MOLECULAR TECHNIQUES FOR THE IDENTIFICATION OF COMMENSAL FUNGAL
POPULATIONS ON CAVE ROOSTING BATS
A Thesis
Presented to
The Graduate Faculty of The University of Akron
In Partial Fulfillment
of the Requirements for the Degree
Master of Science
Kelsey Njus
August, 2014
i
MOLECULAR TECHNIQUES FOR THE IDENTIFICATION OF COMMENSAL FUNGAL
POPULATIONS ON CAVE ROOSTING BATS
Kelsey Njus
Thesis
Approved:
Accepted:
____________________________________________
Advisor
Dr. Hazel A. Barton
____________________________________________
Dean of the College
Dr. Chand Midha
____________________________________________
Committee Member
Dr. James Holda
____________________________________________
Dean of the Graduate School
Dr. George R. Newkome
____________________________________________
Committee Member
Dr. Greg Smith
____________________________________________
Date
____________________________________________
Department Chair
Dr. Monte Turner
ii
ABSTRACT
Since the emergence of White-nose Syndrome (WNS) in 2006, North American
bat populations have experienced devastating population declines. WNS is caused by the
psychrophilic fungus Pseudogymnoascus destructans (Pd), which invades the wing
tissues of bats during torpor, resulting in up to a 97% mortality rate. Yet some species,
including the Indiana bat (Myotis sodalis) and the Virginia big-eared bat (Corynorhinus
townsendii virginianus), appear to have reduced mortality from WNS, despite close
proximity to infected bats. In an attempt to determine whether bats possess commensal
fungal populations that confer resistance to WNS, we analyzed the culturable and nonculturable fungal communities of bat fur. To generate a library of the non-culturable
fungal species, DNA was isolated from bat fur clippings, amplified by PCR of the
internal transcribed spacer (ITS) region, sequenced, and identified using BLAST.
Culturable fungal populations were obtained by swabbing and isolation in pure culture,
and also identified using the ITS sequence. By comparing the communities of bats
belonging to five species for four locations, we were able to determine that the species of
the bat is the most important contributor to the composition of a bat’s fungal community,
and this was best analyzed using molecular methods. While the majority of shared fungal
species consisted of saprotrophic fungi, a small portion of these shared species consisted
of yeast, the most abundant of which was Debaryomyces udenii; Virginia big-eared bats,
which have a 0% mortality rate, have a fungal community that consists of 55% D. udenii.
iii
This data supports the hypothesis that bats harbor commensal fungal communities, and
that these communities may confer resistance to WNS. Identifying whether commensal
populations provide a mechanism of WNS resistance in bats could help in our
understanding of the emergence of this pathogen, the future of the disease, and potential
biological controls.
iv
ACKNOWLEGEMENTS
The authors would like to thank Greg Turner (Pennsylvania Game Commision),
Brooke Hines (Kentucky Department of Fish and Wildlife Resources) and Craig
Stihler (West Virginia Division of Natural Resources) for bat handling and sampling
assistance, Virginia and Elizabeth Shelley and Samantha Kaiser for culturing
assistance, and the technicians at Advanced Genetics Technologies Center
(University of Kentucky) for their assistance in sample analyses. Lastly, we would
like to thank Bat Conservation International (Grant # 535322) for funding our
research.
v
TABLE OF CONTENTS
Page
LIST OF TABLES …………………………………………………………………………………….…..….…. vii
LIST OF FIGURES …………………………………………………………………………….………….…… viii
CHAPTER
I. INTRODUCTION ……………………………………………………………………………………………... 1
White-nose Syndrome and Commensalism in Bats .………….………………………. 1
II. MATERIALS AND METHODS ………….………………………………………………………………. 6
Sample Sites .……..…….…………………………………………………………………...…..……... 6
Fur and Swab Collection ………………………………………….…….…………………….…… 7
Molecular Identification of Fungal Isolates ………..….………………………………… 10
Culture Identification of Fungal Isolates ……………………………….………...………. 13
III. RESULTS ……………………………………………………….…………………………………………. 14
Molecular versus Culture Identification of Fungal OTUs …..………………………… 14
Molecular Analysis of Fungal Populations on Bat Pelage ……………………...…….. 19
Generation of Fungal Profiles ……………….…………………………………………….……. 23
IV. DISCUSSION ………………….………………………..………………………………………………….. 37
V. CONCLUSION ……………………………………………………………………………………………….. 48
LITERATURE CITED ……………...……………………..…………………………………………….......... 49
APPENDIX: TABLES ….…………………………...……...…………….….………………..………….…… 53
vi
LIST OF TABLES
Table
Page
2.1
Summary of bats sampled at Canoe Creek State Park. …………………………………… 8
2.2
Summary of bats sampled at Casselman River Mine …………………….….…………… 8
2.3
Summary of bats sampled at Minor Rexrode Cave ………….……….…….……......…… 9
2.4
Summary of bats sampled at Schoolhouse Cave …………………………….………..…… 9
3.1
Summary of Pseudogymnoascus species and Pd isolated from bats using
molecular and culture techniques ……………...………………………………….…………… 16
3.2
Techniques and Chemicals used to overcome PCR inhibition ….……..………… 23
3.3
Summary of all samples processed successfully for molecular analysis …….....… 25
3.4
Distribution of fungal isolates from five species of bats and four locations …….. 34
A1
All fungal OTUs identified for each bat …………………………………………...………. 53
vii
LIST OF FIGURES
Figure
Page
3.1
Fungal species identified on little brown bats using molecular ID methods
versus culture ID methods ……………………………………………………………………...… 18
3.2
Fungal species identified on Indiana bats using molecular ID methods
versus culture ID methods…………………….……………………………………………..…… 19
3.3
Sample PCR inhibition gel..…………………………………………………………...…………. 21
3.4
Fungal community of each bat sampled …………………………………...……...………… 26
3.5
A comparison of the fungal OTUs identified on bats from Canoe Creek State
Park (CCSP) ……………………………………………………..…………………………...………. 29
3.6
A comparison of the fungal OTUs identified on bats from Casselman River
Mine (CRM) ……………………………………………………..…………………………...………. 30
3.7
A comparison of the fungal OTUs identified on bats from Minor Rexrode
Cave (MRC) ……………………………………………………...…………………………...………. 30
3.8
A comparison of the fungal OTUs identified on bats from Schoolhouse Cave
(SHC) …………..…………………………………………………..…………………………...………. 31
3.9
A comparison of the fungal OTUs identified on all little brown bats
(LBs) sampled ……………………………………………….………...……….…..………………… 31
3.10
A comparison of the fungal OTUs identified on all big brown bats
(BBs) sampled ………………………………………………………...……….…..………………… 32
3.11
A comparison of the fungal OTUs identified on all Virginia big-eared bats
(COTOs) sampled ……………………….…………………………...……….…..………………… 32
3.12
A cluster dendrogram based on Bray-Curtis dissimilarities between
individual bat communities based on the presence or absence of different
fungal species ……………………………………………………………………………………….... 36
viii
4.1
The fungal communities of all bat species, presented with yeast species in
color (filamentous fungi in greyscale) and the mortality rates of each bat
species presented above the bars ………………….……………………………...………….… 45
4.2
A colony of Virginia big-eared bats in Schoolhouse Cave, West Virginia ……… 46
4.3
A sterile cotton swab used to sample the oily, yellow substance on the fur of
the Virginia big-eared bats in Figure 4.2.…………….………………………..………..…… 47
ix
CHAPTER I
INTRODUCTION
White-nose Syndrome and Commensalism in Bats
Humans are ubiquitously populated by commensal microorganisms living on our
bodies, which are comprised of bacterial, archeal, and fungal populations.
Commensalism, which differs from mutualism because it is non-obligitory, is defined as
a relationship in which the commensal benefits, while the host is unharmed (Blaser 1997).
This relationship is often beneficial to both the host and the commensal; the host provides
the commensal the nutrients and habitat it needs to survive, while the commensal
prevents the establishment and growth of pathogenic microorgansms. This protection by
the commensal works in a variety of ways: 1) stimulation of the host’s innate immune
response (Casadevall and Pirofski, 2000); 2) prevention of the establishment of
pathogenic microorganisms via resource acquisition and competitive exclusion
(Casadevall and Pirofski, 2000); 3) secretion of antimicrobial peptides which kill or
inhibit the growth of harmful microorganisms; and 4) production of metabolic byproducts
that alter their environment (e.g. lower the pH), thereby making it inhospitable to
potential pathogens (Grice and Segre, 2011). While it is likely that animals play host to
similar protective microorganisms, the commensal populations that protect them in the
wild remain unknown. Recently, a fungal epidemic known as White-nose Syndrome has
increased interest in commensalism in bats.
1
White-nose syndrome (WNS) is caused by the fungus Pseudogynmoascus
destructans (Pd), formerly known as Geomyces destructans (Minnis and Lindner 2013).
This cutaneous fungal disease, which affects hibernating cave bats (Lorch et al., 2011), is
characterized by a powdery white fungal growth on the muzzles, ears, and wing
membranes of infected bats (Blehert et al., 2009). Evidence of WNS was first
documented in in Howe’s Cavern, New York in 2006 (Blehert et al., 2009). Since then
the epidemic has spread across the eastern United States and into Canada, killing more
than five million North American bats (Meteyer et al., 2012). Of the bat species within
the WNS endemic area, seven are currently susceptible to infection by WNS. These
include the big brown bat (Eptesicus fuscus), the Eastern small-footed bat (Myotis leibii),
the gray bat (Myotis grisescens), the Indiana bat (Myotis sodalis), the little brown bat
(Myotis lucifugus), the Eastern tri-colored bat (Perimyotis subflavus), and the northern
long-eared bat (Myotis septentrionalis) (Turner et al., 2011). White-nose Syndrome is
the first documented case of an invasive fungal epidemic in endothermic organisms,
although bats are functionally exothermic during their long periods of torpor (Cryan et al.,
2010). Torpor is a metabolically depressed state used to conserve energy during periods
of scarce food supply (Bouma et al. 2010) that may last days to weeks (Geiser & Ruf,
1995). During torpor, metabolic rates can decrease to less than 5% of basal rates with a
corresponding decrease in body temperature (Tb) to near ambient temperatures. In bats,
Tb is approximately 2-8oC during torpor (Reeder et al. 2012), while Pd has an optimal
growth at ~10oC.
Several important factors have been proposed which may affect the severity of Pd
infection, including hibernacula conditions (Grieneisen, 2011), acquired immune
2
responses (Bouma et al., 2010), and body mass (Reeder et al., 2012); however, certain
pathologies have been observed regardless of these conditions. Previous studies have
demonstrated that bats infected by Pd have an increased frequency of arousal from torpor
and a higher prevalence of emaciation (Reeder et al. 2012). Initially it was assumed that
more frequent arousals cause an increase in stored fat consumption during hibernation
and that death results from starvation (Blehert et al., 2009; Boyles et al., 2009; Jonasson
and Willis, 2011; Reichard et al., 2009), although recent studies show that body mass
index (BMI) is not related to survival in WNS affected bats. This indicates that some
other physiological impairment is causing mortality (Reeder et al., 2012), but the
frequency of arousals does appear to be a significant predictor of mortality. Wing
membranes in bats are critical for gas exchange and maintenance of water balance and it
has been suggested that Pd invasion may damage the wing membrane to the point that
osmotic balance and homeostasis can no longer be maintained during torpor. This would
result in an increased frequency of arousal from torpor due to dehydration (Willis et al.,
2011; Cryan et al. 2010), and water seeking behavior. Both would account for the
observation that infected bats tend to fly outside of hibernacula during winter months.
Nevertheless, WNS has not caused equal destruction among all the species it
affects. M. sodalis is an endangered species found throughout the eastern United States
(Kurta & Whitaker, 1998). Although M. sodalis has been affected by WNS, it has
experienced reduced mortality due to WNS (mortality rate = 72%) compared to other
species, such as M. lucifigus (mortality rate = 91%) (Turner et al., 2011). The
endangered C. townsendii virginianus is distributed across the Appalachian Mountains
(Johnson et al., 2005), yet no WNS associated mortality has been observed in this species
3
despite hibernation in caves where other species have experienced mass mortalities
(Craig Stilher, personal communication, 2011). Additionally, although Pd has been
positively confirmed on this species via PCR, no WNS pathology has been observed.
Because of these observed discrepancies in mortality rates we endeavored to discover
whether M. sodalis and C. townsendii may have reduced mortalities from WNS due to
species specific protective commensal populations.
Although bat commensal populations are unknown, studies have shown that bats
play host to a multitude of microorganisms, including fungal (Larcher et al., 2003;
Wibbelt et al., 2010) and bacterial (personal observation) species. Unfortunately, the data
regarding the commensal microorganisms present on bats, including their normal flora, is
greatly limited compared to what is understood regarding human commensals. For
example, it is unclear what populations of nonpathogenic fungal species are normally
found on bats. Understanding whether members of Pseudogymnoascus commensals of
bats may also shed some light on the origin of Pd; finding few members of this genus
would support the currently favored novel species hypothesis, which assumes that Pd was
only recently introduced into the United States from Europe (Minnis and Lindner, 2013).
It is also unknown whether the presence of certain fungal species on bats is correlated
with the location of the bat, the species of the bat, or both. Understanding what predicts
the incidence of fungal species could provide insight into whether bats maintain a
commensal fungal population or whether they simply act as passive vectors for fungi
present in their habitats. Finally, the current methodology for microbial identification on
bats is via cultivation, rather than molecular techniques, yet 99.9% of microbes found in
nature cannot be grown in culture (Amann et al. 1995).
4
In this study we address two central questions: (1) What is the most accurate and
sensitive method for the identification of fungi present on bat fur, and (2) do these fungi
represent commensal populations which could confer resistance to WNS? In order to
analyze what role commensals may play in resistance we developed a sensitive method
for analyzing the fungal communities present on bat fur and used it to generate fungal
profiles of bat species with differing mortality rates from WNS. Distinct differences in
fungal populations between different bat species and the presence of specific fungal
species suggests that commensal populations can provide resistance to Pd infection.
5
CHAPTER II
MATERIALS AND METHODS
Sample Sites
Swab samples from bats were collected for culture analysis from Cuijo’s (CUGA)
and Salt Petre (SPC) caves, Virginia, USA under the auspices of the Kentucky and
Virginia Departments of Natural Resources. Both swab samples and clippings of bat for
molecular analysis were collected from caves in Canoe Creek State Park (CCSP),
Pennsylvania, USA; Casselman River Mine, Pennsylvania, USA; Minor Rexrode Cave,
West Virginia, USA; and Schoolhouse Cave, West Virginia, USA under local and
Federal collection protocols. Samples were collected from Canoe Creek State Park on
October 11, 2012 during fall swarming while bats were still active in environments
outside of caves; Casselman River Mine, Minor Rexrode Cave, and Schoolhouse Cave
samples were collected during winter surveys of bat hibernacula on February 7, 2012;
February 12, 2012; and February 21, 2013 respectively.
Canoe Creek State Park is rich in limestone which was mined throughout the
early 1900s, creating the abandoned mines used by bats as hibernacula today. From the
late 1800’s to the early 1900’s the area along the Casselman River between Boynton and
Meyersdale, PA was mined primarily for coal. Today, only a portion of the mine remains
active, and all sampling was conducted in inactive areas of the mine. Minor Rexrode
Cave is developed in Helderberg Group limestone’s under the northewest limb of the
6
Neds Mountain Anticline in Pendleton County, PA, and is one of the few Helderberg
caves with a stream. Schoolhouse Cave is formed in horizontal Black River Group
limestones west of the axis of the Wills Mountain Anticline in Germany Valley,
Pendleton County, PA. Schoolhouse Cave is a mostly dry segment of an extensive karst
hydrologic system which underlies the eastern side of Germany Valley.
Fur and Swab Collection
In order to determine the most sensitive sampling method for this study, we
decided to compare the fungal isolates identifiable by cultivation with the operational
taxonomic units (OTUs) identifiable by molecular techniques. An operational taxonomic
unit is the standard designation for a fungal species identified without isolation in pure
culture. For molecular analysis, bat fur was collected from the following species: Myotis
lucifugus, Myotis sodais, Myotis leibii, Eptesicus fuscus, Corynorhinus townsendii, and
Perimyotis subflavis. These species were chosen for their overlapping geographical
range and varying susceptibility to Pd. Tables 2.1 – 2.4 summarize the bats sampled for
this study for each site sampled, as well as whether Pseudogymnoascus was identified by
cultivation and if a molecular profile was generated. Approximately 10 mg of fur was
collected with sterile scissors from between the shoulder blades of bats and stored in γirradiated vials for DNA extraction. For cultivation, bats were swabbed with moistened
cotton tipped applicators for cultures along the forearm and around the muzzle and ears.
Swabs were used to inoculate potato dextrose agar (PDA) plates consisting of 4g potato
extract, 20g dextrose, and 15g agar per liter of water (pH 6.8 - 7.0). See Tables 2.1 - 2.4
for a summary of samples collected by site, species, and gender.
7
Table 2.1. Summary of bats sampled at Canoe Creek State Park on November 11, 2012.
LB = little brown (bat); BB = big brown (bat); IND/MYSO = Indiana bat. M = male; F =
female. Pseudogymnoascus in Culture indicates whether Pseudogymnoascus was found
in culture: + = Pseudogymnoasucs found in culture; - = Pseudogymnoascus not fond in
culture; NA = culture sample not taken. For Fungal Profile: Y = fungal profile
successfully generated via molecular techniques; NA = fur clipping not taken; N =
generation of fungal profile failed.
Bat
Sex
Pseudogynmoascus In Culture
Fungal Profile
LB1
M
+
N
LB2
M
-
N
LB3
F
+
Y
LB4
M
-
NA
LB5
M
-
Y
LB6
F
+
N
LB7
M
NA
NA
LB8
M
NA
Y
IND1
NA
+
N
BB1
F
-
Y
Table 2.2 Summary of bats sampled from Casselman River Mine on February 7, 2012.
LB = little brown (bat); BB = big brown (bat); ESF = Eastern small-footed (bat); PIP =
Tricolored bat. M = male; F = female. Pseudogymnoascus in Culture indicates whether
Pseudogymnoascus was found in culture: + = Pseudogmnoascus found in culture; - =
Pseudogynmoascus not fond in culture. For Fungal Profile: Y = fungal profile
successfully generated via molecular techniques; N = generation of fungal profile failed.
Bat
Sex
Pseudogymnoascus In Culture
Fungal Profile
BB2
F
+
N
BB3
M
-
N
BB4
M
+
Y
BB5
F
+
Y
BB6
F
-
Y
BB7
M
-
Y
LB9
F
-
Y
LB10
M
+
Y
LB11
M
-
Y
ESF1
F
-
Y
ESF2
M
-
Y
PIP1
M
+
N
8
Table 2.3 Summary of bats sampled from Minor Rexrode Cave on February 21, 2013.
MYSO = Indiana bat; COTO = Virginia big-eared bat. M = male; F = female.
Pseudogymnoascus in Culture indicates whether Pseudogymnoascus was found in
culture: + = Pseudogymnoascus found in culture; - = Pseudogymnoascus not found in
culture. For Fungal Profile: Y = fungal profile successfully generated via molecular
techniques; N = generation of fungal profile failed.
Bat
Sex
Pseudogynmoascus In Culture
Fungal Profile
MYSO1
M
+
Y
MYSO2
M
-
Y
MYSO3
M
-
Y
COTO4
M
-
N
COTO5
M
-
N
COTO6
M
-
Y
COTO7
F
+
Y
COTO8
F
-
Y
COTO9
F
-
Y
Table 2.4 Summary of bats sampled from Schoolhouse Cave on February 28, 2013.
COTO = Virginia big-eared bat. M = male; F = female. Pseudogymnoascus in Culture
indicates whether Pseudogymnoascus was found in culture: X = swab not taken for
culture inoculation; - = Pseudogymnoascus not found in culture. For Fungal Profile: Y =
fungal profile successfully generated via molecular techniques; N = generation of fungal
profile failed; NA = fur clippings not taken for the generation of a fungal profile via
molecular techniques.
