Electronic Supplementary Material (ESI) for Analytical Methods.
This journal is © The Royal Society of Chemistry 2016
Total content determination for the effective fraction of the
alkaloids in Dicranostigma leptopodum (Maxim.) Fedde by high
performance liquid chromatography and ultraviolet-visible
spectrophotometry
Yali Chena,b, Ruxia Lia, Rongrong Gaoa, Qian Yana, Mei Zhonga, Junxi Liua,*, Quanyi Zhaob, Duolong Dia
a
Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,
Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China
b
Institute of Medicinal Chemistry, School of Pharmacy, Lanzhou University, Lanzhou 730000, PR China
* Corresponding author. Tel.: +86 931 4968212; Fax.: +86 931 8277088.
E-mail address: [email protected] (J.X. Liu)
Table S1. The physical parameters of MARs
MARs
Moisture content(%)
Particle size(mm)
specific surface
area(m2/g)
AB-8
50.48
0.3-1.25
450-500
D101
60.75
0.25-0.84
480-520
LK-001
50.58
0.3-1.25
--
LX-17
52.70
0.3-1.25
--
LX-28
60.54
0.3-1.25
460-590
Table S2. The symmetry of target alkaloids detected on SinoChrom ODS-BP column
and Megres C18 column.
5hydroxy
coptisine
Isocorytu
bertine
Protopine
Allocrypt
opine
Coptisine
Berberine
Corydine
Isocorydi
ne
ODS-BPa
0.48
1.12
0.60
0.79
0.62
0.44
0.94
0.90
ODS-BPb
0.52
0.97
0.91
0.64
0.97
0.99
Megresa
1.09
1.32
1.04
0.85
0.74
0.75
0.93
0.90
Megresb
0.85
0.91
0.86
0.99
0.76
0.91
0.98
1.02
0.74
a
with no addition of ionic liquid in mobile phase.
b
with addition of ionic liquid in mobile phase.
Fig S1. Chromatogram of blank solvent determined on Megres C18 column.
Conditions: Megres C18 column; Mobile phase: acetonitrile (A), 0.2% phosphoric
acid and 0.4% (w/v) BmimBF4 in water adjusted to pH 6.3 by the addition of
triethylamine solution (B); gradient: 20% (A) at 0–20 min, 20-45% (A) at 20-35min;
injection volume: 20 μL; flow rate: 1.0 mL/min.