Botany Abstracts Judges, please do NOT pre-judge these abstracts. They should only be used to gain a better understanding of the projects you will see on State Science Day. SYST—Plant Systematics and Evolution Bethany Grace Cox Can the Aquaponic Process with Biochar be Used to Grow Soybeans in Sand? BOT MORP Sandy surface soils have low soil fertility, shallow root depth and erosion. There is concern about feeding a growing population using organics instead of man-made fertilizers. Aquaponics is an organic solution. Biochar, an organic compound, along with aquaponics benefits the soil. The combination can reduce need for man-made fertilizers. If aquaponics with biochar is used to grow soybeans in sand, then they will grow as well as those grown in aquaponics with biochar and soil. For this project, soybeans were soaked to begin germination. Four groups had ten pots each. Each pot had one seed. Control group contained 20% biochar and 80% potting soil. Sand group contained biochar and sand. Two aquaponic groups were lined with clay pebbles to prevent erosion. Ten pots contained the biochar mixture with soil, and ten pots contained the biochar mixture with sand. Each aquaponic system had a fish tank with ten goldfish and a water pump. Water was pumped through the aquaponic plants every twelve hours. Ammonia, pH, nitrite, nitrate, alkalinity, and hardness levels were tested. Control group recorded 598cm in stem height, 103 leaves, 27cm leaf width, and 38.4cm leaf length. Sand group recorded 626cm in stem height, 105 leaves, 30.5cm leaf width, and 39cm leaf length. Aquaponics with soil group recorded 797cm, 139 leaves, 35.2cm leaf width, and 47.4cm leaf length. Aquaponics with sand group had 792cm in stem height, 153 leaves, 35.3 leaf width, and 47.1cm leaf length. Water tests showed an ideal fish environment. Results confirmed the hypothesis. Madelyn Alice Schafer Can Acrylamide Affect Plant Life? BOT Morp Question is " Can Acrylamide Affect Plant Life?" Acrylamide is a chemical that causes cancer to animals. My hypothesis is that the will die because it is not a need of the plant. The basic things you need for the experiment is: acrylamide, soil, cups, seeds, and water. I filled the cups with soil and put acrylamide in some whereas I left some for controlled. The results showed that acrylamide helped the plant grow. This is very cool but I advise you not to eat any products grown with acrylamide. Alison Elizabeth Gossman Does Manure Help Grass Grow? BOT ECOL Farmers are everywhere in today’s society, but we don’t really mention their hard work as much as it should be. Farmers work hard every day to make people’s lives easier. For my experiment I tested which animal had the most beneficial fertilizer for plants. I tested cow, hog, horse, sheep and chicken manure, Botany Abstracts using water as my control group. My hypothesis was that the cow manure would have the highest average, because I got the sample from grass-fed, meat cattle, which eat more than dairy cattle. I predicted water would have the lowest average because the only time water is the only thing put onto crops is in small personal gardens. I made tea out of the kinds on manure and watered grass with the tea throughout two weeks of observation. My final results are as follows: hog, horse, sheep, cattle, chicken and then water. My hypothesis was proven half correct, because water did have the lowest average, but hog had the largest average. Jacob C Dennis MORP The Effect of Nitrogen on Glycine max Yields, Protein, and Oil Content BOT The objective was to compare Glycine max with nitrogen applied at planting to Glycine max without nitrogen applied at planting. With an ever growing world population, we must increase Glycine max yields to feed the world. Twelve alternating replications of Glycine max were planted, six with 150 liters per hectare of nitrogen applied using a 2X2 in-row application and six without nitrogen applied. Throughout the growing season, the replications were sampled randomly for population, DGCI, nodule count, and leaf analysis. At maturity, the Glycine max were harvested. At the end of each replication, the replication length, weight of the Glycine max, and moisture content was recorded. A sample was collected from each replication and was sent to a lab to be analyzed for protein and oil content. The average yield was calculated for each replication from the data collected at harvest. The average yield for the nitrogen replications was 4,164.61 kg/ha (61.97 bu/ac) and 4,070.63 kg/ha (60.57 bu/ac) for the no nitrogen. The data was not significant (p=0.496). The average protein content was 36.61% for the nitrogen and 36.86% for the no nitrogen. The data was not significant (p=0.24). The average oil content was 17.41% for the nitrogen and 17.62% for the no nitrogen. The data was not significant (p=0.37). The outcome may have been different if the growing season was wetter and if less nitrogen was applied. Additional research of nitrogen application on Glycine max needs to be performed to determine if it is beneficial. Julian Aaron Liber The Viability of Invasive Species for Use in Biofuels BOT PHYS Ethanol biofuels have become commonly used as a method to reduce greenhouse gas emissions from fossil fuels used in transportation. First-generation biofuels produced from food crops are better in this aspect, but still require land, water, and agricultural chemicals. Invasive species used to create secondgeneration biofuels from cellulose have possible benefits including minimal land and resource requirements. This paper detailed one aspect of viability of three selected invasive species for the production of cellulosic ethanol biofuel, as determined by their relative holocellulose content compared to Zea mays as a control. These species were Alliaria petiolata, Ailanthus altissima, and Phragmites australis. It was hypothesized that at least one of the species would be found to be more viable in terms of holocellulose content than the control. Collected plant material from each species was ground to form a powder, had extractives removed, and underwent delignification to determine holocellulose content gravimetrically. The results found A. altissima had the highest holocellulose content, with Z. mays, A. petiolata, and P. australis following respectively. Analysis of Variance (ANOVA) showed overall Botany Abstracts difference between the groups (F = 17.48, p < 0.0001). A. altissima and Z. mays were not significantly different (p=0.1097) as was A. petiolata and P. australis (p = 0.1028). All other relationships were statistically significant. This indicates that none of the species had significantly higher holocellulose composition than the control. A. altissima may be a good candidate for further investigation for use in producing cellulosic ethanol, because it was closely comparable to the control. Akul Ajit approach Rajan The phytotoxic effect of allelochemicals to help weed control in a natural BOT PHY Herbicides, only effective solution to control weeds can cause negative health effects and chemicals runoff into water pollutes the environment. I did this experiment to find a better solution than using herbicides. My background research showed that some plants produce natural herbicides. Such action and related chemical interaction is called Allelopathy. My science fair question: Is there a phytotoxic effect of allelochemicals that can help weed control in a natural approach? My first experiment investigated 1a) How will different allelopathic extract (Juglone and Basil) concentrations (100% and 50%) effect the weed? 1b) Is there a negative and positive allelopathic impact? My hypothesis was that the Juglone100% extract would have a stronger deteriorating effect. On the other hand, I hypothesized