Bat
Sex
Pseudogymnoascus In Culture
Fungal Profile
COTO1S
F
X
NA
COTO2S
F
X
NA
COTO3S
F
X
NA
COTO4S
F
X
NA
COTO5S
M
X
NA
COTO6S
M
X
NA
COTO7
M
-
Y
COTO8
M
-
Y
COTO9
M
-
Y
COTO10
M
-
Y
COTO11
F
-
Y
COTO12
F
-
Y
9
Molecular Identification of Fungal Isolates
Total DNA was isolated from bat fur using the YeaStar genomic DNA kit (Zymo,
Irvine, CA) according to the manufacturer’s instructions using the chloroform-free
protocol (Protocol II) and eluted in 6-40 µl dH2O. Later, 10 µl of Bovine Serum
Albumin (BSA) was added to the extraction mixture just prior to the incubation step in
order to relieve interference from inhibitors. Fungal DNA was amplified using PCR with
fungal-specific primers for the internal transcribed spacer (ITS) 18S gene sequence. PCR
was initially performed using forward primer ITS5 (5’ – GGAAGTAAAAGTCGTAAC
AAGG – 3’) and reverse primer ITS4 (5’ – TCCTCCGCTTATTGATATGC – 3’). The
reaction mix contained 1-20 ng/µl template, 10 μl Taq 2X master mix (New England
Biolabs, Ipswich, MA), and 300 mM each of the forward and reverse primers, in a final
volume to 20 µl. The initial thermal cycling parameters were a hot start at 94oC for 5
minutes, followed by 36 cycles of 94oC for 45 seconds, annealing at 60oC for 1 minute,
and 72oC for 1 minute 30 seconds. The results of the PCR were visualized by running 25 µl of the PCR product and 3 µl 10X loading dye on a 1.2% agarose gel containing 10%
Ethidium Bromide. Samples were run for approximately 20 minutes at 75 mAMP and
120 V in 1X LB buffer, after which they were placed in a UV light box for examination.
In some cases the initial PCR was successful and the product was used for cloning and
OTU identification; however, for a number of samples, particularly those collected from
Casselman River Mine, this initial PCR was unsuccessful.
In reactions that failed, presumably due to contaminants, we examined the
extracted DNA using a poisoning control. To do this, sample DNA was mixed with 50
ng of mixed environmental DNA obtained from a cave and known to amplify using
10
fungal specific primers (personal observation). If the PCR amplification of the mixed
sample failed, it indicated that substances present in the fungal DNA prevented the
amplification of environmental control, confirming the presence of PCR inhibitors.
If amplification was not adequate for visualization of the PCR product on the
agarose gel due to the presence of PCR inhibitors, nested PCR was performed on
inhibited samples using an external pair of primers to amplify the template, followed with
a second round of PCR using internal primers. The first round of amplification was
carried out using the external forward primer NSA3 (5’-AAACTCTGTCGTGCTGG
GGATA – 3’) and external reverse primer ITS4-B (5’ –CAGGAGACTTG TACAC
GGTCCAG – 3’) using 1 µl of DNA template, 10 μl Taq 2X master mix, 300 mM
NSI1and ITS4-B, and dH2O to a final volume to 20 µl. The second-round PCR reaction
was performed using internal forward primer ITS5 and reverse primer ITS4 using 1 µl of
first round PCR product as the template as described. The thermal cycling parameters for
the first reaction were identical to the initial thermal cycling parameters except an
annealing temperature of 56oC was used. Second round thermal cycling parameters were
identical to the initial thermal cycling parameters.
In samples that could not be amplified using the nested protocol we tried to
extract the inhibitors via EDTA/EGTA using a Thermo Mini Dialysis Unit (Slide-ALyzer) which was dialyzd against 100mM EDTA/EGTA at 4oC overnight with stirring.
Samples that continued to fail to reach sufficient amplification were ethanol purified in
two volumes of 100% ethanol and 1/10th final volume of 3M NaOAc. Samples were
then incubated at -20oC for one hour, after which they were centrifuged at 3500xg for 20
minutes, washed twice with 70% ethanol, and allowed to dry. These samples were re-
11
suspended in 10μL of water and stored at -20oC until needed. We also tried manipulating
the PCR reaction in order to increase product specificity and yield. This was done by
adding extra 1.5μL MgCl2 per reaction to the nested PCR along with 4 μL of betaine.
Additionally, we tried using TaqGold (Life Technologies, Grand Island, NY) in place of
Taq 2X master mix (New England Biolabs, Ipswich, MA).
This also failed, so we tried using AccuPrime Taq Polymerase in an
MgSO4/EDTA buffer (Invitrogen, Grand Island, NY) along with the addition of 10 μL of
BSA to the lysis mixture as specified by the Yeastar genomic DNA kit (Zymo, Irvine,
CA). For nested PCR using this method, the first round of amplification was performed
using 1-20 ng/μL template, 5 μL 10X Buffer II, 300 mM NSA3 and 300 mM ITS4-B, 0.5
μL AccuPrime Taq, and dH2O to a final volume to 50µL. The thermocycling parameters
included a hot start at 94oC for 1 minute, followed by 35 cycles of 94oC for 30 seconds,
annealing at 60oC for 30 seconds, and 68oC for 1 minute. The reaction mixture used in
the second round of PCR was identical except that 1μL of the first round PCR product
was used as template along with 10X Buffer I, and ITS4 and ITS5 as primers. The
thermocycling parameters were identical except that the annealing temperature was 60oC.
Finally, extracted DNA samples were eluted from the Yeastar genomic DNA kit spin
columns (Zymo, Irvine, CA) in larger volumes of water (≥ 20 μL as opposed to 6-10 μL).
Using this method, we successfully amplified the fungal samples for which we had
remaining DNA. All PCR reactions were run with contamination controls that contained
nuclease free water; the appearance of a PCR product in this reaction indicated
contamination, and the amplifications were discarded.
12
All successfully amplified PCR products were purified using a Genomic DNA
Clean & Concentrator kit (Zymo, Irvine, CA), eluted in 6 µl dH2O, and cloned using the
TA Cloning Kit (Invitrogen, Grand Island, NY) following the manufacturers’ protocols.
Clones were sent to Advanced Genetics Technology Center (AGTC)
(http://www.uky.edu/Centers/AGTC/) for sequencing. Obtained equences were aligned
using the multi-sequence alignment function in Geneious Pro (Biomatters Ltd., Auckland,
New Zealand). Consensus sequences were generated and compared against all sequences
available in Genbank (NCBI) using the BLAST algorithm (Johnson et al., 2003). The
fungal profiles generated for each bat were analyzed using R (Institute for Statistics and
Mathematics, Aktuellers der WU (Wirtschaftsuniversitat Wien), Vienna, Austria.
Culture Identification of Fungal Isolates
Culture plates were analyzed for the presence of Pseudogymnoascus species after
~1 week of growth at 10oC using colony morphology and microscopy. After staining with
lactophenol cotton blue, positive identification of a Pseudogymnoascus species consisted
of visualization of characteristic branched conidiophores with individual spores or chains
of spores (~2µm in diameter) attached directly to the branches and branch tips. Samples
positively identified were maintained at 10oC as part of a fungal culture library. For
species identification, the genomic DNA was extracted using a Fungal/Bacterial DNA kit
(Zymo, Irvine, CA) following the manufacturer’s recommended protocol. The ITS
sequences were than PCR amplified, sequenced, and identified using BLAST as
described.
13
CHAPTER III
RESULTS
Molecular versus Culture Identification of Fungal OTUs
In order to determine the most sensitive sampling method for this study, we
compared the fungal ITS sequences identifiable from bat fur by cultivation versus
molecular techniques. To analyze culturable and nonculturable fungal communities on
bats, swab and fur samples were collected from six bat species; Myotis lucifugus (little
brown bat), Myotis sodais (Indiana bat), Myotis leibii (Eastern small-footed bat),
Eptesicus fuscus (big brown bat), Corynorhinus townsendii virginianus (Virginia bigeared bat), and Perimyotis subflavis (Eastern tri-colored bat). These species were chosen
as their regional distribution overlaps that of Pd, while each species demonstraties
varying susceptibility and mortality from WNS. This allowed us to compare the fungal
communities of susceptible and non-susceptible bat species hibernating within regions of
potential Pd exposure and determine whether any similarities between their fungal
communities exist.
We compared the culture versus molecular approaches in two ways: 1) we
examined whether Pd could be identified from an individual bat that had been sampled
for culture and molecular analysis; and 2) we examined the total fungal community
identified via cultivation versus molecular methods to determine whether molecular
methods could identify unique populations of fastidious fungi that were not found by
14
culture alone. Each bat was sampled for molecular and culture analysis by fur clipping
and swabbing respectively; the swabs were used to inoculate plates where isolates were
subcultured via the pure culture technique. Any presumptive Pseudogymnoascus isolates
were identified via microscopy and confirmed via DNA isolation and ITS sequencing.
The identities of isolates were then compared to the OTU fungal profile of that bat
generated via molecular methods.
Pseudogymnoascus was isolated from cultures grown from swabs taken from two
little brown bats (CCSP LB1 and CCSP LB6) and one Indiana bat (CCSP IND1) at
Canoe Creek State Park (CCSP); three big brown bats (CRM BB2, CRM BB4, and CRM
BB6), one little brown bat (CRM LB10), and one Eastern tri-colored bat (CRM PIP1)
from Casselman River Mine; and one Indiana bat (MRC MYSO 1), and two Virginia bigeared bats (MRC COTO6 and MRC COTO7) from Minor Rexrode Cave (MRC).
Pseudogymnoascus species were also identified on each of these bats using molecular
methods alone; a second Pseudogymnoascus species was also identified on a Virginia
big-eared bat (MRC COTO7) from Minor Rexrode Cave via molecular techniques, but
not via cultivation. Table 3.1 summarizes the culture and molecular data for each bat
from which a Pseudogymnoascus species was identified, as well as the closest relative
identified on NCBI via comparison of ITS sequences in BLAST, and the percent of
identical base pairs of the sequence and its closest relative (percent identity).
15
Table 3.1 Summary of Pseudogymnoascus species and Pd isolated from bats using
molecular and culture techniques. Accession # refers to the NCBI accession number
associated with the top BLAST match for each sequence. An * indicates that for that bat,
the closest ID’d relative found using culture methods and the closest ID’d relative found
using molecular methods had 98% sequence homology, indicating that they are the same
species despite having different accession numbers. NA indicates that Pd or a
Pseudogynmoascus species was not identified for that bat using the indicated method.
Pseudogymnoascus species and Pd isolated from Bats using Molecular and Culture
Techniques
Culture Data
Molecular Data
Location
Bat
Closest ID'd Relative
% Ident.
Accession #
Closest ID'd Relative
% Ident
Accession #
CRM
BB4
Geomyces sp.*
99%
JX270438.1
Geomyces sp.*
100%
JX270501.1
CRM
BB6
Geomyces sp.*
99%
JF439475.1
Geomyces sp. *
100%
JX270501.1
CRM
LB10
Geomyces destructans*
99%
EU884920.1
Geomyces destructans*
99%
HE588133.1
MRC
MYSO1
Geomyces sp.*
99%
JX270555.1
Geomyces sp.*
99%
JX270501.1
MRC
COTO6
Geomyces sp.
100%
KF212291.1
Gymnoascus sp.
99%
JX270593.1
MRC
COTO6
Geomyces sp.*
100%
KF212291.2
Geomyces destructans*
99%
HE588133.1
MRC
COTO7
Geomyces destructans*
100%
HF911371.1
Geomyces destructans*
99%
HE588133.1
MRC
COTO7
NA
Geomyces sp.
99%
KF212294.1
*Greater than or equal to 98% sequence homology = Same species
The observed differences in the detection of Pseudogymnoascus species suggest
that molecular techniques were equally or more sensitive than culture techniques to
identify Pseudogymnoascus species on these bats. We therefore decided to utilize culture
data collected from a previous study of little brown and Indiana bats in order to determine
whether molecular techniques were also sufficient to determine total fungal populations
(Kaiser and Barton, unpublished). This study generated culture libraries from these
animals in an attempt to identify all fungal species via cultiviation from four little brown
bats and five Indiana bats located across a variety of cave locations. The fungal isolates
identified from these bats were compared to the molecular profiles generated for five
little brown bats and three Indiana bats in this study. These results (Figures 3.1 and 3.2)
revealed that a far greater number and variety of fungal genera could be identified using
16
molecular methods. On little brown bats, 51 genera of fungi were identified using
molecular methods, while only five genera were identified via culture. Three of the five
genera identified by cultivation were also identified using molecular methods alone; of
the two genera not identified via molecular methods, one was found to be an unidentified
fungal clone by its ITS sequence in the NCBI database, and the other was a member of
the genus Isaria. Twenty-two genera of fungi were identified via molecular techniques
on Indiana bats, while only 6 were identified in culture. Four of the six cultured isolates
were also identified via molecular methods; the two not identified were members of the
genera Zygorhynchus and Mucor. These results indicate that molecular methods are
superior to cultivation for the purpose of surveying fungal communities. We therefore
decided to use only molecular techniques to identify the commensal populations present
on bat fur.
17
Fungi Identified on Little Brown Bats
100%
51 genera
5 genera
Isaria
80%
Unculturedfungusclone
OTU29
Phialophora
70%
Fusarium
60%
Biscogniauxia
50%
Sarocladium
40%
Pseudocercosporella
90%
30%
Botryosphaeria
20%
Umbilicaria
10%
Onygenaceae
0%
molecularID
cultureID
Trichosporon
Axis Title
Figure 3.1. Fungal species identified on little brown bats using molecular ID methods
versus culture ID methods. Molecular IDs were generated from five bats from Canoe
Creek State Park and Casselman River Mine; culture IDs were generated from four bats
from Satl Petre Cave and Cudjo’s Cave.
18
Fungi Identified on Indiana Bats
100%
22 genera
6 genera
Zygorhynchus
90%
Mucor
80%
Trichoderma
70%
Sporidiobolus
60%
Penicillium
50%
Golovinomyces
40%
Paecilomyces
30%
Exobasidium
20%
Talaromyces
10%
Aspergillus
0%
molecID
cultreID
Figure 3.2 Fungal species identified on Indiana bats using molecular (molec) ID methods
versus culture ID methods. Molecular IDs were generated from three bats Minor
Rexrode Cave; culture IDs were generated from five bats Salt Petre Cave and Cudjo’s
Cave.
Molecular Analysis of Fungal Populations on Bat Pelage
Fur was collected and DNA was extracted as described. Although the quantities
of DNA isolated from the fur were extremely low (usually below 10 ng/μL) we attempted
PCR using fungal specific primers to amplify the fungal ITS sequence from the fur
clippings. In some cases the initial PCR was successful and the product was used for
cloning and OTU identification; however, in a number of samples, particularly those
collected from Casselman River Mine, this initial PCR was unsuccessful. We
hypothesized that the reaction failed due to the presence of PCR inhibitors, which are
extremely common in environmental samples (Schrader et al. 2012) and present a
problem when attempting to use PCR to amplify small quantities of DNA. We tested this
19
hypothesis by examining the DNA extraction for the presence of PCR inhibitors in a
positive control with bacterial DNA. To do this, sample DNA was mixed with bacterial
DNA known to amplify using fungal specific primers. Figure 2.1 shows the results for a
reaction that contained inhibitors. In the gel, the fungal DNA, which was isolated from
big brown bat 3 from Casselman River Mine (CRM BB3), by itself does not amplify, as
indicated by the lack of banding in lane 1. The mixed environmental DNA alone does
amplify at two different concentrations, as indicated by the presence of bands in lanes 4
and 5. When the environmental and fungal DNA are mixed, however, amplification fails
(no band in lanes 2 or 3); this indicates that there are inhibitors present in the fungal DNA
sample that are inhibiting the amplification of the fungal DNA isolated from a cave
environment. The samples that tested positive for PCR inhibitors were amplified using a
nested PCR approach, which increases the likelihood of successful amplification via the
dilution of inhibitor concentrations. This protocol resulted in the successful amplification
of most samples, barring those collected from Casselman River Mine; these samples were
most likely contaminated by residual heavy metals and humic acids exposed during the
period of operational mining.
20
Figure 3.3. Sample PCR inhibition gel. MWT = Molecular Weight Ladder; lane 1 =
fungal DNA islolated from the fur of big brown bat 3 (CRM BB3); lane 2 = CCSP BB3
fungal DNA + mixed environmental DNA (CP69 at [1 x 10-2]; lane 3 = CCSP BB3
fungal DNA + CP69 at [1 x 10-3]; lane 4 = CP 69 at [1 x 10-2]; lane 5 = CP69 at [1 x 103
]; lane 6 = nuclease free water (negative control).
In addition to dilution and nested PCR, we attempted to remove these inhibitors
from the DNA extraction prior to amplification. We first tried to extract the inhibitors
from the DNA solution via EDTA/EGTA dialysis, during which chelating agents bind to
and sequester metal ions, such as calcium and iron,that prevent amplification. Samples
that had been subjected to the EDTA/EGTA dialysis failed to amplify via PCR in all
cases, most likely because fluid entered the dialysis tube during the extraction and diluted
the already low concentrations of DNA to unamplifiable levels. We next attempted to
remove the PCR inhibitors via ethanol purification and precipitation, but again, PCR
21
amplification of these samples failed in all cases, most likely because samples contained
so little DNA that it was lost completely during the precipitation.
Because the removal of inhibitors had been entirely unsuccessful, we decided to
manipulate the PCR reaction mixture in an attempt to increase specificity and yield. We
began by adding extra MgCl2 to the reaction mixture, which is required as a co-factor and
is important for successful amplification. Some PCR inhibitors, like calcium and iron,
act by competitively binding taq polymerase, preventing magnesium from binding and
thus causing the reaction to fail (Schrader et al. 2012). Increasing the concentration of
magnesium can sometimes displace these inhibitors, allowing taq to perform correctly
and increasing product yield. The addition of MgCl2 alone was insufficient to promote
amplification, so Betaine was also added to the reaction mixture, which seemed to inhibit
the amplification of positive control DNA. We suspected that betaine was binding to and
sequestering a necessary reagent in the 2X Taq MasterMix; however, we have had
success in previous studies using Betain with TaqGold. We therefore tried using a
polymerase with a higher DNA binding affinity.
A high fidelity PCR kit using betaine and TaqGold, an enzyme capable of
amplifying low-level target sequences with high specificity and increasing product yield,
was used in place of Taq 2X master mix (New England Biolabs, Ipswich, MA).
Unfortunately, the TaqGold/Betaine kit failed to amplify any of the remaining samples.
We therefore speculated that betaine, which facilitates strand separation by equalizing the
contribution of GC- and AT-base pairing, could have acted as an inhibitor by preventing
primer binding or strand re-coupling due to an average or low GC content in the sample
DNA.
22
Finally, we attempted PCR using another AccuPrime Taq Polymerase in an
MgSO4/EDTA buffer. The AccuPrime Taq is a recombinant polymerase enzyme that
results in a nine-fold increase in fidelity over Taq alone, and the MgSO4/EDTA buffer
was specifically designed to bind and remove competitive inhibitors. Using this method
we successfully amplified the remaining fungal samples for which we still had DNA.
Table 3.2 summarizes the chemicals and techniques employed to achieve successful
amplification, as well as the results of each technique.
Table 3.2 Techniques and Chemicals used to overcome PCR inhibition. (+) =
successful; (-) = unsuccessful.
Techniques and Chemicals Used to Remove or Overcome Inhibitors
MgCl2 to PCR mix
BSA to PCR mix
Betaine to PCR mix
Betain + TaqGold
Inhibitor extraction via EDTA/EGTA dialysis
1/1- NaOAc/Ethanol Precipitation of DNA
BSA to Exraction mix
+
AccuPrime Taq + MgSO4/EDTA buffer
+
Generation of Fungal Profiles
The species for which we have profiled the fungal communties include Myotis
lucifugus (little brown bat), Myotis sodais (Indiana bat), Myotis leibii (Eastern smallfooted bat), Eptesicus fuscus (big brown bat), and Corynorhinus townsendii virginianus
(Virginia big-eared bat). Table 3.3 summarizes the total number of fur samples
processed via molecular methods by location, bat species, and bat gender. We were
unable to generate fungal profiles for the following bats: five little brown bats (CCSP
LB1, LB2, LB4, LB6, and LB7) from Canoe Creek State Park, two big brown bats (CRM
BB2 and BB3) and one Eastern tri-colored bat (CRM PIP1) from Casselman River Mine,
and two Virginia big-eared bats (COTO4 and COTO5) from Minor Rexrode Creek either
23
because fur clippings were not taken or amplifiable DNA was not obtained (Tables 2.12.4). In total, we identified the fungal communities of 23 bats out of the 31 bats sampled
using molecular techniques. Table A1 in the Appendix lists the fungal OTUs identified
for each bat. For the majority of bats sampled we were able to create a fungal profile
consisting of approximately 94 cleaned ITS sequences. Three big brown bats bats from
Casselman River Mine (CRM BB4, CRM BB5, CRM BB6), have smaller fungal profiles
because a restriction enzyme digest (RFLP) indicated that their commensal populations
possessed lower diversity than necessitated by sending a full 96 well plate for
sequencing; however, the fungal OTUs identified showed a greater diversity than
assumed.
