Cryptococcus neoformans Chemical Amount Urea 21.5 mM KH2PO4 10 mM MgSO4*7H2O 1.2 mM MnCl2*4H2O 1 µM FeSO4*7H2O 10 µM ZnSO4*7H2O 10 µM CuSO4*5H2O 1.2 µM CaCl2*2H2O 100 µM Thiamin 2 mg/l pH 6.0 Select agar 15 g/l After autoclaving D(+) biotine 10 µg/l Ref: Cherniak, R., E. Reiss, and S.H. Turner, A galactoxylomannan antigen of Cryptococcus neoformans serotype A. Carbohydr. Res., 1982(103): p. 239‐250.