page 52
Lab Times
Products
4-2016
Western Blotting Transfer Systems
Company/Distributor Name of
Transfer
Technology
Capacity
Format
Transfer Time
Miscellaneous, Specialities, Generally
Price [EUR]
Bio-Budget
Technologies
Semi-dry blotting
11 x 11 cm
10 min up to 2 hours
High quality plate electrodes for homogeneous transfer |
Robust construction made of 9 mm acryl glass | Safety
lid with three easy-to-close and stable screw buttons |
Only 50 ml of transfer buffer required | 3 years warranty
795.–
Semi-dry blotting
21 x 21 cm
10 min up to 2 hours
High quality plate electrodes for homogeneous transfer | 1,150.–
Robust construction made of 9 mm acryl glass | Safety
lid with three easy-to-close and stable screw buttons |
Only 200 ml of transfer buffer required | 3 years warranty
Tank Blotter Mini
Tank blotting
9 x 9 cm
1–4 gels
20 min to several hours
High quality platin electrodes for homogeneous transfer | 1,050.–
Robust construction made of 9 mm acryl glass | Prepared
for water cooling | Colour-coded compression cassettes
with fiber pads | 3 years warranty
Tank Blotter
Tank blotting
18 x 20 cm
2 gels
20 min to several hours
High quality platinum plate electrodes for homogeneous
1,375.–
transfer | Robust construction made of 9 mm acryl glass
| Prepared for water cooling | Colour-coded compression
cassettes with fiber pads | 3 years warranty
Trans-Blot Turbo
Rapid blotting
system
2 independent cassettes to
transfer 4 x mini gels
(10 x 7.5 cm) or
2 x midi gels (15 x 9.4 cm)
3–30 min
Rapid, high through-put transfers | Ready-to-use transfer
packs available with nitrocellulose and PVDF membranes
| Ready-to-assemble kits with pre-cut membranes,
filter pads with nitrocellulose, PVDF or low fluorescence
PVDF membranes | Flexible design also accommodates
traditional semi-dry consumables | Environmentally safe
single use consumables to reduce waste and waste costs
1,990.–
Mini-Trans Blot Cell
Tank transfer
system
Up to 2 mini gels
(10 x 7.5 cm)
1 hour to overnight
Use with pre-cast or hand-cast gels | Colour-coded cassettes and electrodes ensure proper orientation of the gel
during transfer | Blue cooling unit absorbs heat generated
during rapid transfers | Available as a stand-alone apparatus or as a module to use with the Mini-Protean Tetra
cell system | PowerPac HC recommended (at extra cost)
1,671.–
(stand alone
apparatus)
861.– (blotting module)
Criterion Blotter
Tank transfer
system
Up to 4 mini gels
(10 x 7.5 cm) or
2 midi gels (15 x 9.4 cm)
30 min to 1 h
Use with pre-cast or hand-cast gels | Plate electrodes
available for faster, more efficient transfers (wire electrodes are also available) | Sealed ice block included but
optional cooling coil can be purchased | PowerPac HC
recommended (at extra cost)
1,115.– (incl.
tank & plate
electrodes
873.– (system with wire
electrodes)
Trans-Blot SD
Semi-dry transfer
system
4 x mini gels (10 x 7.5 cm) or
3 x (15 x 9.4 cm)
Maximum gel size of 24 x
16 cm
15–60 min
Fast and efficient blotting without buffer tank or gel
cassettes | Minimal buffer requirement | Can transfer
multiple gel sizes | Robust , simple design for simple use
and durability | PowerPac HC or similar microprocessorcontrolled power supply is required ( at extra cost)
1,354.– (Trans-Blot SD only) 2,369.– (with PowerPac HC)
Trans-Blot Cell
Tank transfer
system
Up to 12 mini gels or 6 midi
gels at the same time
For higher capacity blotting
Please
enquire
Trans-Blot Plus Cell
Tank transfer
system
Up to 27 mini gels/12 midi gels For higher through-put blotting
at the same time
15–30 min
Fastblot B33/B34
16 x 20 cm
Fastblot B43/B44
Semi-dry blotting
of proteins up to
150 KDa
Cooling option (Fastblot B33) | Carbon-based electrodes
| Blotting times are reduced to 10 to 30 min
Cooling option (Fastblot B43) | Maintenance-free metal
electrodes
Please
enquire
Tankblot Eco-Mini
Tank blotting
4 gels
9.2 cm x 9.5 cm (max.)
