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BG-Maleimide
Catalog #
S9153S
Size
2 mg
Concentration
Categories: Building Blocks, Protein Labeling, SNAP-tag Substrates
Applications: Cell Imaging, Protein Labeling (SNAP/CLIP)
Product
Information
FAQs &
Tech Tips
Protocols &
Manuals
Other Tools &
Resources
Description
References
Quality &
Safety
Legal
Information
Notes
Description
BG-Maleimide is a thiol-reactive building block for the one-step synthesis of SNAP-tag® substrates from thiol-containing precursors such as
thiol-modified oligonucleotides. This building block allows you to make custom SNAP-tag substrates for labeling SNAP-tag fusion proteins
for a wide range of applications such as the coupling of proteins and solid surfaces such as DNA chips (1).
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The
SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or
beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of
the substrate is covalently attached to the SNAP-tag.
There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the
fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with
SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described in this document.
Figure 1: Structure of BG-Maleimide (MW 489.5 g/mol)
Notes
1. Dry BG-Maleimide should be stored at -20°C.
2. The BG-Maleimide building block should be dissolved in anhydrous DMF. Choose a solvent volume that will be compatible with the final
use. Unused building block dissolved in DMF should be stored at -20°C.
References
1. Jongsma, M.A. and Litjens, R.H. (2006). Proteomics. 6, 2650-2655.
FAQs
Tech Tips
FAQs
1. Cellular Imaging and Analysis FAQs
Tech Tips
test
Protocols
Datacards
Protocols
1. Reaction Conditions for Chemical Coupling (S9153)
2. Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9153)
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
S9153Datasheet-Lot0041203
S9153Datasheet-Lot0051207
S9153Datasheet-Lot0071501
Selection Charts
Selection Charts
Building Blocks
Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
Troubleshooting Guides
Labeling with SNAP-tag® Technology Troubleshooting Guide
Troubleshooting Guides
Quality Control
Datacards
Safety Data Sheet
Quality Control
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product.
Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting
Documents section of this page. Further information regarding NEB product quality can be found here.
Physical Purity (HPLC):
The purity of the product is determined by HPLC analysis.
Safety Data Sheet
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
BG-Maleimide
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
S9153Datasheet-Lot0041203
S9153Datasheet-Lot0051207
S9153Datasheet-Lot0071501
Legal and Disclaimers
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc
(NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain
additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes
in humans or animals.
Licenses
Notice To Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH
AND DEVELOPMENT PURPOSES ONLY. Commercial use of this system or any components thereof requires a license from
New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938.
Cellular Imaging and Analysis (i.e., SNAP and CLIP products)
Notice to Buyer/User: The Buyer/User has a non-exclusive license to use this system or any component thereof for RESEARCH
AND DEVELOPMENT ONLY. Commercial use of this system or any components thereof requires a license from New England
Biolabs, Inc., 240 County Road, Ipswich, MA 01938.
These patents and patent applications are owned by Covalys, or owned by the Ecole Polytechnique Fédérale de Lausanne (EPFL)
and exclusively licensed to Covalys and NEB.
The products and/or their use may be covered by one or more of the following patents and patent applications:
Methods for Using O6-Alkylguanine-DNA-Alkyltransferases: US 7,939,284; US 8,367,361; EP 1 410 023; EP 2 037 271; EP 1 696
234; EP 2 211 177; JP 4195815; JP 4226053; CN 1295510; CN 1975422; and SG 100125.
Substrates for O6-Alkylguanine-DNA Alkyltransferase: US 7,799,524; and JP 4976651.
Mutants of O6-Alkylguanine-DNA-Alkyltransferases: US 7,888,090. EP 1720981 (pending).
Specific Substrates for O6-Alkylguanine-DNA-Alkyltransferases: US 8,163,479. EP 1730298 (pending).