that Basil would have a positive allelopathic effect helping the weed growth. In my second experiment, I investigated how different weeds health would be affected by Juglone. My hypothesis was that the Juglone would massively deteriorate the condition of all weeds. In both experiments only part of my hypothesis was proved right. Juglone 100% deteriorated the condition of the weed leaves to 33% followed by the Juglone 50% (44%) then Basil 100% (53%) and finally Basil 50% (59%). This proved the phytotoxic ability of Juglone. Basil did not show a positive allelopathic effect though the weed did show germination. One weed i.e. Catsear faced no change, while the other 2 weeds deteriorated. This showed that despite the phytotoxity of Juglone, some weeds have tough fibers that stand up to Juglone. Cathryn Patricia Murphy The Effects of pH on the Growth of Raphanus Sativus BOT PHYS This experiment was formed to determine whether or not treating Raphanus sativus (radish) each day with substances of varying pH affected growth. The hypothesis in the experiment was: If pH is related to the growth of Raphanus sativus, then Raphanus sativus plants treated with basic or acidic substances will not grow as tall as those only treated with neutral substances. Each day, five groups of ten plants were treated, consisting of ten plants that were treated with high amounts of sulfur and another ten plants that were treated with moderate amounts of sulfur. The sulfur was then converted into acidic sulfuric compounds within the soil. Also included were a group of ten plants that were treated with high amounts of the basic chemical calcium carbonate (CaCO3) and another ten plants that were treated with moderate amounts of the substance. Finally, ten plants were treated only with neutral substances. Each of the plants’ growth and germination was measured every other day for twenty one days. After twenty one days, it was determined that Raphanus sativus plants treated with the highest amounts of Botany Abstracts acidic substances grew the least while those treated with only neutral substances grew the tallest. The results supported the hypothesis because the plants treated with neutral substances grew the tallest. Addyson Miller-Brown Paper Mill Sludge: A Usable Fertilizer BOT PHYS The question prompting this project is can paper mill sludge be used as fertilizer for plants and how does it affect the pH of substrates? Plants rely on nitrogen, phosphorous, potassium, water and other micronutrients for growth. Paper mill sludge is made up of these nutrients and holds more water than typical growing mediums. Paper mill sludge is also known to create a more neutral pH. Therefore, soybean plants grown in 50% paper mill sludge will grow larger and produce more beans than plants grown in 100% paper mill sludge, 75% paper mill sludge, 25% paper mill sludge, or the control. Also, the substrate in the 100% paper mill sludge will have a higher nutrient concentration of nitrogen, phosphorus, and potassium, and a more neutral pH than the 25%, 50%, 75% mixtures, and the control group. To investigate the hypothesis, seeds were planted in the five mediums and allowed to grow for 63 days. Neither hypothesis was supported. Results of this project show that plants grown in 25% PMS have more mass and have more bean pods on average. Finally, seeds planted in the 25% and 50% paper mill sludge groups germinated at a higher rate. All of the tests run on the groups to test nutrient content and pH came out the same. In conclusion, each hypothesis was incorrect. But paper mill sludge does impact the growth of soybean plants in a positive way. Natalie Anne Morman Heat's Effect on Radish Growth BOT ECOL This experiment involved planting radish seeds that have been exposed to different amounts of radiant heat and microwave radiation. The seeds were placed in the microwave for the following intervals of time; 15, 30, and 60 seconds. Other seeds were placed in the oven for 10, 30, and 60 minute intervals. The seeds were planted in a growing cell and measured over the span of ten days. It was hypothesized that the shortest intervals of time would grow best and no benefits would be shown through heat/ microwaves. The results showed that the hypothesis made was correct. The radish seeds that were microwaved for the least amount of time not only grew quicker, they also were greener. The control batch started sprouting before all of the others groups, naturally. The seeds microwaved for fifteen seconds were much greener than those of the other groups. They had a greater number of leaves as well as longer stems, shown through recorded measurements. In contrast, the seeds exposed to only fifteen minutes of heat in the oven, failed to sprout. Liliauna Carver How Does Air Quality Affect Lichen Growth? BOT ecol Have you ever been outside and seen little green crusty looking things on tree trunks or branches? Well those are called lichens. A lichen is composed of two or more dissimiliar organisms, algae and fungus that form a symbiotic relationship to produce a new vegetative body. In my research I found that lichens are good bioindicators, which means they can give us a good indication of the health of an environment. Botany Abstracts In my experiment I tested to see how air quality affects lichen growth, by soaking lichen disks in 6 different chemicals, I then placed the lichens that were in the chemicals in test tubes with 120 milliliters of water and 7 drops of methylene blue. In my results the lead nitrate, mercury chloride, and potassium chloride had the highest negative impact on the lichens. Methylene blue with water had the least negative impact on the lichens. Lindsey Ann Schneeg Gibberellic Acid and Bean Plants BOT Morph The purpose of this project is to find the correlation between amount and frequency of gibberellic acid being applied to soybean plants and the resulting increase in plant height and bean weight. The sampling included 56 soybean plants. They were separated into four sections with 14 individual plants in each section. Groups were labeled A,B,C and D. The A section was the control where no gibberellic acid was applied. Section B had the seeds soaked in gibberellic acid for 12 hours prior to planting. The recipe for this application (formula 1) was a solution of 15 ml of water combined with one scoop of gibberellic powder and mixed. Section C had formula 1 applied to it via sprayer every 2 weeks after the plant had sprouted. Section D was sprayed with formula 1 once a month after the seeds sprouted. Height measurements from the soil to the top of the stem for each plant was taken every 7 days and recorded. This experiment proved the hypothesis of this project, which stated that an increase in the amount of gibberellic acid applied to soybean plants will lead to taller stem growth and a higher yield. The experiment showed that on average, the plants in section C, which had the most gibberellic acid applied, had the tallest average bean stem height and higher weight. Jenna Rose Lapurga How Do Magnetic Fields Affect Plant Growth? BOT Plant Ecology The purpose of this experiment is to determine if the presence of a magnetic field has any noticeable effect on radish germination and development, and if so, what the effect would be. In 1862, Louis Pasteur was the first to study magnetism and its effects on plants. The Earth’s natural magnetic pull is called its geomagnetic field (GMF). Kinetic energy that converts into electrical conducting fluid is found in the Earths core, which is the reason for its magnetism. GMF’s and magnetic fields are powerful growth enhancers that have a profound effect on the growth of living organisms. This concept is called biogenetics (the scientific study of magnetic fields applied to