The Death Valley Regional Flow System: A Fault-controlled Oasis for Deep Life Beneath the Mojave Desert Duane P. Moser, Desert Research Institute, Las Vegas, NV, USA Concept: The Death Valley Regional Flow System (DVRFS) of Nevada and California, USA, is a unique site for ICDP drilling at the nexus of life, geology, hydrology, and societal interests. 3D Geological Context: Located in the Basin and Range physiographic province of the Western United States, the DVRFS is a deep, fracture-controlled aquifer that encompasses ~100,000 km2 of mountain ranges (up to 3,600 m above sea level) and valleys which can reach below sea level (e.g. Death Valley, at -86 m, the lowest point in North America [1]). Climate is controlled by altitude: with the highest peaks receiving ~100 cm/y of precipitation and Death Valley, the hottest place on Earth [2] and driest place in North America, a mere ~4 cm/y [3]. DVRFS geology is both active (Nevada is the US’ 3rd most tectonically-active state [4]) and complex; recording tectonic and sedimentary (marine and continental), metamorphic, and intrusive igneous histories from over 2 Ga. Geologically-significant events include: 1) formation of thick Paleozoic (~550 Ma) marine carbonates that underlie most of the DVRFS, 2) extrusion of a broad Tertiary (23 – 2.6 Ma) volcanic plateau in the central DVRFS, and 3) crustal extension, from ~16 Ma to present, that created the Basin and Range and Death Valley (~2 – 3 Ma, [5]). This region has thin crust (e.g. ~17 – 25 km under Death Valley [6]) and extensive faulting (e.g. horst/graben structures). The valleys (grabens) are partially filled with sediment and sometimes lakes. The largest, Lake Manly, filled Death Valley from 240,000 to 10,000 years BP [7]. DVRFS faults are thought to control groundwater flow to thousands of m-depth and over hundreds of km; from montane recharge areas to large-discharge springs on the low-elevation valley floors. This “interbasin flow” concept [8-11] is controversial [12-14] and a better understanding of fault structure would enable improved predictions of the amount, age, and flow paths of regional groundwater. As a result of societal pressures for water, the potential for contamination migration from underground nuclear weapons testing on the Nevada National Security Site (NNSS) and a proposed nuclear repository (Yucca Mountain), the deep regional hydrology of the DVRFS is possibly the best understood in the world, and 3-D groundwater flow models of ever-increasing sophistication are being developed by the US Geological Survey (USGS) and Department of Energy (USDOE). Most significantly, a comprehensive DVRFS hydrologic model with 1,500 m grid cell spacing (194 rows, 160 columns and 16 layers, surface to 6 km below sea level) is being developed by the USGS. This model incorporates several embedded models, including the 50-m-resolution Southern Amargosa eMbedded Model (SAMM), which specifically covers the main area targeted in this proposal. What is Known - Deep Life): To date, fluids from ~sixty-five DVRFS wells (100 – 1300 m) and regional springs have been analyzed for aqueous geochemistry and microbial community structure by our laboratory using next-gen and PCR-amplified 16s rRNA gene libraries. Microbial communities, consistent with subsurface origins [15-17], have been detected in most (e.g. the “dry hole” scenario is unlikely). Results have confirmed dissolved oxygen penetration to > 1300 m depth in the DVRFS recharge zone [16, 18, 19] and microbial populations dominated by proteobacteria and Thaumarchaeaota. Suboxic/anoxic wells sample deeper, hotter sources, dominated by Nitrospirae, Firmicutes, and methanogens (Eurychaeaota). “Dark matter” phyla (e.g. OP3) and sequences closely related to Candidatus Desulforudis audaviator are also common (the bacterial population in 880-m-deep well BLM-1 (see below), for example, is ~50% C. Desulforudis spp.). Our culture collection features sulfate-reducing and peptide-utilizing Firmicutes related to Desulfotomaculum and C. D. audaxviator (~ 91% identity). Proposed Sites: Our group has developed valuable professional contacts over the past decade that support consideration of dozens of relevant sites. We a proposing three for evaluation: 1) Amargosa Valley, NV. Private quarry lands with existing drill pads enable permit-free access to the “Gravity Fault”, which controls discharge of deep regional aquifer water to Ash Meadows springs and Devils Hole (home to endangered species, e.g. Devils Hole Pupfish). The fault may (or may not) protect these treasures from proposed groundwater mining. Other sites, accessing the Lower Carbonate Aquifer (“LCA”, BLM-1 area), boast existing drill pads and a relatively streamlined BLM permitting process. 2) Oasis Valley, NV (Spicer Ranch). Private lands enable permit-free access to DVRFS recharge zone volcanic strata and probably very deep underlying Paleozoic carbonates. 3) Death Valley, CA. Managed by the US National Park Service (NPS), permitting may require several years, but drilling is feasible for the floor of Death Valley (e.g. ~ 2,800 m of saline deposits [6]) and the seismically-active [20, 21] Furnace Creek Fault Zone, where several drill pads already exist (e.g. Echo Canyon and the Nevares Spring Mound). DCO and Deep Life Community (DLC) Goals: The proposed sites support DLC goals 1 and 2), characterization of the diversity and distribution of deep life as it relates to the carbon cycle and interactions between deep life and carbon cycling on Earth. In particular, a better understanding of deep life across the LCA and shallower aquifers will facilitate an understanding of sources and sinks of organic C and resolution of a persistent 14C age-dating discrepancy (14C DIC much older than 14C DOC (~30,000 y vs. ~3,000 y [22]). 