24
Table 3.3 Summary of all samples processed successfully for molecular analysis. CCSP
= Canoe Creek State Park; CRM = Casselman River Mine; MRC = Minor Rexrode
Creek; SHC = Schoolhouse Cave. LB = little brown (bat); BB = big brown (bat); ESF =
Eastern small-footed (bat); MYSO = Indiana bat; COTO = Virginia big-eared bat.
Location
Species
Gender
CCSP
CCSP
CCSP
CCSP
CRM
CRM
CRM
CRM
CRM
CRM
CRM
MRC
MRC
MRC
MRC
MRC
MRC
SHC
SHC
SHC
SHC
SHC
SHC
LB3
LB5
LB8
BB1
BB4
BB5
BB6
BB7
ESF2
LB9
LB10
MYSO1
MYSO2
MYSO3
COTO6
COTO7
COTO8
COTO7
COTO8
COTO9
COTO10
COTO11
COTO12
F
M
M
F
M
F
F
M
M
F
M
M
M
M
M
F
F
M
M
M
M
F
F
Once we identified the fungal community present on each bat, pie charts were
constructed to illustrate the proportion of the commensal community represented by
each OTU. Figure 3.4 displays the composition of the fungal communities of each
bat sampled.
25
26
Figure 3.4. Fungal community of each bat sampled. Figures 3.3a – 3.3d represent the
fungal communities of bats from Canoe Creek State Park: a = little brown bat 3, b = little
brown bat 5, c = little brown bat 8, d = big brown bat 1. Figures 3.3e – 3.3k represent the
communities of bats from Casselman River Mine: e = big brown bat 4, f = big brown bat
5, g = big brown bat 6, h = big brown bat 7, i = Eastern small-footed bat 1, j = little
brown bat 9, k = little brown bat 10. Figures 3.3l – 3.3r represent the profiles of bats from
Minor Rexorode Creek: l = Indiana bat 1, m = Indiana bat 2, n = Indiana bat 3, o =
Virginia big-eared bat 6, p = Virginia big-eared bat 7, q = Virginia big-eared bat 8, r =
Virginia big-eared bat 9. Figures 3.3s – 3.3x represent profiles of bats from Schoolhouse
Cave: s = Virginia big-eared bat 7, t = Virginia big-eared bat 8, u = Virginia big-eared bat
9, v = Virginia big-eared bat 10, w = Virginia big-eared bat 11, x = Virginia big-eared bat
12.
A great deal of variation in the diversity of the fungal communities can be seen
even between bats of the same species sampled from the same location. For example the
highest diversity was seen on a little brown bat from Canoe Creek State Park (CCSP LB
27
8; Figure 3.4c), from which 33 fungal OTUs were identified, while only three OTUs were
identified on a second little brown bat of the same species from the same location (CCSP
LB3; Figure 3.4a). The lowest diversity was observed on an Indiana bat from Minor
Rexrode Cave (MRC COTO9), on which only two OTUs were found; this bat appeared
to be an outlier, as the other Virginia big-eared bats from that location (MRC COTO6,
Figure 3.4o; MRC COTO7, Figure 3.4p; MRC COTO8, Figure 3.4q) possessed an
average of 22 OTUs. Some species of fungi seem to represent a greater proportion of the
commensal populations of these bats than would be expected if they were simply picking
them up from the environment and passively transporting them on their fur. Species
which appear to make up a large portion of the fungal communities of multiple bats
include; Trametes gibbosa, Sporidiobolus salmonicolor, Leucosporidium drummii,
Debaryomces udenii, and members of the genus Pseudogymnoascus. We therefore
decided to combine the fungal profiles of each bat (Appendix Table A1) and analyze
them according to sample site and bat species in order to determine whether the species
of bat or the environment in which it lives is the major contributor to the fungal species
present on the fur.
Figures 3.5 – 3.8 present the fungal OTUs identified on each bat by location.
Because we observed few similarities in the presence or absence of specific fungal
species on bats based on their location, we decided to compare the fungal communities of
bats by species. Figures 3.9 – 3.11 show the fungal communities of each grouped by bat
species. Eastern small-footed bats are not included in these comparisons because only
one bat of that species was sampled, and Indiana bats were not included as all three came
from the same location. We found that certain species of fungi tend to correspond with
28
the species of the bat, rather than their sampling location. The clearest example of this
trend can be seen by comparing the fungal profiles of Virgninia big-eared bats from
Minor Rexrode Cave and Schoolhouse Cave with Indiana bats from Minor Rexrode
Cave; similarities are seen between bats of the same species rather than the same location.
Fungi Found on Bats from
CCSP
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
CCSP LB3
CCSP LB5
CCSP LB8
CCSP BB1
Figure 3.5 A comparison of the fungal OTUs identified on bats from Canoe Creek State
Park (CCSP). LB = little brown bat; BB = big brown bat. The legend to the right of the
figure denotes the species which make up large proportions on individual or shared
fungal community profiles.
29
Fungi Found on Bats from CRM
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
CRM LB9
CRM
LB10
CRM
BB4
CRM
BB5
CRM
BB6
CRM
BB7
CRM
ESF2
Figure 3.6 A comparison of the fungal OTUs identified on bats from Casselman River
Mine (CRM). LB = little brown bat; BB = big brown bat; ESF = Eastern small-footed
bat. The legend to the right of the figure denotes the species which make up large
proportions on individual or shared fungal community profiles.
Fungi Found on Bats from MRC
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
MRC
COTO6
MRC
COTO7
MRC
COTO8
MRC
COTO9
MRC
MYSO1
MRC
MYSO2
MRC
MYSO3
Figure 3.7 A comparison of the fungal OTUs identified on bats from Minor Rexrode
Cave (MRC). COTO = Virginia big-eared bat; MYSO = Indiana bat. The legend to the
right of the figure denotes the species which make up large proportions on individual or
shared fungal community profiles.
30
Fungi Identified on Bats from
Schoolhouse Cave
100%
80%
60%
40%
20%
0%
SHC COTO7
SHC COTO8
SHC COTO9 SHC COTO10 SHC COTO11 SHC COTO12
Figure 3.8 A comparison of the fungal OTUs identified on bats from Schoolhouse Cave
(SHC). COTO = Virginia big-eared bat. The legend to the right of the figure denotes the
species which make up large proportions on individual or shared fungal community
profiles.
Fungi Found on Little Brown
Bats
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
CCSP LB3
CCSP LB5
CCSP LB8
CRM LB9
CRM LB10
Figure 3.9 A comparison of the fungal OTUs identified on all little brown bats (LBs)
sampled. CCSP = Canoe Creek State Park; CRM = Casselman River Mine. The legend
to the right of the figure denotes the species which make up large proportions on
individual or shared fungal community profiles.
31
Fungi Found on Big Brown Bats
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
CCSP BB1
CRM BB4
CRM BB5
CRM BB6
CRM BB7
Figure 3.10 A comparison of the fungal OTUs identified on all big brown bats (BBs)
sampled. CCSP = Canoe Creek State Park; CRM = Casselman River Mine. The legend
to the right of the figure denotes the species which make up large proportions on
individual or shared fungal community profiles.
Fungi Idenified on Virginia BigEared Bats
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
MRC
MRC
MRC
MRC
SHC
SHC
SHC
SHC
SHC
SHC
COTO6 COTO7 COTO8 COTO9 COTO7 COTO8 COTO9 COTO10 COTO11 COTO12
Figure 3.11 A comparison of the fungal OTUs identified on all Virginia big-eared bats
sampled. MRC = Minor Rexrode Cave; SHC = Schoolhouse Cave. The legend to the
right of the figure denotes the species which make up large proportions on individual or
shared fungal community profiles.
32
Because there are fungal OTUs that are common to multiple bat species, we
wanted to determine what types of fungi are most commonly shared. Table 3.5, presents a
summary of each overlapping fungal species by bat species and location. The species in
green, blue, and pink boxes represent plant pathogens, saprophytic filamentous fungi, and
yeast respectively. We found that out of all 123 fungal species identified, only three
species are common to all four locations; these species include Cladosporium
cladosporioides, Stereum complicatum, and Sporidiobolus salmonicolor. When
examined by bat species, we found that of these three species, only S. salmonicolor was
present on at least one individual of every species of bat sampled. Both
Pseudogymnoascus and Pd were found at Casselman River Mine and Minor Rexrode
Creek. We found that members of the genus Pseudogymnoascus were present on three
bat species, including Indiana bats and Virginia big-eared bats, whereas Pd was present
only on Virginia big-eared bats and little brown bats. Finally, this table shows that the
majority of fungi present on multiple species of bats appear to consist of facultative and
full saprotrophs; however, a smaller proportion of the shared population consists of
yeasts, including S. salmonicolor, which is the only species of fungus present on all five
bat species and in all four locations. One of the most prevalent yeasts identified was
Debaryomeces udenii, which made up a total of 54% of all OTUs identified for the
Virginia big-eared bats, and up to 99% of the OTUs for a single bat (SHC COTO7).
33
Table 3.4. Distribution of fungal isolates from five species of bats and four locations. LB
= little brown [bat]; MYSO= Indiana [bat]; BB = big brown [bat]; COTO = Virginia bigeared bat. CCSP = Canoe Creek State Park; CRM = Casselman River Mine; MRC =
Minor Rexrode Creek; SHC = School House Cave. A gray box indicates the presence of
this species in the commensal population of bat or at that location. Green box = plant
pathogen; blue box = saprophytic filamentous; pink = yeast; red = Pseudogymnoascus
and Pd.
Bat Species
Fungal Species
COTO
MYSO
Location
ESF
Cladosporium cladosporioides
Stereum complicatum
Alternaria alternata
Trametes gibbosa
Piptoporus betulinus
Hyphodontia cf sambuci
Lecanicillium fungicola
Stereum sanguinolentum
Penicillium sp.1
Mortierella sp. 2
Cladosporium sphaerospermum
Toxicocladosporium irritans
Aspergillus conicus
Verticillium leptobactrum
Pseudocercosporella fraxini
Mortierella sp. 3
Kernia sp. 2
Cytospora mali
Pseudogymnoascus destructans
Pseudogynmoascus sp.
Debaryomyces hansenii
Trichosporon asahii
Leucosporidium drummii
Debaryomyces fabrii
Malassezia pachydermatis
Debaryomyces maramus
Sporidiobolus salmonicolor
Debaryomyces udenii
34
LB
BB
CCSP
CRM
MRC
SHC
Because yeast are common commensal organisms, these findings lead us to
believe that some members of the fungal communities identified on the bats might
represent true commensals. This hypothesis is strengthened by the finding that bat
species seems to play a larger role in the composition of the fungal community than the
location from which the bat was sampled. In order to conclusively determine if bat
species is truly the more important variable in fungal community composition, we created
a dendrogram based on Bray-Curtis dissimilarities (Figure 3.12). This statistic can
examine the fungal community of of each bat and generate groupings based only upon
the presence or absence of fungal species; if bats are grouped by species, it means that the
presence of certain fungal species is due to the species of that bat rather than its location.
The Bray–Curtis dissimilarity is bound between 0 and 1, where 1 means two
communities share all fungal species, and 0 means that the two sites share no fungi.
The dendogram (Figure 3.12) indicates that certain fungi cluster by bat species,
with the strongest fungal cluster associated with Virginia big-eared bats. In contrast, the
locations of the bats appear not to correlate as stongly with the composition of the fungal
community, as indicated by the large distance between Virginia big-eared bats (MRC
COTO6, COTO7, COTO8) and Indiana bats (MRC MYSO1, MYSO2, MYSO3) from
Minor Rexrode Cave, and the tight clustering of the Virginia big-eared bats from both
locations. Finally, when the species mortality rate is compared to the distances displayed
on the dendogram, it can be seen that bats with lower susceptibilities and mortality rates
are more closely related when grouped by their fungal communities. For example,
Virginia big-eared bats (mortality rate = 0%) are the most distant bat species on the tree,
whereas big brown bats (mortality = 41% ) and Indiana bats (mortality rate = 72%) group
35
more closely with each other than with little brown bats (mortality rate = 91%). When
the fungal OTUs common to the species with lower morality rates are compared, such as
Virginia big-eared bats and big brown bats, w e find that ye ast species make up a large
proportion of their total identified OTUs.
Figure 3.12 A cluster dendrogram based on Bray-Curtis dissimilarities between
individual bat communities based on the presence or absence of different fungal species.
LB = little brown [bat]; MYSO= Indiana [bat]; BB = big brown [bat]; COTO = Virginia
big-eared bat. CCSP = Canoe Creek State Park; CRM = Casselman River Mine; MRC =
Minor Rexrode Creek; SHC = School House Cave. The gender of the bat is indicated by
an m (male) or f (female).
36
CHAPTER V
DISCUSSION
For this study we were interested in the fungal communities present on bat pelage.
Currently, the standard method used for the identification of fungal species found on bats
is a cultivation method: swab samples from bats are used to inoculate various types of
media that are enriched for fungal growth (Sachanowicz et al, 2014; Chaturvedi et al.,
2010; Gargas et al., 2009; Vanderwolf et al., 2013). These plates are then incubated at
low temperatures conducive to the growth of phsychrophilic fungi like Pd. However, this
severely limits the number of identifiable fungal isolates as 99.9% of microorganisms
cannot be grown in culture; the growth conditions selected are designed to facilitate the
growth of psychrophilic fungi, further limiting what species could potentially be
identified. We therefore decided to compare the fungal operational taxonomic units
(OTUs) identifiable from bat fur by molecular methods verus isolates identified by
cultivation to determine which method best represents the fungal communities. The
fungal communities were analyzed for comparison via cultivation and molecular methods
on two bat species, the little brown bat and the Indiana bat.
For both bat species sampled, we found that the fungal communities identified by
molecular methods were far more diverse than those identified via cultivation. On little
brown bats, 51 genera of fungi were identified using molecular methods, while only five
genera were identified via culture (Figure 3.1). Three of the five genera identified by
37
cultivation were also identified using molecular methods alone. Twenty-two genera of
fungi were identified via molecular techniques on Indiana bats, while only 6 were
identified in culture (Figure 3.2). Four of the six cultured isolates were also identified via
molecular methods. All of the fungi identified via cultivation are saprophytes commonly
present in the environment; they are fast growing filamentous fungi which normally
produce large amounts of spores during reproduction. In contrast, many of the genera
identified via molecular methods, such as Leucosporidium and Sporidiobolus, are yeasts;
yeasts tend to grown more slowly in culture and are often outcompeted early on by
rapidly growing fungi such as Mucor and Penicillium. These data indicate that molecular
methods are more sensitive for sampling species which are present in lower quantities on
bats and thus represent low biomass nucleic acid availability. Additionally, molecular
methods are able to detect species which may represent true commensals, such as yeasts,
which are more difficult to culture; cultivation methods most likely identify only fungi
that are easily cultured and grow more rapidly, such as mass spore formers that are
simply being passively transported on bat fur. Finally, when we compared cultivation
versus molecular methods for the identification of Pseudogymnoascus on individual bats
we found the molecular techniques were equally if not more sensitive for the
identification of Pseudogymnoascus and Pd when compared to culture dependent
techniques.
Based on these findings, we used a molecular approach to examine the fungal
populations present on bat fur, and unfortunately found that many fur samples were
contaminated with PCR inhibitors that prevent DNA amplification. PCR inhibitors,
which are extremely common in environmental samples and present a problem when
38
attempting to PCR small quantities of DNA, include a diverse array of molecules which
act in various ways to prevent amplification of nucleic acids. The majority of the
samples containing inhibitors were taken from an inactive section Casselman River Mine,
which was primarily mined for coal from the 1800’s to the early 1900’s, but has sections
which remain active today. Environmental samples such as these may contain inhibitors
such as humic acid, metal ions, and calcium. These inhibitors block PCR by binding to
nucleic acids and enzymes, reduce the specificity of primers, and inhibit DNA
polymerase activity (Schrader et al. 2012).
We used a variety of methods in an attempt to overcome or remove PCR
inhibitors (Table 3.2); however, only the combination of using nested PCR, adding BSA
to the extraction buffer, and using the high fidelity AccuPrime Taq polymerase was
successful at amplifying the most heavily contaminated samples. This combination
allowed us to remove competitive inhibitors at multiple steps: the BSA added to the
Yeastar genomic DNA lysis buffer removed inhibitors during the extraction, while the
Accuprime Taq MgSO4/EDTA buffer chelated them in the PCR reaction mixture and
provided the necessary cofactors for the polymerase. In combination with the AccuPrime
Taq, a recombinant polymerase enzyme capable of a nine-fold increase in fidelity over
regular taq, amplification was finally successful.
Finally, the most crucial action in ensuring successful PCR amplification was
eluting the DNA from the Zymo spin column in a large volume of water rather than a
small one, regardless of the low biomass. Samples eluted in 20-40 μL dH2O that had
amounts of nucleic acids undetectable to a NanoDrop were more likely to be amplified
than samples eluted in 6-10 μL with detectable amounts of DNA. This may indicate that
39
the best time to remove or dilute PCR inhibitors is when the DNA is initially extracted,
and that preventing the early strong binding of certain ions to the DNA can facilitate their
later removal.
Figures 3.5 – 3.8 present the fungal OTUs identified on each bat by location. The
highest diversity is seen at Minor Rexrode Cave, followed by Canoe Creek State Park,
Casselman River Mine, and Schoolhouse cave. We found that in general, the
communities of fungal isolates identified in this study do not correlate strongly with the
location of the sample. Some species of fungi appear to be common in certain locations
despite the species of bat on which they were found, such as the appearance of Trametes
gibbosa on three little brown bats (CCSP LB1, LB5, LB8) and one big brown bat (CCSP
BB1) from Canoe Creek State Park. A small number of fungal OTUs were in fact
present at all four sampling locations; these include Cladosporium cladosporioides,
Stereum complicatum, and Sporidiobolus salmonicolor, which was also the only species
of fungus identified on at least one member of every bat species. C. cladosporioides,
which is present throughout the environment, is one of the most common dermatiaceous
mold contaminants recovered by clinical laboratories (Howard 2012). It has been
isolated from air, soil, foods, building materials, and several other substrates including
both living and decaying plant matter (Bensch et al., 2010). S. complicatum is a
basidiomycete which grows year round on the underside of branches and twigs of trees in
eastern and central North America, where it is known to cause whiterot of hardwoods
(Miller and Miller, 2006). Members of the genus Sporidiobolus have been described as
ubiquitous cosmopolitan yeasts that have been isolated from the stems, logs (Hutchison
and Hiratsuka, 1994), and leaves (Libkind et al. 2005) of trees.
40
The pervasive presence of all three of these fungal species throughout the
environment may suggest that the location of the bat has little influence over the
composition of its fungal community. This hypothesis is strengthened by the fact that all
three species are commonly found growing in wood and plant matter, which is frequently
washed into caves, giving bats ample time for exposure during hibernation. Additionally,
although these fungal species were identified at each location, they generally represent a
small proportion of the overall fungal community. For example, S. complicatum was
found on only one bat from Canoe Creek State Park (CCSP LB8), one bat from
Casselman River Mine (CRM LB9), one bat from Minor Rexrode Cave (MRC COTO9),
and two bats from Schoolhouse Cave (SHC COTO9 and COTO10). C. cladosporioides
was identified on one bat from Canoe Creek State Park (CCSP LB8), two bats from
Casselman River Mine (CRM LB9 and LB10), six out of seven bats from Minor Rexrode
Cave (MRC COTO6, COTO7, COTO8, MYSO1, MYSO2, and MYSO3), and three of
six bats from Schoolhouse Cave (SHC COTO8, COTO11, and COTO12); however, the
genus represented a significant proportion of the fungal communities only on the Indiana
bats from Minor Rexrode Cave (MRC COTO6, COTO7, COTO8), representing an
average of 2.5% for other bats species compared to an average of 55% for the Indiana
bats. These data indicate that while the location of the bat can influence the composition
of their fungal communities, the species of bat might be a better predictor of fungal
community.
