4
Certificate of Analysis
The Certificate of Analysis (CofA) provides detailed quality control information for each
product. The CofA is available on our website at www.lifetechnologies.com/cofa, and it
is searchable by product lot number, which is printed on each box.
Limited Use Label License No. 358: Research Use Only
The purchase of this product conveys to the purchaser the limited, non-transferable right
to use the purchased amount of the product only to perform internal research for the sole
benefit of the purchaser. No right to resell this product or any of its components is
conveyed expressly, by implication, or by estoppel. This product is for internal research
purposes only and is not for use in commercial applications of any kind, including,
without limitation, quality control and commercial services such as reporting the results
of purchaser’s activities for a fee or other form of consideration. For information on
obtaining additional rights, please contact [email protected] Out Licensing,
Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008.
Limited Product Warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set
forth in the Life Technologies’ General Terms and Conditions of Sale found on
Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If
you have any questions, please contact Life Technologies at
www.lifetechnologies.com/support.
Gateway® pCMV•SPORT6, Not I-Sal I Cut
Cat. no.
12209-011
Size
5 μg
Pub. Part no. 12209011.pps
MAN0001048
Conc. 50 μg/mL
Store at −30°C to −20°C
Rev. Date 27 January 2012
Description
The Gateway® pCMV•SPORT6 Not I-Sal I Cut vector is designed for
directional cloning of cDNA that contain Not I and Sal I-compatible termini.
The vector contains attB sites so that the clones from the resulting cDNA
library can be used in conjunction with the Gateway® Cloning Technology
(Hartley, 2000). The Gateway® Cloning Technology is a novel universal
system for cloning and subcloning DNA segments, facilitating gene functional
analysis, and protein expression. The Gateway® BP Reaction is based on the
site-specific recombination system of bacteriophage lambda, and can be
represented as follows:
attB1-gene-attB2 × attP1-ccdB-attP2 ⇒ attL1-gene-attL2 × attR1-ccdB-attR2
(cDNA in pCMV•SPORT6) (Donor Vector)
(Entry Clone)
(by-product)
Important Features
©2012 Life Technologies Corporation. All rights reserved. The trademarks
mentioned herein are the property of Life Technologies Corporation or their
respective owners
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL
WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED,
INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR
A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED
BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S)
BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY
STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH
OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE
USE THEREOF.
For support visit www.lifetechnologies.com/support or
email [email protected]
• Gateway® pCMV•SPORT6 Vector has been digested with Sal I (5′) and Not I
(3′) for construction of cDNA libraries containing Not I and Sal I termini.
cDNA (containing Kozak and ATG) cloned into this vector can be
expressed in mammalian cells.
• Gateway® pCMV•SPORT6 Vector contains a CMV promoter upstream of
the attB1 site followed by restriction endonuclease sites and attB2.
Downstream of the attB2 sequence is the SV40 small t-intron and
polyadenylation signal.
• This vector contains the SP6 and T7 RNA polymerase promoters flanking
the attB1 and attB2 sites, respectively. The cDNA can be sequenced from
either direction using suitable primers. The vector contains a pUC origin of
replication, an F1 intergenic region, and an ampicillin resistance marker.
Intended Use: For research use only.
Not intended for any animal or human therapeutic or diagnostic use.
www.lifetechnologies.com
4
Certificate of Analysis
The Certificate of Analysis (CofA) provides detailed quality control information for each
product. The CofA is available on our website at www.lifetechnologies.com/cofa, and it
is searchable by product lot number, which is printed on each box.
Limited Use Label License No. 358: Research Use Only
The purchase of this product conveys to the purchaser the limited, non-transferable right
to use the purchased amount of the product only to perform internal research for the sole
benefit of the purchaser. No right to resell this product or any of its components is
conveyed expressly, by implication, or by estoppel. This product is for internal research
purposes only and is not for use in commercial applications of any kind, including,
without limitation, quality control and commercial services such as reporting the results
of purchaser’s activities for a fee or other form of consideration. For information on
obtaining additional rights, please contact [email protected] Out Licensing,
Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008.
Limited Product Warranty
Life Technologies Corporation and/or its affiliate(s) warrant their products as set
forth in the Life Technologies’ General Terms and Conditions of Sale found on
Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If
you have any questions, please contact Life Technologies at
www.lifetechnologies.com/support.
©2012 Life Technologies Corporation. All rights reserved. The trademarks
mentioned herein are the property of Life Technologies Corporation or their
respective owners
LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL
WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED,
INCLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY, FITNESS FOR
A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED
BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S)
BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY
STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT,
PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH
OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE
USE THEREOF.
For support visit www.lifetechnologies.com/support or
email [email protected]
www.lifetechnologies.com
Gateway® pCMV•SPORT6, Not I-Sal I Cut
Cat. no.
12209-011
Size
5 μg
Pub. Part no. 12209011.pps
MAN0001048
Conc. 50 μg/mL
Store at −30°C to −20°C
Rev. Date 27 January 2012
Description
The Gateway® pCMV•SPORT6 Not I-Sal I Cut vector is designed for
directional cloning of cDNA that contain Not I and Sal I-compatible termini.
The vector contains attB sites so that the clones from the resulting cDNA
library can be used in conjunction with the Gateway® Cloning Technology
(Hartley, 2000). The Gateway® Cloning Technology is a novel universal
system for cloning and subcloning DNA segments, facilitating gene functional
analysis, and protein expression. The Gateway® BP Reaction is based on the
site-specific recombination system of bacteriophage lambda, and can be
represented as follows:
attB1-gene-attB2 × attP1-ccdB-attP2 ⇒ attL1-gene-attL2 × attR1-ccdB-attR2
(cDNA in pCMV•SPORT6) (Donor Vector)
(Entry Clone)
(by-product)
Important Features
• Gateway® pCMV•SPORT6 Vector has been digested with Sal I (5′) and Not I
(3′) for construction of cDNA libraries containing Not I and Sal I termini.
cDNA (containing Kozak and ATG) cloned into this vector can be
expressed in mammalian cells.
