OPEN ACCESS Int. Res. J. of Science & Engineering, 2014; Vol. 2 (5): 171-176 ISSN: 2322-0015 RESEARCH ARTICLE Bioelectrical Impedance Analysis and its Interpretation Gupta Swaroopa Rani N Department of Chemistry, Brijlal Biyani Science College Amravati, Maharashtra, India [email protected] Manuscript Details ABSTRACT Received : 15 May, 2014 Revised : 07 June, 2014 Re-Revised : 08 July, 2014 Revised received: 17 September, 2014 Accepted: 27 September, 2014 Published: 02 October, 2014 For ideal weight management and for a more accurate and precise body composition analysis full Body Sensing Technology Karada Scan Body Composition Monitor – HBF-375 is used which measures body composition- weight, body fat percentage, visceral fat level, subcutaneous fat and skeletal muscle percentage, RM, BMI and Body age. This analysis technique is based on Bioelectrical impedance method. The general principle behind BIA is that two or more conductors are attached to a person's body and a small electric current is sent through the body. The resistance between the conductors will provide a measure of body fat between a pair of electrodes, since the resistance to electricity varies between adipose, muscular and skeletal tissue. Fat-free mass (muscle) is a good conductor as it contains a large amount of water (approximately 73%) and electrolytes, while fat is anhydrous and a poor conductor of electric current. Each (bare) foot may be placed on an electrode, with the current sent up one leg, across the abdomen and down the other leg. ISSN: 2322-0015 Cite this article as: Gupta Swaroopa Rani N. Bioelectrical Impedance Analysis and its Interpretation, Int. Res. J. of Sci. & Engg., 2014; 2 (5):171-176. Key Words : Body age, Body fat, BMI, RM, Skeletal muscle, Subcutaneous fat, Visceral fat. INTRODUCTION Copyright: © Author(s), This is an open access article under the terms of the Creative Commons Attribution NonCommercial No Derivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is noncommercial and no modifications or adaptations are made. © 2014| All right reserved Bioelectrical Impedance analysis was used to establish their Nutritional Status. One hundred participants as in (Patterson and Shanholtzer, 2002) where measured using bioelectrical impedance analysis to assess the accuracy of total body water (TBW), extracellular water (ECW), and intracellular water (ICW). France et al. (2005) reported Thirty-two volunteer’s hydration status was measured using bioelectrical impedance analysis to monitor fluid shifts and cardiovascular function. Forty subjects were measured as in (Patterson and Spinks, 2005) using bioelectrical impedance analysis to assess their Total body water (TBW), intracellular body water (ICW), extracellular body water (ECW), this was then taken with blood pressure readings to assess if there is a correlation. As in (Farman et al., 2002) Two hundred and thirty seven adults were measured using bioelectrical impedance analysis to establish their body composition including their lean and fat mass in comparison to Echocardiographic Image Quality. Bahadori et al. (2003) studied one hundred and twenty subjects were recruited for this project, forty four of whom were obese. Bioelectrical impedance was used to measure the cohorts progress on the diet and effects on the lean body mass. 171 Gupta Swaroopa Rani N, 2014 Baker et al. (2005) studied twenty seven patients to see if Lean body mass, calculated by bioelectrical impedance analysis, could be used to more accurately