living tissue). It is known that gravity affects plant growth by pulling on the plant’s protoplasm, causing a diamagnetic response, but little is known about magnetism and its effects on plant growth. The hypothesis for this experiment is that if a magnetic field is present near radish seeds, then those radishes will develop faster and healthier than radish seeds without a magnetic presence. Neodymium magnets (the most powerful permanent magnets ever made) were used to conduct the experiment. Ten of them were placed in the soil of cherry belle radish seeds, and other cherry belle radish seeds, the controlled group, were grown without a magnetic presence. The seeds with the magnetic presence germinated and developed faster than the Botany Abstracts controlled seeds, grew taller, and had better overall health, proving the hypothesis correct. Mason Bradley Howell Liquids That Grow Beans BOT The purpose of this experiment was to find out what liquids help a seed to sprout and grow. The hypothesis stated that if different liquids are tested then the result would be that tap water will grow a seed better than a seed tested with any other liquid. The procedures that were followed were placing a seed and a napkin into a plastic bag and pouring thirty millimeters of tap water into that bag. Measure how much the bean grows every two-three days. Then do this with the other liquids and compare the results. The results of the experiment were proven partially correct because carbonated water grew the seeds more efficiently instead of tap water. The results showed that carbonated water grew the seeds more productively than tap water, salt water, and vinegar. The results of this experiment are important because they show that carbonated water grows a seed better than tap water and salt water, and that vinegar does not grow a bean at all. Lima and soybean farmers can use this information in their everyday lives to know that carbonated water grows a seed/bean better and at a more efficient rate. Deloris Corcoran Does Carbonated Water Affect Soybean Growth? BOT ECOL Carbon dioxide (CO2) is a crucial element in the growth of plants. Plants use CO2 in the process of photosynthesis to produce plant sugars and needed food for growth. The purpose of this experiment was to determine if adding CO2 to the water used in the irrigation process of growing soybeans would improve the overall growth of the plants. The hypothesis was if soybeans are watered with CO2-infused water and well water, the soybeans with the CO2-infused water will grow taller and faster. To conduct this experiment, thirty-six soybean seeds were started in optimal growing conditions. Nine were watered with CO2-infused well water through a syringe, nine were watered with CO2-infused well water with a spray bottle, nine were watered with regular well water with a syringe, and nine were watered with regular well water with a spray. The results proved that the plants watered with CO2infused well water germinated faster and grew taller than the control group. The results proved the hypothesis to be correct. Cody Nathaniel Clark The Effects of Wind Stress on Glycine max BOT ECOL This project is a study of wind stress on Glycine max. The results from this project are important because we need to maximize crop yields. It was hypothesized that the average stem length and leaf surface area will decrease under wind stress. Soybeans were grown in both still air and in 8.4 mile per hour wind. The results showed that soybeans grew the best in the still air. After analyzing the results, it can be concluded that the hypothesis was supported by the data. The plants grown in wind were 17% shorter than those growing in still air. Leaves on plants growing under wind stress had 66% less surface area than those leaves on plants in still air. Applications for this research include increasing crop yields in areas where wind is frequent. It will be beneficial for farmers to install or plant natural windbreaks to reduce the impact of wind stress on their crops. Botany Abstracts VARSHINI ODAYAR Radiation Exposure: A study of the biological effects of electromagnetic radiation on plant growth BOT MORP Electromagnetic radiation from natural sources can harm human health and cause the dangers all around us. The purpose of this project was to discover the effects of these electromagnetic radiations and its biological response to living cells by using radish seeds as the test organisms. The initial prediction was that radiation would hinder the rate of germination and the early growth of the seedling. ,Ten seeds in each pot filled with 2 cups of soil were divided into 6 different groups and were exposed to the various sources(Wi-Fi, microwave, visible light, infrared, ultraviolet and x-ray).Each plant received the same amount of water and was placed under the same temperature on all days of the experiment. The seeds were heated for 1 minute in microwave radiation and irradiated seeds were used for x-ray radiation. The remaining seeds were placed under their respective radiation sources. ,Leaf appearance, stem development, germination rate and average height of the plants were recorded. The ultraviolet, visible light and infrared radiations affected the seeds positively by enhancing photosynthesis, chlorophyll and ATP production within plant tissues, while microwave radiation delayed germination rate, radio waves affected leaf development negatively, and X ray radiation stunted plant growth. The hypothesis was rejected, as radiation also benefited seed growth. In conclusion, radiation has its own positive or negative effects, whether hindering or promoting plant growth. ,Agricultural scientists can implement these results in horticulture for supplemental light sources. NASA can also use this concept for growing plants as food for long space explorations. NOWSHIN ISLAM Radiation Exposure: A study of the biological effects of electromagnetic radiation on plant growth BOT MORP Electromagnetic radiation from natural sources can harm human health and cause the dangers all around us. The purpose of this project was to discover the effects of these electromagnetic radiations and its biological response to living cells by using radish seeds as the test organisms. The initial prediction was that radiation would hinder the rate of germination and the early growth of the seedling. , Ten seeds in each pot filled with 2 cups of soil were divided into 6 different groups and were exposed to the various sources(Wi-Fi, microwave, visible light, infrared, ultraviolet and x-ray).Each plant received the same amount of water and was placed under the same temperature on all days of the experiment. The seeds were heated for 1 minute in microwave radiation and irradiated seeds were used for x-ray radiation. The remaining seeds were placed under their respective radiation sources. ,Leaf appearance, stem development, germination rate and average height of the plants were recorded. The ultraviolet, visible light and infrared radiations affected the seeds positively by enhancing photosynthesis, chlorophyll and ATP production within plant tissues, while microwave radiation delayed germination rate, radio waves affected leaf development negatively, and X ray radiation stunted plant growth. The hypothesis was rejected, as radiation also benefited seed growth. In conclusion, radiation has its own positive or negative effects, whether hindering or promoting plant growth. ,Agricultural scientists can implement these results in horticulture for supplemental light sources. NASA can also use this concept for growing plants as food for long space explorations. Botany Abstracts Meredith Suhr What is the most effective way to mechanically pollinate plants? BOT MORP Given the decrease in natural