Taxonomic description and Genome sequence of Rheinheimera mesophila sp. nov.,
isolated from a industrial waste site
Anand Kumar., Abhay Bajaj, Rajendran Mathan Kumar., Gurwinder Kaur., Navjot
Kaur., Nitin Kumar Singh, Natesan Manickam and Shanmugam Mayilraj
Journal Name: International Journal of Systematic and Evolutionary Microbiology
Affiliation: MTCC- Microbial Type Culture Collection & Gene Bank, CSIR- Institute of
Microbial Technology, Chandigarh, 160036, India and Environmental Biotechnology
Division, CSIR-Indian Institute of Toxicological Research (IITR), Lucknow, 226001, India.
Correspondence: Shanmugam Mayilraj; [email protected]
Natesan Manickam, [email protected]
Supplementary Fig. S1. Virtual RFLP patterns derived from the in silico digestions of the
16S rRNA gene of strain IITR-13T: lane 1. EcoRII; 2, BfaI; 3, BstUI; 4, HaeIII; 5, HhaI; 6,
HinfI; 7, Kpn2I; 8, MseI; 9, SmaI; 10, TaaI; 11, Hi Lo DNA marker; 12, Sau3AI; 13, AluI; 14,
MspI; 15, RsaI, 16, MboI; 17, BmiI; 18, AhlI; 19, SatI; 20, GlaI; 21, EcoNI; 22, ZraI; 23,
EcoRI; 24, StuI and 25, XapI. MW, Hi Lo DNA marker.
Supplementary Fig. S2. Virtual RFLP patterns derived from the in silico digestions of the
16S rRNA gene of strain Rheinheimera tangshanensis JA3-B52T: lane 1. EcoRII; 2, BfaI; 3,
BstUI; 4, HaeIII; 5, HhaI; 6, HinfI; 7, Kpn2I; 8, MseI; 9, SmaI; 10, TaaI; 11, Hi Lo DNA
marker; 12, Sau3AI; 13, AluI; 14, MspI; 15, RsaI, 16, MboI; 17, BmiI; 18, AhlI; 19, SatI; 20,
GlaI; 21, EcoNI; 22, ZraI; 23, EcoRI; 24, StuI and 25, XapI. MW, Hi Lo DNA marker.
Supplementary Fig. S3. Virtual RFLP patterns derived from the in silico digestions of the
16S rRNA gene of strain Rheinheimera texasensis A62-14BT: lane 1. EcoRII; 2, BfaI; 3,
BstUI; 4, HaeIII; 5, HhaI; 6, HinfI; 7, Kpn2I; 8, MseI; 9, SmaI; 10, TaaI; 11, Hi Lo DNA
marker; 12, Sau3AI; 13, AluI; 14, MspI; 15, RsaI, 16, MboI; 17, BmiI; 18, AhlI; 19, SatI; 20,
GlaI; 21, EcoNI; 22, ZraI; 23, EcoRI; 24, StuI and 25, XapI. MW, Hi Lo DNA marker.
Supplementary Fig. S4. Virtual RFLP patterns derived from the in silico digestions of the
16S rRNA gene of strain Rheinheimera soli BD-d46T: lane 1. EcoRII; 2, BfaI; 3, BstUI; 4,
HaeIII; 5, HhaI; 6, HinfI; 7, Kpn2I; 8, MseI; 9, SmaI; 10, TaaI; 11, Hi Lo DNA marker; 12,
Sau3AI; 13, AluI; 14, MspI; 15, RsaI, 16, MboI; 17, BmiI; 18, AhlI; 19, SatI; 20, GlaI; 21,
EcoNI; 22, ZraI; 23, EcoRI; 24, StuI and 25, XapI. MW, Hi Lo DNA marker.
(a)
(c)
(b)
(d)
Supplementary Fig. S5. Two dimensional TLC of polar lipids of strain (a) IITR-13T, (b) R.
texasensis DSM 17496T, (c) R. tangshanensis DSM 19460T, and (d) R. soli DSM 19413T;
PG, phosphatidylglycerol; PE. phosphotidylethnalamine; PI, phosphatidylinositol; PL,
unknown phospholipids. Solvent system for 1st run; chloroform (190ml): methanol (75ml):
water (12ml). Second run; chloroform (200ml): acetic acid (37.5ml): methanol (30ml): water
(10ml) were used.
Supplementary Fig. S6. Sub-system distribution of strain IITR-13T based on RAST
annotation.
5267.504 m/z
Supplementary Fig. S7. The gel view displays a pseudo gel representation of spectral
fingerprint. It also represents a sort of heat map in which red color equals to not related and
blue equals to closely related. Strain(s) 1. R. texasensis (DSM 17496T), 4. R. tangshanensis
(DSM 19460T), 5. IITR-13T.
Supplementary table S1. Summary of the annotated genome of strain IITR-13T
Characters
Length (bp)
N75
55,699
N50
1,03,330
Largest contig
2,90,931
Contigs
102
G+C mol%
47.8
Total
3,74,9903