drug dose in obese cardiac patients. As in (Maughan et al., 2005) measured the body fat of twenty eight subjects and compared different methods of body composition analysis for accuracy. Study (Fuller et al., 1993) is a comparison of various different bioelectrical impedance devices and formulas. Clewlow et al. (1998) twelve males subjects were used in their research project were bioelectrical impedance analysis was used to assess the effects of positive end-expiratory pressure on transthoracic impedance-implications for defibrillation. Durnin et al. (1994) thirty five elderly subjects were tested using bioelectrical impedance analysis to evaluate the use of equations on this population group. As in (El Habbal et al., 2005) ten jaundice babies body water was tested using bioelectrical impedance analysis to assess if this is reduced in this patient population. Paper (Bolton et al., 1998) looked at the feasibility on ten critically ill patients, of using bioelectrical impedance to monitor fluid shifts. Francaux and Poortmans (1999) twenty five male volunteers were measured using bioelectrical impedance to assess their intra cellular, extra cellular and total body water to show the effect of creatine on body composition. As in (Demeure et al., 2000) Bioelectrical Impedance was used to measure fourteen male subjects body composition pre and post exercise. In one of the pilot study (2001) Twelve volunteers were measured using bioelectrical impedance analysis to monitor the effectiveness of Slenernight supplements on their body composition. The aim of study (Barton et al., 2002) was to establish the effectiveness of Bioelectrical Impedance Analysis when measuring body composition in eighty five COPD patients. Guisado et al. (2003) by looking at the past history of patients aims to understand if this has any long term consequences on their body composition using Bioelectrical Impedance. This study used one hundred and four patients. Goran et al. (1997) studied Bioelectrical Impedance Measurements at Various Frequencies to Estimate Human Body Compositions. Patterson and Shanholtzer, 2003) the research, monitoring one hundred subjects, looked at how effective bioelectrical impedance is as a tool for psychophysiology research. As in (Harris et al., 2003) forty subjects were tested using bioelectrical impedance analysis to assess the lean mass in COPD patients. Research based of a mixed cohort of one hundred and sixty subjects (Ghosh et al., 1997). Of these roughly half were teenagers and half adults. This sample also included a proportion of patients with various medical disorders. The paper examines and compares Body stat’s handheld Bioelectrical Impedance devices against the Dual Energy X-ray Absorptiometry Device (DEXA). The purpose of this paper is to establish whether or not hand-held Bioelectrical Impedance Analysis can be used accurately to establish body composition accurately and in a timely fashion. Elia et al, (1994) applied a three-component model of body composition to a group of obese women. Using 29 172 surgical patients the research project (Hannan et al., 1995) aimed to establish the best method for calculating extracellular water and total body water. The study (Cowen et al., 1998) eighty nine subjects a mixed of healthy, and patients with GI malignancies, chronic liver disease and IBD. In present analysis of body composition such as Body Weight, BMI, Body Fat Percentage, Segmental Subcutaneous Fat & Skeletal Muscle Percentage (Whole Body, Trunk, Legs