The importance of oil
for IVF outcome
Most clinics today use oil to cover embryo culture
dishes in order to maintain stable temperature,
osmolality and pH. The quality of such oil plays a
very important role for IVF success. The oil types
commonly used are mineral oil, paraffin oil or a
mixture of both these oil types.
Paraffin oil and mineral oil are chemically slightly
different. The polycarbon lipid tail of mineral oil
contains more unsaturated bonds than the more
saturated paraffin oil. This makes mineral oil more
unstable and prone to attack by free oxygen radicals
and photo-oxidation than stable paraffin oil.
In a study by Tae et al. published in 2006, bovine
embryo development was studied in cultures
overlaid with either washed mineral or sterile
paraffin oil, Fig 1.
t rates improved
Percent
• 100% paraffin oil of
highest quality.
• Extensively tested.
• Confirmed excellent
embryo development.
Embryo development
under different oil types
50
40
30
20
10
0
Morula
Blastocyst
Washed mineral oil
Sterile paraffin oil
Fig 1. Embryo development
under different oil types
Percent
OVOIL
™
Embryo development
under different oil types
50
Washed mineral oil
Sterile paraffin oil
40
30
20
10
0
Morula
Washed mineral oil
Blastocyst
Comparison Mineral oil and paraffin oil
Sterile paraffin oil
Sterile filtered paraffin oil overlay resulted in
significantly higher (p < 0.05) development rate
to morula (44.8% versus 30.6%) and blastocyst
(32.8% versus 21.7%) than washed mineral oil.
REF: Tae, J.C. et al. J. Assist Reprod & Gen. 2006
40
40
Superior embryo
viability with OVOIL™
20
20
Cook Medicult Sage
0
Cook Medicult Sage
Day 3
Day 4
Sigma
Vitrolife
Day 5
Day 5 hatching
Oil is not just oil
Oils from 6 different manufacturers were tested
and embryo develop­ment and viability was
and Fig 2. OVOIL™ showed
Fig evaluated.
3. EmbryoFig 1
viability
80
4
2
Irvine Vitrolife
11.2
14.3
11.7
12.6
19.9
25.3
22.6
32.8
13.6
31.2
44.0
54.7
47.1
67.4
40.2
61.8
78.7
22.4
8.2
11.6
10.2
12.0
35.7
N=99
N=97
N=98
N=96
N=97
N=97
significantly
better
development
(p<0.01)
for
all
embryo
viability
parameters
studied,
number of
Day 3
Day 4
Day 5
Day 5 hatching
cells in the trophectoderm and in the inner cell
mass, and total number of cells in the blastocysts.
D
20 40
60
40
0 20
20
Sigma
0 Trophectoderm
Total
Cook Medicult Sage Sigma
Vitrolife
0
ICM
Irvine Vitrolife
80
60
20
2
0
Cook Medicult
SigmaSage
Irvine Sigma
Vitrolife Vitrolife
Cook Sage
Medicult
11.2
14.3
11.7
12.6
19.9
54
5312.1
57 11.2 42
14.357
61
11.7
19.9
25.3
22.6
32.8
13.6
31.2
44.0
65
6525.3
67 22.6 52
32.868
76
13.6
44.0
54.7
47.1
67.4
40.2
61.8
78.7
11
1254.7
11 47.1 9
67.412
15
40.2
78.7
22.4
8.2
11.6
10.2
12.0
35.7
17%
18%
22.4
16%8.2
11.617%
20%
10.2
35.7
N=99
N=97
N=98
N=96
N=97
N=97
N=99
N=97
N=99
N=98N=97 N=96 N=98
N=97
N=97
N=96
N=97
Day 3
Day 4
Day 5
Day 5 hatching
Trophectoderm
Day 3
Sperm
sensitivity testing
60
1-cell mouse embryo assays
•40Sensitive CF1 mouse strain on raw material
• 1 cell, F1 hybrid mouse on finished product
• Mouse embryo assays with multiple endpoints
20
for grading development
ÅN
rolife G5 br:
• Blastocyst differentiation staining and cell
0
counts
1: Embryo development
achieved
Sigma
Day 5 hatching
Irvine Vitrolife
80
p<0.1
P To learn more
60
about OVOIL™,
please check our
website at
www.vitrolife.com
40
20
0
Cook Medicult Sage
Sigma
Vitrolife
54
53
57
42
57
61
54
53
57
42
61
65
65
67
52
68
76
65
65
67
52
76
11
12 Sweden.15Tel +46-31-721 80 00, Fax +46-31-72111
12
11
9
15
Vitrolife Sweden
AB, 12
Box 9080,11
SE-400 929 Göteborg,
80 90, [email protected],
www.vitrolife.com
Vitrolife
Street, Englewood,
Colorado
80110, USA. Tel +1-303-762-1933,
Fax
+1-303-781-5615,
[email protected]
%ICM Inc.,
17%3601 South
18% Inca16%
18%
17%
20%
%ICM
16.7%
18.5%
15.5%
18.5%
19.6%
N=99
N=97
N=98
N=96
N=97
T
Fig 3. Embryo viability
p<0.1
OVOIL™ is an extensively quality tested product:
Medicult Sage
ulture under 5 different oilCook
overlays.
DayICM
5
%IC
REF: D. Linck, SIRT, Australia. 2008.
Cell number
80
18%
DayTotal
4
REF: D. Linck, SIRT, Australia. 2008.
Fig 3. Embryo viability
Extensive
quality testing
N=97
6
4
12.1
%ICM
8
40
N=99
N=97
N=98
N=96
N=97
© Vitrolife 1.110426.1
Cook Medicult Sage
Fig
p<0.1
Cell number
Cell number
40 60
80
Embryo development, percent
Fig 2. Embryo
Fig 3. Embryo
viabilitydevelopment
60 80
Cell number
Sigma
12.1
p<0.1
Fig 2. Embryo development
Embryo development, percent
Cell number
6
0
rolife
<0.1
Embryo development,
Embryo development,
Embryo development, perce
60
60