Takara’s Fast-Cutting
Restriction Enzymes
Save time with speedy restriction enzymes from Takara Bio. All of the restriction
endonucleases listed below can be used for either fast*, 5 minute digestion reactions
or overnight digestion. The choice is yours!
*1 µl of each Takara restriction enzyme (RE) listed below is sufficient to completely and specifically digest 1
µg plasmid in just 5 minutes, using standard buffer and temperature conditions.
Product Name
Cat. No.
Aat II
1112A
Pack Size
Product Name
Cat. No.
100 U
Hha I
1056A
Pack Size
2000 U
Hinf I
1238A
6000 U
Hinf I (HC)*
1238AH
6000 U
Hpa I
1064A
500 U
Acc I
1001A
100 U
Afa I (Rsa I)
1116A
1000 U
Alu I
1004A
500 U
Apa I
1005A
10000 U
Mbo II
1145A
400 U
Apa I (HC) *
1005AH
10000 U
Nae I
1155A
500 U
BamH I
1010A
10000 U
Nco I
1160A
500 U
BamH I (HC)
1010AH
10000 U
Nco I (HC)*
1160AH
500 U
Ban II (HgiJ II)
1012A
2000 U
Not I
1166A
500 U
BciT130 I (EcoR II, Mva I)
1232A
2000 U
Not I (HC)*
1166AH
1168A
500 U
Bgl I
1020A
1000 U
Nru I
Bgl II
1021A
2000 U
PshA I
1074A
200 U
Bgl II (HC) *
1021AH
2000 U
PshB I (Vsp I)
1109A
1000 U
1000 U
Bln I (Avr II)
1022A
400 U
Pst I
1073A
10000 U
BmeT110 I (Ava I)
1207A
500 U
Pst I (HC)*
1073AH
10000 U
Pvu II
1243A
4000 U
Pvu II (HC)*
1243AH
4000 U
Sac II
1079A
1000 U
Sau3A I (Mbo I)
1082A
200 U
BmgT120 I (Cfr13 I, Asu I)
1231A
1000 U
Bsp1286 I (Sdu I)
1024A
500 U
BspT104 (Asu II, Nsp V)
1225A
2500 U
BssH II (BseP I)
1119A
300 U
BstP I (BstE II, Eco0651)
1025A
2000 U
Sau3A I (Mbo I) (HC)*
1082AH
1084A
1500 U
200 U
BstX I
1027A
1000 U
Sca I
Cla I
1034A
1000 U
Sfi I
1245A
1000 U
Cla I (HC) *
1034AH
1000 U
Sma I
1085A
2000 U
Cpo I (Rsr II) (Csp I)
1035A
400 U
Sma I (HC)*
1085AH
2000 U
Dra I (Aha III)
1037A
4000 U
SnaB I
1245A
200 U
Dra I (Aha III) (HC) *
1037AH
4000 U
Spe I
1086A
300 U
Eae I (Cfr I)
1123A
200 U
Sph I
1246A
600 U
Eco O65 I (BstE II, BstP I)
1135A
1000 U
Sse8387 I
1183A
400 U
EcoR I
1040A
10000 U
Sse8387 I (HC)*
1183AH
400 U
EcoR I (HC) *
1040AH
10000 U
Stu I
1088A
500 U
EcoR V
1042A
3000 U
VpaK11B I (Ava II)
1196A
300 U
EcoR V (HC) *
1042AH
3000 U
Xba I
1093A
3000 U
EcoT22 I (Ava III) (NsiI)
1125A
2000 U
Xba I (HC)*
1093AH
3000 U
Fok I
1046A
1000 U
XspI (Bfa I, Mae I)
1095A
Hae III
1051A
4000 U
Hae III (HC) *
1051AH
Hap II (Hpa II, Msp I)
1053A
2000 U
Hap II (Hpa II, Msp I) (HC)*
1053AH
2000 U
4000 U
500 U
* HC = High Concentration
Notice to Purchaser. Your use of these products and technologies is subject to
compliance with any applicable licensing requirements described on the product’s
web page at http://www.clontech.com/takara. It is your responsibility to review,
understand and adhere to any restrictions imposed by such statements. Unless
otherwise specified, other trade names are also the trademarks or registered
trademarks of various companies.
TAKARA BIO INC.
www.clontech.com/takara