EXPLORING LIFE EXERCISE 2: THE PROTISTS Exercise 2: Protista Workbook Contents Corresponding Section on CD Vocabulary Key Concepts Introduction 1. Introduction General Features of Protists 2. General Features of Protists Animal-like Protists 3. Animal-like Protists 3A. Introduction 3B. Rhizopoda - the Amoebas 3C. Zoomastigophora - the Flagellates 3D. Apicomplexa 3E. Ciliophora Rhizopoda Zoomastigophora Apicomplexa Ciliophora Review Chart Fungus-like Protists Oomycota Chytridiomycota Acrasiomycota Myxomycota Going Further Review Chart Plant-like Protists Chrysophyta Pyrrophyta Rhodophyta Phaeophyta Chlorophyta Review Chart 4. Fungus-like Protists 4A. Introduction 4B. Oomycota 4C. Chytridiomycota 4D. Acrasiomycota - Cellular Slime Molds 4E. Myxomycota - Acellular Slime Molds 5. Plant-like Protists 5A. Introduction 5B. Chrysophyta - Golden-Brown Algae 5C. Pyrrophyta - Dinoflagellates 5D. Rhodophyta - Red Algae 5E. Phaeophyta - Brown Algae 5F. Chlorophyta - Green Algae Summary & Review Vocabulary Anisogamous - Refers to a breeding system characterized by motile gametes of unequal size Antheridium - Structure that produces male gametes Auxospore - Zygote of diatoms Autotroph - An organism that is self-nourishing, i.e., it does not derive nutrients or energy from the digestion of other organisms Axoneme - The central portion of a flagellum or cilium Biflagellate - Having two flagella 2-1 Blade - A broad, relatively flat portion of a brown alga's body that resembles a leaf Carpogonium - The female gametangium of red algae Carpospore - Diploid spore produced by the zygote of red algae Carposporophyte - The adult in the red algae life cycle that produces carpospores Cellulose - A polymer of glucose that forms the major constituents of cell walls in plants and some algae Centric - Circular in shape, i.e., showing radial symmetry Chitin - A nitrogen-containing polysaccharide that forms the exoskeleton of insects and the cell walls of fungi and some protists Chrysolaminarin - The storage compound of the Chrysophyta Cilium - Short flagellum-like structure used in locomotion. Cilia are often arranged in rows. Coenocytic - Having many nuclei in a common cytoplasm Contractile vacuole - A vacuole found in many protists that pumps water out of the protist's body Flagellum - A relatively long whip-like structure used in propulsion. Flagella are composed of microtubules which, in cross-section, are arranged in a characteristic 9+2 structure Frustule - Half of a diatom's shell, i.e., a valve Fucoxanthin - A carotenoid pigment that gives chrysophytes and brown algae their characteristic color Gametangia - Structures that produce gametes Gametophyte - Haploid adult in a sporic life cycle that produces gametes via mitosis Heteromorphic - Refers to species with differently shaped sporophytes and gametophytes Heterothallic - Refers to species that produce male and female gametangia on different individuals Heterotrophic - Organisms that digest other organisms to obtain energy and/or nutrients Holdfast - Basal portion of a brown alga's body that is modified for holding the organism in place Homothallic - Refers to species that produce male and female gametangia on the same individual Hyphae - A filament that comprises a portion of the body of a fungus or fungus-like protist Isomorphic - Refers to a species in which the sporophyte and gametophyte are similarly shaped Laminarin - A polymer of glucose used as the principal storage compound of the brown algae Meiosis - A process characterized by a series of two divisions in which the ploidy number is reduced to half its former value Meristoderm - The tissue layer of a brown alga that is largely responsible for cell division, and thus growth Microcyst - The encysted stage of the ameboid form of a slime mold. Microcysts are derived from only one cell. Mitosis - Normal cell division in which the chromosomes are duplicated and each daughter cell receives a full complement of chromosomes Mycelium - The body of a fungus or fungus-like protist, i.e., a hyphal mass Oogonium - The reproductive structures of some algae. Oogonia produce one or more eggs and are usually unicellular. Pellicle - Protein-rich strips found just inside the membrane and give the bodies of flagellates their shape Pennate - Shape of a bilaterally symmetrical diatom Phycobilins - Water-soluble pigments that occur in red algae and are involved in photosynthesis as accessory pigments Plasmodium - A membrane-bound mass of cytoplasm that contains many nuclei Ploidy - Refers to the number of complements of chromosomes in a nucleus (e.g., haploids have one complement, diploids have two complements and polyploids have many) Plurilocular sporangia - Found in brown algae and