Experimental
Volume 25
Number 9
September 2016
Dermatology
The official Journal of
Arbeitsgemeinschaft
Dermatologische Forschung,
Editors:
Thomas A. Luger, Münster, Germany
Ralf Paus, Manchester, UK & Münster, Germany
European Immunodermatology Society,
Associate Editors: Tamás Biró, Debrecen, Hungary
Australasian Hair and Wool Research Society,
Markus Böhm, Münster, Germany
European Hidradenitis Suppurativa Foundation e.V.
Maksim Plikus, Irvine, CA, USA
Hidradenitis Suppurativa Foundation (USA), Inc. ADF Associate Editor: Karin Loser, Münster, Germany
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Instructions to authors
Presubmission inquiries should be sent to:
Editors:
Ralf Paus
Thomas A. Luger
Manchester, UK & Muenster, Germany
Department of Dermatology
Centre for Dermatology Research
University Hospital Münster
School of Biological Sciences
Von-Esmarch-Str. 58
University of Manchester
D-48149 Münster, Germany
Oxford Rd, Stopford Bldg, R.
Tel: +49-251-835-6504
3.050 Manchester M13 9PT, UK
Fax: +49-251-835-6522
E-mail: [email protected]
E-mail: [email protected]
Production Editor: [email protected]
Manuscript Submission: Experimental Dermatology is only accepting manuscripts electronically via an online
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Conflicts of Interest: When submitting a manuscript, authors are responsible for disclosing all financial and
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be included in the paper after the Acknowledgements section. In the event where there are no conflicts of
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The journal is a member of and subscribes to the principles of the Committee on Publication Ethics.
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Manuscripts describing studies involving animals should comply with local/national guidelines governing the
use of experimental animals and contain a statement indicating that the procedures have been approved by the
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Manuscript Arrangement
Whenever possible, authors are strongly encouraged to emphasize the clinical relevance of their work. In original
papers, at least the final paragraph of the paper should include a discussion of the potential clinical relevance of
the presented findings, whenever this makes sense.
Cover Letter
All submissions must be accompanied by a short cover letter, which contains the following information:
a) definition of the publication category for which the manuscript is being submitted
b) statement whether this is an unsolicited submission or has been invited by one of the editors/associate editors
or editorial board members (name)
c) statement that the applicable author guidelines for this publication category have been fully respected (or, if not,
justification why the manuscript should have e.g. an excess word count or number of figs./refs. etc. )
d) brief, but instructive synthesis that explains in which way the current manuscript advances the field (max. 150 words)
e) 5 key words that are most relevant to the contents of submitted manuscript and that are not contained in the
manuscript title (these are also to be stated on the title page of the manuscript itself)
f) recommendation of 3–4 recognized expert reviewers in the specific field(s) of research of the submitted manuscript
g) statement that none of the suggested referees has co-authored a publication during the past 4 years with any of
the co-authors of the submitted manuscript
Regular Articles: Research Articles may not exceed 4,000 words (not including tables, figures and references). They should
contain a maximum of 4 display items in single column width (including figures and tables). Clinical studies are explicitly
welcome, if they also contain major experimental research components. Please list the word count and number of display
items on the cover page of the manuscript. Manuscripts exceeding these limits will be returned to Authors without review.
Each manuscript component should begin on a new page in the following sequence: title page; abstract; key words (3 to
5); introduction; methods; results; discussion; acknowledgments; references; tables (each table, complete with title and
footnotes, on a separate page); legends for illustrations. Number pages consecutively, beginning with the title page. Type
the page number in the upper right-hand corner of each page. Number the tables consecutively with arabic numerals. For
any further details, consult the ‘Uniform requirements for manuscripts submitted to bio-medical journals’, Ref. (2) below.
Methods Reviews
Experimental Dermatology regularly publishes Methodological Reviews that provide state-of-the-art, authoritative coverage of
important laboratory techniques across all areas of investigative dermatology and molecular biomedicine. These critical reviews
also cover typical problems and limitations encountered with the discussed method, suggest problem solving approaches, and
offer pragmatic advice from experienced laboratory practitioners not only for newcomers but also readers interested in the
field. The submission of Methods Reviews, is invited by the Editors. If you are interested in writing a Methods Review, please
contact the Editors. METHODS REVIEW: max 3,000 words, plus 2 color plates, 1-2 tables and up to 100 refs.
