GENERATION OF FUNCTIONAL HUMAN ORGAN FROM
PLURIPOTENT STEM CELL
Hideki Taniguchi, M.D., Ph.D. and Takanori Takebe, M.D.
Department of Regenerative Medicine, Yokohama City University
Graduate School of Medicine, JAPAN
ABSTRACT
Organ transplantation is the only curative method for treating end-stage organ
failure. Over the past decade, there has been increased demand for organ
transplantation throughout the world owing to the increased incidence of organ
failure. To address such important clinical issue, a critical shortage of donor
organs highlights the urgent need for generating organs from human pluripotent
stem cells. Here we show the generation of functional human organ from human
induced pluripotent stem cells (iPSCs) by transplantation of organ buds created in
vitro. Human vasculatures in iPSC-organ bud transplants became functional by
connecting to the recipient’s vessels. The formation of functional vasculatures
stimulated the maturation of iPSC-organ bud into tissue resembling the adult organ.
This method is the alternative way to the generation of functional human organ from
pluripotent stem cells. Although efforts must ensue to translate these techniques
to treatments for patients, this proof-of- concept demonstration of organ-bud
transplantation provides a promising new approach to a critical shortage of donor
organs for treating end-stage organ failure.
KEYWORDS:
Organ transplantation
INTRODUCTION
Please refer to the abstract.
THEORY
In the organogenesis process, co-operative interaction is caused by multi-cellular
system taking organized tissue structure, and the true functional cells, which finally
accomplished terminal differentiation while being accompanied by the change of the
interval in dynamic space and time, are brought about. The conventional way of
thinking to which a cell of the purpose is led is reconsidered radically to achieve
creation of a function cell, and it is necessary to establish a terminal differentiation
derivative method with the reconstitution of the three-dimensional high-level
structure that imitated in vivo organogenesis.
EXPERIMENTAL
Please refer to the references（1-3）.
978-0-9798064-8-3/µTAS 2015/$20©15CBMS-0001
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19th International Conference on Miniaturized
Systems for Chemistry and Life Sciences
October 25-29, 2015, Gyeongju, KOREA
RESULT AND DISCUSSION
Please refer to the references（1-3）.
CONCLUSION
Proof-of-concept organ bud transplantation might be a rational approach toward
the generation of vascularized and functional complex organs. This culture principle
will not only be a powerful tool for the study of human developmental biology and
pathol- ogy using pluripotent stem cells, but it will also facilitate the realization of
next-generation generative medicine using in-vitro-grown complex tissue
architectures for currently un- treatable patients.
ACKNOWLEDGEMENTS
This work was supported by grants to H. Taniguchi from the Strategic Promotion
of Innovative Research and Development (S-innovation, 62890004) of the Japan
Science and Technology Agency (JST). This work was also supported by the
Grants-in-Aid of the Ministry of Education, Culture, Sports, Science, and
Technology of Japan. This work was also partly supported by the JST through its
research grant Research Center Network for Realization of Regenerative Medicine.
T. Takebe and H.T. have served on scientific advisory boards for Healios Inc. T.
Takebe and H.T. have also granted a license to Healios through Yokohama City
University over their inventions that relate to the subject of this manuscript. T.Takebe
and H.T. are conducting research under the collaborative research agreement with
Healios and the subject of the research also relates to the subject of this manuscript.
REFERENCES
1. Takebe T, Enomura M, Yoshizawa E, Kimura M, Koike H, Ueno Y, Matsuzaki T,
Yamazaki T, Toyohara T, Osafune K, Nakauchi H, Yoshikawa HY, Taniguchi H :
Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal
Cell-Driven Condensation. Cell Stem Cell 16: 556-565, 2015
2. Takebe T, Zhang RR, Koike H, Kimura M, Yoshizawa E, Enomura M, Koike N, Sekine K,
Taniguchi H :Generation of a vascularized and functional human liver from an
iPSC-derived organ bud transplant. Nature Protocols 9:396-409, 2014
3. Takebe T, Sekine K, Enomura M, Koike H, Kimura M, Ogaeri T, Zhang RR, Ueno Y,
Zheng YW, Koike N, Aoyama S, Adachi Y, Taniguch H :Vascularized and functional
human liver tissue from an induced pluripotent stem cell-derived organ bud transplant.
Nature 499:481-484,2013
CONTACT
Hideki Taniguchi; phone:+81-45-787-2621; [email protected]
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