INDIANA UNIVERSITY BLOOMINGTON
Flow Cytometry Core Facility
The Flow Cytometry Core Facility (FCCF) is located in Jordan Hall 029 on the IU Bloomington campus. This
facility provides scientists access to state-of-the-art flow cytometry instrumentation for their research.
The facility is equipped with two analyzers for fluorescent detection of cells and particles. Multiple assays
such as cell cycle, apoptosis, fluorescent protein detection, and more can be performed with samples from
bacteria to cultured cells. The facility is also equipped with two sorters, a high-speed cell sorter and a largeparticle sorter. Cell sorters allow specific subsets of populations to be retrieved for downstream culturing or
analysis. Two additional instruments, a cell counter with cell sizing capability and a single-cell PCR
thermocycler, are also available. Sorters are run by a dedicated facility technician. Researchers may be
trained for independent use of the analyzers, cell counter and thermocycler.
Aria IIu
LSRII w/ HTS
Calibur
COPAS
INSTRUMENTATION
BD FACSAria IIu high-speed cell sorter
• Cell and particle detection and sorting from 0.2μm to 50μm diameter
• Five lasers for excitation at 355nm, 405nm, 488nm, 561nm and 640nm
• 17-parameter detection: 3 light scatter (FSC, SSC, FSC-PMT) and 14 fluorescent
• Four nozzle sizes: 70μm, 85μm, 100μm and 130μm
• Collection devices: four-way sorting into 1.5 and 2ml microcentrifuge and 5ml roundbottom tubes, two-way sorting into 15ml conical tubes, one-way sorting onto slides
and into multi-well plates (6, 12, 24, 48, 96, 384)
• Temperature control and aerosol containment units
BD LSRII high-speed analyzer with High Throughput Sampler (HTS)
• Cell and particle detection from 0.2μm to 50μm diameter
• Four lasers for excitation at 405nm, 488nm, 561nm, and 640nm
• 16-parameter detection: 3 light scatter (FSC, SSC, FSC-PMT) and 13 fluorescent
• HTS for analysis of samples in 96- and 384-well format
BD FACSCalibur analyzer
• Cell and particle detection from 1μm to 50μm diameter
• Two lasers for excitation at 488nm and 633nm
• 6-parameter detection: 2 light scatter (FSC, SSC) and 4 fluorescent
COPAS (Complex Object Parametric Analyzer and Sorter) Select
• Large particle detection from 20μm to 150μm (C. elegans, Drosophila embryos, etc.)
• Single tunable laser (488nm/514nm) for detection of Green, Yellow, and Red
fluorescence
• Collection devices: culture dishes or 96-well plates
Z2
Z2 Coulter counter
• Concentration and size measurement of cells and particles from 2μm to 60μm
AmpliSpeed thermocycler
• compatible with AmpliGrid slide technology for running single-cell PCR on cells
retrieved by sorting with the Aria IIu
AmpliSpeed
Christiane w/ COPAS
Contact information:
Christiane Hassel, MS - [email protected]
812-855-7101
http://facs.bio.indiana.edu/