pollinators (i.e. bees and other pollinating insects) it is important to assess manual pollination methods. The purpose of this experiment was to examine which mechanical pollination method (artist’s brush vs. shaking) is the most effective for both compatible and incompatible fast plants. Results showed that the artist’s brush method was significantly more effective than the control group (no pollination) but was not different than shaking for the compatible plants while neither method was significantly more effective than the control group for the incompatible plants. Future studies should use bigger sample sizes and test these methods on produce plants. Aspen R Schneller Aquaponics Gardening BOT ECOL The aquaponics system’s purpose is to help plants grow two to three times faster than the hydroponics system, but there are different theories on what helps the plants grow. One theory is the fish manure will increase the amount of nitrogen in the soil which helps the plants grow. Also, the way the system is set up, without having soil, takes stress off the plants’ roots which allows the plants to grow faster. The purpose of my experiment is to find out if the aquaponics system will increase the amount of nitrogen in the soil and observe if the lettuce plants grow faster, compared to lettuce plants in the hydroponics system. My hypothesis is based on books I have read on aquaponics and data people have found, such as the Fresh Harvest Farm owners. To test my hypothesis, I set up two hydroponics systems, one system contained five goldfish (being the aquaponics system). I observed and recorded the height of the plants every day for three weeks at eight o’clock p.m. Also, every four days I tested the nitrogen and pH levels to support my hypothesis. I took a picture of each system and created graphs to support my data. The aquaponics system supported my hypothesis because the lettuce plants grew 2.76 times quicker than the plants in the hydroponics system. Also, the data taken for the nitrogen was three levels higher in the aquaponics system compared to the hydroponics system, resulting in the aquaponics system’s plants growing more efficiently. Maury Finn Tan Soak & Resolve the Bean Mystery BOT MORP The experiment that I’m doing is about: Can black beans soften faster or slower if they were soaked in vinegar, rubbing alcohol, or 1% low-fat milk, rather than just tap water? The beans will be soft enough if a toothpick can easily go through the beans. I did this analysis because my parents like making food with beans in it. I found out that a black bean is a good source of protein and fiber. It also has antioxidant and anti-inflammatory properties that are good for your body. Having known this information, I became curious on the process of cooking black beans. The packaging’s instruction states that the first step in cooking beans is to soak the beans in water overnight. I am trying to help my parents seek a much faster and efficient way to soften beans than soaking them overnight. This is the reason that I did this experiment. The data that I need is the time that it takes to soften beans by soaking it with the following substances: Botany Abstracts vinegar, rubbing alcohol, milk, and water. I need to find out if these substances can soften black beans faster or slower than tap water. The outcome was unexpected. I thought that the bean soaked in water will definitely soften the fastest because it doesn’t have as much fat, acid, and chemicals as the other liquids. It turned out that my hypothesis is wrong. Claire Kinnear Grafting Peas and Beans BOT MORP Grafting- a common gardening technique that unites a scion (top of one plant) with a rootstock (the root of another plant). Often times people graft their plants in order to repair a broken or injured plant, to speed up the growth by giving it a well developed rootstock, or to create a variety of plants in a safe way. The researcher wanted to test the quality of grafting among pea plants and bean plants to see if they would produce more fruit of higher quality, or if any crossing of the individual genomes would occur. The researcher hypothesized that grafted plants would grow faster, produce more, and be of better quality than the originals. To begin, the researcher planted 6 bean plants and 6 pea plants. After the plants were well developed, the researcher took 3 of each plant and crossed the tops of the peas and the bottom of the beans. Over the next 30 days, all of the plants were monitored and measured in height. Overall, the grafted plants had wilted and died, while the original plants grew at a healthy rate. What the researcher has concluded is that grafting is a difficult skill to master, and many methods could be tweaked to have a more favorable outcome. If the researcher had chosen plants with stems of more similar diameter, the plants might have lived. In conclusion, it is still an important experiment as it shows how delicate the lives of plants truly are, and only masters can manipulate them safely. Laurienna Sky Kurucz Plants and Proximity BOT SYST Will proximity between plants affect plant growth and health? Plants were planted with high, medium, and low proximities to other plants and tended. The plants with the highest proximity to other plants grew the slowest and had the highest number of seeds that did not sprout. Lauren Zipp The Biosorption of Heavy Metals by Invasive Plants of Ohio BOT PHYS Heavy metal water contamination is prevalent in the environment, and scientists are seeking alternative and more efficient water purification methods. Scientists have turned to biosorption, the use of natural materials to absorb metals from wastewater. This experiment extended last year’s project that tested phragmites’ and cilantro’s removal of lead. This year’s experiment tested phragmites and garlic mustard for the removal of lead, copper, iron, and zinc. The hypothesis stated that phragmites would absorb more zinc and iron, and garlic mustard would absorb more lead and copper. The results of the experiment did not support most of these predictions. The hypothesis was tested using a flame spectrophotometer to analyze the concentration of metals in a 1 or 10 ppm solution after four days of being in contact with dried and crushed plant material. Three trials were conducted for each metal and plant combination. The results were calculated using the Botany Abstracts prepared calibration standards and then compared to the corresponding 10 ppm metal control solution. Per average gram of plant, Garlic mustard absorbed a greater amount of lead, iron, and zinc, at 0.1740mg, 0.05211mg, and 0.06825mg, respectively. Phragmites absorbed more copper at 0.01106mg, compared to garlic mustard’s 0.09867mg. Garlic mustard absorbed the greatest amount of lead, averaging 0.1740mg lead per gram of plant (around 87%). These findings revealed that garlic mustard was more effective in the removal of the metals than phragmites, an exciting discovery that makes garlic mustard a promising candidate for the removal of lead from wastewater. Richard A. Pignatiello Ecology Which Fertilizer Will Produce The Greatest Yield BOT ECOL-Plant What fertilizer produces the greatest yield? In my project I used eight green peppers, eight yellow peppers, eight tomatoes, and eight sunflowers. I took two of each plant and stuck them in four separate sections: compost, poultry manure, synthetic fertilizer, and control to see which fertilizer produced the greatest yield. I originally had four manures in my proposal but had to remove one and change another. My hypothesis was cow manure would produce the greatest yield, but I changed manures and my hypotheses became compost/cow manure. However, I learned that not one fertilizer fits all. My hypothesis was not supported