TM
1. Introduction
The Bay Laurel or Laurel (Laurus Nobilis) originally covered much of the
Mediterranean Basin and now widely spread in Europe and in Middle East
countries. Laurel is a genus of evergreen trees which belongs to the Laurel
family, Lauraceae. Dried leaves of Laurel also known as a Bay Leaf or Laurier.
It is also known that the leaf of Laurel has been widely used as a species in
cooking from the ancient times. In ancient Greece Laurel was sacred to Apollo
and was used to form a crown or wreath of honor for heroes, scholars and
poets. In USA Laurel generally recognized as safe (GRAS) by FDA. In Japan
it has been approved as a natural flavoring substance by the Ministry of Health
and Welfare.
Currently it has been reported that the periodontal diseases leads to a variety
diseases such as metabolic syndrome, cerebral vascular diseases, hearth
disease and pneumonia. It has been approved that the Laurel has a positive
effect in treatment of such a disease.
2. Active component of Laurel
Laurel contains 1-3% of essential oil such as cineole, eugenol, methyl
eugenol. As a active ingredient of periodontal disease, it contains Deacetyl
laurenobiolide.
O
O
OH
Deacetyl laurenobiolide
3.Antimicrobial activity of LAURESH
Compare with Porphyromonas gingivalis (P.g)、Actinomyces viscosus(A.v)、
Aggregatibacter actinomycetemcomitans (A.a) which is known as a typical
periodontal bacteria, It has been confirmed that the Laurel leaf extract also
has antimicrobial activity.
Table : Antimicrobial activity of LAURESH
Periodontal Pathogenic Bacteria
Minimal Inhibitory Concentration(MIC)
Porphyromonas gingivalis (P.g)
0.079mg/mL
Actinomyces viscosus (A.v)
0.318mg/mL
Aggregatibacter actinomycetemcomitans (A.a)
1.275mg/mL
Prevotella intermedia (P.i)
1.275mg/mL
Laurel leaf extract were diluted inside the test tube by adding various
periodontal bacteria. After culturing for certain period of time the minimum
inhibitory concentration (MIC) and minimum concentration showed no
growth of bacteria. Deacetyl laurenobiolide in compare with Porphyromonas
gingivalis (P.g) showed antibacterial activity of hinokitiol which is equivalent
to the fungicide periodontal disease.
4.Inhibitory effect of LAURESH on growth of
aerobic bacteria in the oral cavity.
Test Design
: Double – blind placebo control
Sample Intake : 1) Laurel gum (grain 18mg/ Laurel leaf extract)
2) Placebo gum
Trial Subject : 6 person (Laurel gum/3person, Placebo gum 3/person)
Intake Method : Laurel gum was chewed 2 days continuously 3 time in a day for
30min after the meal and brushing without the toothpaste.
Test Case
: Measurment of the number of anaerobic bacteria in the oral cavity
before and after chewing the gum.
40.0
The
numbe
rs of 30.0
anaero
bic
bacteri 20.0
a
（×100
10.0
0）
0.0
Placebo
gum
Gum containing
LAURESH
Before gum chewing
After gum chewing
Growth of oral bacteria suppressed after intake the laurel gum.
5. Safety
Acute toxicity：2000 mg/kg
6. Recomended dosage
100 mg/day (From monitor test results)
7. Specification
Deacetyl laurenobiolide 1.0 %
8. Contact Us
■Head Office/ Factory/ R&D
158 Kinoko, Sakura, Chiba 285-0801 Japan
Tel: 043-498-0007
Fax: 043-498-0561
■Tokyo Office
6F Nihonbashi-Uchiyama Bldg 4-4-16
Nihonbashi Honcho, Chuo-Ku, Tokyo 103-0023 Japan
Tel: 03-5200-1251
Fax: 03-5200-1256
■http://www.tokiwaph.com/
■ E-mail [email protected]