EORGIAN
EDICAL
EWS
ISSN 1512-0112
No 4 (205) Апрель 2012
ТБИЛИСИ - NEW YORK
ЕЖЕМЕСЯЧНЫЙ НАУЧНЫЙ ЖУРНАЛ
Медицинские новости Грузии
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GEORGIAN
MEDICAL
NEWS
No 4 (205) 2012
Published in cooperation with and under the patronage
of the Tbilisi State Medical University
Издается в сотрудничестве и под патронажем
Тбилисского государственного медицинского университета
gamoicema Tbilisis saxelmwifo samedicino universitetTan
TanamSromlobiTa da misi patrona;iT
ЕЖЕМЕСЯЧНЫЙ НАУЧНЫЙ ЖУРНАЛ
ТБИЛИСИ - НЬЮ-ЙОРК
GMN: Georgian Medical News is peer-reviewed, published monthly journal committed to promoting
the science and art of medicine and the betterment of public health, published by the GMN Editorial
Board and The International Academy of Sciences, Education, Industry and Arts (U.S.A.) since
1994. GMN carries original scientific articles on medicine, biology and pharmacy, which are of
experimental, theoretical and practical character; publishes original research, reviews, commentaries,
editorials, essays, medical news, and correspondence in English and Russian.
GMN is indexed in MEDLINE, SCOPUS, VINITI Russian Academy of Sciences. The full text
content is available through EBSCO databases.
GMN: Медицинские новости Грузии - ежемесячный рецензируе­мый научный журнал,
издаётся Редакционной коллегией и Международной академией наук, образования, искусств и
естествознания (IASEIA) США с 1994 года на русском и английском языках в целях поддержки
медицинской науки и улучшения здравоохранения. В журнале публикуются оригинальные
научные статьи в области медицины, биологии и фармации, статьи обзорного характера,
рецензии, научные сообщения, новости медицины и здравоохранения.
Журнал индексируется в MEDLINE, отражён в базе данных SCOPUS и ВИНИТИ РАН.
Полнотекстовые статьи журнала доступны через БД EBSCO.
GMN: Georgian Medical News – saqarTvelos samedicino siaxleni – aris yovelTviuri
samecniero samedicino recenzirebadi Jurnali, gamoicema 1994 wlidan, warmoadgens
saredaqcio kolegiisa da aSS-is mecnierebis, ganaTlebis, industriis, xelovnebisa
da bunebismetyvelebis saerTaSoriso akademiis erTobliv gamocemas. GMN-Si rusul,
inglisur da germanul enebze qveyndeba eqsperimentuli, Teoriuli da praqtikuli
xasiaTis originaluri samecniero statiebi medicinis, biologiisa da farmaciis
sferoSi, mimoxilviTi xasiaTis statiebi, recenziebi.
Jurnali indeqsirebulia MEDLINE-is saerTaSoriso sistemaSi, asaxulia SCOPUSis da ВИНИТИ РАН-is monacemTa bazebSi. statiebis sruli teqsti xelmisawvdomia
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МЕДИЦИНСКИЕ НОВОСТИ ГРУЗИИ
Ежемесячный совместный грузино-американский научный электронно-печатный журнал
Агентства медицинской информации Ассоциации деловой прессы Грузии,
Академии медицинских наук Грузии, Международной академии наук, индустрии,
образования и искусств США.
Издается с 1994 г., распространяется в СНГ, ЕС и США
НАУЧНЫЙ РЕДАКТОР
Лаури Манагадзе
ГЛАВНЫЙ РЕДАКТОР
Нино Микаберидзе
НАУЧНО-РЕДАКЦИОННЫЙ СОВЕТ
Лаури Манагадзе - председатель Научно-редакционного совета
Архимандрит Адам - Вахтанг Ахаладзе, Амиран Антадзе, Нелли Антелава,
Лео Бокерия, Отар Герзмава, Лиана Гогиашвили, Николай Гонгадзе,
Ирина Квачадзе, Нана Квирквелия, Зураб Кеванишвили, Палико Кинтраиа, Теймураз Лежава,
Джанлуиджи Мелотти, Караман Пагава, Николай Пирцхалаишвили, Мамука Пирцхалаишвили,
Вадим Саакадзе, Вальтер Стакл, Фридон Тодуа, Кеннет Уолкер, Рамаз Хецуриани,
Рудольф Хохенфеллнер, Рамаз Шенгелия
НАУЧНО-РЕДАКЦИОННАЯ КОЛЛЕГИЯ
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Михаил Бахмутский (США), Александр Геннинг (Германия),
Амиран Гамкрелидзе (Грузия), Константин Кипиани (Грузия),
Георгий Кавтарадзе (Грузия), Георгий Камкамидзе (Грузия),
Паата Куртанидзе (Грузия),Вахтанг Масхулия (Грузия),
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GEORGIAN MEDICAL NEWS
Monthly Georgia-US joint scientific journal published both in electronic and paper
formats of the Agency of Medical Information of the Georgian Association of Business
Press; Georgian Academy of Medical Sciences; International Academy of Sciences,
Education, Industry and Arts (USA).