and Arms), Resting Metabolism, Visceral Fat Level and Body Age is done by Bioelectrical impedance technique and results are interpreted and corresponding instructions for better health improvement is given. MATERIALS AND METHODS For ideal weight management and for a more accurate and precise body composition analysis full Body Sensing Technology Karada Scan Body Composition Monitor – HBF-375 is used which measures body composition- weight, body fat percentage, visceral fat level, subcutaneous fat and skeletal muscle percentage, RM, BMI and Body age. The general principle behind Bioelectrical impedance analysis is that two or more conductors are attached to a person's body and a small electric current is sent through the body. The resistance between the conductors will provide a measure of body fat between a pair of electrodes, since the resistance to electricity varies between adipose, muscular and skeletal tissue. Fat-free mass (muscle) is a good conductor as it contains a large amount of water (approximately 73%) and electrolytes, while fat is anhydrous and a poor conductor of electric current. Each (bare) foot may be placed on an electrode, with the current sent up one leg, across the abdomen and down the other leg. There is little scope for technician error as such, but factors such as eating, drinking and exercising must be controlled since hydration level is an important source of error in determining the flow of the electric current to estimate body fat. The instructions for use of instruments typically recommended not making measurements soon after drinking or eating or exercising, or when dehydrated. Instruments require details such as sex and age to be entered, and use formulae taking these into account; for example, men and women store fat differently around the abdomen and thigh region. BMI and Ideal weight: Body mass index is defined as the individual's body mass divided by the square of his or her height. The formulae universally used in medicine produce a unit of measure of kg/m2. BMI = Weight (Kg) / [height (m)] 2 The WHO regards a BMI of less than 18.5 as underweight and may indicate malnutrition, an eating disorder, or other health problems, while a BMI greater than 25 is considered overweight and above 30 is considered obese. www.irjse.in Bioelectrical Impedance Analysis and its Interpretation The ideal BMI is 22. Maintaining an ideal weight can help prevent obesity or weight loss and other diseases, and lead a longer life. The ideal weight for BMI of 22 is calculated as follows. The release, and using, of energy in this state is sufficient only for the functioning of the vital organs, the heart, lungs, nervous system, kidneys, liver, intestine, sex organs, muscles, and skin. Ideal Weight (Kg) = 22 × [height (m)] 2 BMR generally decreases with age and with the decrease in lean body mass (as may happen with aging). Increasing muscle mass increases BMR. Aerobic fitness level, a product of cardiovascular exercise, while previously thought to have effect on BMR, has been shown in the 1990s not to correlate with BMR, when fat-free body mass was adjusted for New research has, however, come to light that suggests anaerobic exercise does increase resting energy consumption. Illness, previously consumed food and beverages, environmental temperature, and stress levels can affect one's overall energy expenditure as well as one's BMR. However this method of ideal weight calculation may not be applicable for professional athletes and body