produce diploid zoospores Pseudoplasmodium - A mass of unicellular ameboid cells. Pseudoplasmodia occur in the cellular slime molds Pseudopods - A cytoplasmic projection that functions in locomotion. Pseudopods are produced by some protists and some animal cells. Sclerotium - An encysted form of a slime mold. Sclerotia are often produced as a result of desiccation. Spermatium - non-motile male gametes Sporophyte - Diploid adult in the life cycle that produces spore via meiosis Stipe - Staff supporting a blade in brown algae 2-2 Tetrasporophyte - The diploid adult in some red algae's life cycle that produces four spores via meiosis. The spores are produced inside the spore mother cell. Theca - Plates made of cellulose that comprise a portion of the exterior body of a dinoflagellate Thylakoid disc - A membrane-bound sack that normally occurs in stacks. Each sack contains pigments involved in photosynthesis. Trichogyne - A projection from the female gametangium of some red algae and fungi that captures or conveys male gametes into the female gametangia Unilocular sporangia - Produce haploid zoospores via meiosis and occur in brown algae Zoospore - Flagellated spore 2-3 Key Concepts Introduction 1. Protists form an evolutionary link from to . 2. The groups of protists are arranged together based on their and Match each group to its mode of nutrition and life cycle by drawing lines to connect them: Group: Animal-like Fungus-like Plant-like extracellular digestion ingestion photosynthesis alternation of generations gametic Mode of Nutrition: Life Cycle: . zygotic 3. Complete the chart below with the choices provided: Finish phrase in this line: with these choices: for these life cycles: gam etic zygotic alternation of generations: adult #1 adult #2 Adult is divides by diploid/ m eiosis/ haploid m itosis Gam etes fuse; form Zygote to form zygote which is divides by gam etes/ m eiosis/ spores diploid/ haploid m itosis Spores and divide by to form are to form adult adult/ diploid/ m eiosis/ spore haploid m itosis 4. The majority of protists divide by meiosis / mitosis. Where in the life cycle meiosis / mitosis occurs varies among the groups. General Features of Protists 5. Put a check under the correct group heading for each feature: Has Prokaryote Eukaryote Has a. nucleus Prokaryote f. organelles (mitochondria, endoplasmic reticulum, etc.) b. linear chromosomes g. flagella with 9 + 2 structure c. nuclear membrane h. flagella without 9 + 2 d. circular chromosome i. basal body to anchor flagella e. microtubules/ cytoskeleton j. flagella anchored to cell wall 6. True or False: There is one feature that unites all protists. Animal-like Protists 7. All animal-like protists are unicellular / multicellular and some ingest their prey / photosynthesize. Rhizopoda 8. All Rhizopoda have / lack flagella. The name means . Structure 9. Amoebae have / lack a cytoskeleton, have / lack a nucleus, and have / lack a definite shape. 10. Amoebae move by forming . 2-4 Eukaryote The Protists Rhizopoda (continued) Movement 11. Number these steps (from 1 to 8) in the correct order. You may need to pause or replay the movie to determine the steps: microfilaments are carried forward by cytoplasmic actin fibers in trailing portion interact with myosin movement actin units reassemble microfilaments in trailing portion disassemble cytoplasm moves from area of greatest pressure outer layer of cytoplasm (cortex) contracts (center) to less pressure (pseudopod) tip of pseudopod becomes anchored cytoplasm is squeezed into central portion of cell Nutrition 12. Draw and label a series of pictures to describe how amoebae engulf their prey. Be sure to use the terms pseudopod, food vacuole, and lysosome. This process is called endocytosis / phagocytosis / pinocytosis. Life Cycle 13. Asexual reproduction by meiosis / mitosis (binary fission) / none. Sexual reproduction by meiosis / mitosis / none. Zoomastigophora 14. All of the organisms in this group have one or more cilia / flagella / neither. Structure 15. Write the number for the following structures on this Euglena and then match the structure to its function. Number Structure Function chloroplast contain DNA contractile vacuole movement flagellum photosynthesis nucleus preserve water balance stigma sense light 16. True or False: All Zoomastigophora have the same structures as Euglena. Movement 17. Cilia / flagella tend to be short and numerous. Cilia / flagella tend to be longer and fewer in number. 18. Sketch a cross-section of a flagellum, showing the 9 + 2 structure of microtubules: 19. The microtubules are attached at the base by an auxospore / axoneme. 20. Microtubules are hollow / solid and made of the protein dynein / tubulin. 