While S. salmoicolor was identified at every location and on every species of bat,
some species only had a single individual upon which S. salmoicolor had been identified,
while other species had multiple representative individuals whose communities were
41
made up of a larger proportion of S. salmoicolor OTUs. For example, we found that S.
salmoicolor was present on multiple individuals of two species: little brown bats and
Virginia big-eared bats. Of these two species, only little brown bats (CCSP LB8, CRM
LB9, CRM LB10) possessed a significant number of S. salmoicolor OTUs (29%
compared to 2.9% of fungal OTUs). Finally, the fungal community of the Eastern smallfooted bat from Casselman River Mine was composed largely of S. Salmonicolor (66.7%).
Again, these data seem to indicate that while the location may influence the species of
fungi which are likely to be present, the incidence of that fungi appearing on a specific
bat in significant amounts is more closely linked to the species of the bat.
When we examine the fungal communities of bats by species, this trend becomes
even more evident. Certain fungi which appear prevalent within a particular bat species
also seem to be shared across species. These fungi include Leucosporidium drummi,
which was found on big brown bats, little brown bats, and Indiana bats; Piptoporus
betulinus, found on big brown bats, Eastern small-footed bats, and little brown bats;
Cladosporium cladosporioides, Indiana bats, little brown bats, and Virginia big eared
bats; and Sporidiobolus salmonicolor, found on all bat species. However, some bats
appear to possess fungal species which depend on the species of the bat, rather than the
location. For instance, Mortierella was found to represent a large proportion of the
fungal communities of three out of four Virginia big-eared bats from Minor Rexrode
Creek (MRC COTO6, COTO7, COTO8) but none of the Indiana bats from that location,
while Leucosporidium drummii was found on all of the Indiana bats (MRC MYSO1,
MYSO2, MYSO3) but none of the Virginia big-eared bats.
42
Additionally, one little brown bat from Canoe Creek State Park (CCSP LB8)
shares two fungal OTUs with the other two little brown bats from that location (CCSP
LB3 and LB5), and three different fungal OTUs with the little brown bats from
Casselman River Mine (CRM LB9 and LB10). The big brown bats from the two
locations sampled (CCSP and CRM) share no common fungal species, but when the
fungal communities big brown bats from Casselman River Mine are compared to other
species of bats from the same location (Figure 3.6) it is obvious that there are species
specific differences that make their fungal communities unique to their species. The
same can be said for the comparison of Indiana bats and Virginia big-eared bats from
Minor Rexrode Cave (Figure 3.7). Furthermore, while some species of fungi appear
more frequently on bats of the same species that are also from the same location, we see
that many of these fungi are shared at least to a lesser degree with bats of the same
species from different locations. This is made most evident with the Virginia big-eared
bats (Figure 3.11): while it is obvious that the Virginia big-eared bats from Schoolhouse
Cave have extremely similar fungal communities, it can also be seen that they share
many fungal OTUs with the Virginia big-eared bats from Minor Rexrode Cave, which
posess profiles vastly different from the Indiana bats from Minor Rexrode Cave. Across
these two locations, Virginia big-eared bats share a total of fifteen out of thirty fungal
OTUs common only to Virginia big-eared bats. In contrast, the Indiana bats from Minor
Rexrode Cave share only five species with the Virginia big-eared bats belonging to that
site. These data provide additional support that while location impacts the pervasiveness
of certain fungal species, the species of bat is the most influential factor on whether or
not that fungal OTU will make up a significant proportion of its fungal community.
43
When the fungal communities were used to generate a dendrogram based on
Bray-Curtis dissimilarities (Figure 3.12), the presence or absence of certain fungal OTUs
grouped bats more closely by species than location. Although we do see the Virginia bigeared bats from Schoolhouse Cave all grouping on a single branch, the Virginia big-eared
bats from Minor Rexrode Cave are most closely related to that group, as opposed to the
Indiana bats from the shared location. This indicates that while location can influence
whether certain fungal OTUs will be present on any given bat, bat species is the most
significant factor for fungal community composition. This analysis provides exceedingly
strong support for the hypothesis that bats play host to true commensal populations that
are species specific. Furthermore, the clustering of certain bat species is correlated with
their mortality rates. This may indicate that the species of bats with more defined
commensal populations, particularly the Virginia big-eared bats, have reduced mortality
from WNS, supporting the supposition that commensal populations can confer resistance
to Pd. Additionally, when we examined the species of fungi which are shared by
particular bat species, we find that those bat species that cluster on the dendogram with
low mortality rates are disproportionally colonized by yeasts. Virginia big-eared bats, the
species with the lowest mortality rate, are heavily colonized by one particular species of
yeast, D. udenii, which makes up a total of 55% of all OTUs identified on the Virginia
big-eared bats (Figure 4.1).
44
41%
0%
75%
91%
72%
COTO
ESF
LB
MYSO
100%
90%
80%
70%
60%
50%
40%
30%
20%
10%
0%
BB
Candida psychrophila
Cryptococcus cf cellulolyticus
Leucosporidium drummii
Debaryomyces fabryi
Debaryomyces maramus
Debaryomyces hansenii
Debaryomyces udenii
Mastigobasidium intermedium Sporidiobolus salmonicolor
Figure 4.1 The fungal communities of all bat species, presented with yeast species in
color (filamentous fungi in greyscale) and the mortality rates of each bat species
presented above the bars. BB = big brown bats; COTO = Virginia big-eared bats; ESF =
Eastern small-footed bats; LB = little brown bats; MYSO = Indiana bats.
D. udenii belongs to a genus of psychro- and halo-tolerant yeasts (Breuer and
Harms, 2006) capable of growth between 5oC and 10oC with optimal growth between 2025oC (Skinner et al. 1980), in up to 25% NaCl (Butinar et al., 2005). These findings,
along with the ability of Debaryomyces to grow at a pH range of 3.0 - 10.0 (Breuer and
Harms, 2006), supports the hypothesis that it is a potential bat commensal, as it is
reasonable to assume that the chemistry of bat skin is within these ranges; additionally,
bat skin fluctuates over similar temperature ranges as the bat undergoes cycles of torpor
and arousal over winter hibernation (Reeder et al., 2012). A close relative of D. udenii
(Debaryomyces hansenii) is capable of producing substances such as volatile acids,
alcohols (Arfi et al., 2002), and aldehydes (Dura et al., 2004) that can inhibit the growth
of bacteria, as well as heat stable killer toxins with activities against other yeast species
45
(Gunge et al., 1993) that have increased lethalities at higher salt concentrations (Llorente
et al., 1997; Marquina et al, 2001). This information lends support to the theory that D.
udenii is a commensal that may be acting in a protective role against WNS.
Finally, D. udenii is also categorized as an oleophilic yeast capable of
accumulating more than 20-25% (up to 70%) of its biomass as oil (Breuer and Harms,
2006). In the field, researchers have observed the accumulation of an oily yellow
substance on the fur of Virgnina big-eared bats (Figures 4.2 and 4.3). We hypothesize
that D. udenii is responsible for the production of this oily matter, and that it may be
conferring resistance to WNS. Preliminary competition assays of D. udenii against P.
pannorum indicate that this yeast is capable of slowing or inhibiting fungal growth.
Although additional research is necessary to validate this hypothesis, the amassed data
regarding D. udenii’s physiology and predominate presence on Virgnina big-eared bats, a
species that suffers no mortalities from WNS, indicates that this yeast species is an
important commensal and should be studied further.
F
Figure 4.2 A colony of Virginia big-eared bats in Schoolhouse Cave, West Virginia.
46
Figure 4.3 A sterile cotton swab used to sample the oily, yellow substance on the fur of
the Virginia big-eared bats in Figure 4.2.
47
CHAPTER VI
CONCLUSION
This study has provided insight into presence of commensal fungal species on
bats. This research has shown that bats play host to particular fungal species whose
presence or absence is correlated with the species of bat, and that the presence of specific
commensals may be correlated with the mortality rate of that species. Virginia big-eared
bats, which are heavily colonized by the yeast D. udenii, are currently not susceptible to
WNS. The oleophilic nature of D. udenii may account for this observation although
more research is necessary to validate these claims. By obtaining insight into the
commensal populations of bats with lower mortality rates from WNS, this research will
allow us to predict which bat species or colonies will be most severely affected by
exposure to Pd and allow a targeted approach for future bat conservation efforts. Bats
provide crucial ecosystem services as essential pollinators for economically significant
crops like bananas (Fleming et al., 2009), but the increase in agricultural pests could
negatively impact food production (Boyles et al. 2011). Loss of insectivorous bats could
also lead to a higher prevalence of insect borne diseases. Identifying potential
mechanisms of WNS resistance in bats could be crucial for understanding the future of
the disease within North American bat populations.
48
LITERATURE CITED
Amann, R. I., Ludwig, W., & Schleifer, K. H. (1995). Phylogenetic identification and in
situ detection of individual microbial cells without cultivation.Microbiological
reviews, 59(1), 143-169.
Arfi, K., Spinnler, H., Tache, R., & Bonnarme, P. (2002). Production of volatile
compounds by cheese-ripening yeasts: requirement for a methanethiol donor for
S-methyl thioacetate synthesis by Kluyveromyces lactis. Applied microbiology
and biotechnology, 58(4), 503-510.
Bensch, K., Groenewald, J. Z., Dijksterhuis, J., Starink-Willemse, M., Andersen, B.,
Summerell, B. A., ... & Crous, P. W. (2010). Species and ecological diversity
within the Cladosporium cladosporioides complex (Davidiellaceae,
Capnodiales). Studies in Mycology, 67(1), 1-94.
Blaser, M. J. (1997). Ecology of Helicobacter pylori in the human stomach.Journal of
Clinical Investigation, 100(4), 759.
Blehert, D. S., Hicks, A. C., Behr, M., Meteyer, C. U., Berlowski-Zier, B. M., Buckles, E.
L., ... & Stone, W. B. (2009). Bat white-nose syndrome: an emerging fungal
pathogen?. Science, 323(5911), 227-227.
Bouma, H. R., Carey, H. V., & Kroese, F. G. (2010). Hibernation: the immune system at
rest?. Journal of Leukocyte Biology, 88(4), 619-624.
Boyles, J. G., & Willis, C. K. (2009). Could localized warm areas inside cold caves
reduce mortality of hibernating bats affected by white-nose syndrome?.Frontiers
in Ecology and the Environment, 8(2), 92-98.
Boyles, J. G., Cryan, P. M., McCracken, G. F., & Kunz, T. H. (2011). Economic
importance of bats in agriculture. Science, 332(6025), 41-42.
Breuer, U., & Harms, H. (2006). Debaryomyces hansenii—an extremophilic yeast with
biotechnological potential. Yeast, 23(6), 415-437.
Butinar, L., Santos, S., Spencer‐Martins, I., Oren, A., & Gunde‐Cimerman, N. (2005).
Yeast diversity in hypersaline habitats. FEMS microbiology letters, 244(2), 229234.
49
Casadevall, A., & Pirofski, L. A. (2000). Host-pathogen interactions: basic concepts of
microbial commensalism, colonization, infection, and disease.Infection and
Immunity, 68(12), 6511-6518.
Chaturvedi, V., Springer, D. J., Behr, M. J., Ramani, R., Li, X., Peck, M. K., ... &
Chaturvedi, S. (2010). Morphological and molecular characterizations of
psychrophilic fungus Geomyces destructans from New York bats with white nose
syndrome (WNS). PLoS One, 5(5), e10783.
Cryan, P. M., Meteyer, C. M., Boyles, J. C., & Blehert, D. S. (2010). Wing pathology of
white-nose syndrome in bats suggests life-threatening disruption of physiology.
BMC Biology, 8(135).
Dura, M., Flores, M., & Toldrá, F. (2004). Effect of growth phase and dry-cured sausage
processing conditions on Debaryomyces spp. generation of volatile compounds
from branched-chain amino acids. Food chemistry, 86(3), 391-399.
Fleming, T. H., Geiselman, C., & Kress, W. J. (2009). The evolution of bat pollination: a
phylogenetic perspective. Annals of Botany, 104(6), 1017-1043.
Gargas, A., Trest, M. T., Christensen, M., Volk, T. J., & Blehert, D. S. (2009). Geomyces
destructans sp. nov. associated with bat white-nose syndrome.Mycotaxon, 108(1),
147-154.
Geiser, F., & Ruf, T. (1995). Hibernation versus daily torpor in mammals and birds:
physiological variables and classification of torpor patterns. Physiological
Zoology, 935-966.
Grice, E. A., & Segre, J. A. (2011). The skin microbiome. Nature Reviews
Microbiology, 9(4), 244-253.
Grieneisen, L. (2011). Hibernacula Microclimate and White-nose Syndrome
Susceptibility in the Little Brown Myotis (Myotis lucifugus).
Gunge, N., Fukuda, K., Morikawa, S., Murakami, K., Takeda, M., & Miwa, A. (1993).
Osmophilic linear plasmids from the salt-tolerant yeast Debaryomyces
hansenii. Current genetics, 23(5-6), 443-449.
Howard, D. H. (Ed.). (2012). Pathogenic fungi in humans and animals. CRC Press.
Hutchison, L. J., & Hiratsuka, Y. (1994). Some wood-inhabiting yeasts of trembling
aspen Columbia. Mycologia, 86(3), 386-391.
Johnson, J. B., Edwards, J. W., & Wood, P. B. (2005). Virginia big-eared bats
(Corynorhinus townsendii virginianus) roosting in abandoned coal mines in West
Virginia. Northeastern Naturalist, 12(2), 233-240.
50
Jonasson, K. A., & Willis, C. K. (2011). Changes in body condition of hibernating bats
support the thrifty female hypothesis and predict consequences for populations
with white-nose syndrome. PLoS One, 6(6), e21061.
Kurta, A., & Whitaker Jr, J. O. (1998). Diet of the endangered Indiana bat (Myotis
sodalis) on the northern edge of its range. The American midland naturalist,
140(2), 280-286.
Larcher, G., Bouchara, J. P., Pailley, P., Montfort, D., Beguin, H., De Bievre, C., &
Chabasse, D. (2003). Fungal biota associated with bats in Western France.
Journal de mycologie médicale, 13(1), 29-34.
Libkind, D., Gadanho, M., van Broock, M., & Sampaio, J. P. (2005). Sporidiobolus
longiusculus sp. nov. and Sporobolomyces patagonicus sp. nov., novel yeasts of
the Sporidiobolales isolated from aquatic environments in Patagonia,
Argentina. International journal of systematic and evolutionary
microbiology, 55(1), 503-509.
Llorente, P., Marquina, D., Santos, A., Peinado, J. M., & Spencer-Martins, I. (1997).
Effect of salt on the killer phenotype of yeasts from olive brines. Applied and
environmental microbiology, 63(3), 1165-1167.
Lorch, J. M., Meteyer, C. U., Behr, M. J., Boyles, J. G., Cryan, P. M., Hicks, A. C., ... &
Blehert, D. S. (2011). Experimental infection of bats with Geomyces destructans
causes white-nose syndrome. Nature, 480(7377), 376-378.
Marquina, D., Barroso, J., Santos, A., & Peinado, J. M. (2001). Production and
characteristics of Debaryomyces hansenii killer toxin. Microbiological
research,156(4), 387-391.
Meteyer, C. U., Barber, D., & Mandl, J. N. (2012). Pathology in euthermic bats with
white-nose syndrome suggests a natural manifestation of immune reconstitution
inflammatory syndrome. Virulence, 3(7), 0-1.4.
Miller, Orson K., and Hope Miller. North American mushrooms: a field guide to edible
and inedible fungi. Globe Pequot, 2006.
Minnis, A. M., & Lindner, D. L. (2013) Phylogenetic evaluation of Geomyces and allies
reveals no close relatives of Pseudogymnoascus destructans, comb. nov., in bat
hibernacula of eastern North America.Fungal biology, 117(9), 638-649.
Reeder, D. M., Frank, C. L., Turner, G. G., Meteyer, C. U., Kurta, A., Britzke, E. R., ... &
Blehert, D. S. (2012). Frequent arousal from hibernation linked to severity of
infection and mortality in bats with white-nose syndrome. PloS one, 7(6), e38920.
51
Reichard, J. D., & Kunz, T. H. (2009). White-nose syndrome inflicts lasting injuries to
the wings of little brown myotis (Myotis lucifugus). Acta Chiropterologica, 11(2),
457-464.
Sachanowicz, K., Stępień, A., & Ciechanowski, M. (2014). Prevalence and phenology of
white-nose syndrome fungus Pseudogymnoascus destructans in bats from
Poland. Central European Journal of Biology, 9(4), 437-443.
Schrader, C., Schielke, A., Ellerbroek, L., & Johne, R. (2012). PCR inhibitors–
occurrence, properties and removal. Journal of applied microbiology, 113(5),
1014-1026.
Skinner, F. A., Passmore, S. M., & Davenport, R. R. (1980). Biology and activities of
yeasts. Academic Press.
Turner, G. G., Reeder, D., & Coleman, J. T. (2011). A Five-year Assessment of Mortality
and Geographic Spread of White-Nose Syndrome in North American Bats, with a
Look at the Future. Update of White-Nose Syndrome in Bats. Bat Research News,
13.
Vanderwolf, K. J., McAlpine, D. F., Malloch, D., & Forbes, G. J. (2013). Ectomycota
associated with hibernating bats in Eastern Canadian caves prior to the emergence
of white-nose syndrome. Northeastern Naturalist, 20(1), 115-130.
Wibbelt, G., Kurth, A., Hellmann, D., Weishaar, M., Barlow, A., Veith, M., ... & Blehert,
D. S. (2010). White-nose syndrome fungus (Geomyces destructans) in bats,
Europe. Emerging Infectious Diseases, 16(8), 1237.
Willis, C. K., Menzies, A. K., Boyles, J. G., & Wojciechowski, M. S. (2011). Evaporative
water loss is a plausible explanation for mortality of bats from white-nose
syndrome. Integrative and Comparative Biology, 51(3), 364-373.
52
APPENDIX
TABLES
Table A1. All fungal OTUs identified for each bat. “Well” refers to the location of a
clonal sample containing identical ITS fragments in a 96 well plate. “Query Cover”
refers to the percentage of the sample that overlaps the reference sequence. “%
Identity” refers to the percentage of identical bases shared by the reference
sequence and the query sequence. “Accession #” refers to the NCBI reference
number of the subject sequence. “BLAST Match” indicates the identity of the
reference sequence; the phylum and family of the reference sequence are also listed.
