Figure A-2 Quick-connect Fitting on the Gas and Vacuum Bulkhead
Figure A-3 Swagelok Connection and Tubing
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Site Requirements
Optional Gas Generators
Return to checklist.
Gas generators are available from AB SCIEX. Refer to the manufacturer’s documentation that comes with the
generator for system specifications. Refer to the manufacturer's documentation for information on the number
and type of outlets required.
Ventilation and Waste Collection Requirements
Return to checklist.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Make sure
that the mass spectrometer is properly vented and that good general laboratory
ventilation is provided. Adequate laboratory ventilation is required to control
solvent and sample emissions and to provide for safe operation of the mass
spectrometer.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use only
qualified personnel for the installation of plumbing and ventilation fixtures,
and make sure that all installations follow local bylaws and biohazard
regulations.
CAUTION: Potential System Damage. Do not connect the source exhaust hose to the vent.
The connection must be made by a qualified FSE.
• Provide a ventilation system with a total negative flow rate capacity of 283 L/min (10 cfm) at item 1 in Figure
A-4 and a minimum of 10 air exchanges per hour.
• Provide a smooth fitting for the roughing pump, with an outside diameter (o.d.) of 3.2 cm (1.25 inches). The
vent must be within 1.5 m (60 inches) of the exhaust port on the roughing pump, and at least 1 m (40 inches)
above the floor. Refer to Figure A-4.
• Provide a fitting for the source exhaust drain bottle with an o.d. of 2.5 cm (1.0 inch). The vent must be within
1.5 m (60 inches) of the source exhaust drain bottle. Refer to Figure A-4.
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Site Requirements
Figure A-4 Vent Connections
Item
Description
1
Vent
2
Source exhaust hose: 2.5 cm (1 inch) inside diameter (i.d.)
3
Roughing pump exhaust hose: 3.2 cm (1.25 inches) i.d.
4
Source exhaust drain bottle. The bottle is shown outside of the bottle holder to illustrate tubing
connections. Make sure that the bottle is secured at all times to prevent spills.
5
Source exhaust drain tubing: 1.6 cm (0.625 inch) i.d.
6
Roughing pump vacuum inlet hose
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Site Requirements
Environmental Requirements
Return to checklist.
• Minimum room size of 31.7 cubic meters (1120 cubic feet)
• An ambient temperature of 18°C to 25°C (64°F to 77°F)
Over time, the temperature must remain within a range of 2°C (3.6°F) from the temperature at the time of last
calibration, with the rate of the change in temperature not exceeding 2°C (3.6°F) per hour. Ambient temperature
fluctuations exceeding the limits might result in mass shifts in spectra.
• Relative humidity from 20% to 80%, non-condensing
• Cooling and venting for the mass spectrometer and roughing pump: 16 000 Btu/hr
Note: Operation of the mass spectrometer at altitudes higher than 2000 m (6400 feet) above sea level might
impact mass spectrometer operation.
BioSafety Requirements
Return to checklist.
The site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). AB SCIEX does not install,
service, or repair AB SCIEX mass spectrometers in areas designated BSL-3 or BSL-4.
Sound Pressure Level
Return to checklist.
Sound Pressure
Value
TM
Average sound pressure level for the mass spectrometer, roughing pump, and SelexION
technology
69 dBA
Note: Sound levels are for the 5600+ or 6600 system without the optional enclosure.
A sound pressure level of 85 dBA above a reference sound pressure of 20 μPa is at present regarded by many
authorities as the threshold at which a hazard may be caused. Special means, such as the use of protective ear
pieces, can make a higher sound pressure level non-hazardous to the operator.
AB SCIEX recommends that the sound pressure level be measured or calculated by the user both at the operator's
position in normal use and at whatever point 1 m from the enclosure of the equipment has the highest sound
pressure level.
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Site Requirements
Vibration
Return to checklist.
• The packaged mass spectrometer was tested to confirm that the effects of transportation do not affect mass
spectrometer performance upon arrival at the customer destination.
• The mass spectrometer is designed to operate in any laboratory environment that accommodates precision
analytical instrumentation.
Customer Supplied Solutions and Equipment
Return to checklist.
WARNING! Toxic Chemical Hazard. Refer to chemical product Safety Data Sheets
and follow all safety guidelines when handling, storing, and disposing of chemicals.
In addition to the standard solutions required for the mass spectrometer, you must provide the following solutions
TM
for use with the SelexION technology.
• MS-grade acetonitrile
• MS-grade methanol
• MS-grade acetone (2 L)
• MS-grade isopropanol (2 L)
TM
Table A-2 Modifier Consumption @ 20 psi Curtain Gas
Modifier
Flow Rate (µL per minute)
Concentration
1.5%
3.0%
10%
Methanol
131.924
267.928
962.558
Acetonitrile
171.378
348.056
1250.425
Isopropanol
249.696
507.115
1821.856
Acetone
239.324
486.051
1746.182
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Site Requirements
Table A-3 Modifier Consumption @ 20 psi Curtain Gas Flow Rate (mL per hour)
Modifier
Concentration
1.5%
3.0%
10%
Methanol
7.915
16.076
57.753
Acetonitrile
10.283
20.883
75.025
Isopropanol
14.982
30.427
109.311
Acetone
14.359
29.163
104.771
Table A-4 Modifier Consumption @ 20 psi Curtain Gas Flow Rate (mL per 24 hours)
Modifier
Concentration
1.5%
3.0%
10%
Methanol
189.971
385.817
1386.083
Acetonitrile
246.784
501.201
1800.611
Isopropanol
359.562
730.245
2623.473
Acetone
344.627
699.913
2514.502
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B
Equipment Safety Categories
Description
Category
Equipment pollution degree
Pollution Degree 2
Mains supply transient overvoltage
Overvoltage Category II
Note: Environments with a Pollution Degree 2 rating include laboratories and sales and commercial areas.
For more information, refer to the International Electrotechnical Commission standards IEC 61010-1 and IEC 60364.
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Revision History
Revision Reason for Change
Date
A
First release of document.
B
Corrected the relative humidity range.
C
Updated for TripleTOF 6600.
January 2015
D
Added environmental and ventilation requirements.
March 2015
®
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July 2014
October 2014
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