21. The protein dynein / tubulin forms the side arms of the microtubules. 22. Dynein / tubulin catalyzes the hydrolysis of ADP / ATP , and the energy that is released bends the microtubules. Nutrition (pause and use the “Key Words” to define the following two bolded terms, then answer Question #23) 23. Some flagellates are autotrophic / heterotrophic and use photosynthesis, though many are also autotrophic / heterotrophic and absorb the needed nutrients or capture their prey through phagocytosis. Life Cycle 24. Asexual reproduction by meiosis / mitosis (binary fission) / none. Sexual reproduction by meiosis / mitosis / none. 2-5 Key Concepts Apicomplexa 25. All sporozoans are photosynthetic / parasitic / predatory. They acquire nutrients through osmosis / phagocytosis. Structure 26. All sporozoans produce a near the of the cell. Other than this, they are the same / vary. Movement 27. Sporozoans move by means of cilia / flagella / wriggling. Life Cycle 28. True or False: Many Apicomplexa have both an asexual and sexual stage and may require one or more hosts in their life cycle. 29. Number these steps (from 1 to 13) in the correct order. You may need to pause or replay the movie to determine the steps: fertilization and formation of a zygote occurs mosquito bites uninfected person and transfers sporozoites gametocytes change into gametes in mosquito’s gut sporozoites develop inside the oocyst meiosis occurs in the oocyst sporozoites invade liver and lymph system merozoites form new gametocytes sporozoites migrate into mosquito’s salivary glands merozoites infect red blood cells sporozoites reproduce asexually and produce merozoites merozoites reproduce and burst red blood cells zygote produces an oocyst mosquito bites infected person and ingests plasmodium gametocytes Ciliophora 30. The ciliates are among the most complex / simple protists. They have a definite / indefinite size and shape. Structure 31. Draw lines from the names to the structures on this Paramecium. anal pore cilia gullet contractile vacuole macronucleus oral groove micronuclei food vacuole pellicle 32. The anal pore / contractile vacuole / gullet regulates water balance within the cell by expelling excess water. 33. The macronucleus / micronuclei is/are involved in transcription and translation. The macronucleus / micronuclei is/are involved in recombination. Movement 34. Ciliates move by the rhythmic beating of their cilia / flagella which function very much like cilia / flagella. 35. Some ciliates have fused cilia called cirri / myonemes / neurofibrils that they can walk on. 36. True or False: All ciliates are capable of movement in all stages of their lives. Nutrition 37. True or False: Ciliates have a variety of means for capturing their prey; all are, therefore, heterotrophs. 2-6 The Protists Life Cycle 38. Asexual reproduction occurs by means of meiosis / mitosis (simple fission). 39. True or False: Recombination is linked to sexual reproduction through a process called conjugation. 40. In your own words, what is the purpose of recombination? How many cells does it involve at the beginning? at the end? Review Chart For this section, mark an “X” (unless directed otherwise) in the box(es) that correspond to characteristics found within each group or possessed by an example organism from that group. Then use this chart to answer the questions below and in the overall review. Characteristics (generally) Rhizopoda Zoomastigophora Apicomplexa Structures has no definite shape has one nucleus per cell can have more than one nucleus per cell can have chloroplasts has special structures to infect host has cell walls has contractile vacuole can be unicellular can be filamentous can be colonial can be multicellular Life Cycle (fill in for each) Choices: alternation of generations (abbreviate AOG), gametic (GAM), zygotic (ZYG) Reproduction asexual binary fission/mitosis conjugation formation of zoospore sexual lacks requires special structures requires host has a dormant or resting stage Movement pseudopods flagella cilia wriggle none as adult Nutrition can be autotrophic/photosynthetic has a carbohydrate storage product can be heterotrophic; is/uses decomposer extracellular digestion osmosis parasitic phagocytosis 2-7 Ciliophora Key Concepts What characteristic(s) seem to unite the animal-like protists? Which characteristic(s) seem to be unique to each group? Rhizopoda: Zoomastigophora: Apicomplexa: Ciliophora: Fungus-like Protists 41. All of these organisms have like the fungi; they are either autotrophs / decomposers or parasites / predators. 