4 separate Blotting Cassettes for single gels |
Buffer tank with integrated cooling base
Please
enquire
Tankblot Eco-Maxi
Tank blotting
2 gels
22 cm x 19 cm (max.)
Buffer tank with integrated cooling base | A stir bar may
be added to the buffer tank to further improve buffer
circulation and heat exchange
Please
enquire
Dot Blot 96
Vacuum blotting
96-well format
Innovative sealing system | No cross-contamination |
Microtiter plate / multichannel pipette compatible
Please
enquire
Gelco Electroblot-
Wet blotting
system
Mini: Up to 5 blots 10 x 10 cm Cost-effective, safe and easy-to-use system |
Maxi: Up to 5 blots 20 x 20 cm Plate electrodes for a higher strength electric field |
Rapid gel transfers | Flat bottom allows to be placed
or 20 blots 10 x 10 cm
directly onto a magnetic stirrer
Product
Semi-Dry Electroblotter 10 x 10
Krefeld, Germany
www.biobudget-shop.de
Semi-Dry ElectroContact:
Phone +49 2151 6520830 blotter 20 x 20
[email protected]
Bio-Rad Laboratories
Transfer System
Muenchen
www.bio-rad.com
Contact:
Phone + 49 89 31884 177
[email protected]
Biometra
Goettingen, Germany
www.biometra.com
Contact:
Phone +49 551 50686 0
[email protected]
Biostep
ting Systems
Burkhardtsdorf, Germany
www.biostep.de
Contact: Silvana Böhme
Phone +49 3721 3905 24 Gelco Semi Dry
Systems
[email protected]
Biozym Scientific
Hessisch Oldendorf,
Germany
www.biozym.com
Contact: Anja Röben
Phone: +49 5152 9020
[email protected]
LT_416_52_54.indd 52
Please
enquire
From 526.–
Semi dry systems Mini: 10 x 10 cm: 1 gel
Maxi: 20 x 20 cm: 4 gels à 10
x 10 cm or 1 gel à 20 x 20 cm
Gels are stackable for higher throughput blotting |
Colour-coded, corrosion-free plate electrode |
Large active transfer area | Fast transfer time
From 595.–
EasyPhor PAGE
WetBlotter Mini
System
Wetblotting (tank) 10 x 10 cm
4 cassettes, 16 fibre pads
1–2 h
Uniform electric field | Rigid cassettes maximise current
transfer | Up to 4 blots parallel | Universal tank and lid –
can also be used with EasyPhor electrophoresis module
509.–
EasyPhor PAGE
WetBlotter Mini
Wide System
Wetblotting (tank) 20 x 10 cm
3 cassettes, 12 fibre pads
5–20 h
Uniform electric field | Rigid cassettes maximise current
transfer | For 3 Blots with 20 x 10 cm or 6 blots with
10 x 10 cm | Universal tank and lid – can also be used
with EasyPhor electrophoresis module
729.–
05.08.16 11:41
Products
4-2016
Lab Times
page 53
Western Blotting Transfer Systems
Company/Distributor Name of
Product
Transfer
Technology
Biozym Scientific
EasyPhor PAGE
Wetblotter Maxi
WAVE System
(continued)
Contact: see page 52
Carl Roth
Karlsruhe, Germany
www.carlroth.de
Contact: Stefanie Seipp
Phone:
+49 721 5606 1038
[email protected]
C.B.S. Scientific
Company
Del Mar (CA), USA
www.cbsscientific.com
Contact:
Phone +1 858 755 4959
[email protected]
Dunn Labortechnik
Asbach, Germany
www.dunnlab.de
Contact: Andreas Bichet
Phone +49 26 83 430 94
[email protected]
LT_416_52_54.indd 53
Capacity
Format
Transfer Time
Miscellaneous, Specialities, Generally
Price [EUR]
Wetblotting (tank) 4 cassettes, 16 fibre pads