Published since 1994. Distributed in NIS, EU and USA.
SCIENTIFIC EDITOR
Lauri Managadze
EDITOR IN CHIEF
Nino Mikaberidze
SCIENTIFIC EDITORIAL COUNCIL
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Archimandrite Adam - Vakhtang Akhaladze, Amiran Antadze, Nelly Antelava,
Leo Bokeria, Otar Gerzmava, Liana Gogiashvili, Nicholas Gongadze, Rudolf
Hohenfellner, Zurab Kevanishvili, Ramaz Khetsuriani, Paliko Kintraia,
Irina Kvachadze, Nana Kvirkvelia, Teymuraz Lezhava, Gianluigi Melotti,
Kharaman Pagava, Nicholas Pirtskhalaishvili, Mamuka Pirtskhalaishvili,
Vadim Saakadze, Ramaz Shengelia, Walter Stackl, Pridon Todua, Kenneth Walker
SCIENTIFIC EDITORIAL BOARD
Zurab Vadachkoria - Head of Editorial board
Michael Bakhmutsky (USA), Alexander Gënning (Germany),
Amiran Gamkrelidze (Georgia), David Elua (USA), Konstantin Kipiani (Georgia),
Giorgi Kavtaradze (Georgia), Giorgi Kamkamidze (Georgia),
Paata Kurtanidze (Georgia),Vakhtang Maskhulia (Georgia),
Tamara Mikaberidze (Georgia),Tengiz Riznis (USA)
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использовать AcadNusx. Размер шрифта - 12. К рукописи, напечатанной на компьютере, должен
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включая указатель литературы и резюме на английском, русском и грузинском языках.
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журналы», принятых Международным комитетом редакторов медицинских журналов http://www.spinesurgery.ru/files/publish.pdf и http://www.nlm.nih.gov/bsd/uniform_requirements.html
В конце каждой оригинальной статьи приводится библиографический список. В список литературы включаются все материалы, на которые имеются ссылки в тексте. Список составляется
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русском и грузинском языках (включающее следующие разделы: вступление, материал и методы,
результаты и заключение) и список ключевых слов (key words).
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REQUIREMENTS
Please note, materials submitted to the Editorial Office Staff are supposed to meet the following requirements:
1. Articles must be provided with a double copy, in English or Russian languages and typed or computer-printed on a single side of standard typing paper, with the left margin of 3 centimeters width, and 1.5 spacing
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materials). With computer-printed texts please enclose a CD carrying the same file titled with Latin symbols.
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3. Submitted material must include a coverage of a topical subject, research methods, results,
and review.
Authors of the scientific-research works must indicate the number of experimental biological species drawn in, list the employed methods of anesthetization and soporific means used during acute tests.
4. Tables must be presented in an original typed or computer-printed form, instead of a photocopied
version. Numbers, totals, percentile data on the tables must coincide with those in the texts of the
articles. Tables and graphs must be headed.
5. Photographs are required to be contrasted and must be submitted with doubles. Please number
each photograph with a pencil on its back, indicate author’s name, title of the article (short version), and
mark out its top and bottom parts. Drawings must be accurate, drafts and diagrams drawn in Indian ink (or
black ink). Photocopies of the X-ray photographs must be presented in a positive image in tiff format.
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the subtitles for the microphotographs please indicate the ocular and objective lens magnification power,
method of coloring or impregnation of the microscopic sections (preparations).
6. Please indicate last names, first and middle initials of the native authors, present names and initials
of the foreign authors in the transcription of the original language, enclose in parenthesis corresponding
number under which the author is listed in the reference materials.
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Editors guidance in its Uniform Requirements for Manuscripts Submitted to Biomedical Journals publication available online at: http://www.nlm.nih.gov/bsd/uniform_requirements.html
http://www.icmje.org/urm_full.pdf
In GMN style for each work cited in the text, a bibliographic reference is given, and this is located at the end
of the article under the title “References”. All references cited in the text must be listed. The list of references should be arranged alphabetically and then numbered. References are numbered in the text [numbers
in square brackets] and in the reference list and numbers are repeated throughout the text as needed. The
bibliographic description is given in the language of publication (citations in Georgian script are followed
by Cyrillic and Latin).
8. To obtain the rights of publication articles must be accompanied by a visa from the project instructor or the establishment, where the work has been performed, and a reference letter, both written or
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original text.
12. Sending in the works that have already been assigned to the press by other Editorial Staffs or
have been printed by other publishers is not permissible.