• Gateway® pCMV•SPORT6 Vector contains a CMV promoter upstream of
the attB1 site followed by restriction endonuclease sites and attB2.
Downstream of the attB2 sequence is the SV40 small t-intron and
polyadenylation signal.
• This vector contains the SP6 and T7 RNA polymerase promoters flanking
the attB1 and attB2 sites, respectively. The cDNA can be sequenced from
either direction using suitable primers. The vector contains a pUC origin of
replication, an F1 intergenic region, and an ampicillin resistance marker.
Intended Use: For research use only.
Not intended for any animal or human therapeutic or diagnostic use.
3
2
NgoA IV 4265
Dra III 4159
Important Features, cont.
• cDNA cloned into this vector can be transformed into ElectroMAX™
DH10B™ cells and the library can be plated and screened (Sambrook, 1989)
amplified (Kriegler, 1990), used for the production of single-stranded
plasmid DNA in vivo (Li, 1994), or for preparation of double-stranded
plasmid as a substrate for the Gene Trapper® cDNA Positive Selection
System (Cat. No. 10356-020).
• cDNA cloned into Gateway™ pCMV•SPORT6 can be transferred into a
Donor Vector (e.g., pDONR™201) using Gateway™ BP Clonase® Enzyme
Mix.
Cla I 122 Hpa I 257
Mun I 268
Mam I 356
Bcl I 361
Pfl M I 3846
Nhe I 637
f1 intergenic
region
SV40
polyadenylation signal
incA
T7 promoter
attB2
attB1
SP6 promoter
Xmn I 3460
Bcg I 3381
Sca I 3343
multiple
cloning
site
Kpn I 822
Stu I 872
Sst II 918
Avr II 924
Bbs I 960
Esp3 I 992
Mlu I 736
pCMV•SPORT 6
Apr
4396 bp
CMV promoter
References
Nco I 1227
SnaB I 1249
Hartley, J.F., Temple, G.F., and Brasch, M.A. (2000) Genome Res. 10, 1788.
Kriegler, M. (1990) Gene Transfer and Expression: A Laboratory Manual. Stockton
Press, New York, NY.
Bsa I 2932
lox P
Eam1105 I 2860
Nsi I 1534
ori
Li, W.-B., Gruber, C.E., Lin, J.-J., Lim, R., D'Alessio, J.M., and Jessee, J.A. (1994)
BioTechniques 16, 722.
Sap I 1807
BspLU11 I 1930
Alw N I 2341
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A
Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory, Cold Spring
Harbor, NY.
Map of Gateway™ pCMV•SPORT6, Not I-Sal I Cut.
The cDNA is cloned between the Not I and Sal I sites of the multiple cloning
site.
The sequence has not been confirmed by sequence analysis. It was assembled
from the known sequence of fragments used to construct the vector.
Vector sequences, restriction information, and maps can be found in the
Vector Data area of our web site, www.lifetechnologies.com/support.
3
2
NgoA IV 4265
Dra III 4159
Important Features, cont.
• cDNA cloned into this vector can be transformed into ElectroMAX™
DH10B™ cells and the library can be plated and screened (Sambrook, 1989)
amplified (Kriegler, 1990), used for the production of single-stranded
plasmid DNA in vivo (Li, 1994), or for preparation of double-stranded
plasmid as a substrate for the Gene Trapper® cDNA Positive Selection
System (Cat. No. 10356-020).
• cDNA cloned into Gateway™ pCMV•SPORT6 can be transferred into a
Donor Vector (e.g., pDONR™201) using Gateway™ BP Clonase® Enzyme
Mix.
Cla I 122 Hpa I 257
Mun I 268
Mam I 356
Bcl I 361
Pfl M I 3846
Nhe I 637
f1 intergenic
region
SV40
polyadenylation signal
incA
Xmn I 3460
Bcg I 3381
Sca I 3343
multiple
cloning
site
Kpn I 822
Stu I 872
Sst II 918
Avr II 924
Bbs I 960
Esp3 I 992
Mlu I 736
T7 promoter
attB2
attB1
SP6 promoter
pCMV•SPORT 6
Apr
4396 bp
CMV promoter
References
Nco I 1227
SnaB I 1249
Hartley, J.F., Temple, G.F., and Brasch, M.A. (2000) Genome Res. 10, 1788.
Kriegler, M. (1990) Gene Transfer and Expression: A Laboratory Manual. Stockton
Press, New York, NY.
Bsa I 2932
lox P
Eam1105 I 2860
ori
Li, W.-B., Gruber, C.E., Lin, J.-J., Lim, R., D'Alessio, J.M., and Jessee, J.A. (1994)
BioTechniques 16, 722.
Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A
Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory, Cold Spring
Harbor, NY.
Nsi I 1534
Sap I 1807
BspLU11 I 1930
Alw N I 2341
Map of Gateway™ pCMV•SPORT6, Not I-Sal I Cut.
The cDNA is cloned between the Not I and Sal I sites of the multiple cloning
site.
The sequence has not been confirmed by sequence analysis. It was assembled
from the known sequence of fragments used to construct the vector.
Vector sequences, restriction information, and maps can be found in the
Vector Data area of our web site, www.lifetechnologies.com/support.