42. Most groups contain both species that are multicellular / unicellular and those that have a colonial / filamentous body. Each filament in the body is know as a hypha / mycelium and the mass that is the whole organism is the a hypha / mycelium. Oomycota 43. This group includes filamentous / unicellular / both forms. The filamentous / unicellular forms are coenocytic, which means they have many chromosomes / hyphae / nuclei in a common cytoplasm. Structure 44. Match the group with its characteristics. What do scientists believe, based on this information? Group Cell Wall Composed of Fungi cellulose Oomycota chitin Has Flagellated Cell(s) at Some Point in Life Cycle Chromosomes Diploid highly condensed adult no not highly condensed one cell in life cycle yes Life Cycle 45. Label the asexual reproduction cycle of an example oomycete. Be sure to include the terms active, adult, diploid, encysted, flagella, germinate, secondary, septum, zoosporangium, zoospore. You may use some terms more than once. 2-8 The Protists Oomycota (continued) 46. Label the sexual reproduction cycle of an example oomycete. Be sure to label the: adult, antheridium, dormant, eggs, female nuclei, fertilization tubes, fusion, germinate, haploid, male nuclei, meiosis, oogonium, oospore, zoosporangium, zygote, n, 2n Chytridiomycota 47. Circle all of the characteristics that apply to this group. Headings are italic and to the left; choices are then to the right. Where they live: Organism “body”: Cell wall of: Spores & gametes: Nutritional lifestyle: aquatic and terrestrial filamentous cellulose motile spores decomposers mostly aquatic unicellular chitin non-motile spores parasites mostly terrestrial coenocytic uninuclear flagellated gametes photosynthetic unflagellated gametes predators 48. Mark (check, star or underline) all of the above characteristics that this group shares with the fungi. What other characteristic points to a strong relationship between these two groups? Structure 49. Isomorphic = diploid & haploid adult stages look different / the same; heteromorphic = adult stages look different / the same. Life Cycle 50. What type of life cycle does this chytridiomycete display? gametic / zygotic / alternation of generations Label the life cycle of a typical chytridiomycete. Use the terms asexual, diploid (2n), female, fertilization, flagella, fusion, gametangium, gametes, gametophyte, germinate, haploid (n), hyphae, male, mature, meiosis, mitosis, mycelium, sexual, sporangium, sporophyte, young, zoospores, zygote. What is the purpose of the hormone sirenin in this life cycle? 2-9 Key Concepts Acrasiomycota 51. The habitat of the cellular slime molds is generally aquatic / terrestrial / both. The myxamoebas are decomposers / through parasites / photosynthetic / predators, feeding on . Structure 52. Write the appropriate characteristic under each group heading: Characteristic choices: Acrasiomycota Fungi Protists/Amoeba unicellular or multicellular cell wall of cellulose or chitin or no cell wall centrioles or no centrioles nuclear envelope breaks down during mitosis or does not has motile cells or does not has contractile vacuoles or does not Life Cycle Asexual Reproduction 53. As long as the myxamoebae can find bacteria, they reproduce by means of meiosis / mitosis. 54. Number these steps (from 1 to 11) in the correct order. You may need to pause or replay the movie to determine the steps: anterior cells enter the starvation phase & produce a stalk the pseudoplasmodium aggregate moves away basal and stalk cells die the pseudoplasmodium aggregate stops cell with the highest cyclic AMP frequency becomes the center spores are dispersed individual myxamoebae begin to aggregate spores land on warm, moist soil and produced new myxamoebae myxamoebae secrete cyclic AMP the supply of bacteria becomes scarce posterior cells enter the starvation & form dormant spores You may need to rewind the previous movie section to answer these questions. 55. The movement of the pseudoplasmodium is by means of contraction of the protein actin / dynein / myosin. 56. Draw the probable path of a pseudoplasmodium on this graph. Then explain what directs the pseudoplasmodium to this location. Cool Warm Dark Light Sexual Reproduction 57. The diploid / haploid myxamoebae fuse to form a diploid / haploid cell, which then does what? 58. After a thin membrane forms around each cell in the aggregate, the cells are now termed 59. Then, a thick wall of cellulose / chitin is laid down around the entire aggregate, while inside each cell undergoes to form haploid myxamoebae, which then become free living. 2-10 The Protists Myxomycota 60. True or False: This group is not related to any other known group. They do not have / have cell walls. They are by means of autotrophs / heterotrophs because they feed on they can also though . Structure 61. Plasmodial slime molds are relatively large / small and have many / one nuclei. 