Fungal Profile for CCSP LB3
Well
Query
Cover
%
Identity
A03
90%
99%
A04
98%
99%
A05
96%
99%
A06
96%
99%
A07
96%
99%
A08
89%
99%
A09
100%
99%
A10
78%
98%
A11
100%
99%
A12
96%
99%
B02
96%
99%
B03
99%
99%
B04
96%
99%
B05
100%
B06
x
B07
100%
B08
x
B09
B10
Accesion #
BLAST Match
Phylum
Family
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
99%
JQ700297.1
KC176329.1
KJ140574.1
KJ140574.1
KJ140574.2
KJ140574.3
FJ481048.1
KJ140574.2
FJ481048.1
KJ140574.2
KJ140574.2
JQ700297.1
KJ140574.2
FJ481048.1
99%
99%
99%
99%
99%
B11
99%
98%
B12
99%
99%
C01
100%
99%
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
FJ481048.1
Trametes gibbosa
Basidiomycota
Polyporaceae
FJ481048.1
FJ481048.1
FJ481048.1
KC176329.1
KC176329.1
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
53
C02
99%
99%
C03
100%
99%
C04
100%
99%
C05
100%
99%
C06
100%
99%
C07
96%
99%
C08
100%
99%
C09
100%
99%
C10
100%
99%
C11
98%
97%
C12
99%
99%
D01
100%
99%
D02
100%
99%
D03
100%
99%
D04
96%
99%
D05
100%
99%
D06
100%
99%
D07
96%
99%
D08
96%
99%
D09
96%
99%
D10
99%
100%
D11
100%
99%
D12
100%
99%
E01
100%
99%
E02
99%
99%
E03
100%
99%
E04
100%
99%
E05
99%
99%
E06
100%
99%
E07
100%
99%
E08
100%
99%
E09
100%
99%
E10
100%
99%
E11
96%
99%
E12
100%
99%
F01
100%
99%
F02
100%
99%
F03
99%
99%
F04
100%
99%
F05
100%
99%
F06
100%
90%
F07
100%
99%
F08
99%
99%
JQ700297.1
FJ481048.1
JQ700297.1
JQ700297.2
FJ481048.1
KJ140574.2
JQ700297.2
FJ481048.1
FJ481048.1
JQ700297.1
FJ481048.1
FJ481048.1
JQ700297.2
KC176329.1
KJ140574.2
JQ700297.2
FJ481048.1
KJ140574.2
KJ140574.2
KJ140574.2
FJ481048.1
KC176329.1
JQ700297.1
JQ700297.2
FJ481048.1
FJ481048.1
JQ700297.2
FJ481048.1
JQ700297.2
FJ481048.1
JQ700297.2
FJ481048.1
FJ481048.1
KJ140574.2
JQ700297.2
JQ700297.2
JQ700297.2
FJ481048.1
JQ700297.2
JQ700297.2
KC461307.1
KC176329.1
FJ481048.1
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
54
99%
JQ700297.2
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
99%
JQ700297.2
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
72%
99%
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
G02
100%
99%
Trametes gibbosa
Basidiomycota
Polyporaceae
G03
100%
99%
G04
100%
99%
G05
100%
99%
G06
99%
99%
G07
100%
99%
G08
100%
99%
G09
100%
99%
G10
96%
99%
G11
100%
99%
G12
100%
96%
H01
96%
99%
H02
100%
99%
H03
94%
99%
H04
100%
99%
H05
100%
99%
H06
100%
99%
H07
100%
99%
H08
100%
99%
H09
100%
99%
H10
100%
99%
H11
100%
99%
H12
68%
99%
JQ700297.2
FJ481048.1
FJ481048.1
JQ700297.2
FJ481048.1
KC176329.1
JQ700297.2
JQ700297.2
KC176329.1
KJ140574.2
KC176329.1
JQ700297.1
KJ140574.2
FJ481048.1
FJ481048.1
FJ481048.1
JQ700297.2
JQ700297.2
FJ481048.1
FJ481048.1
FJ481048.1
FJ481048.1
FJ481048.1
KJ140574.2
Well
Query
Cover
%
Identity
A02
88%
92%
F09
100%
F10
x
F11
100%
F12
x
G01
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Corticiaceae
Fungal Profile of CCSP LB8
Accesion #
BLAST Match
Phylum
Family
Devriesia lagerstroemiae
Ascomycota
Teratosphaeriaceae
Devriesia lagerstroemiae
Ascomycota
Teratosphaeriaceae
96%
GU214634.1
GU214634.1
JQ281483.1
A05
99%
JQ768326.1
Ascomycota
Davidiellaceae
A06
99%
JQ768326.1
Cladosporium
sphaerospermum
Cladosporium
sphaerospermum
Ascomycota
Davidiellaceae
97%
GU214634.1
FJ183441.1
Devriesia lagerstroemiae
Ascomycota
Teratosphaeriaceae
89%
Sporidiobolus salmonicolor
Basidiomycota
Meruliaceae
80%
AM901896.1
Uncultured ascomycete
Ascomycota
Ascomycete
99%
DQ267124.1
Botryobasidium botryosum
Basidiomycota
Botryobasidiaceae
A03
A04
96%
61%
A07
A08
45%
A09
x
A10
A11
A12
Entoloma abortivum
x
55
B02
x
B03
90%
97%
B04
99%
B05
99%
B06
99%
B07
99%
B08
89
92%
B09
99%
B10
99%
B11
79%
B12
C01
99%
89%
99%
C02
99%
C03
97%
C04
58%
99%
C05
99%
C06
99%
C07
66%
99%
C08
85%
99%
C09
90%
88%
C10
88%
99%
C11
91%
99%
C12
91%
100%
D01
88%
99%
D02
83%
GU797138.1
EU040243.2
FJ810170.1
FJ914884.1
EU040243.2
AY952467.1
JF706331.1
FJ481048.1
AY265336.1
FJ914884.1
EU486454.1
EU040243.2
GU214634.1
EU040243.2
AY219361.1
FJ914884.1
GU327541.1
EU040243.2
JQ673122.1
FJ914884.1
KJ140584.1
GQ121322.2
AY952467.1
Alternaria alternata
Ascomycota
Pleosporaceae
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
Pleurotus floridanus
Basidiomycota
Pleurotaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
Stilbella fimetaria
Ascomycota
Perenniporia narymica
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Dactylaria ampulliformis
Ascomycota
Orbiliaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Rhodocollybia butyracea
Basidiomycota
Tricholomataceae
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
Devriesia lagerstroemiae
Ascomycota
Teratosphaeriaceae
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
Ceriporiopsis pannocincta
Basidiomycota
Meruliaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Bulleribasidium oberjochense
Basidiomycota
Tremellaceae
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
Skeletocutis nivea
Basidiomycota
Polyporaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Stereum complicatum
Ascomycota
Ascomycete
Alternaria alternata
Ascomycota
Pleosporaceae
Stilbella fimetaria
Ascomycota
Ascomycete
Ascomycota
Davidiellaceae
89%
AJ300335.1
Cladosporium
cladosporioides
D03
x
D04
88%
99%
JN164983.1
Trametes betulina
Basidiomycota
Coriolaceae
D05
91%
99%
FJ481048.1
Trametes gibbosa
Basidiomycota
Polyporaceae
D06
83%
100%
Eutypella scoparia
Ascomycota
Diatrypaceae
D07
86%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
D08
88%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
D09
87%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
D10
91%
99%
Trametes gibbosa
Basidiomycota
Polyporaceae
D11
91%
99%
D12
90%
99%
JN601153.1
FJ914884.1
FJ914884.1
FJ914884.1
KC176329.1
FJ481048.1
JQ673109.1
Trametes gibbosa
Basidiomycota
Polyporaceae
Gloeoporus dichrous
Basidiomycota
Meruliaceae
Ascomycota
Davidiellaceae
E01
94%
99%
AY251074.2
Cladosporium
cladosporioides
E02
90%
99%
Trametes gibbosa
Basidiomycota
Polyporaceae
E03
90%
99%
Skeletocutis nivea
Basidiomycota
Polyporaceae
E04
88%
98%
E05
87%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
E06
89%
99%
Stilbella fimetaria
Ascomycota
Ascomycete
E07
87%
99%
FJ481048.1
JQ673123.1
GU941394.1
FJ914884.1
AY952467.1
FJ914884.1
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Uncultured eukaryote clone
56
E08
91%
99%
E09
87%
99%
E10
87%
99%
E11
x
E12
88%
99%
F01
86%
99%
F02
91%
99%
F03
x
F04
92%
100%
F05
91%
100%
F06
87%
98%
F07
86%
93%
F08
91%
96%
F09
85%
99%
F10
x
F11
80%
95%
F12
88%
99%
FJ810142.1
FJ914884.1
FJ914884.1
EU019284.2
FJ914884.1
FJ810142.1
JN164943.1
GU073119.1
AY740071.1
DQ019350.1
HE649950.1
KJ140584.1
Lepista irina
Basidiomycota
Tricholomataceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Ramularia pratensis
Ascomycota
Mycosphaerellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Lepista irina
Basidiomycota
Tricholomataceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Leptosphaerulina chartarum
Ascomycota
Didymellaceae
Ustilago syntherismae
Basidiomycota
Ustilaginaceae
Mycosphaerella latebrosa
Ascomycota
Mycosphaerellaceae
Ossicaulis lignatilis
Basidiomycota
Lyophyllaceae
Stereum complicatum
Ascomycota
Ascomycete
AB006433.1
AJ969618.1
Aspergillus penicillioides
Ascomycota
Trichocomaceae
Pezizales sp.
Ascomycota
Ascomycete
Cladosporium
cladosporioides
Schizopora cf. radula
Ascomycota
Davidiellaceae
Basidiomycota
Corticiaceae
Sporidiobolus salmonicolor
Basidiomycota
Basidiomycete
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
G01
88%
99%
AY251074.2
G02
87%
99%
G03
93%
100%
G04
84%
99%
G05
88%
77%
G06
83%
99%
G07
87%
99%
G08
89%
99%
JQ673187.1
AF444611.1
FJ914884.1
AF310980.1
FJ914884.1
FJ914884.1
JN164983.1
Fusarium lateritium
Ascomycota
Hypocreales
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Trametes betulina
Basidiomycota
Coriolaceae
Ascomycota
Davidiellaceae
G09
93%
100%
JX139700.1
Cladosporium
cladosporioides
G10
87%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
G11
84%
99%
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
G12
85%
99%
H01
90%
99%
H02
73%
99%
FJ914884.1
EU040243.2
AY558597.1
HQ607815.1
FJ914884.1
Hymenochaete spreta
Basidiomycota
Hymenochaetaceae
Leptosphaerulina chartarum
Ascomycota
Didymellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H03
85%
99%
JX069874.1
Toxicocladosporium
strelitziae
H04
85%
99%
Basidiomycota
Sporidiobolaceae
89%
99%
FJ914884.1
FJ183441.1
Sporidiobolus salmonicolor
H05
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H06
x
H07
87%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H08
86%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H09
87%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H10
88%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H11
90%
99%
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
DQ384584.1
Phlebia tremellosa
Basidiomycota
Corticiaceae
H12
x
Fungal Profile for CCSP BB1
57
Well
Query
Cover
%
Identity
A02
100%
99%
A03
90%
99%
A04
100%
99%
A05
96%
99%
A06
96%
99%
A07
99%
99%
A08
86%
92%
A09
100%
99%
A10
79%
98%
A11
100%
99%
A12
x
B01
x
B02
96%
99%
B03
81%
99%
B04
96%
99%
B05
100%
99%
B06
100%
99%
B07
100%
99%
B08
100%
97%
B09
99%
99%
B10
100%
90%
B11
100%
99%
B12
100%
99%
C01
100%
99%
C02
100%
99%
C03
100%
99%
C04
100%
99%
C05
100%
99%
C06
100%
99%
C07
96%
99%
C08
100%
99%
C09
100%
99%
C10
100%
99%
C11
100%
99%
C12
100%
98%
D01
100%
89%
D02
99%
99%
D03
100%
99%
D04
96%
99%
D05
100%
99%
Accesion #
BLAST Match
Phylum
Family
JQ700297.1
JQ700297.1
FJ481048.1
KJ140574.1
KJ140574.1
FJ481048.1
JN129976.1
FJ481048.1
FJ820503.1
FJ481048.1
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia sp.
Basidiomycota
Hymenochaetaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
KJ140574.1
JQ700297.1
KJ140574.1
FJ481048.1
JQ700297.1
FJ481048.1
FJ481048.1
FJ481048.1
JQ700297.1
FJ481048.1
FJ481048.1
FJ481048.1
JQ700297.1
FJ481048.1
JQ700297.1
JQ700297.1
FJ481048.1
KJ140574.1
JQ700297.1
FJ481048.1
FJ481048.1
JQ700297.1
KC525203.1
FJ481048.1
JQ700297.1
KC176329.1
KJ140574.1
JQ700297.1
Uncultured fungus clone
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
58
D06
100%
99%
D07
96%
99%
D08
96%
99%
D09
96%
99%
D10
100%
99%
D11
100%
99%
D12
87%
90%
E01
99%
99%
E02
99%
99%
E03
100%
99%
E04
91%
98%
E05
100%
99%
E06
100%
99%
E07
100%
99%
E08
100%
99%
E09
100%
99%
E10
100%
99%
E11
96%
99%
E12
100%
99%
F01
100%
99%
F02
100%
99%
F03
100%
99%
F04
100%
99%
F05
100%
99%
F06
100%
90%
F07
100%
99%
F08
100%
99%
F09
100%
99%
FJ481048.1
KJ140574.1
KJ140574.1
KJ140574.1
FJ481048.1
FJ481048.1
JQ700297.1
JQ700297.1
FJ481048.1
FJ481048.1
JQ700297.1
FJ481048.1
JQ700297.1
FJ481048.1
JQ700297.1
FJ481048.1
FJ481048.1
KJ140574.1
JQ700297.1
JQ700297.1
JQ700297.1
FJ481048.1
JQ700297.1
JQ700297.1
KC176329.1
KC176329.1
FJ481048.1
JQ700297.1
F10
X
F11
99%
99%
F12
X
G01
72%
99%
G02
100%
92%
G03
99%
99%
G04
100%
99%
G05
100%
99%
G06
90%
98%
G07
100%
99%
G08
100%
99%
G09
100%
99%
G10
96%
99%
G11
87%
99%
G12
100%
99%
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
JQ700297.1
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
JQ700297.1
FJ481048.1
FJ481048.1
JQ700297.1
FJ481048.1
KC176329.1
JQ700297.1
JQ700297.1
FJ481048.1
KJ140574.1
KC176329.1
JQ700297.1
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
59
H01
96%
99%
H02
100%
99%
KJ140574.1
FJ481048.1
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
FJ481048.1
JQ700297.1
JQ700297.1
FJ481048.1
FJ481048.1
FJ481048.1
FJ481048.1
FJ481048.1
KJ140574.1
Trametes gibbosa
Basidiomycota
Polyporaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Trametes gibbosa
Basidiomycota
Polyporaceae
Hyphodontia cf. sambuci
Basidiomycota
Hymenochaetaceae
H03
x
H04
100%
99%
H05
100%
98%
H06
100%
99%
H07
100%
99%
H08
99%
99%
H09
100%
99%
H10
100%
99%
H11
100%
99%
H12
68%
99%
Well
Query
Cover
%
Identity
Accesion #
BLAST Match
Phylum
Family
A02
96%
99%
FJ914884.1
Sporidiobolus
salmonicolor
Basidiomycota
Sporidiobolaceae
A03
99%
98%
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
A04
97%
99%
A05
100%
99%
A06
100%
99%
A07
96%
91%
A08
60%
99%
A09
96%
91%
A10
100%
99%
A11
96%
91%
A12
98%
100%
NR_077068.1
JX270484.1
KC176306.1
KC176306.1
FN908919.1
KC176306.1
FN908919.1
KC176306.1
FN908919.1
JX270401.1
B01
x
B02
100%
99%
B03
100%
100%
Fungal Profile of CRM BB4
Kernia sp.
Ascomycota
Microascaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Mortierella sp.
Zygomycota
Mortierellaceae
JN689342.1
KC176306.1
Epicoccum nigrum
Ascomycota
Incertae sedis
Trametes versicolor
Basidiomycota
Polyporaceae
plant
Fabaceae
B04
98%
99%
EF432796.1
Pueraria montana var.
lobata
B05
98%
100%
Mortierella sp.
Zygomycota
Mortierellaceae
B06
99%
99%
Trametes versicolor
Basidiomycota
Polyporaceae
B07
99%
98%
Trichosporon loubieri
Basidiomycota
Trichosporonaceae
B08
99%
100%
Trametes versicolor
Basidiomycota
Polyporaceae
B09
57%
100%
JX270401.1
KC176306.1
KC254110.1
KC176306.1
KC176306.1
Trametes versicolor
Basidiomycota
Polyporaceae
Basidiomycota
Tremellaceae
Trametes versicolor
Basidiomycota
Polyporaceae
B10
x
Cryptococcus cf.
cellulolyticus
B11
95%
99%
HQ670761.2
B12
99%
99%
C01
98%
100%
Mortierella sp.
Zygomycota
Mortierellaceae
C02
67%
91%
KC176306.1
JX270401.1
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
HQ670761.2
Cryptococcus cf.
cellulolyticus
Basidiomycota
Tremellaceae
C03
95%
99%
60
C04
96%
91%
C05
100%
98%
C06
99%
97%
C07
99%
99%
C08
100%
94%
C09
99%
100%
FN908919.1
NR_077068.1
EF196809.1
KC176306.1
AB540569.1
KC176306.1
99%
100%
KC176306.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces hansenii
Ascomycota
Debaryomycetaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Acremonium atrogriseum
Ascomycota
Hypocreaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C10
C11
x
Trametes versicolor
C12
x
D01
96%
91%
D02
97%
100%
D03
39%
99%
D04
99%
99%
FN908919.1
JX270501.1
KC311470.1
KC176306.1
Geomyces sp.
Ascomycota
Myxotrichaceae
Epicoccum nigrum
Ascomycota
Incertae sedis
Trametes versicolor
Basidiomycota
Polyporaceae
Fungal Profile of CRM BB5
Well
Query
Cover
%
Identity
D05
90%
91%
D06
94%
91%
D07
99%
99%
D08
66%
98%
D09
90%
99%
D10
90%
99%
D11
96%
91%
D12
96%
91%
E01
69%
90%
E02
69%
90%
E03
96%
91%
E04
96%
91%
E05
96%
91%
E06
90%
99%
E07
96%
91%
E08
96%
91%
E09
96%
91%
E10
96%
91%
E11
96%
91%
E12
99%
99%
Well
Query
Cover
%
Identity
F01
96%
100%
F02
98%
100%
F03
x
Accesion #
BLAST Match
Phylum
Family
FN908919.1
FN908919.1
KC176306.1
KC176306.1
JQ409465.1
JQ409464.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
JQ409464.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
KC176306.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Rigidoporus sp.
Basidiomycota
Meripilaceae
Rigidoporus sp.
Basidiomycota
Meripilaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Rigidoporus sp.
Basidiomycota
Meripilaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Fungal Profile of CRM BB6
Accesion #
BLAST Match
Phylum
Family
KC176306.1
KC176306.1
FN908919.1
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
61
F04
x
F05
70%
100%
F06
x
F07
99%
98%
F08
70%
100%
F09
100%
99%
F10
100%
99%
F11
99%
99%
F12
100%
100%
G01
x
G02
x
G03
97%
100%
G04
97%
100%
G05
100%
99%
G06
100%
99%
G07
x
G08
x
G09
66%
98%
G10
66%
98%
G11
x
G12
x
H01
x
H02
66%
98%
H03
90%
99%
H04
90%
99%
H05
x
H06
x
H07
x
H08
x
FN908919.1
KC176325.1
FN908919.1
NR_077068.1
KC176325.1
KC176306.1
KC176306.1
KC176306.1
KC176306.1
FN908919.1
FN908919.1
JX270501.1
JX270501.1
KC176306.1
KC176306.1
FN908919.1
FN908919.1
KC176344.1
KC176344.1
FN908919.1
FN908919.1
FN908919.1
KC176344.1
JQ409464.1
JQ409464.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces sp.
Ascomycota
Myxotrichaceae
Geomyces sp.
Ascomycota
Myxotrichaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Trametes versicolor
Basidiomycota
Polyporaceae
Rigidoporus sp.
Basidiomycota
Meripilaceae
Rigidoporus sp.
Basidiomycota
Meripilaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Fungal Profile of CRM BB7
Well
Query
Cover
%
Identity
A02
96%
91%
A03
96%
91%
A04
96%
91%
A05
96%
91%
A06
x
A07
96%
91%
A08
96%
91%
A09
96%
91%
A10
96%
91%
A11
96%
91%
Accesion #
BLAST Match
Phylum
Family
FN908919.1
FN908919.1
FN908919.1
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
62
91%
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
96%
91%
Basidiomycota
Leucosporidiaceae
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B04
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B05
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B06
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B07
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B08
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B09
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B10
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B11
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
B12
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C01
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C02
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C03
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C04
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C05
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C06
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C07
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C08
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C09
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C10
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C11
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C12
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
D01
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
D02
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
D03
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
D04
96%
91%
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
D05
100%
98%
Acremonium atrogriseum
Ascomycota
Cephalothecaceae
D06
99%
98%
D07
96%
91%
D08
96%
91%
D09
96%
91%
D10
96%
91%
D11
96%
91%
D12
96%
91%
E01
99%
100%
E02
96%
91%
E03
96%
91%
E04
96%
91%
E05
96%
91%
E06
96%
91%
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
AB540569.1
AB540569.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
JN942672.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
Leucosporidium drummii
96%
A12
96%
B01
x
B02
B03
Acremonium atrogriseum
Ascomycota
Cephalothecaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
63
E07
96%
91%
E08
96%
91%
E09
100%
98%
E10
96%
91%
E11
96%
91%
E12
96%
91%
F01
96%
91%
F02
96%
91%
F03
96%
91%
F04
96%
91%
F05
96%
91%
F06
100%
98%
F07
96%
91%
F08
96%
91%
F09
96%
91%
F10
96%
91%
F11
96%
91%
F12
94%
99%
G01
80%
91%
G02
81%
91%
G03
87%
91%
G04
87%
91%
G05
84%
91%
G06
83%
91%
G07
99%
89%
G08
87%
91%
G09
94%
91%
G10
83%
91%
G11
90%
90%
G12
85%
91%
H01
96%
91%
H02
95%
91%
H03
95%
91%
H04
96%
91%
H05
96%
91%
H06
96%
91%
H07
96%
91%
H08
96%
91%
H09
96%
91%
H10
96%
91%
H11
96%
91%
H12
96%
91%
FN908919.1
FN908919.1
AB540569.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
AB214657.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
EU040243.2
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Acremonium atrogriseum
Ascomycota
Cephalothecaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Verticillium leptobactrum
Ascomycota
Clavicipitaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Toxicocladosporium irritans
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
64
Fungal Profile of CRM LB9
Well
Query
Cover
%
Identity
A02
97%
99%
A03
96%
99%
A04
96%
99%
A05
96%
99%
A06
98%
99%
A07
96%
99%
A08
100%
100%
A09
100%
99%
A10
65%
99%
A11
99%
99%
A12
98%
99%
B02
96%
99%
B03
96%
99%
B04
98%
99%
B05
98%
99%
B06
96%
99%
B07
98%
99%
B08
98%
99%
B09
98%
99%
B10
96%
99%
B11
95%
99%
B12
98%
99%
C01
96%
91%
C02
71%
93%
C03
98%
99%
C04
98%
99%
C05
100%
99%
C06
95%
99%
C07
96%
99%
C08
96%
98%
C09
98%
99%
C10
98%
99%
C11
98%
99%
C12
96%
99%
D01
96%
99%
D02
75%
95%
D03
96%
99%
D04
98%
99%
Accession #
BLAST Match
Phylum
Family
HE588133.1
FJ914884.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
JF439487.1
KF367497.1
FJ914884.1
HE588133.1
HE588133.1
FJ914884.1
FJ914884.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
FJ914884.1
HE588133.1
HE588133.1
FN908919.1
FN908919.1
HE588133.1
HE588133.1
AB369919.1
HE588133.1
FJ914884.1
HE588133.1
HE588133.1
HE588133.1
HF911371.1
FJ914884.1
FJ914884.1
KF477240.1
FJ914884.1
HE588133.1
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Trichosporon asahii
Basidiomycota
Trichosporonaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Onygenaceae sp.