Viewpoint, Review Articles:
Speculative, thought-provoking Viewpoint essays (no abstract, max. 2000 words, 9 references, resulting in max 3
publication pages) and standard review articles (max. 4000 words, 200 refs.) are invited by the editors or associate
editors. These always welcome specific suggestions for topics and prospective authors, ideally along with submission
of a brief structural outline. Instructions for the design of Viewpoints essays and Review articles are available from the
editorial office and details are negotiated with the editors.
Patterns of Expression: Patterns of Expression is a category of paper reporting the comprehensive pattern of expression
of a new or important gene during embryonic development and/or in the adult organism, highlighting interesting or
unexpected findings. Patterns of Expression papersshould contain data using multiple complimentary approaches,
including but not limited to, in situ hybridization, immunohistochemistry, immunoflourescence, real-time PCR, western
and northern blot. Papers should not exceed 2,500 words and do not require an invitation by the Editor. Illustration
should be stringently restricted to what is really essential in order to convincingly document the reported expression
pattern. Non-essential, Supporting Information, also in color, may be displayed free-of-charge on the journal website.
Letter to the Editor, Methods Letters to the Editor
Letters and Methods Letters to the Editors in Experimental Dermatology are to be organised as follows: 1000 words:
structured into BACKGROUND, QUESTIONS ADDRESSED, EXP. DESIGN, RESULTS, CONCLUSIONS; maximum 2 small figures or
tables and 9 references (unlimited number of supplementary references, to be cited as s1, s2, etc. in the Letter manuscript
and to be listed only in the supporting information); no abstract; resulting in max 2 publication pages; our letter format
also allows for essentially unlimited online supplementary data (including more extensive information on materials and
methods, more figures/tables and additional references). Please note that LETTERS and METHODS LETTERS TO THE EDITORS
must not exceed 2 online print pages including images/tables. As a special service to authors and readers, in contrast to
full original articles, LETTERS and METHODS LETTERS TO THE EDITORS in Exp Dermatol are made freely available to all readers
so as to render them maximally accessible (free access policy). The title of the Letter should be phrased as a statement
headline that transports a clear take home message, which is well-supported by the presented data.
Hypothesis Letter
HYPOTHESIS LETTERS offer a forum for the proposal of novel hypotheses on important skin research topics of general interest.
These hypotheses must be developed on the basis of the published literature, must be stated clearly and succinctly, and
must be experimentally testable. Hypotheses manuscripts that, if confirmed, are likely to lead to important changes in
dermatological therapy and/or pathobiology concepts are favored. Hypothesis Letters have a maximum of 900 words, 1
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composite figure, 9 references, no abstract, resulting in max 2 publication pages, and can be complemented by an extensive
supplement (with additional figs., text, and unlimited number of supplementary references, to be cited as s1, s2, etc. in the
Letter manuscript and to be listed only in the supporting information). They are to be structured as follows: Background,
Premises, Hypothesis, How to test the hypothesis, Relevance and perspectives. The title of the Letter should be phrased as
a statement headline that transports a clear take home message, which is well-supported by the presented data.
Letter: Mouse Mutants with Absent Skin Phenotype (MMASP)
This new publication category features genetic mouse manipulations in the skin (e.g. transgenic gene over/misexpression
or gene targeted ablation) that manifest a minimal or no detectable phenotype. Typically a considerable amount of
funding resources, research time and effort by students/postdocs has been invested to generate the necessary preliminary
data before abandoning a genetic mouse manipulation on project that yields no phenotype. In addition, several labs
may embark on similar genetic mouse manipulation efforts with ”negative” results, multiplying the loss of money/
time resources if the results are not reported. This new letter category will serve as a platform to share such findings
by reporting high-quality data that document successful gene manipulation at the RNA/protein level with several
complementary methods, such as in situ hybridization, immunofluorescence/histochemistry, genotyping and real-time
PCR of isolated cells, and an in-depth ”absence of phenotype” analysis of skin tissues at relevant time points (including
rigorous quantifications) using tissue stains and cell-type specific gene marker analysis. Letters should be less than 1000
words with a maximum of 2 figures and 9 references , no abstract, resulting in max 2 publication pages. Additional figures,
materials, and methods are allowed in a generous ”Supporting Information” section. The title of the Letter should be
phrased as a statement headline that transports a clear take home message, which is well-supported by the presented data.