by my results. Poultry manure did the best in green peppers, synthetic fertilizer did the greatest in sunflowers and yellow peppers, and control had the best tomatoes. During the 2014 summer, there were a lot of bad storms and a few tornadoes. These storms knocked plants over more than once, and the plants had to be tied up. After my 100 days of growing, the garden literally collapsed due to a storm on day 101. In the end, synthetic fertilizer was the best overall, but all plants like something different and should be researched before fertilized. Kayleigh Queiser How Phosphates in Fertilizer Affect Algal Growth BOT Plant Ecology The purpose of my experiment was to discover the effect of different levels of phosphorous added into phosphate-free fertilizer on algal growth in different local water samples. I hypothesized that the phosphate-free samples would have half as much algal growth than those samples with levels of phosphorous. In 4 different samples of local water, tap water, Hudson Springs Park water with and without blue green algae added, and my backyard pond water (in 4 oz. samples in 8 oz. bottles), I added a small amount of blue green algae to each sample except for one set of Hudson Springs water. Next, I put the same amount of phosphate-free fertilizer into each sample, but left 4 controls. Then, I added 20 µg/L of the phosphorous solution in one of each sample, 40 µg/L in one of each sample, 80 µg/L in one of each, 160 µg/L in one of each, and then left one of each sample phosphate solution-free. After aerating, shaking and capping each bottle once a day for 2 weeks, the samples with 160 µg/L of phosphate solution had visibly the most algal growth. Madison Hildebrand Aquaponics versus Hydroponics BOT ECOL The problem is, ‘Do common leafy plants grow better in an aquaponic or hydroponic system?’ It is hypothesized that common leafy plants will grow better in an aquaponic system. A brief procedure of the experiment is as follows. One hydroponic system and one aquaponic system Botany Abstracts was made according to the directions given in the Procedure. Six of each type of plant were planted in each system. There were three different types of plants. Each plant was measured every other day and the results were recorded in a log book. My hypothesis was supported in some cases and rejected in others depending on the type of plant being grown. Anna K Whiting Classical Music and Cosmos BOT MORP People have often wondered if music affects plant growth; so an experiment was created. To do this experiment, one must place 20 cosmos seeds into each of 2 pots and give them water, playing classical music to one of them daily for 2 hours. The results of this experiment were that classical music causes the plants to grow taller and more plants sprout. To do this experiment more accurately, I would plant the flowers in May instead of December and add plants that are played jazz, pop, and rap music. These are the average and correct results. In conclusion, playing classical music to cosmos flowers will cause them to grow taller and more plants to sprout. Alexa Lillie Draper Pansies and the Perfect Light BOT MORP Plants usually grow outside. But what happens when they need to be protected from the cold of winter? My question is do artificial lights promote more plant growth than actual sunlight through a window? For example, if I want to start some seeds for plants inside, should I put them in a sunny window or should I place them underneath an artificial grow light? My hypothesis was that if plants are placed under an artificial grow light and in a sunlit window, then the plants under the grow lights will grow faster, because the grow lights will have more concentrated light that will help promote plant growth. In this experiment, I planted twenty pansy seeds on the same day. I placed ten pansy seeds under an artificial grow light and ten pansy seeds in a sunny window. All seeds were watered equally and measured on the same days. What I observed was that my hypothesis was in fact proven correct because all but two of the plants in the artificial light sprouted and all but four of the sunlit plants sprouted. I have come to the conclusion that if you want to start plants as seeds and you wish to start them inside, then you should purchase an artificial grow light to grow your plants under because it has more benefits for your seedlings than the regular sunlight that is coming through your window does." Anna M. Kruse Is Biochar a Viable Soil Amendment? BOT ECOL Many people want to add amendments to their soil to benefit their plants and improve the quality of their soil. This experiment investigated whether biochar is a viable soil amendment. It was hypothesized that the addition of biochar to soil would increase a plant's root growth and overall growth, as well as increasing the germination success rate, while decreasing the soil’s moisture and water retention capacity. Cool white florescent lights and fixtures, Arabidopsis thaliana seeds, seed starting trays, premium potting soil, 100% hardwood biochar, and water were used to test the hypothesis. In the Botany Abstracts experiment, soil mixtures with 0%, 25%, 50%, and 75% biochar were used to grow 25 plants each. The germination success rate was recorded, as well as the overall plant and root growth. Then the soil’s moisture and water retention capacity were tested and recorded. The hypothesis was partially supported. The testing group with 25% biochar showed a higher germination success rate than the soil with no biochar. However, when more than 25% biochar was added, the rate decreased compared to the control group. The same is true for overall plant growth. The portion of the hypothesis regarding root growth was supported because biochar was shown to steadily increase root growth. The hypothesis was also supported since biochar caused a steady decrease in soil moisture and water retention capacity. In cases of biochar over 25%, the soil was reduced to levels of moisture that were poor for plant growth. In this experiment, it was shown that the key to using biochar as an effective soil amendment is moderation. Hrishi Kousik Circadian clock in plants BOT Plant Morphology Ever experienced jet lag? Have you ever thought if plants could face the same thing? Well, that is what our project is all about. The purpose of our project is to prove that plants actually have a circadian clock, a mechanism that regulates all daily functions of life, similar to humans. We conducted experiments over six weeks to test the results. We used plants called Oxalis Triangularis, which, under normal day and night conditions, open their leaves during days and close their leaves during nights. In our experiments, we controlled the amount of light by closing the blinds and drapes during days to simulate the night conditions, and turning on all lights during nights to simulate the day conditions. We found that after a while the plant learns to adapt the flipped day-night cycle. We recorded the data on the outcomes from doing the experiment. We did this by noting the date,time,condition and the measurement of leaf opening. We did this for normal day and night-time and after switching the times. Our experiment worked out as planned. This discovery can help change our perspective of how plants adapt to their environments - which in-turn could throw light on whether light can be used as a growth enhancer. This becomes necessary with growing population and the need for organic healthy food. Grace Frances Fuchs Studying the Establishment of Viburnum lantana BOT ECOL The purpose of this research was to gain a better understanding of the establishment of Viburnum lantana, commonly known as the wayfaring tree. In 1997, nine shrubs were introduced at