Merck Millipore Western Blotting 1-2-3 Bundling Offer
Terms & Conditions: 1. 10% off for purchase of any two different categories
2. 15% off for purchase of item(s) in category 1+2+3
3. 25% off for Merck SignalBoost™ Immunoreaction Enhancer Kit if it is purchase with the categorial item(s)
Category 1
Immobilon Transfer Membrane Family:
- Filter dimensions: 26.5 cm x 3.75 m roll
- Filter material: Hydrophobic PVDF
Category 2
Blø k Blocking solutions, 500 mL.
- Membrane coverage 100 cm2 ,19 mini blots (8x7 cm)
- Stable for 2 years at 20-25 oC
Immobilon-P Transfer Membrane (Cat. #IPVH00010)
Blø k - CH Noise Cancelling Reagents
- The original PVDF membrane for western, dot, and other (Cat. #WBAVDCH01)
protein blots
- For chemiluminescence detection
- Applications: Western blotting, dot/slot blotting, mass
spectrometry
Immobilon-PSQ Transfer Membrane (Cat. #ISEQ00010) Blø k - FL Noise Cancelling Reagents
- Small pore structure results in superior binding of
(Cat. #WBAVDFL01)
proteins with MW <20 kDa
- For fluorescent detection
- Applications: Western blotting, dot/slot blotting, mass
spectrometry, fluorescence assay
Immobilon-FL Transfer Membrane (Cat. #IPFL00010)
Blø k - PO Noise Cancelling Reagents
- Optimized for fluororescent immunodetection applications (Cat. #WBAVDP001)
- For phosphoprotein detection using chemiluminescence
or fluorescence techniques
- Applications: Western blotting, dot/slot blotting, MS,
phosphorolation assay (*Stable for 1 year)
Immobilon-P Blotting Sandwich Membrane
- Blotting Sandwich Immobilon-P membrane interleaved
with blotting paper
- Dimensions (cm2): 7x8.4 or 8.5x13.5
- Qty/ Pk: 20
Immobilon Blotting Filter Paper
- Applications: Western blotting, dot/slot blotting
- Filter material: Chromotography-Grade Blotting Paper
- Dimensions (cm2): 7x8.4 or 8.5x13.5
- Qty/ Pk: 100
Web store: http://shopping.netsuite.com/onwon
Tel: 2757 7569
Fax: 2757 7211
Promotion Codes:
Offer 1: MMWB123A
Offer 2: MMWB123B
Offer 3: MMWB123C
Category 3
Immobilon Western Detection Reagents
- Premixed, ready to use chemiluminescent HRP
detection reagent, 500 mL
- Membrane coverage 5,000 cm2
- Stable for 1 year
Luminata Classico Western HRP substrate
(Cat. #WBLUC0500)
- Volume: 500 mL
- Applications: Western blotting, dot/slot blotting
- Detection range: ~ 6 pg
Luminata Crescendo Western HRP substrate
(Cat. #WBLUR0500)
- Volume: 500 mL
- Applications: Western blotting, dot/slot blotting
- Detection range: 1-3 pg
Luminata Forte Western HRP substrate
(Cat. #WBLUF0500)
- Volume: 500 mL
- Applications: Western blotting, dot/slot blotting
- Detection range: 400 fg
Immobilon Western Chemiluminescent HRP Substrate
(Cat. #WBKLS0500)
- Components: Luminol (250 mL); Peroxide solution (250
mL)
- Membrane coverage 5,000 cm2
Merck RapidStep™ ECL Reagent (Cat. #345818)
- One step chemiluminescent substrate reagent for
detection of HRP on immunoblots.
- Volume: 100 mL; Membrane coverage: 2,000 cm2
Others
Merck SignalBoost™ Immunoreaction Enhancer
Kit (Cat. #407207)
- Significantly improves sensitivity and discrimination
when assessing protein targets of low
immunoreactivity or low expression levels.
- Replaces conventional antibody diluents. No
additional steps, materials, or increase of assay time
is required.
- Membrane coverage: 1,600 cm2, 20 miniblots
Customer Testimony from CUHK:
Protein sample: Rat brain (40ug)
Materials Used:
Signalboost immunoreaction enhancer kit
Solution 1 for Primary Antibodies: 1 bottle, 200 ml
Solution 2 for Secondary Antibodies: 1 bottle, 200 ml
Blot A:
Serotonin Transporter polyclonal antibody (Millipore)
diluted 1:1000 in 2.5% Milk
Anti-rabbit secondary antibody (Cell Signaling)
diluted 1:1000 in 2.5% Milk
Blot B:
Serotonin Transporter polyclonal antibody (Millipore)
diluted 1:10000 in Signalboost
Anti-rabbit secondary antibody (Cell Signaling)
diluted 1:10000 in Signalboost
kDa
A
B
70 –
SERT
50 –
Web store: http://shopping.netsuite.com/onwon
Tel: 2757 7569
Fax: 2757 7211