62. Briefly describe the movement of plasmodial slime molds: Life Cycle 63. Limited amounts of which two factors cause plasmodial slime molds to move? and 64. When the plasmodium stops, a gametangium / sporangium forms, producing diploid / haploid meiosis / mitosis. How many of the resulting cells will mature? . gametes / spores through Is it active or dormant? 65. Draw and label a picture of the two forms that may result after germination and explain how they are interconvertible. Be sure to include the terms amoeboid, haplophase, flagellated, gamete, plasmogamy, zygote. 66. When it is dry, these organisms can form a resilient resting stage called a microcyst / sclerotium. If they run out of food or conditions become harsh, they can also form a microcyst / sclerotium that is viable for more than a year. Going Further The following terms also relate to the fungus-like protists. Use your key words to define the terms, and then answer these questions: 67. Homothallic = Male & female sex organs on different / same individual; heterothallic = organs on different / same individual. 68. Is the example oomycete (Saprolegnia) heterothallic or homothallic ? Explain: 69. Is the example chytridiomycete (Allomyces) homothallic or heterothallic? Explain: 70. Anisogamous means that the gametes are motile / non-motile and are different / the same size. 2-11 Key Concepts Review Chart For this section, mark an “X” (unless directed otherwise) in the box(es) that correspond to characteristics found within each group or possessed by an example organism from that group. Then use this chart to answer the questions below and in the overall review. Characteristics Oomycota Chytridiomycota Acrasiomycota Structures no definite shape one nucleus per cell more than one nucleus per cell has chloroplasts/chlorophyll(s) has special structures to infect host has cell walls of cellulose of chitin of other material(s) has contractile vacuole can be unicellular can be filamentous can be colonial can be multicellular and branched can be multicellular, with differentiation Life Cycle (fill in for each) Choices: alternation of generations (abbreviate AOG), gametic (GAM), zygotic (ZYG) Reproduction asexual binary fission/mitosis formation of zoospore aggregation sexual lacks requires special structures forms macrocysts requires host has a dormant or resting stage Cell Division (any? Yes or No) Movement pseudopods flagella cilia wriggle none as adult Nutrition autotrophic/photosynthetic has a carbohydrate storage product heterotrophic decomposer extracellular digestion parasite phagocytosis What characteristic(s) seem to unite the fungus-like protists? Which characteristic(s) seem to be unique to each group? Oomycota: Chytridiomycota: Acrasiomycota: Myxomycota: 2-12 Myxomycota The Protists Plant-like Protists 71. The plant-like protists are mainly autotrophs / heterotrophs, differing from one another in the type of chloroplast / chlorophyll and their: a. accessory photosynthetic pigments b. cell wall constituents c. life cycle d. storage products e. all of the above f. none of the above Chrysophyta 72. Most of the golden-brown algae produce chlorophyll(s) A / B / C / D. Some are heterothallic / heterotrophic. They produce the accessory pigment(s) fucoxanthin / phycobilisomes / xanthophyll. Their storage product(s) is/are chrysolaminarin / laminarin / starch, which is/are stored inside / outside the chloroplast. 73. Some species have one / two / no cell wall(s). The cell walls may have calcium carbonate / cellulose / chitin / silica. 74. This group is closely related to brown / green / red algae. Why? 75. Label the following diatoms. Use the terms centric, frustules, pennate, valves. Label the diatom that floats better and explain why. 76. Describe the ecological and economic importance of the diatoms: Life Cycle 77. Number these structures (1–10) in the order they appear in the life cycle. Then match each to the correct description: Number Structure Description Number Structure Description a. daughter cells form new valve f. functional egg is fertilized b. cells at one third maximum; begin sexual reproduction g. four sperm formed via meiosis and released c. mother cell/frustule h. four eggs form via meiosis; three daughter nuclei disintegrate d. daughter cells separate; one is smaller i. zygote expands; called an auxospore e. daughter cells form, one with each valve j. zygote formed 78. What are the letters of the steps that represent asexual reproduction? 2-13 sexual reproduction? Key Concepts Pyrrophyta 79. The dinoflagellates occur in freshwater only / marine only / both aquatic environments. 