the USFS Research Laboratory in Delaware, Ohio. I hypothesized that the success of establishment was influenced by distance from the original planting and openings in the forest canopy. To test the hypothesis, a total of 300 individuals were GPS mapped across the property. All individuals were classified into groups based on visual estimates of height and stem count. Thirty individuals were more intensively sampled. They were considered representative of the entire population and chosen based on location, height class, and stem class. A poor relationship between height and distance form origin was found (R2 = 0.1274). Aging some of the shrubs showed that the original point of introduction was much earlier than originally thought. Therefore, no correlation between height and distance from the origin could be Botany Abstracts found. The population was found more frequently in mixed hardwood forest, which has more open canopy due to the death of ash trees. Since the invasive shrub Viburnum lantana has become well established, it poses a serious threat to local flora. Timmy Joseph Andrews Rates BOT PHYS Effects of a Hypobaric Environment on Plant Growth and Germination As we continue our dreams of conquering the frontier of space, we may one day wish to inhabit these new places. To do this, we will need to explore the possibilities of terraforming other celestial bodies to support human life. To this end, I tested the effects low pressure had on a plants’ ability to germinate and grow. I predicted that a hypobaric environment would retard the plants’ growth and germination rates due to lack of CO2 in the enclosure. To test this, I constructed a vacuum chamber, placed 18 seeds and 18 seedlings of corn into the enclosure, and reduced the pressure to ½ atm. For the next ten days I recorded the daily humidity and temperature, germination rate and time of each seed, and initial and final heights, along with the final biomass of the seedlings. I compared these results to a control enclosure which had the same set-up, but remained at atmospheric pressure. From this data, I discovered that both the germination and growth rates were, in actuality, positively affected by the lower pressure. More seeds germinated and it occurred earlier in the hypobaric environment. The seedlings also experienced a higher growth rate as compared to the control, as their average biomass and final height were both higher than the control. With this data, I can conclude that a plant can germinate and grow in a hypobaric environment, and at a better rate than atmospheric pressure, which gives a very positive outlook to the future of terraforming. Sierra E Wentz Water Quality: What type of dung makes duckweed grow the most? ECOL Plant Ecology BOT The purpose of this project was to figure out what type of dung makes duckweed grow the most. My research showed that people grow duckweed to harvest and to eliminate algae problems in ponds. In six different containers, I put 20 duckweed in 6 cups of water. I put 1 Tablespoon of dung in each container except one container, which was the control. Each container had a different type of dung, including rabbit, horse, dog, duck, and chicken. I counted how many duckweed were in each container weekly. I thought that the duck dung would make the duckweed grow the most. The dog dung produced the highest amount of duckweed (an average of 52) over seven weeks and the horse dung produced the lowest (an average of 9). Carolyn Cox The Future of Feces: Fertilizer Effectiveness BOT ECOL Feces is used a lot by farmers, so this project could be valuable in helping farmers grow better crops. The purpose of this experiment was to find out what type of feces would grow plants the tallest. It was hypothesized that if Lima beans are planted in rabbit feces, chicken feces, horse feces, cow feces, and pig feces then rabbit feces will grow the tallest plants, because rabbit feces has a higher level of Botany Abstracts nutrients. Two seeds were planted in starters filled with each type of feces and measured biweekly. The data was analyzed and the conclusion was drawn that the rabbit feces grew the tallest plants at 116.00 mm, proving the hypothesis correct. Rabbit feces has high levels of nitrogen and phosphorus, both of which are known to cause rapid growth and reproduction in a plant. Karlie Louise Vandeborne Germination? BOT ECOL How Does Temperature Of A Soy Bean Seed Before Planting Affect The hypothesis of this project is that the room temperature seeds will germinate first because they were not exposed to any extraordinary temperatures. In this experiment, nine seeds were placed in a freezer at -20°C, nine seeds were placed in a heating pad at 45°C, and nine seeds were kept at room temperature at 22°C for twelve hours. Then the seeds were planted in 0.5L of soil and watered with 100ml of distilled water every other day for ten days. Germination was recorded. The results of this project proved that the room temperature seeds did not germinate before the other seeds. The room temperature and frozen seeds germinated on the same day for all three trials. It was also proven that the heated seeds were most affected by their temperature because heating the seeds increased the moisture levels and inhibited germination. This experiment proved the hypothesis incorrect. A cool, dry environment with moisture levels around 10% is best for seed storage." BRADLEY CHAO Investigation of Circadian Rhythms of Plants BOT SYST Ever experienced jet lag? Have you ever thought if plants could face the same thing? Well, that is what our project is all about. The purpose of our project is to prove that plants actually have a circadian clock, a mechanism that regulates all daily functions of life, similar to humans. We conducted experiments over six weeks to test the results. We used plants called Oxalis Triangularis, which, under normal day and night conditions, open their leaves during days and close their leaves during nights. In our experiments, we controlled the amount of light by closing the blinds and drapes during days to simulate the night conditions, and turning on all lights during nights to simulate the day conditions. We found that after a while the plant learns to adapt the flipped day-night cycle. We recorded the data on the outcomes from doing the experiment. We did this by noting the date,time,condition and the measurement of leaf opening. We did this for normal day and night-time and after switching the times. Our experiment worked out as planned. This discovery can help change our perspective of how plants adapt to their environments - which in-turn could throw light on whether light can be used as a growth enhancer. This becomes necessary with growing population and the need for organic healthy food. Madeline McFarland Which Weed Killer Works Fastest? BOT ECOL The purpose of this project was to see which weed killer worked the fastest. Weed killers that were tested were Roundup Weed and Grass Killer, Spectracide Weed and Grass Killer, Vinegar, and boiling water. The hypothesis was, ‘If 25 radish plants, 5 control plants and 20 with various weed killers, are Botany Abstracts planted and grown, then the radish plants with weed killer #1 which is Roundup Weed and Grass Killer, will kill the radish plants the fastest.’ The procedure was done by planting 50 radish cups. After the plants were all grown, the 25 plants that looked the best were chosen to be sprayed with weed killer; five with Roundup, five with Spectracide, five with vinegar, and five with boiling water. The results showed that Roundup worked the fastest and prevented the weeds from growing back. In conclusion, the hypothesis was correct. ALLISON DREW MIZER WHICH WORKS BEST FOR RADISH PLANT GROWTH? BOT MORP The purpose of this experiment was to find out if the synthetic fertilizer, Miracle Gro, would cause seeds to sprout quicker and the plants to grow taller than those receiving no fertilizer. The claim of this product is that ‘Miracle-Gro will grow bigger, more bountiful plants’. This experiment was done using twenty-four radish seeds. Half received Miracle-Gro and half received distilled water only. The data was obtained by observing and recording the exact day each seed sprout appeared. As the plants grew, the height of each was measured and recorded at the same time of day for twenty-eight days. After three separate trials, the results showed that the twelve seeds fertilized with Miracle-Gro sprouted an average of 0.3 of a day quicker than the twelve without fertilizer. The data results for the average maximum height of the radish plants showed that the twelve plants without Miracle-Gro actually grew fifteen millimeters taller than the twelve with Miracle-Gro. Benjamin Thomas Genetic Modification, Water, and Soybean Performance BOT PHYSIO The question that I researched was: Does the amount of water given significantly affect average plant height after twenty-one days in genetically modified versus wild-type soybeans? Before experimenting, I had to research the topic of genetic modification and how to grow soybeans indoors. I tested this by arranging eighteen six-pot cells in two rows of nine under a grow light, with the left six being A with 0.75 inches of water every other day, the middle six B with one inch, and the left six C with 1.25 inches. The back three cells in each group had wild-type seeds and the front three GM seeds. This explains the subgroup names (group C wild type: Cw). Over twenty-one days I took data and averages for each cell for average plant height, seeds germinated and mass of biological material and water. My hypothesis was that if my procedure was followed, then subgroup Cg would have the highest values in the three dependent variables. It would be followed by Bg, Ag, Cw, Bw, and Aw because of the advantage of more water and the superlative genetic advantage. The order of subgroups in height and germination was Cw, Bw, Aw, Bg, Ag, then Cg. This could be because the GM plants were adapted to different conditions or the seeds were old/a bad batch. The plants had little effect on mass. The field of genetic modification has applications including biofactories and increasing hardiness and yield in plants." Alexander Sawchuk Optimizing Algae Growth In A Photobioreactor BOT MORP The purpose of doing this experiment was to find the ideal growing conditions for algae in a photobioreactor. The hypothesis in this experiment was that the algae grown in a nutrient solution, placed in a 72-degree environment and exposed to 12 hours of light and 12 hours of dark every day Botany Abstracts would grow the best when compared to the other conditions. Theprocedute of this experiment involved building 4 mock photobioreactors out of 50ml test tubes and test tube racks. Then the test tubes were filled with either water or nutrient solution as well as 2ml of the algae solution. Then the four photobioreactors were placed in different conditions to see which condition caused the algae to grow more. The hypothesis was confirmed, the algae being grown in nutrient solution, placed in 72degree temperature, and were exposed to 12 hours of light and 12 hours of dark grew the best out of all the groups. Francesca Boni ECOL Which Grows Lettuce Faster: The Hydroponics System Or Regular Soil? BOT This project is titled ""Which Grows Lettuce Faster: The Hydroponics System Or Regular Soil?"" The hypothesis of this project is that the lettuce plants in the hydroponics system will grow faster each week because of the continuous water supply the plants receive. In this experiment, thirty lettuce plants are planted in soil and watered with water and thirty are planted in rockwool with a nutrient solution. The plants are compared in height over time. This project demonstrated the different methods of plant growing. It also demonstrated the rapid growth rate of hydroponics. The research also found that the hydroponically grown plants mature 30% to 50% faster and yield more than the soil grown plants for multiple reasons. One being the nutrients sent straight to the roots and not indirectly to the soil. The hydroponic plants also use very little energy to break down food. The experiment showed that the hypothesis was partially correct because the hydroponics did grow faster than the soil grown plants, but not just because of the continuous water supply. Aubree Ann Arlene Houin When Less is More: The Effect of Reduced Planting Rates on Ohio Soybean Yields and Profitability BOT PHYS My science fair project is titled, ‘When Less is More: The Effect of Reduced Planting Rates on Ohio Soybean Yields and Profitability.’ My objective was to determine if reduced soybean planting rates would result in higher yields and/or greater profitability in Ohio soybean fields. I selected this project because I wanted to learn more about soybeans and how farmers can increase their profitability on this important Ohio crop. My hypothesis was that modern agricultural production methods allow farmers to significantly reduce traditional soybean planting rates and populations without sacrificing yield, therefore improving on-farm profitability. I believe the reduced input cost from reducing seeding populations coupled with improved yields will result in greater profitability for Ohio soybean farmers. I believe reduced planting populations will provide greater space per plant and allow plants to utilize their energy for developing beans rather than vertical growth caused by competition for sunlight. Controlled variables were the soybean variety, weather, planting date, tractor operator, tractor and planter, combine, yield monitor, AgLeader Integra unit, 15-inch row spacing, and soil fertility. Measured variables were seeding population rate, yield, soil type, and management zones. Yield was measured as bushels per acre measured through yield monitor in combine and analyzed on AgStudio GIS software. Upon completion of my project, I learned we can continue to reduce soybean planting populations in Ohio soybean fields without negatively affecting yield or income." Botany Abstracts Benjamin Carter Fox Spradling Water vs. Soil BOT ECOL-Plant Ecology Hydroponic growing has come to be a hot topic because of the demand for clean, organic, and sustainable growing. For this project I had four different environments and place three philodendron plants in each environment to see which one grew the tallest. The four different environments that I used were Water, Water with Fertilizer, Soil, and Soil with Fertilizer. I measured the plants to find how tall they were every two days in each environment. My hypothesis was that Water with Fertilizer plants would grow the tallest. The hypothesis was not supported by the results because the water plants were the tallest in the end. This experiment proves that growing philodendron plants hydroponically is the best way to get the biggest growth. Sarah Lena Kikel contaminated soil Using Wisconsin Fast Plants (Brassica rapa) to phytoremediate zinc BOT ECOL Heavy metal pollution in soil and water is a worldwide environmental problem. Phytoremediation, the use of plants to clean up the environment by absorbing heavy metals in soil, water, or air, has been previously used to eliminate pollutants in contaminated areas. Wisconsin Fast Plants (Brassica rapa) is a common field mustard which has a rapid life cycle and can be grown under a variety of conditions. This plant could be a practical, cost effective tool for soil clean up. The purpose of this experiment was to determine Brassica rapa’s ability to phytoremediate