5–20 h
20 x 20 cm
Uniform electric field | Rigid cassettes maximise current
transfer | Up to four 20 x 20 cm blots parallel |
Universal tank and lid – can also be used with EasyPhor
electrophoresis module
734.–
EasyPhor PAGE
WetBlotter Mini HI
System
High intensity
wetblotting
(tank with plate
electrodes)
2 cassettes, 8 fibre pads
in general < 1 h
10 x 10 cm
Rigid cassettes maximise current transfer | Platinum
coated plate electrodes generate a powerful electric field
for fast and efficient transfer | For two 10 x 10 cm blots
parallel | Universal tank and lid – can also be used with
EasyPhor electrophoresis module
579.–
EasyPhor PAGE
WetBlotter Mini HI
System
High intensity
wetblotting
(tank with plate
electrodes)
1 cassette, 6 fibre pads
in general < 1 h
20 x 20 cm
Rigid cassettes maxi-mise current transfer | Platinum
853.–
coated plate electrodes generate a powerful electric field
for fast and efficient trans-fer | For one 20 x 20 cm blot |
Universal tank and lid – can also be used with EasyPhor
electrophoresis module
Rotiphorese
PROclamp Tank
Blotting System
Tank blotting
4 gels (Mini: max. 10 x 10 cm,
Maxi: max. 20 x 20 cm)
60 min (RT) to 12 h
(cooled, big proteins)
Very even and gentle transfer, even for big-sized
proteins | For denaturing and native blots | Coolable
buffer tank | Sophisticated design, for very easy handling
| Compatible with Rotiphorese PROclamp Vertical
Electrophoresis System
785.–/Mini
1,210.–/Maxi
Special prices
on request
Semi Dry Blotter
Semi dry blotting
Mini: 3 gels of max. 10 x 10
cm (piled)
30–60 min
Maxi: 3 gels of max. 20 x 20
cm (piled) or 3 x 4 gels of max.
10 x 10 cm (4 piles of 3 gels
each)
60-120 min
Fast, steady and efficient transfer | Very little heat
development | Low buffer use, may also be used with
discontinuous buffer systems | Durable, corrosion-free
electrodes (stainless steel and platinised titanium) | Very
elegant design and most simple handling w/o the necessity of lid fixation
759.– (Mini)
1,499.–
(Maxi)
Special prices
on request
Semi Dry Blotter
PROfessional
Semi dry blotting
Mini: 3 gels of max. 10 x 10
cm (piled)
30-60 min
Maxi: 3 gels of max. 20 x 20
cm (piled) or 3 x 4 gels of max.
10 x 10 cm (4 piles of 3 gels
each)
60–120 min
Fast, steady and efficient transfer | Very little heat
development | Low buffer use, may also be used with
discontinuous buffer systems | Durable, corrosion-free
electrodes (stainless steel and platinised titanium) | Very
simple handling including lid fixation by screws (optional)
689.– (Mini)
1,625.–
(Maxi)
Special prices
on request
Dot Blotter or Slot
Blotter
Vacuum transfer
Dot blotter: 96 samples (8 x
12, 96 well format), 180 µl
each
Approx. 30 s
Slot blotter: 48 samples
(3x16), 360 µl each
Approx. 1 min
Regular transfer without leaking | Very easy assembling
| Alpha numerical labelling of sample wells | Made of
robust acrylic glass | Use with water- or membrane pump
(200 mbar vacuum)
665.– (Dot
blotter)
709.– (Slot
blotter)
Special prices
on request
EBU-204P
Tank blotting
10 x 10 cm (max.)