Ascomycota
Onygenaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
65
D05
98%
99%
D06
96%
99%
D07
93%
99%
D08
98%
99%
D09
97%
82%
D10
98%
99%
D11
98%
99%
D12
96%
99%
E01
98%
99%
E02
98%
99%
E03
100%
80%
E04
98%
99%
E05
100%
96%
E06
98%
99%
E07
98%
99%
E08
98%
99%
E09
98%
99%
E10
98%
99%
E11
96%
99%
E12
98%
99%
F01
92%
99%
F02
99%
99%
F03
96%
99%
F04
98%
99%
F05
96%
99%
F06
98%
99%
F07
98%
99%
F08
98%
99%
F09
98%
99%
F10
98%
99%
F11
92%
99%
F12
98%
99%
G01
98%
99%
G02
98%
99%
G03
98%
99%
G04
98%
99%
G05
96%
91%
G06
98%
99%
G07
100%
94%
G08
98%
99%
G09
92%
99%
G10
97%
100%
G11
96%
91%
HE588133.1
FJ914884.1
FJ914884.1
HE588133.1
FN185975.1
HE588133.1
HE588133.1
FJ914884.1
HF911371.1
HF911371.1
AB645751.1
HE588133.1
FN539063.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
FJ914884.1
HE588133.1
KF251162.1
HE588133.1
HE588133.1
HE588133.1
EF577236.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
KF251162.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
HE588133.1
FN908919.1
HE588133.1
GU214682.1
HE588133.1
KF251162.1
KC311519.1
FN908919.1
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Umbilicaria polyphylla
Ascomycota
Umbilicariaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Botryosphaeria dothidea
Ascomycota
Botryosphaeriaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Stereum hirsutum
Basidiomycota
Stereaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Neosetophoma samarorum
Ascomycota
Phaeosphaeriaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Mycosphaerellaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Neosetophoma samarorum
Ascomycota
Phaeosphaeriaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Neosetophoma samarorum
Ascomycota
Phaeosphaeriaceae
Sarocladium strictum
Ascomycota
Hypocreaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
66
G12
93%
98%
H01
96%
91%
H02
91%
97%
H03
100%
82%
H04
98%
99%
H05
96%
99%
H06
96%
99%
H07
98%
99%
H08
92%
99%
H09
98%
99%
H10
98%
99%
H11
98%
99%
H12
93%
98%
KJ140563.1
FN908919.1
KF251162.1
EF155488.1
HE588133.1
FJ914884.1
FJ914884.1
HE588133.1
KF251162.1
HE588133.1
HE588133.1
HE588133.1
KJ140563.1
Stereum complicatum
Basidiomycota
Stereaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Neosetophoma samarorum
Ascomycota
Phaeosphaeriaceae
Biscogniauxia nummularia
Ascomycota
Xylariaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Neosetophoma samarorum
Ascomycota
Phaeosphaeriaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Stereum complicatum
Basidiomycota
Stereaceae
Fungal Profile of CRM LB10
Well
Query
Cover
%
Identity
A02
98%
99%
A03
96%
99%
A04
98%
99%
A05
100%
99%
A06
99%
99%
A07
96%
99%
A08
98%
99%
A09
98%
99%
A10
95%
99%
A11
96%
99%
A12
98%
99%
B02
95%
99%
B03
95%
99%
B04
98%
99%
B05
96%
99%
B06
98%
99%
B07
96%
99%
B08
98%
99%
B09
100%
91%
B10
96%
99%
B11
98%
99%
Accesion #
BLAST Match
Phylum
Family
HE588133.1
FJ914884.1
HE588133.1
JF439491.1
JF439491.1
FJ914884.1
HE588133.1
HE588133.1
FJ914884.1
FJ914884.1
HE588133.1
FJ914884.1
FJ914884.1
HE588133.1
FJ914884.1
HE588133.1
FJ914884.1
HE588133.1
AF083191.1
FJ914884.1
HE588133.1
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Phialophora phaeophora
Ascomycota
Chaetosphaeriaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Ascomycota
Davidiellaceae
B12
100%
100%
HQ832794.1
Cladosporium
cladosporioides
C01
96%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
C02
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
C03
98%
99%
FJ914884.1
HE588133.1
HE588133.1
Geomyces destructans
Ascomycota
Pseudeurotiaceae
67
C04
98%
99%
C05
98%
99%
C06
95%
99%
C07
96%
99%
C08
x
C09
96%
99%
C10
98%
99%
C11
96%
99%
C12
96%
99%
D01
96%
99%
D02
98%
99%
D03
96%
99%
D04
98%
99%
D05
98%
99%
D06
95%
99%
D07
98%
99%
D08
96%
99%
D09
96%
99%
D10
100%
99%
D11
96%
99%
D12
98%
99%
E01
100%
99%
E02
97%
99%
E03
95%
99%
E04
96%
99%
E05
95%
99%
E06
95%
99%
E07
98%
99%
E08
99%
97%
E09
98%
99%
E10
98%
99%
E11
98%
99%
E12
96%
99%
F01
96%
99%
F02
95%
99%
F03
97%
99%
F04
96%
99%
F05
95%
99%
F06
98%
99%
F07
94%
99%
F08
96%
99%
F09
96%
99%
F10
98%
99%
HE588133.1
HE588133.1
FJ914884.1
FJ914884.1
FJ914884.1
HE588133.1
FJ914884.1
FJ914884.1
FJ914884.1
KF472154.1
FJ914884.1
HE588133.1
HE588133.1
FJ914884.1
HE588133.1
FJ914884.1
FJ914884.1
JF439491.1
FJ914884.1
HE588133.1
AB369919.1
HE588133.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
HE588133.1
AB369919.1
HE588133.1
HE588133.1
HE588133.1
FJ914884.1
FJ914884.1
FJ914884.1
HE588133.1
FJ914884.1
FJ914884.1
HE588133.1
FJ914884.1
FJ914884.1
FJ914884.1
HE588133.1
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Fusarium sp.
Ascomycota
Nectriaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Trichosporon asahii
Basidiomycota
Trichosporonaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Trichosporon asahii
Basidiomycota
Trichosporonaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
68
F11
96%
99%
F12
100%
99%
FJ914884.1
JF439491.1
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Cladosporium
cladosporioides
Geomyces destructans
Ascomycota
Davidiellaceae
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Fusarium oxysporum
Ascomycota
Nectriaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Ascomycota
Davidiellaceae
G01
100%
100%
HQ832794.1
G02
98%
99%
G03
98%
99%
G04
98%
99%
G05
98%
99%
G06
95%
99%
G07
98%
100%
G08
98%
99%
G09
98%
99%
G10
98%
99%
G11
96%
99%
HE588133.1
HE588133.1
HE588133.1
HE588133.1
FJ914884.1
EU839370.1
HE588133.1
HE588133.1
HE588133.1
FJ914884.1
G12
100%
100%
HQ832794.1
Cladosporium
cladosporioides
H01
98%
99%
Fusarium oxysporum
Ascomycota
Nectriaceae
H02
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H03
96%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H04
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H05
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H06
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H07
96%
99%
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
H08
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H09
99%
100%
Trichosporon asahii
Basidiomycota
Trichosporonaceae
H10
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H11
98%
99%
Geomyces destructans
Ascomycota
Pseudeurotiaceae
H12
98%
99%
EU839370.1
HE588133.1
FJ914884.1
HE588133.1
HE588133.1
HE588133.1
FJ914884.1
HE588133.1
AB369919.1
HE588133.1
HE588133.1
HE588133.1
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Well
Query
Cover
%
Identity
A02
96%
99%
A03
97%
99%
A04
96%
99%
A05
96%
99%
A06
96%
99%
A07
96%
99%
A08
100%
99%
A09
96%
9%
A10
96%
99%
A11
96%
99%
A12
96%
99%
B02
97%
99%
Fungal Profile of CRM ESF2
Accesion #
BLAST Match
Phylum
Family
FJ914884.1
JX270445.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
JF439487.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
JX270445.1
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Penicillium sp.
Ascomycota
Aspergillaceae
69
B03
100%
100%
B04
96%
99%
B05
95%
99%
B06
100%
99%
B07
96%
99%
B08
96%
99%
B09
100%
99%
B10
96%
99%
B11
96%
99%
B12
99%
99%
C01
96%
99%
C02
100%
99%
C03
95%
99%
C04
96%
99%
C05
100%
100%
C06
38%
94%
C07
100%
100%
C08
100%
99%
C09
100%
99%
C10
96%
99%
C11
96%
99%
C12
96%
99%
D01
96%
99%
D02
96%
99%
D03
100%
99%
D04
38%
95%
D05
100%
99%
D06
100%
100%
D07
95%
99%
D08
96%
99%
D09
96%
99%
D10
95%
99%
D11
96%
99%
D12
96%
99%
E01
96%
94%
E02
96%
99%
E03
95%
99%
E04
95%
99%
E05
95%
99%
E06
40%
90%
E07
100%
99%
E08
96%
99%
E09
95%
99%
JF439487.1
FJ914884.1
FJ914884.1
JF439487.1
FJ914884.1
FJ914884.1
JF439487.1
FJ914884.1
FJ914884.1
JF439487.1
FJ914884.1
JF439487.1
EU661888.1
FJ914884.1
JN713143.1
AB724312.1
JF439487.1
JF439487.1
JF439487.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
JF439487.1
AB724312.1
JF439487.1
HQ702343.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
JX270445.1
FJ914884.1
AY387141.1
JF439487.1
FJ914884.1
FJ914884.1
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Piptoporus betulinus
Basidiomycota
Fomitopsidaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Solanum lycopersicum
Solanaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Rhodotorula mucilaginosa
Basidiomycota
incertæ sedis
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
70
E10
95%
99%
E11
96%
99%
E12
96%
99%
F01
95%
99%
F02
96%
99%
F03
96%
99%
F04
95%
99%
F05
95%
99%
F06
96%
99%
F07
96%
99%
F08
96%
99%
F09
96%
99%
F10
96%
99%
F11
97%
99%
F12
96%
99%
G01
96%
99%
G02
96%
99%
G03
96%
99%
G04
96%
99%
G05
96%
99%
G06
96%
99%
G07
96%
99%
G08
99%
69%
G09
95%
99%
G10
96%
99%
G11
x
G12
100%
99%
H01
38%
95%
H02
x
H03
x
H04
100%
99%
H05
95%
99%
H06
100%
99%
H07
96%
99%
H08
100%
100%
H09
95%
99%
H10
46%
85%
H11
99%
99%
H12
100%
99%
Well
Query
Cover
%
Identity
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
JX270445.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
FJ914884.1
JF439491.1
FJ914884.1
FJ914884.1
JF439487.1
AB724312.1
JF439487.1
FJ914884.1
JF439487.1
FJ914884.1
AB369919.1
FJ914884.1
JN604461.1
JF439490.1
JF439487.1
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Trichosporon asahii
Basidiomycota
Trichosporonaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Chalara sp.
Ascomycota
Helotiaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Phylum
Family
Fungal Profile of MRC MYSO1
Accesion #
BLAST Match
71
A02
97%
93%
A03
40%
90%
A04
96%
91%
A05
99%
99%
A06
96%
100%
A07
40%
90%
A08
99%
99%
A09
100%
99%
A10
99%
99%
A11
99%
100%
A12
100%
99%
B02
96%
91%
B03
40%
90%
B04
94%
100%
B05
100%
99%
B06
99%
100%
B07
100%
99%
B08
96%
91%
B09
99%
99%
B10
12%
100%
B11
99%
100%
B12
96%
91%
C02
99%
99%
C03
99%
100%
C04
96%
91%
C05
99%
100%
C06
99%
99%
C07
99%
100%
C08
96%
91%
C09
99%
99%
C10
96%
100%
C11
100%
99%
C12
96%
99%
D01
99%
100%
D02
100%
100%
D03
99%
100%
D04
97%
99%
D05
99%
99%
D06
100%
91%
D07
99%
100%
D08
99%
100%
D09
100%
99%
HG738867.1
AY387141.1
FN908919.1
AF455525.1
AY015439.1
AY387141.1
AF455525.1
HQ607983.1
AF455525.1
AF455525.1
GU214682.1
FN908919.1
AY387141.1
AY015439.1
HQ832794.1
AF455525.1
HQ832794.1
FN908919.1
AF455525.1
KF454696.1
AF455525.1
FN908919.1
Escherichia coli
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Sporobolomyces ruberrimus
Basidiomycota
Incierta
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Sporobolomyces ruberrimus
Basidiomycota
Incierta
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Teratosphaeria zuluensis
Ascomycota
Dothideomycetes
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
C01
AF455525.1
AF455525.1
FN908919.1
AF455525.1
AF455525.1
AF455525.1
FN908919.1
AF455525.1
AY015439.1
HQ607834.1
AY015439.1
AF455525.1
HQ832794.1
AF455525.1
AF455525.1
AF455525.1
AJ301970.1
AF455525.1
AF455525.1
HQ607983.1
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Sporobolomyces ruberrimus
Basidiomycota
Incierta
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Sporobolomyces ruberrimus
Basidiomycota
Incierta
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Glomerella lagenaria
Ascomycota
Glomerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
72
FN908919.1
JF439487.1
AF455525.1
AB214657.1
AF455525.1
AF455525.1
GU214682.1
HQ832794.1
AY251074.2
HQ115724.1
HQ115724.1
D10
96%
91%
D11
99%
99%
D12
99%
100%
E01
100%
99%
E02
99%
99%
E03
99%
100%
E04
100%
99%
E05
100%
99%
E06
99%
99%
E07
100%
100%
E08
100%
100%
E09
x
E10
x
E11
40%
89%
E12
100%
99%
F01
99%
96%
F02
x
F03
97%
99%
F04
96%
91%
F05
96%
91%
F06
96%
91%
F07
100%
99%
F08
96%
91%
F09
96%
80%
JX270501.1
FN908919.1
FN908919.1
FN908919.1
GU214682.1
FN908919.1
AY015439.1
F10
x
F11
x
91%
F12
x
G01
x
G02
96%
G03
x
G04
96%
G05
x
G06
x
G07
96%
G08
x
G09
x
G10
x
G11
x
G12
x
H01
x
H02
x
H03
x
H04
48%
AY387141.1
GQ121322.2
AF455525.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Verticillium leptobactrum
Ascomycota
Plectosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Alternaria alternata
Ascomycota
Pleosporaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
X
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Sporobolomyces ruberrimus
Basidiomycota
Incierta
FN908919.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
100%
AY015439.1
Sporobolomyces ruberrimus
Basidiomycota
Incierta
100%
AY015439.1
Sporobolomyces ruberrimus
Basidiomycota
Incierta
100%
AY765415.1
Syrrhopodon banksii
plant
73
H05
x
H06
x
H07
x
H08
x
H09
x
H10
x
H11
96%
91%
H12
61%
80%
Well
Query
Cover
% Identity
Accesion #
A02
97%
99%
A03
95%
99%
A04
90%
99%
A05
95%
95%
A06
96%
99%
A07
95%
100%
A08
95%
99%
A09
92%
99%
A10
99%
99%
A11
92%
91%
A12
95%
100%
B02
94%
100%
B03
92%
99%
B04
92%
99%
B05
95%
99%
B06
93%
99%
B07
95%
99%
B08
95%
100%
B09
100%
98%
B10
97%
99%
B11
93%
99%
B12
93%
99%
C01
92%
99%
JX270404.1
AF455525.1
HE578068.1
EU497950.1
KF212290.1
HQ115724.1
KF212290.1
JX270404.1
AF455525.1
FN908919.1
HQ115724.1
AF455525.1
JX270404.1
JX270404.1
KF212290.1
JX270404.1
KF212290.1
AF455525.1
KF212290.1
JX270404.1
JX270404.1
JX270404.1
JX270404.1
AF455525.1
FN908919.1
KF212290.1
AF455525.1
AF455525.1
AF455525.1
AF455525.1
FN908919.1
FR719968.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Mastigobasidium intermedium
Basidiomycota
Leucosporidiaceae
BLAST Match
Phylum
Family
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Fungal Profile of MRC MYSO2
C02
C03
92%
91%
C04
97%
99%
C05
95%
99%
C06
95%
99%
C07
96%
99%
C08
95%
99%
Aspergillus conicus
Ascomycota
Aspergillaceae
Talaromyces rotundus
Ascomycota
Trichocomaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
74
C09
96%
99%
C10
92%
99%
C11
94%
99%
C12
95%
100%
D01
95%
100%
D02
95%
100%
D03
96%
100%
D04
93%
99%
D05
96%
99%
D06
93%
99%
D07
95%
99%
D08
95%
99%
D09
95%
100%
D10
93%
99%
D11
96%
99%
D12
99%
99%
E01
95%
99%
E02
93%
91%
E03
95%
99%
E04
95%
99%
E05
92%
99%
E06
39%
90%
E07
100%
99%
E08
93%
99%
E09
100%
99%
E10
93%
99%
E11
95%
100%
E12
63%
98%
F01
60%
100%
F02
100%
99%
F03
93%
91%
F04
100%
99%
F05
94%
99%
F06
81%
75%
F07
96%
99%
F08
95%
100%
F09
95%
91%
F10
93%
99%
F11
96%
99%
F12
92%
99%
G01
95%
99%
G02
G03
95%
100%
AF455525.1
JX270404.1
KF212291.1
AF455525.1
AF455525.1
AF455525.1
AF455525.1
JX270404.1
KF212290.1
JX270404.1
AF455525.1
KF212290.1
AF455525.1
JX270404.1
KF212290.1
AF455525.1
AF455525.1
FN908919.1
KF212290.1
AF455525.1
JX270404.1
AY387141.1
AF455525.1
JX270404.1
AF455525.1
JX270404.1
AF455525.1
KC920582.1
KC920582.1
AF455525.1
FN908919.1
KF212290.1
JX270404.1
FJ896135.1
KF212290.1
AF455525.1
FN908919.1
JX270404.1
AF455525.1
JX270404.1
KF212290.1
AF455525.1
AF455525.1
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Exobasidium arescens
Basidiomycota
Exobasidiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
75
G04
93%
99%
G05
93%
99%
G06
72%
99%
G07
96%
99%
G08
96%
99%
G09
92%
82%
G10
96%
99%
G11
96%
100%
G12
96%
99%
H01
93%
99%
H02
97%
99%
H03
32%
89%
H04
91%
82%
H05
90%
99%
H06
93%
99%
H07
94%
100%
H08
90%
99%
H09
95%
100%
H10
95%
99%
H11
95%
100%
H12
95%
100%
JX270404.1
JX270404.1
KC920582.1
KF212290.1
KF212290.1
DQ682574.1
KF212290.1
AY251074.2
EF555097.3
JX270404.1
JX270404.1
EU486992.1
DQ682574.1
JX270404.1
JX270404.1
JQ768326.1
HE578068.1
AF455525.1
AF455525.1
AF455525.1
AF455525.1
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Exobasidiomycetidae sp.
