Chromosome specimen making
Metaphase Harvester
Chromosome specimen maker Metaphase Harvester HANABI-PII does the
processing of cell harvesting from the cultured cell automatically, simplifying a
complicated process and producing uniform cells. The main operation of
Metaphase Harvester is the constant temperature centrifugal separator structure, a
hypotonic treatment using pre-heated hypotonic solution that enable fixation
processing with cooled fixative solution. The centrifugal separator section is
located at the center with 6 sets of centrifugal
sections, 4 tubes per set, capable of producing
24 specimens at one time by injecting
solutions and exhaust solutions.
By using the HANABI-PII, a large amount
of stable chromosome specimens can be
produced enabling cell harvesting.
Centrifuge operation section
Manufacturer and Distributor
HANABI-PII
Sales Agency
110524
HANABI-PII Specifications
The main specifications are as follows.
Items
Specifications
Remarks
1
Centrifugal tube
15 ml
FALCON
2
Culture cell suspensions
Max. 10 ml
Typical 5ml
3
Number of samples
Max. 24 samples
8, 12, 16, 24 samples
4
Total processing time
Approx. 2.0hrs
Hypotonic: 30min.
Samples: 24
5
Centrifuge
Approx. 1000 rpm
Approx. 230G
6
Acetic acid : Methanol
1:3
7
Hypotonic treatment temperature
37 degrees
8
Centrifuge section temperature
37 degrees
Select ON/OFF
9
Operating environment
15-30 degrees
Humidity（20-60%RH）
10
Power consumption
AC120V – 800W
50/60Hz (factory select)
11
Externals size (WxHxD)
990Wx1560Hx710D
12
Weight
Approx. 370 Kg
13
Safety Standards
CE-marking
EN61010-1, EN61000-4
2005/12/10
HANABI-PII FLOW
Process
A
B
Centrifugation
1000 rpm
5 min
Range (1-10 min)
C
Supernatant
Injection
Aspiration and (Hypotonic Solution)
Tube Washing Range 1-6 ml (0.25step)
Injection Volume (C)
Example
5.0ml
E
Stirring
5 sec
H
G Process Notes
Injection Volume (G)
0.25-5 ml ( 0.25 ml step )
G process can be
skipped by the setting.
First fixative
Stir
Stir
Hypotonic Treatment
Repeat 0-1 time
J Process Setting
G
Aspiration Level
Setting Value (B)
Example: 1.0 ml
Stir
Injection Volume (Fixative)
(Range: 1-5 ml 0.25step)
Setting examples
(1) (2)
(J1) 5.0 3.0
(J2) 5.0 5.0
(J3) 5.0 5.0
(J4) 1.5 1.5
(J5) 1.5 1.5
F
Hypotonic
Injection
Centrifugation
Treatment
(Fixative Solution)
1000 rpm
37℃ Range 0.25-5 ml (0.25step)
5 min
Injection Volume (G) Range(1-10 min)
Total Hypotonic Treatment Time
(1)Total = Process (C,D,E,F,G)
(2)Total = Process (C,D,E,F,H,I,J) @G-Skip
Range: 10-50 min
Stirring
After
Injection
Cultured Cell
Cell
Suspension
10 ml (max)
D
Hypotonic
Treatment
37℃
I
Supernatant Aspiration
and Tube Washing
(Stirring After or Before Injection)
J
K
L
Injection
(Fixative Solution)
Range1-5 ml (0.25step)
Injection Volume (J1-J5)
Centrifugation
1000 rpm
2.5 min
Range (1-10 min)
End of harvesting
Metaphase
Spreader
I Process Setting
Aspiration Level
(Range: 0.2-5 ml)
Setting examples
(1) (2)
(I1) 1.0 4.0
(I2) 0.5 1.0
(I3) 0.2 0.5
(I4) 0.2 0.2
(I5) 0.2 0.2
Aspiration Level
1: Set value (I1)
2: Set value (I2)
3: Set value (I3)
4: Set value (I4)
5: Set value (I5)
Repeats 0-4 times
HANABI
J Process Operation
Stir
J1: (G-Skip) Stirring Before Injection
J1: (G-Exe.) Stirring After Injection
J2-J5: Stirring After Injection
Range（1-20 sec)
ADSTEC Corporation
Please use HANABI for the spreading of the chromosomes.
110524