With plate electrode panels, for Western, Southern or North- Please conern Blots | Tank blotter includes internal cooling base, tact your local
1 gel cassette and safety cover with attached power leads distributor
EBU-102
Tank blotting
15 x 21.5 cm
3 Gel cassettes
Tank blotter includes platinum wire electrode panels,
Please coninternal cooling base, 1 gel cassette and safety cover with tact your local
attached power leads
distributor
EBU-102P
Tank blotting
15 x 21.5 cm
3 Gel cassettes
With planar electrode panels | Tank blotter includes internal Please concooling base, 1 gel cassette and safety cover with attached tact your local
power leads
distributor
EBU-222
--
20 x 20 cm
2 Gel cassettes
Tank blotter includes platinum wire electrode panels, in- Please conternal cooling base, 1 gel cassette and safety cover with tact your local
attached power leads
distributor
Various Mini blotting systems and
Western blotting
bundles
Tank blotting
--
--
Please contact your local
distributor
EBU-4000
Semi-dry blotting
20 x 20 cm (max.)
Includes stainless steel cathode and platinum-coated
titanium anode, interlocking power leads with safety-tips
and Mylar sheets
Please contact your local
distributor
EBU-6000
Semi-dry blotting
35 x 45 cm (max.)
Includes stainless steel cathode and platinum-coated
titanium anode, interlocking power leads with safety-tips
and Mylar sheets
Please contact your local
distributor
EBU-3000
Semi-dry blotting
12 x 34 cm (max.)
Includes stainless steel cathode and platinum-coated
titanium anode, interlocking power leads with safety-tips
and Mylar sheets
Please contact your local
distributor
MaGELin Western
Transfer System
Wet transfer
2 gels (8 x 10 cm,
or 10 x 10 cm)
60–90 min
Complete system with cassette module, vented lid, buffer
tank, and power cords
Approx. 900.–
Semi-Dry Blotter
Semi dry transfer
24 x 30 cm
Up to 6 transfer units
Graphite-coated plates to reduce heat and minimize
buffer usage
Approx.
1,500.–
05.08.16 11:41
page 54
Lab Times
Products
4-2016
Western Blotting Transfer Systems
Company/Distributor Name of
Transfer
Technology
Capacity
Format
Transfer Time
Miscellaneous, Specialities, Generally
Price [EUR]
GE Healthcare
TE 70 PWR SemiDry Transfer Unit
Semi-dry blotting
14 x 16 cm (max.) or
two mini gels side-by-side
Electrotransfers proteins from polyacrylamide gels in less
than 1 h | Tested for use with Amersham ECL Western
blotting reagents | Units are available with or without
power supply | Minimal buffer needed, only enough to
saturate blotting papers and membranes
Please
enquire
TE 77 Semi-Dry
Transfer Unit
Semi-dry blotting
21 x 26 cm (max.) or
four side-by-side mini-gels
See above
Please
enquire
Amersham WB
System
--
--
Fully integrated system for SDS-PAGE and Western blotting of proteins based on fluorescence detection | The system provides standardised, quantitative, and
reproducible analysis of proteins | Reliable normalization
by multiplexing target and control fluorescent signals on
the same blot | Western blot workflow achieved in less
than 4 h ensures fast, conclusive data
Please
enquire
Product
Freiburg, Germany
www3.gehealthcare.de
Contact:
[email protected]
TE70XP
Semi dry
Holliston (MA), USA
www.hoeferinc.com
Contact:
Phone: +1 508 893 8999
[email protected]
14 x 16 cm
Up to four mini gels
Intelligent built-in power supply, which automatically
monitors the transfer status, stopping the transfer before
the stack overheats
Please contact your local
distributor
TE77XP
Semi dry
Up to 12 mini
21 x 26 cm
gels at one time
See above
See above
TE42 Standard
Transfer Tank
Wet blotting