Articles that Fail to Meet the Aforementioned
Requirements are not Assigned to be Reviewed.
avtorTa sayuradRebod!
redaqciaSi statiis warmodgenisas saWiroa davicvaT Semdegi wesebi:
1. statia unda warmoadginoT 2 calad, rusul an inglisur enebze, dabeWdili
standartuli furclis 1 gverdze, 3 sm siganis marcxena velisa da striqonebs
Soris 1,5 intervalis dacviT. gamoyenebuli kompiuteruli Srifti rusul da inglisurenovan teqstebSi - Times New Roman (Кириллица), xolo qarTulenovan teqstSi
saWiroa gamoviyenoT AcadNusx. Sriftis zoma – 12. statias Tan unda axldes CD
statiiT.
2. statiis moculoba ar unda Seadgendes 6 gverdze naklebsa da 15 gverdze mets
literaturis siis da reziumeebis (inglisur, rusul da qarTul enebze) CaTvliT.
3. statiaSi saWiroa gaSuqdes: sakiTxis aqtualoba; kvlevis mizani; sakvlevi
masala da gamoyenebuli meTodebi; miRebuli Sedegebi da maTi gansja. eqsperimentuli xasiaTis statiebis warmodgenisas avtorebma unda miuTiTon saeqsperimento
cxovelebis saxeoba da raodenoba; gautkivarebisa da daZinebis meTodebi (mwvave
cdebis pirobebSi).
4. cxrilebi saWiroa warmoadginoT nabeWdi saxiT. yvela cifruli, Semajamebeli da procentuli monacemebi unda Seesabamebodes teqstSi moyvanils.
5. fotosuraTebi unda iyos kontrastuli; suraTebi, naxazebi, diagramebi
- dasaTaurebuli, danomrili da saTanado adgilas Casmuli. rentgenogramebis
fotoaslebi warmoadgineT pozitiuri gamosaxulebiT tiff formatSi. mikrofotosuraTebis warwerebSi saWiroa miuTiToT okularis an obieqtivis saSualebiT
gadidebis xarisxi, anaTalebis SeRebvis an impregnaciis meTodi da aRniSnoT suraTis zeda da qveda nawilebi.
6. samamulo avtorebis gvarebi statiaSi aRiniSneba inicialebis TandarTviT,
ucxourisa – ucxouri transkri pciiT.
7. statias Tan unda axldes avtoris mier gamoyenebuli samamulo da ucxouri Sromebis bibliografiuli sia (bolo 5-8 wlis siRrmiT). anbanuri wyobiT
warmodgenil bibliografiul siaSi miuTiTeT jer samamulo, Semdeg ucxoeli
avtorebi (gvari, inicialebi, statiis saTauri, Jurnalis dasaxeleba, gamocemis
adgili, weli, Jurnalis #, pirveli da bolo gverdebi). monografiis SemTxvevaSi
miuTiTeT gamocemis weli, adgili da gverdebis saerTo raodenoba. teqstSi
kvadratul fCxilebSi unda miuTiToT avtoris Sesabamisi N literaturis siis
mixedviT.
8. statias Tan unda axldes: a) dawesebulebis an samecniero xelmZRvanelis wardgineba, damowmebuli xelmoweriTa da beWdiT; b) dargis specialistis
damowmebuli recenzia, romelSic miTiTebuli iqneba sakiTxis aqtualoba, masalis
sakmaoba, meTodis sandooba, Sedegebis samecniero-praqtikuli mniSvneloba.
9. statiis bolos saWiroa yvela avtoris xelmowera, romelTa raodenoba
ar unda aRematebodes 5-s.
10. statias Tan unda axldes reziume inglisur, rusul da qarTul enebze
aranakleb naxevari gverdis moculobisa (saTauris, avtorebis, dawesebulebis miTiTebiT da unda Seicavdes Semdeg ganyofilebebs: Sesavali, masala da meTodebi,
Sedegebi da daskvnebi; teqstualuri nawili ar unda iyos 15 striqonze naklebi)
da sakvanZo sityvebis CamonaTvali (key words).
11. redaqcia itovebs uflebas Seasworos statia. teqstze muSaoba da Sejereba xdeba saavtoro originalis mixedviT.
12. dauSvebelia redaqciaSi iseTi statiis wardgena, romelic dasabeWdad
wardgenili iyo sxva redaqciaSi an gamoqveynebuli iyo sxva gamocemebSi.
aRniSnuli wesebis darRvevis SemTxvevaSi statiebi ar ganixileba.
Georgian Medical News
No 4 (205) 2012
Содержание:
Rainauli Z., Mekokishvili L., Robert de Petriconi
15-YEAR HISTORY OF SPONTANEOUS
APPENDICO-VESICAL FISTULA (Case report)................................................................................. 7
Davitashvili D., Beridze M., Shakarishvili R., Kiziria M., Sanikidze T.
THE ROLE OF ENDOGENOUS ANTIRADICAL PROTECTIVE SYSTEM
IN MULTIPLE SCLEROSIS........................................................................................................................ 11
Цискаришвили Н.В., Кацитадзе А.Г., Цискаришвили Ц.И.