zinc in artificially contaminated soils. Plants were grown in soils contaminated with 6.54 ppm (low), 65.4 ppm (medium), and 654 ppm (high) of zinc. After contamination, all plant group height averages continued to increase until day 25, where the medium and high groups decreased slightly. After determining the specific amount of zinc in the plant material, it was found that all the contaminated plants had absorbed some of the zinc. The amount of zinc in each plant group increased as the contamination levels increased. Under these specific conditions Brassica rapa is able to absorb zinc. Francisco garabis caffeine effect on plant growth BOT morp growth of plants using caffeine Kyle James Peck A Plant's Best Friend: Fish or Fertilizer? BOT ECOL The purpose of this experiment is to find out if a fish feces water mixture and a liquid manmade fertilizer added to different soils can change the way plants grow. The experiment began with watering soybean plants with either an organic fish feces mixture or a manmade fertilizer mixture. Then, the plant's growth and overall healthiness was observed and recorded every few days over a period of time. This experiment was conducted two times using a new set of seeds, pots, and fertilizers each time. The amounts of sunlight, water, and temperatures were the same for all the plants throughout the experiment. Botany Abstracts The hypothesis is that the soil fertilized with the fish feces water mixture will have the greatest effect on the plant's growth. The results showed that the plants in the soil fertilized with the fish feces water mixture had the overall largest amount of growth. Therefore, the hypothesis was proven correct." allison Nicole frank Maple Tree Sap BOT ecol Research Question, The research question is ‘ which trees produce the most sap?’ Hypothesis, I believe that trees with a larger circumference will produce more sap because the tree will have more storage for the sap. Procedure, I measured the circumference of the trees and counted the branches on the maple trees. My father and I then drilled holes in the trees and tied empty water jugs on the tree to catch the falling sap. Every day I went outside and measured how much sap the trees produced. Conclusion, The results indicate that my hypothesis was incorrect. My results were inconclusive, but I noticed that trees #10 - #15, which face south west, produced the most sap. Sophia Paul Increasing Soybean Germination Rate BOT MORP Seed germination begins with the rupturing of the seed coat and the emergence of the radicle, which will form the first roots of the seedling. Molybdenum is a catalyst for several enzymatic reactions in a cell, and potassium chloride is an essential macronutrient for plants. Gibberellic acid stimulates cell division, and polyethylene glycol lowers osmotic potential of cells. The experiment tested the germination rate of soybean seeds in molybdenum, potassium chloride, gibberellic acid, polyethylene glycol, and distilled water solutions. The hypothesis was that if gibberellic acid stimulates cell division and decreases dormancy, then a higher percent of the soybean seeds in the gibberellic acid solution will germinate than in the other solutions. 48 soybean seeds were germinated on cotton pads soaked in each solution for ten days. A similar percentage of seeds in the molybdenum, potassium chloride, gibberellic acid, and distilled water solutions germinated by the end of the duration of the experiment. Fewer seeds in the polyethylene glycol solution germinated, and the radicles of the germinated seeds in the polyethylene glycol solution were significantly shorter than the radicles of the seeds germinated in other solutions. The radicles of the seeds in the potassium chloride solution were longer than the radicles of seeds in the other solutions. Katherine Lynn Gothard Increasing Germination Rate of Glycine Max Seeds BOT ECOL Seed germination begins with the rupturing of the seed coat and the emergence of the radicle, which will form the first roots of the seedling. Molybdenum is a catalyst for several enzymatic reactions in a cell, and potassium chloride is an essential macronutrient for plants. Gibberellic acid stimulates cell division, and polyethylene glycol lowers osmotic potential of cells. The experiment tested the germination rate of soybean seeds in molybdenum, potassium chloride, gibberellic acid, polyethylene glycol, and distilled water solutions. The hypothesis was that if gibberellic acid stimulates cell division and decreases dormancy, then a higher percentage of the soybean seeds in the gibberellic acid solution Botany Abstracts will germinate than in the other solutions. 48 soybean seeds were germinated on cotton pads soaked in each solution for ten days. A similar percentage of seeds in the molybdenum, potassium chloride, gibberellic acid, and distilled water solutions germinated by the end of the duration of the experiment. Fewer seeds in the polyethylene glycol solution germinated, and the radicles of the germinated seeds in the polyethylene glycol solution were significantly shorter than the radicles of the seeds germinated in other solutions. The radicles of the seeds in the potassium chloride solution were longer than the radicles of the seeds in the other solutions. Cameron Phillips Household Plant Solutions BOT ECOL Gardeners everywhere try to find the best substances to help their plants grow taller and faster without polluting the water with chemicals. This experiment is about trying to find an environmentally safe liquid that will help the plants sprout the fastest and grow the tallest in the days provided. For our procedure we put two bean seeds in a cup of soil. We planted three cups for each liquid so we would have multiple trials. For the first two days we gave the plants 2 tablespoons of 75% water and 25% of each independent variable. From then on we used 2 tablespoons every other day.To control the experiment, we made sure all of our plants were in the same location. We wrote journal entries on the plants every day noting how the plants changed or did something odd. Our experiment showed that the plants with the tea grew an average height of 7.8 centimeters. The average for the controlled variable (just water) was 3.1 centimeters. The average height for the peroxide was 2.7 centimeters. The average for the lemon juice was 1 centimeter. The average for the salt water and the Mountain Dew was zero. Another thing we noticed is that Mountain Dew and lemon juice seemed to make the plants moldy which could be from the acid. Our conclusion is that tea helped the plants grow the fastest and the tallest. We think that the tea does well because it has natural ingredients. Luke Elliot Combs Household Plant Solutions BOT ecol Gardeners everywhere try to find the best substances to help their plants grow taller and faster without polluting the water with chemicals. This experiment is about trying to find an environmentally safe liquid that will help the plants sprout the fastest and grow the tallest in the days provided. For our procedure we put six seeds for each different liquid and put two in each cup. For the first two days we watered them. Then we watered them every other day.To control the experiment, we made sure all of our plants were in the same location. We wrote journal entries on the plants every day noting how the plants changed or did something odd. Our experiment showed that tea was the best for the plants because it grew the plants the fastest and the tallest. The peroxide seemed to help the plants just not as much as the tea did. Another thing we noticed is that Mountain Dew and lemon juice seemed to make the plants moldy which could be from the acid.
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