Bedside-to-bench Letter: This novel type of LETTER is intended to close a long-existing gap between classical case
reports, hypothesis letters and viewpoints. To date, case reports are only considered in Experimental Dermatology
if they contain a substantial experimental data component. Instead, "BEDSIDE-TO-BENCH LETTERS" can now be used
as a unique platform for bridging clinical and experimental dermatology by calling attention to intriguing and
instructive clinical cases that shed light on disease pathobiology or novel skin biology aspects.
The key characteristic for a case report manuscript to be eligible for publication in Experimental Dermatology as
a "BEDSIDE-TO-BENCH LETTER" is that the authors can develop a clear-cut strategy for experimental research that
emanates from the case they are reporting and that promises important advances in translational skin biology or
skin pathology. For example, a clinician observing an unexpected improvement of a skin disease by a novel biologic
or targeted treatment that was initiated to treat another disease of the patient, or who observes an interesting skin
sign or symptom that raises fundamental questions on basic skin biology or pathology which can be addressed in a
well-defined set of experiments, is strongly invited to transform this observation into a "BEDSIDE-TO-BENCH LETTER".
The structure of this innovative type of LETTER manuscript must include (1) a concise presentation of the case supported
by key photographic documentation ideally together with histology or immunohistology ( = bedside aspect), (2)
proposal of the obtained novel insight into disease pathogenesis, skin biology, or new treatment strategy, and (3)
detailed outline of a translational research program (= bench). The maximum length of this "BEDSIDE-TO-BENCH LETTER"
is 800 words with 1 composite figure and 9 references no abstract, resulting in max 2 publication pages. Authors are
encouraged to make use of an extensive e-supplement with additional text, figures, tables and references.
Like all LETTERS in Experimental Dermatology this new LETTER category will be freely accessible via PubMed without the
need to have a subscription to the journal and thus enjoys unusually good public visibility. The title of the Letter should be
phrased as a statement headline that transports a clear take home message, which is well-supported by the presented data.
Commentary
-Comments on papers (incl. Letters) published in Experimental Dermatology during the past 4 months;
Commentaries are typically invited, but unsolicited commentaries are welcome
-labeled as COMMENTARY or COMMENTARY FROM THE EDITORIAL BOARD (where applicable)
-cite the study you comment on within the text (Exp Derm abbreviated citation style)
-word limit: 800-1000 words, inclusion of a figure/cartoon and/or table is welcome
-the max. number of refs. is 9 (unlimited number of supplementary references, to be cited as s1, s2, etc.
in the Commentary manuscript and to be listed only in the supporting information)
-no abstract
-max. online print number of pages including images/tables/references: 2
Commentary: My favorite historical paper
This new publication category features short commentaries that call attention to a seminal historical paper (i.e. original
article, review, letter, or book chapter), which currently is not widely known or cited any longer and which the author
feels strongly should be brought back to the attention of a wide range of readers and disciplines. Title: needs to attract
attention of both experts and non-cognoscenti; must indicate the relevant field of skin research, and should encapsulate
at least a first hint at why this paper is important. Text: 600-1000 words, up to 9 references, 1 figure or table and
unlimited supplement no abstract, resulting in max 2 publication pages, The commentary should argue succinctly why
the author feels that the selected historical paper is fascinating; has made a major contribution to its field of research and/
or is very important for other reasons of general interest. Please elaborate also how this paper has provided major novel
concepts or very important pointers that remain pertinent today and are relevant for future research in this area. Ideally,
the author should obtain permission from the relevant publisher to reprint the entire historical article in the supplement
of the commentary so that the full historical paper becomes easily and generally accessible.
We explicitly recommend to extensively complement all of the above publication features with an elaborate supplement
(as much supplementary text and as many supplementary figures, tables & references as are considered useful;
supplementary text blocks should be referred to in the core manuscript as T1, T2, ...; supplementary figures as S1, S2,
...; supplementary tables as ST1, ST2, ...; supplementary references as s1, s2, ... [full citations only in the supplement]
Illustrations: All figures should clarify the text and their number should be kept to a minimum. Details must be large
enough to retain their clarity after reduction. Illustrations should preferably fill single column width (81 mm) after
reduction, although in exceptional cases 2/3 width (120 mm) or full page width (168 mm) will be accepted. Line drawings
should be professionally drafted and photographed; halftones should exhibit high contrast. A linear size scale should be
incorporated into electron microscopic pictures. Colour illustrations will be accepted free of charge.