АБСЦЕДИРУЮЩИЙ ПОДРЫВАЮЩИЙ ФОЛЛИКУЛИТ
И ПЕРИФОЛЛИКУЛИТ ГОФФМАННА
(СЛУЧАЙ ИЗ ПРАКТИКИ)....................................................................................................................... 19
Батоцыренова Х.В., Ливанов Г.А., Шестова Г.В., Цветнова Л.Д.,
Великова В.Д., Базарова В.Г.
нейропротекторные эффекты сукцинатсодержащего
антигипоксанта реамберина у больных
с токсикогипоксическим поражением головного мозга........................................ 24
Шилов В.В., Андрианов А.Ю., Васильев С.А., Батоцыренов Б.В., Лоладзе А.Т.
фармакологическая коррекция гипоксических поражений
у больных с тяжелыми формами отравлений угарным газом............................... 31
Шилов В.В., Шикалова И.А., Батоцыренов Б.В., Андрианов А.Ю.,
Васильев C.А., Лоладзе А.Т.
сравнительная оценка клинической эффективности ремаксола
и адеметионина у больных с острыми отравлениями этанолом,
осложненныМИ токсическими поражениями печени............................................... 38
Фролов В.М., Пересадин Н.А., Чхетиани Р.Б., Круглова О.В.
ЭФФЕКТИВНОСТЬ КОМБИНАЦИИ ЦИКЛОФЕРОНА И РЕАМБЕРИНА
ПРИ ЛЕЧЕНИИ БОЛЬНЫХ ХРОНИОСЕПСИСОМ.............................................................................. 44
De Sanctis V., Soliman A.T., Angastiniotis M., Eleftheriou A., Kattamis Ch., Karimi M.,
El Kholy M., Elsedfy H., Mohd Abdel Daem Mohd Yassin, El Awwa A.,
Stoeva I., Skordis N., Raiola G., Fiscina B.
INTERNATIONAL NETWORK ON ENDOCRINE COMPLICATIONS
IN THALASSAEMIA (I-CET): AN OPPORTUNITY TO GROW............................................................. 52
Маисурадзе Т.Г.
Применение препарата ДОНА - глюкозамин сульфат (Саше)
В консервативной терапии БОЛЕЗНИ ЛЕГГ-КАЛЬВЕ-ПЕРТЕСА........................................ 58
Moskalenko R., Romanyuk A., Logvin A.
INTRAFOLLICULAR PRESSURE OF COLLOID THYROID GLAND
DETERMINATE THE TYPE OF FOLLICULOGENESIS......................................................................... 67
© GMN
5
Медицинские новости грузии
cfmfhsdtkjc cfvtlbwbyj cbf[ktyb
Ratiani L., Terunashvili G., Sanikidze T.
ANTIINFLAMMATORY ACTIVITY OF LYMPHOMYOSOT
DURING CHRONIC DESEASES............................................................................................................... 73
Sukhitashvili N., Imnadze I., Tabaghua G., Gogilashvili Q., Amiranashvili I.
CHARACTERIZATION OF ORAL ULCER
AND PATHOLOGICAL SCAR IN NUDE MICE MODEL........................................................................ 82
6
Georgian Medical News
No 4 (205) 2012
– polisulfatirebuli gag-is ganyofilebaSi mkurnalobis procesSi
gamoyenebis gamocdileba. zemoaRniSnulidan gamomdinare preparati dona
CarTuli iqna pertesis daavadebis
kompleqsuri konservatiuli Terapiis
sqemaSi, romlis mixedviTac mkurnaloba Cautarda pertesis daavadebiT sxvadasxva stadiaSi myof 5-dan 14 wlamde
300 bavSvs. mkurnalobis procesSi da
misi damTavrebis Semdgom (5-6 weli)
dakvirvebis Sedegad ar gamovlinda
hipermgrZnobelibisa da gverdiTi
movlenebis arc erTi SemTxveva. 90-92%
aRniSna mkurnalobis dadebiTi Sedegi.
rentgenologiurad dadasturebulia
dazianebuli barZayis Zvlis yelisa
da Tavis Zvlovani da xrtilovani
qsovilebis reparaciuli procesebis
daCqareba, aRdgena, rac avtors safuZvels aZlevs rekomendacia gauwios
preparat dona - glukozamin sulfats
(saSe) -1500, raTa igi gamoyenebul iqnes
leg-kalve-pertesis daavadebis kompleqsur konservatiul mkurnalobaSi 5-dan
14 wlamde asakis bavSvebSi, rogorc
struqturul-modificirebuli, rbili
moqmedebis preparati.
INTRAFOLLICULAR PRESSURE OF COLLOID THYROID GLAND DETERMINATE
THE TYPE OF FOLLICULOGENESIS
Moskalenko R., 1Romanyuk A., 2Logvin A.
1
Sumy State University, Medical institute, Department of Pathomorphology;
2
Sumy State University, Department of Processes and Equipment
of Chemical and Petroleum-Refineries, Ukraine
1
The problem of morphogenesis influence of pressure of colloid on thyroid gland is not researched
enaugh in the scientific literature. In 1982 C.