Basidiomycota
Exobasidiomycetidae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Paecilomyces hepiali
Ascomycota
Clavicipitaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Golovinomyces cichoracearum
Ascomycota
Erysiphaceae
Exobasidiomycetidae sp.
Basidiomycota
Exobasidiomycetidae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Aspergillus conicus
Ascomycota
Aspergillaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Fungal Profile of MRC MYSO3
Well
Query
Cover
% Identity
Accesion #
BLAST Match
Phylum
Family
A02
100%
99%
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
A03
96%
91%
A04
100%
99%
A05
100%
99%
A06
100%
99%
A07
99%
99%
A08
99%
99%
A09
99%
100%
A10
100%
99%
A11
99%
100%
A12
100%
91%
B02
100%
99%
B03
99%
97%
B04
99%
99%
B05
100%
99%
B06
99%
99%
B07
99%
99%
HQ607983.1
FN908919.1
HQ607983.1
HQ607983.1
HQ607983.1
AF455525.1
AF455525.1
AF455525.1
HQ607983.1
AF455525.1
HQ607983.1
HQ607983.1
HQ607983.1
AF455525.1
HQ607983.1
AF455525.1
AF455525.1
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
76
B08
100%
99%
B09
99%
99%
B10
99%
100%
B11
99%
99%
B12
100%
99%
C01
99%
99%
C02
100%
99%
C03
100%
99%
C04
100%
99%
C05
99%
99%
C06
100%
96%
C07
100%
99%
C08
100%
99%
C09
100%
99%
C10
99%
99%
C11
100%
99%
C12
100%
99%
D01
100%
99%
D02
96%
99%
D03
100%
99%
D04
98%
99%
D05
96%
91%
D06
100%
99%
D07
100%
91%
D08
100%
99%
D09
40%
89%
D10
100%
99%
D11
100%
99%
D12
100%
100%
E01
97%
99%
E02
100%
99%
E03
100%
99%
E04
99%
100%
E05
100%
99%
E06
100%
99%
E07
100%
99%
E08
99%
99%
E09
99%
100%
E10
99%
100%
E11
100%
99%
E12
100%
99%
F01
100%
99%
F02
100%
99%
HQ607983.1
AF455525.1
AF455525.1
AF455525.1
HQ607983.1
JN617677.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
FJ914884.1
HQ607983.1
KC785547.1
FN908919.1
HQ607983.1
GU214682.1
HQ607983.1
AY387141.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
AF455525.1
HQ607983.1
HQ607983.1
HQ607983.1
AF455525.1
AF455525.1
AF455525.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Penicillium pancosmium
Ascomycota
Aspergillaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Leucosporidium drummii
Basidiomycota
Leucosporidiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Malassezia pachydermatis
Basidiomycota
Malasseziaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
77
F03
100%
99%
F04
100%
99%
F05
100%
99%
F06
100%
100%
F07
99%
100%
F08
100%
99%
F09
100%
100%
F10
100%
100%
F11
100%
99%
F12
100%
99%
G01
99%
99%
G02
100%
99%
G03
100%
99%
G04
99%
100%
G05
99%
100%
G06
99%
100%
G07
99%
100%
G08
100%
99%
G09
100%
99%
G10
100%
99%
G11
100%
99%
G12
99%
99%
H01
100%
99%
H02
99%
99%
H03
100%
100%
H04
100%
99%
H05
100%
99%
H06
100%
99%
H07
99%
99%
H08
100%
99%
H09
100%
99%
H10
97%
99%
H11
100%
99%
H12
100%
99%
Well
Query
Cover
%
Identity
A02
93%
99%
A03
96%
99%
A04
96%
99%
A05
96%
100%
HQ607983.1
HQ607983.1
HQ115671.1
HQ607834.1
AF455525.1
HQ607983.1
HQ115724.1
HQ607983.1
GU214682.1
HQ607983.1
HQ607983.1
GU214682.1
HQ607983.1
AF455525.1
AF455525.1
AF455525.1
AF455525.1
HQ607983.1
HQ607983.1
HQ607983.1
HQ607983.1
AF455525.1
HQ607983.1
AF455525.1
GU214682.1
HQ607983.1
GU214682.1
GU214682.1
AF455535.1
HQ607983.1
HQ607983.1
JX270445.1
HQ607983.1
HQ115724.1
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Trichoderma atroviride
Ascomycota
Hypocreaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Isaria farinosa
Ascomycota
Cordycipitaceae
Phylum
Family
Fungal Profile of MRC COTO6
Accesion #
JX270445.1
KC785585.1
JN942650.1
DQ339561.1
BLAST Match
Penicillium sp.
Ascomycota
Aspergillaceae
Uncultured Malassezia
Basidiomycota
Malasseziaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Penicillium concentricum
Ascomycota
Aspergillaceae
78
A06
94%
98%
A07
96%
99%
A08
95%
99%
A09
94%
99%
A10
93%
98%
A11
86%
99%
A12
x
B02
93%
99%
B03
94%
97%
B04
93%
98%
B05
96%
99%
B06
96%
99%
B07
100%
99%
B08
100%
99%
B09
93%
100%
B10
96%
98%
B11
95%
99%
B12
96%
99%
C01
95%
99%
C02
96%
97%
C03
96%
98%
C04
x
JX270427.1
JN942650.1
DQ339561.1
JX270593.1
JX270427.1
JN206049.1
JX270445.1
JX270427.1
JX270427.1
JN942650.1
KC785585.1
AB214657.1
AB214657.1
JX270470.1
NR_077068.1
DQ339561.1
JN942650.1
AB369919.1
NR_077068.1
NR_077068.1
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Penicillium concentricum
Ascomycota
Aspergillaceae
Gymnoascus sp.
Ascomycota
Gymnoascaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Mucor flavus
Zygomycota
Mucoraceae
Penicillium sp.
Ascomycota
Aspergillaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Uncultured Malassezia
Basidiomycota
Malasseziaceae
Verticillium leptobactrum
Ascomycota
Plectosphaerellaceae
Verticillium leptobactrum
Ascomycota
Plectosphaerellaceae
Kernia sp.2
Ascomycota
Microascaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Penicillium concentricum
Ascomycota
Aspergillaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Trichosporon asahii
Basidiomycota
Trichosporonaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Alternaria citri
Ascomycota
Pleosporaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
JN942650.1
NR_077068.1
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
C05
x
C06
100%
99%
C07
97%
98%
C08
x
C09
97%
99%
C10
97%
98%
C11
x
C12
93%
99%
D01
96%
99%
D02
96%
99%
D03
96%
99%
D04
96%
99%
D05
95%
99%
D06
97%
99%
D07
100%
99%
D08
100%
99%
D09
94%
99%
D10
95%
99%
D11
97%
99%
JX230994.1
KF542551.1
AY251074.2
HQ607834.1
JN942650.1
JQ776537.1
HQ607983.1
HQ607983.1
JN942650.1
AY251074.2
JN942650.1
JN942650.1
D12
x
E01
97%
94%
JQ768326.1
AY154705.1
NR_077068.1
Alternaria brassicicola
Ascomycota
Pleosporaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
79
E02
91%
99%
E03
95%
99%
E04
95%
99%
E05
93%
99%
E06
89%
99%
E07
100%
98%
E08
94%
99%
E09
94%
92%
E10
95%
99%
E11
96%
99%
E12
x
F01
94%
98%
F02
97%
99%
F03
96%
92%
F04
92%
99%
F05
97%
99%
F06
100%
99%
F07
97%
99%
F08
100%
99%
F09
94%
99%
F10
85%
97%
F11
95%
98%
JN942650.1
JQ776537.1
JQ776537.1
JX270445.1
JQ776537.1
NR_077068.1
JQ776537.1
JN859359.1
AY154705.1
HE588133.1
F12
93%
99%
G01
97%
92%
G02
100%
99%
G03
100%
99%
G04
93%
92%
G05
91%
99%
G06
97%
99%
G07
98%
98%
G08
60%
100%
AB470827.1
JN942650.1
GU566291.1
JN942650.1
JN942650.1
JN942650.1
JX270470.1
JN942650.1
JX270445.1
AF291276.1
JX270427.1
JX270470.1
AJ007846.1
JN942650.1
JN942650.1
JN859359.1
JQ776537.1
JN942650.1
JX270427.1
KF221212.1
G09
x
G10
96%
98%
G11
x
G12
97%
99%
H01
96%
99%
H02
100%
99%
H03
97%
98%
H04
92%
98%
H05
x
H06
95%
99%
H07
100%
99%
H08
97%
92%
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Pleosporales sp.
Ascomycota
Alternaria citri
Ascomycota
Pleosporaceae
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Ascomycota
Debaryomycetaceae
Zygomycota
Mortierellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Geomyces destructans
Cytospora mali
Debaryomyces maramus
Paecilomyces inflatus
Kernia sp.
Debaryomyces maramus
Penicillium sp.
Exidia recisa
Mortierella sp.
Kernia sp.
Chrysosporium vespertilium
Pleosporales sp.
Ascomycota
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Penicillium sclerotiorum
Ascomycota
Aspergillaceae
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
JN942650.1
JN942650.1
JN942650.1
NR_077068.1
NR_077068.1
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
JQ776537.1
GU797138.1
AJ007846.1
Cladosporium sphaerospermum
Ascomycota
Cladosporiaceae
Alternaria alternata
Ascomycota
Pleosporaceae
Chrysosporium vespertilium
Ascomycota
Cladosporiaceae
80
H09
97%
99%
H10
97%
100%
H11
95%
97%
H12
97%
98%
Well
Query
Cover
%
Identity
A02
100%
99%
A03
97%
99%
A04
93%
98%
A05
95%
98%
A06
94%
97%
A07
96%
99%
A08
94%
99%
A09
91%
99%
A10
89%
99%
A11
99%
99%
A12
95%
98%
B02
85%
99%
B03
95%
98%
B04
93%
98%
B05
95%
96%
B06
95%
98%
B07
98%
98%
B08
92%
98%
B09
88%
99%
B10
91%
98%
B11
95%
97%
B12
93%
98%
C01
86%
99%
C02
96%
93%
C03
85%
99%
C04
90%
98%
C05
88%
99%
C06
93%
98%
C07
86%
99%
C08
94%
97%
C09
93%
99%
C10
96%
96%
C11
92%
92%
C12
95%
99%
JN942650.1
DQ339561.1
JX270427.1
NR_077068.1
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Penicillium concentricum
Ascomycota
Aspergillaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Fungal Profile of MRC COTO7
Accesion #
BLAST Match
Phylum
Family
HQ832794.1
JX270501.1
JX270427.1
NR_077068.1
JX270427.1
JN942650.1
JN942650.1
EU181451.1
JX270467.1
JN942650.1
NR_077068.1
JN206049.1
JN942650.1
AB470827.1
HQ608097.1
JX270427.1
NR_077068.1
JX270427.1
JX270467.1
AB470827.1
NR_077068.1
AB470827.1
JX270467.1
JX270406.1
JN206049.1
JX270427.1
JX270427.1
JX270427.1
JQ761667.1
NR_077068.1
JN942650.1
HQ608097.1
EU343240.1
JN942650.1
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Mortierella sp. 3
Zygomycota
Mortierellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Arthroderma quadrifidum
Ascomycota
Arthrodermataceae
Helicostylum sp.
Zygomycota
Mucoraceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mucor flavus
Zygomycota
Mucoraceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cytospora mali
Ascomycota
Valsaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Helicostylum sp.
Zygomycota
Mucoraceae
Cytospora mali
Ascomycota
Valsaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Cytospora mali
Ascomycota
Valsaceae
Helicostylum sp.
Zygomycota
Mucoraceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mucor flavus
Zygomycota
Mucoraceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Dothideomycetes sp.
Ascomycota
Dothideomycetes
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Mycosphaerella punctiformis
Ascomycota
Mycosphaerellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
81
D01
96%
97%
D02
86%
99%
D03
96%
97%
D04
98%
99%
D05
100%
97%
D06
92%
99%
D07
97%
99%
D08
99%
96%
D09
98%
80%
D10
96%
91%
D11
95%
98%
D12
92%
96%
E01
96%
99%
E02
94%
97%
E03
94%
98%
E04
84%
99%
E05
94%
98%
E06
87%
99%
E07
97%
99%
E08
94%
98%
E10
93%
98%
E11
89%
88%
E12
94%
99%
F01
96%
96%
F02
95%
99%
F03
94%
99%
F04
94%
98%
F05
88%
99%
F06
86%
99%
F07
97%
98%
F09
95%
99%
F10
72%
96%
F11
83%
85%
F12
95%
97%
G01
86%
99%
G02
87%
99%
G03
97%
98%
G04
96%
99%
G05
97%
98%
G06
93%
99%
G07
92%
98%
NR_077068.1
JX270467.1
NR_077068.1
HE588133.1
HQ608097.1
JX270393.1
KF212294.1
KF212294.1
FJ755263.1
HQ608097.1
JN942672.1
HQ608097.1
AB214657.1
JX270427.1
JX270427.1
JN206049.1
JX270427.1
JN206049.1
JN942650.1
JX270427.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Helicostylum sp.
Zygomycota
Mucoraceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Hyalodendriella betulae
Ascomycota
Davidiellaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Verticillium leptobactrum
Ascomycota
Clavicipitaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mucor flavus
Zygomycota
Mucoraceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mucor flavus
Zygomycota
Mucoraceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Helicostylum sp.
Zygomycota
Mucoraceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
E09
JX270427.1
JX270467.1
JX270501.1
JX500428.1
HE588133.1
AB470827.1
JX270427.1
JQ761667.1
JN206049.1
AB470827.1
Cytospora mali
Ascomycota
Valsaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Dothideomycetes sp.
Ascomycota
Mucor flavus
Zygomycota
Mucoraceae
Cytospora mali
Ascomycota
Valsaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Cytospora mali
Ascomycota
Valsaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mucor flavus
Zygomycota
Mucoraceae
Dothideomycetes sp.
Ascomycota
F08
HE588133.1
AB470827.1
JX270427.1
JX270478.1
JN206049.1
JQ761667.1
JX270539.1
AB214657.1
NR_077068.1
JX270470.1
AB470827.1
Oidiodendron sp.
Ascomycota
Myxotrichaceae
Verticillium leptobactrum
Ascomycota
Clavicipitaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Kernia sp.
Ascomycota
Microascaceae
Cytospora mali
Ascomycota
Valsaceae
82
G08
93%
98%
G09
94%
98%
G10
91%
91%
G11
77%
99%
G12
97%
98%
H01
95%
96%
H02
94%
99%
H03
97%
99%
H04
95%
98%
H05
95%
98%
H06
84%
99%
H07
84%
99%
H08
94%
98%
H09
93%
99%
H10
95%
99%
H11
100%
99%
H12
95%
96%
JX270427.1
JX270427.1
JN859359.1
KC800713.1
NR_077068.1
HQ608097.1
JX270470.1
JN942650.1
JX270427.1
JX270427.1
FM202495.1
FM202495.1
AB470827.1
JX270470.1
HE588133.1
JN942650.1
JX500428.1
Mortierella sp.
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Pleosporales sp.
Ascomycota
Calcarisporium sp.
Ascomycota
Ophiostomataceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Kernia sp.
Ascomycota
Microascaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Mortierella sp.
Zygomycota
Mortierellaceae
Physisporinus vitreus
Basidiomycota
Polyporaceae
Physisporinus vitreus
Basidiomycota
Polyporaceae
Cytospora mali
Ascomycota
Valsaceae
Kernia sp.
Ascomycota
Microascaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Fungal Profile of MRC COTO8
Well
Query
Cover
%
Identity
A02
96%
99%
A03
100%
93%
A04
86%
97%
A05
86%
97%
A06
91%
99%
A07
94%
91%
A08
95%
98%
A09
100%
99%
A10
100%
98%
A11
98%
97%
A12
99%
98%
B02
100%
98%
B03
95%
97%
B04
93%
99%
B05
97%
99%
B06
97%
100%
B07
95%
98%
B08
46%
97%
B09
96%
96%
B10
94%
100%
B11
99%
97%
B12
95%
98%
Accesion #
BLAST Match
Phylum
Family
EU400587.1
HG738867.1
AF291276.1
AF291276.1
JX270470.1
JX270427.1
JX270427.1
KC785585.1
JX270427.1
JX270427.1
NR_077068.1
NR_077068.1
JX270427.1
FJ914884.1
EU400587.1
JX270470.1
JX270427.1
HG738867.1
JX500428.1
JX270470.1
HE588133.1
AB470827.1
Malassezia restricta
Basidiomycota
Malasseziaceae
Exidia recisa
Basidiomycota
Exidiaceae
Exidia recisa
Basidiomycota
Exidiaceae
Kernia sp.
Ascomycota
Microascaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Malassezia
Basidiomycota
Mortierella sp.3
Zygomycota
Escherichia coli
Mortierellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Malassezia restricta
Basidiomycota
Malasseziaceae
Kernia sp.
Ascomycota
Microascaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Kernia sp.
Ascomycota
Microascaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Cytospora mali
Ascomycota
Valsaceae
E. coli
83
C01
97%
99%
C02
100%
99%
C03
96%
99%
C04
98%
98%
C05
86%
97%
C06
100%
98%
C07
95%
98%
C08
98%
99%
C09
96%
96%
C10
97%
99%
C11
96%
96%
C12
96%
99%
D01
94%
98%
D02
91%
99%
D03
91%
99%
D04
97%
99%
D05
97%
99%
D06
94%
98%
D07
97%
97%
D08
92%
99%
D09
94%
99%
D10
97%
100%
D11
96%
98%
D12
94%
96%
E01
95%
99%
E02
92%
99%
E03
97%
99%
E04
93%
97%
E05
89%
99%
E06
92%
99%
E07
96%
99%
E08
98%
99%
E09
96%
99%
E10
95%
99%
E11
94%
98%
E12
94%
99%
F01
97%
100%
F02
96%
99%
F03
93%
99%
F04
95%
98%
F05
86%
97%
F06
94%
99%
F07
94%
99%
JN942650.1
JN942650.1
FJ914884.1
JX270427.1
AF291276.1
NR_077068.1
JX270427.1
JN942650.1
JX500428.1
JN942650.1
JX500428.1
JN942650.1
JX270427.1
JQ776537.1
JQ776537.1
JN942650.1
JN942650.1
NR_077068.1
NR_077068.1
JQ776537.1
JX270501.1
KF542551.1
NR_077068.1
HQ608097.1
KC785585.1
FJ914884.1
JN942650.1
JQ768326.1
JQ776537.1
AJ437693.1
FJ914884.1
AY251074.2
JN942650.1
JQ776537.1
AB470827.1
JX270501.1
AY154705.1
AY251074.2
JX270470.1
JX270427.1
AF291276.1
JX270470.1
JX270445.1
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Sporidiobolus salmonicolor
Basidiomycota
Microbotryomycetes
Mortierella sp.3
Zygomycota
Mortierellaceae
Exidia recisa
Basidiomycota
Exidiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Alternaria brassicicola
Ascomycota
Pleosporaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.4
Zygomycota
Mortierellaceae
Uncultured Malassezia
Basidiomycota
Malasseziaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Malassezia globosa
Basidiomycota
Malasseziaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Cytospora mali
Ascomycota
Valsaceae
Geomyces sp.
Ascomycota
Pseudeurotiaceae
Alternaria citri
Ascomycota
Pleosporaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
84
Kernia sp. 2
Ascomycota
Microascaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Exidia recisa
Basidiomycota
Exidiaceae
Kernia sp.
Ascomycota
Microascaceae
Penicillium sp.
Ascomycota
Aspergillaceae
F08
94%
99%
F09
94%
99%
F10
96%
99%
F11
100%
99%
F12
91%
98%
G01
96%
97%
G02
94%
98%
G03
97%
99%
G04
97%
99%
G05
95%
99%
G06
90%
99%
G07
91%
100%
G08
95%
96%
G09
96%
96%
G10
96%
96%
G11
73%
99%
G12
97%
98%
H01
51%
84%
H02
89%
90%
H03
94%
98%
H04
97%
92%
H05
92%
99%
H06
96%
99%
H07
91%
98%
H08
96%
99%
H09
x
H10
100%
100%
H11
100%
99%
H12
97%
96%
JX270445.1
JX270445.1
HE588133.1
JX270470.1
JX270427.1
NR_077068.1
JX270427.1
JN942650.1
EU400587.1
JX270593.1
JX270470.1
JX270593.1
JX500428.1
JX500428.1
JX500428.1
KC800713.1
NR_077068.1
KF251182.1
KF251182.1
JX270427.1
AJ007846.1
JQ776537.1
AB214657.1
JX270427.1
HE588133.1
HQ832794.1
JN942650.1
JX500428.1
Penicillium sp.