Abbreviations and Symbols: Use only standard abbreviations. All units will be metric. Avoid roman numerals in the text. In decimals, a decimal point, and not a comma, will be used. Consult the CBE style manual:
a guide for authors, editors and publishers in the biological sciences, 4th edition, Arlington, Virginia, 1978.
Avoid abbreviations in the title. The full term for which an abbreviation stands should precede its first use
in the text unless it is a standard unit of measurement. Units of measurement should be expressed in accordance
with the SI and CIE recommendations (see Ref. 3, below).
References: AMA – American Medical Association.
All references should be numbered consecutively in order of appearance and should be as complete as
possible. In text citations should cite references in consecutive order using Arabic superscript numerals.
Sample references follow:
Journal article: 1. King VM, Armstrong DM, Apps R, Trott JR. Numerical aspects of pontine, lateral reticular,
and inferior olivary projections to two paravermal cortical zones of the cat cerebellum. J Comp Neurol
1998;390:537-551.
Book: 2. Voet D, Voet JG. Biochemistry. New York: John Wiley & Sons; 1990. 1223 p. Please note that journal
title abbreviations should conform to the practices of Chemical Abstracts.
For more information about AMA reference style - AMA Manual of Style.
Pre-submission English-language editing: Authors for whom English is a second language may choose to have
their manuscript professionally edited before submission or during the review process to improve the English.
A list of independent suppliers of editing services can be found at http:// authorservices.wiley.com/bauthor/
english_language.asp. Japanese authors can also find a list of local English improvement services at
http://www.wiley.co.jp/journals/editcontribute.html. All services are paid for and arranged by the author, and
use of one of these services does not guarantee acceptance or preference for publication.
Proofs: The corresponding author will receive an email alert containing a link to a web site. A working e-mail address
must therefore be provided for the corresponding author. The proof can be downloaded as a PDF (portable document
format) file from this site. Acrobat Reader will be required in order to read this file. This software can be downloaded
(free of charge) from the following web site: http://www.adobe.com/products/acrobat/readstep2.html.
This will enable the file to be opened, read on screen and e-annotated or printed out in order for any corrections
to be added. Further instructions will be sent with the proof. Excessive changes made by the author in the proofs,
excluding typesetting errors, will be charged separately.
Offprints: Authors will be provided with electronic offprints of their paper. Paper offprints may be ordered online and further
details accompany proofs. Offprints are normally dispatched within three weeks of publication of the issue in which the
paper appears. Please contact the following if offprints do not arrive: C.O.S. Printers Pte Ltd., 9 Kian Teck Crescent, Singapore
628875; Fax: +65 6265 9074; Email: offprints@cosprinters com. However, please note that offprints are sent by surface mail,
so overseas orders may take up to six weeks to arrive. Electronic offprints are sent to the first author at his or her first email
address on the title page of the paper, unless advised otherwise; therefore please ensure that the name, address and email
of the receiving author are clearly indicated on the manuscript title page if he or she is not the first author of the paper.
Author material archive policy: Please note that unless specifically requested, Wiley will dispose of all hardcopy
or electronic material submitted 2months after publication. If you require the return of any material submitted,
please inform the editorial office or production editor as soon as possible if you have not yet done so.
Online Open: Experimental Dermatology accepts articles for Open Access publication. Please visit http://
olabout.wiley.com/WileyCDA/Section/id-406241.html for further information about OnlineOpen.
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Experimental
Dermatology
Contents: Volume 25 • Number 9 • September 2016
Methods Review
A primer for studying cell cycle dynamics of the human hair follicle
Viewpoint
Any route for melanoblasts to colonize the skin!