Garbi et al demonstrated the fact of the follicular
colloid pressure in following way: after puncture
of the follicles by the needle lumen compressed
and follicular cells became cuboids [4]. On absence of polarity (basal differentiation) in thyrocytes culture which could’t form follicles was
also established [6]. Recent studies have found
that vigorous expression of thyroperoxydase,
natriun-iodium simporter in follicles begin only
after the appearance of colloid [5].
© GMN
The aim: research mechanism of folliculogenesis
in the thyroid gland under the influence of heavy
metals salts.
Matherial and methods. The research of the
intraluminal pressure influence of the colloids
on folliculogenesis was conducted on 24 white
Wistar line rats (6 months age, 200-250 g weight).
During the experiment, laboratory animals
were maintained in accordance with regulations
adopted by the European Convention for the
Protection of vertebrate animals, which we used
for experiment and research tasks (Strasbourg,
67
1986), “General ethical rules of experimentation
on animals”, and approved by the Congress of
Bioethics (Kyiv, 2001) and the Law of Ukraine
“On protection of animals from cruelty» № 3477IV dated 21.02.2006.
6 animals in each group were taken for the experiments, access to water was free. To break
thyroid morphofunctional state of equilibrium
the experimental rats received a combination of
heavy metals salts (increased amount of zinc,
copper, iron, manganese, lead, chromium).
Experimental animals of both series are divided
into groups according to the derived set of xenobiotics. 1-first group were the control rats receiving distilled water. Animals of group 2 received
distilled water with a combination of heavy metals salts: zinc (ZnSO4 x 7H2O) - 5mg/l, copper
(CuSO4 x 5H2O) - 1 mg/l, iron (FeSO4) - 10 mg/l,
manganese (MnSO4 x 5H2O) - 0,1 mg/l lead (Pb
(NO3)2) - 0,1 mg/l, chromium (K2Cr2O7) - 0,1
mg/l. To study the dynamics of morphological
changes animals were removed from experiments
on 30 and 60 days.
After the experiment, animals were decapitated
under ether anesthesia, thyroid was received,
and then weighed on analytical scales “VLA200” (Ukraine) to within 1 mg. Organometry of
thyroid consisted of measuring the length, width
and thickness of right lobe by using caliper,
measuring thyroid volume by a dial test tubes
– according to the volume of liquid forced out.
Morpho-functional state of thyroid gland was
studied using histological, histochemical, morphometric methods.
Histological and histochemical methods. For
histological examination the right lobe of thyroid
was taken and fixed it in 10% neutral formalin
solution or Bouins Solution within 24 hours.
Making paraffin blocks was performed by the
standard technique. By microtomes paraffin serial
sections 7-9 mm thick were performed, which
passed through the center, and subcapsular minor
glands, painted with hematoxylin-eosin stain.
68
Медицинские новости грузии
cfmfhsdtkjc cfvtlbwbyj cbf[ktyb
Dyeing methods of van Gizon, Gomorry, PASreaction were also used.
Morphometric method. Suitable histological
preparations were studied and photographed using a digital image output system «SEO Scan ICX
285 AK-F IEE-1394” (Ukraine). In an environment of morphometric computer program «SEO
Image Lab 2,0» the analysis of micropreparation image was performed the average values
of morphometric parameters were determined:
bigger and smaller diameters of follicles, height
of thyrocytes, determined optical density of the
colloid follicles.
Mathematical methods. The modeling of influence salts of heavy metals on tension distribution
in the follicle wall was carried out with the help of
«Cosmos Works». To calculate the tension value
in the follicle wall, the mathematical algorithm
was used, which was borrowed from thin-walled
shell theory [3]. The tension in a spherical shell
which is under the internal pressure Р is determined by the formula:
(1)
where Р – pressure in a follicle, Pа; R – follicle
radius , m; h – thickness of a follicle wall, m.
There is an expression for the spherial calotte in
the case of homogeneous deformation:
(2)
where Н – altitude of a segment layer, m;
(3)
where Н=kR, H0=kR, where k – constant.
In the case of biaxial tension (ε1=ε2=ε) the material stands the shearing stress:
(4)
Georgian Medical News
No 4 (205) 2012
where ε - material unit elongation; Е – material
elastic modulus, Pа; μ - Poisson’s ratio.
Under the condition:
ε=R/R0-1, (5)
The formula can be rewritten:
(6)
So we get the formula of dependence of P and
R:
(7)
While researching function P(R) at maximum we
get formulae to evaluate critical parameters:
,
(8)
The obtained data were processed statistically on
a personal computer using application package.
The discrepancy validity between experimental
and test data were estimated with the help of
Student‘s t test. The admissible error is less than
5% (р<0,05)
Results and their discussion. Based on the
theory of thin shells and the results of morphometric study of intact groups of mature rats,
which were influenced by a combination of heavy
metals salts, the mathematical model to determine
the level of tension in the wall of follicles was
deduced. The value of the vascular skeleton of
microvasculature perifocal blood capillary for
mechanical strength of thyroid parenchyma is
extremely important, as they closely braid all
follicles, forming a single extended structure.