2) Given a local geothermal gradient of at least 33oC/km [23, 24] (BLM-1 produces anoxic 61oC water at 880 m depth, e.g. ~ 40oC per km), zones enabling the determination of the environmental limits of deep life (e.g. temperature, salinity) will be relatively easily accessed: the Death Valley salt pan, for example, is largely halite and 120oC isotherm should be surpassed at << 3 km depth. ICDP Criteria: Global Criterion: This is both the most arid and fastest-growing region of North America. This juxtaposition of factors must certainly represent a “world class problem”. International Criterion: Our proposed study sites inform a range of geologically-important provinces and rock types (e.g. continental dolomite, extrusive igneous). Need for Drilling: The geology and hydrology of this region is controlled by faults, the most important of which are invariably obscured by valley fill. One controversial topic involves whether or not the Death Valley Salt Pan receives DVRFS flow. Drilling is the only means to test geophysical data. The drilling of hazardous zones (e.g. active faults) is a current priority of ICDP. Depth and Cost/Societal Needs: The USGS, NPS, and DOE are currently in discussions concerning a new drilling project that would explore the Furnace Creek Fault Zone. It is conceivable that these agencies would welcome new partners. High temperatures occur at shallow depths across the DVRFS, reducing the cost of drilling to the “biotic fringe”. Contractors with relevant expertise have been working in the basin for decades. Active Processes Criterion: See Geological Context. Supplement: Responses to Suggested Workshop Priorities. Potential for abiogenic H2: Deep sites from this system have already been characterized for H2 and it is measurable in anoxic sites (<0.01 vol%). In addition, aside from manmade contamination, which produces large amounts of H2 via activity of up to 10s of millions of pCi/L tritium, at least some uncontaminated wells have U concentrations exceeding the drinking water MCL (e.g. ~15 pCi/L gross alpha) suggesting H2 production potential. Potential to transect depth limit for life: Depending upon location, the 120oC Isotherm is within reach of common drilling technology across portions of the DVRFS. The Geothermal gradient in the Death Valley region is known to be very high, (e.g. published values of about 33oC/km [23, 24]). This gradient will likely be even higher on the floor of Death Valley. Previously unexplored but globally important subsurface environment: Continental carbonate rocks make up about 20% of the Earth’s land surface [25]. The proposed sites enable the exploration of a wide range of rock types with shared hydrologic context (e.g. volcanics, metamorphic, and sedimentary rocks in the same hole). Potential for other disciplines (i.e. geophysics, hydrology, geology) to piggy-back onto our project: Essentially all of the work conducted within the Death Valley Flow System to date has been supported by these disciplines. Microbiology is the fresh face here. Numerous discussions with workers in these disciplines indicate a very high degree of interest in partnering. 3D structure and hydrology: The geologic structure and hydrology of the proposed region is among the best understood of any in the world (Wayne Belcher, USGS, pers. comm.). DVRFS 3 dimensional hydrologic models are extremely detailed, ground-truthed, and extend to great depths (6 – 10 km below sea level). This factor may represent the greatest advantage embodied by these sites. The microbially pristine nature of the site, Although extensive drilling has been conducted, very few of the wells within the regional flow system have been use for production. Other activities, such as hydrocarbon production, have not occurred. Outside of urban areas, the region is mostly unsettled and Las Vegas is in a different hydrologic basin and gets almost all of its water from the Colorado River. There is no significant mining that impacts the regional flow system. Although large amounts of radiologic contamination are present on the NNSS, none is anywhere near the proposed sampling areas and DOE’S Underground Test Area Program (UGTA) models predict that this contamination will not reach these zones for 100s of years. Long-term accessibility to the borehole(s) afterwards for groundwater sampling and in situ experiments. The precedent of hundreds of monitoring wells already existing on the NNSS and elsewhere bode well in this regard. Well BLM-1 is already established as a long-term science well by its stewards (Inyo Co, CA and the NPS) and long-term microbial substrate incubations already underway by NASA Astrobiology Institute. Potential Proponents (confirmed interest): Richard Friese, Hydrologist, Death Valley National Park; Wayne Belcher and John Wilson , US Geological Survey, Las Vegas; Michael King, the Hydrodynamics Group, LLC, Redmond, WA. Levi Kryder, Nye County Nuclear Waste Repository Program Office, Pahrump, NV. 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