builders, who have higher muscles ratio in their bodies. Body fat percentage: Body fat percentage = [Body fat Mass (Kg) / Body weight (Kg)] × 100 Body fat includes essential body fat and storage body fat. Essential body fat is necessary to maintain life and reproductive functions. The percentage of essential body fat for women is greater than that for men, due to the demands of childbearing and other hormonal functions. The percentage of essential fat is 3–5% in men, and 10-16% in women. Storage body fat consists of fat accumulation in adipose tissue, part of which protects internal organs in the chest and abdomen. Visceral Fat: In humans, adipose tissue is located beneath the skin (subcutaneous fat), around internal organs (visceral fat), in bone marrow (yellow bone marrow) and in breast tissue. BMR (RMR): Basal metabolic rate (BMR), and the closely related resting metabolic rate (RMR), is the amount of energy expended daily by humans and other animals at rest. Rest is defined as existing in a neutrally temperate environment while in the postabsorptive state. In plants, different considerations apply. Skeletal muscle: Skeletal muscle is a form of striated muscle tissue existing throughout the human body, and which is under control of the somatic nervous system; that is to say, it is voluntarily controlled. It is one of three major muscle types, the others being cardiac and smooth muscle. As their name suggests, most skeletal muscles are attached to bones by bundles of collagen fibers known as tendons. Biological (Real) Age: Biological age is how time and lifestyle have affected organs and cells compared to other people of chronological age. Factors of biological aging include changes in the physical structure of the body as well as changes in the performance of motor skills and sensory awareness. Chronological age is current age in years, calculated from birth date. RESULTS AND DISCUSSION Table 1: Interpretation of Body Composition Report Body % Fat Gender Low Normal High Very High Female Up to 19.9 20-29.9 30-34.9 35 & more Male Up to 9.9 10-19.9 20-24.9 25 & more 0(Normal) +(High) ++(Very High) 0.5-9.5 10.0-14.5 15.0-30.0 Trunk Fat <15 Normal, 16-18 High, 18+ Very high Visceral Fat % Gender Skeletal Muscle % Female Male BMI Age –(Low) 0(Normal) +(High) ++(Very High) 18-39 < 24.3 % 24.3-30.3 % 30.4-35.3 % ≥ 35.4 % 40-59 < 24.1 % 24.1-30.1 % 30.2-35.1 % ≥ 35.2 % 60-80 < 23.9 % 23.9-29.9 % 30.0-34.9 % ≥ 35.0 % 18-39 < 33.3 % 33.3-39.3 % 39.4-44.0 % ≥ 44.1 % 40-59 < 33.1 % 33.1-39.1 % 39.2-43.8 % ≥ 43.9 % 60-80 < 32.9 % 32.9-38.9 % 39.0-43.6 % ≥ 43.7 % Underweight Normal Over weight Obese Up to 18.4 18.5-24.9 25-29.9 30 & more Int. Res. J. of Science & Engineering, 2014; Volume 2, No. 5, Sept. –Oct., 2014. 173 Case Male Female 1M 2 F 3M 4M 5F 6M 7M 8F www.irjse.in 9F 10 M 11 F 12 F 13 M 14 F 15 F 16 F 17 F 18 M 19 F 174 No. / Date Age Ht Wt Kg Fat % 28.11.12 14.11.12 29.11.12 09.12.12 90 76 76 76 149 149 149 149 48.5 63.2 63 62.9 37.8 45.5 46.5 44.1 Subcutaneous Fat % Whole Trunk Body 24.8 37.6 38.2 36.9 34.3 17.01.13 76 149 63.1 48.9 39.4 26.11.12 26.11.12 26.11.12 27.11.12 30.11.12 09.12.12 16.01.13 27.11.12 12.11.12 19.11.12 28.11.12 09.12.12 13.01.13 28.11.12 12.11.12 28.11.12 09.12.12 16.01.13 25.11.12 17.01.13 17.01.13 28.11.12 17.01.13 30.11.12 27.11.12 09.12.12 17.01.13 16.11.12 25.11.12 29.11.12 09.12.12 17.01.13 28.11.12 28.11.12 16.01.13 62 59 53 57 53 53 53 50 47 47 47 47 47 46 45 45 45 45 45 45 42 34 34 24 23 23 23 22 22 22 22 22 16 14 14 170 