The interfollicular connective tissue contains
many layers of textures capillaries. Each follicle
is surrounded by a capillary net. Mono-layer
capillary network consist of anastomosing blood
microvessels. Capillaries diameter is uniform.
Each capillary loop contacts with 4-5 neighbor© GMN
ing loops around each follicle and on each side
there are 5-6 capillaries. Therefore, in subsequent
calculations Poisson ratio typical of vessels was
used.
During the study of a maximum tension in the
wall of follicles of rats of the control group,
where the rapture might happen, it has been found
that the importance index of 30 day observation
period was 0.051546 MPa, and after 60 days 0.047705 MPa. After 30 days of the salts of heavy
metals influence the critical stress in the wall of
follicles decreased to 24.37% after 60 days of the
experiment - 25.65%.
Thus, the maximum tension that can withstand
the wall depends on the size of the follicle diameter and height of follicular endocrinocytes (wall
thickness) - the larger follicle size and smaller
follicular endocrinocytes height, the less stress
can the follicle wall with stand.
Experimental results showed that with increasing
follicle size tension in their side is also increased,
but after reaching the critical value its gradual
decline occurs. We can assume that, to prevent the
rupture of the follicle membrane physiological or
compensation-adaptive mechanisms are used. To
simulate the influence of internal pressure on its
wall the following methods simplification were
selected: follicle has correct elliptical shape and
uniform hydrostatic pressure affects the entire
surface of the follicle. Polyethylene was taken as
this materials is most highly similar to the studied
biological tissue. Pressure P = 1 atm was selected
for the simulation. Using computer simulation of
stress and strain of thin membrane of thyroid follicle by “Cosmos Works” it was established that the
elliptical form of follicle wall tension in the follicle
was this: more tension appears on the longitudinal
section of the ellipse - to 0,4 Nm/m2, and in cross
section the tension is 0.3 N/m2 (Fig.).
As a result of the influence of combinations of
heavy metals salts on the follicular apparatus thyroid hypoactivity developed, which manifested in
accumulating colloid and changing its tinctorial,
69
physical and chemical properties, reducing the
height of follicular epithelium, follicles increase
Медицинские новости грузии
cfmfhsdtkjc cfvtlbwbyj cbf[ktyb
in diameter, which also reflected the increasing
tension in the wall of the follicle.
Fig. Stress distribution curve in the ellipsoid shell. When considering the cross-section shell red
tint shows that more tension occurs on the longitudinal section of the ellipse,
and in cross-section the tension decreases
Lots of colloid with greater optical density have
greater physical and chemical density and viscosity, areas with lower optical density – have
density and viscosity lower. Research of the colloid using morphometric software «SEO Image
Lab 2,0» automatic mode selection confirmed
that areas with different density may indicate
uneven distribution of colloid density. Uneven
colloid concentrations are easily visible on
mounts stained by histochemical PAS-reaction.
When retained in the cavity of the follicle colloid
increases its condense, saturated hydrocarbon
component. PAS - reagent paints concentrated
colloid in purple-pink color. Uneven distribution
of pressure inside the follicle may be a significant
factor for the division the follicles.
Based on the opinion under which folds and follicle epithelial buds or bands are immersed in the
follicle lumen in the direction of least resistance,
it is clear active participation of intrafollicular
pressure in the process of folliculogenesis becomes clear. The work of N.P. Fedchenko covers
the mechanism of folliculogenesis in the central
areas of the thyroid gland in conditions of prolonged hypercalcemia fragmentation follicles by
ingrowed strands of connective tissue as vectors
of force was described [1]. In our opinion, the
70
direction of division and fragmentation follicles
is specified by the different gradients of pressure inside the follicles. We can assume a direct
relationship between functional activity of follicular epithelium and the predominant type of
folliculogenesis,as it is known that in particles of
peripheral areas of thyroid the morphofunctional
activity of thyrocytes are lower.
Based on the results of our research, we can speak
about the important role of mechanical factors,
physical and chemical processes of colloid in
the central and peripheral folliculogenesis. We
can assume a direct relationship between functional activity of follicular epithelium and the
predominant type of folliculogenesis. It is known
that in particles of the peripheral areas of thyroid
the morphofunctional activity of thyrocytes are
lower and intrafollicular pressure, respectively,
higher than in central areas, and that makes a
difference in the directions of vectors of foldes
of follicular epithelium [2]. Thus, in peripheral
areas because of greater pressure and resistance
of intrafollicular colloid fragmentation occurs at
acute angles and it leads to separation of smaller
follicles, even to microfolliculies formation in the
same parent follicle wall. In the central areas of
the parenchyma of thyroid follicular epithelium
Georgian Medical News
No 4 (205) 2012
functional activity is higher, more rarefied and
colloid, respectively follicles are fragmenting
more even - in the course of the central folliculogenesis 2-3 flattened follicles are formed.
