Introduction to Enzyme
Function Lab
Period 2 Results
Look at your results. In
which test tubes did you
observe bubbles or
balloon inflation?
Period 2 Results (Glucose Control)
Period 2 Results (Untreated Lactase)
What was in the
untreated lactase test
tubes?
What would you expect
to happen in this test
tube?
How does that compare
to what we actually saw?
What could this tell us
about the enzyme?
Period 2 Results (Temperature)
Test tube 5 with the inflated balloon
corresponds to the lactase enzyme
treated at 40°C. What is 40°C in
degrees Fahrenheit?
Why might this temperature have
worked and the others failed?
How does temperature affect the
lactase and lactose in this test tube?
Period 2 Results (pH)
pH 6
pH 6
Lab Summary
What evidence from your experiment indicated
whether lactase was functioning?
What were the controls in the experiment? What
information did they provide?
What evidence from you experiment indicated whether lactase was functioning?
Overall Results
I conducted the
temperature portion
of the experiment
on my own and got
these results.
What does each
test tube tell us?
Why did we need so
many controls?
Overall Results
Balloon
Number
1
(Yeast)
2
(Glucose
control)
3
(Lactose
control)
4
(Lactase
control)
5
(40°C)
6
(60°C)
7
(80°C)
8
(100°C)
9
(Untreated
lactase)
Observation
none
yes
none
none
yes
none
none
none
some
Balloon
Number
1
(Yeast)
2
(Glucose
control)
3
(Lactose
control)
4
(Lactase
control)
5
(pH 4)
6
(pH 6)
7
(pH 8)
8
(pH 10)
9
(Untreated
lactase)
Observation
none
yes
none
none
none
yes
yes
none
some
Period 5 Results
Look at your results. In
which test tubes did
you observe bubbles or
balloon inflation?
Period 5 Results (Glucose Control)
Why were the balloons on the glucose control test tubes consistently
inflated?
Period 5 Results (Untreated Lactase)
What was in the untreated
lactase test tubes?
What would you expect to
happen in this test tube?
How does that compare to
what we actually saw?
What could this tell us
about the enzyme?
Period 5 Results (pH)
Period 2 Results (pH)
pH 6
pH 6
Overall pH Results
The pH results for Period 5 consistently
showed balloon inflation in all four test
tubes.
Do those results make sense?
Why do you think this happened?
What would you expect the results to be
and why?
Period 5 Results (Temperature)
This image shows a
small amount of balloon
inflation in each of the
temperature-treated test
tubes.
Did anyone notice if one
particular temperature
was inflating more than
another?
Period 2 Results (Temperature)
Test tube 5 with the inflated balloon
corresponds to the lactase enzyme
treated at 40°C. What is 40°C in
degrees Fahrenheit?
Why might this temperature have
worked and the others failed?
How does temperature affect the
lactase and lactose in this test tube?
Lab Summary
What evidence from your experiment indicated
whether lactase was functioning?
What were the controls in the experiment? What
information did they provide?
What evidence from you experiment indicated whether lactase was functioning?
Overall Results
I conducted the
temperature portion
of the experiment
on my own and got
these results.
What does each
test tube tell us?
Why did we need so
many controls?
Overall Results
Balloon
Number
1
(Yeast)
2
(Glucose
control)
3
(Lactose
control)
4
(Lactase
control)
5
(40°C)
6
(60°C)
7
(80°C)
8
(100°C)
9
(Untreated
lactase)
1
(Yeast)
2
(Glucose
control)
3
(Lactose
control)
4
(Lactase
control)
5
(pH 4)
6
(pH 6)
7
(pH 8)
8
(pH 10)
9
(Untreated
lactase)
Observation
Balloon
Number
Observation