177 145 167 172 172 172 151 148 148 148 148 148 172 159 159 159 159 156 156 173 163 163 156 162 162 162 161 161 161 161 161 176 160 160 78.9 61.6 89.3 66 53.8 54.9 55.3 46.4 37.6 38 38.9 38.3 37.7 78.9 63.1 62.2 62.2 62.4 62.4 63.2 88.9 57.6 57.9 64.9 43.2 44.1 44.6 52.6 52.2 52.2 52 51.8 50.2 41 41.7 32.2 30.5 47.8 30.8 23.4 22.2 22.6 35.5 24.7 37.8 29.6 33.7 32.2 30.9 43.2 38.9 38 37.8 39.5 40.1 29.5 31.4 30.7 38.2 20.8 21.3 21.3 31.2 32.4 31.8 31.6 32.3 10.9 17.8 18.5 14.6 14.8 23.8 22.9 31.2 31.1 33.2 20.9 25.5 17.2 17.3 24.7 25 15.4 www.irjse.in 37.6 22.2 19.9 46.8 20.8 15.3 12.4 12.6 26.8 24 25.8 22 22 20.8 21.3 36.8 31.6 28.3 28.2 32.7 30.4 19.3 25.7 22 32.8 16.7 13.2 13.2 24.7 25.1 24.8 21.8 22.2 7.2 14.9 11 Legs Viscera l Fat % 57.3 50 10 15 14 14 Skeletal Muscle % Whole Trunk Body 20.3 17.9 17.5 18.5 12.5 - 56.2 15 16.6 16 5 30 11 3 3.5 3.5 4 2 2 2 2 2 13 12 7 7 7 8 8.5 16.5 4 3.5 7 1 0.5 0.5 3 2 2 2.5 2.5 - 26.8 28.9 17.6 27.4 31.1 31.3 31.2 22.1 22.3 21.3 23.4 22.3 22.6 28.5 19.5 21.8 22.2 22.3 21.4 21.2 29.5 25.3 25.6 22.5 29.2 29 29.1 25.6 25.3 25.5 25.6 25.3 38.9 30.9 30.4 Arms 20.3 20.6 20.2 20.5 44.5 42.8 33.6 32.3 51.5 51.1 45.2 44.9 54.3 27.5 48.4 27.9 42.9 37.4 32.8 32.7 28.4 28.4 45 45.8 38.9 39.3 30.1 26.9 11 Legs RM Kcal BMI Body Age 18.3 31.4 1175 1240 1233 1242 21.8 28.3 28.4 28.3 66 80 80 80 16.1 28.4 1223 28.4 80 1683 1431 1599 1491 1334 1355 1361 1037 908 906 939 920 914 1699 1254 1263 1269 1272 1263 1272 1868 1230 1237 1309 1041 1054 1063 1155 1145 1147 1145 1139 1334 1017 1025 27.3 19.7 42.5 23.7 18.2 18.6 18.7 20.3 17 17.3 17.8 17.5 17.2 26.7 28.8 24.6 24.6 24.7 25.6 26 29.7 21.7 21.8 26.7 16.5 16.8 17 20.4 20.1 20.1 20.1 20 16.2 16 16.3 65 46 80 55 34 35 36 47 34 37 36 36 35 55 60 55 55 55 57 58 57 38 38 43 18 18 18 26 26 26 25 26 - Arms 24.9 24.8 37.7 37.9 48.7 48.6 18 18.3 28.1 28.6 31 31.7 16.8 16.9 22.8 22.9 34.1 34.7 15.8 21.5 21.5 34.5 33.6 46.9 20.3 27.7 37.6 24.9 24.9 34.8 34.7 39.2 39.5 21.2 21 29 28.7 35.8 35.3 26.6 36.8 40.2 Gupta Swaroopa Rani N, 2014 172 Gupta Swaroopa Rani N, 2014 Table2: Different Body Composition Readings are as follows. Bioelectrical Impedance Analysis and its Interpretation Case No.1 Male Ideal weight can be calculated as 48.8 Kg and actual weight is 48.5 i.e. Normal. Fat 37.8 % very high. Trunk fat 24.8 very high. Visceral fat 10 % High. Skeletal muscle 20.3 % Low. RM 1175 Kcal, BMI 21.8 Normal. Body age 66. Case No.2 Female Ideal weight can be calculated as 48.8 and actual weight is 63.2 Kg i.e. 14.4 Kg over weight. Fat % is 45.5 very high. Trunk fat is 37.6 very high, Visceral fat 15 % very high. Skeletal muscle 17.9 % Low. RM 1240 Kcal, BMI 28.3 Overweight. Body age 80 high. Case No.3 Male Ideal weight can be calculated as 63.6 Kg and actual weight is 78.9 Kg i.e. 15.3 Kg over weight. Fat % is 32.2 very high. Trunk fat is 22.2 very high, Visceral fat 16 % very high. Skeletal muscle 26.8 % Low. RM 1683 Kcal, BMI 27.3 Overweight. Body age 65 high. Case No.4 Male Ideal weight can be calculated as 68.9 Kg and actual weight is 61.6 Kg i.e. 7.3 Kg under weight. Fat % is 30.5 very high. Trunk fat is 19.9 very high, Visceral fat 5 % normal. Skeletal muscle 28.9 % Low. RM 1431 Kcal, BMI 19.7 normal. Body age 46 low. Case No.5 Female Ideal weight can be calculated as 46.3 and actual weight is 89.3 Kg i.e. 43 Kg over weight. Fat % is 47.8 very high. Trunk fat is 46.8 very high, Visceral fat 30 % very high. Skeletal muscle 17.6 % Low. RM 1599 Kcal, BMI 42.5 very severely obese. Body age 80 high. Case