Thus, the results allow to conclude that there
is a significant role of intrafollicular colloidal
pressure in the process of folliculogenesis. It is
determined that in the wall of the follicle of elliptic form higher tension arises in longitudinal
section of the ellipse, and in cross-section the tension decreases. Analyzing the research results, we
can assume that the difference between peripheral and central folliculogenesis occurs because
of different amounts of intrafollicular pressure,
which again are derived from different functional
activity of the follicular epithelium.
REFFERENCES
1. Федченко М.П., Федченко М.М., Шпонька
І.С. Теоретичні та патоморфологічні аспекти
канцеро- і антиканцерогенезу в щитоподібній
залозі. Морфологія 2007; 1(1): 25-28.
2. Федченко Н.П., Бондаренко А.А., Гарец
В.И. Современные аспекты структурнофункциональной организации щитовидной
железы.
Український
морфологічний
альманах 2008; 6(1): 161-164.
3. Стороженко В.Я. Розрахунок тонкостінних
апаратів (теорія і практика). Суми: Вид-во
Алан-прес; 2002: 210.
4. Garbi C., Wollman S.H. Ultrastructure and
some other properties of inverted thyroid follicles in suspension culture. Exp Cell Res. 1982;
138(2): 343–353.
5. Szinnai G., Lacroix L., Carre A. et al. Sodium/
iodine symporter (NIS) gene expression is the
limiting step for the onset of thyroid function
in the human fetus. J Clin Endocrinol Metab.
2007; 92: 70-76.
6. Wollman S.H., Nitsch L., Gabri C. and
Tacchetti C. Aspects of control of polarity of
thyroid epithelial cells in follicles in suspension culture. In: Thyroglobulin – the prothyroid
hormone. Ed. by Eggo M.C., Burrow G.N. New
York: Raven Press; 1985: 235-241.
© GMN
SUMMARY
INTRAFOLLICULAR PRESSURE OF COLLOID THYROID GLAND DETERMINATE
THE TYPE OF FOLLICULOGENESIS
Moskalenko R., 1Romanyuk A., 2Logvin A.
1
Sumy State University, Medical Institute, Department of Pathomorphology; 2Sumy State University, Department of Processes and Equipment
of Chemical and Petroleum-Refineries, Ukraine
1
The problem of colloid pressure of influence on
the thyroid gland folliculogenesis is not clear
enough in the scientific literature. The purpose
of this study was to investigate the mechanism
of folliculogenesis in the thyroid gland under
the influence of salts of heavy metals combinations on the organism. The research was
conducted on 24 white adult rats. To study the
dynamics of morphological changes animals
were removed from experiments on 30 and
60 days.
Based on the theory of thin shells and the results
of morphometric study of both groups of adult
rats, which were influenced by a combination
of heavy metal salts, the mathematical model
to determine the level of tension in the wall of
follicles was deduced. As a result of the influence of combinations of heavy metal salts on
the follicular apparatus thyroid hypoactivity
developed, which manifested in accumulating
colloid and changing its tinctorial, physical and
chemical properties, reducing the height of follicular epithelium, follicles increase in diameter,
which also reflected the increasing tension in the
wall of the follicle.
Thus, the results allow to conclude that there
is a significant role of intrafollicular colloidal
pressure on the process of folliculogenesis. It
is determined that in the wall of the elliptic
form follicle higher tension arises in longitudinal section of the ellipse, and in cross-section
the tension decreases. Analyzing the research
71
results, we can assume that the difference between peripheral and central folliculogenesis
occurs because of different amounts of intrafollicular pressure, which again are derived from
different functional activity of the follicular
epithelium.
Keywords: thyroid gland, folliculogenesis, intrafollicular pressure, colloid, heavy metal salts.
РЕЗЮМЕ
ВНУТРИФОЛЛИКУЛЯРНОЕ ДАВЛЕНИЕ
КОЛЛОИДА ЩИТОВИДНОЙ ЖЕЛЕЗЫ
ОПРЕДЕЛЯЕТ ТИП ФОЛЛИКУЛОГЕНЕЗА
Москаленко Р.А., 1Романюк А.М.,
2
Логвин А.В.
1
Сумский государственный университет,
Медицинский институт, кафедра патоморфологии; 2Сумский государственный университет, кафедра оборудования химических и
нефтегазовых производств, Украина
1
Проблема влияния давления коллоида на
фолликулогенез в щитовидной железе является малоизученной. Целью настоящего
исследования явилось изучение механизма
фолликулогенеза в щитовидной железе в
условиях влияния на организм комбинации
солей тяжелых металлов. Исследование было
проведено на 24 белых крысах-самцах репродуктивного возраста. Для исследования динамики морфологических изменений животных
выводили из эксперимента на 30 и 60 сутки
путем декапитации под эфирным наркозом.