80. Label the diagram with the following terms: theca, plasma membrane, flagella, groove, equatorial belt, longitudinal meridian. At what angle, approximately, are the two grooves? 81. What is the function of the pusule? 82. Dinoflagellates have chloroplasts that are similar to cyanobacteria / green algae / red algae. How might they have obtained these chloroplasts? 83. They produce the accessory pigment(s) fucoxanthin / phycobilisomes / xanthophyll. Their storage product(s) is/are chrysolaminarin / laminarin / starch. True or False: All dinoflagellates are autotrophic. 84. Describe the ecological and economic importance of the dinoflagellates: Life Cycle 85. Dinoflagellates are usually diploid / haploid. When reproducing via mitosis, daughter cells each receive 86. Dinoflagellate chromosomes are circular / rod-like / thread-like, highly condensed / not condensed, and attached / not attached to the nuclear membrane, which disintegrates and reforms / remains intact during cell division. 87. True or False: Non-motile species produce flagellated zoospores, the only part of the life cycle that resembles the typical dinoflagellate. 88. Low levels of what element operate as a limiting factor and induce sexual reproduction? 89. Gametes are produced via meiosis / mitosis. Why? 90. Isogamy = gametes different size / same size; Anisogamy = gametes different size / same size. 91. The fusion of two compatible gametes results in the formation of a diploid / haploid , which forms a resting cyst. When the cyst breaks dormancy, it undergoes meiosis / mitosis. Why is this significant? Rhodophyta 92. Red algae are multicellular / unicellular and important in freshwater / marine / terrestrial environments. 93. True or False: The coraline algae produce sodium carbonate in their cell walls. 94. Circle all of the characteristics that apply to this group. Headings are italic and to the left; choices are then to the right. Number of nuclei per cell: one more than one Flagellated cells in life cycle: Centrioles: have lack have ones with 9 + 2 pattern lack; have a structure without 9 + 2 pattern Cell division: binary fission complete furrowing incomplete furrowing w/ pit connections Thylakoid disks: short separated stacked xanthophyll inside thylakoid disks long Accessory pigment(s): fucoxanthin phycobilisomes Chlorophyll(s): C A B D Storage compound(s): chrysolaminarin laminarin starch Cell walls: chitin mannose cellulose 2-14 silica xylose plate formation outside thylakoid disks The Protists Rhodophyta (continued) Life Cycle 95. Number these steps (from 1 to 14) in the correct order: carpospores germinate into diploid tetrasporophytes spermatium nucleus migrates down into the carpogonium to fertilize the egg carpospores released into the current carposporophyte produces diploid carpospores via mitosis tetraspores germinate into haploid male or female gametophytes female and male gametophytes produce eggs and spermatia, respectively, via mitosis tetraspores released into the current tetrasporophyte produces tetraspores via meiosis female gametophyte develops carpogonial branches; male develops spermatangial branches unflagellated spermatia released into the current filamentous carposporophyte remains associated with the female gametophyte zygote divides via mitosis to form a diploid adult carposporophyte spermatium attaches itself to the trichogyne zygote formed Phaeophyta 96. Brown algae can live in freshwater only / marine only / both aquatic environments and range from colonial / unicellular forms to colonial / multicellular forms with cell differentiation. 97. Label the structures of this brown algae body using the terms blade, holdfast and stipe. 98. Brown algae produce chlorophyll(s) A / B / C / D and accessory pigment(s) fucoxanthin / phycobilisomes / xanthophyll. Life Cycle 99. The two adult stages of brown algae are the diploid / haploid gametophyte and the diploid / haploid sporophyte. Isomorphic = two adult stages look alike / different; this is the condition found in advanced / primitive brown algae. Heteromorphic = two adult stages look alike / different; this is the condition found in advanced / primitive brown algae. 100. Plurilocular sporangia are involved in asexual / sexual reproduction. They produce diploid / haploid unflagellated flagellated / gametes / zoospores that develop directly into gametophytes / sporophytes. Sporophytes also have uni- locular sporangia that produce diploid / haploid gametes / zoospores that germinate into gametophytes / sporophytes. Gametophytes have plurilocular / unilocular gametangia, the antheridia or female / male parts that produce flagellated / unflagellated sperm and the oogonium or female / male part. Fertilization occurs at the base / tip of the oogonium. The zygote remains and grows into a new gametophtye / sporophyte. 