Up to four gels
Quickly and evenly transfers proteins and nucleic acids
from polyacrylamide or agarose gels onto nylon, nitrocellulose or PVDF membranes
Please contact your local
distributor
ProteinSimple
Wes
Separation and
immunoprobing
in capillary
Up to 25 samples per run
Separate and detect proteins as large as 440 kDa in as
little as 3 hours
Please
enquire
Sally Sue
See above
Up to 96 samples per run
Up to 96 samples in one experiment with as little as
0.2 µg/µl protein in the sample
Please
enquire
Peggy Sue
See above
Up to 96 samples per run
Separate and analyze proteins by size or charge from
12 to 440 kDa either by immunoassay or total protein
Please
enquire
Milo
On-chip SDSPAGE, immobilisation and
immunoprobing
scWest chip partitions the
cells and captures ~1,000
individual cells
Single cell Western | Resolves protein isoforms that can’t
be distinguished with flow cytometry
Please
enquire
BlueBlot Semi-Dry
Semi-dry blotting
11 x 11 cm
Platinum-covered steel net as anode | Spring-mounted
anode for blotting stacks | Stainless steel plate as
cathode | Deployable for thicker gels and blotting stacks
Please
enquire
Semi-dry blotting
17 x 17 cm
Platinum-covered steel net as anode | Spring-mounted
anode for blotting stacks | Stainless steel plate as
cathode
Please
enquire
Semi-dry blotting
24 x 26 cm
Platinum-covered steel net as anode | Spring-mounted
anode for blotting stacks | Stainless steel plate as
cathode
Please
enquire
Up to 4 mini gels or 2 midi
gels
5–10 min
High transfer efficiency with a broad range of protein sizes 2,050.–
(10–300 kDa) compared to conventional semi-dry or wet (tank) transfer methods | Transfer of proteins in 5 to 10
min when used with 1-Step Transfer Buffer | Integrated
power supply | Easy-touch programming
Hoefer
San Jose (CA), USA
www.proteinsimple.com
Contact:
Phone +1 408 510 5500
[email protected]
Serva Electrophoresis
Blotter SD 11
Heidelberg, Germany
www.serva.de
Contact:
BlueBlot Semi-Dry
Phone +49 6221 13840 0 Blotter SD17
[email protected]
BlueBlot Semi-Dry
Blotter SD26
Thermo Fisher
Scientific
Paisley, United Kingdom
www.thermofisher.com
Contact: Olivier Eder
Phone +33 660 216 552
VWR International
Darmstadt, Germany
https://de.vwr.com
Contact:
Phone +49 6151 39 72 0
[email protected]
LT_416_52_54.indd 54
Semi dry
Thermo Scientific
Pierce Power Blotter
Thermo Scientific
Invitrogen iBlot2
Dry
Up to 2 mini gels or 1 midi gel
7 min or less
High-quality and more compact transfer stacks | In1,534.–
creased blotting reliability and reproducibility | Flexible
gel-size formats and membrane types | Simple, userfriendly system | Ability to create new custom programs |
Built-in tutorial and application notes
Thermo Scientific
Invitrogen Bolt Mini
Blot Module
Wet
1 mini gel per module
30–60 min
Universal module design — allows modules to fit in either 355.–
chamber of the tank, simplifying the transfer setup |
Unique gasket seal to prevent buffer leakage so there is
no mess during setup of your Western transfer | 1/2 inch
buffer chamber requires only half the volume of methanolbased transfer buffer | Standard 60 min transfer protocol
accelerates western workflow | Robust electrodes, sturdy
steel plates
Perfect Blue Sedec
Semi-dry blotting
1h
Stainless steel cathode, platinum-covered titanium anode Please
enquire
Perfect Blue Web S
and M
Tank blotting
Up to 4 blotting cassettes
Gel sizes: 18 x 20 cm (M),
8.5 x 9.5 cm (S)
Colour-coded blotting cassettes | Integrated cooling
system
Diverse Western
blotting apparatuses from different
manufacturers
Please
enquire
Please
enquire
05.08.16 11:41