Aspergillus Media
Minimal Medium
50X MM Salts
Component
NaNO3
KCl
MgSO4:7H2O
Amt./Liter
300 gm
26 gm
24.65 gm
Dissolve each in the order given and store at room temperature over a few drops of chloroform.
1M Phosphate Buffer pH~6.8
Component
KH2PO4
K2HPO4
Making Minimal Medium (MM)
Component
50X Salts
Dextrose
1M KPO4 pH 6.8*
Trace Elements
Agar
Amt./Liter
68 gm
87.1 gm
Amt./Liter
20 ml
10 gm for plates or 3.6 gm for liquid.
12 ml * Add after autoclaving for liquid media to prevent precipitate
forming.
1 ml
12.5 gm For solid media.
Complete Medium (CM)
Add the following to MM.
Component
Yeast Extract
Peptone
Tryptone
Vitamin Mix
CM Supplement
Amt./Liter
1 gm
2 gm
1 gm
2 ml
10 ml
Malt Extract Medium (MAG)
Component
Malt Extract
Peptone
Dextrose
Trace Elements
Vitamin Mix
Agar
Amt./Liter
20 gm
2 gm
10 gm
1 ml
2 ml
20 gm For solid media.
Yeast Extract Medium (YAG)
Component
Yeast Extract
Dextrose
Trace Elements
Vitamin Mix
1M MgSO4 or Cl2
Agar
Amt./Liter
5 gm
10 gm
1 ml
2 ml
10 ml
15 gm For solid media.
TRACE ELEMENTS SOLUTIONS
Cove's Trace Elements Solution
Component
Na2B4O7:10H2O
CuSO4:5H2O
Ferric Citrate or chloride
MnSO4:H2O
NaMoO4:2H2O
ZnSO4:7H2O
Amt./Liter
40 mg
400 mg
800 mg
800 mg
800 mg
8 gm
Dissolve each fully in the order shown, before adding the next. This stock is 1000 fold concentrated. Store at room
temperature over a few drops of chloroform. A precipitate will eventually form, just shake the bottle before using.
Clive Roberts Trace Elements Solution
Component
FeSO4.7H2O
ZnSO4.7H2O
CuSO4.5H2O
MnSO4.H2O
Na2B4O7.10H2O
(NH4)6Mo7O24.4H2O
Amt./Liter
1 gm
8.8 gm
400 mg
150 mg
100 mg
50 mg
Dissolve each in the order given. This stock is 1000 fold concentrated. Store at room temperature over a few drops of
chloroform.
Greg’s Modified Trace Elements
Component
0.25 M EDTA pH8.0
FeSO4 7H2O
ZnSO4 7H2O
CuSO4 5H2O
MnSO4 H2O
Na2B4O7 10H2O
NaMoO4 2H2O
CaCl2 2H2O
Amt./liter
100 ml
1 gm
8.8 gm
0.4 gm
0.15 gm
0.1 gm
0.1 gm
0.1 gm
mM
25
3.6
30.1
1.6
0.89
0.26
0.41
0.68
Dissolve each in the indicated order being sure that the first is fully dissolved before adding the next. This is 1000
fold concentrated. Store at room temperature.
VITAMIN MIX
Component
p-Aminobenzoic acid
Niacin
Pyridoxine HCl
Riboflavin
Thiamine HCl
Choline HCl
d-Biotin
Amt./Liter
1 gm
1 gm
1 gm
1 gm
1 gm
1 gm
2 mg
Store at 4oC in the dark after autoclaving.
CM SUPPLEMENT
Component
Adenine HCl
L-Methionine
L-Lysine or Arginine
Riboflavin
Amt./Liter
5 gm
5 gm
36.5 gm
0.5 gm
Store at 4oC in the dark after autoclaving.
From the May laboratory