No.6 Male Ideal weight can be calculated as 61.4 Kg and actual weight is 66 Kg i.e.4.6 Kg overweight. Fat 30.8 % very high. Trunk fat 20.8 very high. Visceral fat 11 % High. Skeletal muscle 27.4 % Low. RM 1491 Kcal, BMI 23.7 Normal. Body age 55 low. Likewise other cases can be interpreted. trunk fat , visceral fat, BMI and body age can be decreased. And has to undergo 29 month weight loss programme to lose 43 kg weight. Case No.6 Male In this case skeletal muscle % and RM has to be increased so that weight, body fat % , trunk fat and visceral fat can be decreased. And has to undergo 3 month weight loss programme to lose 4.6 kg weight. REFERENCES 1. Bahadori B, Brath H, Krejs GJ, Muchitsch R, Trinker M, Itz E, Yazdani-Biuki B, Zenz M, “Low Fat, Low Glycemic ndex Ad Libitum Diet Induces Weight Loss Preserves Lean Body Mass in Obese Healthy Subjects”, Presented at Nutrition Week, San Antonio, Texas, USA January 2003 2. Baker M, Mitchell I, Richens D, Shajar M, Skinner H, Skoyles J, “Calculation of Lean Body Mass Using Bio Impedance Analysis Could Be Used To Accurately Determine Heparin/Protamine Dosage For Obese Patients Undergoing Cardiac Surgery Using Cardiopulmonary Bypass”, Cardiothoracic Unit, Nottingham City Hospital Trust. http://www.bodystat.com/Corporate/ResearchPaper/VAL010.aspx Retrive on May 2, 2014. 3. Barton RL, Morgan MD, Singh SJ, Steiner MC, “ Bedside Methods Versus Dual Energy X-ray Absorptiometry for Body Composition Measurement in COPD”, Eur Respir J 2002 Apr; 19(4): 626-31 4. Bolton M, Brookes SJ, Campbell IT, Columb MO, Han, Nightingale P, “The Use of Multiple Frequency Bioimpedance to Assess Fluid Balance in Critical Illness”, Proceedings of the Nutrition Society, 1998, 53:62A 5. Clewlow Fb, Dalrymple-Hay MJRc, Deakin CDa, McLaren RMa, Petley GWb, “Effects of Positive End-expiratory Pressure on Transthoracic Impedance-implications for Defibrillation” Resuscitation, 1998, 37: 9-12 6. Cowen S, Ghosh S, Hannan WJ, Keller W, “Nutrition Index Determined By a Portable Multifrequency Bioelectrical Impedance Analysis Machine”, University Of Edinburgh, Western General Hospital. Edinburgh. GUT 1998; 42 (suppl 1), TW144-TW152, 7. Demeure R, Francaux M, Goudemant JF, Poortmans JR, “ Effect of Exogenous Creatine Supplementation on Muscle PCr Metabolism”, Int J Sports Med., 2000; 21: 139-145 8. Durnin JVGA, Murray LA, Reilly JJ, Wilson J, “Improve Estimation of Body Composition in Elderly Subjects by Use of Age-specific Prediction Equations”, The European Group for Research into Physical Activity for the Elderly II International Conference, Sept 1994, 9. El Habbal MH, Robinson D, Strehle E, “Body Water Is Reduced In Breastfed Jaundiced Babies”, King George Hospital, Ilford and The Royal Free Hospital, London. http://www.bodystat.com/ibweb/res/pdf/rs/15BodyWater%20Is%20Reduced%20in% CONCLUSION Case No.1 Male In this case skeletal muscle % and RM has to be increased so that body fat % can be decreased. Case No.2 Female In this case skeletal muscle % and RM has to be increased so that weight, body fat % , trunk fat , visceral fat, BMI and body age can be decreased. And has to undergo 10 month weight loss programme to lose 14.4 kg weight. Case No.3 Male In this case skeletal muscle % and RM has to be increased so that weight, body fat % , trunk fat , visceral fat, BMI and body age can be decreased. And has to undergo 11 month weight loss programme to lose 15.3 kg weight. Case No.4 Male In this case skeletal muscle % and RM has to be increased so that body fat % , trunk fat can be decreased and has to undergo 5 month weight gain programme to increase 7.3 kg weight. Case No.5 Female In this case skeletal muscle % and RM has to be increased so that weight, body fat % , Int. Res. J. of Science & Engineering, 2014; Volume 2, No. 5, Sept. –Oct., 2014. 