Commentaries
The role of Syk in cutaneous lupus erythematosus
Pro-inflammatory sebocyte growth and survival signalling in acne vulgaris are
reversed by pro-apoptotic isotretinoin signalling
Original Articles
Topical betamethasone butyrate propionate exacerbates pressure ulcers after
cutaneous ischemia–reperfusion injury
Validation of a diagnostic algorithm for the discrimination of actinic keratosis from
normal skin and squamous cell carcinoma by means of high-definition optical
coherence tomography
Comparison of the acute ultraviolet photoresponse in congenic albino hairless
C57BL/6J mice relative to outbred SKH1 hairless mice
Activation of the human keratinocyte B1 bradykinin receptor induces expression and
secretion of metalloproteases 2 and 9 by transactivation of epidermal growth factor
receptor
Differential effectiveness of selected non-psychotropic phytocannabinoids on human
sebocyte functions implicates their introduction in dry/seborrhoeic skin and acne
treatment
Distinctive molecular responses to ultraviolet radiation between keratinocytes and
melanocytes
The differentiation of amniotic fluid stem cells into sweat glandlike cells is enhanced
by the presence of Sonic hedgehog in the conditioned medium
Bedside-to-Bench-Letter
Absence or low density of Propionibacterium acnes in comedonal lesions of acne
patients? A surface to inside study of skin fluorescence
Methods Letter to the Editor
Optical coherence tomography angiography for longitudinal monitoring of vascular
changes in human cutaneous burns
Letter: Mouse Mutants with Absent or Minimal Skin Phenotype
Galanin 3 receptor-deficient mice show no alteration in the oxazolone-induced
contact dermatitis phenotype
Letters to the Editor
Quantitative changes of melanoma-associated antigens as a biomarker for targeted
therapy response
Metabolomic analysis of amino acids and lipids in human hair altered by dyeing,
perming and bleaching
Endoplasmic reticulum stress is associated with the pathogenesis of pemphigus
vulgaris
Tumor suppressor identity can contribute to heterogeneity of phenotype in hair
follicle stem cell-induced squamous cell carcinoma
Long-term skin regeneration in xenografts from iPSC teratoma-derived human
keratinocytes
Phycosaccharide AI, a mixture of alginate polysaccharides, increases stem cell
proliferation in aged keratinocytes
UVB irradiation induces HMGB1 expression in keratinocytes without promoting
apoptosis
663
T. S. Purba, L. Brunken, N. J. Hawkshaw, M. Peake,
J. Hardman & R. Paus
669
V. Petit & L. Larue
674
676
G.-M. Deng & G. C. Tsokos
B. C. Melnik
678
A. Uchiyama, K. Yamada, B. Perera, S. Ogino,
Y. Yokoyama, Y. Takeuchi, O. Ishikawa & S.-I. Motegi
A. Marneffe, M. Suppa, M. Miyamoto, V. Del Marmol &
M. Boone
684
688
694
R. L. Konger, E. Derr-Yellin, D. Hojati, C. Lutz &
J. P. Sundberg
C. E. Matus, P. Ehrenfeld, F. Pavicic, C. B. González,
M. Concha, K. D. Bhoola, R. A. Burgos & C. D. Figueroa
701
A. Oláh, A. Markovics, J. Szabó-Papp, P. T. Szabó,
C. Stott, C. C. Zouboulis & T. Bíró
708
X. Sun, A. Kim, M. Nakatani, Y. Shen & L. Liu
714
H. Liang, Q. Sun, Y. Zhen, F. Li, Y. Xu, Y. Liu, X. Zhang &
M. Qin
721
D.-T. Xu, X.-L. Qi, Y. Cui & W. Liu
723
P. Gong, S. Es'haghian, F. M. Wood, D. D. Sampson &
R. A. McLaughlin
726
B. Botz, S. M. Brunner, Á. Kemény, E. Pintér,
J. J. McDougall, B. Kofler & Z. Helyes
729
739
P. Arenberger, A. Fialova, S. Gkalpakiotis, A. Pavlikova &
M. Arenbergerova
K.-M. Joo, A.-R. Kim, S.-N. Kim, B.-M. Kim, H. K. Lee,
S. Bae, J.-H. Lee & K.-M. Lim
C. Mihailidou, N. Katsoulas, E. Panagiotou, E. Farmaki,
A. Sklavounou, H. Kiaris & I. Chatzistamou
A. Flores, W. Grant, A. C. White, P. Scumpia, R. Takahashi
& W. E. Lowry
M. García, O. Quintana-Bustamante, J. C. Segovia,
J. Bueren, L. Martinez-Santamaría, S. Guerrero-Aspizua,
M. J. Escamez, M. DelRio & F. Larcher
A. Charruyer, S. Fong, L. Yue, S. T. Arron & R. Ghadially
742
K. Torii & A. Morita
731
733
735
737
Printed in the USA by The Sheridan Group
ISSN 0906-6705
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Experimental Dermatology
Vol. 25 No. 9 September 2016 pages 661–744
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