Основываясь на теории тонкостенных оболочек и результатах морфометрического
исследования щитовидной железы животных контрольной и подопытной групп,
была построена математическая модель для
определения напряжения в стенке фолликула. В результате влияния комбинации солей
тяжелых металлов на фолликулярный аппарат в щитовидной железе развивается гипо72
Медицинские новости грузии
cfmfhsdtkjc cfvtlbwbyj cbf[ktyb
функциональное состояние, которое проявляется в накоплении коллоида и изменении
его тинкториальных и физико-химических
свойств, уменьшении высоты фолликулярного эпителия, увеличении диаметра фолликулов, что также отображает повышение
напряжения в стенке фолликула. Полученные результаты позволяют сделать вывод о
значительной роли внутрифолликулярного
давления коллоида в процессах фолликулогенеза. Установлено, что в стенке фолликула
эллипсоидной формы более высокое напряжение возникает по продольному разрезу
эллипса, а в поперечном разрезе напряжение
уменьшается. Анализируя результаты исследования, можно допустить, что разница
между периферическим и центральным фолликулогенезом возникает вследствие разных
значений внутрифолликулярного давления,
что, в свою очередь, является производным
от различной функциональной активности
фолликулярного эпителия.
reziume
folikulisSida koloiduri wneva
gansazRvravs folikulogenezis ti ps
farisebr jirkvalSi
1
r. romanenko, 1a. romaniuki,
2
a. logvini
1
sumis saxelmwofo universiteti,
samedicino instituti, paTomorfologiis kaTedra; 2sumis saxelmwofo universiteti, navTobis warmoebisa da qimiuri aRWurvilobis kaTedra, sumi, ukraina
farisebri jirkvlis morfogenezSi koloiduri wneviT gamowveuli
cvlilebebi naklebadaa Seswavlili.
kvlevis mizans warmoadgenda farisebri jirkvlis folikulogenezis
meqanizmis gamokvleva organizmze mZime liTonebis marilebis zemoqmedebis pirobebSi. gamokvleva Catarda
Georgian Medical News
No 4 (205) 2012
24 reproduqciuli asakis Wistar-is
xazis mamr-virTagvaze. morfologiuri cvlilebebis gamokvlevisaTvis
cxovelebi gamogvyavda cdidan dekapitaciiT eTeris narkozis qveS, masalas
viRebdiT 30-e da me-60 dRes.
Txelkedliani struqturis Teoriaze
dayrdnobiT da farisebri jirkvlis
gamokvlevis Sedegad sakontrolo
da sacdel jgufebSi Seiqmna maTematikuri modeli folikulis kedlis
daZabulobis gansazRvrisaTvis.
mZime liTonebis marilebis kombinaciis zemoqmedebis Sedegad farisebri
jirkvlis folikulur struqturaSi
dafiqsirda hipofunqciuri mdgomareoba koloidebis dagrovebiT,
misi fizikur-qimiuri Tvisebebis
cvlilebis, folikulis epiTeliumis
simaRlis Semcirebis da folikulTa
diametris gazrdiT, rac gamovlinda
folikulis kedlis daZabulobis
momatebiT. am faqtze dayrdnobiT
SesaZlebelia daskvnis gamotana imis
Sesaxeb, rom folikulis sanaTurSi
koloidis wneva mniSvnelovan rols
asrulebs folikulogenezSi. dadgenilia, rom elifsuri formis folikulis kedlis sigrZiv Wrilze viTardeba meti daZabuloba, vidre ganivze,
sadac es maCvenebeli iklebs.
kvlevis Sedegebis analizis safuZvelze, avtorebi SesaZleblad miiCneven
ivaraudon, rom sxvaoba periferiuli
da centraluri folikulogenezis
meqanizmebSi warmoiqmna folikulSida wnevis gansxvavebuli maxasiaTeblebis gamo, rac, Tavis mxriv, damokidebulia folikuluri epiTeliumis
funqciuri aqtivobis gansxvavebuli
maCveneblebiT.
ANTIINFLAMMATORY ACTIVITY OF LYMPHOMYOSOT
DURING CHRONIC DESEASES
1,2
Ratiani L., 2Terunashvili G., 1,2Sanikidze T.
Tbilisi State Medical University; 2LTD Clinic “Healthy Women”, Tbilisi, Georgia
1
Cells inside the living multicellular organism keep
constantly interconnected through various signals
(including electrical, chemical, mechanical, heat
etc.), thus building up organized and balanced
system. Under conditions of any pathological
process (backgrounder by hypoxia and ischemia,
which, in turn, develop as a consequence to the
influence laid by toxic metabolites of the cell),
intercellular links may disrupt, or adaptive compensative reactions may ensue. The latter support
© GMN
to non-controlled hyperactivation of cellular
metabolism, which leads to accumulation of the
toxic metabolites, intoxication and further derangement inside the cell, ultimately resulting in
deepening of the pathological process. Functional
failure of the tissues grow deeper simultaneously
with increasing number of disturbed cells, which
makes pathological process irreversible, thus providing the development of chronic inflammatory
pathological process in the body. Inflammation is
73

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