2-15 Key Concepts Chlorophyta 101. Describe the living conditions of some of the many green algae: 102. Write the appropriate characteristic under each group heading: Characteristic Choices (choose all appropriate; use abbreviations) Chlorophyta chlorophyll(s) A, B, C, D cells walls cellulose (ce), chitin (ch), hemicellulose (h), pectin (p), silica (s) storage product(s) chrysolaminarin (c), laminarin (l), starch (st) life cycle alternation of generations (AOG), gametic (G), zygotic (Z) cell division binary fission (BF), furrowing (F), plate formation (PF) forms unicellular (u), colonial (col), filamentous (fil), multicellular (m) 2-16 Plants The Protists Review Chart For this section, mark an “X” (unless directed otherwise) in the box(es) that correspond to characteristics found within each group or possessed by an example organism from that group. Then use this chart to answer the questions following and in the overall review. Characteristics Chrysophyta Pyrrophyta Rhodophyta Structures one nucleus per cell more than one nucleus per cell has cell walls/plates of cellulose of chitin containing silica with calcium carbonate of other material(s) none can be unicellular can be filamentous or colonial can be multicellular Life Cycle (fill in for each) Choices: alternation of generations (abbreviate AOG), gametic (GAM), zygotic (ZYG) Reproduction asexual binary fission/mitosis formation of zoospore sexual lacks requires special structures has a dormant or resting stage Cell Division (if any) plate formation furrowing Movement flagella cilia wriggle none as adult Nutrition autotrophic/photosynthetic can be heterotrophic; is/uses phagocytosis has chlorophyll(s) a b c d has accessory pigment(s) fucoxanthin phycobilisomes xanthophyll uses as storage product(s) chrysolaminarin laminarin starch What characteristic(s) seem to unite the plant-like protists? Which characteristic(s) seem to be unique to each group? Chrysophyta: Pyrrophyta: Rhodophyta: Phaeophyta: Chlorophyta: 2-17 Phaeophyta Chlorophyta Summary & Review Look back over the charts that you have completed for each of the groups of protists – animal-like, fungus-like and plant-like – and answer the following questions using those charts. 1. What are some of the differences between protists and monerans? What are some of the similarities (if any)? 2. Is there a single defining feature of the Kingdom Protista? Explain your answer: 3. Which groups are the most animal-like? What does “animal-like” mean? Are there any characteristics of the “animal-like” protists that they share as a group and that set them apart from other protists? Explain: 4. Which group(s) might the animals have evolved from? What evidence supports this? 5. Which groups are the most fungus-like? What does “fungus-like” mean? Are there any characteristics of the “fungus-like” protists that they share as a group and that set them apart from other protists? Explain: 6. Which group(s) might the fungi have evolved from? What evidence supports this? 7. Which groups are the most plant-like? What does “plant-like” mean? Are there any characteristics of the “plant-like” protists that they share as a group and that set them apart from other protists? Explain: 8. Which group(s) might the plants have evolved from? What evidence supports this? 9. Which groups have sexual reproduction? What distinguishes their life cycles? Which groups do not have sexual reproduction? Explain why you think that they may not have sexual reproduction: 10. In your own words, define “heterotrophic”: What are some methods by which heterotrophic organisms obtain food? In your own words, define “autotrophic”: Which organisms are both heterotrophic and autotrophic? What might be the advantages/disadvantages of being both? 11. Which groups are heterotrophic and mobile as adults? Which groups are heterotrophic and not mobile as adults? Is heterotrophy always associated with movement? 2-18 The Protists 12. What is the ecological importance of some of the protists? List at least 5 and explain: 13. What is the economic/human health importance of some protists? List as least 5 and explain: 14. In your own words, what is endosymbiosis? What support is there to support the endosymbiotic theory? Be sure to list specific organisms. 15. Write the letter of the correct group next to each diagram. If the structure in the diagram has a special name, write it next to the structure: A. Acrasiomycota B. Apicomplexa C. Chlorophyta D. Chrysophyta E. Chytridiomycota F. Ciliophora G. Myxomycota H. Oomycota I. Phaeophyta J. Pyrrophyta K. Rhizopoda L. Rhodophyta M. Zoomastigophora 2-19
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