Ascomycota
Aspergillaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Kernia sp. 2
Ascomycota
Microascaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Malassezia restricta
Basidiomycota
Malasseziaceae
Gymnoascus sp.
Ascomycota
Gymnoascaceae
Kernia sp.
Ascomycota
Microascaceae
Gymnoascus sp.
Ascomycota
Gymnoascaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Calcarisporium sp.
Ascomycota
Davidiellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Phaeosphaeria caricicola
Ascomycota
Phaeosphaeriaceae
Phaeosphaeria caricicola
Ascomycota
Phaeosphaeriaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Chrysosporium vespertilium
Ascomycota
Onygenaceae
Cladosporium sphaerospermum
Ascomycota
Davidiellaceae
Verticillium leptobactrum
Ascomycota
Plectosphaerellaceae
Mortierella sp.3
Zygomycota
Mortierellaceae
Geomyces destructans
Ascomycota
Pseudeurotiaceae
Cladosporium cladosporioides
Ascomycota
Davidiellaceae
Debaryomyces maramus
Ascomycota
Debaryomycetaceae
Lecanicillium fungicola
Ascomycota
Cordycipitaceae
Fungal Profile of MRC COTO9
Well
Query
Cover
%
Identity
A02
93%
99%
A03
100%
99%
A04
100%
99%
A05
98%
99%
A06
100%
99%
A07
93%
99%
A08
100%
99%
A09
100%
99%
A10
100%
99%
Accesion #
BLAST Match
Phylum
Family
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
85
A11
93%
99%
A12
93%
99%
B02
93%
99%
B03
100%
98%
B04
100%
99%
B05
100%
98%
B06
100%
99%
B07
100%
98%
B08
93%
99%
B09
100%
99%
B10
100%
98%
B11
93%
99%
B12
100%
98%
C01
93%
99%
C02
100%
99%
C03
93%
99%
C04
100%
98%
C05
100%
99%
C06
100%
98%
C07
100%
98%
C08
93%
99%
C09
93%
99%
C10
100%
99%
C11
100%
98%
C12
100%
99%
D01
100%
98%
D02
100%
99%
D03
100%
99%
D04
100%
99%
D05
98%
99%
D06
100%
99%
D07
93%
99%
D08
93%
99%
D09
93%
99%
D10
93%
99%
D11
100%
99%
D12
100%
99%
E01
100%
98%
E02
100%
99%
E03
93%
99%
E04
100%
99%
E05
98%
99%
E06
98%
99%
KJ140584.1
KJ140584.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
KJ140584.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
KJ140584.1
KJ140584.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
86
E07
93%
99%
E08
93%
99%
E09
98%
98%
E10
100%
99%
E11
100%
99%
E12
100%
99%
F01
98%
99%
F02
93%
99%
F03
94%
99%
F04
93%
99%
F05
93%
99%
F06
98%
99%
F07
98%
99%
F08
98%
99%
F09
93%
99%
F10
98%
99%
F11
98%
98%
F12
100%
99%
G01
98%
99%
G02
98%
98%
G03
98%
99%
G04
93%
99%
G05
100%
98%
G06
98%
99%
G07
98%
98%
G08
98%
98%
G09
98%
99%
G10
93%
99%
G11
98%
99%
G12
98%
99%
H01
98%
99%
H02
93%
99%
H03
93%
99%
H04
98%
99%
H05
100%
99%
H06
98%
99%
H07
98%
99%
H08
98%
99%
H09
98%
99%
H10
98%
99%
H11
98%
99%
H12
93%
99%
KJ140584.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
KJ140584.1
KJ140584.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
87
Fungal Profile of SHC COTO7
Well
Query
Cover
%
Identity
A02
100%
98%
A03
100%
98%
A04
100%
97%
A05
100%
98%
A06
100%
98%
A07
100%
98%
A08
100%
98%
A09
100%
98%
A10
100%
98%
A11
100%
97%
A12
100%
98%
B02
100%
98%
B03
100%
97%
B04
100%
98%
B05
100%
98%
B06
100%
98%
B07
100%
98%
B08
100%
98%
B09
100%
98%
B10
100%
97%
B11
100%
98%
B12
100%
98%
C01
100%
98%
C02
100%
98%
C03
100%
98%
C04
100%
98%
C05
100%
98%
C06
100%
98%
C07
100%
98%
C08
100%
98%
C09
100%
98%
C10
100%
98%
C11
100%
98%
C12
100%
98%
D01
100%
98%
D02
100%
97%
D03
100%
98%
D04
100%
98%
Accession #
BLAST Match
Phylum
Family
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
88
D05
100%
98%
D06
100%
98%
D07
100%
98%
D08
100%
98%
D09
100%
98%
D10
100%
98%
D11
100%
98%
D12
100%
98%
E01
100%
98%
E02
100%
98%
E03
100%
98%
E04
100%
98%
E05
100%
98%
E06
100%
98%
E07
100%
98%
E08
100%
98%
E09
100%
98%
E10
100%
98%
E11
100%
98%
E12
100%
98%
F01
100%
98%
F02
100%
98%
F03
100%
98%
F04
100%
98%
F05
100%
98%
F06
100%
98%
F07
100%
98%
F08
100%
98%
F09
100%
98%
F10
100%
98%
F11
100%
97%
F12
100%
98%
G01
100%
98%
G02
100%
98%
G03
100%
98%
G04
100%
98%
G05
100%
98%
G06
100%
99%
G07
100%
98%
G08
100%
99%
G09
100%
98%
G10
100%
98%
G11
100%
97%
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
89
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
98%
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
96%
NR_077068.2
Debaryomyces udenii
Ascomycota
Microascaceae
Ascomycota
Debaryomycetaceae
G12
100%
98%
H01
100%
H02
98%
H03
94%
98%
NR_077068.3
Debaryomyces udenii
H04
83%
98%
NR_077068.4
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
H05
94%
98%
NR_077068.5
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
H06
82%
98%
NR_077068.6
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Ascomycota
Debaryomycetaceae
H07
83%
98%
NR_077068.1
Debaryomyces udenii
H08
83%
98%
NR_077068.2
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
H09
83%
98%
NR_077068.3
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
H10
83%
98%
NR_077068.4
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Ascomycota
Debaryomycetaceae
Ascomycota
Debaryomycetaceae
H11
82%
98%
NR_077068.5
Debaryomyces udenii
H12
83%
98%
NR_077068.6
Debaryomyces udenii
Fungal Profile of SHC COTO8
Well
Query
Cover
%
Identity
A02
96%
97%
A03
96%
98%
A04
96%
98%
A05
96%
98%
A06
100%
97%
A07
96%
98%
A08
96%
98%
A09
98%
98%
A10
96%
98%
A11
97%
98%
A12
96%
98%
B02
96%
98%
B03
96%
98%
B04
96%
98%
B05
96%
98%
B06
96%
97%
B07
98%
97%
B08
95%
98%
B09
89%
40%
B10
97%
98%
B11
40%
89%
B12
97%
98%
C01
95%
98%
C02
100%
98%
C03
42%
89%
C04
96%
98%
Accesion #
BLAST Match
Phylum
Family
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
AB769444.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
90
C05
96%
98%
C06
100%
98%
C07
40%
89%
C08
94%
99%
C09
96%
98%
C10
96%
98%
C11
96%
97%
C12
93%
98%
D01
96%
98%
D02
96%
98%
D03
96%
98%
D04
96%
98%
D05
96%
98%
D06
96%
97%
D07
42%
89%
D08
40%
89%
D09
97%
98%
D10
89%
40%
D11
96%
98%
D12
97%
98%
E01
96%
97%
E02
100%
98%
E03
97%
98%
E04
100%
99%
E05
100%
98%
E06
97%
98%
E07
97%
98%
E08
40%
89%
E09
96%
98%
E10
96%
98%
E11
40%
89%
E12
40%
89%
F01
96%
98%
F02
96%
97%
F03
100%
98%
F04
96%
97%
F05
97%
98%
F06
100%
98%
F07
97%
98%
F08
97%
97%
F09
96%
97%
F10
96%
98%
F11
96%
98%
NR_077068.1
NR_077068.1
AB769444.1
FJ914884.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
AB769444.1
NR_077068.1
AB769444.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
JX230994.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
NR_077068.1
AB769444.1
AB769444.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Sporidiobolus salmonicolor
Basidiomycota
Sporidiobolaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
91
F12
96%
97%
G01
96%
98%
G02
42%
89%
G03
90%
99%
G04
98%
98%
G05
97%
98%
G06
98%
98%
G07
97%
98%
G08
97%
98%
G09
93%
99%
G10
97%
98%
G11
97%
98%
G12
96%
98%
H01
97%
98%
H02
100%
98%
H03
97%
98%
H04
100%
98%
H05
97%
98%
H06
100%
98%
H07
97%
98%
H08
97%
98%
H09
100%
98%
H10
41%
89%
H11
97%
97%
H12
41%
89%
NR_077068.1
NR_077068.1
AB769444.1
HE578068.1
NR_077068.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
JX270445.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB214657.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
AB769444.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Aspergillus conicus
Ascomycota
Aspergillaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Penicillium sp.1
Ascomycota
Aspergillaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Verticillium leptobactrum
Ascomycota
Clavicipitaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Fungal Profile of SHC COTO9
Well
Query
Cover
%
Identity
A02
100%
98%
A03
100%
97%
A04
99%
95%
A05
100%
98%
A06
100%
97%
A07
99%
95%
A08
100%
97%
A09
100%
98%
A10
100%
98%
A11
100%
97%
A12
100%
98%
B02
100%
B03
X
B04
100%
Accession #
BLAST Match
Phylum
Family
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
98%
NR_077068.1
NR_077068.1
FJ536210.1
NR_077068.1
NR_077068.1
FJ536210.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
98%
NR_077068.1
Microdochium sp.
Ascomycota
Hyponectriaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Microdochium sp.
Ascomycota
Hyponectriaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
92
B05
100%
97%
B06
100%
98%
B07
100%
98%
B08
42%
89%
B09
x
B10
100%
98%
B11
100%
98%
B12
100%
98%
C01
100%
98%
C02
100%
98%
C03
91%
99%
C04
100%
98%
C05
100%
98%
C06
100%
98%
C07
100%
98%
C08
100%
97%
C09
100%
99%
C10
100%
97%
C11
100%
98%
C12
100%
98%
D01
100%
98%
D02
100%
98%
D03
100%
98%
D04
100%
98%
D05
99%
97%
D06
100%
97%
D07
100%
97%
D08
100%
91%
D09
100%
98%
D10
100%
98%
D11
100%
98%
D12
100%
98%
E01
42%
89%
E02
100%
97%
E03
100%
97%
E04
42%
89%
E05
100%
98%
E06
100%
97%
E07
100%
99%
E08
100%
99%
E09
100%
97%
E10
67%
96%
E11
100%
98%
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
KJ140584.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
GU214682.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB220032.1
FJ536210.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
NR_077068.1
JN942672.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Stereum complicatum
Basidiomycota
Stereaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Pseudocercosporella fraxini
Ascomycota
Mycosphaerellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Candida psychrophila
Ascomycota
Saccharomycetaceae
Microdochium sp.
Ascomycota
Hyponectriaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
93
E12
100%
98%
F01
100%
98%
F02
100%
97%
F03
100%
98%
F04
100%
98%
F05
93%
99%
F06
93%
99%
F07
100%
98%
F08
100%
98%
F09
100%
98%
F10
100%
98%
F11
42%
89%
F12
100%
98%
G01
100%
97%
G02
100%
98%
G03
100%
98%
G04
100%
98%
G05
100%
98%
G06
100%
98%
G07
97%
99%
G08
100%
97%
G09
100%
98%
G10
100%
98%
G11
97%
99%
G12
97%
99%
H01
100%
98%
H02
99%
95%
H03
100%
98%
H04
100%
98%
H05
100%
98%
H06
100%
98%
H07
100%
98%
H08
100%
98%
H09
100%
98%
H10
100%
98%
H11
42%
89%
H12
100%
98%
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
KJ140584.1
KJ140584.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
JX270445.1
NR_077068.1
NR_077068.1
NR_077068.1
JX270445.1
JX270445.1
NR_077068.1
FJ536210.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB769444.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Penicillium sp.
Ascomycota
Aspergillaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Microdochium sp.
Ascomycota
Hyponectriaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Golovinomyces sp.
Ascomycota
Erysiphaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Fungal Profile of SHC COTO10
Well
Query
Cover
A02
X
A03
100%
%
Identity
Accesion #
BLAST Match
Phylum
Family
98%
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
94
A04
100%
97%
A05
100%
98%
A06
100%
98%
A07
100%
98%
A08
100%
98%
A09
100%
98%
A10
100%
98%
A11
100%
98%
A12
100%
98%
B02
100%
98%
B03
100%
98%
B04
100%
98%
B05
100%
98%
B06
100%
98%
B07
100%
98%
B08
100%
98%
B09
100%
98%
B10
100%
98%
B11
100%
98%
B12
100%
98%
C01
100%
98%
C02
100%
98%
C03
100%
98%
C04
100%
98%
C05
100%
98%
C06
100%
98%
C07
100%
97%
C08
100%
98%
C09
100%
98%
C10
100%
98%
C11
100%
98%
C12
100%
98%
D01
100%
97%
D02
100%
98%
D03
100%
98%
D04
100%
97%
D05
100%
97%
D06
100%
97%
D07
100%
97%
D08
100%
98%
D09
100%
98%
D10
100%
98%
D11
100%
98%
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
JQ912667.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces hansenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
95
D12
100%
97%
E01
100%
97%
E02
100%
97%
E03
100%
98%
E04
100%
98%
E05
100%
98%
E06
100%
98%
E07
100%
98%
E08
100%
98%
E09
100%
98%
E10
100%
98%
E11
100%
98%
E12
100%
98%
F01
100%
98%
F02
100%
98%
F03
98%
98%
F04
100%
98%
F05
98%
99%
F06
98%
98%
F07
100%
98%
F08
100%
99%
F09
98%
99%
F10
98%
99%
F11
98%
99%
F12
98%
99%
G01
98%
99%
G02
100%
99%
G03
100%
99%
G04
100%
99%
G05
93%
99%
G06
98%
99%
G07
100%
98%
G08
98%
98%
G09
98%
99%
G10
98%
99%
G11
100%
98%
G12
100%
97%
H01
100%
98%
H02
100%
98%
H03
100%
98%
H04
100%
98%
H05
100%
97%
H06
100%
98%
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
KJ140584.1
KJ140584.1
AY089730.1
KJ140584.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
AY089730.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum complicatum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Stereum sanguinolentum
Basidiomycota
Stereaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
96
H07
100%
98%
H08
100%
97%
H09
100%
98%
H10
100%
98%
H11
100%
97%
H12
100%
98%
Well
Query
Cover
%
Identity
A02
100%
97%
A03
100%
100%
A04
100%
98%
A05
100%
98%
A06
100%
98%
A07
100%
98%
A08
100%
98%
A09
100%
98%
A10
100%
98%
A11
100%
99%
A12
100%
97%
B02
100%
97%
B03
100%
98%
B04
100%
98%
B05
100%
97%
B06
100%
98%
B07
100%
98%
B08
95%
99%
B09
100%
98%
B10
100%
98%
B11
100%
98%
B12
100%
98%
C01
100%
97%
C02
100%
98%
C03
100%
98%
C04
100%
98%
C05
100%
97%
C06
100%
98%
C07
100%
98%
C08
100%
97%
C09
98%
99%
C10
100%
97%
C11
100%
97%
NR_077068.1
KF468215.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces hansenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
BLAST Match
Phylum
Family
Fungal Profile of SHC COTO11
Accesion #
NR_077068.1
KC009830.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB220032.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
GU017487.1
NR_077068.1
NR_077068.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
KC492933.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Penicillium verrucosum
Ascomycota
Aspergillaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Candida psychrophila
Ascomycota
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Phoma sp.
Ascomycota
Didymellaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Heterobasidion irregulare
Basidiomycota
Bondarzewiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
97
C12
100%
97%
D01
100%
98%
D02
100%
98%
D03
100%
97%
D04
100%
98%
D05
100%
98%
D06
100%
98%
D07
100%
98%
D08
100%
98%
D09
100%
98%
D10
100%
97%
D11
100%
98%
D12
100%
98%
E01
100%
98%
E02
100%
98%
E03
100%
98%
E04
100%
98%
E05
92%
98%
E06
100%
98%
E07
100%
98%
E08
100%
97%
E09
100%
98%
E10
100%
98%
E11
100%
98%
E12
100%
98%
F01
x
F02
100%
99%
F03
100%
98%
F04
100%
98%
F05
100%
97%
F06
100%
98%
F07
100%
98%
F08
100%
98%
F09
10000%
98%
F10
100%
98%
F11
100%
98%
F12
100%
98%
G01
100%
98%
G02
100%
98%
G03
100%
98%
G04
100%
98%
G05
100%
97%
G06
100%
98%
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
HE798150.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
HQ607983.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AB220032.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Acremonium alternatum
Ascomycota
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Candida psychrophila
Ascomycota
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
98
G07
100%
98%
G08
100%
98%
G09
100%
97%
G10
100%
98%
G11
100%
98%
G12
100%
97%
H01
100%
98%
H02
97%
99%
H03
100%
98%
H04
100%
97%
H05
100%
98%
H06
100%
99%
H07
100%
97%
H08
100%
97%
H09
100%
98%
H10
100%
97%
H11
100%
98%
H12
100%
98%
Well
Query
Cover
%
Identity
A02
100%
97%
A03
100%
98%
A04
100%
98%
A05
100%
97%
A06
100%
97%
A07
100%
98%
A08
100%
97%
A09
100%
98%
A10
100%
97%
A11
100%
98%
A12
100%
98%
B02
100%
97%
B03
100%
97%
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
KC311489.1
NR_077068.1
NR_077068.1
NR_077068.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Pseudotaeniolina globosa
Ascomycota
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Fungal Profile of SHC COTO12
B04
99%
98%
B05
100%
100%
B06
100%
98%
B07
100%
97%
B08
100%
98%
B09
100%
98%
B10
100%
98%
B11
100%
98%
Accesion #
BLAST Match
Phylum
Family
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
KF468215.1
HQ832794.1
KF468215.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces hansenii
Ascomycota
Debaryomycetaceae
Cladosporium cladosporioides
Ascomycota
Cladosporiaceae
Debaryomyces hansenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
99
B12
100%
97%
C01
100%
99%
C02
100%
98%
C03
100%
99%
C04
100%
98%
C05
100%
98%
C06
100%
98%
C07
100%
98%
C08
100%
98%
C09
100%
98%
C10
100%
98%
C11
100%
100%
C12
100%
98%
D01
100%
97%
D02
100%
97%
D03
100%
97%
D04
100%
98%
D05
100%
98%
D06
100%
98%
D07
100%
98%
D08
98%
99%
D09
100%
97%
D10
100%
97%
D11
100%
97%
D12
100%
98%
E01
100%
98%
E02
100%
98%
E03
100%
99%
E04
100%
98%
E05
100%
97%
E06
x
E07
100%
97%
E08
100%
98%
E09
100%
98%
E10
100%
98%
E11
100%
97%
E12
100%
98%
F01
100%
98%
F02
100%
98%
F03
100%
98%
F04
100%
98%
F05
100%
98%
F06
100%
97%
JN942672.1
NR_077068.1
JN942672.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
AY568066.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Ascomycete sp.
Ascomycota
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
100
F07
100%
98%
F08
100%
98%
F09
100%
97%
F10
100%
98%
F11
100%
97%
F12
100%
98%
G01
100%
98%
G02
100%
98%
G03
100%
98%
G04
100%
98%
G05
100%
97%
G06
100%
97%
G07
100%
98%
G08
100%
98%
G09
100%
97%
G10
100%
98%
G11
100%
98%
G12
100%
97%
H01
100%
97%
H02
100%
98%
H03
100%
98%
H04
100%
97%
H05
100%
98%
H06
100%
98%
H07
100%
98%
H08
100%
98%
H09
100%
99%
H10
100%
98%
H11
100%
97%
H12
100%
97%
JN942672.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
NR_077068.1
KF468215.1
NR_077068.1
NR_077068.1
NR_077068.1
Debaryomyces fabryi
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces hansenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
Debaryomyces udenii
Ascomycota
Debaryomycetaceae
101