175 Gupta Swaroopa Rani N, 2014 20Breast%20Fed%20Babies.pdf. retrieved on May 2, 2014. 10. Elia M, Fuller NJ, Sawyer MB. Comparative evaluation of body composition methods and predictions, and calculation of density and hydration fraction of fat-free mass, in obese women”, International Journal of Obesity, 1994; 18: 503-512. 11. Farman CA, MacDonald M, MacFadyen RJ, Smith CB, “Gender and Obesity Interactions in Echocardiographic Image Quality”, Echocardiography Unit, Raigmore Hospital, Inverness, .British Society of Echocardiography Birmingham Conference, UK, on 18th October 2002 [Abstract] 12. Francaux M, Poortmans JR. Effects of Training and Creatine Supplement on Muscle Strength and Body Mass, Eur J Appl Physiol., 1999; 80: 165-168. 13. France CR, Gill M, Patterson SM, Prause LM, “Hydration Status and Cardiovascular Psychophysiology”, Psychophysiology, 2005, 39, S64. 14. Fuller NJ, Sawyer MB and Elia M, “Use of a Threecomponent Model for Evaluation of Density and Hydration of Fat-free Mass and Validation of Body Composition Predicted from the Same Whole-body Bioelectrical Impedance Measurement, in Lean and Obese Women”, Abstracts of Communications 1993 (66A). 15. Ghosh S Meister D Cowen S Hannan WJ Ferguson A. Body Composition at the Bedside”, European Journal of Gastroenterology & Hepatology., 1997 Aug;9(8):783-8 16. Goran MI, Kabir I, Khaled MA, Mahalanabis D., “Bioelectrical Impedance Measurements at Various Frequencies to Estimate Human Body Compositions”, Indian J Exp Biol., 1997; 35(2):159-61 18. Hannan WJ Cowen S Plester CE Fearon KC deBeau A. Comparison of Bio-Impedance Spectroscopy and Multi-Frequency Bio-Impedance Analysis for the Assessment of Extracellular and Total Body Water in Surgical Patients”, Clinical Science,.(Lond) 1995 Dec;89(6):651-8. 19. Harris ML, Man WDC, Moxham J, Moxham J Man WDC, Mustfa N, Nikoletou D, Polkey MI, Rafferty GF, Soliman MGG, “Non-volitional Assessment of Skeletal Muscle Strength in Patients with Chronic Obstructive Pulmonary Disease”, Thorax, 2003; 58: 665–669 20. Maughan RJ, Ross JAS, Seaton A, Watt SJ, “Comparison of Three Methods for Assessment of Body Fat Content”. http://www.bodystat.com/ibweb/res/pdf/rs/11Maughan%20Aberdeen%20paper.pdf, Retrive on May 2, 2014. 21. Patterson S.M., Shanholtzer B.A., “Fluid Hydration Status Assessment in Behavioral Medicine Research: Seven-Day Test-Retest Reliability”, Annals of Behavioral Medicine, 2002, 24: S133 22. Patterson SM, Shanholtzer BA, “Use of Bioelectrical Impedance in Hydration Status Assessment: Reliability of a New Tool in Psychophysiology Research”, Int J Psychophysiol., 2003; 49 (3):217-26 23. Patterson SM, Spinks DE, “Relationship Between Hydration Enhancement and Blood Pressure: More Is Better”, Pyschophysiology, 2005; 39, S65 24. Pilot Study of the effect of a collagen hydrolysate supplement, Slendernight, on inch and weight loss”, Trial Dates: 1 June 2001 – 25 July 2001 (8 weeks) 17. Guisado JA, Peñas-Lledó EM, Vaz FJ, “History of Anorexia Nervosa Inbulimic Patients: Its Influence on Body Composition” International Journal of Eating Disorders, 2003; 34:148-155 176 www.irjse.in © 2014| Published by IRJSE
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